CN106215010A - A kind of extracting method of Semen avenae nudae polyphenol - Google Patents
A kind of extracting method of Semen avenae nudae polyphenol Download PDFInfo
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- CN106215010A CN106215010A CN201610744750.9A CN201610744750A CN106215010A CN 106215010 A CN106215010 A CN 106215010A CN 201610744750 A CN201610744750 A CN 201610744750A CN 106215010 A CN106215010 A CN 106215010A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The extracting method of a kind of Semen avenae nudae polyphenol, relates to polyphenol extractive technique field, and it is completed by following steps: Semen avenae nudae bran → removal lipid → extraction free phenol → release combines phenol → extraction → rotary evaporation → combine phenol extraction thing；Therefore the beneficial effects of the present invention is: the present invention uses acid system to extract the combined state aldehydes matter in Semen avenae nudae, optimize test parameters, simplify operating procedure, obtain wide variety, rich content, and the Semen avenae nudae combined state aldehydes matter that antioxidant activity is high；This method operating procedure is relatively simple, and required time is short, and it is significantly different with content to there are some researches show that the plant using acid system and alkali process hydrolysis to obtain combines the kind of phenol, and the former total phenol content and oxidation resistance all high than the latter.
Description
One, technical field
The present invention relates to polyphenol extractive technique field, particularly relate to the extracting method of a kind of Semen avenae nudae polyphenol.
Two, background technology
Polyphenol substance is the compound that the class being widely present in nature contains multiple phenolic hydroxyl structure, mainly includes that class is yellow
The materials such as ketone, phenolic acid, tannin, have the strongest non-oxidizability and antiradical activities, can hinder oxide destruction lipid and low
Density lipoprotein, suppresses platelet aggregation, reduces coronary heart disease and the sickness rate of cancer, delays the effect such as tissue and human senility,
It it is good antioxidant；Vegetable and fruit are the sources of mankind's polyphenol substance, but more research shows, frumentum
Food is also the important sources of aldehydes matter.Modern popular disease research shows, edible kernel grains or Related product are to reduction
The chronic diseases such as cardiovascular and cerebrovascular vessel have good effect, the effect of the polyphenols being primarily due in kernel grains.
Polyphenol is also the intermediate product of secondary metabolism in corn body, mainly includes benzoic acid, cinnamic acid, anthocyanidin, quinone, Huang
Ketone, chalcone derivative, flavonol, flavone alkane and amino phenols acid compound and derivant thereof.Possibly together with tocotrienols, life in corn
Educate phenol, ferulic acid etc., and in vegetable and fruit, content is little.Polyphenol substance is typically with free state, esterification state and the shape of combined state
Formula is present in corn body, and wherein free state and esterification state are referred to as solubility phenols, and combined state is referred to as insoluble phenols, with
The forms such as ester bond, glycosidic bond, ether glycosidic bond combine with other materials (including protein, monosaccharide, organic acid etc.), many in corn
Phenol is most to be existed with combining form.These aldehydes matters can not be extracted by conventional extraction method, gives
Research in conjunction with phenol brings inconvenience, is even left in the basket；But these combine phenol and slowly can be digested by gastrointestinal tract until colon, same
Time discharge substantial amounts of polyphenol substance；Andreasen etc. confirm white mice faecal flora can by corn bran skin Ah
Wei, acid discharged；Adom etc. report, the polyphenol substance in corn has stronger oxidation resistance, and flavone also has potential
Oxidation resistance and anti-cancer ability.
Semen avenae nudae is the staple food crop of high and cold farming region, the Qinghai-Tibet Platean being harvested for one time each year, and is also that Qinghai-Tibet Platean is most characteristic
Crops, about 80% Tibetan compatriot (total population about 5,400,000) with Semen avenae nudae as staple food.As the big crop in little ancestor's grain, blue or green
The broad covered area of highland barley, total sown area about 380,000 hectares, account for more than 60% and total output of grain of Qinghai-Tibet Platean cereal crops area
58-60%.Containing multiple phenolic compound in Semen avenae nudae, such as materials such as tocol, flavone, anthocyanidin.And the phenolic material in Fructus Hordei Vulgaris
The content (0.2%-0.4%) of matter is far above other corn.But owing to the aldehydes matter in Fructus Hordei Vulgaris is difficult to separate, therefore, grope one
Plant the extracting method of aldehydes matter in Semen avenae nudae, the functional component composition to further investigation Semen avenae nudae, specify the anti-of Semen avenae nudae aldehydes matter
The aspects such as Oxidation are significant.
