CN106173549A - One carp feed in spring - Google Patents

One carp feed in spring Download PDF

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Publication number
CN106173549A
CN106173549A CN201610626288.2A CN201610626288A CN106173549A CN 106173549 A CN106173549 A CN 106173549A CN 201610626288 A CN201610626288 A CN 201610626288A CN 106173549 A CN106173549 A CN 106173549A
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weight portion
herbal medicine
chinese herbal
weight
medicine additive
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CN201610626288.2A
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唐婷婷
李鑫
黄河
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Shandong New Hope Liuhe Group Co Ltd
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Shandong New Hope Liuhe Group Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses one carp feed in spring, wherein, including following raw material: soybean cake 6 10 weight portion, cottonseed cake 5 10 weight portion, fish flour 0.5 1 weight portion, Fructus Hordei Vulgaris powder 8 10 weight portion, Semen Maydis powder 35 weight portion and Chinese herbal medicine additive 13 weight portion.The invention has the beneficial effects as follows: the feedstuff of the present invention, for the cultivation of Cyprinus carpio in spring, on the one hand decrease the interpolation of traditional raw material, save cost of material, reduce and feed cost;On the other hand, add Chinese herbal medicine additive, improve the physiological function of Cyprinus carpio in spring, reduce prevalence in spring, keep preferably growing sign, lay the foundation for quickly increasing of next season;Furthermore, to be arranged in pairs or groups by science, i.e. reduce feedstuff usage amount, also improve efficiency of feed utilization, feed coefficient improves further, brings the biggest economic benefit for raiser;Finally, the fresh keeping time of feedstuff is longer, is unlikely to deteriorate and goes mouldy.

Description

One carp feed in spring
Technical field
The present invention relates to aquatic feeds, particularly to one carp feed in spring.
Background technology
Along with temperature gos up, Fish just start to ingest, grow, and doing a good job of it fishpond and managing work spring is that to capture High Annual Yield high The important measures of effect.Additionally water temperature in spring bottom out, various can make fish body cause a disease antibacterials, parasite start to multiply in a large number, Meanwhile, Fish are through Wintering Period, and body constitution is more weak, relatively poor to virulence factor resistance, easily infect various diseases, cause pond Fish mortality, the loss caused to culturist is relatively big, and has a strong impact on the growth in living individuals later stage.
Spring, water temperature was relatively low, and fish growth is slow, poor to the absorbance of feed nutrition, therefore, can not seek feedstuff Nutrition is too high, causes waste;Entering summer, water temperature raises very fast, and Fish enter the growth busy season, it should when making full use of this Phase, reach Fish fast-growth;Entering autumn, water temperature begins to decline, and Fish enter and fatten the health care stage, this in period Fish base Originally growing up to, it is important to fatten Fish, keep healthy, therefore, the feeding manner of science should be according to feeding targetedly season, Thus reach because the current situation is suitable.
Further, since the cost of some feedstuffs such as fish flour, soybean cake is rising steadily, price constantly rises, for cultivation Family feed the pressure bringing cost, on the one hand raiser's cost to be spent, improve cultivation efficiency, on the other hand raiser wants Restraining feeds cost, and therefore, how to throttle feeds, and is a big problem of raiser.
How the feedstuff that the spring of Cyprinus carpio is special is provided, meets the various needs of the Cyprinus carpio in spring, reduce Seasonal diseases, And preferably control feeding cost, be the problem to be solved in the present invention.
Summary of the invention
In order to solve the problems referred to above, the invention provides one carp feed in spring.
In order to achieve the above object, the invention provides one carp feed in spring, wherein, including following raw material: bean Cake 6-10 weight portion, cottonseed cake 5-10 weight portion, fish flour 0.5-1 weight portion, Fructus Hordei Vulgaris powder 8-10 weight portion, Semen Maydis powder 3-5 weight Part and Chinese herbal medicine additive 1-3 weight portion.
Wherein, including the raw material of following parts by weight: soybean cake 8 weight portion, cottonseed cake 7 weight portion, fish flour 0.7 weight portion, Fructus Hordei Vulgaris powder 9 weight portion, Semen Maydis powder 4 weight portion and Chinese herbal medicine additive 2 weight portion.
Wherein, described Chinese herbal medicine additive is prepared by the raw material of following parts by weight: Flos Caryophylli 5-10 weight portion, Fructus Litseae 3- 5 weight portions, Herba Portulacae 4-6 weight portion, Fructus Gardeniae 7-12 weight portion, Radix Isatidis 10-15 weight portion, Coriolous Dersicolor (Fr.) Quel 4-9 weight portion, Radix Angelicae Sinensis 4- 8 weight portions, Herba Taraxaci 5-8 weight portion, Flos Lonicerae 10-15 weight portion, Herba Galii Teneri 4-10 weight portion, Radix rubi coreani 3-6 weight portion, old Monarch must 4-7 weight portion, Fructus Rubi 3-5 weight portion, Radix Glycyrrhizae 7-12 weight portion, Semen Sesami Nigrum 4-8 weight portion, Fructus Hordei Germinatus 5-9 weight portion, Huang Even 4-6 weight portion, Herba Bidentis Bipinnatae 6-10 weight portion, Rhizoma Belamcandae 5-8 weight portion, Radix Scutellariae 3-5 weight portion and Herba Lysimachiae 5-10 weight portion.
Wherein, described Chinese herbal medicine additive is prepared by the raw material of following parts by weight: Flos Caryophylli 8 weight portion, Fructus Litseae 4 weight Part, Herba Portulacae 5 weight portion, Fructus Gardeniae 8 weight portion, Radix Isatidis 12 weight portion, Coriolous Dersicolor (Fr.) Quel 6 weight portion, Radix Angelicae Sinensis 6 weight portion, Herba Taraxaci 7 weight Amount part, Flos Lonicerae 12 weight portion, Herba Galii Teneri 6 weight portion, Radix rubi coreani 5 weight portion, Radix Tylophorae arenicolae 5 weight portion, Fructus Rubi 4 weight portion, Radix Glycyrrhizae 8 weight portion, Semen Sesami Nigrum 6 weight portion, Fructus Hordei Germinatus 7 weight portion, Rhizoma Coptidis 5 weight portion, Herba Bidentis Bipinnatae 7 weight portion, Rhizoma Belamcandae 6 weight portion, Radix Scutellariae 4 weight portion and Herba Lysimachiae 7 weight portion.
