CN106234220A - A kind of method of Beijing 553 Sweetpotato Viruses Elimination nursery - Google Patents
A kind of method of Beijing 553 Sweetpotato Viruses Elimination nursery Download PDFInfo
- Publication number
- CN106234220A CN106234220A CN201610632538.3A CN201610632538A CN106234220A CN 106234220 A CN106234220 A CN 106234220A CN 201610632538 A CN201610632538 A CN 201610632538A CN 106234220 A CN106234220 A CN 106234220A
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- CN
- China
- Prior art keywords
- rhizoma dioscoreae
- dioscoreae esculentae
- beijing
- nursery
- sterilization
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Hydroponics (AREA)
Abstract
nullThe present invention is a kind of method of Beijing 553 Sweetpotato Viruses Elimination nursery，Its incubation step is: first choose Rhizoma Dioscoreae esculentae block，It is passivated virus treated and disinfects，Put into and fine sand carries out accelerating germination，Then，The stem apex of the eugonic 3cm of clip when the seedling length of Rhizoma Dioscoreae esculentae block is to 30cm，Cut blade，Stem apex is carried out disinfection，Strip under anatomical lens afterwards with a piece of phyllopodium 0.2～0.3mm growing point，It is inoculated in MS culture medium，Then culture dish is put in group training room and cultivates，Viral diagnosis is carried out when stem apex develops into the plantlet with 4 blades，Leave healthy virus-free plant，Finally intercept the stem section of 4cm，Insert in the hole tray filling substrate，Put into booth and carry out nursery，Present invention achieves the detoxification nursery of Beijing 553 Rhizoma Dioscoreae esculentae，The cultivation of nontoxic Rhizoma Dioscoreae esculentae Seedling can be effectively realized，Prevent variety deterioration，Improve yield and the quality of Rhizoma Dioscoreae esculentae.
Description
Technical field
The present invention relates to that plant tissue culture is the most numerous and detoxification field, particularly relate to a kind of Beijing 553 Sweetpotato Viruses Elimination nursery
Method.
Background technology
Rhizoma Dioscoreae esculentae is one of main cereal crops, is also important forage crop simultaneously, has the highest economic worth, because of
This cultivated area is more and more extensive, and wherein, Beijing 553 Rhizoma Dioscoreae esculentae is one of sweet potato variety of edible-type, potato block spindle, color of the leather Fructus Citri tangerinae
Yellow.But Beijing 553 Rhizoma Dioscoreae esculentae is easily infected by multiple virus and pathogen, thus cause the reduction of kind of a sexual involution, yield, quality
Degenerate, especially when establishing in large scale, be susceptible to large area Rhizoma Dioscoreae esculentae and caused huge economic loss by the harm of virus.Plant
Thing Shoot-tip Grafting In Vitro is the characteristic utilizing growing tips of the plant not contain or containing very small amount virus, takes shoot apical meristem technology,
Breeding of virus-free or virus removal plant can be realized, therefore, utilize this technology also can realize colory nontoxic Beijing 553
The cultivation of Rhizoma Dioscoreae esculentae Seedling.
Summary of the invention
Present invention seek to address that the deficiencies in the prior art, and a kind of method that Beijing 553 Sweetpotato Viruses Elimination nursery is provided.
The present invention for achieving the above object, by the following technical solutions: a kind of method of Beijing 553 Sweetpotato Viruses Elimination nursery,
It is characterized in that, its step is as follows:
(1) choose Beijing 553 Rhizoma Dioscoreae esculentae block of the same size, be placed in the water-bath of alternating temperature carrying out warm water passivation virus
Process；
(2) carry out the Rhizoma Dioscoreae esculentae block after warm water passivation virus treated and put into soaking disinfection in sterilization medicinal liquid, sweet by handle well
Potato block is put in container, covers the fine sand disinfected, and carries out accelerating germination in being placed in hot house；
(3) when the seedling length of Rhizoma Dioscoreae esculentae block is to 30～40cm, clip eugonic 3～the stem apex of 5cm, cut blade；
(4) stem apex cutting blade is carried out disinfection, during sterilization first with 70% soak with ethanol 30s, then use sterilized water
Rinse one time, then soak 8min with the mercuric chloride solution of 0.1%, re-use aseptic water washing four times；
(5) under anatomical lens, the stem apex after sterilization is stripped the growth of the 0.2～0.3mm size with a piece of phyllopodium
Point, is seeded in MS culture medium；
(6) postvaccinal culture dish be put into group training room in cultivate, cultivation temperature is 25 DEG C, intensity of illumination 2000～
3000LX, light application time 16h/d, when stem apex develops into the plantlet with 4～5 blades, by serum method, plantlet is entered
Row Viral diagnosis, removes and still carries virulent plant；
(7) in superclean bench, intercept the stem section of 4cm with the dissecting knife after sterilization, retain a piece of of each stem section top
Launch leaf, insert after removing the blade of bottom in the hole tray filling sterilization substrate；
(8) hole tray being plugged Seedling is put in booth, enclosed fly net, regular watering, nontoxic Beijing 553 can have been realized sweet
The cultivation of potato Seedling.
