CN106172083A - A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions - Google Patents
A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions Download PDFInfo
- Publication number
- CN106172083A CN106172083A CN201610554169.0A CN201610554169A CN106172083A CN 106172083 A CN106172083 A CN 106172083A CN 201610554169 A CN201610554169 A CN 201610554169A CN 106172083 A CN106172083 A CN 106172083A
- Authority
- CN
- China
- Prior art keywords
- injection
- amniotic cavity
- embryo egg
- provisions
- method improving
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002347 injection Methods 0.000 title claims abstract description 65
- 239000007924 injection Substances 0.000 title claims abstract description 65
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000011534 incubation Methods 0.000 title claims abstract description 20
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 24
- 230000012447 hatching Effects 0.000 claims abstract description 16
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 12
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 12
- 239000011718 vitamin C Substances 0.000 claims abstract description 12
- 210000002969 egg yolk Anatomy 0.000 claims abstract description 8
- 241000287828 Gallus gallus Species 0.000 claims abstract description 7
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 4
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 239000012188 paraffin wax Substances 0.000 claims description 6
- 241000272522 Anas Species 0.000 claims description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 4
- 108010000912 Egg Proteins Proteins 0.000 claims description 4
- 210000004681 ovum Anatomy 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 238000001354 calcination Methods 0.000 claims description 3
- 239000004575 stone Substances 0.000 claims 1
- 239000001993 wax Substances 0.000 claims 1
- 235000016709 nutrition Nutrition 0.000 abstract description 6
- 230000035764 nutrition Effects 0.000 abstract description 5
- 230000037406 food intake Effects 0.000 abstract description 3
- 235000021050 feed intake Nutrition 0.000 description 5
- 230000037396 body weight Effects 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- 241001508486 Boehmeria longispica Species 0.000 description 2
- 208000029462 Immunodeficiency disease Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 101100279442 Caenorhabditis elegans egg-6 gene Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000002180 anti-stress Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000000994 inner shell membrane Anatomy 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 210000000998 shell membrane Anatomy 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000001325 yolk sac Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K45/00—Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
- A01K45/007—Injecting or otherwise treating hatching eggs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The present invention discloses a kind of method improving incubation rate for embryo egg amniotic cavity injection provisions, injection site selects at embryo egg amniotic cavity, after the Embryo Gallus domesticus absorbing proteins of hatching completes, start to absorb yolk to have absorbed i.e. broken shell to yolk and inject in during this period of time the previous day, to amniotic cavity injection vitamin C.The present invention is applicable to provide nutrition to of the right age embryo egg, thus improves embryo egg hatchability and strong young rate, and, improve the young birth weight of fowl, daily gain (ADG), daily ingestion amount (ADFI), improve feedstuff weightening finish than (F/G).
Description
Technical field
The present invention relates to a kind of method improving incubation rate for embryo egg amniotic cavity injection provisions.
Background technology
Immunodeficiency disease and in a large number stress existence be that the two of poultry large-scale cultivation is challenged greatly.Immunodeficiency disease not only may be used
Make poultry easily infect various disease, cause mortality；Also can be used for more multi-energy maintaining immunity, affect production performance.
Vitamin C is a kind of water soluble vitamins, has functions such as anti-stress, antioxidation and raising immunity, but oxidizable spy
Property make it directly add in diet and drinking-water to be restricted.
Summary of the invention
It is an object of the invention to provide a kind of method improving incubation rate for embryo egg amniotic cavity injection provisions, can give
Of the right age embryo egg provides nutrition, to improve embryo egg hatching rate and point strong young rate.
