CN106177509A

CN106177509A - Natural hypotensor medicine

Info

Publication number: CN106177509A
Application number: CN201510999847.XA
Authority: CN
Inventors: 赵文元
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-12-21
Filing date: 2015-12-21
Publication date: 2016-12-07

Abstract

Essential hypertension is the especially big difficult problem in world medicine field, the cerebral hemorrhage that especially this disease causes, and brings serious threat to the life of the mankind.Although miscellaneous chemicals can maintain the present situation of hypertension, but finally makes the pathological changes essence of this disease increase the weight of day by day.For curing hypertension, the present invention uses Chinese medicine classical theory, through basis and the clinical research of many decades, it was found that can effectively cure the natural Chinese medicinal herb of hypertension, such as Radix Puerariae, Ganoderma, Spica Prunellae etc..Utilize supercritical liquid extraction technique to extract the active ingredient of each taste Chinese medicine, make the natural hypotensor medicine of new Chinese medicine that can cure hypertension.Research proves, the mechanism that hypertension cured by this medicine is, can improve the activity of SOD by promoting the gene expression of SOD, remove free radical；Promote the gene expression of SOD, increase the generation of NO；The apoptosis of suppression endotheliocyte, improves and recovers the function of endotheliocyte, from substantially repairing the blood vessel of pathological changes, plays curative buck functionality.

Description

Natural hypotensor medicine

Natural hypotensor medicine belongs to Chinese medicine high-tech area.

The prescription composition of natural hypotensor medicine:

Radix Ginseng, Cornu Cervi Pantotrichum, Fructus Lycii, Radix Polygoni Multiflori, Rhizoma Gastrodiae, Ramulus Uncariae Cum Uncis, Cortex Lycii, Radix Puerariae, Ganoderma lucidum seu Japonicum, Radix Polygalae, Spica Prunellae, Semen Ginkgo Leaf, Flos Chrysanthemi, Poria, Radix Glycyrrhizae.

Producing of natural hypotensor medicine:

By clean for the sieve series respectively of 15 taste Chinese herbal medicine in prescription, 60 mesh pulverizers coarse powder respectively, it is respectively put into supercritical CO₂ Fluid Extractor.

The pressure of fluid extraction is most preferably 41mpa.

The optimum temperature of fluid extraction is 55 DEG C.

The Best Times of hydrodynamic extraction is 32 minutes.

Entrainer optimum in fluid extraction is ethanol.

Entrainer consumption in fluid extraction is 1/the 20 of overall extracted amount.

Dynamic fluid extracts 32 minutes, carries out interface extraction, proposes the effective elements of the medicine in each taste respectively, such as hypotensor medicine Element, panaquilon, linoleic acid, taurine, lycium barbarum polysaccharide.

The various Effective Component of Chinese Medicines of said extracted, are natural hypotensor medicine after mixing.

The basic research of natural hypotensor medicine curative effect

The essence of essential hypertension, is the dysfunction of vascular endothelial cell.Vivo oxidation stressed condition is lower to be produced Radical damage, be the important mechanisms causing Dysfunction of vascular endothlial cells.Free radical can inactivate NO^[1], suppress NOS Activity^[2], cause the generation of NO to greatly reduce, the synthesis of ET-1 is greatly increased with release, shows lasting contracting blood vessel effect Should, make essential hypertension be started and maintain.According to this theoretic knowledge, for effectively treating essential hypertension, will The natural Chinese medicinal herb of the cured essential hypertension that research finds, such as Radix Puerariae, Radix Polygalae, Ganoderma lucidum seu Japonicum^[3], apply supercritical fluid Abstraction technique, extracts the principle active component of each taste plant medicinal herbs, makes new Chinese medicine----natural hypotensor medicine, and through efficient liquid phase Color general technology purification, is made into certain density test medicinal liquid, carries out the Basic Experiment Study of original hypertensive rat.

One, materials and methods

1, laboratory animal:

Male original hypertensive rat (SHR), is purchased from Beijing profit China Experimental Animal Center.Body weight 200-220 gram, blood pressure (176.6±3.4)。

Key instrument: quantitative PCR apparatus (American AB I company)；Flow cytometer (U.S. company BD FACSC-alibur)；Thoroughly Penetrate ultramicroscope (Hitachi 7500 type)；RBP-1 type rat blood pressure meter (Institute for Medical Research of China-Japan Friendship Hospital)；Biochemistry analyzer Lisa 3000plus (France HYEL)；Microplate reader (U.S. Bio-Tec biotechnology Products)；

Main agents: reverse transcriptase polymerase chain reaction (RT-PCR) test kit, purchased from QIAGEN company of the U.S.；NO、 NOS, MDA, SOD test kit, is purchased from Nanjing and builds up Bioengineering Research Institute；ET-1 test kit, is had by the magnificent biological engineering in Beijing Limit company provides.

