CN106148217A - A kind of mixed fermenting agent for biology cellulose fermentation - Google Patents
A kind of mixed fermenting agent for biology cellulose fermentation Download PDFInfo
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- CN106148217A CN106148217A CN201510138861.0A CN201510138861A CN106148217A CN 106148217 A CN106148217 A CN 106148217A CN 201510138861 A CN201510138861 A CN 201510138861A CN 106148217 A CN106148217 A CN 106148217A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 60
- 230000004151 fermentation Effects 0.000 title claims abstract description 60
- 229920002678 cellulose Polymers 0.000 title claims abstract description 26
- 239000001913 cellulose Substances 0.000 title claims abstract description 26
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 15
- 230000001580 bacterial effect Effects 0.000 claims abstract description 25
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 claims abstract description 19
- 229940050410 gluconate Drugs 0.000 claims abstract description 19
- 241000588902 Zymomonas mobilis Species 0.000 claims abstract description 18
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 17
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 17
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 17
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 17
- 230000004913 activation Effects 0.000 claims abstract description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 7
- 230000003068 static effect Effects 0.000 claims description 5
- PTHCMJGKKRQCBF-UHFFFAOYSA-N Cellulose, microcrystalline Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC)C(CO)O1 PTHCMJGKKRQCBF-UHFFFAOYSA-N 0.000 abstract description 7
- 238000006243 chemical reaction Methods 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 2
- 108010025899 gelatin film Proteins 0.000 abstract description 2
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 11
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 239000002609 medium Substances 0.000 description 6
- 239000000835 fiber Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 229930091371 Fructose Natural products 0.000 description 4
- 239000005715 Fructose Substances 0.000 description 4
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 244000235858 Acetobacter xylinum Species 0.000 description 3
- 235000002837 Acetobacter xylinum Nutrition 0.000 description 3
- 239000008104 plant cellulose Substances 0.000 description 3
- 241000589236 Gluconobacter Species 0.000 description 2
- 238000007792 addition Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 244000099147 Ananas comosus Species 0.000 description 1
- 235000007119 Ananas comosus Nutrition 0.000 description 1
- 229920002749 Bacterial cellulose Polymers 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 229920001340 Microbial cellulose Polymers 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000005016 bacterial cellulose Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000020415 coconut juice Nutrition 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of mixed fermenting agent for biology cellulose fermentation, comprise gluconate pyracetobacillus, zymomonas mobilis and Bifidobacterium and/or lactobacillus acidophilus.Ratio between described gluconate pyracetobacillus, zymomonas mobilis and Bifidobacterium and/or lactobacillus acidophilus is 10-20:7-15:3-8.It is preferably 17:11:5.Described bacterial strain uses front through activation culture.Ratio between described bacterial strain is the volume ratio of the seed liquor obtaining that spreads cultivation again after activation.Mutual reciprocity and mutual benefit between the hybrid bacterial strain of the present invention, form the situation of symbiosis.The time that biological cellulose gel film is formed can be extended, improve carbon source conversion rate of reducing sugar, thus be greatly improved the ability that film is produced in biology cellulose fermentation.
Description
Technical field
The present invention relates to technical field of biological fermentation, especially relate to a kind of mixed fermenting agent for biology cellulose fermentation and the method with its fermenting and preparing biological cellulose.
Background technology
Biology cellulose (Biocellulose), the bacteria cellulose that is otherwise known as (Bacterial cellulose, BC), it is by the microorganism of acetic acid Pseudomonas, the cellulose products made such as fermentations such as acetobacter xylinums.Many with discarded object at present, if coconut water, pineapple peel juice etc. are as raw material, therefore it is with low cost.Containing the moisture of up to more than 95% in the product obtaining, be therefore otherwise known as biological cellulose gel.The preparation method of biological cellulose gel is to it known in the art, as disclosed in all have in CN101671708, CN1840677A, CN101319242A.Biology cellulose without association products such as lignin, pectin and hemicelluloses, has high-crystallinity (up to 95%, plant cellulose for 65%) and the high degree of polymerization (DP value 2 000～8 000) compared with plant cellulose；It has hyperfine network structure, and biological fiber cellulose fiber is to be combined into the thick fibre bundle of 40～60 nanometers by the fento of diameter 3～4 nanometer, and is intertwined to form the hyperfine network structure of prosperity；Its elastic modelling quantity be the several times of general string to more than ten times, and tensile strength is high；Biological fiber have very strong moisture holding capacity, has higher biocompatibility, adaptability and good biodegradability simultaneously.The existing product of biological cellulose gel is mainly used in food, in cosmetics, as being used for making jelly, Yoghourt, increases the absorption of dietary fiber；Or for making the carrier of mask product.
