CN106212875A - Lactogenesis granule and preparation method thereof - Google Patents

Lactogenesis granule and preparation method thereof Download PDF

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Publication number
CN106212875A
CN106212875A CN201610599821.0A CN201610599821A CN106212875A CN 106212875 A CN106212875 A CN 106212875A CN 201610599821 A CN201610599821 A CN 201610599821A CN 106212875 A CN106212875 A CN 106212875A
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chinese medicine
parts
medicine extract
lactogenesis
filtrate
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宗敏玲
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To Be Grown Up In Jiangxi Pharmaceutcal Corp Ltd
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To Be Grown Up In Jiangxi Pharmaceutcal Corp Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/906—Zingiberaceae (Ginger family)
    • A61K36/9064—Amomum, e.g. round cardamom
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232—Angelica
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/236—Ligusticum (licorice-root)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284—Atractylodes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/34—Campanulaceae (Bellflower family)
    • A61K36/344—Codonopsis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A61K36/638—Ligustrum, e.g. Chinese privet
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/894—Dioscoreaceae (Yam family)
    • A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8969—Polygonatum (Solomon's seal)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

Abstract

The invention discloses a kind of lactogenesis granule and preparation method thereof, Chinese medicine extract in raw material uses particular extraction procedure to propose effective ingredient, add natural plant composition again, part material is carried out pretreatment, the raw material of pretreatment can strengthen the effect of Chinese medicine extract and natural plant composition, the lactogenesis granule prepared is possible not only to fill blood poultry, lactogenesis, and has certain prevention effect to hepatitis.

Description

Lactogenesis granule and preparation method thereof
Technical field
The present invention relates to a kind of lactogenesis granule and preparation method thereof, belong to field of animal feed.
Background technology
Feedstuff, is the general name of the food of everyone domesticated animal, compares narrowly general feeds and generally refers to agricultural Or the food of animal husbandry domesticated animal.Feedstuff include Semen sojae atricolor, bean cake, Semen Maydis, fish flour, aminoacid, the miscellaneous dregs of rice, additive, whey powder, The feedstuff of more than ten kinds such as oils and fats, meat meal tankage, corn, sugar grass.
Animal feed is all directly to add various Chinese medicines, DeGrain at present.
Summary of the invention
It is an object of the invention to provide a kind of lactogenesis granule and preparation method thereof, the Chinese medicine extract in raw material uses specific Extracting method proposes effective ingredient, then adds natural plant composition, and part material is carried out pretreatment, and the raw material of pretreatment is permissible Strengthening Chinese medicine extract and the effect of natural plant composition, prepared lactogenesis granule is possible not only to fill blood poultry, lactogenesis, and And hepatitis is had certain prevention effect.
A kind of lactogenesis granule, is made up of the raw material of following masses number: Chinese medicine extract 900-1000 part, vitamin C 4.5-5.5 part, vitamin E 1.5-2.5 part.
A kind of lactogenesis granule, is made up of the raw material of following masses number: Chinese medicine extract 900-1000 part, vitamin C 4.5-5.5 part, vitamin E 1.5-2.5 part, Rhizoma Polygonati Odorati 87-89 part, Fructus Amomi 55-57 part, lysine 13-17 part, cobalt carbonate 8-10 Part, manganese sulfate 17-19 part, glycine betaine 9-11 part, dimethyl fumarate 7-8 part, Butylated hydroxyanisole 5-7 part, bentonite 10- 11 parts, white carbon 6-7 part.
Described Chinese medicine extract is made up of the raw material of following masses number: Radix Codonopsis 34-36 part, Radix Astragali 29-31 part, when Return 26-27 part, Rhizoma Atractylodis Macrocephalae 21-23 part, Radix Glycyrrhizae 15-17 part, Rhizoma Chuanxiong 18-20 part, Fructus Ligustri Lucidi 9-10 part, Rhizoma Dioscoreae 9-10 part.