2, Experimental agents: Ganoderma lucidum seu Japonicum, is purchased from Beijing pharmacy of Tongrentang, extracts through supercritical liquid extraction technique, extraction pressure Power 30 kPas, extraction temperature 65 DEG C, entrainer is 60% ethanol, extraction time 60 minutes.Carried paste extract, through high-efficient liquid Phase chromatographic technique purification, after lyophilization, pulverizes and loads capsule, every 0.3g, be equivalent to crude drug in whole 6g.Take in capsule during experiment The tolerant distilled water that adds dissolves, and is made into the dosage of 60g/L.Positive control drug Captopril tablets, Changzhou pharmaceutical factory produces, lot number 312731, every 25mg, grind that add distilled water to be made into content be 2.5g/L into powder.

Two, experimental technique:

1. animal packet and treatment: male original hypertensive rat (SHR) 30, blood pressure (176.6 ± 3.4) mmHg, It is randomly divided into matched group, natural hypotensor medicine treatment group (being called for short natural hypotensor medicine group), positive drug captopril matched group (abbreviation Positive group).Each group SHR is on the basis of normal raising, and matched group gives the treatment of distilled water gavage, and natural hypotensor medicine group gives sky So hypotensor medicine 0.6g/kg gavage treatment, positive group gives captopril 0.03g/kg, 1 gavage treatment every day.

2. rat blood pressure is measured:

After treating 8 weeks, measure the blood pressure of SHR rat, replication 3 times.

3. biochemical indicator detection:

After each group rat treatment, 6h on an empty stomach, after anesthesia, left common carotid artery intubates and takes blood 5ml, centrifugal after take supernatant, according to Test kit illustrates, measures the content of NO, NOS, ET-1, SOD, MDA, and the change of blood fat.Carry out by Coomassie brilliant blue colorimetry Protein urine, measures the activity of SOD, NOS.

5. the mensuration of Molecular biology

Use Northern Blotting method, SABC, western blot and RT-PCR method detection SOD, NOS, The gene expression of HDL, with the apoptosis of Flow cytometry endotheliocyte.

4, statistical method:

Each group experiment the data obtained represents with (X ± S), uses SPSS17.0 statistical software to carry out the t inspection of Group Design And variance analysis, the difference between more each group.

Three. experimental result

1. the change of blood pressure:

After each group rat treats 8 weeks, the blood pressure of natural hypotensor medicine group rat tends to normal level, and matched group compares, P ＜ 0.01, and positive group compares, P ＜ 0.01.Dynamic property is observed visible, and natural blood pressure lowering have the effect strengthening rat physical ability.Concrete number According to being shown in Table 1.

The blood pressure (x ± S, mmHg) after rat is treated 8 weeks respectively organized by table 1

Note: natural hypotensor medicine group, positive component do not compare with matched group, #:P ＜ 0.05, ##:P ＜ 0.01；Natural blood pressure lowering Element group compares with positive group, *: P ＜ 0.05, * *: P ＜ 0.01.Lower same

2. serum NO level, the change of NOS, ET-1 after each group rat treatment:

The content of the NO of natural hypotensor medicine group rat significantly raises, and the level of ET-1 is remarkably decreased, and matched group compares, P ＜ 0.01.Detailed data is shown in Table 2.

The change (x ± s, n=10) of rat treatment front NO, NOS, NOS, ET respectively organized by table 2

3. the change of Antioxidant Indexes after each group rat treatment:

The content of natural hypotensor medicine group rat MDA is decreased obviously, and the activity of SOD substantially rises, and matched group compares, P ＜ 0.01, detailed data is shown in Table 3.

The change (x ± s, n=10) of MDA and SOD after rat is treated respectively organized by table 3

4. the testing result of gene expression after each group rat treatment:

The gene expression of natural hypotensor medicine group rat aorta SOD, NOS is substantially raised, and the gene expression of liver HDL is the brightest Show and raise, and matched group compares, P ＜ 0.01.The apoptosis rate of endotheliocyte is significantly reduced, and matched group compares, P ＜ 0.01.

