CN106124697A - The appraisal procedure of a kind of plant regulation of absorbing dust capability and application thereof - Google Patents
The appraisal procedure of a kind of plant regulation of absorbing dust capability and application thereof Download PDFInfo
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- CN106124697A CN106124697A CN201610417089.0A CN201610417089A CN106124697A CN 106124697 A CN106124697 A CN 106124697A CN 201610417089 A CN201610417089 A CN 201610417089A CN 106124697 A CN106124697 A CN 106124697A
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Abstract
The invention discloses appraisal procedure and the application thereof of a kind of plant regulation of absorbing dust capability.Use electron-microscope scanning, observe blade micro structure, including the stomatal frequency of blade, and there is the situation of cilium；Meanwhile, after abundant photoinduction, plant leaf blade Photosynthetic parameter Cond stomatal conductance is measured；Stomatal conductance (Cond) in the micro structure on foundation plant leaf blade surface and Photosynthetic parameter, is estimated plant regulation of absorbing dust capability.Blade lower epidermis has cilium, and stomatal frequency is higher, and the plant regulation of absorbing dust capability that Cond value is bigger is stronger；The plant regulation of absorbing dust capability that blade lower epidermis is smooth, stomatal frequency is relatively low, Cond value is less is more weak.The plant regulation of absorbing dust capability appraisal procedure that the application present invention provides, can determine plant regulation of absorbing dust capability convenient, effectively, provides scientific basis for carrying out the reasonability of plant arrangement in city, afforestation.
Description
Technical field
The present invention relates to a kind of appraisal procedure to plant regulation of absorbing dust capability and application thereof.
Background technology
Along with global industry and the fast development of urbanization, Particulate Pollution has become as serious urban environment and asks
Topic.Ornamental plant, as natural air purifier, has preferable regulation of absorbing dust capability, can be effectively improved by its effect of laying the dust
Air quality.
At present, the regulation of absorbing dust capability result of study of plant shows, regulation of absorbing dust capability between seeds exists the reason master of larger difference
Relevant with plant leaf blade surface roughness, petiole length and Leaf insertion angle；The difference of the different plant regulation of absorbing dust capability of research
Different, it is considered the main and surface characteristic (wrinkle, coarse, fine hair, oils and fats etc.) of blade and wettability has substantial connection, stagnant
Dust quantity number relevant with the total leaf area of tree crown, branch crotch angle, shape of tree-crown etc. (see document: " ecological science ",
2006,25 (5): 395-399).In terms of the method being estimated plant regulation of absorbing dust capability, document reports what employing was correlated with
Index and evaluation analysis method, set up correlation model, is analyzed the relevant factor affecting plant leaf blade regulation of absorbing dust capability simultaneously；
Employing difference weight method has carried out laying the dust measuring and has determined, and studies the impact on regulation of absorbing dust capability of the blade face characteristic, and uses composite index law to it
Regulation of absorbing dust capability has carried out assessing (seeing document: " Shandong forestry science and technology ", 2015,45 (1): 22-25)；Document there was reported profit
(document is seen: " should by the relation of the leaf epidermis morphology structure of the more multiple ornamental plant of ESEM with the amount of laying the dust
With Acta Ecologica Sinica ", 2002,13 (9): 1121-1126).But prior art only from the observation of plant leaf blade surface texture, compare
Judge plant regulation of absorbing dust capability, there is certain one-sidedness.By observing blade surface micro structure in combination with plant photosynthesis
Physiological parameter, the method being estimated plant regulation of absorbing dust capability have not been reported.
Summary of the invention
The present invention is directed to the deficiency that prior art exists, it is provided that one is from observation of plant blade surface micro structure and measures light
The method that plant regulation of absorbing dust capability is estimated by conjunction physiological parameter aspect, and it is applied to the Tree Species Selection of gardens or urban afforestation,
Have comprehensive and scientific.
The technical scheme realizing the object of the invention is to provide the appraisal procedure of a kind of plant regulation of absorbing dust capability, including walking as follows
Rapid:
(1) select fully deployed plant leaf blade, the lower epidermis of blade is used electron-microscope scanning, observes blade micro structure, including
The stomatal frequency of blade, and there is the situation of cilium；
(2) choose that the upgrowth situation observed with step (1) be similar, the consistent plant leaf blade of leaf age and leaf position, through fully
After photoinduction, plant leaf blade Photosynthetic parameter Cond stomatal conductance is measured；
(3) according to step (1) and the result of (2), plant regulation of absorbing dust capability is estimated；Blade lower epidermis has planting of cilium
Thing, its regulation of absorbing dust capability strengthens along with the increase of stomatal frequency value.