The preparation method of Chinese medicine extract is:
(1) take the Rhizoma Atractylodis Macrocephalae to pulverize, add 95% soak with ethanol 24 hours of 11-13 times of quality, filter, obtain leachate；
(2) leachate concentrating under reduced pressure recycling design to without alcohol taste, extractum is obtained, extractum by 1.4-1.6 times of extractum quality Distilled water miscible, obtain miscible fluid；
(3) the miscible fluid ethyl acetate of 1/2 miscible fluid volume is extracted, isolated water layer and ethyl acetate layer；
(4) HD-8 ion exchange resin column on ethyl acetate layer, upper column flow rate is 1.5-1.7BV/h, successively with 2.5BV 10% ethanol, 30% ethanol of 2BV, 50% ethanol of 1.5BV, 70% ethanol of 1BV, 90% ethanol of 0.5BV, carry out ladder Degree eluting, elution flow rate is 1.5-1.7BV/h, collects gradient eluent；
(5) Rhizoma Chuanxiong is pulverized, decoct 100-140 minute with the distilled water of 15-17 times of quality, filter, obtain filtrate；
(6) the n-butanol extraction of filtrate equal volume, isolated water layer and n-butanol layer；
(7) Radix Codonopsis, Radix Angelicae Sinensis, Rhizoma Dioscoreae are pulverized respectively, in the water layer that addition to (3) step obtains, 70-80 DEG C of heating extraction 5-6 hour, filter, obtain filtrate I；
(8) Radix Astragali, Radix Glycyrrhizae, Fructus Ligustri Lucidi are pulverized respectively, add the water layer obtained to (6) step, 60-70 DEG C of heating extraction 8-9 hour, filter, obtain filtrate II；
(9) filtrate I and filtrate II are merged, be evaporated to the 2/3 of volume, be spray-dried, obtain Chinese medicine extract.
Wherein, in step (3), the volume ratio of ethyl acetate layer and HD-8 ion exchange resin is 2:1, the height of resin column With diameter than for 8:1.
A kind of lactogenesis granule, its preparation method is:
(1) take Rhizoma Polygonati Odorati to pulverize, by 60-70 DEG C of heating and refluxing extraction 140-160 minute of methanol of 19-21 times of quality, filter, Obtain extracting solution；
(2) extracting solution decompression and solvent recovery to without alcohol taste, extractum is obtained；
(3) Fructus Amomi is pulverized, extract 180-220 minute with distilled water 80-90 DEG C of 21-23 times of quality, filter, obtain filter Liquid；
(4) extractum that (2) step is obtained is dissolved in the filtrate of 3 () step, obtains miscible fluid, then uses miscible fluid equal-volume Chloroform extraction, isolated water layer；
(5) water layer is evaporated to the 1/2 of original volume, is spray-dried, obtains pretreated raw material；
(6) Chinese medicine extract taking 1/6 mass is placed in mixer, adds pretreated raw material, vitamin C, dimension life Element E, lysine, cobalt carbonate, manganese sulfate, glycine betaine, dimethyl fumarate, Butylated hydroxyanisole, bentonite, white carbon, mixed Close 20-30 minute, standby；
(7) taking remaining Chinese medicine extract to be placed in another mixer, add step (6) gained material, mixing 20-30 divides Clock, subpackage, to obtain final product.
The solution have the advantages that:
1, during Radix Codonopsis, the Radix Astragali, Radix Angelicae Sinensis, the Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae, Rhizoma Chuanxiong, Fructus Ligustri Lucidi and Rhizoma Dioscoreae use specific preparation method to obtain Medicament extract, effectively extracted fill blood, raw composition of milk, effect is better than being provided without extracting method and other extracting method Radix Codonopsis, the Radix Astragali, Radix Angelicae Sinensis, the Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae, Rhizoma Chuanxiong, Fructus Ligustri Lucidi and Rhizoma Dioscoreae.
2, raw material uses Rhizoma Polygonati Odorati and the Fructus Amomi of plant component, containing natural constituent, has no side effect.
3, preparation process first carries out pretreatment the Rhizoma Polygonati Odorati in raw material and Fructus Amomi, then make lactogenesis with other raw materials Grain.
4, the raw material of the pretreatment of the present invention can strengthen the effect of Chinese medicine extract and natural plant composition, San Zhelian With, the lactogenesis granule prepared is possible not only to fill blood poultry, lactogenesis, and has certain prevention effect to hepatitis.
Detailed description of the invention
Below by embodiment, the invention will be further described.
