CN106212274A - The method for tissue culture of special beautiful Caulis et folium euphorbiae milii - Google Patents
The method for tissue culture of special beautiful Caulis et folium euphorbiae milii Download PDFInfo
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- CN106212274A CN106212274A CN201610559183.XA CN201610559183A CN106212274A CN 106212274 A CN106212274 A CN 106212274A CN 201610559183 A CN201610559183 A CN 201610559183A CN 106212274 A CN106212274 A CN 106212274A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses the method for tissue culture of special beautiful Caulis et folium euphorbiae milii, including choosing outer implant, initial culture, successive transfer culture, root culture, transplanting；To clean, after sterilization, special beautiful Caulis et folium euphorbiae milii current-year branch is outer implant, is inoculated into bud inducement culture medium, carries out initial culture and is then seeded into successive transfer culture in proliferated culture medium, then inoculating and carry out root culture in root media, Seedling of finally taking root carries out transplanting and obtains regeneration plant.The method using the present invention, rooting rate is high, survival rate is high, reproduction speed is fast, the spy's beautiful Caulis et folium euphorbiae milii robust growth obtained, and has wide market prospect.
Description
Technical field
The invention belongs to the propagation method of plant, particularly to the method for tissue culture of special beautiful Caulis et folium euphorbiae milii.
Background technology
Special beautiful Caulis et folium euphorbiae milii, has another name called lucky fruit, red century, red lantern, ocean red, golden century in winter etc., is the Rosaceae of North America introduction
Malus griggles, female parent is Malus baccata, and male parent is not quite clear.Defoliation small arbor, bark is smooth, and blade is glossy；Pollen is red in vain
Color, flower amount is big, and the florescence is long, generally more than 20 days；Mid or late April result, mid or late October fruit maturation, peel is red, fruit
Neatly, uniformly, young fruit is green, and the later stage transfers redness to, and result quantities is big, arranges successively on branch, and ability of being annual fruiting is strong, bears fruit
Phase is long, can be suspended to the Second Year January through processing.This kind has stronger thermostability, winter resistance, drought resistance and disease-resistant
Property, shade tree can be done, be also to see one of fine work seeds in fruit bowl scape, be to see flower, see leaf, see the fine tree species of fruit.
Special beautiful Caulis et folium euphorbiae milii seminal propagation cycle length, seed are few, use propagation by grafiting now more, but due to by season, scion
Number quantitative limitation, it is impossible to carry out large-scale breeding, thus limit the popularization and application of this kind.Chinese invention patent (authorizes public affairs
Announcement CN 103392597 B authorized announcement date 2015.12.23) disclose the method for tissue culture of a kind of North American begonia, pass through
Initial culture, successive transfer culture, root culture and acclimatization and transplants, giving birth to shoot then with North American begonia is outer implant, through aseptic
After being inoculated on initial culture base cultivation a period of time, after being cut by the North American begonia seedling on initial culture base, it is seeded in subculture
Carry out successive transfer culture in culture medium, be transferred on root media carry out root induction when tufted seedling grows to 2～3cm and form life
Root, Seedling of taking root obtains regeneration plant through seedling exercising domestication, and the method for this invention makes the breeding of North American begonia the easiest, short
Obtaining substantial amounts of aseptic seedling in time, the breeding cycle shortens, but all adds NAA (1-naphthalene second in this invention in each culture medium
Acid), NAA has toxicity to human body, and the tufted seedling being transferred on root media is 2～3cm, the tufted seedling mistake of 2～3cm
Little, access and need longer incubation time to grow root system on root media.It addition, North American begonia is by non-refractory weather not
Can in megathermal climate area large-scale promotion, and a kind-Te Li Caulis et folium euphorbiae milii in North American begonia is at megathermal climate area energy
Enough well adapt to weather naturally grow.
Summary of the invention
The purpose of the present invention is aiming at the deficiency of above technology, it is provided that a kind of reproduction speed is fast, medium component low toxicity
Property, the method for tissue culture of the beautiful Caulis et folium euphorbiae milii of spy that survival rate is high.
