CN106167758B - A kind of dispenser for DNA synthesizer - Google Patents
A kind of dispenser for DNA synthesizer Download PDFInfo
- Publication number
- CN106167758B CN106167758B CN201610741727.4A CN201610741727A CN106167758B CN 106167758 B CN106167758 B CN 106167758B CN 201610741727 A CN201610741727 A CN 201610741727A CN 106167758 B CN106167758 B CN 106167758B
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- CN
- China
- Prior art keywords
- valve
- inlet
- dna synthesizer
- plate
- mounting plate
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
Abstract
The invention discloses a kind of dispensers for DNA synthesizer, including valve plate mounting plate, multiple valve plates, multiple valves and multiple dispensing nozzles；Valve plate includes bar shaped plate body, bar shaped plate body includes two feed liquor portions and multiple liquid portions out, there are elongate holes in plate body, each liquid portion out includes the fluid hole that the inlet opening being connected to the elongate holes and one run through plate body upper and lower surface, liquid portion has groove out, the aperture of inlet opening and one of aperture of fluid hole are respectively positioned in the groove, and the feed liquor portion has the first inlet being connected to the elongate holes；Multiple valve plates are fixed on valve plate mounting plate, and the through hole that each fluid hole runs through valve plate mounting plate upper and lower surface with one is corresponded on valve plate mounting plate, and each through hole is also connected with one dispensing nozzle；The valve is fixed in each groove.Dispenser of the invention can carry out the operation of dispensing reagent to multiple synthesis columns simultaneously.
Description
Technical field
The present invention relates to field of biotechnology, and in particular to a kind of dispenser for DNA synthesizer.
Background technique
Artificial-synthetic DNA is the unique channel for the directional transformation gene order being currently known, and is widely used in protein and changes
It makes and the multiple fields such as life science, such as nucleic acid drug, enzyme engineering, gene therapy.DNA synthesizer needs de- in synthesis process
The different steps such as protection, activation link, nut cap, oxidation, synthesis process need to inject different reagents into synthesis column.Modern work
Industry needs to realize large-scale volume production, it is therefore desirable to high-throughput synthesizer, therefore the dispensing during high throughput synthesis also needs
Want special dispenser.
Summary of the invention
Goal of the invention: it the present invention is directed to overcome the deficiencies of existing technologies, provides a kind of suitable for high-throughput DNA synthesizer
Dispenser.
Technical solution: a kind of dispenser includes valve plate mounting plate, multiple valve plates, multiple valves and multiple dispensing nozzles；
The valve plate includes bar shaped plate body, and the bar shaped plate body includes positioned at two feed liquor portions at both ends and positioned at described two feed liquor portions
Between in an arrangement set it is multiple go out liquid portions, have in the plate body through two feed liquor portions and all strips for going out liquid portion
Shape hole, each going out liquid portion includes that the inlet opening being connected to the elongate holes and one run through the plate body upper and lower surface
Fluid hole, it is described go out liquid portion there is groove, one of aperture of the aperture of the inlet opening and the fluid hole is respectively positioned on institute
It states in groove, each feed liquor portion has the first inlet being connected to the elongate holes；The multiple valve plate is fixed on
On the valve plate mounting plate, each fluid hole is corresponded on the valve plate mounting plate with one through valve plate mounting plate upper and lower surface
Through hole, each through hole is also connected with one dispensing nozzle；The valve is fixed in each groove, it is described
The liquid feeding end of valve connects the inlet opening, and the outlet end of the valve connects the fluid hole.
Further, each feed liquor portion also has second inlet being connected to the elongate holes, described
Second inlet is cylindrical inlet, the axis of second inlet and the axis co-axial of the elongate holes.
Further, plug is equipped at two the second inlets of each valve plate, and two the first inlets are wherein
Plug is equipped at one inlet.
Further, the valve is two three-way electromagnetic valves.
Further, the dispensing nozzle and the through hole are threadably secured connection, and the dispensing nozzle is located at institute
It states below valve plate mounting plate.
Further, 10 valve plates are housed on the valve plate mounting plate.
Further, the bar shaped plate body includes 8 liquid portions out.
Further, the dispenser includes 80 dispensing nozzles.
The utility model has the advantages that dispenser of the invention can be realized while the dispensing operation of multiple synthesis columns, easy to use, energy
It is enough to realize high-throughput synthesis.
Detailed description of the invention
Fig. 1 is dispenser schematic diagram；
Fig. 2 is the first schematic diagram of valve plate；
Fig. 3 is the second schematic diagram of valve plate；
Fig. 4 is valve plate third schematic diagram；
Fig. 5 is the 4th schematic diagram of valve plate.