Existing biology cellulose fermenting and producing is all to use single culture fermentation, such as acetobacter xylinum, gluconobacter sp etc.；But these bacterial classifications are when fermentation, are generally of fermentation level relatively low, yield poorly, cost is high, finished product price does not support the defects such as common plant cellulose.Yield of biological cellulose is typically maintained in 4-8g/L.Conversion rate of reducing sugar is also only below 20%, and fermentation level is very low.
Content of the invention
Technical problem solved by the invention is to provide a kind of mixed fermenting agent for biology cellulose fermentation, comprises gluconate pyracetobacillus, zymomonas mobilis and Bifidobacterium and/or lactobacillus acidophilus.
Ratio between wherein said gluconate pyracetobacillus, zymomonas mobilis and Bifidobacterium and/or lactobacillus acidophilus is 10-20:7-15:3-8.It is preferably 17:11:5.
Wherein said bacterial strain uses front through activation culture.Ratio between described bacterial strain is the volume ratio of the seed liquor obtaining that spreads cultivation again after activation.
The present invention is to activate above-mentioned bacterial strains before use in advance, then obtains its corresponding seed liquor after spreading cultivation, then according to aforementioned proportion (seed liquor volume ratio) is inoculated in Biological cellulose fermentation culture medium, carries out fermented and cultured under proper condition.
Described fermentation temperature is preferably 25-35 degree, and fermentation time is 3-10 days.
Described fermentation is tray static fermentation or shaking flask or fermentation tank dynamic fermentation.
During described fermentation, pH value controls between 6.2-7.2.
Containing the carbon source decomposed for described bacterial strain in described fermentation culture medium.
The hybrid bacterial strain that the present invention uses, is particularly well suited to biology cellulose tray static fermentation.Generally, when using acetobacter xylinum or the fermentation of gluconobacter sp single culture, tray static fermentation container i.e. can form transparent membranaceous fiber gel at vessel surface at inoculation fermentation after 1 day, the formation meeting of this gel obstruction fermented and cultured primary surface significantly contacts with air, so that the oxygen content in culture medium is greatly reduced.This can be greatly reduced for the producing level of carbon source such as glucose or fructose for fermented bacterium in follow-up sweat, thus reduces final biological cellulose membrane yield.And the present invention uses at least three kinds of bacterial strain mixing fermentation culture, zymomonas mobilis therein belongs to gramnegative bacterium, micro-aerobic growth, can be by ED metabolic pathway by glucose and fructose converting for ethanol.And its highly acidproof, quite tolerant alcohol, quite tolerant glucose, can grow between pH3.5～7.5, can be without impact growth under the conditions of concentration of alcohol 5.5%, can be with normal growth in containing 20% concentration of glucose environment；The nutritional need of zymomonas mobilis growth simultaneously is relatively easy.So when the hybrid bacterial strain Preliminary fermentation of the present invention, gluconate pyracetobacillus and zymomonas mobilis decompose carbon sources such as utilizing glucose, fructose or sucrose simultaneously, but zymomonas mobilis utilizes reduced sugar ability strong, nutritional need is simple, quick increment, carbon source higher for initial content in culture medium such as glucose, fructose etc. can be resolved into ethanol, ethanol and remaining glucose etc. again can be simultaneously for gluconate pyracetobacillus fermentations, now gluconate pyracetobacillus is dominant strain, and text message starts.Can accumulate the acetic acid of certain content in culture medium, pH value reduces.Now Bifidobacterium or lactobacillus acidophilus or both combinations can decompose acetic acid to a certain extent, regulate pH value；So mutual reciprocity and mutual benefit between hybrid bacterial strain, form the situation of symbiosis.The time that biological cellulose gel film is formed can be extended, improve carbon source conversion rate of reducing sugar, thus be greatly improved the ability that film is produced in biology cellulose fermentation.