The appraisal procedure of plant regulation of absorbing dust capability of the present invention, when Cond stomatal conductance value is less than 0.05 mol H2O m-2·s-1Time, plant regulation of absorbing dust capability is weak；Cond stomatal conductance value is at 0.05～0.10 mol H2O m-2s-1Time, in regulation of absorbing dust capability
Deng；Cond stomatal conductance value is more than 0.1 mol H2O m-2·s-1Time, regulation of absorbing dust capability is strong.
Technical solution of the present invention also includes being applied to city, gardens of the appraisal procedure by described plant regulation of absorbing dust capability
The plant arrangement of greening.
Plant regulation of absorbing dust capability is made the principle of assessment institute foundation by the present invention: the pore of plant is mainly distributed on blade
Lower epidermis, the shape of different plant leaf blade lower surfaces, the density of pore and stretching degree the most difference.Gas when plant leaf blade
Hole density is higher, and stomatal opening degree is relatively big, and when lower epidermis has cilium, blade regulation of absorbing dust capability is higher；Stomatal frequency is relatively low, gas
Hole stretching degree is less, and lower epidermis is smooth, and when having less or fibre-less, blade regulation of absorbing dust capability is more weak.The stretching degree of pore
Being stomatal conductance (Cond), the present invention is by the statistical to the different plant leaf blade unit are amounts of laying the dust with blade Cond value
Analysis shows, the Cond value of blade is the biggest, and its regulation of absorbing dust capability is the strongest, and both correlation coefficienies reach 0.549, in extremely notable positive
Close.Therefore, the regulation of absorbing dust capability of this plant can be made science, effectively according to the surface texture of plant leaf blade and stomatal conductance thereof
Assessment.
Compared with prior art, the present invention carries out Scientific evaluation to the regulation of absorbing dust capability of plant in terms of different effectively,
And method simple and fast；It is applied to the Greening Design in gardens, city, can be the different requirements to green tree species of the different regions, city
Suitable seeds are selected to provide scientific basis.
Accompanying drawing explanation
Fig. 1 is the electron-microscope scanning figure of the Radix Cinnamomi porrecti Flos Carthami wood blade lower surface that the embodiment of the present invention provides.
Detailed description of the invention
With embodiment, technical solution of the present invention is further described below in conjunction with the accompanying drawings.
Embodiment 1
The present embodiment technical scheme is applicable to the ornamental and afforestation trees such as common arbor, shrub, by blade surface fine structure
Observing and the mensuration of Photosynthetic parameter, be estimated plant leaf blade regulation of absorbing dust capability, it specifically comprises the following steps that
1, the electron-microscope scanning of leaf surface fine structure
From tree body, select appropriate blade, be sealed in the interior anti-extrusion simultaneously of sample sack immediately or blade cilium is destroyed, take back reality
Test indoor, along the middle part of vein both sides, fresh blade is cut into the blockage of the length of side about 5mm, is immediately placed in 2.5% glutaraldehyde solution
In, put in 4 DEG C of refrigerators and fix 4h；
Rinse 2 times with the phosphate buffer of 0.1mol, each 30min；
Graded ethanol be dehydrated, use 30%(2 time, 10min), 50%(2 time, 10min), 70%(1 time, soaked overnight), 90%(1 time,
15min) and 100%(1 time, 15min) 5 gradients；
Replace 2 times in isoamyl acetate after dehydration, each 15min；
Vacuum drying, takes out viscous platform after having ice crystal volatilization in bottle；
Metal spraying, in the upper observation of scanning electron microscope (S-570, FDAC).
2, the mensuration of Photosynthetic parameter-Cond
Choose vegetation growth state to be similar to, the consistent blade of leaf age and leaf position, after abundant photoinduction, utilize portable photosynthetic
The effect analyzer Li-6400XT(U.S., Li-COR company) measure the Cond value of different plant, use red blue-light source during mensuration,
Sample room flow rate set is 500 μm ol s-1, intensity of illumination is set as 1500 μm ol m-2s-1, Atmospheric CO2 Concentration controls
At 400 μm ol mol-1Left and right.