In order to reach above-mentioned purpose, the solution of the present invention is:
A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions, it is characterised in that: injection site selects at embryo egg
Amniotic cavity, after the Embryo Gallus domesticus absorbing proteins of hatching completes, starts to absorb yolk and has absorbed i.e. broken shell this section the previous day to yolk
Injecting in time, the injection embryo of different fowl egg is different for age, and if egg is at 15 age in days-18 ages in days, Ovum Anas domestica is at 23 ages in days-26
Between age in days, carry out amniotic cavity injection vitamin C, during this period of time because of embryo egg lecithality and embryo, as long as so from air chamber
Punching syringe needle is entered nutritional solution injection the most permissible through inner shell membrane.The concrete operation method of injection is as follows: by according to egg
Method determines amniotic cavity position, sterilizes in blunt end (having the position of air chamber) injection site with 75% alcohol swab, and at sterilising zone
Bore about 1mm aperture, subsequently with the vertical inserting needle of 5mL syringe (degree of depth is about 3cm), injection is expelled in amniotic cavity.
Injection formula: 40% vitamin C；Injection consumption: every piece of embryo egg 0.1ml.
Concrete operation method is as follows: injection and paraffin are injected through autoclaving (about 121 DEG C, about 15min), general
It is consistent that liquid is cooled to hatch built-in temperature.Before injection, determine amniotic cavity by the method according to egg, note at blunt end with 75% alcohol swab
Penetrate position sterilization, and bore about 1mm aperture at sterilising zone, subsequently with the vertical inserting needle of 5ml syringe (degree of depth is about 3cm),
Being expelled in amniotic cavity (0.1ml/ piece) by injection, 1 piece of embryo egg is with 1 piece of syringe needle.After injection, boring sites in curing is used again
75% alcohol swab sterilization, and close drill hole with the paraffin after alcohol burner calcination sterilizing immediately, continue by normal incubation program subsequently
Continuous hatching.
After using such scheme, the present invention is to utilize embryo egg according to egg technology, carries out amniotic cavity injection vitamin C, for poultry
Nutrition regulation opens a brand-new field embryo egg injection nutrition, and the present invention is applicable to provide nutrition to of the right age embryo egg,
Thus improving embryo egg hatchability and strong young rate, the present invention improves the incubation rate of hatching egg and strong young rate more than 1%；Further, significantly carry
The high birth weight of young fowl, daily gain (ADG), daily ingestion amount (ADFI), improve feedstuff and increase weight than (F/G).
Accompanying drawing explanation
Fig. 1 is the inoculation schematic diagram of amniotic cavity.
Label declaration
Shell membrane 1, air chamber 2, allantoic cavity 3, chorioallantoic cavity 4, yolk sac 5, the white of an egg 6, amniotic cavity 7, entry needle 8.
Detailed description of the invention
Shown in reference Fig. 1, a kind of method improving incubation rate for embryo egg amniotic cavity injection provisions of the present invention, injection part
Position selects at embryo egg amniotic cavity 7, after the Embryo Gallus domesticus absorbing proteins of hatching completes, starts to absorb yolk and has absorbed to yolk and i.e. break
Shell is injected in during this period of time the previous day.The concrete operation method of injection is as follows: by injection and paraffin through autoclaving
(about 121 DEG C, about 15min), are cooled to hatch built-in temperature consistent by injection.Before injection, true by the method according to egg
Determine amniotic cavity 7 position, sterilize in blunt end (having the position of air chamber 2) injection site with 75% alcohol swab, and bore 1mm at sterilising zone
Left and right aperture, subsequently with the vertical inserting needle of 5mL entry needle 8 (degree of depth is about 3cm), is expelled to injection in amniotic cavity 7.Injection
Formula of liquid: 40% vitamin C；Injection consumption: every piece of embryo egg 0.1ml.After injection, boring sites in curing is used again 75% wine
The cotton sterilization of essence, and close drill hole with the paraffin after alcohol burner calcination sterilizing immediately, press normal incubation program subsequently and continue hatching.
Embodiment 1: Boehmeria longispica Steud. Ovum Anas domestica hatching treatment effect contrast
Using hatch machine hatching Boehmeria longispica Steud. Ovum Anas domestica, test group uses the inventive method within the 23rd day, to carry out embryo egg injection 40% dimension in hatching
The C process of raw element, matched group is blank, after matched group and injection group duckling all sprout, calculates incubation rate, wherein embryo egg note by repeating groups
(hatch the 23rd day) including not added up by the dead germ detected according to egg before penetrating, result: the test group using the present invention to process is incubated
Change degree improves 1.32%, and strong young rate improves 1.43%.