The technical scheme is that
1, the method for tissue culture of special beautiful Caulis et folium euphorbiae milii, comprises the following steps:
1) outer implant is chosen: on special beautiful Caulis et folium euphorbiae milii, cut the current-year branch containing axillalry bud, enter over cleaning, in nothing after sterilization
Current-year branch is cut by bacterium operating board the stem section containing an axillalry bud, as outer implant；
2) initial culture: outer implant is accessed bud inducement culture medium, carries out initial culture, treat to grow at the axillalry bud of outer implant
Multiple Buds；
3) successive transfer culture: isolate 1.6～2cm single twigs in Multiple Buds, proceeds to cultivate in proliferated culture medium, treats
The base portion of twig grows the Multiple Buds of 3.1～3.5cm；The upgrowth situation of the twig of 1.6～2cm is preferable, it is easy at enrichment culture
Cultivating in base, the Multiple Buds of 3.1～3.5cm is suitably carrying out root culture, and the least meeting of Multiple Buds chosen causes rootage duration
Oversize, Multiple Buds too conference causes root system rare.
4) root culture: Multiple Buds is separated into single twig again, twig is accessed in root media and takes root
Cultivate 48～52 days, form Seedling of taking root；
5) transplant: by taking root in root media, Seedling is placed 1～2 day in natural light, then transplants, and transplanting medium is
Ratio of weight and number, the Vermiculitum peat composed of rotten mosses: leaf mould is 1:1～2:1～2.Seedling of taking root is positioned to make Seedling of taking root in natural light
Slowly adapting to the environment of outside, Seedling of taking root after contributing to transplanting grows faster, and transplanting medium is the growth according to special beautiful Caulis et folium euphorbiae milii
Needing, the substrate of preparation has preferable breathability, it is to avoid rotten of special beautiful Caulis et folium euphorbiae milii, the peat composed of rotten mosses, have in leaf mould abundant organic matter,
Humic acid and a small amount of vitamin, auxin, trace element etc., can promote the growth promoter of plant.
Wherein, the formula of bud inducement culture medium is: the PVP of NAA, 5.0g/L of 6-BA, 0.2mg/L of MS, 1.0mg/L,
The sucrose of 30g/L, the agar of 5.4g/L, pH 5.2～5.5；
The formula of proliferated culture medium is: the sucrose of IBA, 30g/L of 6-BA, 0.2mg/L of MS, 1.0mg/L, 5.4g/L
Agar, pH 5.2～5.5；
The formula of root media is: the sucrose of IBA, 30g/L of 1/2MS, 0.2mg/L, the agar of 5.4g/L, pH 5.2
～5.5.
Wherein, MS culture medium is minimal medium, and adding sucrose provides carbon source, adds agar preparation solid medium, respectively
Planting culture medium is to add 6-BA (6-benzyl aminopurine, purchased from Sigma), PVP (polyvinyl pyrrole on the basis of minimal medium
Alkanone, purchased from Sigma), add PVP and can slow down Brown, IBA (indolebutyric acid, purchased from Sigma), NAA (1-naphthalene second
Acid, purchased from Sigma).NAA all it is not added with at proliferated culture medium and root media, because NAA has certain toxicity to people, and
And show that in successive transfer culture with process of rooting culture NAA is not the biggest to the effect of the growth of seedling through many experiments.
Described step 1) in sterilization method be: the current-year branch after cleaning up is immersed in the ethanol postincubation of 75%
20～40 seconds, then with aseptic water washing current-year branch 3～5 times, rinse 1～3 minute every time, then be immersed in the chlorine of 0.1%
Change and hydrargyrum processes 8～12 minutes, the most again with aseptic water washing 3～5 times, rinse 1～3 minute every time.Use ethanol and chlorination
The sterilization method that hydrargyrum combines, bacteria-eliminating efficacy is good, and the damage that current-year branch is subject to is less.
The temperature of described initial culture is 24～26 DEG C, and light application time is every day 14～16h, intensity of illumination be 1800～
2000Lx；The temperature of described successive transfer culture is 24～26 DEG C, and light application time is every day 16～18h, intensity of illumination be 2000～
2200Lx；The temperature of described root culture is 24～26 DEG C, and light application time is every day 12～14h, intensity of illumination be 2200～
2400Lx.The differentiation of light application time and the intensity propagation and organ to cultivating cell has a major impact, and intensity of illumination is relatively strong, seedling
Grow is sturdy, and intensity of illumination more weak growth of seedling excessive growth, therefore the intensity of illumination in initial culture with successive transfer culture is relatively
Weak, beneficially Multiple Buds growth, and in root culture, intensity of illumination is relatively big, beneficially seedling takes root, and grows sturdy.At initial culture
Longer with the light application time in successive transfer culture, because the photoreaction that early stage Multiple Buds needs are carried out is to store substantial amounts of energy, and
In root culture, light application time is shorter, because twig needs dark reaction to promote the growth of root, stem and leaf.