Wherein, its preparation method is:
Step one: mixed in described ratio by each raw material of described Chinese herbal medicine additive, adds relative to mixture 2~3 times The ethanol that determining alcohol is 80%~90%, be heated to boiling reflux 2~3 hours, filter, use percolation with per minute 1~ The speed of 2ml slowly percolation, collects percolate, when being evaporated to 50~60 DEG C under vacuum 0.05~0.07Mpa subsequently Relative density is the mastic of 1.04~1.10, is spray-dried, the inlet temperature 160 of spray dryer~175 DEG C, leaving air temp 80 ~85 DEG C, it is ground into powder subsequently, makes Chinese herbal medicine additive;
Step 2: by raw material and the Chinese herbal medicine additive mix homogeneously of residual feed, and use pulverizer to carry out further Dispersion and fining, obtains the feedstuff that particle diameter is 200-250um.
In raw material, soybean cake is the side-product after Semen Glycines and Semen sojae atricolor oil expression, and in the dry of every kilogram of soybean cake, digestible energy exists More than 3000 kilocalories, gross protein value, more than 40%, is prepared it before feedstuff under the conditions of 100~110 DEG C, is heated 40 points About clock.
Cottonseed cake is the by-product after Semen Gossypii oil expression, and in cottonseed cake, arginine content is up to 3.6%-3.8%, by cottonseed cake Add the 0.1% of 2 times amount~the ferrous sulfate aqueous solution of 0.2% in soak, mix homogeneously, stirring several times, soak 12-24 hour After, pull out dry, be then used for preparing feedstuff.
Fish flour is to be raw material with one or more Fish, and the high-protein feed through deoiling, being dehydrated, after grinding and processing is former Material, crude fat content is high, rich in vitamin B group, calcium, phosphorus content the highest, selenium content is the highest, iodine, zinc, ferrum, selenium content also The highest, and containing appropriate arsenic possibly together with the somatotrophic unknown factor, can stimulating animal growth promoter.
Fructus Hordei Vulgaris powder: Fructus Hordei Vulgaris nutritional labeling relatively horn of plenty, every 100 grams of moisture contents 13.1 grams, 10.2 grams of protein, fat 1.4 Gram, carbohydrate 63.4 grams, dietary fiber 9.9 grams, calcium 66 milligrams, 381 milligrams of phosphorus, ferrum 6.4 milligrams.Additionally, possibly together with dimension Raw element, thiamine, riboflavin, nicotinic acid, allantoin etc..
Semen Maydis powder: Semen Maydis contains abundant nutrient, containing substantial amounts of lecithin, linoleic acid, grain alcohol, dimension life in Semen Maydis Element E, cellulose etc., have the effects such as lowering blood pressure and blood fat, arteriosclerosis, prevention intestinal cancer, looks improving and the skin nourishing, slow down aging.
Chinese herbal medicine additive is as a kind of biotype additive, it is possible to well meet while feeding nutrition, it is ensured that raise Feed trophism, it is possible to reduce fish flour, soybean cake and the addition of some traditional raw materials, cost-effective.Wherein, Flos Caryophylli is Fructus Rhodomyrti The dry flower of section's plant of clove.Pluck when alabastrum is turned red by green, dry.Alabastrum contains volatile oil, containing eugenol, second in oil Acyl eugenol and a small amount of Flos Caryophylli alkene, MAK, the positive heptanone of methyl, vanillin;Additionally, still containing oleanolic acid, tannin, Fatty oil and cured.Fruit contains volatile oil, has preferable bacteriostasis antibiosis anthelmintic action.
Fructus Litseae is Lauraceae Litsea plant cubeb litsen tree, with fruit, root and leaf.Gather during fruit maturation in autumn, root, leaf The whole year can adopt, and removes impurity, dries.Fruit contains volatile oil, is wherein mainly composed of citral, and has limonene, sabinene, methyl Heptanone, citronellal etc., have preferable bacteriostasis.
Herba Portulacae is portulacaceous plant Herba Portulacae, with all herbal medicine.Herb contains levarterenol, and containing many Ba Ming and a small amount of DOPA;Additionally, still the sample material Han vitamin A, vitamin B1, B2, PP, C, carotene, Saponin, tannin, tree Fat, fat, carbamide, calcium, phosphorus, iron salt, potassium binoxalate, potassium chloride, potassium nitrate, potassium sulfate and other potassium salt.Possibly together with abundant The oxalic acid of malic acid, citric acid, aminoacid, oxalates and micro free.Herb aobvious alkaloid, coumarin, flavone, cardiotonic glycoside And anthraquinone analog compound reaction.Containing abundant vitamin A sample material, the physiological function of epithelial cell can be promoted, to large intestine bar Bacterium, dysentery bacterium, Bacillus typhi all have inhibitory action;Common Pathogenic dermatophytes also there is is inhibitory action, blood vessel is had aobvious Write ground contraction.
Fructus Gardeniae is the dry mature fruit of Maguireothamnus speciosus Fructus Gardeniae, and its root also can be used as medicine.9~fruit maturation in November be reddish yellow Gather during color, remove carpopodium and impurity, steam supreme vapour or put in boiling water boiling the hottest, take out, be dried.Gen Xiaqiu excavates, and cleans and dries. Containing flavonoid gardenin, pectin, tannin, crocin, crocetin, PEARLITOL 25C, nonacosane, cupreol.Another containing multiple The glycoside of tool ring smelly formaldehyde structure: gardenoside, geniposide pool element-1-glycoside, lattice Tegosept P-1-β-D-gentiobioside And shanzhiside in a small amount, there is function of gallbladder promoting anti-microbial effect.
The root of Radix Isatidis is containing indigo glycoside, cupreol, isatin, Radix Isatidis crystallization second, Radix Isatidis crystallization third, Radix Isatidis crystallization Fourth.Again containing vegetable protein, resin-like thing, saccharide etc..In root aminoacid have arginine, proline, glutamic acid, tyrosine, γ- Aminobutyric acid, valine and leucine.Again containing sinigrin.Possibly together with resisting gram-positive and the antibacterial substance of negative bacteria and dynamic Power essence, has antibiosis and antiviral functions.
Coriolous Dersicolor (Fr.) Quel is the sporophore of On Polyporaceae Corilus versicolor Quel..The mycelia of Corilus versicolor Quel. culture is got sugar egg In vain, there is raising immunity, antibacterial antifungal effect.