3, the mensuration of the unit leaf area amount of laying the dust
Behind 7 days that rain stops, gather each 30～50 of the blade at upper, middle and lower position around tree and shrubs, put in freshness protection package, weight
Second mining sample 3 times, after distilled water immersion two hours, and brushes off the attachment of residual on blade with banister brush, then will with tweezers
Blade carefully presss from both sides out, clean with distilled water flushing, embathes liquid with drying the filter paper sucking filtration to constant weight (W1), by filter paper in 80 DEG C
Lower drying, to constant weight, is re-weighed (W2), and the difference of twice weight is and gathers depositing dust particulate matter weight accompanying on sample.
The blade gone out by folder is put in freshness protection package, utilizes EPSONPERFECTION V700 PHOTO root scanner analysis
Going out out its leaf area is S.The unit are amount of laying the dust of plant leaf blade is (W2-W1)/S.
4, the unit leaf area amount of laying the dust determines with leaf surface structure and Cond value relation thereof
It is right that mensuration according to the different plant units leaf area amounts of laying the dust and its blade lower epidermis electron microscope scanning result are carried out
Ratio is visible: blade lower epidermis has cilium, and the plant amount of laying the dust that stomatal frequency is higher is relatively big, and blade lower epidermis is smooth, pore is close
Spend the relatively low plant amount of laying the dust less；By the statistical analysis to the different plant leaf blade unit are amounts of laying the dust with blade Cond value
Show: the Cond value of plant leaf blade and the blade amount of laying the dust are in extremely notable positive correlation, and the Cond value of blade is the biggest, and regulation of absorbing dust capability is more
By force, correlation coefficient between the two reaches 0.549；Plant can be divided three classes according to the size of Cond value accordingly: Cond value is less than
0.05 mol H2O m-2· s-1Plant divide the first kind, regulation of absorbing dust capability is more weak；Cond is 0.05～0.10 mol H2O m-2·
s-1For Equations of The Second Kind, regulation of absorbing dust capability is moderate；Cond is more than 0.1 mol H2O m-2· s-1For the 3rd class, regulation of absorbing dust capability is relatively
By force.
5, the size of plant regulation of absorbing dust capability is assessed according to leaf surface fine structure and blade Cond value
Can be determined that with leaf surface structure and Cond value relation thereof according to the unit leaf area amount of laying the dust, blade lower epidermis is smooth,
Less or fibre-less, stomatal frequency is relatively low, and Cond value is less than 0.05 mol H2O m-2· s-1Plant regulation of absorbing dust capability more weak；Leaf
Sheet lower epidermis has cilium, and stomatal frequency is higher, and Cond value is more than 0.10 mol H2O m-2· s-1Plant regulation of absorbing dust capability relatively
By force.
6, suitable Landscape Trees is selected according to plant regulation of absorbing dust capability size
By to leaf surface Microstructure observation and the mensuration of Cond value, determine the regulation of absorbing dust capability size of plant, thus select to close
Suitable Landscape Trees carries out rational plants configuration.
Embodiment 2
The present embodiment, as a example by Radix Cinnamomi porrecti Flos Carthami wood, carries out the assessment of regulation of absorbing dust capability, specifically comprises the following steps that
1, the electron-microscope scanning of Radix Cinnamomi porrecti Flos Carthami wood leaf surface fine structure
From Radix Cinnamomi porrecti Flos Carthami wood tree body choose appropriate blade, by process after in scanning electron microscope (S-570, day
This Hitachi) upper observation, observed result sees accompanying drawing 1.
Fig. 1 is the electron-microscope scanning figure of Radix Cinnamomi porrecti Flos Carthami wood blade lower surface；Wherein, A figure is Radix Cinnamomi porrecti, and B figure is Flos Carthami
Wood；Comparison diagram 1 is visible, and the lower epidermis concrete form of two kinds of plants shows themselves in that a small amount of cilium of following table leatherware of Radix Cinnamomi porrecti, and pore is circle
Shape, stomatal frequency is bigger；Flos Carthami wood following table leatherware cross cilium, pore be Long Circle and stomatal frequency bigger.
