CN106190819A - A kind of bacterial cellulose product bubble fermentation Apparatus and method for - Google Patents
A kind of bacterial cellulose product bubble fermentation Apparatus and method for Download PDFInfo
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- CN106190819A CN106190819A CN201610643504.4A CN201610643504A CN106190819A CN 106190819 A CN106190819 A CN 106190819A CN 201610643504 A CN201610643504 A CN 201610643504A CN 106190819 A CN106190819 A CN 106190819A
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Abstract
The invention provides a kind of bacterial cellulose product bubble fermentation Apparatus and method for, including: inoculate fermented strains in fermentation liquid and foam；Collect foam, standing for fermentation, to obtain final product；Containing foaming agent and foam stabilizer in described fermentation liquid.Less culture medium is formed uniform and stable foam, makes culture medium everywhere all at gas-liquid surface, make full use of the nutritional labeling of culture medium, both improve the utilization rate of culture medium, and shortened again the time reaching fermentation termination.Step is simple and convenient to operate, practical.
Description
Technical field
The invention belongs to field of microbial fermentation, particularly to a kind of bacterial cellulose product bubble fermentation method equipment and
Method.
Background technology
Bacterial cellulose is exactly receiving of the another kind of Nantural non-toxic synthesized by fermentable in addition to plant cellulose
Rice material, is also micro organism cellulose.The chemical constitution of Bacterial cellulose is as general fibre element, but has general fibre
The advantageous characteristic that element is incomparable.Bacterial cellulose belongs to nano-scale fiber, is the thinnest in current natural fiber, a typical case
Bacterial fibers beam width only have 0.1 μm, and the width of softwood pulp fiber at least 30 μm, even if the width of cotton fiber is also
Being about 15 μm, and Bacterial cellulose is presented in 100% cellulose, very high purity, and has good penetrating
The characteristics such as property, high-tensile and splendid character maintenance ability.
Now, Bacterial cellulose is also extensively used for the surgical dressing business such as artificial skin, gauze, binder and " bandage "
Product.In future, Bacterial cellulose will be applied to the fields such as papermaking, weaving, battery diaphragm, filter material.
Bacterial cellulose fails to realize the technology barrier of industrialization mainly to be had: 1, fermentation level is relatively low, yields poorly, cost
High, price does not support common plant cellulose；2, study and utilize the Cheng Mo of Bacterial cellulose and the Technology of molding further
The most do not solve.When manufacturing the special material containing Bacterial cellulose now, it is first to discongest Bacterial cellulose to be split into as suspension
It is then added to (such as paper pulp, glass fibre etc.) in other raw materials, so inevitably results in the Bacterial cellulose fracture of interpolation,
Affect overall performance, and the addition of Bacterial cellulose is the least (such as to add 0.5%-2% during papermaking can carry in fact
The performance of high paper, affects the function of paper itself on the contrary when addition is big).
Fermentation is produced to the strain of Bacterial cellulose, have the characteristic of uniqueness.They are the most aerobic, total under resting state
Being to grow at gas-liquid surface, the cellulose of thalline secretion simultaneously forms cellulose membrane at gas-liquid surface, strain and culture fluid it
Between formed and intercept, what this mode was unfavorable for culture medium makes full use of the raising with production efficiency.
Summary of the invention
In order to overcome above-mentioned deficiency, the present invention provides a kind of bacterial cellulose product bubble fermentation Apparatus and method for, relatively
Form uniform and stable foam in few culture medium, make culture medium everywhere all at gas-liquid surface, the cellulose that this equipment is fermented into
Skeleton has huge space, and after adding other raw materials, cellulose bundle is continuously, such as: after adding paper pulp, entire paper
In each Bacterial cellulose bundle be substantially and run through from the beginning to the end, ideally save the characteristic of Bacterial cellulose.With
Time, the time of bubble fermentation is short, turnover is fast, and materials application has a extensive future.
Prior art thinks that foam can affect the breathing of bacterium, causes microbiological contamination, taking up space causes raw material to overflow, and is therefore sending out
Avoid the generation of foam during ferment as far as possible.In the present invention, the existence of foam can affect gas exchange really, but in the short time
Interior oxygen does not has depleted, increases gas liquid interfacial area and can accelerate the trans-utilization of culture medium, can quickly form one dilute
The loose cellulose three-dimensional framework dissipated, after constructing the Bacterial cellulose skeleton that space is the biggest, and then guarantee other raw materials of interpolation,
Cellulose bundle is still continuously, it is ensured that product has preferably structural strength.Therefore, the technology that this device is utilized can
The problem in the past discongesting the cellulose bundle fracture that Bacterial cellulose causes with effective solution.On the other hand, the anti-miscellaneous bacteria of strain itself
Pollution capacity is very strong, is equipped with the problem that existing steam processes or cleaning shop is fully able to get rid of miscellaneous bacteria interference.