Angelica root contains volatile oil, and volatilization main body of oil has butylidenephthalide, the adjacent positive pentanone of carboxyl benzene and Δ 2,4-bis- Hydrogen acid phthalic anhydride, has raising immunization.
Herba Taraxaci is feverfew Herba Taraxaci, alkali ground Herba Taraxaci or the dry herb belonging to several plants together.Spring to autumn is spent Excavate when just opening, remove impurity, clean, dry.Herba Taraxaci herb, containing taraxasterol, choline, inulin and pectin etc., has relatively Strong sterilizations etc. act on.
Flos Lonicerae is caprifoliaceae plant Radix Ophiopogonis, Flos Lonicerae, Flos Lonicerae or the dry flower of hair style Radix Ophiopogonis or carries first opening Flower.Early summer the flowers are in blossom put before gather, be dried.Flower, containing luteolin, inositol and Saponin, tannin etc., has preferable antibacterial action.
Herba Galii Teneri is that Rubiaceae clivers Herba Galii Teneri is with all herbal medicine.Summer gathers, using fresh herb or dry.Nature and flavor Pungent, bitter, cool, cure mainly heat-clearing and toxic substances removing, inducing diuresis to remove edema.
Radix rubi coreani is the root of rosaceous plant rubus coreanus.Nature and flavor are pained;Flat;Nontoxic.Return through liver;Kidney channel.Promoting blood circulation and hemostasis, dispels Wind dehumidifies.Spit blood, epistaxis, rheumatic arthralgia, edema.
Radix Tylophorae arenicolae is the root and rhizome of peento in trailing plants section plant snow.Summer, autumn excavate, and remove aerial parts, dry.Cure mainly The kidney invigorating, spleen invigorating, removing toxic substances.Chronic consumptive disease chronic cough, edema.
Fructus Rubi is the dry fruit of Rosaceae rubus rubus chingii Hu.Early summer fruit by green change greenish-yellow time adopt Receive, remove stalk, leaf, put and boiling water slightly scalds or slightly steams, take out, be dried.Return kidney, bladder warp.Kidney tonifying, controlling nocturnal emission with astringent drugs, reducing urination.
Radix Glycyrrhizae is the dry root of glycyrrhizic legume, Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L..Spring, season in autumn two excavate, and remove fibrous root, Dry.Nature and flavor are sweet, flat.GUIXIN, lung, spleen, stomach warp.Invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.Empty for taste Weak, fatigue and weakness, shortness of breath and palpitation, gastral cavity abdomen, extremity contraction urgency pain, carbuncle sore tumefacting virus, cushion toxicity, strong.
Semen Sesami Nigrum is the dry mature seed of Pedaliaceae (Pedaliaceae) Sesamum plant sesame.Tap during fruit maturation in autumn Plant, dries, and lays seed, removes impurity, then dries.Nature and flavor are sweet, flat.Return liver, kidney, large intestine channel.Invigorating the liver and kidney, benefiting essence-blood, profit Dryness of the intestine.
Fructus Hordei Germinatus is that the mature fruit of grass Fructus Hordei Vulgaris is through dry obtaining of germinateing.After being soaked in water by wheat grain, keep suitable Preferably epidemic disaster, when plumelet length to about 0.5cm, dries or cold drying.Nature and flavor are sweet, flat.Return spleen, stomach warp.Circulation of qi promoting helps digestion, strong Spleen whets the appetite, relieving distension.Do not disappear for food stagnation, abdominal distention, insufficiency of the spleen lack of appetite.
Rhizoma Coptidis is the dry rhizome of ranunculaceae plant Rhizoma Coptidis, Coptis deltoidea C.Y.Cheng et Hsiao or Coptis Teeta Wall.Autumn excavates, and removes fibrous root and mud Sand, is dried, and hits residual fibrous root.Rhizoma Coptidis contains berberine, coptisine, and 13-methyl-.psi.-coptisine., the palm leaf coculine, Columbamine etc. are raw Alkaloids, still containing obacunone, obakulactone, has antimicrobial protozoacide effect,
Herba Bidentis Bipinnatae is the herb of feverfew bidens pilosa.Sweet in the mouth;Micro-hardship;Cool in nature.Heat clearing away;Removing toxic substances;Dampness removing;Spleen invigorating. Main enteritis;Dysentery;Acute appendicitis;Blood deficiency general edema with yellowish skin;There is antimicrobial protozoacide effect.
Rhizoma Belamcandae is the dry rhizome of irides Rhizoma Belamcandae.Just germinate at the beginning of spring or autumn end stem and leaf has been excavated when withering, removed palpus Root and silt, be dried.Rhizome contains belamcandin, tectoridin, shekanin, tectorigenin.Flower, leaf, containing Mengiferin, have anti-micro- Biological agent.
Radix Scutellariae is the dry root of labiate Radix Scutellariae.Spring, season in autumn two excavate, and remove fibrous root and silt, hit thick after solarization Skin, dries.Scutellariae,radix is containing baicaligenin, baicalin, wogonin, wogonoside and neobaicalein, also containing benzoic acid, β-paddy steroid Alcohol etc..Containing scutellarin in stem and leaf, there is antiinflammatory anti-microbial effect.
Herba Lysimachiae is the dry herb of Ofthe Primulaceae Lysimachia christinae Hance.Summer, season in autumn two gather, and remove impurity, dry.Money Grass has aroma type and non-aromatic odor type two class.Aroma type monoterpene ketone Han volume, its main component is l-pinocamphone, l-menthone and l- Pulegone;Still containing australene, nopinene, limonene, cymol, isomenthone, isopinocamphone, linalool, Herba Menthae Alcohol, alpha-terpineol.In addition to above-mentioned volatile oil component, still containing ursolic acid, cupreol, Palmic acid, succinic acid, several amino acids, Tannin, amaroid, choline, potassium nitrate etc..Under ground portion contains stachyose, has antiinflammatory and improves immunization.
The invention has the beneficial effects as follows: the feedstuff of the present invention, for the cultivation of Cyprinus carpio in spring, on the one hand decrease tradition former The interpolation of material, saves cost of material, reduces and feed cost;On the other hand, add Chinese herbal medicine additive, improve Cyprinus carpio in spring The physiological function of fish, reduces prevalence in spring, keeps preferably growing sign, establishes base for quickly increasing of next season Plinth;Furthermore, to be arranged in pairs or groups by science, i.e. reduce feedstuff usage amount, also improve efficiency of feed utilization, feed coefficient carries further Height, brings the biggest economic benefit for raiser;Finally, the fresh keeping time of feedstuff is longer, is unlikely to deteriorate and goes mouldy.
