CN106173853B - Nutritional composition for reducing blood fat and application thereof - Google Patents
Nutritional composition for reducing blood fat and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/02—Peptides of undefined number of amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention belongs to the field of production of nutritional foods, and particularly discloses a nutritional composition for reducing blood fat, which comprises the following components in percentage by mass: 40-60% of cured cereal powder and maltodextrin, 15-35% of trachinotus ovatus peptide powder and skimmed milk powder, 10-20% of fruit and vegetable powder and 2-5% of sodium carboxymethylcellulose; the trachinotus ovatus peptide powder is obtained by performing enzymolysis, centrifugation, ultrafiltration and drying on trachinotus ovatus. The cured grain powder and the fruit and vegetable powder provided by the invention are rich in dietary fiber, and the sodium carboxymethylcellulose can further enhance the colloidal system of the dietary fiber in the intestinal tract, and can be compounded with the trachinotus ovatus peptide powder, so that the hepatic and intestinal circulation of bile acid is obviously reduced, the discharge of the bile acid along with excrement is increased, and the purpose of reducing blood fat is achieved. The nutritional composition can be used as health food, formula food with special medical application and common food, and is suitable for sub-healthy people with hyperlipidemia, who are busy in work and have a short life rhythm.
Description
Technical Field
The invention belongs to the technical field of nutritional foods, and particularly relates to a nutritional composition for reducing blood fat and application thereof.
Background
Hyperlipidemia is a common cardiovascular disease caused by disorder of blood lipid metabolism, and its main symptoms include elevation of Total Cholesterol (TC), total Triacylglycerol (TG), low-density lipoprotein cholesterol (LDL-c), and lowering of high-density lipoprotein cholesterol (HDL-c) in serum. The occurrence of hyperlipidemia is closely related to personal daily work and rest, especially improper dietary habits, besides genetic factors. The excessive high content of blood fat can further cause blood viscosity to induce various cardiovascular diseases, and hypertension and diabetes are also complications of hyperlipidemia, thereby causing great harm to human health. Dysregulated blood lipids are considered to be the basis for primary and secondary prevention of cardiovascular disease, significantly reducing the risk of death from cardiovascular disease. The chemical hypolipidemic drugs used clinically at present have definite curative effects, but have large side effects and high recurrence rate.
At present, researches find that the food-derived active substances can achieve the aim of assisting in reducing blood fat by a method of promoting bile acid secretion and reducing bile acid absorption. The food therapy method is used for assisting in reducing blood fat, and is safe and harmless, so that the food therapy method is more and more popular among doctors and patients. The method is based on the fact that about 40-50% of cholesterol in the human body is metabolically decomposed by the synthesis of bile acids. When the components in the food can combine with the bile acid to take away and discharge more bile acid in the intestinal tract after the food is digested, more cholesterol in the blood can be promoted to synthesize the bile acid to maintain the normal digestion and absorption of lipid food, thereby reducing the content of cholesterol in the blood and the body.
According to the existing research reports, the functional components with the function of assisting in reducing blood fat in food mainly comprise dietary fibers, bioactive peptides, fatty acids, phytosterols, phenols, polysaccharides and the like.
Among them, bioactive peptides are one of the most popular fields of research in the current functional food industry. The peptides, especially some oligopeptides, have the function of regulating the physiological function of the human body and have important application prospect in the food for assisting to reduce blood fat. At present, the research and development of the hypolipidemic effect of bioactive peptides at home and abroad are mainly focused on bean protein peptides and glycoprotein peptides, and the research on the preparation of the hypolipidemic peptides from marine fish proteins is rarely reported at home and abroad.
Bioactive peptides of marine origin have more broadly distinct biological activities due to differences in their amino acid composition and sequence from that of terrestrial organisms. At present, research on the aspect of reducing blood fat peptide of marine organisms mainly focuses on mollusks such as jellyfish collagen peptide.
