CN106171582A - Induction fruit resistance controls the method for disease and preparation used - Google Patents
Induction fruit resistance controls the method for disease and preparation used Download PDFInfo
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- CN106171582A CN106171582A CN201610516654.9A CN201610516654A CN106171582A CN 106171582 A CN106171582 A CN 106171582A CN 201610516654 A CN201610516654 A CN 201610516654A CN 106171582 A CN106171582 A CN 106171582A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
- A23B7/155—Microorganisms; Enzymes; Antibiotics
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- Storage Of Fruits Or Vegetables (AREA)
Abstract
The invention discloses a kind of preparation inducing fruit resistance to control disease, containing 1 × 10 in 1L preparation9～1 × 1010Individual saccharomyces cerevisiae inactivated bacteria somatic cell, remaining is water.The present invention provides the method that the induction fruit resistance utilizing above-mentioned preparation to carry out controls disease the most simultaneously, before fruit vanning storage, first carry out the pretreatment of any one mode following: pretreatment mode one, first put into by fruit in preparation and soak, put into container after draining and place under sealing state 23～25 hours；Before pretreatment mode two, picking fruit, preparation is sprayed on fruit surface, places 23～25 hours under sealing state in being then placed in container；Case again after above-mentioned pretreated fruit is taken out from container.The present invention is used can effectively to control postharvest diseases of fruit on the premise of can realizing not using chemical bactericide.
Description
Technical field
The present invention relates to postharvest diseases of fruit Prevention Technique field, more particularly a kind of by inducing fruit resistance to press down
The biological way of keeping fresh of postharvest diseases of fruit processed.
Background technology
The loss that fruit causes owing to rotting after adopting is the hugest, and according to data, the annual fruit and vegerable of China rot super
Crossing 80,000,000 tons, the economic loss caused reaches hundred billion yuan.It is cause fruit postharvest decay the most former that pathogenic fungi infects
Cause, common pathogenic fungi mainly has Penicillium (Penicillum spp.), Botrytis (Botrytis spp.) and chain lattice
Spore belongs to (Alternaria spp.) etc..
At present, chemical bactericide remains the major measure controlling fruit postharvest diseases.As a example by Citrus, Chinese invention is special
Profit (application number 200910028802.2, citrus preservative) provides the multiple chemical bactericide that can control citrus disease, including
Thiophanate methyl, carbendazim.Chinese invention patent (application number 200910028804.1, long-acting preservative for quince) provides controlled
The chemical bactericide of citrus disease processed, including 2,4-D sodium salt, thiophanate.Chinese invention patent (application number
201110209305.X, a kind of citrus preservative and preparation method thereof, application) provide the multiple chemistry that can control citrus disease
Antibacterial, including kresoxim-methyl, azoles bacterium amine fat, trifloxystrobin, Enestroburin etc..But, the residual of chemical bactericide is easily to human body
Healthy and Environmental security causes serious harm, pathogenic microorganism can be caused simultaneously to produce drug resistance, affect disease-controlling effect.Cause
This, find novel, safely and effectively Disease management method to be to replace antibacterial to be increasingly becoming focus of concern.
Plant self also exists the defense mechanism of a set of complexity to resist the invasion of pathogen.Exogenous factor can be by thorn
Swashing plant tissue, make this defense mechanism show, so that plant produces disease resistance, this phenomenon is referred to as induction of resistance.
Numerous studies show, heat treatment, ultraviolet, Calcium treatment, phytohormone and natural bacteriostatic material, biological preservative etc. can be induced
Fruit produces resistance.Induction of resistance, as a kind of prevention and controls utilizing plant self disease resistance mechanisms, is compared with chemical bactericide
There is safe and stable, advantages of environment protection, it is possible to rotting it is considered to be have in storage after effectively reducing Fruit
One of novel Disease management method of effect.China authorizes patent of invention " mixture of induction of resistance and method in plant ", public
The number of opening 1925748, discloses the two kinds or more of compounds of a kind of use, including salicylic acid or its function similar products, promotion
Property compound, modulating compound, by stimulate natural plant system of defense and in plant induction of resistance suppress pathogen
The method infected.Chinese invention patent (application number 201510015741.1, a kind of muskmelon disease control method) provides one
The muskmelon disease control method of safety, i.e. forms phase and before adopting 48 hours four at young Fructus Melo phase, the rapid expanding stage of fruit, reticulate pattern
Individual period sprays aspirin, to induce the resistance of fruit.Chinese invention patent (application number 201410314131.7, induction
Fruit resistance controls the method for disease and preparation used) provide one and utilize tobacco brown spot pathogen solution induction fruit resistance with control
The method of disease processed.