Table 4 is respectively organized rat and is controlled the apoptosis rate (x ± s) of aorta posterior SOD, NOS, the change of liver HDL gene expression and EC

5. the change of blood fat after each group rat treatment:

After each group rat treats 8 weeks, the serum TC of natural hypotensor medicine group rat, TG, LDL the most substantially reduce, and the water of HDL Put down notable rising, and matched group compares, P ＜ 0.01.Detailed data is shown in Table 5.

The change (x ± s, n=10) of serum lipid metabolism after rat smelting is treated respectively organized by table 5

Discuss

Under the conditions of oxidative stress, produced free radical, is the important mechanisms of endothelial dysfunction, is that constitutional is high The essence of blood pressure.During hypertensive state, the growing amount of NO significantly reduces, and NO is internal important messenger molecule and effect is divided Son, for diastole, the transmission of nerve signal of blood vessel, plays important regulating and controlling effect^[4].NOS is the dominant of catalysis NO synthesis Matter^[5].Free radical can inactivate NO, and accelerates nitric oxide production degraded.Thus cause the generation of endothelial dysfunction, lead The synthesis and the release that cause ET-1 increase, and show lasting contracting vascular effect.Therefore, the study medication of hypertension, should be with Remove freely, increase the generation of NO, improving and recovering the function of endotheliocyte is target spot.

Internal too high LDL, can enter endotheliocyte, and stimulating endothelial cell produces active oxygen, and it can conduct as signal The second message,second messenger of path, the damage of inducing endothelial cell^[6], the activity of NOS in endotheliocyte can be suppressed again, and accelerate the fall of NO Solve, cause the apoptosis of endotheliocyte^[7].This result of study shows, in the gene expression of the SOD of natural hypotensor medicine group rat substantially Adjusting, activity is remarkably reinforced, and the content of MDA substantially reduces, and the apoptosis rate of endotheliocyte is significantly reduced simultaneously, and matched group ratio Relatively, difference is the most notable.Thus proving, natural hypotensor medicine can be effectively facilitated the gene expression of SOD, the content of enhancement of SOD With activity, remove free radical, reduce the generation of MDA, the apoptosis of endotheliocyte can be suppressed again, from antioxidation, the angle of anti-apoptotic, Improve and recover the thin function of endothelium, so that hypertension obtains the effective treatment.

NO and ET-1 is the active substance of a pair mutual antagonism of endothelial cells secrete.Under normal circumstances, both antagonisms Effect is among dynamic equilibrium, thus maintains normal diastolic and the contractile function of blood vessel, and the stablizing of blood pressure.Hypertension During state, the activity inhibited of NOS, the generation of NO greatly reduces, and causes the excessively synthesized of ET-1 and release.This active substance Level raise, show as lasting contracting vascular effect, promote the formation and development of hypertension further.This result of study table Bright, the gene expression of natural hypotensor medicine group rat NOS is substantially raised, and the generation of NO significantly rises, and the level of ET is remarkably decreased.By This proves, natural hypotensor medicine can be effectively facilitated the gene expression of NOS, increases content and the activity of NOS, promotes the generation of NO, Reduce the level of ET-1, improving endotheliocyte functionally, making blood pressure stabilization at normal condition.

LDL, in the subcutaneous deposition of Ink vessel transfusing, is the basis of response to oxidative stress, is the important initiating link of hypertension formation One of^[8], the reason of oxidation emergency, be lipid Developmental and Metabolic Disorder caused by.And HDL plays very important work in lipid metabolism With.The major function of HDL, be by blood plasma swashs from cholesterol change into cholesteryl ester, stop swash from cholesterol at blood vessel The deposition of wall, stops the generation of endothelial dysfunction.There are some researches prove, HDL, in the metabolic mechanism of lipid, shows as one Individual HDL molecule can transport 5--6 LDL molecule or TG molecule carries out catabolism to liver.But, due to the molecule body of HDL Long-pending the least, endarterium can be navigated within, take blood vessel wall out of by being deposited on the cholesterol inside endarterium, thus repaired blood Endothelial tube, has recovered the elasticity of blood vessel, makes the easypro contracting blood vessel that endotheliocyte is brought into normal play, the function regulating and controlling blood pressure.Therefore, regulation Or the level of rising blood plasma HDL, also it is an important directions for the treatment of essential hypertension.This result of study shows, natural fall The gene expression of pressure element group rat HDL is substantially raised, and its content is significantly raised, and the accumulation of LDL significantly reduces, and matched group ratio Relatively, there is obvious difference, it can be seen that, natural hypotensor medicine can regulate and control the gene expression of HDL exactly, improves the level of HDL, Accelerate the metabolism of cholesterol, reduce LDL and pile up, reduce the generation of response to oxidative stress, stop endothelial dysfunction to be formed With development, recover endotheliocyte vasodilator, the function of regulation and control blood pressure, make blood pressure tend to poised state.