The effect of dietary fiber on human health is more and more concerned by the world, and the dietary fiber also has obvious effect on reducing blood fat. The dietary fiber has active groups and physical properties of a colloidal system, so that the dietary fiber can adsorb cholesterol and cholate in intestinal tracts, and the formed colloidal system can prevent bile acid and cholesterol from migrating and absorbing to intestinal mucosa, thereby achieving the purpose of reducing bile acid liver and intestine circulation.
The invention aims to obtain the nutrient product for reducing the hyperlipidemia by combining the means, so that the nutrient product is used for people with demands, and the remarkable effect of assisting in reducing the hyperlipidemia is achieved.
Disclosure of Invention
The invention aims to provide a nutritional composition for reducing blood fat according to the defects in the prior art.
The invention also aims to provide the application of the nutritional composition in preparing hyperlipidemia medicines, health-care foods, special medical application formula foods and common foods.
The purpose of the invention is realized by the following technical scheme:
the invention provides a nutritional composition for reducing blood fat, which comprises the following components in percentage by mass:
40-60% of cured cereal powder and maltodextrin,
15-35% of trachinotus ovatus peptide powder and skimmed milk powder,
10-20% of fruit and vegetable powder
2-5% of sodium carboxymethylcellulose;
the trachinotus ovatus peptide powder is obtained by carrying out enzymolysis on trachinotus ovatus, centrifuging, filtering and drying.
The invention prepares oval pompano into small molecule peptide powder, which is different from marine animal peptide disclosed in the prior art, for example, a patent of 'a tube feeding clinical nutriment with hypolipidemic effect and a preparation method thereof' (CN 103637212A) discloses that sea bass is used as a raw material to prepare a clinical nutriment preparation with the hypolipidemic effect, but compared with the patent, the invention has different raw materials (ocean oval pompano) and different food populations (sub-healthy common population which can be eaten independently) and most importantly has different action mechanisms, namely the invention utilizes oval pompano peptide to be combined with other components to play a role of reducing blood lipid by blocking liver and intestine circulation of bile acid, and the action mechanism of the patent (CN 103637212A) mainly plays a role of reducing blood lipid by regulating an enzyme system in vivo.
Preferably, the content of the cured cereal powder and the maltodextrin in the cured cereal powder is 9-95%, and the content of the oval pompano peptide powder in the oval pompano peptide powder and the skimmed milk powder is 1-95%.
More preferably, the content of the cured cereal powder and maltodextrin in the cured cereal powder is 60-91%, and the content of the oval pompano peptide powder in the oval pompano peptide powder and the skimmed milk powder is 40-80%.
More preferably, the weight ratio of the cooked cereal powder to the trachinotus ovatus peptide powder is (2.0-3.5): 1.
in the invention, the dietary fiber and the trachinotus ovatus peptide powder have synergistic effect, and are combined with cholate in intestinal tracts to slow the reabsorption of the cholate, thereby jointly playing a role in reducing blood fat.
Preferably, the proportion of the protein hydrolysate of the trachinotus ovatus peptide powder with the relative molecular weight of less than 3000Da is more than 95%.
Preferably, the cooked cereal flour is comprised of one or more of cooked oat flour, cooked corn flour, cooked rice flour, cooked black rice flour.
More preferably, the cooked cereal flour comprises cooked oat flour and cooked corn flour in a weight ratio of cooked oat flour to cooked corn flour of 5: 2.
preferably, the preparation of the trachinotus ovatus peptide powder comprises the following steps:
s1, adding 1 time of water into trachinotus ovatus meat and smashing;
s2, adding 2000-6000U/g protease into the crushed trachinotus ovatus in the S1, hydrolyzing for 2-6 hours, inactivating enzyme at 95 ℃, cooling to 4-10 ℃, and centrifuging;
s3, taking the supernatant obtained after centrifugation in the S2, filtering the supernatant through a 10,000-3,000 MWCO ultrafiltration membrane, and drying the filtrate to obtain the trachinotus ovatus peptide powder.
Preferably, in S2, the protease is one or more of pepsin, trypsin, alkaline protease and papain.