Saccharomyces cerevisiae (Saccharomyces cerevisiae) is biological species the most frequently used in fermented product, at food
The every field of industry is all widely used.China authorizes patent of invention " a kind of oligo-glucomannan rich in saccharomyces cerevisiae
The preparation method of feed additive ", publication number 102048029A, disclose a kind of oligo-glucomannan and saccharomyces cerevisiae of comprising
Animal feed additive, and provide the preparation method of this feed additive.Chinese invention patent (application number
A kind of 201610044689.7, method utilizing mixed fungus fermentation brew pear wine) disclose one and utilize saccharomyces cerevisiae and abnormal the most complete
The method of red culture propagation brew pear wine.Chinese invention patent (application number 201610129186.X, an Accharomyces cerevisiae and should
With) disclose an Accharomyces cerevisiae, it is applied to Lattice Topology traditional zymotic production process, there is shortening fermentation period, acidproof
Property good, can be with the feature of lactic acid bacteria co-fermentation.(application number 201310648466.8, saccharomyces cerevisiae exists Chinese invention patent
The application of fruit postharvest diseases preventing and treating and using method) disclose a strain there is the Wine brewing yeast strain of biological and ecological methods to prevent plant disease, pests, and erosion effect, and provide
Utilize the method that this bacterial strain carries out fruit postharvest diseases preventing and treating and preservation and freshness.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of preparation inducing fruit resistance to control disease, uses the present invention
Postharvest diseases of fruit can be effectively controlled on the premise of can realizing not using chemical bactericide.
In order to solve above-mentioned technical problem, the present invention provides a kind of preparation inducing fruit resistance to control disease, 1L preparation
In containing 1 × 109～1 × 1010Individual saccharomyces cerevisiae inactivated bacteria somatic cell, remaining is water (sterile distilled water).
Improvement as the preparation inducing fruit resistance control disease of the present invention: by saccharomyces cerevisiae somatic cells in 121
DEG C sterilizing 20min, obtains saccharomyces cerevisiae inactivated bacteria somatic cell.
The further of preparation as the induction fruit resistance control disease of the present invention is improved: containing 1 × 10 in 1L preparation10
Individual saccharomyces cerevisiae inactivated bacteria somatic cell, remaining is water (sterile distilled water).
The present invention provides the method that the induction fruit resistance utilizing above-mentioned preparation to carry out controls disease the most simultaneously, at fruit
Before vanning storage, first carry out the pretreatment of any one mode following:
Pretreatment mode one,
First fruit is put in preparation and soak, put into container after draining and place under sealing state 23～25 hours；
Before pretreatment mode two, picking fruit, preparation is sprayed on fruit surface (moistening), be then placed in container in
Place 23～25 hours under sealing state；
Case again after above-mentioned pretreated fruit is taken out from container.
Improvement as the method inducing fruit resistance control disease of the present invention:
In described pretreatment mode one, soak time is 8～12 minutes (for example, 10 minutes).
Further improvements in methods as the induction fruit resistance control disease of the present invention:
Container in described pretreatment mode one and pretreatment mode two is antistaling film bag.
In the present invention, disease for example, penicilliosis, melasma.
It is common that Saccharomyces Cerevisiae in S accharomyces cerevisiae is purchased from China Committee for Culture Collection of Microorganisms
Microorganism center, bacterium numbering is 2.3854.
In the present invention,
Pretreatment mode one: select to have no mechanical damage, be uninfected by, fruit that the exterior quality such as size and Maturity is basically identical
Real, rinse with tap water and be allowed to dry under room temperature (about 25 DEG C).Then the fruit after cleaning soaks in the preparation of the present invention
After steeping 8～12 minutes (preferably 10 minutes), take out and be allowed to dry (that is, draining the water), place in antistaling film bag (tighten bag mouth,
It is made to seal) under room temperature (about 25 DEG C), keep 23～25 hours (that is, about 24 hours), then fruit is taken from bag film
Go out, vanning storage.
Processing mode two: adopt the method process that front watering can sprays, before picking fruit, the preparation of the present invention is sprayed on
Fruit surface (moistens), and after dry, (after fruit surface no longer dropping liquid) takes off fruit and put in antistaling film bag and (tighten bag
Mouthful so that it is seal) under room temperature (about 25 DEG C), keep 23～25 hours (that is, about 24 hours), then by fruit from bag film
Middle taking-up, vanning storage.