List of references

1Tai MH, Wang LL, Wu kL, et.al.Increasd superoxid anion in rostral ventrolateral medulla contributes to hypertension in spontaneously Hypertensive rats via interactions wirh nitric oxide [J] .Fadic Biol Med, 2005, 38:450-462.

2 Li Jian. the effect [J] in oxidative stress arteriosclerosis disease generating process around.China's geriatrics magazine, 2007,26:328.

3Lee SY.Cardiovascular effects of mycelium extract of Ganoderma Lucidum:Inhibition of sympathelic outflow as a m echanism of its hypotensive Action [J] .Chem Pharm Bull, 1990,38:1395.

4Stoyanovo II, Lazarov NE.Localiztion of intric oxide synthase in rat trigeminal primary afferent neurons using NATPH-diaphorase histochmistry[J] Mol Histol, 2005,36 (3): 187.

5Britten MB Zeiher AM, Schahinger V.Chinical importance of coronary Endothelial vasodilator dysfunction and therapeutic options [J] .Intern Med, 1999,245:315-327.

6Pantoni L, Arteriosclerosis, thrombosis, and vascular [J] .Biology, 1998, 18:503-513.

7Borutaite V, Morkuniene R, Brown GC.Nitric oxide donors, nitrothils, and mitochondrial respiration inhibitora induce caspase activation by different Mechanisms [J] .Febs Lett, 2000,467 (2-3): 155-159.

8Labreuche J, Touboul PJ, Amarenco P.Plasma triglyceride levels and Risk of stroke and carotid atherosclerosis:a systematic review of the Epidemiological studies [J] .Atherosclerosis, 2009,203:331-345/

The clinical research of natural hypotensor medicine curative effect

Essential hypertension, closely related with arteriosclerosis, it is one of ten hang-ups in world medicine field.The world Health organization statistics show, the positive cumulative year after year of sickness rate of this disease, in the crowd after the mankind are adult, and existing 1/3rd Crowd, suffer from essential hypertension.The sickness rate of hypertension is the highest, and, the lethal disability rate also subsidiary factory of this disease shies People, is apoplexy and the half of deaths from heart disease number.Therefore, hypertension has become the serious problems of harm human health.Doctor trained in Western medicine Medicine treatment hypertension generally requires drug combination and can be only achieved antihypertensive effect.And the untoward reaction of medicine, instead result in this disease Pathogenesis increases the weight of day by day.Therefore, hypertension prevented and treated by application Chinese medicine, then seem the most necessary.To this end, this research summary is for many years Clinical experience, certainly draws up ERLONG one tiger blood pressure lowering side, and through supercritical liquid extraction technique, makes hypotensor medicine granule, through clinic The hundreds of example of Coryza Treated by Syndrome Differentiation primary hypertension patient, all obtains prominent original curative effect, and concrete research method and result are as follows.

1, clinical data

1.1 physical data

200 example Essential Hypertensive Patients, from this Out-patient Department in May 2012 June in 2009, use Dool ' s to face Bed case random table, is randomly divided into two groups by patient, treatment group 100 example, middle male 70 example, women 30 example, the age 27 71 years old, the mean age (51.3 ± 10.5), course of disease 1-25, average course of disease (11.6 ± 4.5) year.Mean blood pressure 168.3 ± 12.5/93.6 ± 8.9mmHg (1mmHg=0.133kpa).Matched group 100 example patient, male 60 example, women 40 example, age 28- 68 years old, average (58.2 ± 10.6) year.Course of disease 1-28, average course of disease (10.6 ± 6.5), mean blood pressure (158.8 ± 13.6) (93.6 ± 8.8) mmHg, the Baseline Data of upper two groups of patients compares, and diversity not statistically significant (p ＞ 0.05), having can Compare property.

