CN103999930A - Bio-composite anti-mold preservative for citrus, and preparation method and using method thereof - Google Patents
Bio-composite anti-mold preservative for citrus, and preparation method and using method thereof Download PDFInfo
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- CN103999930A CN103999930A CN201410242839.6A CN201410242839A CN103999930A CN 103999930 A CN103999930 A CN 103999930A CN 201410242839 A CN201410242839 A CN 201410242839A CN 103999930 A CN103999930 A CN 103999930A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 16
- 235000020971 citrus fruits Nutrition 0.000 title abstract description 15
- 239000003755 preservative agent Substances 0.000 title abstract description 7
- 230000002335 preservative effect Effects 0.000 title abstract description 7
- 241000207199 Citrus Species 0.000 title abstract 13
- 239000011173 biocomposite Substances 0.000 title abstract 3
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 42
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 38
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000012153 distilled water Substances 0.000 claims abstract description 19
- 239000011780 sodium chloride Substances 0.000 claims abstract description 19
- 229960000583 acetic acid Drugs 0.000 claims abstract description 18
- 239000012362 glacial acetic acid Substances 0.000 claims abstract description 16
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 11
- 229920000053 polysorbate 80 Polymers 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims abstract description 8
- 201000010099 disease Diseases 0.000 claims abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 7
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 4
- 239000004033 plastic Substances 0.000 claims abstract description 4
- 229920003023 plastic Polymers 0.000 claims abstract description 4
- 239000008223 sterile water Substances 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 241000675108 Citrus tangerina Species 0.000 claims description 57
- 239000012530 fluid Substances 0.000 claims description 43
- 239000003795 chemical substances by application Substances 0.000 claims description 38
- 239000002131 composite material Substances 0.000 claims description 31
- 235000015097 nutrients Nutrition 0.000 claims description 15
- 235000013399 edible fruits Nutrition 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 11
- 239000004698 Polyethylene Substances 0.000 abstract description 6
- 238000003860 storage Methods 0.000 abstract description 4
- -1 polyethylene Polymers 0.000 abstract description 3
- 229920000573 polyethylene Polymers 0.000 abstract description 3
- 229920001661 Chitosan Polymers 0.000 abstract 2
- 239000005708 Sodium hypochlorite Substances 0.000 abstract 2
- 238000000855 fermentation Methods 0.000 abstract 2
- 230000004151 fermentation Effects 0.000 abstract 2
- 238000004806 packaging method and process Methods 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 1
- 239000008399 tap water Substances 0.000 abstract 1
- 235000020679 tap water Nutrition 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 10
- 238000004321 preservation Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 102000003992 Peroxidases Human genes 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 229960005070 ascorbic acid Drugs 0.000 description 5
- 235000010323 ascorbic acid Nutrition 0.000 description 5
- 239000011668 ascorbic acid Substances 0.000 description 5
- 108040007629 peroxidase activity proteins Proteins 0.000 description 5
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- 238000012360 testing method Methods 0.000 description 5
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- 230000001046 anti-mould Effects 0.000 description 4
- 239000002546 antimould Substances 0.000 description 4
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a bio-composite anti-mold preservative for citrus. The bio-composite anti-mold preservative for the citrus disclosed by the invention consists of the following raw materials in part by weight and volume: 1 part of glacial acetic acid, 2 to 5 parts of chitosan, 1.5 to 2 parts of bacillus amyloliquefaciens HRH.317 fermentation liquid, 1.5 to 2 parts of Tween-80, 0.3 to 0.5 part of NaCl, and 93 parts of distilled water. The preparation method of the preservative comprises the following steps: firstly, adding glacial acetic acid into the distilled water to prepare acetic acid solution; then, respectively adding the chitosan, the bacillus amyloliquefaciens HRH.317 fermentation liquid, the Tween-80 and the NaCl into the acetic acid solution; and finally, stirring the mixture uniformly. The using method of the preservative comprises the following steps: washing the citrus without damage or disease with tap water, and immersing the citrus into 0.1 percent sodium hypochlorite solution and sterilizing for 1 to 2 minutes, taking the citrus out, cleaning the sodium hypochlorite adhered to the citrus by using sterile water, drying the citrus, completely immersing the citrus into the preservative for 30 to 40 minutes, fishing out the citrus, and packaging the citrus by using PE (polyethylene) freshness protection bags; packaging 25 to 40 citruses in each bag; arranging the packaged citruses in plastic baskets for storage under the conditions that the room temperature is 18 to 23 DEG C and the indoor relative humidity is 70 to 80 percent, or under the conditions that the storage temperature is 4 to 6 DEG C and the relative humidity is 70 to 80 percent.