Extracting method currently, with respect to corn aldehydes matter is more, but is mostly only limitted to free state, and aldehydes matter is in paddy
Thing has free state, solubility combined state and 3 kinds of forms of insoluble combined state, and major part is all combined state；Wherein only trip
Amorph can be by organic solvent extraction, and combined state is generally combined with the macromolecular substances such as vegetable polysaccharides, protein with ehter bond and ester bond,
Can not be extracted by water or organic solvent；If the extraction only focusing on free phenol can be lost most combination phenol and be made it
Antioxidation declines to a great extent, therefore significant to the extraction of combined state aldehydes matter in Semen avenae nudae.
The extracting method combining phenol at present mainly has alkaline process, uses sodium hydroxide solution more, and acid system many employings hydrochloric acid,
Sulfuric acid solution, wherein alkaline process is the most commonly used, but Soviet Union and Eastern Europe find dawn the Fructus Litchi that acid system and alkali process hydrolysis obtain combine the kind of phenol with
Content is significantly different, and the former total binding phenol content and oxidation resistance all high than the latter；Arranze etc. with Semen Tritici aestivi are
Raw material extraction combines phenol and also obtains similar conclusion；Therefore, the Extraction solvent being badly in need of combining Semen avenae nudae phenol is determined, preparation technology
It is optimized and improves, it is achieved Semen avenae nudae combines efficiently separating of phenol, for the functional characteristic of thoroughly evaluating difference grain color Semen avenae nudae polyphenol
And application lays the foundation.
Three, summary of the invention
In order to overcome above-mentioned the deficiencies in the prior art, the invention provides the extracting method of a kind of Semen avenae nudae polyphenol: it is by following step
Suddenly complete: Semen avenae nudae bran → removal lipid → extraction free phenol → release combines phenol → extraction → rotary evaporation → combine phenol extraction
Thing；
The processing step removing lipid operation is: by standby Semen avenae nudae bran petroleum ether drip washing Semen avenae nudae bran 3～4 times, drip washing
Semen avenae nudae bran afterwards is standby；
The processing step extracting free phenol operation is: accurately weigh Semen avenae nudae bran 10 g standby after removing lipid, Semen avenae nudae bran
Carry out being mixed and stirred for uniformly, then under the conditions of 25 DEG C according to 1:20～25g/mL ratio with the acetone soln that concentration is 80%
By 400W ultrasonic Treatment 30min, then being centrifuged, the rotating speed of centrifuge is 4000r/min, and centrifugation time is 10min, has been centrifuged
Rear collection supernatant, is free phenol extract, and residue same method extracts twice, merges the supernatant collected, through 40～
45 DEG C of rotations are steamed to dry, extract absolute methanol solution is settled to 100mL, measures total phenol content with Forint phenol method, and residue is standby
With；
Release combines the processing step of phenol operation: above-mentioned standby residue joins the methanol sulfur that 17mL concentration is 10～12%
In acid solution, water-bath 1 hour under the conditions of 75 DEG C, do not regulate pH, solution for standby；
The processing step of extraction process is: adds 20mL anhydrous ethyl acetate in above-mentioned stock solution and extracts 5 times, obtains every time
Extract centrifuge take supernatant, the rotating speed of centrifuge is 2000-3000r/min, and centrifugation time is 5-8min, will
5 times the centrifugal supernatant obtained merges and standby；
Processing step in conjunction with phenol extraction thing operation is: by above-mentioned standby combined ethyl acetate extraction phase 40～45 DEG C of conditions
Lower rotary evaporated to dryness, residue methanol constant volume to 10mL, by constant volume solution with 0.45 μm organic membrane filter, obtain Semen avenae nudae and combine
State aldehydes matter extracting solution ,-20 DEG C keep in Dark Place；Mensuration for Forint phenol method total phenol content.