1.2 diagnostic criteria

Western medicine diagnostic criteria employing " China's hypertension prevention and control guide " [1] standard, contraction pressure (SBP) of being i.e. grown up >= 140Hg, diastolic pressure (DBP) >=90mmHg.Chinese medicine diagnostic criteria, according to the liver in " new Chinese medicine clinical guidance principle " [2] Sun rising, the diagnostic criteria of type of deficiency of YIN leading to hyper activity of YANG.

1.3 inclusive criteria

1. the western medicine diagnostic criteria of essential hypertension is met.

2. the CM syndrome differentiation criterion of Chinese medicine excessive rising of liver-YANG, hyperactivity of YANG due to deficiency of YIN is met.

3. the age of hyperpietic is in 27-71 year.

4. obtain the informed consent of each patient, be provided simultaneously with above 4 condition persons and can include test in.

1.4 row's listing standards

1. SBP ＞ 225mmHg and the primary hypertension patient of DBP ＞ 125mmHg.

2. secondary hypertension patient

3. gestation or breast-feeding female.

4. the severe primary such as cerebral hemorrhage, left heart failure, renal failure and other hearts, brain, liver and hemopoietic system it are associated with Sick patient.

5. psychotic, allergic constitution and other antihypertensive drugs person can not be disabled.

Do not take medicine by regulation, it is impossible to judge curative effect or data is congruent affects the treatment and safety judgement person.

2. research method

2.1 Therapeutic Method

Treatment group: take natural hypotensor medicine, natural hypotensor medicine is divided into excessive rising of liver-YANG and hyperactivity of YANG due to deficiency of YIN two kinds.

1. the prescription of liver-Yang sthenia type: Rhizoma Gastrodiae 6g, Os Draconis 15g, Concha Haliotidis 15g, Radix Scrophulariae 6g, Radix Paeoniae Alba 6g, Flos Chrysanthemi 6g, Radix Gentianae Grass 3g, Radix Scutellariae 6g, Radix Rehmanniae 6g, Radix Bupleuri 6g, Radix Angelicae Sinensis 6g, Radix Salviae Miltiorrhizae 6g, Poria 6g, Radix Glycyrrhizae 3g.

2. the prescription of type of deficiency of YIN leading to hyper activity of YANG: Rhizoma Gastrodiae 6g, Os Draconis 15g, Radix Scrophulariae 6g, Radix Paeoniae Alba 6g, Flos Chrysanthemi 6g, vinegar Carapax et Plastrum Testudinis 10g, Fructus Lycii Sub-6g, Radix Polygoni Multiflori Preparata 10g, Rhizoma Polygonati 10g, Radix Achyranthis Bidentatae 6g, Flos Carthami 3g, Radix Rehmanniae Preparata 6g, Radix Glycyrrhizae 3g.

Above medicine all extracts with water logging precipitate with ethanol technology and makes pill, each serving 3g, takes 2 every day.Various patient respectively reports Various medication.

Matched group: take Shanxi Jin Huayun star pharmaceutical Co. Ltd produce Captopril tablets, every 25mg, every day everyone Take 3 times.

Two groups of each continuous treatments of patient 6 weeks, observe curative effect.All with less salt, low fat diet during treatment, disable other falls Pressure, blood fat reducing and affect the medicine of blood pressure.Other serious change person such as occurs during treatment, test of cure must be exited.

2.2 observation index and method

1. the mensuration of blood pressure: use the sphygomanometer of ticket people's mercury column, seat measures blood pressure 3 times, record result of averaging, often Week at least measures 3 times.

2. clinical symptoms improves situation: include that brain is dizzy, headache, impetuous irritability, waist soreness, dysphoria with feverish sensation in the chest palms and soles, cardiopalmus, mistake Dormancy, dreaminess, flushing conjunctival congestion, dry mouth and tougue, tinnitus, forgetful, constipation, urinate red.

3. check respectively for blood before and after treatment and defecation is conventional, and hepatic and renal function, blood fat and electrocardiogram.

2.3 criterion of therapeutical effect

2.3.1 efficacy of antihypertensive treatment criterion

According to the blood pressure curative effect determinate standard evaluation result in " new Chinese medicine guideline of clinical investigations tries " [2].

Effective: DBP declines more than 10mmHg, and reaches normal arterial pressure scope, though or DBP do not drop to normal range, but Blood pressure has declined 20mmHg or above person.

