CN116223412A - Method for detecting content of phenols in highland barley - Google Patents
Method for detecting content of phenols in highland barley Download PDFInfo
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- CN116223412A CN116223412A CN202310103232.9A CN202310103232A CN116223412A CN 116223412 A CN116223412 A CN 116223412A CN 202310103232 A CN202310103232 A CN 202310103232A CN 116223412 A CN116223412 A CN 116223412A
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- highland barley
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- 235000007340 Hordeum vulgare Nutrition 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 11
- 150000002989 phenols Chemical class 0.000 title description 4
- 240000005979 Hordeum vulgare Species 0.000 title 1
- 241000209219 Hordeum Species 0.000 claims abstract description 27
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 18
- 239000012488 sample solution Substances 0.000 claims abstract description 18
- 239000000523 sample Substances 0.000 claims abstract description 15
- 239000000126 substance Substances 0.000 claims abstract description 14
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 12
- 239000003960 organic solvent Substances 0.000 claims abstract description 11
- 238000007873 sieving Methods 0.000 claims abstract description 11
- 239000012498 ultrapure water Substances 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000013339 cereals Nutrition 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 238000002835 absorbance Methods 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims description 5
- 238000001514 detection method Methods 0.000 abstract description 7
- 238000000605 extraction Methods 0.000 abstract description 2
- 230000031700 light absorption Effects 0.000 abstract 1
- YQUVCSBJEUQKSH-UHFFFAOYSA-N protochatechuic acid Natural products OC(=O)C1=CC=C(O)C(O)=C1 YQUVCSBJEUQKSH-UHFFFAOYSA-N 0.000 description 3
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 3
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 2
- 235000001785 ferulic acid Nutrition 0.000 description 2
- 229940114124 ferulic acid Drugs 0.000 description 2
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 2
- PCMORTLOPMLEFB-ONEGZZNKSA-N sinapic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC(OC)=C1O PCMORTLOPMLEFB-ONEGZZNKSA-N 0.000 description 2
- JMSVCTWVEWCHDZ-UHFFFAOYSA-N syringic acid Chemical compound COC1=CC(C(O)=O)=CC(OC)=C1O JMSVCTWVEWCHDZ-UHFFFAOYSA-N 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- QBLFZIBJXUQVRF-UHFFFAOYSA-N (4-bromophenyl)boronic acid Chemical compound OB(O)C1=CC=C(Br)C=C1 QBLFZIBJXUQVRF-UHFFFAOYSA-N 0.000 description 1
- WXTMDXOMEHJXQO-UHFFFAOYSA-N 2,5-dihydroxybenzoic acid Chemical compound OC(=O)C1=CC(O)=CC=C1O WXTMDXOMEHJXQO-UHFFFAOYSA-N 0.000 description 1
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 1
- QURCVMIEKCOAJU-HWKANZROSA-N Isoferulic acid Natural products COC1=CC=C(\C=C\C(O)=O)C=C1O QURCVMIEKCOAJU-HWKANZROSA-N 0.000 description 1
- 108010028554 LDL Cholesterol Proteins 0.000 description 1
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 1
- 235000001368 chlorogenic acid Nutrition 0.000 description 1
- 229940074393 chlorogenic acid Drugs 0.000 description 1
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- -1 polyphenol compounds Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- PCMORTLOPMLEFB-UHFFFAOYSA-N sinapinic acid Natural products COC1=CC(C=CC(O)=O)=CC(OC)=C1O PCMORTLOPMLEFB-UHFFFAOYSA-N 0.000 description 1
- YIBXWXOYFGZLRU-UHFFFAOYSA-N syringic aldehyde Natural products CC12CCC(C3(CCC(=O)C(C)(C)C3CC=3)C)C=3C1(C)CCC2C1COC(C)(C)C(O)C(O)C1 YIBXWXOYFGZLRU-UHFFFAOYSA-N 0.000 description 1
- WKOLLVMJNQIZCI-UHFFFAOYSA-N vanillic acid Chemical compound COC1=CC(C(O)=O)=CC=C1O WKOLLVMJNQIZCI-UHFFFAOYSA-N 0.000 description 1
- TUUBOHWZSQXCSW-UHFFFAOYSA-N vanillic acid Natural products COC1=CC(O)=CC(C(O)=O)=C1 TUUBOHWZSQXCSW-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4055—Concentrating samples by solubility techniques
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4055—Concentrating samples by solubility techniques
- G01N2001/4061—Solvent extraction
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a method for detecting the content of phenolic substances in highland barley, which comprises the following steps: crushing highland barley grains, sieving to obtain crushed aggregates, taking 3 sample bottles, quantitatively adding crushed aggregates into the sample bottles, adding 100 milliliters of organic solvent into each bottle, respectively adding 5 grams, 10 grams and 15 grams of crushed aggregates into each bottle, centrifuging for 10 minutes at the environment of the rotating speed of 2000 revolutions per minute at the environment of 25 ℃, filtering the centrifuged solution, carrying out water bath for 3 hours at the water bath temperature of 40 ℃, obtaining an extract by an ultrasonic extractor, adding high-purity water to a constant volume, preparing a sample solution, and measuring the light absorption value of the highland barley sample solution by an ultraviolet-visible photometer so as to determine the polyphenol substance content in the sample solution. The invention has the advantages that: the polyphenol substances in the highland barley can be separated out by means of water bath, extraction and the like, so that the detection is more convenient.