Simultaneously, owing to the bacterial strain of the present invention containing the probio such as Bifidobacterium or lactobacillus acidophilus, also can be containing a small amount of probio in the biological cellulose membrane preparing, after washing, the biological cellulose gel containing probio can be obtained, more add be applicable to food, health products and use.
Hybrid bacterial strain of the present invention is equally applicable to biology cellulose dynamic fermentation, produces film ability than the dynamic fermentation of conventional single bacterial strain and also increases.
Detailed description of the invention
Below in conjunction with detailed description of the invention and contrast experiment, the present invention is further illustrated, but implementation is understood not to limitation of the present invention in detail below.Those of ordinary skill in the art can make apparently on the basis of the present invention various change and change, it should all within the scope of the present invention.
Embodiment 1:
A kind of mixed fermenting agent for biology cellulose fermentation, comprises gluconate pyracetobacillus, zymomonas mobilis and Bifidobacterium and/or lactobacillus acidophilus.
Bifidobacterium therein and lactobacillus acidophilus can be containing only one therein, it is also possible to both of which contains.
Microbial inoculum preparation process:
1st, actication of culture
Gluconate pyracetobacillus, zymomonas mobilis, Bifidobacterium, lactobacillus acidophilus cultivate activation respectively on activation medium；
2nd, seed liquor is cultivated
Gluconate pyracetobacillus, zymomonas mobilis, Bifidobacterium, lactobacillus acidophilus inoculate in seed culture medium respectively and spread cultivation, and cultivate to its exponential phase；
3rd, inoculation fermentation
The seed liquor of gluconate pyracetobacillus, zymomonas mobilis and Bifidobacterium and lactobacillus acidophilus according to volume ratio 17:11:5(wherein Bifidobacterium addition be 2 lactobacillus acidophilus additions be 3) ratio inoculate successively in static shallow tray fermentation culture medium, carry out fermented and cultured.
Activation medium in above-mentioned steps, seed culture medium and fermentation medium can use the conventional medium of existing strain culturing, it would however also be possible to employ the culture medium of formulated, to obtain the biological cellulose gel of higher yield.
Embodiment 2:
Other are all same as in Example 1, and mixed fermenting agent is the gluconate pyracetobacillus of 15:8:6, zymomonas mobilis, Bifidobacterium.
Embodiment 3:
Other are all same as in Example 1, and mixed fermenting agent is the gluconate pyracetobacillus of 20:15:8, zymomonas mobilis, lactobacillus acidophilus.
Embodiment 4:
Other are all same as in Example 1, and mixed fermenting agent is the gluconate pyracetobacillus of 10:7:3:3, zymomonas mobilis, Bifidobacterium, lactobacillus acidophilus.
Control experiment and result:
Mode according to embodiment 1, different strain fermentations is used to produce biology cellulose, remaining condition of culture is all identical, bacterial classification is respectively adopted the mixed fermentation bacterial strain being used alone gluconate pyracetobacillus and embodiment 1-4, the conversion ratio of reduced sugar in the biological fiber cellulose content obtaining after investigating identical fermentation time and fermentation medium, result is as shown in the table:
| Fermented bacterium | Yield of biological cellulose | The conversion ratio of reduced sugar in fermentation medium |
| Gluconate pyracetobacillus | 8.2g/L | 19% |
| The mixed fermentation bacterial strain of embodiment 1 | 14.9g/L | 37% |
| The mixed fermentation bacterial strain of embodiment 2 | 12.7g/L | 28% |
| The mixed fermentation bacterial strain of embodiment 3 | 13.4g/L | 31% |
| The mixed fermentation bacterial strain of embodiment 4 | 14.5g/L | 34% |
Can be drawn by upper table, the mixed fermentation bacterial strain of the present invention can effectively improve biology cellulose fermentation yield, improves conversion rate of reducing sugar.