To achieve these goals, the present invention adopts the following technical scheme that
A kind of device for Bacterial cellulose foaming fermentation, including: hollow cavity；The top of described cavity is provided with gas
Bubble discharger；The bottom of described cavity is provided with air intake installation.
In the present invention, hollow cavity is that the foaming of fermentation liquid provides place and space, during use, will after amplification thalline and
Fermentation liquid, foaming substance are injected in hollow cavity together, are provided the gas of necessity for fermentation liquid foaming by air intake installation simultaneously
Body, is produced a large amount of bubbles, now, then is derived by bubble discharge device by bubble in cavity, preferred scheme is for exporting to send out
On ferment tray, thalline is proceeded static fermentation.
Arranging air intake installation in the bottom of cavity, top arranges bubble discharge device, maximized guarantee gas and fermentation
Liquid is sufficiently mixed, and simultaneously facilitates the timely discharge of bubble.
Preferably, described bubble discharge device is bubble output hole, and the aperture of described bubble output hole is cavity cross section major diameter
1/11～1/16.Bubble output hole is due to simple in construction, easy to make, it is possible to meets bubble in most cases and discharges requirement.Grind
Study carefully middle discovery, for the bubble fermentation technique of Bacterial cellulose, when 1/ that the aperture of bubble output hole is cavity cross section major diameter
When 11～1/16, bubble expulsion efficiency is the highest, and farthest avoids the external environment impact on chamber internal milieu.
Preferably, the upper end of described air intake installation is provided with backstop.By the peptizaiton of backstop, it is also possible to increase further
The uniformity of strong gas distribution, improves fermentation, bubbling efficiency.
Preferably, it is provided with agitating device in described cavity.Suitable stirring can be effectively improved bubbling efficiency, makes sky
Gas is more uniformly distributed with the mixing of fermentation liquid, meanwhile, can also be efficiently controlled the generation speed of foam by the control of mixing speed
Rate, avoids foam bulk deposition occur substantially.
A kind of system for Bacterial cellulose foaming fermentation, including above-mentioned fine for antibacterial of: fermentation tray, any one
Device, aerator and the control system of dimension element foaming fermentation；Described fermentation tray is connected with bubble discharge device, described aerator
Being connected with air intake installation, described control system is above-mentioned with described fermentation tray, any one respectively to foam for Bacterial cellulose
The device of fermentation, described aerator are connected.
Gas is delivered into hollow cavity by air intake installation by aerator, and fermentation liquid foams in hollow cavity, and by gas
Bubble is transported to ferment on tray by bubble discharger, carries out static fermentation, during whole service, by control system control
Make the operational factor of each device.
The level height of described aerator is not less than the liquid level in described device.Aerator height is less than liquid level
Time, when shutting down or start-up phase may cause the refluence of liquid, block pipeline, cause unnecessary biological pollution.
Preferably, described bubble discharge device is bubble output hole, and the aperture of described bubble output hole is cavity cross section major diameter
1/11～1/16.Bubble output hole is due to simple in construction, easy to make, it is possible to meets bubble in most cases and discharges requirement.Grind
Study carefully middle discovery, for the bubble fermentation technique of Bacterial cellulose, when 1/ that the aperture of bubble output hole is cavity cross section major diameter
When 11～1/16, bubble expulsion efficiency is the highest, and farthest avoids the external environment impact on chamber internal milieu.
Preferably, the upper end of described air intake installation is provided with backstop.By the peptizaiton of backstop, it is also possible to increase further
The uniformity of strong gas distribution, improves fermentation, bubbling efficiency.
Preferably, it is provided with agitating device in described cavity.Suitable stirring can be effectively improved bubbling efficiency, makes sky
Gas is more uniformly distributed with the mixing of fermentation liquid, meanwhile, can also be efficiently controlled the generation speed of foam by the control of mixing speed
Rate, avoids foam bulk deposition occur substantially.
Present invention also offers a kind of bacterial cellulose product, described bacterial cellulose product is three-dimensional network skeleton knot
Structure, in described framing structure, internetwork gap length is more than 1mm.
In the present invention, described three-dimensional network framing structure can be a kind of three dimensional matrix material being similar to sponge.
The space of the bacteria cellulose film of liquid standing for fermentation is at several microns, the sky of the cellulose skeleton of bubble fermentation
Gap, up to several millimeters, facilitates follow-up other functional materials that uniformly add in cellulose space and then flattens film forming, as
Add other plant fiber to make toughness and the fabulous specialties of stretching resistance, add silver simple substance to make antibacterial cellulose
Film, even interpolation medicine make the preparation of slow-releasing.