Embodiment 1:
A kind of lactogenesis granule, is made up of the raw material of following masses number: Chinese medicine extract 900 parts, vitamin C 4.5 parts, Vitamin E 1.5 parts, Rhizoma Polygonati Odorati 87 parts, Fructus Amomi 55 parts, lysine 13 parts, cobalt carbonate 8 parts, manganese sulfate 17 parts, glycine betaine 9 parts, richness Horse dimethyl phthalate 7 parts, Butylated hydroxyanisole 5 parts, bentonite 10 parts, white carbon 6 parts.
Described Chinese medicine extract is made up of the raw material of following masses number: Radix Codonopsis 34 parts, the Radix Astragali 29 parts, Radix Angelicae Sinensis 26 Part, the Rhizoma Atractylodis Macrocephalae 21 parts, 15 parts of Radix Glycyrrhizae, Rhizoma Chuanxiong 18 parts, Fructus Ligustri Lucidi 9 parts, Rhizoma Dioscoreae 9 parts.
The preparation method of Chinese medicine extract is:
(1) take the Rhizoma Atractylodis Macrocephalae to pulverize, add 95% soak with ethanol 24 hours of 11 times of quality, filter, obtain leachate；
(2) leachate concentrating under reduced pressure recycling design to without alcohol taste, extractum is obtained, the extractum steaming of 1.4 times of extractum quality Distilled water is miscible, obtains miscible fluid；
(3) the miscible fluid ethyl acetate of 1/2 miscible fluid volume is extracted, isolated water layer and ethyl acetate layer；
(4) HD-8 ion exchange resin column on ethyl acetate layer, upper column flow rate is 1.5BV/h, successively with 2.5BV's 10% ethanol, 30% ethanol of 2BV, 50% ethanol of 1.5BV, 70% ethanol of 1BV, 90% ethanol of 0.5BV, carry out gradient Eluting, elution flow rate is 1.5BV/h, collects gradient eluent；
(5) Rhizoma Chuanxiong is pulverized, decoct 100 minutes with the distilled water of 15 times of quality, filter, obtain filtrate；
(6) the n-butanol extraction of filtrate equal volume, isolated water layer and n-butanol layer；
(7) Radix Codonopsis, Radix Angelicae Sinensis, Rhizoma Dioscoreae are pulverized respectively, in the water layer that addition to (3) step obtains, 70-80 DEG C of heating extraction 5-6 hour, filter, obtain filtrate I；
(8) Radix Astragali, Radix Glycyrrhizae, Fructus Ligustri Lucidi being pulverized respectively, add the water layer obtained to (6) step, 60 DEG C of heating extraction 8 are little Time, filter, obtain filtrate II；
(9) filtrate I and filtrate II are merged, be evaporated to the 2/3 of volume, be spray-dried, obtain Chinese medicine extract.
Wherein, in step (3), the volume ratio of ethyl acetate layer and HD-8 ion exchange resin is 2:1, the height of resin column With diameter than for 8:1.
A kind of lactogenesis granule, its preparation method is:
(1) take Rhizoma Polygonati Odorati to pulverize, with 60 DEG C of heating and refluxing extraction of methanol 140 minutes of 19 times of quality, filtration, extracted Liquid；
(2) extracting solution decompression and solvent recovery to without alcohol taste, extractum is obtained；
(3) Fructus Amomi is pulverized, extract 180 minutes with the distilled water 80 DEG C of 21 times of quality, filter, obtain filtrate；
(4) extractum that (2) step is obtained is dissolved in the filtrate of 3 () step, obtains miscible fluid, then uses miscible fluid equal-volume Chloroform extraction, isolated water layer；
(5) water layer is evaporated to the 1/2 of original volume, is spray-dried, obtains pretreated raw material；
(6) Chinese medicine extract taking 1/6 mass is placed in mixer, adds pretreated raw material, vitamin C, dimension life Element E, lysine, cobalt carbonate, manganese sulfate, glycine betaine, dimethyl fumarate, Butylated hydroxyanisole, bentonite, white carbon, mixed Close 20 minutes, standby；
(7) take remaining Chinese medicine extract to be placed in another mixer, add step (6) gained material, mix 20 minutes, Subpackage, to obtain final product.