Detailed description of the invention
It is further illustrated by detailed description below.
Embodiment 1 Chinese herbal medicine additive I
Composition: Flos Caryophylli 50g, Fructus Litseae 30g, Herba Portulacae 40g, Fructus Gardeniae 70g, Radix Isatidis 100g, Coriolous Dersicolor (Fr.) Quel 40g, Radix Angelicae Sinensis 40g, Herba Taraxaci 50g, Flos Lonicerae 100g, Herba Galii Teneri 40g, Radix rubi coreani 30g, Radix Tylophorae arenicolae 40g, Fructus Rubi 30g, Radix Glycyrrhizae 70g, Semen Sesami Nigrum 40g, Fructus Hordei Germinatus 50g, Rhizoma Coptidis 40g, Herba Bidentis Bipinnatae 60g, Rhizoma Belamcandae 50g, Radix Scutellariae 30g and Herba Lysimachiae 50g.
Preparation method: being mixed by each raw material of Chinese herbal medicine additive, adding relative to the determining alcohol of 2 times of mixture is 80% Ethanol, is heated to boiling reflux 2 hours, filters, and uses percolation with the speed slowly percolation of 1ml per minute, collects percolate, Being evaporated to relative density when 50 DEG C subsequently under vacuum 0.06Mpa is the mastic of 1.04, is spray-dried, spray dryer Inlet temperature 160 DEG C, leaving air temp 80 DEG C, be ground into powder subsequently, make Chinese herbal medicine additive I.
Embodiment 2 Chinese herbal medicine additive II
Composition: Flos Caryophylli 100g, Fructus Litseae 50g, Herba Portulacae 60g, Fructus Gardeniae 120g, Radix Isatidis 150g, Coriolous Dersicolor (Fr.) Quel 90g, Radix Angelicae Sinensis 80g, Herba Taraxaci 80g, Flos Lonicerae 150g, Herba Galii Teneri 100g, Radix rubi coreani 60g, Radix Tylophorae arenicolae 70g, Fructus Rubi 50g, Radix Glycyrrhizae 120g, black Semen Sesami 80g, Fructus Hordei Germinatus 90g, Rhizoma Coptidis 60g, Herba Bidentis Bipinnatae 100g, Rhizoma Belamcandae 80g, Radix Scutellariae 50g and Herba Lysimachiae 100g.
Preparation method: each raw material of described Chinese herbal medicine additive mixed, adds and relative to the determining alcohol of 3 times of mixture is The ethanol of 90%, is heated to boiling reflux 2 hours, filters, and uses percolation with the speed slowly percolation of 1ml per minute, collects Percolate, being evaporated to relative density when 60 DEG C subsequently under vacuum 0.07Mpa is the mastic of 1.10, is spray-dried, spray The inlet temperature of mist drying machine 175 DEG C, leaving air temp 85 DEG C, be ground into powder subsequently, make Chinese herbal medicine additive II.
Embodiment 3 Chinese herbal medicine additive III
Composition: Flos Caryophylli 80g, Fructus Litseae 40g, Herba Portulacae 50g, Fructus Gardeniae 80g, Radix Isatidis 120g, Coriolous Dersicolor (Fr.) Quel 60g, Radix Angelicae Sinensis 60g, Herba Taraxaci 70g, Flos Lonicerae 120g, Herba Galii Teneri 60g, Radix rubi coreani 50g, Radix Tylophorae arenicolae 50g, Fructus Rubi 40g, Radix Glycyrrhizae 80g, Semen Sesami Nigrum 60g, Fructus Hordei Germinatus 70g, Rhizoma Coptidis 50g, Herba Bidentis Bipinnatae 70g, Rhizoma Belamcandae 60g, Radix Scutellariae 40g and Herba Lysimachiae 70g.
Preparation method: each raw material of described Chinese herbal medicine additive mixed, adds and relative to the determining alcohol of 2 times of mixture is The ethanol of 80%, is heated to boiling reflux 2 hours, filters, and uses percolation with the speed slowly percolation of 2ml per minute, collects Percolate, being evaporated to relative density when 54 DEG C subsequently under vacuum 0.06Mpa is the mastic of 1.06, is spray-dried, spray The inlet temperature of mist drying machine 165 DEG C, leaving air temp 85 DEG C, be ground into powder subsequently, make Chinese herbal medicine additive III.
The safety testing of Chinese herbal medicine additive
One, acute toxicity test
1 test method
1.1 animal subjects: cleaning grade ICR mice, about 20 grams every, male and female half and half.
1.2 test medicine: the Chinese herbal medicine additive III of the embodiment of the present invention 3.
The half maximum tolerance determination of the Chinese herbal medicine additive III of 1.3 embodiment of the present invention III: 40 mices are pressed Body weight equilibrium is divided into two groups, often group 20, male and female half and half, sub-cage rearing.Experiment prospective adaptation is observed 1 week, after fasting 16 hours, Concentration of ordinary dissolution (1.6g mL with Chinese herbal medicine additive III-1) gavage, once, single oral gavage capacity is every to every 4 hours gavages 0.5mL, gavages three times continuously, and gavaging total capacity is every 1.5mL, and always gavaging dosage is 120g kg body weight-1, matched group gavages Equivalent distilled water.Observe the instant of animal after being administered and toxicity, death condition in 14 days.Observe mice behavioral activity, The mental status, hair color gloss, drinking-water of ingesting, Excreta situation, nose, eye, oral cavity with or without situations such as abnormal secretion thing and death, Continuous Observation 14 days.Put to death all mices in the 14th day, observe the pathological change of the internal organs such as the heart, liver, spleen, lung, kidney, brain and take group Knit rapidly as fixing in the neutral Liquor Formaldehyde of 4%, be used for making histopathologic slide and carry out the inspection of conventional H .E..Record is given Before medicine and be administered after the 7th day and the body weight of the 14th day mice.
1.4 Histomorphologicals and analysis
Preliminary observation organizational structure under an optical microscope, is analyzed.Contrast test group and the organizational structure of matched group, Find liver and the damage of renal tissue of test group mice organs tissue, particularly metabolism burden weight.