In the present invention, " bubble fermentation " refers to: " produces substantial amounts of, stable bubble in bacterial fermentation incubation, makes
Culture medium everywhere is all at gas-liquid surface.”
A kind of Bacterial cellulose bubble fermentation method, including:
Inoculate fermented strains into fermentation liquid, make fermentation liquid foam；Collect foam, standing for fermentation, to obtain final product；
Containing foaming agent A and foam stabilizer B in described fermentation liquid.
Foaming agent and foam stabilizer in the present invention are preferably following kind, but are not limited to following kind, general and
Speech, as long as the material of the bubble that ferments can be met in fermentation liquid, the method that all can be used for the present invention.
Preferably, described foaming agent A is alkyl alcohol ethoxylates AEO, alkylphenol polyoxyethylene APEO, acid amide type:
Lauroyl diethanolamine, polyoxyethylene laural amide, cocos nucifera oil acyl diethanolamine, dodecyl ammonium propanoic acid inner salt, dodecyl
At least one in dimethyl betaine, dodecyl dimethyl sulfobetaines or lauroylamidopropyl betaine (LMB)；
Preferably, described foam stabilizer B is polyvinyl alcohol (PVA), hydroxyethyl cellulose (HEC), carboxymethyl cellulose
(CMC), hydroxypropyl methyl cellulose (HPMC), methylcellulose, modified starch, polyacrylamide, polyacrylate, polyoxy second
At least one in alkene fat acyl alcohol amine, synthetic gum tragacanth, gelatin, arabic gum or alkyl dimethyl (OA).
The size of surface tension of liquid directly affects the formation of bubble, and surface tension is the lowest, and its bubble is the most easily formed.
The present invention selects ether type, acid amide type, amino acid pattern and betaine type foaming agent can improve foamability and the coefficient of foaming of solution,
But the foam stabilization time of such foaming agent is short, such foam can be vanished before bacterial secretory cellulose.To this end, the present invention uses
Tackifying stabilizer or high score subclass foam stabilizer, improve the viscoelasticity of liquid film, while the stabilization time improving foam, make foaming
Multiple is not affected substantially.
When the mass fraction of foaming agent is more than 5%, thalline can be by damage in various degree；When the quality of foaming agent is divided
When number is less than 1%, very little, bacterial fibers skeleton gap length is little, poor toughness for the generation amount of bubble.
When the mass fraction of foam stabilizer is more than 10%, the viscoelasticity of liquid film is excessive, is unfavorable for that cellulose is at foam surface
Growth；When the mass fraction of foam stabilizer is less than 2%, bubble exists that the time is shorter, foam is easily before bacterial secretory cellulose
Vanish.
Therefore, in the fermentation liquid of the present invention, the mass fraction of preferred foaming agent is 1～5%, the mass fraction of foam stabilizer
It is 2～10%.
When ventilation is more than 1:1.2m3/m3Min, dissolved oxygen content promotes inconspicuous, the less stable of foam.Work as ventilation
Amount is less than 1:0.6m3/m3During min, dissolved oxygen content is relatively low, and the yield of foam is relatively low.Therefore, in order to ensure that enough gas-liquids connect
The time of touching and dissolved oxygen exist, and in the present invention, preferred ventilation is 1:0.6～1.2m3/m3min。
Preferably, the condition of described static fermentation is 25～30 DEG C of bottom fermentation 10-12h；
Preferably, the preparation method of described fermented bacterium includes: the activation of strain, inoculation, phage amplification；
Present invention also offers one preferably Bacterial cellulose bubble fermentation method, including:
1) preparation of strain.Take-80 DEG C preserve strains, shift-20 DEG C place 12h, shift 4 DEG C place about 2-4h until
All dissolve.
2) take 1ml bacterium solution to be inoculated in 150ml seed culture fluid, cultivate 24h, become primary seed solution for 28 DEG C.
3) take primary seed solution to be inoculated in 500ml fermentation liquid, it is therefore an objective to carry out phage amplification, fermentation liquid and seed culture
The formula of liquid is different.Cultivate 18～24h for 30 DEG C.
4) fermentation liquid adds 1% foaming agent A and 2% foam stabilizer B, is sufficiently mixed in the airtight bottle of 500ml.
5) fermentation liquid is imported foaming cylinder, foaming cylinder be one can the PE bucket of high temperature sterilize, bottom has air inlet, top to go out
Stenostomy, pumps into air by lasting, makes to bubble above fermentation liquid, transfers to foam, in fermentation dish, carry out standing for fermentation,
28 DEG C, 10-12h.
Fermentation liquid in the present invention, the compound method of seed culture fluid use the conventional formulation method of this area.Such as:
Multiple formulations described in Chinese patent CN201110063655.X.