Embodiment 2:
A kind of lactogenesis granule, is made up of the raw material of following masses number: Chinese medicine extract 950 parts, vitamin C 5 parts, dimension Raw element E 2 parts, Rhizoma Polygonati Odorati 878 parts, Fructus Amomi 56 parts, lysine 15 parts, cobalt carbonate 9 parts, manganese sulfate 18 parts, glycine betaine 10 parts, rich horse Dimethyl phthalate 7.5 parts, Butylated hydroxyanisole 6 parts, bentonite 10.5 parts, white carbon 6.5 parts.
Described Chinese medicine extract is made up of the raw material of following masses number: Radix Codonopsis 35 parts, the Radix Astragali 30 parts, Radix Angelicae Sinensis 26.5 Part, the Rhizoma Atractylodis Macrocephalae 22 parts, 16 parts of Radix Glycyrrhizae, Rhizoma Chuanxiong 19 parts, Fructus Ligustri Lucidi 9.5 parts, Rhizoma Dioscoreae 9.5 parts.
The preparation method of Chinese medicine extract is:
(1) take the Rhizoma Atractylodis Macrocephalae to pulverize, add 95% soak with ethanol 24 hours of 12 times of quality, filter, obtain leachate；
(2) leachate concentrating under reduced pressure recycling design to without alcohol taste, extractum is obtained, the extractum steaming of 1.5 times of extractum quality Distilled water is miscible, obtains miscible fluid；
(3) the miscible fluid ethyl acetate of 1/2 miscible fluid volume is extracted, isolated water layer and ethyl acetate layer；
(4) HD-8 ion exchange resin column on ethyl acetate layer, upper column flow rate is 1.6BV/h, successively with 2.5BV's 10% ethanol, 30% ethanol of 2BV, 50% ethanol of 1.5BV, 70% ethanol of 1BV, 90% ethanol of 0.5BV, carry out gradient Eluting, elution flow rate is 1.6BV/h, collects gradient eluent；
(5) Rhizoma Chuanxiong is pulverized, decoct 120 minutes with the distilled water of 16 times of quality, filter, obtain filtrate；
(6) the n-butanol extraction of filtrate equal volume, isolated water layer and n-butanol layer；
(7) Radix Codonopsis, Radix Angelicae Sinensis, Rhizoma Dioscoreae are pulverized respectively, in the water layer that addition to (3) step obtains, 75 DEG C of heating extraction 5.5 Hour, filter, obtain filtrate I；
(8) Radix Astragali, Radix Glycyrrhizae, Fructus Ligustri Lucidi are pulverized respectively, add the water layer obtained to (6) step, 65 DEG C of heating extraction 8.5 Hour, filter, obtain filtrate II；
(9) filtrate I and filtrate II are merged, be evaporated to the 2/3 of volume, be spray-dried, obtain Chinese medicine extract.
Wherein, in step (3), the volume ratio of ethyl acetate layer and HD-8 ion exchange resin is 2:1, the height of resin column With diameter than for 8:1.
A kind of lactogenesis granule, its preparation method is:
(1) take Rhizoma Polygonati Odorati to pulverize, with 65 DEG C of heating and refluxing extraction of methanol 150 minutes of 20 times of quality, filtration, extracted Liquid；
(2) extracting solution decompression and solvent recovery to without alcohol taste, extractum is obtained；
(3) Fructus Amomi is pulverized, extract 200 minutes with the distilled water 85 DEG C of 22 times of quality, filter, obtain filtrate；
(4) extractum that (2) step is obtained is dissolved in the filtrate of 3 () step, obtains miscible fluid, then uses miscible fluid equal-volume Chloroform extraction, isolated water layer；
(5) water layer is evaporated to the 1/2 of original volume, is spray-dried, obtains pretreated raw material；
(6) Chinese medicine extract taking 1/6 mass is placed in mixer, adds pretreated raw material, vitamin C, dimension life Element E, lysine, cobalt carbonate, manganese sulfate, glycine betaine, dimethyl fumarate, Butylated hydroxyanisole, bentonite, white carbon, mixed Close 25 minutes, standby；
(7) take remaining Chinese medicine extract to be placed in another mixer, add step (6) gained material, mix 25 minutes, Subpackage, to obtain final product.