1.5 data statisticss and analysis
Body weight unit is gram (g), and experimental result data is expressed as mean+SD, uses SPSS (17.0) to add up soft Comparing between organizing between the student t check groups in part, P < 0.05 is i.e. judged to that significant difference, P < 0.01 are i.e. judged to difference The most notable.
2 results and analysis
2.1 median lethal dose(LD 50) measurement results
Test group mouse stomach be administered after, drinking-water, skin, by hair, spirit, behavior, mouth and nose discharge of eye, Excreta (excrement Just) etc. all right, searches for food and autonomic activities has increased slightly, and remaining project and control group mice are without being clearly distinguished from.Within 3rd day, adopt Appetite and autonomic activities return and drop to be administered front level, without being clearly distinguished from compared with control group mice.Without little in 7 days of observation period Mus is dead, therefore Chinese herbal medicine additive III does not exist the median lethal dose(LD 50) to mice and minimum lethal dose, therefore measures mice to medium-height grass The maximum tolerated dose of medicine additive III.
2.2 maximum tolerance determination results
After test group mouse stomach is administered, do not occur closing one's eyes, sleeping volt, the bad phenomenon such as restless.
The maximum tolerated dose multiple of mice is calculated as follows:
Mus maximum tolerated dose every day: 1.6g ml × 0.56m 0.02kg body weight-1× 3 skies-1=120g kg body Weight-1
The current status of 2.3 experimental periods
After test group mouse stomach is administered, do not occur closing one's eyes, crouching the bad phenomenon such as volt.Compared with matched group, test group is little Mus autonomic activities increases, and searches for food and also increased, and feces is pitchy.Within the observation period, test group mice is abnormal without cry, shake Quiver, faint from fear, movement disorder, sialorrhea, shed tears, rhinorrhea, dyspnea, heart beating exception, diarrhoea, constipation, the phenomenon such as intestinal tympanites are sent out Raw, mice fur gloss, in being administered latter 14 days, there is not death in mice.Test group mice searches for food, drinking water all belongs to normal.Additionally, Test group mouse skin, the best by hair, mental status, behavior, eyelid, eyeball, breathing, mouth and nose discharge of eye, Excreta situation Good, with matched group without being clearly distinguished from.
2.4 body weight change
Mice before being given Cmax and extremely large amount of Chinese herbal medicine additive III and be administered after 7 days, 14 days, body Trace difference is had on heavily.Concrete condition see table 1.
Table 1 medicine I is on the impact (n=20) of Mouse Weight (unit: gram)
Group 0th day 7th day 14th day
Matched group 20.45±1.65 25.34±2.56 30.34±2.09
Test group 20.35±1.71 29.32±2.67 35.25±2.14
As shown in Table 1, after heavy dose of administration, Chinese herbal medicine additive III has facilitation to the body weight of mice.
2.5 system postmortems and histopathologic examination's result
The anatomic observation heart, liver, spleen, lung, kidney, brain are all without the most substantially pathological changes, and histopathologic examination also has no the most different Often.
This result of the test shows, ICR mice gavages medicine I aqueous solution for 3 times and reaches 120g kg body weight in 12 hours-1(quite In human body recommended dose 0.5g kg-1240 times) time without obvious toxicity, Chinese herbal medicine additive III is peroral administration in prompting Safe-dosaging limits is wider.It is generally believed that according to the weight, 1 day maximum dosage-feeding of mice is equivalent to clinical consumption per day of being grown up More than 100 times is exactly safer.The above results shows, Chinese herbal medicine additive III gavages mice with maximum dosage-feeding and do not produces Raw acute toxicity.
Two, subacute toxicity test
2.1 test method
2.1.1 animal subject: SD rat, about 200 grams every, male and female half and half.
2.1.2 test medicine: the Chinese herbal medicine additive II of the embodiment of the present invention 2
2.1.3 Chinese herbal medicine additive II subchronic toxicity test: by 80 healthy cleaning grade SD rats at isothermal experiments Room (temperature: 24 ± 1 DEG C, humidity: 50 ± 10%) adapts to 7 days.Male and female half and half, are randomly divided into 4 groups by body weight equilibrium, i.e. Chinese herbal medicine Additive II high, medium and low dosage group and matched group, often group 20, rat freely drinks water.High dose group gives Chinese herbal medicine additive II 100g kg body weight-1(be equivalent to effective dose 0.5g kg body weight of being grown up-1200 times), middle dosage group gives Chinese herbal medicine Additive II 50g kg body weight-1(be equivalent to effective dose 0.5g kg body weight of being grown up-1100 times), during low dose group gives Chinese herbal medicine additive agent II 10g kg-1 (is equivalent to effective dose 0.5g kg body weight of being grown up-120 times), matched group give wait hold Long-pending distilled water.Being administered volume is 0.75mL 100g-1Body weight, once a day, successive administration 7 days, change every day bedding and padding, drinking-water with And diet, weigh every day once, adjust dosage according to body weight change.
Continue after drug withdrawal to observe 28 days, record weekly feed intake and body weight.Fasting 12 hours after off-test, disconnected neck collection Blood, closes in heparin sodium anticoagulant blood-collecting pipe, carries out hematological examination.The blood collected is used 1500rpm, within 10 minutes, divides Blood biochemical analysis inspection is carried out from serum.After obduction, the main organs such as the systems inspection heart, liver, spleen, lung, kidney, gathers afterwards The tissue of the internal organs such as the heart, liver, spleen, lung, kidney makes pathological section, carries out pathological examination after using H.E. dyeing.
2.1.4 hematology's blood routine examination
Carry out hematology's routine blood indexes inspection, including hemoglobin (HGB), erythrocyte (RBC), MCV (MCV), average hemoglobin content (MCH), packed cell volume (HCT), average hemoglobin content (MCH), erythrocyte are average Hemoglobin concentration (MCHC), Red cell distribution width (RDW), reticulocyte (RETIC), total white blood cells (WBC), Neutrophilic granulocyte (NEU), lymphocyte (LYM), mononuclear cell (MONO), eosinophilic granulocyte (EOS), basophilic granulocyte (BASO), the inspection of 16 projects such as mean platelet volume (MPV) and MPW (PDW).
2.1.5 blood biochemical analysis inspection
Blood biochemical analysis index checking, including glutamate pyruvate transaminase (ALT), glutamic oxaloacetic transaminase, GOT (AST), blood urea nitrogen (BUN) with And the inspection of the index such as creatinine (CREA).