Described transplanting processes the MS nutritional solution spraying dilution 1～10 times the same day on blade face, then sprays on every circumference blade face
Spill the MS nutritional solution of dilution 1～10 times, spray 4～6 times altogether.During transplanting, Seedling of taking root is in fast-growth period, needs substantial amounts of
Nutrient substance is supplied.MS nutritional solution could spray after needing dilution, and the highest meeting of concentration causes burn seedlings.
Preferably, described step 1) in special beautiful Caulis et folium euphorbiae milii be 4～the beautiful Caulis et folium euphorbiae milii of spy in May, 4～the beautiful Caulis et folium euphorbiae milii of spy in May be in one
Growing the most vigorous period in Nian, taking from the branch in this period, to carry out tissue culture's rooting rate as outer implant higher.
Beneficial effects of the present invention:
1, the breeding of special beautiful Caulis et folium euphorbiae milii realizes the anniversary, limited by the time with propagation by grafiting compared with, the present invention can be in group
Training room realizes anniversary breeding, factorial praluction.
2, the spy's beautiful Caulis et folium euphorbiae milii tissue cultured seedling rooting rate using the method for tissue culture of the present invention to obtain is high, survival rate is high, breeding
Speed is fast, and breeding coefficient reaches more than 4.2, and rooting rate reaches more than 90%, the spy's beautiful Caulis et folium euphorbiae milii robust growth obtained, and has wide
Market prospect.
Detailed description of the invention
Embodiment 1
1, the method for tissue culture of special beautiful Caulis et folium euphorbiae milii, including:
1) outer implant is chosen: on the beautiful Caulis et folium euphorbiae milii of spy in April, cut the current-year branch containing axillalry bud, enter over cleaning, disappear
Current-year branch is cut the stem section containing an axillalry bud, as outer implant after poison in aseptic operating platform；The method of sterilization is: will
Current-year branch after cleaning up is immersed in the ethanol postincubation 20 seconds of 75%, then with aseptic water washing current-year branch 3 times,
Rinse 3 minutes every time, then be immersed in the mercuric chloride of 0.1% process 8 minutes, use aseptic water washing 3 times the most again, rinse every time
3 minutes.
2) initial culture: outer implant is accessed bud inducement culture medium, carries out initial culture, treat to grow at the axillalry bud of outer implant
Multiple Buds；The formula of bud inducement culture medium is: PVP, 30g/L's of NAA, 5.0g/L of 6-BA, 0.2mg/L of MS, 1.0mg/L
Sucrose, the agar of 5.4g/L, pH 5.2；The temperature of initial culture is 24 DEG C, and light application time is 14h every day, light intensity 1800Lx；
3) successive transfer culture: isolate the single twig of 1.6cm in Multiple Buds, proceeds to cultivate in proliferated culture medium, treats tender
The base portion of branch grows the Multiple Buds of 3.1cm；The formula of proliferated culture medium is: the IBA of 6-BA, 0.2mg/L of MS, 1.0mg/L,
The sucrose of 30g/L, the agar of 5.4g/L, pH 5.2；The temperature of successive transfer culture is 24 DEG C, and light application time is 16h every day, light intensity
2000Lx；
4) root culture: Multiple Buds is separated into single twig again, twig is accessed in root media and takes root
Cultivate 48 days, form Seedling of taking root；The formula of root media is: the sucrose of IBA, 30g/L of 1/2MS, 0.2mg/L, 5.4g/L
Agar, pH 5.2；The temperature of root culture is 24 DEG C, and light application time is 12h every day, light intensity 2200Lx.
5) transplant: by Seedling of taking root in root media at natural light, ambient temperatare is put 1 day, then transplants, transplanting medium
For ratio of weight and number, the Vermiculitum peat composed of rotten mosses: leaf mould is 1:1:1.Transplanting processes the MS nutrition spraying dilution one times the same day on blade face
Liquid, then sprays the MS nutritional solution of dilution one times, sprays 4 times altogether on every circumference blade face.