The preparation method of the saccharomyces cerevisiae inactivated bacteria liquid suspension in the present invention is:
Saccharomyces cerevisiae be saved under low temperature (4 DEG C) NYDA culture medium (beef extract 8g, yeast powder 5g, glucose 10g,
Agar 20g, is settled to 1000mL, high pressure steam sterilization, 121 DEG C of sterilizing 20min with water) in, during activation take out, at 28 DEG C in
NYDA culture medium is cultivated 48 hours (condition of culture is: 200rpm, 28 DEG C), after repeating Secondary Culture 2 times, will live with inoculating loop
NYDB culture medium received by the yeast changed, and (beef extract 8g, yeast powder 5g, glucose 10g, be settled to 1000mL, high pressure with water
Steam sterilization, 121 DEG C of sterilizing 20min) in, cultivate 24 hours at 200rpm, under the conditions of 28 DEG C, collect culture fluid,
4000rpm, centrifugal 15min under the conditions of 4 DEG C, wash 2 times with sterile distilled water, to remove culture medium.Use sterile distilled water weight
New suspended yeast cell, determines its concentration (available sterile distilled water is adjusted to required concentration) with blood cell counting plate, finally
121 DEG C of sterilizing 20min in high-pressure steam sterilizing pan, obtain saccharomyces cerevisiae inactivated bacteria liquid suspension.By saccharomyces cerevisiae inactivated bacteria
Liquid suspension sterile distilled water is diluted, until saccharomyces cerevisiae inactivated bacteria somatic cell is 1 × 1010～1 × 1011Individual/L,
Induction fruit resistance controls the preparation of disease.
The invention have the advantage that the saccharomyces cerevisiae aboundresources in (1) present invention, with low cost, cost performance is high, has life
Biodegradable；(2) induction of resistance is a kind of natural reaction produced after plant is infected by pest and disease damage, can produce pest and disease damage
Persistently and the resistance of wide spectrum of system；(3) postharvest disease of fruit can be significantly reduced, environmental and human health impacts is made without any murder by poisoning
With, there is the features such as economical and practical, safe and efficient, environmental friendliness.(4) said preparation energy on the premise of not using chemical bactericide
Effectively control postharvest diseases of fruit.(5), the somatic cells after inactivation treatment is more convenient for preserving.
Accompanying drawing explanation
Below in conjunction with the accompanying drawings the detailed description of the invention of the present invention is described in further detail.
Fig. 1 is the inactivation saccharomyces cerevisiae thalline inducing action comparison diagram to pear fruit penicilliosis resistance；
Wherein figure (a) is sickness rate, and figure (b) is lesion diameter.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This.
It is common that Saccharomyces Cerevisiae in S accharomyces cerevisiae is purchased from China Committee for Culture Collection of Microorganisms
Microorganism center, bacterium numbering is 2.3854.
Embodiment 1, for controlling the preparation of postharvest diseases of fruit
The concentration of saccharomyces cerevisiae is adjusted to 1 × 108Cell/mL, after 121 DEG C of sterilizing 20min, obtains saccharomyces cerevisiae inactivated bacteria
Liquid suspension.Take 1L saccharomyces cerevisiae inactivated bacteria liquid suspension, add sterilized water and be diluted.Until every 1L preparation of gained contains
1×109-1×1010Individual inactivated bacteria somatic cell, remaining is water (sterile distilled water)；Fruit resistance must be induced to control the system of disease
Agent.
In order to prove the practical effect of invention formulation, inventor has carried out following experiment:
Experiment 1, the inactive yeast thalline inducing action to pear fruit penicilliosis resistance
1, experiment material:
Fruit is Shuijing Pear.
Pathogen: penicillium expansum (Penicillium expansum), 25 DEG C activation 7 days standby.
2, process:
Choose outward appearance neat, without pest and disease damage, the fruit that has no mechanical damage, first with tap water cleaning, immerse 0.1% the most again
The liquor natrii hypochloritis of (quality %) sterilizes 2 minutes, takes out, then rinse well with tap water, wash away remaining sodium hypochlorite, dry in the air
Dry standby.Form the wound of unified size and the degree of depth at each fruit real surface with sterilized card punch.Each wound adds
30 μ L concentration are respectively 1 × 105、1×106、1×107、1×108The saccharomyces cerevisiae inactivated bacteria liquid suspension of cell/mL, to add
Enter the sterilized water of equivalent as comparison；And use PE plastic film seal.Raise PE plastic foil after 24 hours to add in each wound
(Penicillium expansum, concentration is 1 × 10 to 30 μ L pathogen spore suspension4Spores/mL), room temperature (25 DEG C
Left and right) under preserve, again go bail for wet process with PE plastic film seal.Each process is repeated 3 times, 9 fruits of each repetition, experiment
It is repeated twice, is as the criterion with identical result.3rd day observed result.