2.1.6 Histomorphological and analysis
Preliminary observation organizational structure under an optical microscope, is analyzed.Contrast test group and the organizational structure of matched group, Find liver and the damage of renal tissue of test group mice organs tissue, particularly metabolism burden weight.
2.1.7 data statistics and analysis
Body weight unit is gram (g), and experimental result data is expressed as mean+SD, uses SPSS (20.0) to add up soft One factor analysis of variance (One-Way ANOVA) model in part carries out group difference significance test, and P < 0.05 is i.e. judged to Significant difference, P < 0.01 is i.e. judged to that difference is the most notable.
2.2 result and analysis
2.2.1 the current status of experimental period
Rats in test groups is after gastric infusion, and do not occur closing one's eyes, crouching the bad phenomenon such as volt.Within 7 days administration phases, with right Compare according to group, rats in test groups does not all occur that cry is abnormal, trembles, faints from fear, movement disorder, sialorrhea, shed tears, rhinorrhea, breathing The phenomenon such as difficulty, heart beating exception, diarrhoea, constipation, intestinal tympanites, rat fur light is soft and smooth, in 7 days of administration and the observation period In 28 days, all there is not the death caused because of drug toxicity in rat.Administration group rat searches for food, drinking water all belongs to normal.Additionally, be administered Group rat within 28 day observation period, skin, by hair, mental status, behavior, eyelid, eyeball, breathing, mouth and nose discharge of eye, excretion Principle condition is the most normal, all without being clearly distinguished from compared with matched group.
2.2.2 body weight change
In experimental period, each administration group rat (female, male) body weight with matched group than more significant no difference of science of statistics.Namely Say, 7 days take medicine, 28 days conventional raise after, be administered between each group, same sex rat body weight and there was no significant difference (P > 0.05).Concrete outcome see table.(table 2)
The impact (n=10) on rat body weight female, male of the table 2 medicine II gavage
As shown in Table 2, same sex rat, within experimental period, does not all have significant difference in body weight between each group.Show long-term After taking Chinese herbal medicine additive II, along with the increase of dosage, the body weight of rat increases slightly fast, shows that this Chinese herbal medicine additive is to greatly Mus body weight has increase facilitation.
2.2.3 hematology's blood routine examination result
Hematology results shows, the Chinese herbal medicine additive II gavage hematological indices every to rat of various dose is equal Do not make significant difference.It is to say, 7 days take medicine, 28 days conventional raise after, every hematological indices of each group rat there is no Significant difference (P > 0.05).Concrete outcome see table.(table 3)
As shown in Table 3, rat, within experimental period, does not has particularly significant property poor in every blood routine project between each group Different.This result shows, after long-term taking Chinese herbal medicine additive II, rat will be caused obvious hematology to affect by medicine.
2.4.4 blood biochemical analysis checks that result blood biochemical analysis testing result shows, various dose Chinese herbal medicine additive II Solution gavage is to all do not make significant difference (P > 0.05) such as glutamate pyruvate transaminase, glutamic oxaloacetic transaminase, GOT and the creatinines of rat.Dosage high, middle Chinese herbal medicine additive II gavage urea nitrogen content relatively control rats in rat blood can be caused to significantly reduce (P < 0.05) (table 4), but still in normal physiologic range.
The impact (n=20) on rat blood biochemical indexes of the table 4 Chinese herbal medicine additive II gavage
As shown in Table 4, rat is within experimental period, except all not having in every blood biochemical analysis project of blood urea nitrogen between each group There is significant difference, but the blood urea nitrogen index of each group is all within normal physiologic range.Therefore, after prolonged administration of drugs II, medicine Rat will be caused obvious blood biochemical analysis to affect by thing.
2.4.5 system postmortem and histopathologic examination's result
Postmortem has no and substantially substantially changes, and the internal organs such as the various dose drug treating group rat heart, liver, spleen, lung and kidney are intrinsic Clear in structure, without pathological change.
3 sum up
This result of study shows, within continuous 7 days, gavages various dose Chinese herbal medicine additive II aqueous solution spiritual to rat, OK All not having undesirable effect for, activity, hair color, Excreta etc., drug withdrawal also had no that rat has obvious behavioristics, body surface in 28 days Feature and Excreta are abnormal.
In 28 days of drug withdrawal observation period, weight differences of respectively organizing with sex between rat that there are no significant, hematology and blood are raw Change index also no abnormality seen.Additionally, in blood biochemistry detects, rat urea nitrogen levels, middle and high dosage group does not show with matched group Write sex differernce.Owing to the rat blood urea nitrogen index of gavage middle and high dosage group is still within normal range, in conjunction with routine blood test two-story valley third From the point of view of transaminase's index, the rat gpt level of the Chinese herbal medicine additive II group of gavage high dose, compared with matched group There is no significant difference and within normal range, show maximum concentration of ordinary dissolution Chinese herbal medicine additive II liver is not had poison Side effect.And the urea nitrogen levels of each group rat of gavage Chinese herbal medicine additive II is in various degree less than matched group, in conjunction with kidney From the point of view of dirty structure observation, show that the Chinese herbal medicine additive II of any dosage has certain protective effect to kidney.Pathological tissue Learn testing result and show oral various dose Chinese herbal medicine additive II aqueous solution not to main organs such as the heart, liver, spleen, lung, kidneys not Cause substantially damage.
Therefore, animal will not be caused subchronic injury or subchronic toxicity by Chinese herbal medicine additive II oral administration.
Comprehensively, the Chinese herbal medicine additive that the present invention adds, not there is acute toxicity, the most not there is inferior toxicity and long-term poison Property.
Embodiment 4 feedstuff I
Composition: soybean cake 600g, cottonseed cake 500g, fish flour 50g, Fructus Hordei Vulgaris powder 800g, Semen Maydis powder 300g and Chinese herbal medicine additive 100g;Wherein Chinese herbal medicine additive uses the Chinese herbal medicine additive I that embodiment 1 prepares.Wherein, for the pretreatment of soybean cake It is by it under the conditions of 100 DEG C, heats 40 minutes, dry.Cottonseed cake is added 2 times amount 0.1% ferrous sulfate aqueous solution Middle immersion, mix homogeneously, stirring several times, after soaking 12 hours, is pulled out dry.Various raw materials after above-mentioned process are mixed, and Use pulverizer to carry out further dispersion and fining, obtain the feedstuff I that particle diameter is 200um.