Present invention also offers a kind of fermentation liquid for Bacterial cellulose foaming fermentation, former by following percentage by weight
Material composition: foaming agent 1～5%, foam stabilizer 2～10%；Remaining is fermentation liquid.
Bacterial cellulose product provided in the present invention, or the bacterial cellulose product prepared of described method/equipment
Property indices all can reach the requirement of relevant international and national standard；In actual applications (such as: copy paper, antibacterial
Cellulose membrane or the manufacture etc. of slow-releasing preparation), it is thus achieved that the property that the bacteria cellulose film of more existing static fermentation is more excellent
Energy.
Beneficial effects of the present invention
(1) present invention provides a kind of bubble fermentation method, less culture medium is formed uniform and stable foam, makes everywhere
Culture medium, all at gas-liquid surface, makes full use of the nutritional labeling of culture medium, had both improve the utilization rate of culture medium, and had shortened again and reach
Time to fermentation termination.
(2) prior art thinks that foam can affect the breathing of bacterium, causes microbiological contamination, taking up space causes raw material to overflow, therefore
Avoid the generation of foam the most as far as possible.In the present invention, foam exists can affect gas exchange really, but in short-term
Interior oxygen does not has depleted, increases gas liquid interfacial area and can accelerate the trans-utilization of culture medium, can quickly form one
Sparse loose cellulose three-dimensional framework, constructs the Bacterial cellulose skeleton that space is the biggest, and then ensures to add other raw materials
After, cellulose bundle is still continuously, it is ensured that product has preferably structural strength.Therefore, the skill that this device is utilized
The problem that art can effectively solve to discongest the cellulose bundle fracture that Bacterial cellulose causes in the past.On the other hand, strain itself resists
Living contaminants is very capable, is equipped with the problem that existing steam processes or cleaning shop is fully able to get rid of miscellaneous bacteria interference.
(3) preparation method of the present invention is simple, fermentation time is short, practical.
(4) apparatus of the present invention simple in construction, easy to operate, easy to spread.
Accompanying drawing explanation
Fig. 1 is assembly of the invention structure chart.
Fig. 2 be the cellulose products that bubble fermentation method of the present invention is made SEM figure, wherein, it is seen that skeleton be not single
Cellulose bundle, but antibacterial is in foam and foam junction, the place that culture medium is relatively more, and a large amount of cellulose bundles of secretion coagulate
It is polymerized to.And at some places, the cellulose bundle of bacterial secretory also can form the film of a bubble shape.
Fig. 3 is the SEM figure of the cellulose products using common standing for fermentation.
Wherein, 1. fermentation tray, 2. cylinder, 3. aerator, 4. steam hose, 5. handle, 6. agitator, the most unidirectional give vent to anger
Mouth, 8. bubble output hole, 9 sealing lids.
Fig. 4 is that foam stabilizer affects figure to cellulose output.
Fig. 5 is that foam stabilizer foam stability can be schemed.
Fig. 6 is that foam static fermentation is to yield effect figure.
Detailed description of the invention
By the following examples feature of present invention and other correlated characteristic are described in further detail, in order to of the same trade
The understanding of technical staff:
Embodiment 1
A kind of device for Bacterial cellulose foaming fermentation, including: hollow cavity；The top of described cavity is provided with gas
Bubble discharger；The bottom of described cavity is provided with air intake installation.
In the present invention, hollow cavity is that the foaming of fermentation liquid provides place and space, during use, will after amplification thalline and
Fermentation liquid, foaming substance are injected in hollow cavity together, provide necessity by air intake installation for bacterial fermentation foaming simultaneously
Gas, is produced a large amount of bubbles, now, then is derived by bubble discharge device by bubble in cavity, preferred scheme is for exporting to
On fermentation tray, thalline is proceeded static foaming.
Arranging air intake installation in the bottom of cavity, top arranges bubble discharge device, maximized guarantee gas and fermentation
Liquid is sufficiently mixed, and simultaneously facilitates the timely discharge of bubble.
Specifically, said apparatus can as shown in Figure 1, and wherein, described hollow cavity is cylinder 2, and cylinder 2 top is arranged
Having sealing lid 9, be used for feeding and sealing, the side at cylinder 2 top is provided with bubble output hole 8, is used for bubble fermentation process
The bubble of middle generation is discharged in height fermentation tray 1, and the side of cylinder 2 is provided with handle 5, it is simple to the transport of cylinder 2 and installation, cylinder
The lower end of body 2 is provided with unidirectional gas outlet 7 for required air is imported cylinder 2, and the side of unidirectional gas outlet 7 is additionally provided with
Agitator 6, is used for stirring fermentation liquid and makes it mix homogeneously with gas and foaming substance as early as possible, and the air inlet section of unidirectional gas outlet 7 is led to
Cross steam hose 4 to be connected with aerator 3, it is provided that the source of gas.