Embodiment 3:
A kind of lactogenesis granule, is made up of the raw material of following masses number: Chinese medicine extract 1000 parts, vitamin C 5.5 parts, Vitamin E 2.5 parts, Rhizoma Polygonati Odorati 89 parts, Fructus Amomi 57 parts, lysine 17 parts, cobalt carbonate 10 parts, manganese sulfate 19 parts, glycine betaine 11 parts, Dimethyl fumarate 8 parts, Butylated hydroxyanisole 7 parts, bentonite 11 parts, white carbon 7 parts.
Described Chinese medicine extract is made up of the raw material of following masses number: Radix Codonopsis 36 parts, the Radix Astragali 31 parts, Radix Angelicae Sinensis 27 Part, the Rhizoma Atractylodis Macrocephalae 23 parts, 17 parts of Radix Glycyrrhizae, Rhizoma Chuanxiong 20 parts, Fructus Ligustri Lucidi 10 parts, Rhizoma Dioscoreae 10 parts.
The preparation method of Chinese medicine extract is:
(1) take the Rhizoma Atractylodis Macrocephalae to pulverize, add 95% soak with ethanol 24 hours of 13 times of quality, filter, obtain leachate；
(2) leachate concentrating under reduced pressure recycling design to without alcohol taste, extractum is obtained, the extractum steaming of 1.6 times of extractum quality Distilled water is miscible, obtains miscible fluid；
(3) the miscible fluid ethyl acetate of 1/2 miscible fluid volume is extracted, isolated water layer and ethyl acetate layer；
(4) HD-8 ion exchange resin column on ethyl acetate layer, upper column flow rate is 1.7BV/h, successively with 2.5BV's 10% ethanol, 30% ethanol of 2BV, 50% ethanol of 1.5BV, 70% ethanol of 1BV, 90% ethanol of 0.5BV, carry out gradient Eluting, elution flow rate is 1.7BV/h, collects gradient eluent；
(5) Rhizoma Chuanxiong is pulverized, decoct 140 minutes with the distilled water of 17 times of quality, filter, obtain filtrate；
(6) the n-butanol extraction of filtrate equal volume, isolated water layer and n-butanol layer；
(7) Radix Codonopsis, Radix Angelicae Sinensis, Rhizoma Dioscoreae being pulverized respectively, in the water layer that addition to (3) step obtains, 80 DEG C of heating extraction 6 are little Time, filter, obtain filtrate I；
(8) Radix Astragali, Radix Glycyrrhizae, Fructus Ligustri Lucidi being pulverized respectively, add the water layer obtained to (6) step, 70 DEG C of heating extraction 9 are little Time, filter, obtain filtrate II；
(9) filtrate I and filtrate II are merged, be evaporated to the 2/3 of volume, be spray-dried, obtain Chinese medicine extract.
Wherein, in step (3), the volume ratio of ethyl acetate layer and HD-8 ion exchange resin is 2:1, the height of resin column With diameter than for 8:1.
A kind of lactogenesis granule, its preparation method is:
(1) take Rhizoma Polygonati Odorati to pulverize, with 70 DEG C of heating and refluxing extraction of methanol 160 minutes of 21 times of quality, filtration, extracted Liquid；
(2) extracting solution decompression and solvent recovery to without alcohol taste, extractum is obtained；
(3) Fructus Amomi is pulverized, extract 220 minutes with the distilled water 90 DEG C of 23 times of quality, filter, obtain filtrate；
(4) extractum that (2) step is obtained is dissolved in the filtrate of 3 () step, obtains miscible fluid, then uses miscible fluid equal-volume Chloroform extraction, isolated water layer；
(5) water layer is evaporated to the 1/2 of original volume, is spray-dried, obtains pretreated raw material；
(6) Chinese medicine extract taking 1/6 mass is placed in mixer, adds pretreated raw material, vitamin C, dimension life Element E, lysine, cobalt carbonate, manganese sulfate, glycine betaine, dimethyl fumarate, Butylated hydroxyanisole, bentonite, white carbon, mixed Close 30 minutes, standby；
(7) take remaining Chinese medicine extract to be placed in another mixer, add step (6) gained material, mix 30 minutes, Subpackage, to obtain final product.