Embodiment 5 Feed II
Composition: soybean cake 1000g, cottonseed cake 1000g, fish flour 100g, Fructus Hordei Vulgaris powder 1000g, Semen Maydis powder 500g and Chinese herbal medicine add Add agent 300g;Wherein Chinese herbal medicine additive uses the Chinese herbal medicine additive II that embodiment 2 prepares.Wherein, for soybean cake Pretreatment is by it under the conditions of 110 DEG C, heats 50 minutes, dries.Cottonseed cake is added 2 times amount 0.2% ferrous sulfate Soaking in aqueous solution, mix homogeneously, stirring several times, after soaking 24 hours, is pulled out dry.Various raw materials after above-mentioned process are mixed Close, and use pulverizer to carry out further dispersion and fining, obtain the Feed II that particle diameter is 250um.
Embodiment 6 Feed II I
Composition: soybean cake 800g, cottonseed cake 700g, fish flour 70g, Fructus Hordei Vulgaris powder 900g, Semen Maydis powder 400g and Chinese herbal medicine additive 200g;Wherein Chinese herbal medicine additive uses the Chinese herbal medicine additive III that embodiment 3 prepares.Wherein, for the pre-place of soybean cake Reason is by it under the conditions of 105 DEG C, heats 45 minutes, dries.Cottonseed cake is added 2 times amount 0.2% ferrous sulfate water-soluble Soaking in liquid, mix homogeneously, stirring several times, after soaking 20 hours, is pulled out dry.Various raw materials after above-mentioned process are mixed, And use pulverizer to carry out further dispersion and fining, obtain Feed II I that particle diameter is 220um.
Test
Test site: 4 test ponds specifications be 10 mus/mouthful, the depth of water about 2.0 meters, all through desilting, shine the pool, Lime disinfection and training water.
Test period: in March, 2015-May.
Subjects: choose carp fry, fingerling, after 2% saline solution sterilization, renders to test on March 1st, 2015 In pond.
The Cyprinus carpio stocking model of each test tank in pond is basically identical, as shown in table 1.
Table 1: the carp culture specification (10 mu/mouthful pond) that test pond is respectively organized
Fingerling Fingerling specification (g/ tail) Fingerling quantity (tail) Fingerling gross weight (kg)
Matched group 20.56 3000 60.68
Feedstuff I group 20.33 3000 60.99
Feed II group 20.35 3000 61.05
Feed II I group 20.42 3000 61.26
Test feed: buy 1t common carp feedstuff;It is equipped with each 1t of feedstuff I-III simultaneously, is placed under normal temperature condition and carries out Deposit.
Wherein, the feedstuff of control group fed is common carp feedstuff, and proportioning is: soybean cake 40%, cottonseed cake 20%, fish flour 10%, Fructus Hordei Vulgaris powder 10%, Semen Maydis powder 17.5%, Sal 0.5% and vitamin mineral additive 2%;
The feedstuff I that feedstuff is embodiment 4 that feedstuff I group feeds;
The feedstuff that Feed II group feeds is the Feed II of embodiment 5;
The feedstuff that Feed II I group feeds is Feed II I of embodiment 6.Table 2 is the Costco Wholesale contrast of each feedstuff
Feedstuff Unit price (unit/kg) Quality (t) Totle drilling cost price (first)
Common carp feedstuff 5.98 1 5980
Feedstuff I 4.26 1 4260
Feed II 4.35 1 4350
Feed II I 4.23 1 4230
Unified management is responsible for by special messenger in all ponds.
Table 3: respectively organize reference table of throwing something and feeding Cyprinus carpio pond normal feedstuff day
Observation item
1, observe the body weight change before and after feeding, calculate feed cost.
2, observe the Cyprinus carpio changes of vital signs before and after feeding, record disease incidence situation.
3, randomly draw sample and carry out blood biochemical detection and internal organs detection.
4, residual feed is carried out detection to go mouldy.
Result of the test
Table 4 is that feed conversion, cost and the residue of each group is gone mouldy contrast
As shown in Table 4, three present invention feed group, scale of feeding and feed cost and be all significantly lower than matched group.And remain Going mouldy does not occurs in remaining feedstuff.
Fishing for 100 tail Cyprinus carpios from each group of pond carries out measured body weight at random.
Table 5 is the contrast situation of each group
Group Original body mass (g) Mantissa's (tail) TBW (kg) Average weight (g/ tail)
Matched group 20.3 100 112.3 1123
Feedstuff I group 20.2 100 145.4 1454
Feed II group 20.3 100 145.8 1458
Feed II I group 20.2 100 146.1 1461
As shown in Table 5, feeding of three feedstuff groups, Cyprinus carpio increases weight apparently higher than matched group.It follows that the present invention's raises Material, for the weightening finish of Cyprinus carpio in spring, has good facilitation.
Table 6 is the dead fish phenomenon during the nursing in each group of pond and ill occurs that situation contrasts
Group Enteritis disease Fish molds The Cyprinus carpio spring is sick Scale protrusion disease Lernaean Dead fish number
Matched group Have Have Have Have Have 200-400
Feedstuff I group Nothing Nothing On a small quantity Nothing Nothing 50-80
Feed II group Nothing On a small quantity Nothing Nothing Nothing 40-80
Feed II I group Nothing Nothing On a small quantity Nothing Nothing 40-80
As shown in Table 6, the Cyprinus carpio in the pond of three feedstuff groups, disease condition is much better than matched group, and dead fish quantity is the most relatively Matched group has greatly improved.
50 Cyprinus carpios of each group pond extraction, dissect, detect for liver pancreas lipid metabolism and Antioxidant Indexes, Result such as table 7
As shown in Table 7, feeding of three feedstuff groups, the resistance to oxidation of Cyprinus carpio and metabolism will be better than matched group.Thus, originally The feedstuff of invention, promotes Cyprinus carpio metabolism, improves the resistance to oxidation of Cyprinus carpio, promotes the vital functions of Cyprinus carpio.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and Within principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.

Claims (5)

1. one kind spring carp feed, it is characterised in that include following raw material: soybean cake 6-10 weight portion, cottonseed cake 5-10 weight Amount part, fish flour 0.5-1 weight portion, Fructus Hordei Vulgaris powder 8-10 weight portion, Semen Maydis powder 3-5 weight portion and Chinese herbal medicine additive 1-3 weight portion.