Description
Technical field
The present invention relates to oranges and tangerines preservation technique field, be specifically related to adopt biological anti-mould fresh keeping agent oranges and tangerines to be carried out to the preparation and application of fresh-keeping and anti-mould fresh keeping agent.
Background technology
Fruits and vegetables are one of Major Foods of the mankind, but fruits and vegetables are subject to regional and seasonal impact, easily occur putrid and deteriorated.The putrid and deteriorated meeting of fruits and vegetables causes unnecessary monetary loss, and causes the reduction of fruit-vegetable quality, and wherein food-borne bacterium can produce a large amount of toxin, makes people cause the diseases such as disease of digestive tract, immunological diseases and inflammation.Traditional chemical anti-mildew fresh-keeping liquid is toxic large, easily cause people's allergy and poisoning, and novel biological preservation liquid is only albumen, polypeptide, some lipid and organic acid etc., to human body do not have toxic and side effect or toxic and side effect very little.Biological preservation liquid is the material that a class of direct separation and Extraction from biology has bacteriostasis, can be divided into three types, i.e. animal sources natural fresh-keeping liquid, vegetable source natural fresh-keeping liquid and microbial source natural fresh-keeping liquid according to source.Therefore, the research and development of the effective biological fruit and vegetable anti-mildew fresh-keeping of new type of safe liquid are extremely urgent.
Summary of the invention
The object of the invention is the deficiency to the research and development of biological preservation liquid for prior art, the biological composite mildewproof antistaling agent of a kind of oranges and tangerines and preparation and application are provided.
The biological composite mildewproof antistaling agent of oranges and tangerines of the present invention is comprised of following quality, parts by volume raw material: 1 part, glacial acetic acid, and shitosan 2-3 part, bacillus amyloliquefaciens (
bacillus amyloliquefaciens) HRH.317 zymotic fluid 1.5-2 part, Tween-80 1.5-2 part, NaCl 0.3-0.5 part, 93 parts of distilled water.
The preparation method of the biological composite mildewproof antistaling agent of oranges and tangerines of the present invention comprises the steps:
(1) in 93 parts of distilled water, add the glacial acetic acid of 1 part (V/V) to make acetum;
(2) in the acetum of preparing in (1), add respectively the shitosan of 2-3 part (W/V), the Tween-80 of the bacillus amyloliquefaciens HRH317 zymotic fluid of 1.5-2 part (V/V), 1.5-2 part (W/V) and the NaCl of 0.3-0.5 part (W/V) stir.
The biological composite mildewproof antistaling agent of oranges and tangerines of the present invention is colourless or slightly light yellow transparent liquid.
Described bacillus amyloliquefaciens (
bacillus amyloliquefaciens) HRH.317 bacterial strain Yi You Agricultural University Of Shanxi is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms and apply for a patent, preserving number is CGMCC JL/0136, number of patent application is 201310187052.X.
The preparation method of described bacillus amyloliquefaciens HRH.317 zymotic fluid comprises the steps:
(1) prepare fluid nutrient medium and in 100 parts of distilled water, add 0.5 portion of beef extract, 1 part of peptone and 0.5 part of NaCl, regulate after pH to 7.2, sterilizing in 121 ℃, 20 minutes, makes nutrient solution standby;
(2) activation of bacillus amyloliquefaciens HRH317 is under aseptic condition, bacillus amyloliquefaciens HRH.317 is inoculated in the nutrient solution of (1) preparing with oese, under 37 ℃ of conditions, rotating speed is shaken cultivation 24 h in the constant-temperature table of 150 r/min, standby as seed liquor;
(3) seed liquor (2) having been activated, in the fluid nutrient medium of preparing with 1% (V/V) inoculum concentration access (1), at rotating speed, be shaken cultivation 24 h in the constant-temperature table of 150 r/min, obtain bacillus amyloliquefaciens HRH.317 basis zymotic fluid;
(4) in fluid nutrient medium prepared by the basic zymotic fluid access (1) of being prepared by (3), under 36-38 ℃, 160 r/min conditions, cultivate 20-24 h, expand to cultivate and obtain zymotic fluid, zymotic fluid viable count is 10
7-10
8cfu/mL.
Bacillus amyloliquefaciens HRH.317 zymotic fluid experimental results show that it is safe through the anxious poison of animal used as test.
