CN106138478A - The medicine of a kind of enhancing immunity or health food - Google Patents
The medicine of a kind of enhancing immunity or health food Download PDFInfo
- Publication number
- CN106138478A CN106138478A CN201510131943.2A CN201510131943A CN106138478A CN 106138478 A CN106138478 A CN 106138478A CN 201510131943 A CN201510131943 A CN 201510131943A CN 106138478 A CN106138478 A CN 106138478A
- Authority
- CN
- China
- Prior art keywords
- medicine
- enhancing immunity
- vitamin
- health food
- soybean isoflavone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to medicine or the health food of a kind of enhancing immunity.It is characterized in that containing functional component: soybean isoflavone (daiazi, daidzein, genistein, genistin), procyanidin, vitamin E.Pharmacological research shows that it has good enhancing immunity effect.
Description
Technical field
The present invention relates to field of medicaments, be specifically related to a kind of medicine with enhancing immunity effect or health food.
Background technology
Women is at society in occupation of consequence, and operating pressure, the life stress born are increasing, especially to middle-aged women, face the series of problems such as climacteric, and the pressure born is bigger.This just makes us in the face of an important problem: the most safely and effectively enhancing immunity, reduces the harm that fracture, spinal curvature etc. are brought, and safeguards the health of middle aged and aged women, improves quality of life and quality of life.
Summary of the invention
It is an object of the invention to provide medicine or the health food of a kind of safely and effectively enhancing immunity.
The medicine of enhancing immunity of the present invention or health food, mainly contain following raw material: soybean isoflavone, Semen Vitis viniferae extract, vitamin E.
The medicine of enhancing immunity of the present invention or health food, mainly contain following functional component: soybean isoflavone (daiazi, daidzein, genistein, genistin), procyanidin, vitamin E.
Wherein, during soybean isoflavone is Semen sojae atricolor rich in natural active matter.It is that a class has extensive threpsology value, healthy protection and the non-steroid substance of therapeutic consequences.Internal and external test shows that edible isoflavone has important function to the preventions such as osteoporosis, menopausal women syndrome, tumor, cardiovascular disease and regulation, is a kind of active substance with fine health-care effect.Due to soybean isoflavone estrogen-like effect in vivo, antioxidation, and the regulation effect of multiple enzyme is played health value widely.
Procyanidin is Semen Vitis viniferae extract, the effective ingredient of Pericarpium Vitis viniferae extract in Angiospermae, Dicotyledoneae, Fructus Vitis viniferae mesh, Vitaceae, Vitis, Fructus Vitis viniferae, it it is the most potent free radical scavenger found so far, having procyanidin (Semen Vitis viniferae extract) is a kind of new and effective antioxidant, has the strongest activity in vivo.It is demonstrated experimentally that the free radical resisting oxidability of OPC is 50 times of vitamin E, ascorbic 20 times, and absorb the most completely, and it being administered orally and i.e. can reach the highest haemoconcentration in 20 minutes, metabolic half life is for 7 hours.
Vitamin E is a kind of fatsoluble vitamin, and its hydrolyzate is tocopherol, is one of topmost antioxidant.Tocopherol can promote sex hormones secretion, makes woman's estrogen concentration increase, and alleviates climacteric syndrome etc..
The present invention selects soybean isoflavone estrogen-like effect, procyanidin antioxidation, and vitamin E promotes sex hormones secretion.All medicines share, multinomial compatibility common enhancing human body immunity power.Fundamentally strengthen the immunity of middle aged and aged women.
This medicine or the optimised process of health food:
Weigh the raw material of following weight proportion: soybean isoflavone 4.228 parts, Semen Vitis viniferae extract 4.88 parts, vitamin E 0.468 part, add starch 10.284 weight portion, silicon dioxide 0.1 weight portion, magnesium stearate 0.04 weight portion, mixing, capsule fill, make capsule.
This medicine or health food have good enhancing immunity effect.
Relevant pharmacological experimental data
1, laboratory animal
Experiment cleaning grade female ICR white mice, body weight (20 ± 2) g.Detection environmental condition, temperature range 20 ~ 25 DEG C, relative humidity 40% ~ 70%.In Animal House environment, 3d is adapted to before mouse experiment.