The beneficial effects of the present invention is: therefore the present invention uses acid system to carry the combined state aldehydes matter in Semen avenae nudae
Take, optimize test parameters, simplify operating procedure, obtained the Semen avenae nudae that wide variety, rich content, and antioxidant activity are high
Combined state aldehydes matter；This method operating procedure is relatively simple, and required time is short, and there are some researches show employing acid system and alkaline process water
The kind that the plant that solution obtains combines phenol is significantly different with content, and the former total phenol content and oxidation resistance all ratios rear
Person is high.
Four, detailed description of the invention
Embodiment 1
The extracting method of polyphenol in black highland barley, concrete operations are as follows:
Accurately weigh black highland barley wheat bran 10g, be the acetone soln that 1:25 ratio adds 250mL80% according to solid-liquid ratio, through the most mixed
Close uniformly, under the conditions of room temperature (25 DEG C), with 400W ultrasonic Treatment 30min, centrifugal (4000r/min, 10min), collect supernatant
Liquid.Residue same method extracts 2 times, merges 3 centrifugal supernatant, and 45 DEG C of rotations are steamed to dry, and residue is standby.Extract is used
Absolute methanol solution is settled to 100mL, and after measured, in black highland barley wheat bran, free polyphenol content is 273.94 mg/100g,
DPPH radical scavenging activity is 1897.95 μm ol/100g, and ferrous ion reducing power is 2246.10 μm ol/100g,
ABTS+radical scavenging activity is 5080.98 μm ol/100g.By above-mentioned residue add 17mL10%(mass concentration) first
Alcohol sulfuric acid solution, water-bath 1h at 75 DEG C, do not regulate pH.It is subsequently adding 20mL ethyl acetate to extract 5 times, centrifugal (3000r/min,
5min), combined ethyl acetate extraction phase.Extract is rotary evaporated to dryness under the conditions of 45 DEG C, and residue is by methanol constant volume extremely
10mL, 0.45 μm organic membrane filter, obtain Semen avenae nudae combined state aldehydes matter extracting solution ,-20 DEG C keep in Dark Place.Black highland barley bran after measured
Combination phenol content in skin is 187.74 mg/100g, and DPPH radical scavenging activity is 6197.30 μm ol/100g, ferrous
Ion reduction ability is 1288.13 μm ol/100g, and ABTS+radical scavenging activity is 1073.77 μm ol/100g.Pass through
The Semen avenae nudae polyphenol content that dissociates that the present embodiment method is extracted is higher than traditional water extract method by 77.62%, traditional in conjunction with phenol content ratio
Alkali extraction (sodium hydroxide solution) method is high by 47.32%.
Embodiment 2
The extracting method of purple Semen avenae nudae polyphenol, concrete operations are as follows:
Accurately weigh purple Semen avenae nudae bran 10g, be the acetone soln that 1:20 ratio adds 200mL80% according to solid-liquid ratio, through the most mixed
Close uniformly, under the conditions of room temperature (25 DEG C), with 400W ultrasonic Treatment 30min, centrifugal (4000r/min, 10min), collect supernatant
Liquid.Residue same method extracts 2 times, merges 3 centrifugal supernatant, and 40 DEG C of rotations are steamed to dry, and residue is standby.Extract is used
Absolute methanol solution is settled to 100mL, and after measured, in purple Semen avenae nudae bran, free polyphenol content is 235.96 mg/100g,
DPPH radical scavenging activity is 2284.47 μm ol/100g, and ferrous ion reducing power is 2041.16 μm ol/100g,
ABTS+radical scavenging activity is 5180.91 μm ol/100g.By above-mentioned residue add 17mL12%(mass concentration) first
Alcohol sulfuric acid solution, water-bath 1h at 75 DEG C, do not regulate pH.It is subsequently adding 20mL ethyl acetate to extract 5 times, centrifugal (2500r/min,
6min), combined ethyl acetate extraction phase.Extract is rotary evaporated to dryness under the conditions of 40 DEG C, and residue is by methanol constant volume extremely
10mL, 0.45 μm organic membrane filter, obtain Semen avenae nudae combined state aldehydes matter extracting solution ,-20 DEG C keep in Dark Place.Purple Semen avenae nudae bran after measured
Combination phenol content in skin is 172.97 mg/100g, and DPPH radical scavenging activity is 6142.77 μm ol/100g, ferrous
Ion reduction ability is 1266.95 μm ol/100g, and ABTS+radical scavenging activity is 1049.48 μm ol/100g.Pass through
The Semen avenae nudae polyphenol content that dissociates that the present embodiment method is extracted is higher than traditional water extract method by 46.77%, traditional in conjunction with phenol content ratio
Alkali extraction (sodium hydroxide solution) method is high by 60.02%.