The using method of the biological composite mildewproof antistaling agent of oranges and tangerines of the present invention comprises the steps:
(1) by without withered, not damaged, disease-free oranges and tangerines, after cleaning with running water, immerse the 1-2 min that sterilizes in 0.1% liquor natrii hypochloritis, the clorox of bearing fruit by sterile water wash after taking-up, and dry;
(2) oranges and tangerines after drying are immersed completely to biological composite mildewproof antistaling agent of the present invention, soak 30-40 second, pull out dry completely rear with thickness be 0.2-0.4 mm PE (polyethylene) freshness protection package (in advance on bag at interval of 15 cm places punchings, bore dia is 1 cm) packing, every bag of 25-40 fruit, stay 1/4 sack space, put under the condition that plastic crate is built in room temperature 18-23 ℃ and indoor relative humidity 70%-80% or be placed under reserve temperature 4-6 ℃, the condition of relative humidity 70%-80% and preserve.
The anti-mildew fresh-keeping effect test of the biological composite mildewproof antistaling agent of oranges and tangerines of the present invention
1 test method
1.1 test materials: 400 of the intact oranges and tangerines of crust.
1.2 processing methods: A processes the intact oranges and tangerines of crust with biological composite mildewproof antistaling agent of the present invention, and processing method is with above-mentioned using method; B contrast 1 is the oranges and tangerines that crust is intact; C processes and manually punctures the citrus fruit peel inoculation mould spore that crust is intact with biological composite mildewproof antistaling agent of the present invention, and processing method is with above-mentioned using method; D contrast 2 is for manually puncturing the citrus fruit peel inoculation mould spore that crust is intact; Each 100 fruits for the treatment of capacity.
Due at present for whether using chemical anti mildew agent also there is no unified final conclusion on oranges and tangerines, distributors has been used chemical anti mildew agent all to take avoid or do not take a stand to the oranges and tangerines of selling on market simultaneously, therefore, under the prerequisite of not knowing mould dose of kind of concrete chemical preservation and working concentration, be difficult to determine a suitable chemical anti mildew agent contrast.
1.3 holding conditions and time: A holding conditions is temperature 4-6 ℃, relative humidity 70%-80%, 60 days time; B holding conditions is 18-23 ℃, relative humidity 70%-80%, 20 days time; C holding conditions is temperature 18-21 ℃, relative humidity 70%-80%, 15 days time.
1.4: the mould variability of oranges and tangerines, peroxidase content, mda content, weight-loss ratio, soluble solid content, titratable acid content, ascorbic acid content.
2 result of the tests
2.1 holding conditions are temperature 4-6 ℃, relative humidity investigation index 70%-80%, while preserving 60 days, the mould variability of processing oranges and tangerines through anti-mould fresh keeping agent of the present invention is 9%, and the mould variability of oranges and tangerines of contrast 1 is 28%, processes than contrast 1 and makes the mould variability of oranges and tangerines reduce by 19 percentage points; Holding conditions is 18-23 ℃, relative humidity 70%-80%, and while preserving 20 days, the mould variability of processing oranges and tangerines through anti-mould fresh keeping agent of the present invention is 14%, and the mould variability of oranges and tangerines of contrast 1 is 40%, processes than contrast 1 and makes the mould variability of oranges and tangerines reduce by 26 percentage points.
2.2 holding conditions are temperature 18-21 ℃, relative humidity 70%-80%, and during 15 days time, the mould variability of oranges and tangerines that C processes is 35%, and the mould variability of oranges and tangerines of contrast 2 is 100%, processes than contrast 2 and makes the mould variability of oranges and tangerines reduce by 65 percentage points.And process the peroxidase content 12.2 Δ OD of C oranges and tangerines
470/ g.Fw.min, mda content 21.5 mmol/g Fw, weight-loss ratio 2.2%, soluble solid content 9.6%, titratable acid content 0.61%, ascorbic acid content 13.7 mg/100g; And the peroxidase content 7.7 Δ OD of contrast 2 oranges and tangerines
470/ g.Fw.min, mda content 25.4mmol/g Fw, weight-loss ratio 4.1%, soluble solid content 8.8%, titratable acid content 0.53%, ascorbic acid content 11.6 mg/100g.Wherein, the peroxidase of high-load represents the high resistance to cold and diseases of oranges and tangerines, and the MDA of low content represents that oranges and tangerines degree of spoilage is low, and oranges and tangerines can be preserved the longer time; The weight-loss ratio of low value, the soluble solid of high value, titratable acid resist, ascorbic acid content represents that oranges and tangerines are fresher.