2, test method
2.1, experiment packet and cycle each pilot project all set up experimental group and the negative control group of various dose, often group 10, wherein experimental group dosage is respectively by human body recommended dose 1.6g/d(60kg*d) 5 times, 10 times, 30 times of designs low dose group (0.13g/kg), middle dosage group (0.27g/kg), high dose group (0.80g/kg) all experimental grouies per os (gavage) give the sample of various dose, gavage volume is 0.1ml/10g body weight, once/d, counts 30d；Negative group gives the normal saline with same volume.
2.2, experimental technique and interpretation of result
2.2.1, splenocyte Cell transformation test: experiment the 30th day, every mice is aseptic took spleen, is placed in and fills in appropriate aseptic Hanks liquid plate, is ground by spleen gently with tweezers, make single cell suspension.Filter through 200 eye mesh screens, or with 4 layers of gauze, spleen is ground, wash 2 times with Hanks liquid, be centrifuged 10min(1000r/min every time).Then, by cell suspension in 1ml complete culture solution, with platform phenol indigo plant dyeing counting viable count (should be more than 95%), adjusting cell concentration is 3 × 106/ml.Cell suspension point holes being added in 24 well culture plates, every hole 1ml, a hole adds 75 μ l ConA liquid (being equivalent to 7.5 μ g/ml), and 5% CO2, as comparison, is put in another hole, cultivates 72h in 37 DEG C of CO2 incubators.Cultivation terminates front 4h, and every hole sucks supernatant 0.7ml gently, adds the 0.7ml RPMI1640 culture fluid without calf serum, is simultaneously introduced MTT(5mg/ml) 50 μ l/ holes, continue to cultivate 4h.After cultivation terminates, every hole adds 1ml acid isopropyl alcohol, piping and druming mixing, makes purple crystal be completely dissolved.Then solution is moved in cuvette, measure optical density value OD value with 721 spectrophotometers at wavelength 570nm.Finally deduct the optical density value in ConA hole by the optical density value adding ConA hole and represent the multiplication capacity of lymphocyte.
2.2.2, mice serum hemolysin test: at experiment the 25th day, every Mus abdomen injection 0.2ml2%(V/V) hematocrit sheep red blood cell (SRBC) (SRBC) suspension, carry out immunity.After 5d, take blood and be centrifuged, collect serum, with normal saline by serum doubling dilution, 37 DEG C of incubation 3h, observe hemagglutination degree, calculating antibody product.
2.2.3 30min before, macrophage phagocytic chicken red blood cell experimental animal is put to death, every Mus abdomen injection 1.0ml20%(V/V) chicken erythrocyte suspension, place after death reinjects 2ml normal saline, take abdominal cavity drop sheet, 37 DEG C of incubation 30min, fix, dyeing, microscopy, counts 100 macrophages, calculates phagocytic rate and phagocytic index.
2.2.4, internal organs/body weight ratio put to death animal, take its thymus and spleen, weigh, calculate thymus/body weight and spleen/body weight ratio.
2.2.5, the multiple samples of experimental data statistics compare employing variance analysis process data；Multisample compares employing q inspection two-by-two.P ＜ 0.05 has statistical significance for difference.
3, result
3.1, the impact of the mice spleen lymphocytes proliferation ability that ConA is induced by the lymphopoiesis ability present invention, is shown in Table 1.Experimental group compares with negative control group, and three dosage groups all can significantly improve the multiplication capacity of splenocyte.
The impact (g/kg) of the mice spleen lymphocytes proliferation ability that ConA is induced by table 1 present invention
Compared with negative control group, P < 0.05
The 3.2 serum hemolysin present invention impact on mice serum hemolysin, is shown in Table 2.Experimental group compares with negative control group, and three dosage groups all can significantly improve mice serum hemolytic antibody product level.
Table 2 present invention impact (g/kg) on the impact of mice serum hemolysin
Compared with negative control group, P < 0.05
3.3, the peritoneal macrophage phagocytosis chicken red blood cell present invention impact on Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell, is shown in Table 3.Experimental group compares with negative control group, and three dosage groups all can improve the phagocytic rate of spleen Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell.