2, the mensuration of Photosynthetic parameter-Cond
Choosing upgrowth situation from Radix Cinnamomi porrecti Flos Carthami ebon body to be similar to, the consistent blade of leaf age and leaf position, through abundant photo-induction
After leading, utilize the portable instrument for determining photosynthesis Li-6400XT(U.S., Li-COR company) measure the two Cond value, measure
Result is respectively as follows: 0.0089 mol H2O m-2· s-1、0.1069 mol H2O m-2· s-1。
3, the assessment of the size of Radix Cinnamomi porrecti Flos Carthami wood regulation of absorbing dust capability
Finding according to electron-microscope scanning result and Cond pH-value determination pH, the blade lower epidermis of Radix Cinnamomi porrecti has a small amount of cilium, and Cond value is
0.0089 mol H2O m-2· s-1, less than 0.05 mol H2O m-2· s-1, and the following table leatherware cross of Flos Carthami wood blade
Shape cilium, pore is Long Circle, and stomatal frequency is relatively big, and Cond value is 0.1069 mol H simultaneously2O m-2· s-1, more than 0.1
mol H2O m-2· s-1, thus may determine that the regulation of absorbing dust capability of Flos Carthami wood is more than Radix Cinnamomi porrecti.
Claims (3)
1. the appraisal procedure of a plant regulation of absorbing dust capability, it is characterised in that comprise the steps:
(1) select fully deployed plant leaf blade, the lower epidermis of blade is used electron-microscope scanning, observes blade micro structure, including
The stomatal frequency of blade, and there is the situation of cilium；
(2) choose that the upgrowth situation observed with step (1) be similar, the consistent plant leaf blade of leaf age and leaf position, through fully
After photoinduction, plant leaf blade Photosynthetic parameter Cond stomatal conductance is measured；
(3) according to step (1) and the result of (2), plant regulation of absorbing dust capability is estimated；Blade lower epidermis has planting of cilium
Thing, its regulation of absorbing dust capability strengthens along with the increase of stomatal frequency value.
The appraisal procedure of a kind of plant regulation of absorbing dust capability the most according to claim 1, it is characterised in that: when Cond stomatal conductance
Value is less than 0.05 mol H2O m-2·s-1Time, plant regulation of absorbing dust capability is weak；Cond stomatal conductance value is at 0.05～0.10 mol H2O
m-2s-1Time, regulation of absorbing dust capability is medium；Cond stomatal conductance value is more than 0.1 mol H2O m-2·s-1Time, regulation of absorbing dust capability is strong.
3. the application of the appraisal procedure of plant regulation of absorbing dust capability described in claim 1, it is characterised in that for city, afforestation
Plant arrangement.
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| CN201610417089.0A CN106124697B (en) | 2016-06-12 | 2016-06-12 | A kind of appraisal procedure of plant regulation of absorbing dust capability and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770943A (en) * | 2016-12-12 | 2017-05-31 | 甘肃农业大学 | Potato stomatal conductance analyzer and its assay method |
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| CN103411846A (en) * | 2013-08-16 | 2013-11-27 | 塔里木大学 | Leaf surface dust fall quantity testing method based on hyperspectral technique |
| CN204241444U (en) * | 2014-10-17 | 2015-04-01 | 深圳市日昇园林绿化有限公司 | Lay the dust checkout equipment |
| CN105181516A (en) * | 2015-08-25 | 2015-12-23 | 青岛农业大学 | Determination method for shrub dust-retention amount |
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2016
- 2016-06-12 CN CN201610417089.0A patent/CN106124697B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103411846A (en) * | 2013-08-16 | 2013-11-27 | 塔里木大学 | Leaf surface dust fall quantity testing method based on hyperspectral technique |
| CN204241444U (en) * | 2014-10-17 | 2015-04-01 | 深圳市日昇园林绿化有限公司 | Lay the dust checkout equipment |
| CN105181516A (en) * | 2015-08-25 | 2015-12-23 | 青岛农业大学 | Determination method for shrub dust-retention amount |
Non-Patent Citations (1)
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| 刘璐等: "广州市常见行道树种叶片表面形态与滞尘能力", 《生态学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770943A (en) * | 2016-12-12 | 2017-05-31 | 甘肃农业大学 | Potato stomatal conductance analyzer and its assay method |
| CN106770943B (en) * | 2016-12-12 | 2023-02-03 | 甘肃农业大学 | Potato porosity conductivity tester and testing method thereof |
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| CN106124697B (en) | 2018-08-21 |
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