CN106171530A - A kind of method of imitative wildization cultivating black fungus - Google Patents

A kind of method of imitative wildization cultivating black fungus Download PDF

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Publication number
CN106171530A
CN106171530A CN201610663752.5A CN201610663752A CN106171530A CN 106171530 A CN106171530 A CN 106171530A CN 201610663752 A CN201610663752 A CN 201610663752A CN 106171530 A CN106171530 A CN 106171530A
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China
Prior art keywords
auricularia
weight portions
bag
culture medium
ear
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CN201610663752.5A
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Chinese (zh)
Inventor
陈喜君
王辉
王丽娜
杨国力
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Heilongjiang Zhongsheng Biological Engineering Co Ltd
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Heilongjiang Zhongsheng Biological Engineering Co Ltd
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Priority to CN201610663752.5A priority Critical patent/CN106171530A/en
Publication of CN106171530A publication Critical patent/CN106171530A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)

Abstract

A kind of method that the invention discloses imitative wildization cultivating black fungus.The present invention selects Northeast Forest Areas hardwood sawdust to be primary raw material cultivation strain, ecological condition needed for being grown by the wild Auricularia of simulation meets the requirement in Auricularia growth and development process to temperature, humidity, sunlight, air etc., make Auricularia ear shape, mouthfeel, thickness, color and luster all wild state Auricularia close to northeast, thus solve in artificial culture Auricularia growth course because the preventing and treating of planting type, cultivation technique and pest and disease damage does not passes a test, cause yielding poorly, and easy rotten ear, economic benefit is the highest, it is impossible to forms commercialization etc. and produces problem.