Table 3 present invention impact [g/kg(%)] on Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell
| Group | Dosage | Number of animals | Phagocytic rate | Anti-volumetric quantities |
| Negative control group | —— | 10 | 3.65±4.1 | 0.92±0.33 |
| 0.13 | 10 | 44.7±5.5 | 1.07±0.56 | |
| Experimental group of the present invention | 0.27 | 10 | 45.5±5.1 | 1.14±0.12 |
| 0.80 | 10 | 44.5±4.8 | 0.91±0.47 | |
| F value | —— | —— | 3.301 | 0.432 |
| P value | —— | —— | 0.021 | 0.041 |
Compared with negative control group, P < 0.05
3.4, internal organs/proportion ratio present invention impact on mice organs/body weight ratio, is shown in Table 4.Three dosage group mouse thymus/body weight ratios and spleen/body weight ratio and negative control group compare, and there was no significant difference (P ＞ 0.05)
Table 4 present invention impact (g/kg, mg/g) on mice organs/proportion ratio
| Group | Dosage | Number of animals | Thymus/body weight | Spleen/body weight |
| Negative control group | —— | 10 | 2.4±0.4 | 4.8±1.0 |
| 0.13 | 10 | 2.4±0.3 | 4.4±0.4 | |
| Experimental group of the present invention | 0.27 | 10 | 2.5±0.2 | 4.6±0.1 |
| 0.80 | 10 | 2.5±0.1 | 4.6±0.2 | |
| F value | —— | —— | 0.321 | 1.515 |
| P value | —— | —— | 0.604 | 0.027 |
Compared with negative control group, P ＞ 0.05
1, discuss
According to enhancing immunity experimental result decision rule in " function of health food assessment process and the method for inspection ": " enhancing immunity function judges: in cellular immune function, humoral immune function, mononuclear phagocyte function, any two aspect results positives of four aspects of NK cytoactive, can determine that this given the test agent has enhancing immunity function." the study show that: the present invention is on mouse thymus/body weight ratio and spleen/body weight ratio without impact, and the present invention can improve the multiplication capacity of the mouse spleen lymphocyte of ConA induction, can improve mice serum hemolytic antibody product level；The phagocytic rate of Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell can be improved, illustrate that the present invention has the effect of enhancing immunity.
Detailed description of the invention
Embodiment
1
:
Weigh soybean isoflavone 4.228 parts, Semen Vitis viniferae extract 4.88 parts, vitamin E 0.468 part, add starch 10.284 weight portion, silicon dioxide 0.1 weight portion, magnesium stearate 0.04 weight portion, mixing, capsule fill, make capsule.
Embodiment
2
:
Weigh soybean isoflavone 4.5 parts, Semen Vitis viniferae extract 4 parts, vitamin E 0.5 part add magnesium stearate 0.05 weight portion, starch 6 weight portion, microcrystalline Cellulose 1.5 weight portion, mixing, and capsule fill is packaged into bottle.
Embodiment
3
:
Weigh soybean isoflavone 6 parts, Semen Vitis viniferae extract 4 parts, vitamin E 3 parts add magnesium stearate 0.25 weight portion, starch 6 weight portion, microcrystalline Cellulose 1.5 weight portion, and mixing is pelletized, and 75 DEG C are dried, and granulate is packaged into bag.
Embodiment
4
:
Weigh soybean isoflavone 10 parts, Semen Vitis viniferae extract 1 part, vitamin E 1 part add magnesium stearate 0.25 weight portion, starch 7 weight portion, silicon dioxide 0.2 weight portion, and mixing is pelletized, and 75 DEG C are dried, and granulate is packaged into bag.
Embodiment
5
:
Weigh soybean isoflavone 6 parts, Semen Vitis viniferae extract 4 parts, vitamin E 3 parts add magnesium stearate 0.25 weight portion, starch 4 weight portion, microcrystalline Cellulose 2 weight portion, mixing, and tabletting is aluminum-plastic packaged, is packaged into box.
Embodiment
6
:
Weigh soybean isoflavone 4 parts, Semen Vitis viniferae extract 4 parts, vitamin E 3 parts add magnesium stearate 0.25 weight portion, starch 5 weight portion, and mixing is pelletized, and 75 DEG C are dried, granulate, and capsule fill is aluminum-plastic packaged, is packaged into box.
Claims (6)
1. a medicine with enhancing immunity function or health food, it is characterised in that containing active ingredient: soybean isoflavone (daiazi, daidzein, genistein, genistin), procyanidin, vitamin E.
The medicine with enhancing immunity function the most according to claim 1 or health food, it is characterised in that optimal active ingredient contained by it: soybean isoflavone, Semen Vitis viniferae extract, vitamin E.
The medicine with enhancing immunity function the most according to claim 1 or health food, it is characterised in that its weight proportion containing each composition is: soybean isoflavone 1 ~ 40 part, procyanidin 1 ~ 40 part, vitamin E
0.1 ~ 10 part.