Embodiment 2
A kind of device for Bacterial cellulose foaming fermentation, including: hollow cavity；The top of described cavity is provided with gas
Bubble discharger；The bottom of described cavity is provided with air intake installation.
Described bubble discharge device is bubble output hole, and the aperture of described bubble output hole is the 1/11～1/ of cavity cross section major diameter
16.Bubble output hole is due to simple in construction, easy to make, it is possible to meets bubble in most cases and discharges requirement.Research finds,
For the bubble fermentation technique of Bacterial cellulose, when 1/11～1/16 that the aperture of bubble output hole is cavity cross section major diameter
Time, bubble expulsion efficiency is the highest, and farthest avoids the external environment impact on chamber internal milieu.
Embodiment 3
A kind of device for Bacterial cellulose foaming fermentation, including: hollow cavity；The top of described cavity is provided with gas
Bubble discharger；The bottom of described cavity is provided with air intake installation.
The upper end of described air intake installation is provided with backstop.By the peptizaiton of backstop, it is also possible to heighten gas further
Uniformity, improves fermentation, bubbling efficiency.
Embodiment 4
A kind of device for Bacterial cellulose foaming fermentation, including: hollow cavity；The top of described cavity is provided with gas
Bubble discharger；The bottom of described cavity is provided with air intake installation.
It is provided with agitating device in described cavity.Suitable stirring can be effectively improved bubbling efficiency, makes air and sends out
The mixing of ferment liquid is more uniformly distributed, and meanwhile, can also be efficiently controlled the generating rate of foam by the control of mixing speed, maximum
Avoid the generation of the bulk deposition phenomenon of foam with changing.
Embodiment 5
A kind of system for Bacterial cellulose foaming fermentation, including: fermentation tray, fermentation of foaming for Bacterial cellulose
Device, aerator and control system；Described fermentation tray is connected with bubble discharge device, described aerator and air intake installation phase
Even, described control system is respectively with described fermentation tray, foam the device of fermentation, described aerator phase for Bacterial cellulose
Even.
Wherein, the described device for Bacterial cellulose foaming fermentation, including: hollow cavity；The top of described cavity sets
It is equipped with bubble discharge device；The bottom of described cavity is provided with air intake installation.
Specifically, said apparatus can as shown in Figure 1, and wherein, described hollow cavity is cylinder 2, and cylinder 2 top is arranged
Having sealing lid 9, be used for feeding and sealing, the side at cylinder 2 top is provided with bubble output hole 8, is used for bubble fermentation process
The bubble of middle generation is discharged in height fermentation tray 1, and the side of cylinder 2 is provided with handle 5, it is simple to the transport of cylinder 2 and installation, cylinder
The lower end of body 2 is provided with unidirectional gas outlet 7 for required air is imported cylinder 2, and the side of unidirectional gas outlet 7 is additionally provided with
Agitator 6, is used for stirring fermentation liquid and makes it mix homogeneously with gas and foaming substance as early as possible, and the air inlet section of unidirectional gas outlet 7 is led to
Cross steam hose 4 to be connected with aerator 3, it is provided that the source of gas.
Gas is delivered into hollow cavity by air intake installation by aerator, and fermentation liquid completes foaming in hollow cavity, and
It is transported to ferment on tray by bubble by bubble discharge device, carries out static fermentation, during whole service, pass through control system
Control the operational factor of each device.
Embodiment 6
A kind of system for Bacterial cellulose foaming fermentation, including: fermentation tray, fermentation of foaming for Bacterial cellulose
Device, aerator and control system；Described fermentation tray is connected with bubble discharge device, described aerator and air intake installation phase
Even, described control system is respectively with described fermentation tray, foam the device of fermentation, described aerator phase for Bacterial cellulose
Even.
Wherein, the described device for Bacterial cellulose foaming fermentation, including: hollow cavity；The top of described cavity sets
It is equipped with bubble discharge device；The bottom of described cavity is provided with air intake installation.
The level height of described aerator is not less than the liquid level in described device.Aerator height is less than liquid level
Time, when shutting down or start-up phase may cause the refluence of liquid, block pipeline, cause unnecessary biological pollution.
Embodiment 7
A kind of system for Bacterial cellulose foaming fermentation, including: fermentation tray, fermentation of foaming for Bacterial cellulose
Device, aerator and control system；Described fermentation tray is connected with bubble discharge device, described aerator and air intake installation phase
Even, described control system is respectively with described fermentation tray, foam the device of fermentation, described aerator phase for Bacterial cellulose
Even.
Wherein, the described device for Bacterial cellulose foaming fermentation, including: hollow cavity；The top of described cavity sets
It is equipped with bubble discharge device；The bottom of described cavity is provided with air intake installation.