Embodiment 2:AA+ broiler egg incubation treatment effect contrasts
Using hatch machine hatching mountain AA+ broiler egg, test group uses the inventive method within the 18th day, to carry out embryo egg injection in hatching
40% vitamin C processes, and matched group is blank, after matched group and injection group chickling all sprout, calculates incubation rate by repeating groups, wherein
Including the dead germ that before the injection of embryo egg, (hatching the 18th day) detects by shining egg is not added up, result: use the examination that the present invention processes
Test group hatching degree and improve 1.23%.Strong young rate improves 1.31%.
Embodiment 3: embryo egg injection vitamin C is on the impact that individual growth is grown in early days of AA+ broiler
After going out shell the 1st, 14,28 ages in days by repeating groups record body weight and feed intake, calculate (ADFI) amount of searching for food average day,
Feedstuff weight ratio (F/G).
Table 1 body weight contrast table
The weightening finish of invention group as can be seen from Table 1 is substantially better than the standard body weight of traditional group and this kind.
Table 2 average daily ingestion amount contrast table
The average daily feed intake of invention group as can be seen from Table 2 is higher than traditional group and standard.
3 weeks meat material contrast tables of table
| Project | 1st week feed intake | 2nd week average daily feed intake | 3rd week average daily feed intake |
| Traditional group | 1:1.25 | 1:1.45 | 1:1.60 |
| Invention group | 1:1.21 | 1:1.40 | 1:1.52 |
| Standard feedstuff-meat ratio | 1:1.22 | 1:1.43 | 1:1.54 |
The meat feed ratio of invention group as can be seen from Table 3 is best, and traditional group difference is extremely notable, and criterion group significant difference.
Claims (9)
1. the method improving incubation rate for embryo egg amniotic cavity injection provisions, it is characterised in that: injection site selects at embryo
Egg amniotic cavity, after the Embryo Gallus domesticus absorbing proteins of hatching completes, start to absorb yolk to yolk absorbed i.e. broken shell the previous day this
Inject, to amniotic cavity injection vitamin C in the section time.
A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions, it is characterised in that:
Egg is between 15 age in days-18 ages in days, to amniotic cavity injection vitamin C.
A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions, it is characterised in that:
Ovum Anas domestica is between 23 age in days-26 ages in days, to amniotic cavity injection vitamin C.
A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions, it is characterised in that
The step of injection is as follows:
Step one, first by injection and paraffin through autoclaving, then it is consistent that injection is cooled to hatch built-in temperature；
Step 2, before injection, determines amniotic cavity by the method according to egg, sterilizes in blunt end injection site with 75% alcohol swab, and
Sterilising zone is holed；
Step 3, injection, with the 5 vertical inserting needles of ml syringe, injection is expelled in amniotic cavity；
Step 4, after injection, to boring sites in curing again with 75% alcohol swab sterilization, and with the stone after alcohol burner calcination sterilizing
Seal with wax and close drill hole, press normal incubation program subsequently and continue hatching.
A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions, it is characterised in that
Step one, injection and paraffin are 121 DEG C ± 5 DEG C through autoclaved temperature, and the time is 15 min ± 5min.
A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions, it is characterised in that
Step 2, sterilising zone boring is 1mm ± 0.1min aperture.
A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions, it is characterised in that
Step 3, the vertical depth of needle of syringe is 3cm ± 0.2cm.
A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions, it is characterised in that
Step 3, injection formula is 40% vitamin C, and injection dosage is 0.1 ml/ piece.