As can be seen here, the anti-mildew fresh-keeping of the biological composite mildewproof antistaling agent of oranges and tangerines of the present invention has extremely significant effect.
beneficial effect of the present invention
Above-mentioned result of the test shows, use the biological composite mildewproof antistaling agent of oranges and tangerines of the present invention to process oranges and tangerines, can significantly reduce the mould variability of oranges and tangerines in storage, can also improve peroxidase content, soluble solid content, titratable acid content and the ascorbic acid content of fruit simultaneously, and can reduce mda content and weight-loss ratio, and strengthen the resistance of fruit, keep the freshness of fruit, reduce the wastage, extend storage period.And avoided the harm of chemical preservative, improved edible security.
The specific embodiment
embodiment 1
Adopt following quality, parts by volume raw material to prepare the biological composite mildewproof antistaling agent of oranges and tangerines of the present invention: 1 part, glacial acetic acid, 2 parts of shitosans, bacillus amyloliquefaciens (
bacillus amyloliquefaciens) 1.5 parts of HRH.317 zymotic fluids, 1.5 parts of Tween-80s, 0.3 part of NaCl, 93 parts of distilled water.
The preparation method of bacillus amyloliquefaciens HRH.317 zymotic fluid is:
(1) prepare fluid nutrient medium and in 100 parts of distilled water, add 0.5 portion of beef extract, 1 part of peptone and 0.5 part of NaCl, regulate after pH to 7.2, sterilizing in 121 ℃, 20 minutes, makes nutrient solution;
(2) activation of bacillus amyloliquefaciens HRH317 is under aseptic condition, bacillus amyloliquefaciens HRH.317 is inoculated in the nutrient solution of (1) preparing with oese, under 37 ℃ of conditions, rotating speed is shaken cultivation 24 h in the constant-temperature table of 150 r/min, as seed liquor;
(3) seed liquor (2) having been activated, in the fluid nutrient medium of preparing with 1% (V/V) inoculum concentration access (1), at rotating speed, be shaken cultivation 24 h in the constant-temperature table of 150 r/min, obtain bacillus amyloliquefaciens HRH.317 basis zymotic fluid;
(4) in fluid nutrient medium prepared by the basic zymotic fluid access (1) of being prepared by (3), under 36-38 ℃, 160 r/min conditions, cultivate 20-24 h, expand to cultivate and obtain zymotic fluid, zymotic fluid viable count can reach 10
7-10
8cfu/mL.
The preparation method of the biological composite mildewproof antistaling agent of oranges and tangerines is: in 93 parts of distilled water, add the glacial acetic acid of 1 part (V/V) to make acetum; In acetum, add respectively the Tween-80 of the bacillus amyloliquefaciens HRH317 zymotic fluid of the shitosan of 2 parts (W/V), 1.5 parts (V/V), 1.5 parts (W/V) and the NaCl of 0.3 part (W/V), stir.
The using method of the biological composite mildewproof antistaling agent of oranges and tangerines comprises the steps:
(1) by without withered, not damaged, disease-free oranges and tangerines, after cleaning with running water, immerse the 1-2 min that sterilizes in 0.1% liquor natrii hypochloritis, the clorox of bearing fruit by sterile water wash after taking-up, and dry;
(2) oranges and tangerines after drying are immersed completely to biological composite mildewproof antistaling agent of the present invention, soak 30-40 second, pull out dry completely rear with thickness be 0.2-0.4 mm PE (polyethylene) freshness protection package (in advance on bag at interval of 15 cm places punchings, bore dia is 1 cm) packing, every bag of 25-40 fruit, stay 1/4 sack space, put under the condition that plastic crate is built in room temperature 18-23 ℃ and indoor relative humidity 70%-80% or be placed under reserve temperature 4-6 ℃, the condition of relative humidity 70%-80% and preserve.
embodiment 2
Adopt following quality, parts by volume raw material to prepare the biological composite mildewproof antistaling agent of oranges and tangerines of the present invention: 1 part, glacial acetic acid, 3 parts of shitosans, bacillus amyloliquefaciens (
bacillus amyloliquefaciens) 2 parts of HRH.317 zymotic fluids, 2 parts of Tween-80s, 0.5 part of NaCl, 93 parts of distilled water.
The preparation method of bacillus amyloliquefaciens HRH.317 zymotic fluid is with embodiment 1.