Effective: although DBP decline does not reaches 10mmHg after treatment, but to have reached also scope, or DBP relatively treats front decline 10-19mmHg, but not up to normal arterial pressure scope, or SBP relatively treat before decline below 30mmHg, must each wherein 1 standard of tool.

Invalid: after treatment, blood pressure is not up to above criterion of therapeutical effect.

2.3.2 individual event symptom curative effect determinate standard

Effective: after treatment, original symptom is wholly absent or significantly alleviates.

Effective: after treatment, original symptom has taken a turn for the better or has alleviated.

Invalid: after treatment, original symptom has no improvement.

2.4 statistical method

This research application SPSS16.0 software kit, carries out data statistic analysis.Enumeration data uses X²Inspection, measurement data Using seven inspections, P ＜ 0.05 represents that difference has statistical significance.

3 results

3.1 liang of group symptom comparitive study

In addition to tinnitus, forgetful symptom group difference not statistically significant (P ＞ 0.05), remaining symptom group difference all has Statistical significance (P ＜ 0.05), treatment group symptom is improved and is better than matched group, the results are shown in Table 1

1 liang of table group primary hypertension patient symptom comparitive study (example)

Note: compare with matched group, * P ＜ 0.05

After 3.2 liang of group primary hypertension patient treatments, the comparison of efficacy of antihypertensive treatment, is shown in Table 2.

The comparison (example) of 22 groups of essential hypertensions of table

Note: two groups are compared, P ＜ 0.05.

Before and after 3.3 liang of group primary hypertension patient treatments, the change of blood pressure is compared, and is shown in Table 3

Before and after the group primary hypertension patient treatment of 3 liang of table, blood pressure compares (example)

Group	Time	Number of cases	SBP	DBP
					Treatment group	Before controlling	100	161.1±13.5	96.3±9.6
	After controlling	100	117.3±6.8^※Δ	78.2±2.8^※Δ
					Matched group	Before controlling	100	162.2±14.6	95.1±9.6
	After controlling	100	143.5±4.9^※	88.6±2.3^※

Note: compare before controlling with this group, ※ P ＜ 0.05；Compare after controlling with matched group, Δ P ＜ 0.05

Before and after 3.4 liang of group primary hypertension patient treatments, the change of heart rate is compared

Treatment group average heart rate treatment before (78.3 ± 12.5) secondary/min, the heart rate after treatment be (76.1 ± 12.6) secondary/ min；Before treatment of control group (78.1 ± 11.8) secondary/min, (76.4 ± 13.3) secondary/min.Compare before and after two groups of treatments and between group, The equal not statistically significant of difference (P ＞ 0.05)

Before and after 3.5 liang of group essential hypertension treatments, the change of blood fat is compared

After treatment, treatment group T-CHOL (TC), triacylglycerol (TG), HDL-C (HDL-C), all have Significantly improve, P ＜ 0.05, and matched group is only TG1 item is significantly improved, P ＜ 0.05.Detailed data is shown in Table 4.

Before and after 42 groups of Essential Hypertensive Patients treatments of table, Blood Lipid compares (example)

Note: and compare before the treatment of this group.※ P ＜ 0.05；Compare after controlling with matched group, Δ P ＜ 0.05

3.6 untoward reaction

Treatment group patient is without the discomfort of subjectivity.The blood of two groups of patients, urine, liver function, renal function, electrocardiogram inspection before and after treatment Look into and be showed no ANOMALOUS VARIATIONS (P ＞ 0.05).