Comparative example 1:
A kind of lactogenesis granule, is made up of the raw material of following masses number: Chinese medicine extract 1200 parts, vitamin C 3 parts, dimension Raw element E 5 parts, Rhizoma Polygonati Odorati 85 parts, Fructus Amomi 60 parts, lysine 10 parts, cobalt carbonate 15 parts, manganese sulfate 15 parts, glycine betaine 7 parts, fumaric acid Dimethyl ester 10 parts, Butylated hydroxyanisole 3 parts, bentonite 15 parts, white carbon 5 parts.
Described Chinese medicine extract is made up of the raw material of following masses number: Radix Codonopsis 30 parts, the Radix Astragali 35 parts, Radix Angelicae Sinensis 25 Part, the Rhizoma Atractylodis Macrocephalae 25 parts, 13 parts of Radix Glycyrrhizae, Rhizoma Chuanxiong 25 parts, Fructus Ligustri Lucidi 7 parts, Rhizoma Dioscoreae 15 parts.
Remaining is with embodiment 1.
Comparative example 2:
A kind of lactogenesis granule, the preparation method of Chinese medicine extract is:
(1) take the Rhizoma Atractylodis Macrocephalae to pulverize, add 75% soak with ethanol 24 hours of 12 times of quality, filter, obtain leachate；
(2) leachate concentrating under reduced pressure recycling design to without alcohol taste, extractum is obtained, the extractum steaming of 1.5 times of extractum quality Distilled water is miscible, obtains miscible fluid；
(3) the miscible fluid ethyl acetate of 1/2 miscible fluid volume is extracted, isolated water layer and ethyl acetate layer；
(4) D001 ion exchange resin column on ethyl acetate layer, upper column flow rate is 1.6BV/h, successively with 2.5BV's 10% methanol, 30% methanol of 2BV, 50% methanol of 1.5BV, 70% methanol of 1BV, 90% methanol of 0.5BV, carry out gradient Eluting, elution flow rate is 1.6BV/h, collects gradient eluent；
(5) Rhizoma Chuanxiong is pulverized, decoct 120 minutes with the distilled water of 16 times of quality, filter, obtain filtrate；
(6) the n-butanol extraction of filtrate equal volume, isolated water layer and n-butanol layer；
(7) Radix Codonopsis, Radix Angelicae Sinensis, Rhizoma Dioscoreae are pulverized respectively, in the water layer that addition to (3) step obtains, 75 DEG C of heating extraction 5.5 Hour, filter, obtain filtrate I；
(8) Radix Astragali, Radix Glycyrrhizae, Fructus Ligustri Lucidi are pulverized respectively, add the water layer obtained to (6) step, 65 DEG C of heating extraction 8.5 Hour, filter, obtain filtrate II；
(9) filtrate I and filtrate II are merged, be evaporated to the 2/3 of volume, be spray-dried, obtain Chinese medicine extract.
Wherein, in step (3), the volume ratio of ethyl acetate layer and D001 ion exchange resin is 2:1, the height of resin column With diameter than for 8:1.
Remaining is with embodiment 2.
Comparative example 3:
A kind of lactogenesis granule, is made up of the raw material of following masses number: Chinese medicine extract 1000 parts, vitamin C 5.5 parts, Vitamin E2 .5 part, lysine 17 parts, cobalt carbonate 10 parts, manganese sulfate 19 parts, glycine betaine 11 parts, dimethyl fumarate 8 parts, butyl BHA 7 parts, bentonite 11 parts, white carbon 7 parts.
A kind of lactogenesis granule, its preparation method is:
(1) Chinese medicine extract taking 1/6 mass is placed in mixer, adds vitamin C, vitamin E, lysine, carbonic acid Cobalt, manganese sulfate, glycine betaine, dimethyl fumarate, Butylated hydroxyanisole, bentonite, white carbon, mix 20-30 minute, standby With；
(2) taking remaining Chinese medicine extract to be placed in another mixer, add step (1) gained material, mixing 20-30 divides Clock, subpackage, to obtain final product.
Remaining is with embodiment 3.
1. the present invention experiment to galgctogogue action
Select SD pregnant rats 70, be randomly divided into 7 groups: matched group, embodiment 1-3 group and comparative example 1-3 group, often organize 10 Only.0.9% normal saline of matched group oral administration gavage 20ml/kg, embodiment 1-3 group and comparative example 1-3 group are respectively by embodiment The feedstuff oral administration gavage 2g/kg that 1-3 group and comparative example 1-3 group prepare.Often group retains puerperal filial mice 10.After childbirth the Within three days, carry out lactogenic test.
