CN108181309A - A kind of assay method of plant carbon fixation and oxygen release amount - Google Patents
A kind of assay method of plant carbon fixation and oxygen release amount Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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- G01N2021/635—Photosynthetic material analysis, e.g. chrorophyll
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
The present invention relates to the assay method of the 13 kinds of common green plants carbon fixation and oxygen release amounts in Huhehaote City, which is：Step a, choice experiment material；In vitro plant is inserted into water by step b, sample treatment；Step c, the plant for selecting two plants of upgrowth situations similar, acquisition complete health blade is as laboratory sample；Step d, measures plant photosynthetic rate, and individual blade can be full of the plant of leaf chamber, be directly placed into leaf chamber and measure by step d1；Several individual blades cannot be full of the plant of leaf chamber, after close non-overlapping arrangement, be put into leaf chamber and measure by step d2；Step e calculates plant carbon fixation and oxygen release amount；Step e1, every sample measure the numerical value that 4 photosynthetic rates is taken to stablize every time；The Net Photosynthetic Rate average value measured is substituted into correlation formula and calculated by step e2；Step e3, interpretation of result.The present invention be the common green plants carbon fixation and oxygen release amount of semiarid region assay method, can comparative analysis go out the relatively high green plants of carbon fixation and oxygen release.
Description
Technical field
Detection field more particularly to a kind of plant carbon fixation and oxygen release amount the present invention relates to plant relevant physiological ecological index
Assay method.
Background technology
CO2Belong to one kind in greenhouse gases, due to CO2There can be the permeability of height to the shortwave from solar radiation,
And there is the absorbability of height to the long-wave radiation that the earth reflects, lead to global warming.Plant is with CO2As photosynthetic
The raw material of effect is natural absorption CO2Tool, reasonably can effectively alleviate greenhouse effects using urban greenery plants.CO2
It is one of raw material of photosynthesis of plant, O2It is one of photosynthetic product, but different regions not kindred plant, Various Seasonal
It will influence the power of photosynthesis of plant.Under this theory, for plant carbon fixation and oxygen release quantifier elimination, have been achieved for
Under achievement in research.
In the carbon fixation and oxygen release ability for studying plant, (1) studies area mostly based on the developed regions such as Beijing, Shanghai, Wuhan,
(2) research plant species is more single, and based on multidigit deciduous tree kind, mostly with cluster analysis, regression analysis is (3) analysis method
Main, (4) detection time is mostly a season or some months.
From different places, detection time section difference carbon fixation and oxygen release size is all different for plant life.For aiphyllium,
Still there is aerial part survival in winter, therefore aiphyllium still can carry out photosynthesis in winter, and tall for draft, fallen leaves
Wood, shrub, blade is gradually withered and yellow since cooling down autumn, and aboveground vegetation part starts to wither, the aerial part that can not survey.
Existing research is more the measurement to deciduous plant Growing season or summer, also incomplete to the detection of aiphyllium and draft
Face.
In view of drawbacks described above, creator of the present invention obtains this creation finally by prolonged research and practice.
Therefore, this research detects the common plant in 13 kinds of Huhehaote City of semiarid region from June, 2016 in May, 2017
Carbon fixation and oxygen release amount provides scientific basis for construction of city afforestation, alleviates due to excessive CO2Greenhouse effects of generation and relevant
Environmental problem.
Invention content
The purpose of the present invention is to provide a kind of assay method of plant carbon fixation and oxygen release amount, above-mentioned technology to be overcome to lack
It falls into.
To achieve the above object, the present invention provides a kind of assay method of plant carbon fixation and oxygen release amount, which is：
Step a, it is often experiment material with green plants to select Huhehaote City；Pass through the letter of the common green plants kind of access
Breath with reference to on-site inspection, filters out that totally 13 kinds of common green plants are examination including deciduous tree, aiphyllium, shrub, draft
Test material.