The preparation method of the biological composite mildewproof antistaling agent of oranges and tangerines is: in 93 parts of distilled water, add the glacial acetic acid of 1 part (V/V) to make acetum; In acetum, add respectively the Tween-80 of the bacillus amyloliquefaciens HRH317 zymotic fluid of the shitosan of 3 parts (W/V), 2 parts (V/V), 2 parts (W/V) and the NaCl of 0.5 part (W/V), stir.
The using method of the biological composite mildewproof antistaling agent of oranges and tangerines is with embodiment 1.
embodiment 3
Adopt following quality, parts by volume raw material to prepare the biological composite mildewproof antistaling agent of oranges and tangerines of the present invention: 1 part, glacial acetic acid, 2.5 parts of shitosans, bacillus amyloliquefaciens (
bacillus amyloliquefaciens) 1.8 parts of HRH.317 zymotic fluids, 1.7 parts of Tween-80s, 0.4 part of NaCl, 93 parts of distilled water.
The preparation method of bacillus amyloliquefaciens HRH.317 zymotic fluid is with embodiment 1.
The preparation method of the biological composite mildewproof antistaling agent of oranges and tangerines is: in 93 parts of distilled water, add the glacial acetic acid of 1 part (V/V) to make acetum; The bacillus amyloliquefaciens HRH317 zymotic fluid, the Tween-80 of 1.7 parts (W/V) and the NaCl of 0.4 part (W/V) that in acetum, add respectively the shitosan, 1.8 (V/V) of 2.5 parts (W/V), stir.
embodiment 4
Adopt following quality, parts by volume raw material to prepare the biological composite mildewproof antistaling agent of oranges and tangerines of the present invention: 1 part, glacial acetic acid, 2 parts of shitosans, bacillus amyloliquefaciens (
bacillus amyloliquefaciens) 1.5 parts of HRH.317 zymotic fluids, 1.5 parts of Tween-80s, 0.3 part of NaCl, 93 parts of distilled water.
The preparation method of bacillus amyloliquefaciens HRH.317 zymotic fluid is with embodiment 1.
The preparation method of the biological composite mildewproof antistaling agent of oranges and tangerines is: in 93 parts of distilled water, add the glacial acetic acid of 1 part (V/V) to make acetum; In acetum, add respectively the Tween-80 of the bacillus amyloliquefaciens HRH317 zymotic fluid of the shitosan of 2 parts (W/V), 1.5 parts (V/V), 1.5 parts (W/V) and the NaCl of 0.3 part (W/V), stir.
embodiment 5
Adopt following quality, parts by volume raw material to prepare the biological composite mildewproof antistaling agent of oranges and tangerines of the present invention: 1 part, glacial acetic acid, 3 parts of shitosans, bacillus amyloliquefaciens (
bacillus amyloliquefaciens) 2 parts of HRH.317 zymotic fluids, 2 parts of Tween-80s, 0.5 part of NaCl, 93 parts of distilled water.
The preparation method of bacillus amyloliquefaciens HRH.317 zymotic fluid is with embodiment 1.
The preparation method of the biological composite mildewproof antistaling agent of oranges and tangerines is: in 93 parts of distilled water, add the glacial acetic acid of 1 part (V/V) to make acetum; The bacillus amyloliquefaciens HRH317 zymotic fluid, the Tween-80 of 2 parts (W/V) and the NaCl of 0.5 part (W/V) that in acetum, add respectively the shitosan, 2 (V/V) of 3 parts (W/V), stir.
Claims (3)
1. the biological composite mildewproof antistaling agent of oranges and tangerines, is characterized in that being comprised of following quality, parts by volume raw material: 1 part, glacial acetic acid, and shitosan 2-3 part, bacillus amyloliquefaciens (
bacillus amyloliquefaciens) HRH.317 zymotic fluid 1.5-2 part, Tween-80 1.5-2 part, NaCl 0.3-0.5 part, 93 parts of distilled water;
The preparation method of the biological composite mildewproof antistaling agent of oranges and tangerines comprises the steps:
(1) in 93 parts of distilled water, add the glacial acetic acid of 1 part (V/V) to make acetum;
(2) in the acetum of preparing in (1), add respectively the shitosan of 2-3 part (W/V), the Tween-80 of the bacillus amyloliquefaciens HRH317 zymotic fluid of 1.5-2 part (V/V), 1.5-2 part (W/V) and the NaCl of 0.3-0.5 part (W/V) stir.