Weigh each weight organizing filial mice before and after sucking breast milk respectively.The breast that this group filial mice averagely sucks is tried to achieve with its difference Amount.Ask to be 40 minutes when sucking.After record administration, (i.e. filial mice averagely sucks the dams galactopoiesis amount of the first, the 3rd, the 5th day Breast amount).The results are shown in Table 1.
Table 1 present invention impact on rat milk secretory volume
Group First day (g) 3rd day (g) 5th day (g)
Matched group 0.65±0.23 2.76±0.31 2.97±0.63
Embodiment 1 0.81±0.62 4.09±0.58 6.52±1.49
Embodiment 2 0.97±0.65 4.35±0.64 6.91±1.57
Embodiment 3 0.88±0.59 4.12±0.62 6.78±1.48
Comparative example 1 0.75±0.31 3.50±0.53 4.53±1.21
Comparative example 2 0.70±0.46 3.28±0.57 3.75±0.86
Comparative example 3 0.72±0.38 3.31±0.49 3.96±0.90
As known from Table 1, the lactogenesis granule (embodiment 1-3) that the present invention prepares can increase the secretory volume of rat milk, and And effect is substantially better than comparative example 1 (proportioning raw materials is different) and comparative example 2 (extraction process of Chinese medicine extract is different), comparative example 3 (being not added with Rhizoma Polygonati Odorati and Fructus Amomi in raw material), especially optimum with the effect of embodiment 2, it is seen that proportioning raw materials, Chinese medicine extract The curative effect of prepared product is all had an impact by extraction process and natural plant composition, therefore present invention employs specific raw material and joins Than, the extraction process of Chinese medicine extract with select suitable natural plant composition, prepared lactogenesis granule has galgctogogue action, hence it is evident that Improve lactogenesis amount.
2. the prevention effect of hepatitis is demonstrated by the present invention
Take 70 mices, be randomly divided into 7 groups: matched group, embodiment 1-3 group and comparative example 1-3 group, often group 10, each group Animal fasting overnight 16h, often gavage of group gives 1%CCl4Vegetable oil solution 5ml/kg, each embodiment 1-3 group and right after 4h The feedstuff 2g/kg that ratio 1-3 group gavage embodiment 1-3 group respectively and comparative example 1-3 group prepare, after 24h, each group mouse orbit takes Blood, separates serum, takes rapidly liver, and filter paper blots, and claims liver quality, calculates liver coefficient (liver mass/body weight).Result such as table 2 Shown in.
Table 2 present invention effect to mouse liver
As known from Table 2, the lactogenesis granule (embodiment 1-3) that the present invention prepares can substantially recover the liver matter of mice Amount, liver coefficient and GPT value, and effect is better than comparative example 1 (proportioning raw materials is different) and the comparative example 2 (extraction of Chinese medicine extract Technique is different), comparative example 3 (being not added with Rhizoma Polygonati Odorati and Fructus Amomi 5 in raw material), the especially effect with embodiment 2 optimum, it is seen that raw material The curative effect of prepared product is all had an impact by proportioning, the extraction process of Chinese medicine extract and natural plant composition, the therefore present invention Have employed specific proportioning raw materials, the extraction process of Chinese medicine extract and select suitable natural plant composition, prepared lactogenesis Granule has certain prevention effect to hepatitis.

Claims (5)

1. a lactogenesis granule, is characterized by: be made up of the raw material of following masses number: Chinese medicine extract 900-1000 part, dimension Raw element C 4.5-5.5 part, vitamin E 1.5-2.5 part.
2. a lactogenesis granule, is characterized by: be made up of the raw material of following masses number: Chinese medicine extract 900-1000 part, dimension Raw element C 4.5-5.5 part, vitamin E 1.5-2.5 part, Rhizoma Polygonati Odorati 87-89 part, Fructus Amomi 55-57 part, lysine 13-17 part, carbonic acid Cobalt 8-10 part, manganese sulfate 17-19 part, glycine betaine 9-11 part, dimethyl fumarate 7-8 part, Butylated hydroxyanisole 5-7 part, swollen Profit soil 10-11 part, white carbon 6-7 part.