Description
Technical Field
The invention relates to the technical field of phenolic substance detection, in particular to a method for detecting the content of phenolic substances in highland barley.
Background
The highland barley seed contains various phenolic compounds, mainly ferulic acid, isoferulic acid, vanillic acid, coumaric acid, syringic acid, hydroxybenzoic acid, dihydroxybenzoic acid, sinapic acid, chlorogenic acid, protocatechuic acid, catechin, isocatechuic acid, etc. In highland barley, the ferulic acid content is highest, and is about 68% of the total phenol content. The polyphenol compounds are highly correlated with the antioxidant activity of highland barley, and can effectively reduce the content of low-density lipoprotein cholesterol and the atherosclerosis index. At present, researches on the content of polyphenol in highland barley by scientific researchers are few, a perfect method for detecting the content of the polyphenol in highland barley is not established, and at present, crushed highland barley crushed aggregates are not thoroughly treated, so that the measurement accuracy of the content of the polyphenol is affected.
Disclosure of Invention
The invention provides a method for detecting the content of phenolic substances in highland barley in order to solve the problems.
In order to solve the technical problems, the technical scheme provided by the invention is as follows: a method for detecting the content of phenols in highland barley comprises the following steps:
step one: crushing highland barley grains, and sieving to obtain crushed materials;
step two: taking 3 sample bottles, quantitatively adding crushed aggregates into the sample bottles, adding 100 milliliters of organic solvent into each bottle, wherein the crushed aggregates added into each bottle are 5 grams, 10 grams and 15 grams respectively;
step three: centrifuging the solid-liquid mixture in the second step for 10 minutes at the rotating speed of 2000 revolutions per minute and the environment temperature of 25 ℃;
step four: filtering the solution after the centrifugation in the step three, and carrying out water bath for 3 hours at 40 ℃;
step five: obtaining an extract by an ultrasonic extractor, adding high-purity water to fix the volume, and preparing a sample solution;
step six: and measuring the absorbance value of the highland barley sample solution by using an ultraviolet-visible spectrophotometer so as to determine the polyphenol substance content in the sample solution.
Preferably, the sieving treatment in the first step adopts a 90-mesh sieve.
Preferably, the organic solvent in the second step is an ethanol solution.
Preferably, in the fifth step, the high-purity water is kept stand for 10 to 15 minutes after the volume is fixed.
Compared with the prior art, the invention has the advantages that: the detection method can accurately measure the content of the polyphenol substances in the highland barley, and the polyphenol substances in the highland barley can be separated out by means of water bath, extraction and the like, so that the detection is more convenient.
Detailed Description
The present invention will be described in further detail with reference to examples.
Example 1
Step one: crushing highland barley grains, and sieving to obtain crushed materials;
step two: taking 3 sample bottles, quantitatively adding crushed aggregates into the sample bottles, adding 100 milliliters of organic solvent into each bottle, respectively taking 5 grams, 10 grams and 15 grams of crushed aggregates added into each bottle, and taking 5 grams of the sample bottles for detection;
step three: centrifuging the solid-liquid mixture in the second step for 10 minutes at the rotating speed of 2000 revolutions per minute and the environment temperature of 25 ℃;
step four: filtering the solution after the centrifugation in the step three, and carrying out water bath for 3 hours at 40 ℃;
step five: obtaining an extract by an ultrasonic extractor, adding high-purity water to fix the volume, and preparing a sample solution;
step six: and measuring the absorbance value of the highland barley sample solution by using an ultraviolet-visible spectrophotometer so as to determine the polyphenol substance content in the sample solution.
The sieving treatment in the first step adopts a 90-mesh sieve.
The organic solvent in the second step is ethanol solution.
In the fifth step, the high-purity water is kept stand for 10 to 15 minutes after the volume is fixed.
Example 2
Step one: crushing highland barley grains, and sieving to obtain crushed materials;
step two: taking 3 sample bottles, quantitatively adding crushed aggregates into the sample bottles, adding 100 milliliters of organic solvent into each bottle, respectively taking 5 grams, 10 grams and 15 grams of crushed aggregates added into each bottle, and taking 10 grams of the sample bottles for detection;
step three: centrifuging the solid-liquid mixture in the second step for 10 minutes at the rotating speed of 2000 revolutions per minute and the environment temperature of 25 ℃;
step four: filtering the solution after the centrifugation in the step three, and carrying out water bath for 3 hours at 40 ℃;
step five: obtaining an extract by an ultrasonic extractor, adding high-purity water to fix the volume, and preparing a sample solution;
step six: and measuring the absorbance value of the highland barley sample solution by using an ultraviolet-visible spectrophotometer so as to determine the polyphenol substance content in the sample solution.