Embodiment 3
The extracting method of blue Semen avenae nudae polyphenol, concrete operations are as follows:
Accurately weigh blue Semen avenae nudae bran 10g, be the acetone soln that 1:22 ratio adds 220mL80% according to solid-liquid ratio, through the most mixed
Close uniformly, under the conditions of room temperature (25 DEG C), with 100Hz ultrasonic Treatment 30min, centrifugal (4000r/min, 10min), collect supernatant
Liquid.Residue same method extracts 2 times, merges 3 centrifugal supernatant, and 42 DEG C of rotations are steamed to dry, and residue is standby.Extract is used
Absolute methanol solution is settled to 100mL, and after measured, in blue Semen avenae nudae bran, free polyphenol content is 209.09 mg/100g,
DPPH radical scavenging activity is 1469.50 μm ol/100g, and ferrous ion reducing power is 1858.78 μm ol/100g,
ABTS+radical scavenging activity is 4068.20 μm ol/100g.By above-mentioned residue add 17mL11%(mass concentration) first
Alcohol sulfuric acid solution, water-bath 1h at 75 DEG C, do not regulate pH.It is subsequently adding 20mL ethyl acetate to extract 5 times, centrifugal (2000r/min,
8min), combined ethyl acetate extraction phase.Extract is rotary evaporated to dryness under the conditions of 42 DEG C, and residue is by methanol constant volume extremely
10mL, 0.45 μm organic membrane filter, obtain Semen avenae nudae combined state aldehydes matter extracting solution ,-20 DEG C keep in Dark Place.Blue Semen avenae nudae bran after measured
Combination phenol content in skin is 216.41 mg/100g, and DPPH radical scavenging activity is 6334.14 μm ol/100g, ferrous
Ion reduction ability 1603.50 μm ol/100g, ABTS+radical scavenging activity is 1222.38 μm ol/100g.By this
The Semen avenae nudae polyphenol content that dissociates that embodiment method is extracted is higher than traditional water extract method by 68.71%, in conjunction with phenol content than traditional alkali
Extraction (sodium hydroxide solution) method is high by 49.03%.
Embodiment 4
The extracting method of white Semen avenae nudae polyphenol, concrete operations are as follows:
Accurately weigh white Semen avenae nudae bran 10g, be the acetone soln that 1:20 ratio adds 200mL80% according to solid-liquid ratio, through the most mixed
Close uniformly, under the conditions of room temperature (25 DEG C), with 100Hz ultrasonic Treatment 30min, centrifugal (4000r/min, 10min), collect supernatant
Liquid.Residue same method extracts 2 times, merges 3 centrifugal supernatant, and 45 DEG C of rotations are steamed to dry, and residue is standby.Extract is used
Absolute methanol solution is settled to 100mL, and after measured, in white Semen avenae nudae bran, free polyphenol content is 269.06 mg/100g,
DPPH radical scavenging activity is 1951.04 μm ol/100g, and ferrous ion reducing power is 2286.04 μm ol/100g,
ABTS+radical scavenging activity is 4935.80 μm ol/100g.By above-mentioned residue add 17mL12%(mass concentration) first
Alcohol sulfuric acid solution, water-bath 1h at 75 DEG C, do not regulate pH.It is subsequently adding 20mL ethyl acetate to extract 5 times, centrifugal (2000r/min,
8min), combined ethyl acetate extraction phase.Extract is rotary evaporated to dryness under the conditions of 45 DEG C, and residue is by methanol constant volume extremely
10mL, 0.45 μm organic membrane filter, obtain Semen avenae nudae combined state aldehydes matter extracting solution ,-20 DEG C keep in Dark Place.White Semen avenae nudae bran after measured
Combination phenol content in skin is 233.89 mg/100g, and DPPH radical scavenging activity is 6547.60 μm ol/100g, ferrous
Ion reduction ability is 1540.01 μm ol/100g, and ABTS+radical scavenging activity is 1117.71 μm ol/100g.Pass through
The Semen avenae nudae polyphenol content that dissociates that the present embodiment method is extracted is higher than traditional water extract method by 67.98%, traditional in conjunction with phenol content ratio
Alkali extraction (sodium hydroxide solution) method is high by 54.67%.