The medicine with enhancing immunity function the most according to claim 3 or health food, it is characterised in that its weight proportion containing each composition is: soybean isoflavone 1 ~ 40 part, Semen Vitis viniferae extract 1 ~ 40 part, vitamin E 0.1 ~ 10 part.
The medicine with enhancing immunity function the most according to claim 4 or health food, it is characterised in that its optimum weight proportioning containing each composition is: soybean isoflavone 4.228 parts, Semen Vitis viniferae extract 4.88 parts, vitamin E 0.468 part.
The medicine with enhancing immunity function the most according to claim 5 or health food, it is characterized in that its preparation technology is: weigh the soybean isoflavone of above-mentioned weight proportion, Semen Vitis viniferae extract, vitamin E add magnesium stearate 0.04 weight portion, starch 10.284 weight portion, silicon dioxide 0.1 weight portion, mixing, capsule fill, makes capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510131943.2A CN106138478A (en) | 2015-03-25 | 2015-03-25 | The medicine of a kind of enhancing immunity or health food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510131943.2A CN106138478A (en) | 2015-03-25 | 2015-03-25 | The medicine of a kind of enhancing immunity or health food |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106138478A true CN106138478A (en) | 2016-11-23 |
Family
ID=57340359
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510131943.2A Pending CN106138478A (en) | 2015-03-25 | 2015-03-25 | The medicine of a kind of enhancing immunity or health food |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106138478A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1618333A (en) * | 2004-12-09 | 2005-05-25 | 桂林莱茵生物制品有限公司 | Anti-oxidation health-care food, and its prodn. method |
-
2015
- 2015-03-25 CN CN201510131943.2A patent/CN106138478A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1618333A (en) * | 2004-12-09 | 2005-05-25 | 桂林莱茵生物制品有限公司 | Anti-oxidation health-care food, and its prodn. method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101244110B (en) | Preparation method of Chinese medicine composition with function of releasing fatigue, reinforcing immunity | |
| CN103800661B (en) | A kind of enhancing immunity, alleviating physical fatigue compositions and containing its preparation | |
| CN105852102A (en) | Dendrobium composite chewable tablet and preparation method thereof | |
| CN106729136A (en) | A kind of functional food for adjusting immunity | |
| CN105394749B (en) | A kind of Ricipe for health care food and preparation method and application containing astaxanthin | |
| CN107184897A (en) | Composition with invigorating Qi and tonifying kidney effect and preparation method thereof | |
| CN104799286B (en) | Composition for improving immunity and preparation method of composition | |
| CN103169737A (en) | Composite of Antrodia camphorata entity and shell-broken ganoderma lucidum spore powder and application thereof in immune adjustment | |
| CN108497498A (en) | A kind of functional food of relieving alcoholism and protecting liver and preparation method thereof | |
| CN105055855B (en) | With Chinese medicine composition for improving sleep, strengthen immunity effect and its preparation method and application | |
| CN104758556B (en) | A kind of compound preparation and preparation method thereof of anti-Heat stress | |
| CN108514114A (en) | Composition for relieving physical fatigue and purposes based on chondriosome nutrient theory | |
| CN110680902A (en) | Traditional Chinese medicine composition for treating laying hen fatty liver | |
| CN104546822B (en) | The medical usage of epimedium aglucone | |
| CN106389477B (en) | A kind of preparation method and application of the full cellular plant oil extract of Gordonia terrae | |
| CN106138478A (en) | The medicine of a kind of enhancing immunity or health food | |
| CN105639620A (en) | Health food composition for increasing bone mineral density, and preparation method thereof | |
| CN105560308B (en) | Flower of JINHUAKUI is preparing the application in the product for preventing and treating prostatic disorders | |
| CN104288164A (en) | Pharmaceutical composition for preventing acute alcoholic liver injury | |
| CN103829254A (en) | Application of proanthocyanidins in preparing diet food or drug | |
| CN108057049A (en) | A kind of Chinese medicine composition for enhancing cell viability and preparation method thereof | |
| CN107114790A (en) | A kind of health food of strengthen immunity and preparation method thereof | |
| CN106880784A (en) | It is a kind of with fatigue-relieving, the Chinese medicine composition of anti-aging and its application | |
| CN103041368B (en) | Application of phycocyanin in preparation of drug for preventing and treating embryotoxicity caused by organophosphorus pesticide | |
| CN103784492A (en) | Pratia begonafolia (Wall) Lindl. or P. nummularia (Lam.)A.Br.et Aschers extract with antitumor effect and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20161123 |