Embodiment 2
1, the method for tissue culture of special beautiful Caulis et folium euphorbiae milii, including:
1) outer implant is chosen: on the beautiful Caulis et folium euphorbiae milii of spy in May, cut the current-year branch containing axillalry bud, enter over cleaning, disappear
Current-year branch is cut the stem section containing an axillalry bud, as outer implant after poison in aseptic operating platform；The method of sterilization is: will
Current-year branch after cleaning up is immersed in the ethanol postincubation 40 seconds of 75%, then with aseptic water washing current-year branch 5 times,
Rinse 1 minute every time, then be immersed in the mercuric chloride of 0.1% process 12 minutes, use aseptic water washing 5 times the most again, rush every time
Wash 1 minute.
2) initial culture: outer implant is accessed bud inducement culture medium, carries out initial culture, treat to grow at the axillalry bud of outer implant
Multiple Buds；The formula of bud inducement culture medium is: PVP, 30g/L's of NAA, 5.0g/L of 6-BA, 0.2mg/L of MS, 1.0mg/L
Sucrose, the agar of 5.4g/L, pH 5.5；The temperature of initial culture is 26 DEG C, and light application time is 16h every day, light intensity 2000Lx；
3) successive transfer culture: isolate the single twig of 2cm in Multiple Buds, proceeds to cultivate in proliferated culture medium, treats twig
Base portion grow the Multiple Buds of 3.5cm；The formula of proliferated culture medium is: IBA, 30g/ of 6-BA, 0.2mg/L of MS, 1.0mg/L
The sucrose of L, the agar of 5.4g/L, pH 5.5；The temperature of successive transfer culture is 26 DEG C, and light application time is 18h every day, light intensity
2200Lx；
4) root culture: Multiple Buds is separated into single twig again, twig is accessed in root media and takes root
Cultivate 52 days, form Seedling of taking root；The formula of root media is: the sucrose of IBA, 30g/L of 1/2MS, 0.2mg/L, 5.4g/L
Agar, pH 5.5；The temperature of root culture is 26 DEG C, and light application time is 14h every day, light intensity 2400Lx.
5) transplant: by Seedling of taking root in root media at natural light, ambient temperatare is put 1 day, then transplants, transplanting medium
For ratio of weight and number, the Vermiculitum peat composed of rotten mosses: leaf mould is 1:2:2.Transplanting processes the MS nutrition spraying dilution 3 times the same day on blade face
Liquid, then sprays the MS nutritional solution of dilution 3 times, sprays 5 times altogether on every circumference blade face.
Embodiment 3
1, the method for tissue culture of special beautiful Caulis et folium euphorbiae milii, including:
1) outer implant is chosen: on the beautiful Caulis et folium euphorbiae milii of spy in May, cut the current-year branch containing axillalry bud, enter over cleaning, disappear
Current-year branch is cut the stem section containing an axillalry bud, as outer implant after poison in aseptic operating platform；The method of sterilization is: will
Current-year branch after cleaning up is immersed in the ethanol postincubation 30 seconds of 75%, then with aseptic water washing current-year branch 4 times,
Rinse 2 minutes every time, then be immersed in the mercuric chloride of 0.1% process 10 minutes, use aseptic water washing 4 times the most again, rush every time
Wash 2 minutes.
2) initial culture: outer implant is accessed bud inducement culture medium, carries out initial culture, treat to grow at the axillalry bud of outer implant
Multiple Buds；The formula of bud inducement culture medium is: PVP, 30g/L's of NAA, 5.0g/L of 6-BA, 0.2mg/L of MS, 1.0mg/L
Sucrose, the agar of 5.4g/L, pH 5.3；The temperature of initial culture is 25 DEG C, and light application time is 15h every day, light intensity 1900Lx；
3) successive transfer culture: isolate the single twig of 1.8cm in Multiple Buds, proceeds to cultivate in proliferated culture medium, treats tender
The base portion of branch grows the Multiple Buds of 3.3cm；The formula of proliferated culture medium is: the IBA of 6-BA, 0.2mg/L of MS, 1.0mg/L,
The sucrose of 30g/L, the agar of 5.4g/L, pH 5.3；The temperature of successive transfer culture is 25 DEG C, and light application time is 17h every day, light intensity
2100Lx；
4) root culture: Multiple Buds is separated into single twig again, twig is accessed in root media and takes root
Cultivate 50 days, form Seedling of taking root；The formula of root media is: the sucrose of IBA, 30g/L of 1/2MS, 0.2mg/L, 5.4g/L
Agar, pH 5.3；The temperature of root culture is 25 DEG C, and light application time is 13h every day, light intensity 2300Lx.