3, result:
As it is shown in figure 1, matched group is accessing after pathogen the 3rd day, sickness rate reaches 96.3%, and lesion diameter reaches
10.1mm。1×105With 1 × 106The saccharomyces cerevisiae inactivated bacteria liquid suspension of cell/mL and does not all have on lesion diameter on sickness rate
There is the effect significantly inhibiting pear fruit penicilliosis；1×107The inactivated bacteria liquid suspension of cell/mL compares under comparison on sickness rate
Fall 29.6%, declines 4.0mm on lesion diameter, has significant inhibition；And 1 × 108The suspension of cell/mL is in morbidity
Do not embody inhibition in rate, on lesion diameter, only have inhibition, and inhibition is not as 1 × 107Cell/mL
Group.
Experiment 2, the inactive yeast thalline inducing action to Cherry Tomato Fruit melasma resistance
1, experiment material:
Fruit is cherry tomato.
Pathogen: rod method (Alternaria alternata), 25 DEG C activation 14 days standby.
2, process:
Choose outward appearance neat, without pest and disease damage, the fruit that has no mechanical damage, first with tap water cleaning, immerse 0.1% the most again
Liquor natrii hypochloritis in sterilize 2 minutes, take out, then rinse well with tap water, wash away remaining sodium hypochlorite, dry standby.
Cherry Tomato Fruit is immersed in 1 × 10710min in the saccharomyces cerevisiae inactivated bacteria liquid suspension of cell/mL, with nothing
Bacterium water, as comparison, takes out and is allowed to dry (draining) afterwards with the sealing of PE plastic sheeting.After 24 hours, with sterilized card punch each
Cherry Tomato Fruit surface forms the wound of unified size and the degree of depth, adds 20 μ L A.alternata spore suspension, concentration
It is 1 × 104spores/mL.Timing every day is observed, a situation arises for record fruit wound disease, and result is with average attack rate
(%) represent.Each process is repeated 3 times, and 20 fruits of each repetition, experiment is repeated twice, and is as the criterion with identical result.72 hours
Rear observed result.
3, result:
As shown in table 1, with 1 × 107Cherry Tomato Fruit is carried out whole by the saccharomyces cerevisiae inactivated bacteria liquid suspension of cell/mL
Really immersion treatment, accesses pathogen after 24 hours again, and inactive yeast thalline is compared matched group sickness rate and be have dropped 16.9%, tool
There were significant differences.
The sickness rate (%) of Cherry Tomato Fruit after table 1, induction 24 hours
| The sickness rate of 72 hours | |
| Sterilized water | 90.2±3.0 |
| l×l07Individual/mL inactivates thalline | 73.3±4.1 |
Contrast experiment 1, by described in experiment 2 " after 24 hours, with sterilized card punch at each cherry tomato really
Real surface forms the wound of unified size and the degree of depth " change into " behind 0 hour, 6 hours and 48 hours, beating with sterilized
Hole device forms the wound of unified size and the degree of depth on each Cherry Tomato Fruit surface ", saccharomyces cerevisiae inactivation thalline used hangs
The concentration of supernatant liquid remains as 1 × 107Individual/mL；Remaining is equal to test 2.
The result observed for 72nd hour is as described in Table 2:
The sickness rate (%) of Cherry Tomato Fruit under table 2, different induction time
Under conditions of not induction (0 hour), short time induction (6 hours), long-time induction (48 hours), ferment of making wine
Female inactivation thalline is all not so good as the present invention (induction time is 24 hours) to the inhibition of Cherry Tomato Fruit melasma.Wherein exist
Under conditions of not induction, short time induction, inactive yeast thalline can not embody inhibitory action.
Comparative example,
Bacillus subtilis (Bacillus subtilis) is to Penicillium italicum (Penicillium italicum) and green
Mildew (Penicillium digitatum), and the brown rot (Monilinia to fruits such as Fructus Pruni pseudocerasi, yellow peach, Prunus persicanucipersica Schneider
Fructicola), Semen Castaneae adopts rear melasma etc. good inhibition.But after being inactivated by bacillus subtilis, it is not
Possesses inhibition again, even if as of the present invention fruit being induced that (induction time is set as 1～48 hour, concentration
It is set as 1 × 106/ L～1 × 1012/ L), the most do not possess inhibition.
Finally, in addition it is also necessary to be only several specific embodiments of the present invention it is noted that listed above.Obviously, this
Bright it is not limited to above example, it is also possible to have many deformation.Those of ordinary skill in the art can be from present disclosure
The all deformation directly derived or associate, are all considered as protection scope of the present invention.