2. the biological composite mildewproof antistaling agent of oranges and tangerines according to claim 1, is characterized in that the preparation method of described bacillus amyloliquefaciens HRH.317 zymotic fluid comprises the steps:
(1) prepare fluid nutrient medium and in 100 parts of distilled water, add 0.5 portion of beef extract, 1 part of peptone and 0.5 part of NaCl, regulate after pH to 7.2, sterilizing in 121 ℃, 20 minutes, makes nutrient solution;
(2) activation of bacillus amyloliquefaciens HRH317 is under aseptic condition, bacillus amyloliquefaciens HRH.317 is inoculated in the nutrient solution of (1) preparing with oese, under 37 ℃ of conditions, rotating speed is shaken cultivation 24 h in the constant-temperature table of 150 r/min, as seed liquor;
(3) seed liquor (2) having been activated, in the fluid nutrient medium of preparing with 1% (V/V) inoculum concentration access (1), at rotating speed, be shaken cultivation 24 h in the constant-temperature table of 150 r/min, obtain bacillus amyloliquefaciens HRH.317 basis zymotic fluid;
(4) in fluid nutrient medium prepared by the basic zymotic fluid access (1) of being prepared by (3), under 36-38 ℃, 160 r/min conditions, cultivate 20-24 h, expand to cultivate and obtain zymotic fluid, zymotic fluid viable count is 10
7-10
8cfu/mL.
3. the biological composite mildewproof antistaling agent of oranges and tangerines according to claim 1, is characterized in that the using method of the biological composite mildewproof antistaling agent of oranges and tangerines is:
(1) by without withered, not damaged, disease-free oranges and tangerines, after cleaning with running water, immerse the 1-2 min that sterilizes in 0.1% liquor natrii hypochloritis, the clorox of bearing fruit by sterile water wash after taking-up, and dry;
(2) oranges and tangerines after drying are immersed completely to composite mildewproof antistaling agent of the present invention, soak 30-40 second, pull that to dry rear be completely the PE freshness protection package packing of 0.2-0.4 mm with thickness out, every bag of 25-40 fruit, stay 1/4 sack space, put under the condition that plastic crate is built in room temperature 18-23 ℃ and indoor relative humidity 70%-80% or be placed under reserve temperature 4-6 ℃, the condition of relative humidity 70%-80% and preserve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410242839.6A CN103999930B (en) | 2014-06-04 | 2014-06-04 | The biological composite mildewproof antistaling agent of a kind of oranges and tangerines and preparation and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410242839.6A CN103999930B (en) | 2014-06-04 | 2014-06-04 | The biological composite mildewproof antistaling agent of a kind of oranges and tangerines and preparation and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103999930A true CN103999930A (en) | 2014-08-27 |
| CN103999930B CN103999930B (en) | 2016-01-20 |
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Cited By (4)
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| CN104430834A (en) * | 2014-12-30 | 2015-03-25 | 华南师范大学 | Application of bacillus amyloliquefaciens strain B014 in postharvest preservation of fruits and vegetables |
| CN106615085A (en) * | 2016-12-19 | 2017-05-10 | 山西省农业科学院生物技术研究中心 | Microorganism and plant derived composite fresh keeping agent for fruits and vegetables |
| CN106720241A (en) * | 2016-11-17 | 2017-05-31 | 天津捷盛东辉保鲜科技有限公司 | Grape biology anti-mildew fresh-keeping method |
| CN113412858A (en) * | 2021-04-28 | 2021-09-21 | 四川大学 | Method for preserving blood orange by using bacillus amyloliquefaciens M73 fermentation product |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430834A (en) * | 2014-12-30 | 2015-03-25 | 华南师范大学 | Application of bacillus amyloliquefaciens strain B014 in postharvest preservation of fruits and vegetables |
| CN104430834B (en) * | 2014-12-30 | 2017-10-20 | 华南师范大学 | Applications of the Bacillus amyloliquefaciens strain B014 in postharvest fruit and vegetable is fresh-keeping |
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| CN106615085A (en) * | 2016-12-19 | 2017-05-10 | 山西省农业科学院生物技术研究中心 | Microorganism and plant derived composite fresh keeping agent for fruits and vegetables |
| CN106615085B (en) * | 2016-12-19 | 2020-01-10 | 山西省农业科学院生物技术研究中心 | Microbial and plant source composite fruit and vegetable fresh-keeping agent |
| CN113412858A (en) * | 2021-04-28 | 2021-09-21 | 四川大学 | Method for preserving blood orange by using bacillus amyloliquefaciens M73 fermentation product |
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| CN103999930B (en) | 2016-01-20 |
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