Preferably, the separation factor F in the centrifuger≥ 800。
The cured grain powder and the fruit and vegetable powder provided by the invention are rich in dietary fiber, and the sodium carboxymethylcellulose can further enhance the colloidal system of the dietary fiber in the intestinal tract, and can be compounded with the trachinotus ovatus peptide powder to generate a synergistic interaction effect, so that the hepatic and intestinal circulation of bile acid is obviously reduced, the discharge of the bile acid along with excrement is increased, and the purpose of reducing blood fat is achieved. And after being compounded, the components of the invention have synergistic effect obviously stronger than the independent effect of the components.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the nutritional food prepared from the nutritional composition provided by the invention can reduce the content of Total Cholesterol (TC), total Triacylglycerol (TG) and low-density lipoprotein cholesterol (LDL-c) in serum, and remarkably improve the content of high-density lipoprotein cholesterol (HDL-c) and total cholesterol (FTC) in excrement, has a remarkable blood fat reducing effect, has a great application prospect when being used for preparing hyperlipidemia medicines, health-care foods, special medical purpose formula foods and common foods, and is particularly suitable for hyperlipidemia sub-health people with busy work and short life rhythm.
Drawings
FIG. 1 is a schematic diagram of the molecular weight distribution of Trachinotus ovatus peptide powder in example 1.
FIG. 2 is a schematic diagram of the molecular weight distribution of Trachinotus ovatus peptide powder in example 10.
FIG. 3 is a graph of the effect of gavage samples on serum TC levels in rats.
FIG. 4 is a graph of the effect of gavage samples on rat serum TG levels.
FIG. 5 is a graph of the effect of gavage samples on rat serum LDL-c levels.
FIG. 6 is a graph of the effect of gavage samples on rat serum HDL-c levels.
FIG. 7 is a graph of the effect of gavage samples on total cholesterol levels (FTC) in rat feces.
Detailed Description
The present invention will be further described with reference to the following specific examples and drawings, which are not intended to limit the invention in any manner. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the present invention are commercially available.
Example 1:
the nutritional food with the function of assisting in reducing blood fat comprises the following components in parts by weight:
350 parts of cured cereal powder (the ratio of the cured oat powder to the cured corn powder is 5: 2),
200 parts of maltodextrin, namely 200 parts of maltodextrin,
150 parts of skimmed milk powder, 150 parts of milk powder,
165 parts of fruit and vegetable powder, namely,
100 parts of trachinotus ovatus blood fat reducing peptide powder,
and 35 parts of sodium carboxymethyl cellulose.
The preparation method of the nutritional food with the function of assisting in reducing blood fat comprises the following steps:
s1 preparation of trachinotus ovatus lipopeptide reducing blood lipid: extracting Trachinotus ovatus meat, adding 1 times of deionized water, mixing, mincing, hydrolyzing with 4000U/g trypsin for 3 hr, inactivating enzyme at 95 deg.C, cooling to 5 deg.C, and centrifuging (separation factor F)rNot less than 800) removing oil and impurities, filtering the clear liquid with 3,000MWCO ultrafiltration membrane, drying the trapped liquid to obtain Trachinotus ovatus blood lipid-reducing peptide powder, wherein the molecular weight distribution of the peptide powder is shown in attached table 1 and attached figure 1, and the proportion of the relative molecular weight of protein hydrolysate is less than 3000Da is more than 95%;
s2, preparing materials: uniformly mixing the raw materials according to the proportion;
s3, packaging: and (6) filling nitrogen and sealing.
The molecular weight distribution of the trachinotus ovatus lipid-lowering peptide powder prepared in the S1 is shown in a figure 1 and a table 1:
table 1: molecular weight distribution of trachinotus ovatus peptide powder obtained before and after ultrafiltration treatment
Example 2
The ratio of cooked cereal powder (cooked oat powder and cooked corn powder is 5: 2) is increased to 500 parts, the ratio of maltodextrin to 50 parts, the ratio of skimmed milk powder to 50 parts, the ratio of trachinotus ovatus to blood fat reducing peptide to 200 parts, and the other components are the same as in example 1.