Spring the most according to claim 1 carp feed, it is characterised in that include the raw material of following parts by weight: bean Cake 8 weight portion, cottonseed cake 7 weight portion, fish flour 0.7 weight portion, Fructus Hordei Vulgaris powder 9 weight portion, Semen Maydis powder 4 weight portion and Chinese herbal medicine add Agent 2 weight portion.
Spring the most according to claim 1 and 2 carp feed, it is characterised in that described Chinese herbal medicine additive is by following The raw material of parts by weight prepares: Flos Caryophylli 5-10 weight portion, Fructus Litseae 3-5 weight portion, Herba Portulacae 4-6 weight portion, Fructus Gardeniae 7-12 weight Amount part, Radix Isatidis 10-15 weight portion, Coriolous Dersicolor (Fr.) Quel 4-9 weight portion, Radix Angelicae Sinensis 4-8 weight portion, Herba Taraxaci 5-8 weight portion, Flos Lonicerae 10- 15 weight portions, Herba Galii Teneri 4-10 weight portion, Radix rubi coreani 3-6 weight portion, Radix Tylophorae arenicolae 4-7 weight portion, Fructus Rubi 3-5 weight portion, sweet Grass 7-12 weight portion, Semen Sesami Nigrum 4-8 weight portion, Fructus Hordei Germinatus 5-9 weight portion, Rhizoma Coptidis 4-6 weight portion, Herba Bidentis Bipinnatae 6-10 weight portion, penetrate Dry 5-8 weight portion, Radix Scutellariae 3-5 weight portion and Herba Lysimachiae 5-10 weight portion.
Spring the most according to claim 3 carp feed, it is characterised in that described Chinese herbal medicine additive is by following weight The raw material of number prepares: Flos Caryophylli 8 weight portion, Fructus Litseae 4 weight portion, Herba Portulacae 5 weight portion, Fructus Gardeniae 8 weight portion, Radix Isatidis 12 weight Amount part, Coriolous Dersicolor (Fr.) Quel 6 weight portion, Radix Angelicae Sinensis 6 weight portion, Herba Taraxaci 7 weight portion, Flos Lonicerae 12 weight portion, Herba Galii Teneri 6 weight portion, life of falling Root 5 weight portion, Radix Tylophorae arenicolae 5 weight portion, Fructus Rubi 4 weight portion, Radix Glycyrrhizae 8 weight portion, Semen Sesami Nigrum 6 weight portion, Fructus Hordei Germinatus 7 weight portion, Rhizoma Coptidis 5 weight portion, Herba Bidentis Bipinnatae 7 weight portion, Rhizoma Belamcandae 6 weight portion, Radix Scutellariae 4 weight portion and Herba Lysimachiae 7 weight portion.
5. according to the carp feed in spring described in any one of claim 1-4, it is characterised in that its preparation method is:
Step one: mixed in described ratio by each raw material of described Chinese herbal medicine additive, adds relative to mixture 2~the alcohol of 3 times Concentration is the ethanol of 80%~90%, is heated to boiling reflux 2~3 hours, filters, and uses percolation with per minute 1~the speed of 2ml Spend slowly percolation, collect percolate, under vacuum 0.05~0.07Mpa, be evaporated to relative density when 50~60 DEG C subsequently It is the mastic of 1.04~1.10, is spray-dried, the inlet temperature 160 of spray dryer~175 DEG C, leaving air temp 80~85 DEG C, It is ground into powder subsequently, makes Chinese herbal medicine additive;
Step 2: by raw material and the Chinese herbal medicine additive mix homogeneously of residual feed, and use pulverizer to pulverize further Refinement, obtains the feedstuff that particle diameter is 200-250um.
CN201610626288.2A 2016-08-02 2016-08-02 One carp feed in spring Withdrawn CN106173549A (en)

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Publication number Priority date Publication date Assignee Title
CN107252009A (en) * 2017-05-11 2017-10-17 全椒县鮰鱼养殖专业合作社 Yi Zhong Channel-catfish fishes propagate feed artificially
CN107668415A (en) * 2017-11-21 2018-02-09 蚌埠市渔歌农业科技有限公司 A kind of bait for lifting carp meat delicate flavour
CN107801867A (en) * 2017-09-27 2018-03-16 浙江海洋大学 A kind of large yellow croaker composite immune reinforcing agent and preparation method thereof
CN108576494A (en) * 2018-04-28 2018-09-28 广西农垦糖业集团良圻制糖有限公司 A method of producing fish complete feed using sugarcane toppers ammonification
CN110574826A (en) * 2019-08-13 2019-12-17 河南科技大学 Chinese herbal medicine feed additive for preventing koi scale protrusion disease and preparation method thereof

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CN104323038A (en) * 2014-11-13 2015-02-04 张家港耕耘家庭农场有限公司 Carp feed with Chinese herbal medicines
CN105410486A (en) * 2015-12-26 2016-03-23 张旭东 Feed for carp
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CN103141709A (en) * 2013-04-02 2013-06-12 山东新希望六和集团有限公司 Carp feed and preparation method thereof
CN104286596A (en) * 2014-11-11 2015-01-21 济南凯因生物科技有限公司 Feed for carp in breeding season
CN104323038A (en) * 2014-11-13 2015-02-04 张家港耕耘家庭农场有限公司 Carp feed with Chinese herbal medicines
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CN105410486A (en) * 2015-12-26 2016-03-23 张旭东 Feed for carp

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107252009A (en) * 2017-05-11 2017-10-17 全椒县鮰鱼养殖专业合作社 Yi Zhong Channel-catfish fishes propagate feed artificially
CN107801867A (en) * 2017-09-27 2018-03-16 浙江海洋大学 A kind of large yellow croaker composite immune reinforcing agent and preparation method thereof
CN107668415A (en) * 2017-11-21 2018-02-09 蚌埠市渔歌农业科技有限公司 A kind of bait for lifting carp meat delicate flavour
CN108576494A (en) * 2018-04-28 2018-09-28 广西农垦糖业集团良圻制糖有限公司 A method of producing fish complete feed using sugarcane toppers ammonification
CN110574826A (en) * 2019-08-13 2019-12-17 河南科技大学 Chinese herbal medicine feed additive for preventing koi scale protrusion disease and preparation method thereof

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