3. a kind of lactogenesis granule as claimed in claim 2, is characterized by: Chinese medicine extract is by the raw material system of following masses number Become: Radix Codonopsis 34-36 part, Radix Astragali 29-31 part, Radix Angelicae Sinensis 26-27 part, Rhizoma Atractylodis Macrocephalae 21-23 part, Radix Glycyrrhizae 15-17 part, Rhizoma Chuanxiong 18-20 part, female Loyal sub-9-10 part, Rhizoma Dioscoreae 9-10 part.
4. a kind of lactogenesis granule, is characterized by:
The preparation method of Chinese medicine extract is:
(1) take the Rhizoma Atractylodis Macrocephalae to pulverize, add 95% soak with ethanol 24 hours of 11-13 times of quality, filter, obtain leachate；
(2) leachate concentrating under reduced pressure recycling design to without alcohol taste, extractum is obtained, the extractum steaming of 1.4-1.6 times of extractum quality Distilled water is miscible, obtains miscible fluid；
(3) the miscible fluid ethyl acetate of 1/2 miscible fluid volume is extracted, isolated water layer and ethyl acetate layer；
(4) HD-8 ion exchange resin column on ethyl acetate layer, upper column flow rate is 1.5-1.7BV/h, successively with 2.5BV's 10% ethanol, 30% ethanol of 2BV, 50% ethanol of 1.5BV, 70% ethanol of 1BV, 90% ethanol of 0.5BV, carry out gradient Eluting, elution flow rate is 1.5-1.7BV/h, collects gradient eluent；
(5) Rhizoma Chuanxiong is pulverized, decoct 100-140 minute with the distilled water of 15-17 times of quality, filter, obtain filtrate；
(6) the n-butanol extraction of filtrate equal volume, isolated water layer and n-butanol layer；
(7) Radix Codonopsis, Radix Angelicae Sinensis, Rhizoma Dioscoreae are pulverized respectively, in the water layer that addition to (3) step obtains, 70-80 DEG C of heating extraction 5-6 Hour, filter, obtain filtrate I；
(8) Radix Astragali, Radix Glycyrrhizae, Fructus Ligustri Lucidi are pulverized respectively, add the water layer obtained to (6) step, 60-70 DEG C of heating extraction 8-9 Hour, filter, obtain filtrate II；
(9) filtrate I and filtrate II are merged, be evaporated to the 2/3 of volume, be spray-dried, obtain Chinese medicine extract；
Wherein, in step (3), the volume ratio of ethyl acetate layer and HD-8 ion exchange resin is 2:1, and the height of resin column is with straight Footpath is than for 8:1.
5. a kind of lactogenesis granule as claimed in claim 2, is characterized by:
Its preparation method is:
(1) take Rhizoma Polygonati Odorati to pulverize, by 60-70 DEG C of heating and refluxing extraction 140-160 minute of methanol of 19-21 times of quality, filter, obtain Extracting solution；
(2) extracting solution decompression and solvent recovery to without alcohol taste, extractum is obtained；
(3) Fructus Amomi is pulverized, extract 180-220 minute with distilled water 80-90 DEG C of 21-23 times of quality, filter, obtain filtrate；
(4) extractum that (2) step is obtained is dissolved in the filtrate of 3 () step, obtains miscible fluid, then with the isopyknic chlorine of miscible fluid Imitative extraction, isolated water layer；
(5) water layer is evaporated to the 1/2 of original volume, is spray-dried, obtains pretreated raw material；
(6) Chinese medicine extract taking 1/6 mass is placed in mixer, add pretreated raw material, vitamin C, vitamin E, Lysine, cobalt carbonate, manganese sulfate, glycine betaine, dimethyl fumarate, Butylated hydroxyanisole, bentonite, white carbon, mix 20- 30 minutes, standby；
(7) take remaining Chinese medicine extract to be placed in another mixer, add step (6) gained material, mix 20-30 minute, Subpackage, to obtain final product.
CN201610599821.0A 2016-07-27 2016-07-27 Lactogenesis granule and preparation method thereof Pending CN106212875A (en)

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Application publication date: 20161214