Example 3
100 parts of cured cereal powder (the ratio of the cured oat powder to the cured corn powder is 5: 2), 500 parts of maltodextrin, 50 parts of skimmed milk powder, 200 parts of trachinotus ovatus blood fat reducing peptide, and the other components are the same as in example 1.
Example 4
The ratio of the cooked cereal powder (cooked oat powder and cooked corn powder is 5: 2) is 50 parts, the ratio of the maltodextrin is 500 parts, and the other components are the same as the components in the example 1.
Example 5
The ratio of the cooked cereal powder (cooked oat powder and cooked corn powder is 5: 2) is 520 parts, the ratio of the maltodextrin is 30 parts, and the other components are the same as the components in the example 1.
Example 6
The weight parts of skimmed milk powder are 220, the weight parts of trachinotus ovatus hypolipemic peptide are 30, and the other components are the same as those in example 1.
Example 7
20 parts of skimmed milk powder, 230 parts of trachinotus ovatus hypolipemic peptide and the same components as in example 1.
Example 8
50 parts of cured cereal powder (the ratio of the cured oat powder to the cured corn powder is 5: 2), 500 parts of maltodextrin, 220 parts of skim milk powder, 30 parts of trachinotus ovatus blood fat reducing peptide powder and the other components in parts by weight are the same as in example 1.
Example 9
500 parts of cooked cereal powder (the ratio of the cooked oat powder to the cooked corn powder is 5: 2), 50 parts of maltodextrin, 20 parts of skimmed milk powder, 230 parts of trachinotus ovatus blood fat reducing peptide powder and the other components in parts as in example 1.
Example 10
In the step S1, the trachinotus ovatus peptide powder is directly dried by ultrafiltration to obtain powder, the molecular weight distribution of the obtained powder is shown in fig. 2 and table 1, and the other component proportions and treatment methods are the same as those in example 1.
The animals tested for reducing blood lipid in examples 1-10 and the positive control cholestyramine were divided into 13 groups of 6 animals each, and the test was repeated three times. The normal control group is fed with basal feed; the hyperlipemia model building group is used for feeding high-fat feed (high-fat feed: 52.2% of basal feed, 20% of cane sugar, 15% of lard, 10% of casein, 1.2% of cholesterol, 0.6% of calcium hydrophosphate, 0.4% of stone powder, 0.4% of premix and 0.2% of sodium cholate); the positive control group was given high-fat diet and gavage cholestyramine (0.2 g/kg. d) daily; high fat diet was administered to each of the groups 1 to 10, and the formula (2.0 g/kg. d) was gavaged each day.
After feeding for 70 days, fasting is carried out for 12h, and orbital blood is taken to separate serum for detecting TC, TG, LDL-C and HDL-C.
3 days before the test is finished, 3 days of test rat feces are collected, freeze-dried and combined to detect the cholesterol content FTC in the feces.
The test results are shown in Table 2 and FIGS. 3 to 7.