Description

A kind of method of imitative wildization cultivating black fungus
Technical field
The present invention relates to the cultural method of a kind of Auricularia, particularly to a kind of ring simulating northeast wild Auricularia growth The method of border cultivating black fungus.The invention belongs to cultivation of auricularia auricula technical field.
Background technology
In China Northeast Forest Areas, having Pinus koraiensis, broad-leaf forest is main Forest Types.In mountain forest, trees wood flour nutrient is rich Richness, Nutrient Cycling can be used, and grows and multiply abundant wild large-form fungi in excellent forest environment.
Auricularia is a kind of nutritious edible fungi, is again the traditional health food of China and exporting;It is because of life It is longer than on rotten wood, the ear of its likeness in form people, therefore named Auricularia.Domestic Auricularia market, is still that supply falls short of demand at present, and our province is current Auricularia mode of production Main Cultivation mode has the plantation of artificial juggle and artificial bag two kinds of methods of plantation.And juggle plantation needs Consuming the wood raw materials such as substantial amounts of basswood, birch, the amount fallen trees is increasing, causes ecological balance to destroy, soil erosion Deng, moreover the production cycle is longer, and the feature such as yield poorly;Artificial bag plantation formula operating technology cumbersome requirements is high, and the difficulty of grasp is big, And pollution rate is high, quality is low.Therefore for problem above, find and a kind of novel meet natural wild cultivation of auricularia auricula side Formula is the most necessary.Imitation biochemistry domestication wild Auricularia is a kind of domestication wild Auricularia that inventor draws after deliberation Novel planting type, is to simulate wild Auricularia and environmental condition, weather conditions, periphery plant growing relation, meets black wood Ear growth course is to requirements such as humidity, illumination, temperature, air, with fertile wood flour as raw material, and the beneficially one of Auricularia growth Pseudo-wild cultivating method, it is advantageous that natural environment grows, and pollution rate is low, and Auricularia yield is relatively high, and Auricularia meat Thickness, elastic big, there is no any pollution, for pure Organic food, there is good commercial value.
Summary of the invention
The technical problem to be solved is to provide a kind of method of imitative wildization cultivating black fungus, and the method is passed through Simulate wild Auricularia growing environment cultivating black fungus, solve a series of cultivations that artificial culture Auricularia growth course produces Problem.
In order to achieve the above object, present invention employs techniques below means:
The method of a kind of imitative wildization cultivating black fungus of the present invention, it is characterised in that comprise the following steps:
(1) prepared by culture medium:
By the hardwood sawdust of 85~90 weight portions, the Testa Tritici of 10~12 weight portions, the Gypsum Fibrosum powder of 1~2 weight portions, 1~2 The white lime use water mixing of the soybean cake of weight portion and 1~2 weight portions, adjusting pH value is 7~8, and water content is maintained at 60~65%, To the culture medium for domestication wild Auricularia;
(2) culture medium pack sterilizing:
Culture medium step (1) prepared loads Polypropylene Bag, and every bag 1~1.5kg, 100 DEG C of steam sterilizations 8 are little Time;
(3) inoculation:
Through the Bag Material of sterilizing, when material temperature drops to less than 30 DEG C, move on to inoculation Auricularia strain in transfer room or inoculating hood;
(4) mycelia culture:
It is 20~25 DEG C that postvaccinal cultivation bag is placed in temperature, and indoor humidity is 45%-~55%, carries out under conditions of lucifuge Cultivating, treat that mycelia is white, thick, stalwartness is advisable, incubation time is 12~15 days, and regulating the room temperature afterwards is 15~20 DEG C, suitably ventilate, Illumination, so that mycelium slowly adapts to external environment, treats that mycelia enters the maturation period, regulates the room temperature as 10-15 DEG C, humidity 75% ~85%, ventilate, illumination identical with external environment, culture period controlled at 55~65 days;
(5) scarfing:
Each cultivation bag cuts 10-22 V-type scarfing, deep 0.5cm, and length is 1.5~2.5cm;
(6) transplant:
Selecting light transmission is 30%~40%, and humidity is 75%~90%, well-ventilated, and ground is without hydrops and is coated with The broad-leaf forest of trees wood flour, moves on in broad-leaf forest by the thalline in period of maturation, the growth of pendulum ground;
(7) go out syrinx to manage:
Going out ear humid control 90%~95%, temperature, ventilation, sunlight are consistent with broad-leaf forest;
(8) ear is adopted:
When auricle is sufficiently spread out, the wrinkling thinning deliquescing in edge, color and luster turns light, and the basal part of the ear shrinks, and the outside of belly does not has white powder thing Time (spore powder), gather in time.
In method of the present invention, it is preferred that the preparation of the culture medium described in step (1) is by 85 weight portions Hardwood sawdust, the Testa Tritici of 12 weight portions, the Gypsum Fibrosum powder of 0.5 weight portion, the soybean cake of 2 weight portions and the white lime of 0.5 weight portion Mixing with water, adjusting pH value is 7.5, and controlling water content is 65%.
In method of the present invention, it is preferred that the hardwood sawdust forest zone northeast described in step (1) wealthy Leaf trees are considered to be worth doing.
In method of the present invention, it is preferred that the size of the Polypropylene Bag described in step (2) be 17cm × 33cm。
In method of the present invention, it is preferred that the month of the transplanting described in step (6) is annual August.