Particularly, the passivation treatment method of virus in described step (1) is: first water-bath is warming up to 57 DEG C, Rhizoma Dioscoreae esculentae
Block soaks 3min, then water temperature is down to 51 DEG C, and Rhizoma Dioscoreae esculentae block is soaked 30min.
Particularly, 50% carbendazim that sterilization medicinal liquid is dilution 800 times in described step (2).
The invention has the beneficial effects as follows: the present invention, in incubation step, has carried out warm water virus to Beijing 553 Rhizoma Dioscoreae esculentae block blunt
Change processes, and fractionated viral can be made to lose infection ability, present invention achieves Beijing 553 Sweetpotato Viruses Elimination nursery, can be effectively
Realize the cultivation of nontoxic Beijing 553 Rhizoma Dioscoreae esculentae Seedling, prevent variety deterioration, improve yield and the quality of Rhizoma Dioscoreae esculentae.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
A kind of method of Beijing 553 Sweetpotato Viruses Elimination nursery, it is characterised in that its step is as follows:
(1) choose Beijing 553 Rhizoma Dioscoreae esculentae block of the same size, be placed in the water-bath of alternating temperature being passivated at virus
Reason, method is: first water-bath is warming up to 57 DEG C, Rhizoma Dioscoreae esculentae block is soaked 3min, then water temperature is down to 51 DEG C, and Rhizoma Dioscoreae esculentae block is soaked
Bubble 30min；
(2) carry out in the 50% carbendazim sterilization medicinal liquid that the Rhizoma Dioscoreae esculentae block after warm water passivation virus treated puts into dilution 800 times
Soaking disinfection 15min, puts in container by the Rhizoma Dioscoreae esculentae block handled well, covers the thick fine sand disinfected of 10cm, and being placed in temperature is
Accelerating germination is carried out in the hot house of 30 DEG C；
(3) when the seedling length of Rhizoma Dioscoreae esculentae block is to 30cm, the stem apex of the eugonic 3cm of clip, cut blade；
(4) stem apex cutting blade is carried out disinfection, during sterilization first with 70% soak with ethanol 30s, then use sterilized water
Rinse one time, then soak 8min with the mercuric chloride solution of 0.1%, re-use aseptic water washing four times；
(5) under anatomical lens, the stem apex after sterilization is stripped the growing point of the 0.2mm size with a piece of phyllopodium, inoculation
In MS culture medium；
(6) postvaccinal culture dish be put into group training room in cultivate, cultivation temperature is 25 DEG C, intensity of illumination
2000LX, light application time 16h/d, when stem apex develops into the plantlet with 4 blades, by serum method, plantlet is carried out disease
Poison detection, removes and still carries virulent plant；
(7) in superclean bench, intercept the stem section of 4cm with the dissecting knife after sterilization, retain a piece of of each stem section top
Launch leaf, insert in the hole tray filling sterilization substrate after removing the blade of bottom, insertion depth 2cm；
(8) hole tray being plugged Seedling is put in booth, enclosed fly net, regular watering, nontoxic Beijing 553 can have been realized sweet
The cultivation of potato Seedling.
Embodiment 2
A kind of method of Beijing 553 Sweetpotato Viruses Elimination nursery, it is characterised in that its step is as follows:
(1) choose Beijing 553 Rhizoma Dioscoreae esculentae block of the same size, be placed in the water-bath of alternating temperature being passivated at virus
Reason, method is: first water-bath is warming up to 57 DEG C, Rhizoma Dioscoreae esculentae block is soaked 3min, then water temperature is down to 51 DEG C, and Rhizoma Dioscoreae esculentae block is soaked
Bubble 30min；
(2) carry out in the 50% carbendazim sterilization medicinal liquid that the Rhizoma Dioscoreae esculentae block after warm water passivation virus treated puts into dilution 800 times
Soaking disinfection 15min, puts in container by the Rhizoma Dioscoreae esculentae block handled well, covers the thick fine sand disinfected of 10cm, and being placed in temperature is
Accelerating germination is carried out in the hot house of 30 DEG C；
(3) when the seedling length of Rhizoma Dioscoreae esculentae block is to 40cm, the stem apex of the eugonic 5cm of clip, cut blade；
(4) stem apex cutting blade is carried out disinfection, during sterilization first with 70% soak with ethanol 30s, then use sterilized water
Rinse one time, then soak 8min with the mercuric chloride solution of 0.1%, re-use aseptic water washing four times；
(5) under anatomical lens, the stem apex after sterilization is stripped the growing point of the 0.3mm size with a piece of phyllopodium, inoculation
In MS culture medium；
(6) postvaccinal culture dish be put into group training room in cultivate, cultivation temperature is 25 DEG C, intensity of illumination
3000LX, light application time 16h/d, when stem apex develops into the plantlet with 5 blades, by serum method, plantlet is carried out disease
Poison detection, removes and still carries virulent plant；
(7) in superclean bench, intercept the stem section of 4cm with the dissecting knife after sterilization, retain a piece of of each stem section top
Launch leaf, insert in the hole tray filling sterilization substrate after removing the blade of bottom, insertion depth 2cm；
(8) hole tray being plugged Seedling is put in booth, enclosed fly net, regular watering, nontoxic Beijing 553 can have been realized sweet
The cultivation of potato Seedling.