Described bubble discharge device is bubble output hole, and the aperture of described bubble output hole is the 1/11～1/ of cavity cross section major diameter
16.Bubble output hole is due to simple in construction, easy to make, it is possible to meets bubble in most cases and discharges requirement.Research finds,
For the bubble fermentation technique of Bacterial cellulose, when 1/11～1/16 that the aperture of bubble output hole is cavity cross section major diameter
Time, bubble expulsion efficiency is the highest, and farthest avoids the external environment impact on chamber internal milieu.
Embodiment 8
A kind of system for Bacterial cellulose foaming fermentation, including: fermentation tray, fermentation of foaming for Bacterial cellulose
Device, aerator and control system；Described fermentation tray is connected with bubble discharge device, described aerator and air intake installation phase
Even, described control system is respectively with described fermentation tray, foam the device of fermentation, described aerator phase for Bacterial cellulose
Even.
Wherein, the described device for Bacterial cellulose foaming fermentation, including: hollow cavity；The top of described cavity sets
It is equipped with bubble discharge device；The bottom of described cavity is provided with air intake installation.
The upper end of described air intake installation is provided with backstop.By the peptizaiton of backstop, it is also possible to heighten gas further
Uniformity, improves fermentation, bubbling efficiency.
Embodiment 9
A kind of system for Bacterial cellulose foaming fermentation, including: fermentation tray, fermentation of foaming for Bacterial cellulose
Device, aerator and control system；Described fermentation tray is connected with bubble discharge device, described aerator and air intake installation phase
Even, described control system is respectively with described fermentation tray, foam the device of fermentation, described aerator phase for Bacterial cellulose
Even.
Wherein, the described device for Bacterial cellulose foaming fermentation, including: hollow cavity；The top of described cavity sets
It is equipped with bubble discharge device；The bottom of described cavity is provided with air intake installation.
It is provided with agitating device in described cavity.Suitable stirring can be effectively improved bubbling efficiency, makes air and sends out
The mixing of ferment liquid is more uniformly distributed, and meanwhile, can also be efficiently controlled the generating rate of foam by the control of mixing speed, maximum
Avoid the generation of the bulk deposition phenomenon of foam with changing.
Embodiment 10
One preferably Bacterial cellulose bubble fermentation method, including:
1) preparation of strain.Take-80 DEG C preserve strains, shift-20 DEG C place 12h, shift 4 DEG C place about 2-4h until
All dissolve.
2) take 1ml bacterium solution to be inoculated in 150ml seed culture fluid, cultivate 24h, become primary seed solution for 28 DEG C.
3) take primary seed solution to be inoculated in 500ml fermentation liquid, it is therefore an objective to carry out phage amplification, fermentation liquid and seed culture
The formula of liquid is different.Cultivate 18～24h for 30 DEG C.
4) fermentation liquid adds 1% foaming agent A and 2% foam stabilizer B, is sufficiently mixed in the airtight blue lid bottle of 500ml.
5) fermentation liquid is imported foaming cylinder, foaming cylinder be one can the PE bucket of high temperature sterilize, bottom has air inlet, top to go out
Stenostomy, pumps into air by lasting, makes to bubble above fermentation liquid, transfers to foam, in fermentation dish, carry out standing for fermentation,
28 DEG C, 10h.
Result and detection:
As shown in Figure 4: foaming agent A and foam stabilizer B is little on the impact of yield.
As it is shown in figure 5, consider production cost, foam stability and three factors of cellulose output, take 1.3%
Infusion A and the combination of 1.8% foam stabilizer B, effect is best.
As shown in Figure 6, in terms of test structure, bubble fermentation substantially reduces the production cycle, improves yield.
It addition, bubble fermentation is big because of its specific surface area, takes full advantage of the nutritional labeling of culture fluid, reduce and produce into
This.
Embodiment 11
One preferably Bacterial cellulose bubble fermentation method, including:
1) preparation of strain.Take-80 DEG C preserve strains, shift-20 DEG C place 12h, shift 4 DEG C place about 2-4h until
All dissolve.
2) take 1ml bacterium solution to be inoculated in 150ml seed culture fluid, cultivate 24h, become primary seed solution for 28 DEG C.
3) take primary seed solution to be inoculated in 500ml fermentation liquid, it is therefore an objective to carry out phage amplification, fermentation liquid and seed culture
The formula of liquid is different.Cultivate 18～24h for 30 DEG C.
4) fermentation liquid adds 5% foaming agent A and 10% foam stabilizer B, is sufficiently mixed in the airtight bottle of 500ml.