The test results show that, compared with the hyperlipidemia animal model group, the examples 1 to 3 can reduce the levels of Total Cholesterol (TC), total triglyceride and low-density lipoprotein cholesterol (LDL-c) in the serum of the experimental rat, improve the level of high-density lipoprotein cholesterol (HDL-c) in the serum and improve the content of cholesterol (FTC) in the feces. Among them, examples 1 and 2 can significantly reduce the levels of TC and LDL-c in rat serum, up-regulate the level of HDL-c, and significantly increase the FTC content in rat feces, showing the effect of promoting cholesterol excretion from the body. Moreover, the experimental data show that the effects of the examples 1 and 2 on reducing serum TC and increasing HDL-c level and FTC level are more obvious than that of the positive control medicament cholestyramine. Further, example 2 is superior to other examples in down-regulating TC, HDL-c and increasing FTC. In examples 7 and 9, due to the slightly high content of the peptide powder of the trachinotus ovatus, although the levels of TG and LDL-c in the serum of rats are higher than those in examples 1 to 3, the effect of reducing TC and FTC in the serum is inferior to that in examples 1 to 3, and the remarkable effect of reducing blood fat cannot be achieved. It can be seen from the experimental data that when the composition ratio of one or both of the cooked cereal powder and the trachinotus ovatus hypolipidemic peptide is lower than a certain range (for example, examples 4, 6 and 8), the effects on TC, TG, LDL-c, HDL-c in the serum of the experimental animal and the effect on fecal FTC are obviously weakened, which shows that there is a certain synergistic effect between the cooked cereal powder and the trachinotus ovatus hypolipidemic peptide. Meanwhile, the content of the cured cereal powder and the lipid-lowering peptide of the trachinotus ovatus have certain upper limits, and as shown in experimental data of examples 5 (excessive cured cereal powder), 7 (excessive cured trachinotus ovatus lipid-lowering peptide) and 9 (excessive cured cereal powder and oval pominotus lipid-lowering peptide), the serum TC level of the rats in the groups is not as reduced as that of examples 2 and 3, and the up-regulation effect on HDL-c in serum and FTC in feces is not as obvious as that of examples 2 and 3. While example 10 using non-ultrafiltrated Trachinotus ovatus peptide powder did not regulate TC, TG, LDL-c, HDL-c and FTC as well as the same amount of Trachinotus ovatus peptide as example 2.
TABLE 2 Effect of intragastric samples on serum blood lipids and fecal blood lipid levels in rats
Note: when the letters marked beside the data are completely different, the difference between groups has statistical significance (p<0.05）。
Claims (4)
1. The nutritional composition for reducing blood fat is characterized by comprising the following components in percentage by mass:
the preparation method of the trachinotus ovatus peptide powder comprises the following steps:
s1, adding 1 time of water into trachinotus ovatus meat and smashing;
s2, adding 2000-6000U/g protease into the crushed trachinotus ovatus in the S1, hydrolyzing for 2-6 hours, inactivating enzyme at 95 ℃, cooling to 4-10 ℃, and centrifuging;
s3, filtering the supernatant obtained after centrifugation in the S2 through a 10,000-3,000 MWCO ultrafiltration membrane, and drying the filtrate to obtain trachinotus ovatus peptide powder;
the content of the cured grain powder in the cured grain powder and the maltodextrin is 60-91%, and the content of the trachinotus ovatus peptide powder in the trachinotus ovatus peptide powder and the skimmed milk powder is 40-80%;
the protein hydrolysate of the trachinotus ovatus peptide powder contains more than 95% of components with relative molecular weight less than 3000 Da.
2. The hypolipidemic nutritional composition of claim 1, wherein the cooked cereal flour is comprised of one or more of cooked oat flour, cooked corn flour, cooked rice flour, cooked black rice flour.
3. The nutritional composition for reducing blood lipid according to claim 1, wherein in S2, the protease is one or more of pepsin, trypsin, alkaline protease and papain; separation factor F in the centrifuger≥800。
4. Use of the hypolipidemic nutritional composition of any one of claims 1 to 3 for the preparation of hypolipidemic drugs, health foods, formulated foods for special medical use or general foods.
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| CN102077945A (en) * | 2010-09-20 | 2011-06-01 | 王强 | Special dietary seafood for postabortal and postpartum women |
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| CN104872677A (en) * | 2015-06-18 | 2015-09-02 | 马慧军 | Dietary composition for benefiting planting and multiplication of probiotics in host body and application thereof |
| CN105146289A (en) * | 2015-08-21 | 2015-12-16 | 刘涛 | Preparation method of health-care product assisting in lowering blood lipids |
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| CN1589670A (en) * | 2003-08-26 | 2005-03-09 | 中国科学院南海海洋研究所 | Body weight reducing health care product extrated from seaweed and its preparation method |
| CN102077945A (en) * | 2010-09-20 | 2011-06-01 | 王强 | Special dietary seafood for postabortal and postpartum women |
| CN102224899A (en) * | 2011-05-25 | 2011-10-26 | 广州邦世迪生物技术有限公司 | Sugar-free homogenate diet |
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