4, discuss

The study find that, it is the most high that essential hypertension mostly is in early days flaming up of liver-fire, liver-yang, after mid-term, gradually develops into The hepatic and renal YIN deficiency, occurs that hyperactivity of YANG due to deficiency of YIN is main disease.It is said that in general, the patient of liver-Yang sthenia type, the age is relatively light, and the course of disease is shorter, And the patient of type of deficiency of YIN leading to hyper activity of YANG, older, the course of disease is longer, often simulataneous insufficiency and excessive in pathogenic process, and many constitutionales Hyperpietic, is often accompanied by lipid metabolic disorder^[4].But, natural hypotensor medicine is mainly with Rhizoma Gastrodiae, Os Draconis, Flos Chrysanthemi etc., with suppressing the hyperactive liver YANG hyperactivity suppressing；With Spica Prunellae, Poria, the Radix Rehmanniae etc., and purging liver-fire；With Fructus Lycii, Cornu Cervi Pantotrichum, Stigma Croci etc., with nourishing the liver and kidney.According to card Type combines, respectively treatment excessive rising of liver-YANG and the essential hypertension of the big type of hyperactivity of YANG due to deficiency of YIN two.Rhizoma Gastrodiae in side, nature and flavor are sweet flat, There is the merit of suppressing the hyperactive liver to relieve the wind syndrome.And Ramulus Uncariae Cum Uncis nature and flavor are sweet cool, it may have the merit of clearing away heat and relieving the wind syndrome.Spica Prunellae nature and flavor Cheng Han in side, has Purging liver-fire, effect of suppressing the hyperactive liver to relieve the wind syndrome, three is combined into monarch drug, and other all medicines use, plays suppressing the hyperactive liver and subsiding YANG, nourishing YIN to lower pathogenic fire, tune altogether With QI and blood, the effect that adjusts the dual power of negative and positive principles.Modern pharmacological research shows, Ramulus Uncariae Cum Uncis, Rhizoma Gastrodiae, Flos Chrysanthemi, Poria, Radix Polygalae, Spica Prunellae, Radix Polygoni Multiflori Deng, it is respectively provided with obvious hypotensive effect, Radix Polygoni Multiflori, Fructus Lycii, Flos Chrysanthemi etc., also there is the function of antioxidation, blood fat reducing, have again guarantor Protect the effect of endotheliocyte^[5-6].This result of study shows, natural hypotensor medicine has hypotensive effect significantly, and can effectively change Kind and eliminate the clinical symptoms of essential hypertension, the most also there is original blood pressure lowering, high density lipoprotein increasing, and weight The protection wanted and reparation blood vessel endothelium function carefully.

5, list of references

1, China's hypertension prevention and control guide revision committee, China's hypertension prevention and control guide (revised edition) [M], Beijing, people People health publishing house, 2005.

2, Zheng Xiao Huang new Chinese medicine guideline of clinical investigations (trying) [S].

Beijing: China Medical Science Press, 2002:73-77

3, Tang Shihuan, Yang Hongjun, yellow fine jade fine jade etc., tcm clinical practice prescription decoction pieces consumption investigation report (internal medicine) [J], in China Medicine magazine, 20083,33 (19): 2257-2263.