Specific embodiment
Appended drawing reference: 3 solenoid valves；4 dispensing nozzles；2 valve plate mounting plates；5 plate bodys；5.1 feed liquor portion；5.1.1 the first feed liquor
Mouthful；5.1.2 the second inlet；5.2 go out liquid portion；5.2.1 inlet opening；5.2.2 fluid hole；5.3 elongate holes.
A kind of dispenser includes valve plate mounting plate 2, multiple valve plates 5, multiple valves 3 and multiple dispensing nozzles 4；The valve
Plate 5 includes bar shaped plate body, and the bar shaped plate body includes positioned at two feed liquor portions 5.1 at both ends and positioned at described two feed liquor portions
Between 5.1 in an arrangement set it is multiple go out liquid portions 5.2, have in the plate body through two feed liquor portions 5.1 and all go out
The elongate holes 5.3 in liquid portion 5.2, each going out liquid portion 5.2 includes an inlet opening 5.2.1 being connected to the elongate holes 5.3
With a fluid hole 5.2.2 through the plate body upper and lower surface, the liquid portion 5.2 out has groove, the inlet opening 5.2.1
Aperture and one of aperture of the fluid hole 5.2.2 be respectively positioned in the groove, each feed liquor portion 5.1 have with
The first inlet 5.1.1 that the elongate holes 5.3 are connected to；The multiple valve plate 5 is fixed on the valve plate mounting plate 2, institute
It states and corresponds to the through hole that each fluid hole 5.2.2 runs through valve plate mounting plate upper and lower surface with one on valve plate mounting plate 2, each
Through hole is also connected with one dispensing nozzle 4；The valve 3, the feed liquor of the valve 3 are fixed in each groove
End connects the inlet opening, and the outlet end of the valve 3 connects the fluid hole.Each feed liquor portion 5.1 also has one
The second inlet 5.1.2 being connected to the elongate holes 5.3,5.1.2 mouthfuls of second feed liquor are cylindrical inlet, described
The axis co-axial of the axis of second inlet 5.1.2 and the elongate holes 5.3.Two the second inlets of each valve plate 2
5.1.2 place is equipped with plug, and plug is equipped at one of inlet of two the first inlet 5.1.1.The valve 3 is
Two three-way electromagnetic valves.The dispensing nozzle 4 and the through hole are threadably secured connection, and the dispensing nozzle 4 is located at the valve
2 lower section of plate mounting plate.10 valve plates are housed, the bar shaped plate body includes 8 and goes out liquid portion, the dispensing on the valve plate mounting plate
Device includes 80 dispensing nozzles.Other parameters also can be set into these certain parameters.
Dispenser of the invention, two the second inlets and first inlet of each valve plate are blocked by plug,
Only one first inlet connects inlet pipe.When fluid injection, liquid comes from inlet pipe, enters elongate holes from inlet
The inlet opening for entering each liquid portion out afterwards, flows into solenoid valve from inlet opening, comes out from solenoid valve and enter fluid hole, finally through excessive
Note nozzle enters in synthesis column.
Although the present invention is illustrated and has been described with regard to preferred embodiment, it is understood by those skilled in the art that
Without departing from scope defined by the claims of the present invention, variations and modifications can be carried out to the present invention.
Claims (8)
1. a kind of dispenser for DNA synthesizer, which is characterized in that including valve plate mounting plate, multiple valve plates, multiple valves
With multiple dispensing nozzles；The valve plate includes bar shaped plate body, and the bar shaped plate body includes two feed liquor portions and position positioned at both ends
Between described two feed liquor portions is in multiple liquid portions out that an arrangement is set, and is had in the plate body through two feed liquor portions and institute
Some goes out the elongate holes in liquid portion, and each going out liquid portion includes that the inlet opening being connected to the elongate holes and one run through institute
The fluid hole of plate body upper and lower surface is stated, the liquid portion out has groove, and the aperture of the inlet opening and the fluid hole are wherein
One aperture is respectively positioned in the groove, and each feed liquor portion has the first inlet being connected to the elongate holes；Institute
It states multiple valve plates to be fixed on the valve plate mounting plate, each fluid hole is corresponded on the valve plate mounting plate with one through valve
The through hole of plate mounting plate upper and lower surface, each through hole are also connected with a dispensing nozzle；One is fixed in each groove
A valve, the liquid feeding end of the valve connect the inlet opening, and the outlet end of the valve connects the fluid hole.