5) fermentation liquid is imported foaming cylinder, foaming cylinder be one can the PE bucket of high temperature sterilize, bottom has air inlet, top to go out
Stenostomy, pumps into air by lasting, makes to bubble above fermentation liquid, transfers to foam, in fermentation dish, carry out standing for fermentation,
28 DEG C, 12h.
Wherein, foaming agent A is alkylphenol polyoxyethylene APEO；Foam stabilizer B is polyvinyl alcohol (PVA).
Embodiment 12
One preferably Bacterial cellulose bubble fermentation method, including:
1) preparation of strain.Take-80 DEG C preserve strains, shift-20 DEG C place 12h, shift 4 DEG C place about 2-4h until
All dissolve.
2) take 1ml bacterium solution to be inoculated in 150ml seed culture fluid, cultivate 24h, become primary seed solution for 28 DEG C.
3) take primary seed solution to be inoculated in 500ml fermentation liquid, it is therefore an objective to carry out phage amplification, fermentation liquid and seed culture
The formula of liquid is different.Cultivate 18～24h for 30 DEG C.
4) fermentation liquid adds 3% foaming agent A and 6% foam stabilizer B, is sufficiently mixed in the airtight bottle of 500ml.
5) fermentation liquid is imported foaming cylinder, foaming cylinder be one can the PE bucket of high temperature sterilize, bottom has air inlet, top to go out
Stenostomy, pumps into air by lasting, makes to bubble above fermentation liquid, transfers to foam, in fermentation dish, carry out standing for fermentation,
28 DEG C, 11.5h.
Wherein, foaming agent A is alkyl alcohol ethoxylates AEO；Described foam stabilizer B is alkyl dimethyl (OA)
In.
Embodiment 13
One preferably Bacterial cellulose bubble fermentation method, including:
1) preparation of strain.Take-80 DEG C preserve strains, shift-20 DEG C place 12h, shift 4 DEG C place about 2-4h until
All dissolve.
2) take 1ml bacterium solution to be inoculated in 150ml seed culture fluid, cultivate 24h, become primary seed solution for 28 DEG C.
3) take primary seed solution to be inoculated in 500ml fermentation liquid, it is therefore an objective to carry out phage amplification, fermentation liquid and seed culture
The formula of liquid is different.Cultivate 18～24h for 30 DEG C.
4) fermentation liquid adds 3% foaming agent A and 6% foam stabilizer B, is sufficiently mixed in the airtight bottle of 500ml.
5) fermentation liquid is imported foaming cylinder, foaming cylinder be one can the PE bucket of high temperature sterilize, bottom has air inlet, top to go out
Stenostomy, pumps into air by lasting, makes to bubble above fermentation liquid, transfers to foam, in fermentation dish, carry out standing for fermentation,
28 DEG C, 11.5h.
Wherein, foaming agent A is lauroyl diethanolamine, polyoxyethylene laural amide and cocos nucifera oil acyl diethanolamine；Three's
Mass ratio is 1:1:1.
Foam stabilizer B is hydroxyethyl cellulose (HEC), gelatin, and the mass ratio of three is 1:1.
Embodiment 14
One preferably Bacterial cellulose bubble fermentation method, including:
1) preparation of strain.Take-80 DEG C preserve strains, shift-20 DEG C place 12h, shift 4 DEG C place about 2-4h until
All dissolve.
2) take 1ml bacterium solution to be inoculated in 150ml seed culture fluid, cultivate 24h, become primary seed solution for 28 DEG C.
3) take primary seed solution to be inoculated in 500ml fermentation liquid, it is therefore an objective to carry out phage amplification, fermentation liquid and seed culture
The formula of liquid is different.Cultivate 18～24h for 30 DEG C.
4) fermentation liquid adds 5% foaming agent A and 10% foam stabilizer B, is sufficiently mixed in the airtight bottle of 500ml.
5) fermentation liquid is imported foaming cylinder, foaming cylinder be one can the PE bucket of high temperature sterilize, bottom has air inlet, top to go out
Stenostomy, by the lasting air that pumps into, (air inflow/ventilation of air is 1:0.6～1.2m3/m3Min), make on fermentation liquid
Bubbling in side, is transferred to by foam in fermentation dish, carries out standing for fermentation, 28 DEG C, 12h.
Wherein, foaming agent A is dodecyl ammonium propanoic acid inner salt and lauroylamidopropyl betaine (LMB), the matter of the two
Amount ratio is 1:3.
Foam stabilizer B is hydroxyethyl cellulose (HEC), polyacrylamide and arabic gum, and the mass ratio of three is 1:1.5:
1.2。
Result shows: use the bacterial cellulose product that described in embodiment of the present invention 1-13 prepared by equipment or method to have
3 D stereo network structure, space is big and uniform, as shown in Figure 2.This is owing to bubble limits the travelling path of antibacterial, antibacterial
Liquid film only along bubble moves about, so that the cellulose bundle of secretion forms this stereochemical structure, is similar to " sponge "
Three dimensional structure.