5) transplant: by Seedling of taking root in root media at natural light, ambient temperatare is put 1 day, then transplants, transplanting medium
For ratio of weight and number, the Vermiculitum peat composed of rotten mosses: leaf mould is 1:1.5:1.5.Transplanting processes the MS spraying dilution 10 times the same day on blade face
Nutritional solution, then sprays the MS nutritional solution of dilution 10 times, sprays 6 times altogether on every circumference blade face.
Above example cultivates the rooting rate of the beautiful Caulis et folium euphorbiae milii of spy, the survival rate obtained, and breeding coefficient is shown in Table 1
Breeding coefficient is whole reproductive bud number/inoculation bud number.
Rooting rate be taken root tissue cultured seedling number × 100% of tissue cultured seedling number/whole.
Survival rate is quantity/total quantity × 100% survived
Table 1
| Special beautiful Caulis et folium euphorbiae milii | Breeding coefficient | Rooting rate | Survival rate |
| Embodiment 1 | 4.26 | 91% | 90% |
| Embodiment 2 | 4.51 | 95% | 93% |
| Embodiment 3 | 4.34 | 93% | 91% |
Claims (5)
1. a method for tissue culture for the beautiful Caulis et folium euphorbiae milii of spy, including:
1) outer implant is chosen: on special beautiful Caulis et folium euphorbiae milii, cut the current-year branch containing axillalry bud, aseptic behaviour after over cleaning, sterilization
Current-year branch is cut by station the stem section containing an axillalry bud, as outer implant；
2) initial culture: outer implant is accessed bud inducement culture medium, carries out initial culture, treat to grow at the axillalry bud of outer implant to grow thickly
Bud；
3) successive transfer culture: isolate 1.6～2cm single twigs in Multiple Buds, proceed to successive transfer culture in proliferated culture medium, treat
The base portion of twig grows the Multiple Buds of 3.1～3.5cm；
4) root culture: Multiple Buds is separated into single twig again, twig is accessed and carries out root culture in root media
48～52 days, form Seedling of taking root；
5) transplant: by taking root in root media, Seedling is placed 1～2 day in natural light, then transplants, and transplanting medium is weight
Portion rate, the Vermiculitum peat composed of rotten mosses: leaf mould is 1:1～2:1～2；
The formula of described bud inducement culture medium is: PVP, 30g/L of NAA, 5.0g/L of 6-BA, 0.2mg/L of MS, 1.0mg/L
Sucrose, the agar of 5.4g/L, pH 5.2～5.5；
The formula of described proliferated culture medium is: the sucrose of IBA, 30g/L of 6-BA, 0.2mg/L of MS, 1.0mg/L, 5.4g/L
Agar, pH 5.2～5.5；
The formula of described root media is: the sucrose of IBA, 30g/L of 1/2MS, 0.2mg/L, the agar of 5.4g/L, pH 5.2
～5.5.
The method for tissue culture of the beautiful Caulis et folium euphorbiae milii of spy the most according to claim 1, it is characterised in that: described step 1) middle sterilization
Method is: the current-year branch after cleaning up be immersed in 75% ethanol postincubation 20～40 seconds, then use aseptic water washing
Current-year branch 3～5 times, rinse 1～3 minute every time, then is immersed in the mercuric chloride of 0.1% process 8～12 minutes, the most again
With aseptic water washing 3～5 times, rinse 1～3 minute every time.
The method for tissue culture of the beautiful Caulis et folium euphorbiae milii of spy the most according to claim 1, it is characterised in that: the temperature of described initial culture
Being 24～26 DEG C, light application time is every day 14～16h, intensity of illumination 1800～2000Lx；The temperature of described successive transfer culture is 24
～26 DEG C, light application time is every day 16～18h, intensity of illumination 2000～2200Lx；The temperature of described root culture is 24～26
DEG C, light application time is every day 12～14h, intensity of illumination 2200～2400Lx.
The method for tissue culture of the beautiful Caulis et folium euphorbiae milii of spy the most according to claim 1, it is characterised in that: described transplanting process the same day to
Spray the MS nutritional solution of dilution 1～10 times on blade face, then spray the MS nutritional solution of dilution 1～10 times on every circumference blade face, altogether
Spray 4～6 times.