A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions, it is characterised in that
Step 3, during injection, 1 piece of embryo egg is with 1 piece of syringe needle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610554169.0A CN106172083A (en) | 2016-07-14 | 2016-07-14 | A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610554169.0A CN106172083A (en) | 2016-07-14 | 2016-07-14 | A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106172083A true CN106172083A (en) | 2016-12-07 |
Family
ID=57474402
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610554169.0A Pending CN106172083A (en) | 2016-07-14 | 2016-07-14 | A kind of method improving incubation rate for embryo egg amniotic cavity injection provisions |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106172083A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111479462A (en) * | 2017-10-31 | 2020-07-31 | 百士立丰有限公司 | Operation control module of guided needle insertion/withdrawal device added to intelligent substance injection device onboard apparatus for inoculating substance into fertilized egg and intelligent method for injection into fertilized egg |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4973595A (en) * | 1989-06-08 | 1990-11-27 | The United States Of America As Represented By The Secretary Of Agriculture | Composition and method for increasing the hatchability of turkey eggs |
| US5158038A (en) * | 1990-06-06 | 1992-10-27 | Sheeks Oliver B | Egg injection method, apparatus and carrier solution for improving hatchability and disease control |
| JP2001078620A (en) * | 1999-09-14 | 2001-03-27 | Keikichi Fujio | Processed sperm shell egg, its production and method for hatching the same |
| TW200514561A (en) * | 2003-07-02 | 2005-05-01 | Bayer Healthcare Ag | Improving hatchability in egg-laying species |
| US20070137577A1 (en) * | 2005-10-05 | 2007-06-21 | Wolfe Stephen P | Methods and apparatus for injecting and sampling material through avian egg membranes |
| EP1385499B1 (en) * | 2001-04-09 | 2008-07-09 | Lonza AG | Use of carnitine for the preparation of an injection solution for preventing or alleviating ascites in poultry by in-ovo injection |
| CN105432565A (en) * | 2016-01-19 | 2016-03-30 | 南京农业大学 | Hatching method for increasing energy reserve of commercial generation hatching eggs of white feather broilers |
-
2016
- 2016-07-14 CN CN201610554169.0A patent/CN106172083A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4973595A (en) * | 1989-06-08 | 1990-11-27 | The United States Of America As Represented By The Secretary Of Agriculture | Composition and method for increasing the hatchability of turkey eggs |
| US5158038A (en) * | 1990-06-06 | 1992-10-27 | Sheeks Oliver B | Egg injection method, apparatus and carrier solution for improving hatchability and disease control |
| JP2001078620A (en) * | 1999-09-14 | 2001-03-27 | Keikichi Fujio | Processed sperm shell egg, its production and method for hatching the same |
| EP1385499B1 (en) * | 2001-04-09 | 2008-07-09 | Lonza AG | Use of carnitine for the preparation of an injection solution for preventing or alleviating ascites in poultry by in-ovo injection |
| TW200514561A (en) * | 2003-07-02 | 2005-05-01 | Bayer Healthcare Ag | Improving hatchability in egg-laying species |
| US20070137577A1 (en) * | 2005-10-05 | 2007-06-21 | Wolfe Stephen P | Methods and apparatus for injecting and sampling material through avian egg membranes |
| CN105432565A (en) * | 2016-01-19 | 2016-03-30 | 南京农业大学 | Hatching method for increasing energy reserve of commercial generation hatching eggs of white feather broilers |
Non-Patent Citations (2)
| Title |
|---|
| 李德生等: "胚蛋注射蛋氨酸对朗德鹅生产性能和血液生化指标的影响", 《中国畜牧杂志》 * |
| 马翠然: "维生素对种蛋孵化率的影响", 《养禽与禽病防治》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111479462A (en) * | 2017-10-31 | 2020-07-31 | 百士立丰有限公司 | Operation control module of guided needle insertion/withdrawal device added to intelligent substance injection device onboard apparatus for inoculating substance into fertilized egg and intelligent method for injection into fertilized egg |
| CN111479462B (en) * | 2017-10-31 | 2023-03-14 | 百士立丰有限公司 | Intelligent injection device, operation control module and intelligent injection method |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: Longyan City, Fujian province Denggao road 364000 Xinluo District No. 688 Applicant after: Fujian Zhengda Food Co., Ltd. Applicant after: Longyan University Address before: Longyan City, Fujian province Denggao road 364000 Xinluo District No. 688 Applicant before: Fujian Sumpo Food Group Co., Ltd. Applicant before: Longyan University |
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| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20161207 |