Embodiment 5
The extracting method of a kind of Semen avenae nudae polyphenol: it is completed by following steps: Semen avenae nudae bran → removal lipid → extraction free phenol →
Release combines phenol → extraction → rotary evaporation → combine phenol extraction thing；
The processing step removing lipid operation is: by standby Semen avenae nudae bran petroleum ether drip washing Semen avenae nudae bran 3 times, after drip washing
Semen avenae nudae bran standby；
The processing step extracting free phenol operation is: accurately weigh Semen avenae nudae bran 10 g standby after removing lipid, Semen avenae nudae bran
Carry out being mixed and stirred for uniformly, then using under the conditions of 25 DEG C according to 1:20g/mL ratio with the acetone soln that concentration is 80%
100Hz ultrasonic Treatment 30min, then be centrifuged, the rotating speed of centrifuge is 4000r/min, and centrifugation time is 10min, has been centrifuged
Rear collection supernatant, is free phenol extract, and residue same method extracts twice, merges the supernatant collected, through 40 DEG C
Rotation is steamed to dry, extract absolute methanol solution is settled to 100mL, measures total phenol content with Forint phenol method, and residue is standby；
Release combines the processing step of phenol operation: above-mentioned standby residue is joined the methanol sulphuric acid that 17mL concentration is 10% molten
In liquid, water-bath 1 hour under the conditions of 75 DEG C, do not regulate pH, solution for standby；
The processing step of extraction process is: adds 20mL anhydrous ethyl acetate in above-mentioned stock solution and extracts 5 times, obtains every time
Extract centrifuge take supernatant, the rotating speed of centrifuge is 3000r/min, and centrifugation time is 5min, by 5 times be centrifuged
The supernatant obtained merges and standby；
Processing step in conjunction with phenol extraction thing operation is: by above-mentioned standby combined ethyl acetate extraction phase at 40 DEG C of condition backspins
Turn and be evaporated to dryness, residue methanol constant volume to 10mL, by constant volume solution with 0.45 μm organic membrane filter, obtain Semen avenae nudae combined state phenol
Class material extracting solution ,-20 DEG C keep in Dark Place；Mensuration for Forint phenol method total phenol content.
Embodiment 6
The extracting method of a kind of Semen avenae nudae polyphenol: it is completed by following steps: Semen avenae nudae bran → removal lipid → extraction free phenol →
Release combines phenol → extraction → rotary evaporation → combine phenol extraction thing；
The processing step removing lipid operation is: by standby Semen avenae nudae bran petroleum ether drip washing Semen avenae nudae bran 3～4 times, drip washing
Semen avenae nudae bran afterwards is standby；
The processing step extracting free phenol operation is: accurately weigh Semen avenae nudae bran 10 g standby after removing lipid, Semen avenae nudae bran
Carry out being mixed and stirred for uniformly, then using under the conditions of 25 DEG C according to 1:25g/mL ratio with the acetone soln that concentration is 80%
100Hz ultrasonic Treatment 30min, then be centrifuged, the rotating speed of centrifuge is 4000r/min, and centrifugation time is 10min, has been centrifuged
Rear collection supernatant, is free phenol extract, and residue same method extracts twice, merges the supernatant collected, through 45 DEG C
Rotation is steamed to dry, extract absolute methanol solution is settled to 100mL, measures total phenol content with Forint phenol method, and residue is standby；
Release combines the processing step of phenol operation: above-mentioned standby residue is joined the methanol sulphuric acid that 17mL concentration is 12% molten
In liquid, water-bath 1 hour under the conditions of 75 DEG C, do not regulate pH, solution for standby；
The processing step of extraction process is: adds 20mL anhydrous ethyl acetate in above-mentioned stock solution and extracts 5 times, obtains every time
Extract centrifuge take supernatant, the rotating speed of centrifuge is 2000r/min, and centrifugation time is 8min, by 5 times be centrifuged
The supernatant obtained merges and standby；
Processing step in conjunction with phenol extraction thing operation is: by above-mentioned standby combined ethyl acetate extraction phase at 45 DEG C of condition backspins
Turn and be evaporated to dryness, residue methanol constant volume to 10mL, by constant volume solution with 0.45 μm organic membrane filter, obtain Semen avenae nudae combined state phenol
Class material extracting solution ,-20 DEG C keep in Dark Place；Mensuration for Forint phenol method total phenol content.