The sieving treatment in the first step adopts a 90-mesh sieve.
The organic solvent in the second step is ethanol solution.
In the fifth step, the high-purity water is kept stand for 10 to 15 minutes after the volume is fixed.
Example 3
Step one: crushing highland barley grains, and sieving to obtain crushed materials;
step two: taking 3 sample bottles, quantitatively adding crushed aggregates into the sample bottles, adding 100 milliliters of organic solvent into each bottle, respectively taking 5 grams, 10 grams and 15 grams of crushed aggregates added into each bottle, and taking 5 grams of the sample bottles for detection;
step three: centrifuging the solid-liquid mixture in the second step for 15 minutes at the rotating speed of 2000 revolutions per minute and the environment temperature of 25 ℃;
step four: filtering the solution after the centrifugation in the step three, and carrying out water bath for 3 hours at 40 ℃;
step five: obtaining an extract by an ultrasonic extractor, adding high-purity water to fix the volume, and preparing a sample solution;
step six: and measuring the absorbance value of the highland barley sample solution by using an ultraviolet-visible spectrophotometer so as to determine the polyphenol substance content in the sample solution.
The sieving treatment in the first step adopts a 90-mesh sieve.
The organic solvent in the second step is ethanol solution.
In the fifth step, the high-purity water is kept stand for 10 to 15 minutes after the volume is fixed.
The invention and its embodiments have been described above without limitation, and the actual construction is not limited thereto. In summary, if one of ordinary skill in the art is informed by this disclosure, a structural manner and an embodiment similar to the technical solution should not be creatively devised without departing from the gist of the present invention.
Claims (4)
1. The method for detecting the content of the phenolic substances in the highland barley is characterized by comprising the following steps of:
step one: crushing highland barley grains, and sieving to obtain crushed materials;
step two: taking 3 sample bottles, quantitatively adding crushed aggregates into the sample bottles, adding 100 milliliters of organic solvent into each bottle, wherein the crushed aggregates added into each bottle are 5 grams, 10 grams and 15 grams respectively;
step three: centrifuging the solid-liquid mixture in the second step for 10 minutes at the rotating speed of 2000 revolutions per minute and the environment temperature of 25 ℃;
step four: filtering the solution after the centrifugation in the step three, and carrying out water bath for 3 hours at 40 ℃;
step five: obtaining an extract by an ultrasonic extractor, adding high-purity water to fix the volume, and preparing a sample solution;
step six: and measuring the absorbance value of the highland barley sample solution by using an ultraviolet-visible spectrophotometer so as to determine the polyphenol substance content in the sample solution.
2. A method as claimed in claim 1, wherein: the sieving treatment in the first step adopts a 90-mesh sieve.
3. A method as claimed in claim 1, wherein: the organic solvent in the second step is ethanol solution.
4. A method as claimed in claim 1, wherein: in the fifth step, the high-purity water is kept stand for 10 to 15 minutes after the volume is fixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310103232.9A CN116223412A (en) | 2023-02-13 | 2023-02-13 | Method for detecting content of phenols in highland barley |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310103232.9A CN116223412A (en) | 2023-02-13 | 2023-02-13 | Method for detecting content of phenols in highland barley |
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| CN116223412A true CN116223412A (en) | 2023-06-06 |
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|---|---|---|---|
| CN202310103232.9A Pending CN116223412A (en) | 2023-02-13 | 2023-02-13 | Method for detecting content of phenols in highland barley |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103558214A (en) * | 2013-11-04 | 2014-02-05 | 北京林业大学 | Method for measuring content of polyphenols in fargesia denudate |
| CN106215010A (en) * | 2016-08-29 | 2016-12-14 | 青海大学 | A kind of extracting method of Semen avenae nudae polyphenol |
| CN109966773A (en) * | 2017-11-22 | 2019-07-05 | 大江生医股份有限公司 | The method of active constituent is extracted from object to be extracted |
| CN114848692A (en) * | 2022-04-27 | 2022-08-05 | 天津农学院 | Extraction process and application of quinoa seedling polyphenol substances |
-
2023
- 2023-02-13 CN CN202310103232.9A patent/CN116223412A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103558214A (en) * | 2013-11-04 | 2014-02-05 | 北京林业大学 | Method for measuring content of polyphenols in fargesia denudate |
| CN106215010A (en) * | 2016-08-29 | 2016-12-14 | 青海大学 | A kind of extracting method of Semen avenae nudae polyphenol |
| CN109966773A (en) * | 2017-11-22 | 2019-07-05 | 大江生医股份有限公司 | The method of active constituent is extracted from object to be extracted |
| CN114848692A (en) * | 2022-04-27 | 2022-08-05 | 天津农学院 | Extraction process and application of quinoa seedling polyphenol substances |
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Application publication date: 20230606 |