Compared to prior art, the present invention meets Auricularia by the ecological condition needed for simulating the growth of wild Auricularia Requirement to temperature, humidity, sunlight, air etc. in growth and development process, makes Auricularia ear shape, mouthfeel, thickness, color and luster the most close Northeast wild state Auricularia, thus reach that character is stable, resistance, quality is better than the purpose of other kinds.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further elaborated.The specific embodiment of description described herein is only Only in order to explain the present invention, it is not intended to limit the present invention.Technical solution of the present invention is not limited to the concrete real of act set forth below Execute mode, also include the combination in any between each detailed description of the invention.
The imitative wildization cultural method of embodiment 1 Auricularia
(1) prepared by culture medium:
By the hardwood sawdust of 85 weight portions, the Testa Tritici of 12 weight portions, the Gypsum Fibrosum powder of 0.5 weight portion, the soybean cake of 2 weight portions And 0.5 white lime of weight portion, mix with water, adjusting pH value is 7.5, and controlling water content is 65%, obtains for domestication wild black The culture medium of Auricularia;
(2) culture medium pack sterilizing:
Culture medium step (1) prepared loads the Polypropylene Bag of 17cm × 33cm, every bag of 1kg, steam sterilization 100 DEG C, sterilizing 8 hours;
(3) inoculation:
Through the Bag Material of sterilizing, when material temperature drops to less than 30 DEG C, in moving on to transfer room or inoculating hood, start inoculation;
(4) mycelia culture:
Postvaccinal cultivation bag is placed in temperature 25 DEG C, indoor humidity 50%, cultivates under conditions of lucifuge, treat mycelia white, Slightly, stalwartness be advisable, incubation time is 14 days, and regulating the room temperature afterwards is 20 DEG C, suitably ventilates, illumination, so that mycelium is slowly fitted Answering external environment, treat that mycelia enters the maturation period, regulating the room temperature is 15 DEG C, and humidity is 80%, ventilate, illumination and external environment phase With, culture period 60 days;
(5) scarfing:
Each cultivation bag cuts 15 V-type scarfings, and deep 0.5cm, length is at about 2cm;
(6) transplant:
Selecting light transmission is 30%~40%, and humidity is 75%~90%, well-ventilated, and ground is without hydrops and is coated with The broad-leaf forest of trees wood flour, moves on in broad-leaf forest by the thalline in period of maturation, the growth of pendulum ground;The month transplanted is August;
(7) go out syrinx to manage:
Going out ear humid control 90%~95%, temperature, ventilation, sunlight are consistent with broad-leaf forest;
(8) ear is adopted:
When auricle is sufficiently spread out, the wrinkling thinning deliquescing in edge, color and luster turns light, and the basal part of the ear shrinks, and the outside of belly does not has white powder thing Time (spore powder), gather in time.
The imitative wildization cultural method of embodiment 2 Auricularia
The present embodiment is as different from Example 1: by the hardwood sawdust of 90 weight portions, the wheat of 8 weight portions in step (1) Bran, the Gypsum Fibrosum powder of 0.5 weight portion, the soybean cake of 1 weight portion and the white lime of 0.5 weight portion, mix with water, adjusting pH value is 7, controls Water content is 60%, obtains the culture medium for domestication wild Auricularia.Other steps are same as in Example 1.
The imitative wildization cultural method of embodiment 3 Auricularia
The present embodiment is unlike embodiment 1 or embodiment 2: step (5) each cultivation bag cuts 18 big V-type scarfings, deeply 0.5cm, length is at about 2cm.Other steps are same as in Example 1.
The imitative wildization cultural method of embodiment 4 Auricularia
The present embodiment is as different from Example 1: postvaccinal cultivation bag is placed in step (4) temperature 20 DEG C, indoor wet Degree 55%, cultivates under conditions of lucifuge, treats that mycelia is white, thick, stalwartness is advisable, and incubation time is 12 days, regulates the room temperature afterwards Be 15 DEG C, suitably ventilate, illumination, so that mycelium slowly adapts to external environment, treat that mycelia enters the maturation period, regulate the room temperature into 10 DEG C, humidity is 75%, ventilate, illumination is identical with external environment, culture period 55 days.Other steps are same as in Example 1.
Comparative example
In order to further illustrate imitative wildization cultural method (carrying out according to method described in embodiment 1) and the tradition of the present invention The advantage of method (cultivation of artificial black fungus bag, artificial Auricularia juggle cultural method), the present invention uses three kinds of methods to enter simultaneously Having gone the experiment in cultivation of Auricularia, and the yield and character to Auricularia has carried out data analysis, result is as shown in table 1 below:
The yield of 1 three kinds of method plantation Auricularia of table and COMPARISON OF CHARACTERS
Analyze from upper table, implement the present invention and imitate wild cultivation of auricularia auricula and the cultivation of artificial black fungus bag, artificial black wood The cultivation of ear juggle is compared, and uses the inventive method to cultivate the Auricularia yield that obtains and cultivates apparently higher than juggle, and mouthfeel with Profile is close to wild Auricularia.Use the Auricularia yield height of the method cultivation of the pseudo-wild cultivating Auricularia of the present invention, quality The cultivation of the wild Auricularia in good, applicable northeast.
The foregoing is only presently preferred embodiments of the present invention, be merely illustrative for the purpose of the present invention, and non-limiting 's.All any amendment, equivalent and the improvement etc. made within the spirit and principles in the present invention of those skilled in the art, all Should comprise within the scope of the present invention.