Claims (1)
1. the extracting method of a Semen avenae nudae polyphenol: it is characterized in that: it is completed by following steps: Semen avenae nudae bran → removal lipid →
Extract free phenol → release and combine phenol → extraction → rotary evaporation → combine phenol extraction thing；
The processing step removing lipid operation is: by standby Semen avenae nudae bran petroleum ether drip washing Semen avenae nudae bran 3～4 times, drip washing
Semen avenae nudae bran afterwards is standby；
The processing step extracting free phenol operation is: accurately weigh Semen avenae nudae bran 10 g standby after removing lipid, Semen avenae nudae bran
Carry out being mixed and stirred for uniformly, then under the conditions of 25 DEG C according to 1:20～25g/mL ratio with the acetone soln that concentration is 80%
By 400W ultrasonic Treatment 30min, then being centrifuged, the rotating speed of centrifuge is 4000r/min, and centrifugation time is 10min, has been centrifuged
Rear collection supernatant, is free phenol extract, and residue same method extracts twice, merges the supernatant collected, through 40～
45 DEG C of rotations are steamed to dry, extract absolute methanol solution is settled to 100mL, measures total phenol content with Forint phenol method, and residue is standby
With；
Release combines the processing step of phenol operation: above-mentioned standby residue joins the methanol sulfur that 17mL concentration is 10～12%
In acid solution, water-bath 1 hour under the conditions of 75 DEG C, do not regulate pH, solution for standby；
The processing step of extraction process is: adds 20mL anhydrous ethyl acetate in above-mentioned stock solution and extracts 5 times, obtains every time
Extract centrifuge take supernatant, the rotating speed of centrifuge is 2000～3000r/min, and centrifugation time is 5～8min,
5 centrifugal supernatant obtained are merged and standby；
Processing step in conjunction with phenol extraction thing operation is: by above-mentioned standby combined ethyl acetate extraction phase 40～45 DEG C of conditions
Lower rotary evaporated to dryness, residue methanol constant volume to 10mL, by constant volume solution with 0.45 μm organic membrane filter, obtain Semen avenae nudae and combine
State aldehydes matter extracting solution ,-20 DEG C keep in Dark Place；Mensuration for Forint phenol method total phenol content.
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Cited By (7)
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| CN105125742A (en) * | 2015-08-25 | 2015-12-09 | 华侨大学 | Method of extracting combination type phenolic compounds from camellia sinensis seed oil |
| CN107242567A (en) * | 2017-06-15 | 2017-10-13 | 西南大学 | A kind of preparation method of balck vein hickory chick meals polyphenol |
| CN109805406A (en) * | 2019-02-19 | 2019-05-28 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of extracting method of rice bran combination phenol super-pressure-alkali-sour water solution substep release |
| CN111067033A (en) * | 2020-01-06 | 2020-04-28 | 青海大学 | Highland barley food rich in soluble phenols and preparation method thereof |
| CN113244657A (en) * | 2021-06-18 | 2021-08-13 | 四川省农业科学院农产品加工研究所 | Echelon extraction method of functional components of purple highland barley bran |
| CN113730951A (en) * | 2021-08-02 | 2021-12-03 | 江苏大学 | Subcritical water extraction method for improving antioxidant activity of sorghum bran combined-state polyphenol |
| CN116223412A (en) * | 2023-02-13 | 2023-06-06 | 西藏自治区农牧科学院农业研究所 | Method for detecting content of phenols in highland barley |
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