And the bacterial cellulose product space that uses existing static fermentation method to produce is little and disorderly and unsystematic, in planar junction
Structure, as shown in Figure 3.This is that secreted cellulose bundle is handed at gas-liquid surface owing to antibacterial moves about at the gas-liquid surface of culture medium
Mistake, is finally made into one " cloth ".
Finally should be noted that and the foregoing is only the preferred embodiments of the present invention, be not limited to this
Bright, although being described in detail the present invention with reference to previous embodiment, for a person skilled in the art, it is still
Technical scheme described in previous embodiment can be modified, or wherein part is carried out equivalent.All at this
Within bright spirit and principle, any modification, equivalent substitution and improvement etc. made, should be included in protection scope of the present invention
Within.Although the detailed description of the invention of the present invention is described by the above-mentioned accompanying drawing that combines, but not to scope
Restriction, one of ordinary skill in the art should be understood that, on the basis of technical scheme, those skilled in the art are not required to
Various amendments that creative work to be paid can be made or deformation are still within protection scope of the present invention.
Claims (10)
1. the device for Bacterial cellulose foaming fermentation, it is characterised in that including: hollow cavity；The top of described cavity
Portion is provided with bubble discharge device；The bottom of described cavity is provided with air intake installation.
2. device as claimed in claim 1, it is characterised in that described bubble discharge device is bubble output hole, described bubble output hole
Aperture is the 1/11～1/16 of the cavity cross section minimum length of side.
3. device as claimed in claim 1, it is characterised in that the upper end of described air intake installation is provided with backstop.
4. device as claimed in claim 1, it is characterised in that be provided with agitating device in described cavity.
5. the system for Bacterial cellulose foaming fermentation, it is characterised in that including: fermentation tray, claim 1-4 are appointed
One described device, aerator and control system；Described fermentation tray is connected with bubble output hole, described aerator and air inlet phase
Even, described control system respectively with the device described in described fermentation tray, described any one of claim 1-4, described aerator
It is connected.
6. system as claimed in claim 5, it is characterised in that the level height of described aerator is not less than in described device
Liquid level.
7. a Bacterial cellulose bubble fermentation method, it is characterised in that including:
Inoculate fermented strains in fermentation liquid, make fermentation liquid foam；Collect foam, standing for fermentation, to obtain final product.
8. method as claimed in claim 7, it is characterised in that containing foaming agent and foam stabilizer in described fermentation liquid.
9. method as above-mentioned in claim 8, it is characterised in that described foaming agent is alkyl alcohol ethoxylates AEO, alkyl phenol
Polyoxyethylene ether APEO, acid amide type: lauroyl diethanolamine, polyoxyethylene laural amide, cocos nucifera oil acyl diethanolamine, dodecyl
Ammonium propanoic acid inner salt, dodecyldimethylammonium hydroxide inner salt, dodecyl dimethyl sulfobetaines or dodecanamide propyl Radix Betae
At least one in alkali (LMB)；
Described foam stabilizer is polyvinyl alcohol (PVA), hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC), hydroxypropyl methyl
Cellulose (HPMC), methylcellulose, modified starch, polyacrylamide, polyacrylate, Polyoxyethylene fatty acyl hydramine, conjunction
At least one in Cheng Long's glue, gelatin, arabic gum or alkyl dimethyl (OA)；
Or in described fermentation liquid, the mass fraction of foaming agent is 1～5%, and the mass fraction of foam stabilizer is 2～10%；
Or during described foaming processes, ventilation is 1:0.6～1.2m3/m3min；
The condition of described static fermentation is 25～30 DEG C of bottom fermentation 10-12h；
The preparation method of described fermented bacterium includes: the activation of strain, inoculation, phage amplification.
10. the bacterial cellulose product that prepared by method described in any one of claim 6-9, it is characterised in that described bacterial fibers
Element product is three-dimensional network framing structure, and in described framing structure, internetwork gap length is more than 1mm.
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| EP3459566A1 (en) * | 2017-09-25 | 2019-03-27 | ETH Zurich | Method for obtaining a self-supporting bacterial foam and the foam obtained by said method |
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| EP3459566A1 (en) * | 2017-09-25 | 2019-03-27 | ETH Zurich | Method for obtaining a self-supporting bacterial foam and the foam obtained by said method |
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Denomination of invention: A foam fermentation equipment and method for bacterial cellulose products Effective date of registration: 20220628 Granted publication date: 20190329 Pledgee: Jinan Free Trade Zone sub branch of Qilu Bank Co.,Ltd. Pledgor: SHANDONG NAMEIDE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022370000080 |