Embodiment described above is only to be described the preferred embodiment of the present invention; not the scope of the present invention is defined; on the premise of designing spirit without departing from the present invention; this area ordinary skill technical staff technical scheme is made various deformation and improve, all should fall into claims of the present invention determine protection domain in.
Claims (10)
1. the mixed fermenting agent for biology cellulose fermentation, it is characterised in that: comprise gluconate pyracetobacillus, zymomonas mobilis and Bifidobacterium and/or lactobacillus acidophilus.
2. mixed fermenting agent according to claim 1, it is characterised in that: the ratio between described gluconate pyracetobacillus, zymomonas mobilis and Bifidobacterium and/or lactobacillus acidophilus is 10-20:7-15:3-8.
3. mixed fermenting agent according to claim 2, it is characterised in that: the ratio between described gluconate pyracetobacillus, zymomonas mobilis and Bifidobacterium and/or lactobacillus acidophilus is 17:11:5.
4. mixed fermenting agent according to claim 1, it is characterised in that: described bacterial strain uses front through activation culture.
5. mixed fermenting agent according to claim 2, it is characterised in that: the ratio between described bacterial strain is the volume ratio of the seed liquor obtaining that spreads cultivation again after activation.
6. a biology cellulose fermentation preparation, it is characterised in that: use the mixed fermenting agent described in any one of claim 1-5.
7. fermentation preparation according to claim 6, it is characterised in that: described fermentation temperature is 25-35 degree, and fermentation time is 3-10 days.
8. fermentation preparation according to claim 6, it is characterised in that: described fermentation is tray static fermentation or shaking flask or fermentation tank dynamic fermentation.
9. fermentation preparation according to claim 6, it is characterised in that: during described fermentation, pH value is between 6.2-7.2.
10. fermentation preparation according to claim 6, it is characterised in that: containing the carbon source decomposed for described bacterial strain in described fermentation culture medium.
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| CN201510138861.0A CN106148217B (en) | 2015-03-27 | 2015-03-27 | Mixed fermentation microbial inoculum for fermentation of biological cellulose |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110368315A (en) * | 2019-08-05 | 2019-10-25 | 衡欣实业有限公司 | A kind of probiotics conditioning foster skin facial mask and preparation method thereof |
| CN112021574A (en) * | 2020-08-12 | 2020-12-04 | 上海交通大学 | Preparation method and application of edible bacterial cellulose |
| CN113355377A (en) * | 2020-03-03 | 2021-09-07 | 株式会社现代百朗德 | Mixed bacteria cellulose facial mask with skin microbial population regulating effect and preparation method thereof |
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| CN103060229A (en) * | 2012-12-19 | 2013-04-24 | 华南理工大学 | gloconacetobacter xylinum and method for fermenting high-yield bacterial celluloses through mixed flora |
| CN104382751A (en) * | 2014-10-31 | 2015-03-04 | 天津科技大学 | Method for directly synthesizing bacterial cellulose/biological preservative composite mask by using fermentation process |
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2015
- 2015-03-27 CN CN201510138861.0A patent/CN106148217B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103060229A (en) * | 2012-12-19 | 2013-04-24 | 华南理工大学 | gloconacetobacter xylinum and method for fermenting high-yield bacterial celluloses through mixed flora |
| CN104382751A (en) * | 2014-10-31 | 2015-03-04 | 天津科技大学 | Method for directly synthesizing bacterial cellulose/biological preservative composite mask by using fermentation process |
Non-Patent Citations (1)
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| 李欣等: "细菌纤维素的合成与调控进展", 《生物加工过程》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110368315A (en) * | 2019-08-05 | 2019-10-25 | 衡欣实业有限公司 | A kind of probiotics conditioning foster skin facial mask and preparation method thereof |
| CN113355377A (en) * | 2020-03-03 | 2021-09-07 | 株式会社现代百朗德 | Mixed bacteria cellulose facial mask with skin microbial population regulating effect and preparation method thereof |
| CN112021574A (en) * | 2020-08-12 | 2020-12-04 | 上海交通大学 | Preparation method and application of edible bacterial cellulose |
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| CN106148217B (en) | 2023-12-19 |
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