Claims (6)
1. induction fruit resistance controls the preparation of disease, it is characterized in that: containing 1 × 10 in 1L preparation9～1 × 1010Individual wine brewing ferment
Female inactivated bacteria somatic cell, remaining is water.
Induction fruit resistance the most according to claim 1 controls the preparation of disease, it is characterized in that: by thin for saccharomyces cerevisiae thalline
Born of the same parents, in 121 DEG C of sterilizing 20min, obtain saccharomyces cerevisiae inactivated bacteria somatic cell.
Induction fruit resistance the most according to claim 2 controls the preparation of disease, it is characterized in that: in 1L preparation containing 1 ×
1010Individual saccharomyces cerevisiae inactivated bacteria somatic cell, remaining is water.
4. the method that the induction fruit resistance utilizing the preparation as described in claims 1 to 3 is arbitrary to carry out controls disease, its feature
It is: before fruit vanning storage first to carry out the pretreatment of any one mode following:
Pretreatment mode one,
First fruit is put in preparation and soak, put into container after draining and place under sealing state 23～25 hours；
Before pretreatment mode two, picking fruit, preparation is sprayed on fruit surface, places under sealing state in being then placed in container
23～25 hours；
Case again after above-mentioned pretreated fruit is taken out from container.
Induction fruit resistance the most according to claim 4 controls the method for disease, it is characterized in that:
In described pretreatment mode one, soak time is 8～12 minutes.
6. the method controlling disease according to the induction fruit resistance described in claim 4 or 5, is characterized in that:
Container in described pretreatment mode one and pretreatment mode two is antistaling film bag.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610516654.9A CN106171582B (en) | 2016-06-28 | 2016-06-28 | Method for controlling diseases by inducing fruit resistance and preparation used by method |
Applications Claiming Priority (1)
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| CN201610516654.9A CN106171582B (en) | 2016-06-28 | 2016-06-28 | Method for controlling diseases by inducing fruit resistance and preparation used by method |
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| Publication Number | Publication Date |
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| CN106171582A true CN106171582A (en) | 2016-12-07 |
| CN106171582B CN106171582B (en) | 2019-12-31 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107881121A (en) * | 2017-12-15 | 2018-04-06 | 北京工商大学 | The saccharomyces cerevisiae BY23 and its preparation and application method of one plant of control fruit postharvest diseases |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102333857A (en) * | 2009-01-27 | 2012-01-25 | 乐斯福公司 | Saccharomyces cerevisiae strains with phytosanitary capabilities |
| CN103820343A (en) * | 2013-12-04 | 2014-05-28 | 江苏大学 | Application of saccharomyces cerevisiae in disease prevention and treatment on postharvest fruits and use method thereof |
| CN104161116A (en) * | 2014-07-04 | 2014-11-26 | 浙江大学 | Preparation for controlling diseases by inducing fruit resistibility and corresponding induction method |
-
2016
- 2016-06-28 CN CN201610516654.9A patent/CN106171582B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102333857A (en) * | 2009-01-27 | 2012-01-25 | 乐斯福公司 | Saccharomyces cerevisiae strains with phytosanitary capabilities |
| CN103966111A (en) * | 2009-01-27 | 2014-08-06 | 乐斯福公司 | Saccharomyces cerevisiae strains with phytosanitary capabilities |
| CN103820343A (en) * | 2013-12-04 | 2014-05-28 | 江苏大学 | Application of saccharomyces cerevisiae in disease prevention and treatment on postharvest fruits and use method thereof |
| CN104161116A (en) * | 2014-07-04 | 2014-11-26 | 浙江大学 | Preparation for controlling diseases by inducing fruit resistibility and corresponding induction method |
Non-Patent Citations (2)
| Title |
|---|
| DA FU 等: "Effect of β-glucan on stress tolerances and biocontrol efficacy of Cryptococcus laurentii against Penicillium expansum in pear fruit", 《BIOCONTROL》 * |
| 张梦月 等: "植物诱导抗病性诱导剂研究进展", 《中国线虫学研究》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107881121A (en) * | 2017-12-15 | 2018-04-06 | 北京工商大学 | The saccharomyces cerevisiae BY23 and its preparation and application method of one plant of control fruit postharvest diseases |
| CN107881121B (en) * | 2017-12-15 | 2021-11-09 | 北京工商大学 | Saccharomyces cerevisiae BY23 for controlling postharvest diseases of fruits and preparation and use methods thereof |
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| CN106171582B (en) | 2019-12-31 |
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