Above the present invention is exemplarily described, it is clear that the present invention implements and is not subject to the restrictions described above,
As long as have employed method design and the various improvement that carry out of technical scheme of the present invention, or the most improved directly apply to other
Close, all within protection scope of the present invention.
Claims (3)
1. the method for a Beijing 553 Sweetpotato Viruses Elimination nursery, it is characterised in that its step is as follows:
(1) choose Beijing 553 Rhizoma Dioscoreae esculentae block of the same size, be placed in the water-bath of alternating temperature carrying out at warm water passivation virus
Reason；
(2) carry out the Rhizoma Dioscoreae esculentae block after warm water passivation virus treated and put into soaking disinfection in sterilization medicinal liquid, the Rhizoma Dioscoreae esculentae block that will handle well
Put in container, cover the fine sand disinfected, in being placed in hot house, carry out accelerating germination；
(3) when the seedling length of Rhizoma Dioscoreae esculentae block is to 30～40cm, clip eugonic 3～the stem apex of 5cm, cut blade；
(4) stem apex cutting blade is carried out disinfection, during sterilization first with 70% soak with ethanol 30s, then use aseptic water washing
One time, then soak 8min with the mercuric chloride solution of 0.1%, re-use aseptic water washing four times；
(5) under anatomical lens, the stem apex after sterilization is stripped the growing point of the 0.2～0.3mm size with a piece of phyllopodium, connects
Plant in MS culture medium；
(6) postvaccinal culture dish be put into group training room in cultivate, cultivation temperature is 25 DEG C, intensity of illumination 2000～
3000LX, light application time 16h/d, when stem apex develops into the plantlet with 4～5 blades, by serum method, plantlet is entered
Row Viral diagnosis, removes and still carries virulent plant；
(7) in superclean bench, intercept the stem section of 4cm with the dissecting knife after sterilization, retain a piece of expansion on each stem section top
Leaf, inserts after removing the blade of bottom in the hole tray filling sterilization substrate；
(8) hole tray being plugged Seedling is put in booth, enclosed fly net, regular watering, nontoxic Beijing 553 Rhizoma Dioscoreae esculentae Seedling can be realized
Cultivation.
The method of a kind of Beijing 553 Sweetpotato Viruses Elimination nursery the most according to claim 1, it is characterised in that described step (1)
In passivation treatment method of virus be: first water-bath is warming up to 57 DEG C, Rhizoma Dioscoreae esculentae block soak 3min, then water temperature is down to 51
DEG C, Rhizoma Dioscoreae esculentae block is soaked 30min.
The method of a kind of Beijing 553 Sweetpotato Viruses Elimination nursery the most according to claim 1, it is characterised in that described step (2)
In sterilization medicinal liquid be dilution 50% carbendazim of 800 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610632538.3A CN106234220A (en) | 2016-08-03 | 2016-08-03 | A kind of method of Beijing 553 Sweetpotato Viruses Elimination nursery |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610632538.3A CN106234220A (en) | 2016-08-03 | 2016-08-03 | A kind of method of Beijing 553 Sweetpotato Viruses Elimination nursery |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106234220A true CN106234220A (en) | 2016-12-21 |
Family
ID=58077680
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610632538.3A Pending CN106234220A (en) | 2016-08-03 | 2016-08-03 | A kind of method of Beijing 553 Sweetpotato Viruses Elimination nursery |
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| CN (1) | CN106234220A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973716A (en) * | 2021-11-26 | 2022-01-28 | 广东海洋大学 | Tissue culture and rapid propagation method of sweet potatoes |
| CN116076367A (en) * | 2023-03-07 | 2023-05-09 | 山西农业大学玉米研究所(山西省农业科学院玉米研究所) | Device and method for peeling and cultivating detoxified seedlings from sweet potato stem tips |
-
2016
- 2016-08-03 CN CN201610632538.3A patent/CN106234220A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973716A (en) * | 2021-11-26 | 2022-01-28 | 广东海洋大学 | Tissue culture and rapid propagation method of sweet potatoes |
| CN116076367A (en) * | 2023-03-07 | 2023-05-09 | 山西农业大学玉米研究所(山西省农业科学院玉米研究所) | Device and method for peeling and cultivating detoxified seedlings from sweet potato stem tips |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20161221 |
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| WD01 | Invention patent application deemed withdrawn after publication |