2. the dispenser according to claim 1 for DNA synthesizer, which is characterized in that each feed liquor portion is also
The second inlet being connected to one with the elongate holes, second inlet are cylindrical inlet, described second
The axis co-axial of the axis of inlet and the elongate holes.
3. the dispenser according to claim 2 for DNA synthesizer, which is characterized in that two of each valve plate
It is equipped with plug at two inlets, and is equipped with plug at one of inlet of two the first inlets.
4. the dispenser according to claim 1 for DNA synthesizer, which is characterized in that the valve is two energizations
Magnet valve.
5. the dispenser according to claim 1 for DNA synthesizer, which is characterized in that the dispensing nozzle and institute
It states through hole and is threadably secured connection, the dispensing nozzle is located at below the valve plate mounting plate.
6. the dispenser according to claim 1 for DNA synthesizer, which is characterized in that on the valve plate mounting plate
Equipped with 10 valve plates.
7. the dispenser according to claim 6 for DNA synthesizer, which is characterized in that the bar shaped plate body includes 8
A liquid portion out.
8. the dispenser according to claim 7 for DNA synthesizer, which is characterized in that the dispenser includes
80 dispensing nozzles.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610741727.4A CN106167758B (en) | 2016-08-26 | 2016-08-26 | A kind of dispenser for DNA synthesizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610741727.4A CN106167758B (en) | 2016-08-26 | 2016-08-26 | A kind of dispenser for DNA synthesizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106167758A CN106167758A (en) | 2016-11-30 |
| CN106167758B true CN106167758B (en) | 2018-12-11 |
Family
ID=57376895
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610741727.4A Active CN106167758B (en) | 2016-08-26 | 2016-08-26 | A kind of dispenser for DNA synthesizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106167758B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110272808B (en) | 2018-03-13 | 2020-10-16 | 武汉医蒂生物科技有限公司 | Nucleic acid extraction system |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1388333A (en) * | 2002-07-02 | 2003-01-01 | 瞿岳梦 | Branched flow divider |
| TW200801879A (en) * | 2006-06-15 | 2008-01-01 | Antera Biotech Corp | A uniform flow divider |
| CN100518840C (en) * | 2004-05-03 | 2009-07-29 | 金容年 | Device for regulating flow rate |
| TW201323754A (en) * | 2011-08-10 | 2013-06-16 | Fujikin Kk | Fluid control device |
| CN203447822U (en) * | 2013-08-20 | 2014-02-26 | 南京农业大学 | Chromatographic column suitable for 96-well tissue culture plate |
| CN103883865A (en) * | 2014-04-04 | 2014-06-25 | 周一平 | Quantified liquid supply device |
| CN205503434U (en) * | 2016-04-20 | 2016-08-24 | 四川帕沃可矿物纤维制品有限公司 | Activity chute with filter current drive system |
| CN205974497U (en) * | 2016-08-26 | 2017-02-22 | 江苏领坤生物科技有限公司 | A divide and annotate device for DNA synthesizer |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8697010B2 (en) * | 2007-12-19 | 2014-04-15 | Shimadzu Corporation | Dispensing device |
| JP6253547B2 (en) * | 2014-08-25 | 2017-12-27 | 株式会社日立製作所 | Liquid feeding device and chemical analyzer using the liquid feeding device |
-
2016
- 2016-08-26 CN CN201610741727.4A patent/CN106167758B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1388333A (en) * | 2002-07-02 | 2003-01-01 | 瞿岳梦 | Branched flow divider |
| CN100518840C (en) * | 2004-05-03 | 2009-07-29 | 金容年 | Device for regulating flow rate |
| TW200801879A (en) * | 2006-06-15 | 2008-01-01 | Antera Biotech Corp | A uniform flow divider |
| TW201323754A (en) * | 2011-08-10 | 2013-06-16 | Fujikin Kk | Fluid control device |
| CN203447822U (en) * | 2013-08-20 | 2014-02-26 | 南京农业大学 | Chromatographic column suitable for 96-well tissue culture plate |
| CN103883865A (en) * | 2014-04-04 | 2014-06-25 | 周一平 | Quantified liquid supply device |
| CN205503434U (en) * | 2016-04-20 | 2016-08-24 | 四川帕沃可矿物纤维制品有限公司 | Activity chute with filter current drive system |
| CN205974497U (en) * | 2016-08-26 | 2017-02-22 | 江苏领坤生物科技有限公司 | A divide and annotate device for DNA synthesizer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106167758A (en) | 2016-11-30 |
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