Claims (5)

1. the method for an imitative wildization cultivating black fungus, it is characterised in that comprise the following steps:
(1) prepared by culture medium:
By the hardwood sawdust of 85~90 weight portions, the Testa Tritici of 10~12 weight portions, the Gypsum Fibrosum powder of 1~2 weight portions, 1~2 weight The soybean cake of part and the white lime use water mixing of 1~2 weight portions, adjusting pH value is 7~8, and water content is maintained at 60~65%, is used Culture medium in domestication wild Auricularia;
(2) culture medium pack sterilizing:
The culture medium that step (1) prepared loads Polypropylene Bag, every bag 1~1.5kg, 100 DEG C of steam sterilizations 8 hours;
(3) inoculation:
Through the Bag Material of sterilizing, when material temperature drops to less than 30 DEG C, move on to inoculation Auricularia strain in transfer room or inoculating hood;
(4) mycelia culture:
It is 20~25 DEG C that postvaccinal cultivation bag is placed in temperature, and indoor humidity is 45%-~55%, trains under conditions of lucifuge Supporting, treat that mycelia is white, thick, stalwartness is advisable, incubation time is 12~15 days, and regulating the room temperature afterwards is 15~20 DEG C, suitably ventilates, light According to so that mycelium slowly adapts to external environment, treat mycelia enter the maturation period, regulate the room temperature as 10-15 DEG C, humidity 75%~ 85%, ventilate, illumination identical with external environment, culture period controlled at 55~65 days;
(5) scarfing:
Each cultivation bag cuts 10-22 V-type scarfing, deep 0.5cm, and length is 1.5~2.5cm;
(6) transplant:
Selecting light transmission is 30%~40%, and humidity is 75%~90%, well-ventilated, and ground is without hydrops and is coated with trees The broad-leaf forest of wood flour, moves on in broad-leaf forest by the thalline in period of maturation, the growth of pendulum ground;
(7) go out syrinx to manage:
Going out ear humid control 90%~95%, temperature, ventilation, sunlight are consistent with broad-leaf forest;
(8) ear is adopted:
When auricle is sufficiently spread out, the wrinkling thinning deliquescing in edge, color and luster turns light, and the basal part of the ear shrinks, when the outside of belly does not has white powder thing, and Shi Caishou.
2. the method for claim 1, it is characterised in that the preparation of the culture medium described in step (1) is by 85 weight portions Hardwood sawdust, the Testa Tritici of 12 weight portions, the Gypsum Fibrosum powder of 0.5 weight portion, the soybean cake of 2 weight portions and 0.5 weight portion white Ash, mixes with water, and adjusting pH value is 7.5, and controlling water content is 65%.
3. the method for claim 1, it is characterised in that the hardwood sawdust forest zone northeast described in step (1) Hardwood sawdust.
4. the method for claim 1, it is characterised in that the size of the Polypropylene Bag described in step (2) be 17cm × 33cm。
5. the method for claim 1, it is characterised in that the month of the transplanting described in step (6) is annual August.
CN201610663752.5A 2016-08-12 2016-08-12 A kind of method of imitative wildization cultivating black fungus Pending CN106171530A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109220551A (en) * 2018-11-08 2019-01-18 黑龙江省农业科学院牡丹江分院 A kind of black fungus hemp stalk core culture medium and black fungus rapid culture method

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US4127965A (en) * 1976-11-08 1978-12-05 The Kinoko Company Method for growing wood mushrooms
CN101697693A (en) * 2008-11-20 2010-04-28 东北林业大学 Production technology for black fungus cultivation in simulated wild field
CN101953271A (en) * 2010-10-19 2011-01-26 深圳市东域投资发展有限公司 Imitation wild cultivation method of black fungus in jatropha curcas forest
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CN104350941A (en) * 2014-10-17 2015-02-18 广西靖西县壮佳农业发展有限责任公司 Method for cultivating funguses at bottoms of hawthorn trees by taking hawthorn tree branches and leaves as major raw materials
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JP2016116487A (en) * 2014-12-22 2016-06-30 独立行政法人国立高等専門学校機構 Auricularia auricula-judae cultivation method

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109220551A (en) * 2018-11-08 2019-01-18 黑龙江省农业科学院牡丹江分院 A kind of black fungus hemp stalk core culture medium and black fungus rapid culture method

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