The method for tissue culture of the beautiful Caulis et folium euphorbiae milii of spy the most according to claim 1, it is characterised in that: described step 1) in Te Lihai
Chinese bush cherry is 4～the beautiful Caulis et folium euphorbiae milii of spy in May.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613980A (en) * | 2016-12-23 | 2017-05-10 | 滁州绿泉生态农业有限公司 | Tissue culture method of North American begonia |
| CN107637520A (en) * | 2017-09-27 | 2018-01-30 | 南京林业大学 | A kind of method for tissue culture of Hubei Chinese flowering crabapple |
| CN108401908A (en) * | 2018-05-07 | 2018-08-17 | 沈阳农业大学 | Culture medium for preventing tissue culture seedlings of gynura bicolor from browning and growing point necrosis and application thereof |
| CN108651282A (en) * | 2018-05-15 | 2018-10-16 | 句容市茂润苗木有限公司 | A kind of breeding method of dwarf apple |
| CN109952957A (en) * | 2019-05-10 | 2019-07-02 | 南京林业大学 | A kind of method for tissue culture and culture medium of Malus spectabilis |
| CN110235786A (en) * | 2019-07-19 | 2019-09-17 | 南京林业大学 | A kind of ' quick breeding by group culture method of butter fruit ' Malus spectabilis |
| CN112400692A (en) * | 2020-11-18 | 2021-02-26 | 南京林业大学 | Tissue culture and rapid propagation method of Chinese flowering crabapple |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101292630A (en) * | 2008-06-18 | 2008-10-29 | 深圳职业技术学院 | Rieger Begonia tissue culture quick replication method |
| CN103155866A (en) * | 2011-12-10 | 2013-06-19 | 天水市果树研究所 | Malus zumi tissue culture rapid propagation seedling raising method |
| CN103636501A (en) * | 2013-12-09 | 2014-03-19 | 武汉市林业果树科学研究所 | Tissue culture and rapid propagation method of Jinjili crabapple |
| CN104686331A (en) * | 2015-02-21 | 2015-06-10 | 杨业云 | Tissue culture and rapid propagation method for malus halliana |
-
2016
- 2016-07-15 CN CN201610559183.XA patent/CN106212274B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101292630A (en) * | 2008-06-18 | 2008-10-29 | 深圳职业技术学院 | Rieger Begonia tissue culture quick replication method |
| CN103155866A (en) * | 2011-12-10 | 2013-06-19 | 天水市果树研究所 | Malus zumi tissue culture rapid propagation seedling raising method |
| CN103636501A (en) * | 2013-12-09 | 2014-03-19 | 武汉市林业果树科学研究所 | Tissue culture and rapid propagation method of Jinjili crabapple |
| CN104686331A (en) * | 2015-02-21 | 2015-06-10 | 杨业云 | Tissue culture and rapid propagation method for malus halliana |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613980A (en) * | 2016-12-23 | 2017-05-10 | 滁州绿泉生态农业有限公司 | Tissue culture method of North American begonia |
| CN107637520A (en) * | 2017-09-27 | 2018-01-30 | 南京林业大学 | A kind of method for tissue culture of Hubei Chinese flowering crabapple |
| CN108401908A (en) * | 2018-05-07 | 2018-08-17 | 沈阳农业大学 | Culture medium for preventing tissue culture seedlings of gynura bicolor from browning and growing point necrosis and application thereof |
| CN108401908B (en) * | 2018-05-07 | 2022-05-13 | 沈阳农业大学 | Culture medium for preventing tissue culture seedlings of gynura bicolor from browning and growing point necrosis and application thereof |
| CN108651282A (en) * | 2018-05-15 | 2018-10-16 | 句容市茂润苗木有限公司 | A kind of breeding method of dwarf apple |
| CN109952957A (en) * | 2019-05-10 | 2019-07-02 | 南京林业大学 | A kind of method for tissue culture and culture medium of Malus spectabilis |
| CN109952957B (en) * | 2019-05-10 | 2020-11-03 | 南京林业大学 | Tissue culture method and culture medium for Chinese flowering crabapple |
| CN110235786A (en) * | 2019-07-19 | 2019-09-17 | 南京林业大学 | A kind of ' quick breeding by group culture method of butter fruit ' Malus spectabilis |
| CN110235786B (en) * | 2019-07-19 | 2022-03-25 | 南京林业大学 | Tissue culture rapid propagation method of malus spectabilis |
| CN112400692A (en) * | 2020-11-18 | 2021-02-26 | 南京林业大学 | Tissue culture and rapid propagation method of Chinese flowering crabapple |
| CN115956504A (en) * | 2023-01-06 | 2023-04-14 | 北京农学院 | Primary culture method for crabapple plants |
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