Claims

The most natural hypotensor medicine belongs to modern Chinese medicine sciemtifec and technical sphere, the prescription of this medicine, is made up of pure natural Chinese herbal medicine, it may be assumed that Radix Ginseng, deer Young pilose antler, Fructus Lycii, Radix Polygoni Multiflori, Rhizoma Gastrodiae, Ramulus Uncariae Cum Uncis, Cortex Lycii, Radix Puerariae, Ganoderma lucidum seu Japonicum Radix Polygalae, Spica Prunellae, Folium Ginkgo, Flos Chrysanthemi, Poria, sweet Grass.Each medicine in side, utilizes supercritical liquid extraction technique to extract, then with high-efficient liquid phase chromatogram technology purification, by lyophilization Make granule, named natural hypotensor medicine.Numerous studies prove, natural hypotensor medicine can improve by promoting the gene expression of SOD SOD activity, removes free radical；Promote the gene expression of NOS, strengthen the activity of NOS, increase the generation of NO；Suppression endotheliocyte Apoptosis, regulation and control HDL gene expression, improve HDL level, thus improve and recover endotheliocyte function, from substantially Effectively play buck functionality, solve the world-famous puzzle that essential hypertension is difficult to cure.
2. forming according to the prescription described in claims 1, it is mainly characterized by, and the party is with monarch, minister, the rule helping, make, institute's group Becoming, its function is QI and blood regulating, adjusts the dual power of negative and positive principles, saprophytic new, the hearing-improing and eyesight improving of suppressing the hyperactive liver to relieve the wind syndrome, activating blood circulation to dissipate blood stasis, change, make light of one's life by commiting suicide resistance to always.
3. according to described in claims 1 it: " Scavenger of ROS ", it is mainly characterized by, and main side's medicine of natural hypotensor medicine is Ganoderma lucidum seu Japonicum, and the raw remote, thickly forested mountains of Ganoderma lucidum seu Japonicum, the anima in the poly-world, the elite of long-pending all things on earth, for successive dynasties health care, make light of one's life by commiting suicide resistance to old it Good merchantable brand.Its main active ingredient---ganoderan, unique Scavenger of ROS, the merit of prevention free radical generation, repair the blood of pathological changes Pipe, is that essential hypertension recovers normal.
4. according to described in claims 1；" strengthening the activity of superoxide dismutase ", it is mainly characterized by, superoxides discrimination Change enzyme as one of internal important antioxidase, can Scavenger of ROS effectively.Active oxygen is that research is old and feeble and opposing is old and feeble Predominantly detect index.Research proves, natural hypotensor medicine can promote the gene expression of superoxide dismutase, strengthens superoxides The activity of dismutase, is the important function mechanism of natural hypotensor medicine scavenging capacity oxygen-derived free radicals.
The most according to claim 1；The apoptosis of suppression endotheliocyte, thus improve and recover the function of endotheliocyte, from reality Effectively play buck functionality in matter, solve the world-famous puzzle that essential hypertension is difficult to cure.
6. according to described in claims 1: " removing and stop the formation of free radical ", it is mainly characterized by；Free radical is body The harmful substance produced in Antioxidation reaction, has strong oxidizing property, can damage internal tissue and cell, and then cause cell Apoptosis and the aging of human body.Research proves, natural hypotensor medicine is possible not only to antioxidation, still can effectively stop the generation of free radical And stop the aging of cell.
7. according to described in claims 1；Promote the gene expression of NOS, increase the generation of NO, be to improve vascular endothelial cell merit The basis of energy.Being mainly characterized by, NO is internal important messenger molecule and effector molecule, for the diastole of blood vessel, nerve signal Transmission, plays important regulating and controlling effect^[4].NOS is the main matter of catalysis NO synthesis^[5].Free radical can inactivate NO, and accelerates Nitric oxide production degraded.Thus cause the generation of endothelial dysfunction, cause the synthesis of ET-1 and release to increase, show Lasting contracting vascular effect.Therefore, the study medication of hypertension, should improve and extensive to remove freely, to increase the generation of NO The function of multiple endotheliocyte is target spot.
8. according to described in claims 1；The apoptosis of suppression endotheliocyte, thus improve and recover the function of endotheliocyte, from The most effectively play buck functionality.It is mainly characterized by, and natural hypotensor medicine can make the gene expression of SOD substantially raise, and lives Property is remarkably reinforced, and the content of MDA substantially reduces, and the apoptosis rate of endotheliocyte is significantly reduced simultaneously, and matched group compares, difference The most notable.Thus proving, natural hypotensor medicine can be effectively facilitated the gene expression of SOD, the content of enhancement of SOD and activity, Remove free radical, reduce the generation of MDA, the apoptosis of endotheliocyte can be significantly inhibited again, from antioxidation, the angle of anti-apoptotic, change It is apt to and recovers the thin function of endothelium, so that hypertension is preferably cured.
9. according to described in claims 1: " strengthening the activity of nitric oxide synthetase ", it is mainly characterized by comprising: that nitric oxide closes Become enzyme to be widely present in endotheliocyte, neurocyte, L-arginine can be catalyzed and generate nitric oxide, in the merit maintaining endotheliocyte Can and stop cell aging on play particularly important effect.This enzyme can only be in precious species such as Solenognathus, protamine Can propose, research shows, natural hypotensor medicine can be effectively promoted the gene expression of nitricoxide synthase, improves containing of this enzyme Amount.
10. according to described in claims 1: " raising nitric oxide production level ", it is mainly characterized by comprising: nitric oxide, and this is important Informational molecule, the physiological function of modulating vascular plays the most crucial effect.So, the height of nitric oxide level Low, decide the degree of aging of cell, raise the key that nitric oxide production level is delaying cell aging.Research shows, natural Hypotensor medicine can significantly raise nitric oxide production level, the vigor to maintenance cell, plays the effect that can not be substituted.
11. according to described in claims 1: " natural hypotensor medicine can regulate and control the gene table of high density lipoprotein (HDL) exactly Reach, improve the level of HDL ", it is mainly characterized by, and natural hypotensor medicine can regulate and control the gene expression of HDL exactly, improves HDL's Level, accelerates the metabolism of cholesterol, reduces LDL and piles up, reduce the generation of response to oxidative stress, stops endothelial dysfunction Formed and development, recover endotheliocyte vasodilator, the function of regulation and control blood pressure, make blood pressure tend to poised state.