In vitro plant after picking is rapidly inserted into water by step b, sample treatment, to ensure that plant can be in a short time
Survival, and the time of measuring of this kind of plant was controlled within 5 minutes；
Step c, the acquisition of plant sample, the acquisition of magaphanerophytes select high sunny side blade, and ensure blade as far as possible
Measurement of correlation is carried out without incomplete, spot blade；The acquisition of shrub plant, acquisition be grown on aerial part top without it is incomplete,
The blade of spot carries out measurement of correlation；Herbal acquisition, the complete health blade for acquiring aerial part measure；Each
Two plants of plants for needing growth selection situation similar every time, each plant take 3 samples respectively；
Step d measures plant Net Photosynthetic Rate, using the portable photosynthetic instrument of LI-6400 in fine natural conditions
Under measure and the acquisition of related data；
Step d1, measure individual blade can be full of leaf chamber plant, as Xinjiang poplar, weeping willow, Chinese scholar tree, Fructus Forsythiae, lilac,
Intact leaf is directly put into leaf chamber and measures by Chinese herbaceous peony；
Step d2, the plant of leaf chamber cannot be full of by measuring individual blade, such as dragon spruce, Chinese pine, arbor-vitae, Chinese juniper, tawny daylily, precocity
Standing grain, savin juniper after the close non-overlapping arrangement of several blades, are put into leaf chamber and measure；
Step e calculates plant carbon fixation and oxygen release amount, utilizes the energy of the average value quantization plant carbon fixation and oxygen release of measured index
Power arranges the size of 13 kinds of plant carbon fixation and oxygen release amounts；
Step e1, every sample measure the numerical value that 4 photosynthetic rates is taken to stablize, the data dimension on photosynthetic instrument screen every time
It holds definite value or carries out value when having the variation less than 0.1；
The Net Photosynthetic Rate value measured is substituted into correlation computations formula and calculated, calculates net assimilation amount first, so by step e2
The formula for substituting into carbon fixation and oxygen release amount afterwards is calculated；
Step e3, interpretation of result use EXCEL2010 softwares, plot analysis, the size of arrangement plant carbon capacity.
Further, in above-mentioned steps a, mainly include：Deciduous tree：Xinjiang poplar, weeping willow, Chinese scholar tree；Aiphyllium：Cloud
China fir, Chinese pine, Chinese juniper, arbor-vitae；Shrub：Savin juniper, Fructus Forsythiae, lilac；Draft：Chinese herbaceous peony, tawny daylily, annual bluegrass.
Further, in above-mentioned steps c, the sample collection of each plant is required for growth selection in order similar two
Strain plant is as test specimen, and sample need to all acquire on this two plants of plant chosen every time.
Further, in above-mentioned steps e1, when acquiring Net Photosynthetic Rate numerical value, the net photosynthesis on photosynthetic instrument screen is waited until
It is being acquired after rate value stabilization.
Further, it in above-mentioned steps e2, is accordingly calculated with average value
According to measured Net Photosynthetic Rate, by following equation, carbon fixation and oxygen release amount is calculated：
Using the average value of the instantaneous photosynthetic rate of 13 kinds of measured plants of experiment, by the formula of following carbon fixation and oxygen release amount
Calculate different plant net assimilation amounts：
In formula：
P--- measures the assimilation total amount of day, mmolm-2·d-1；
Pi--- refer to the instantaneous photosynthetic rate of first measuring point, μm olm-2·s-1；
Pi+1--- for the instantaneous photosynthetic rate of next measuring point, μm olm-2·s-1；
ti--- for first measuring point time, h；
Ti+1 be next measuring point time, h；
3600 refer to per hour for 3600s, and 1000 finger 1mmol are 1000 μm of ol.
Using the net assimilation amount calculated carbon capacity is calculated by following equation：
Wco2=P (1-0.2) × 44/1000
In formula：
44---CO2Molal weight, gmol-1；
Wco2--- the blade of unit area fixes CO2Quality, gm-2·d-1；
Using the net assimilation amount and carbon capacity calculated oxygen release amount is calculated by following equation：
Wo2=P (1-0.2) × 32/1000
In formula：
WO2--- day discharges O2Amount, [g/ (m2·d)]；
32---O2Molal weight, gmol-1
Compared with prior art the beneficial effects of the present invention are：The present invention has detected semiarid zone and commonly uses greening plant
The carbon fixation and oxygen release efficiency of object a year and a day.A kind of measure side of carbon fixation and oxygen release amount can be provided to semiarid region by common green plants
Method.Curve is drawn with EXCEL and is analyzed.
Description of the drawings
Fig. 1 is the flow chart of plant carbon fixation and oxygen release quantity measuring method of the present invention；
Fig. 2 is that the carbon capacity of different vegetation types changes；
Fig. 3 is that the carbon capacity of different plants compares；
Specific embodiment
Below in conjunction with attached drawing, the forgoing and additional technical features and advantages are described in more detail.
The detailed process of the assay method of plant carbon fixation and oxygen release amount of the present invention is；
Step a, it is often experiment material with green plants to select Huhehaote City；
The information of common green plants kind is consulted, with reference to on-site inspection, is planted in Hohhot district selection containing all measure
The region of species, it is experiment material that totally 13 kinds of common green plants are filtered out from aiphyllium, deciduous tree, shrub, draft
Material, respectively dragon spruce, Chinese pine, Chinese juniper, arbor-vitae；Xinjiang poplar, weeping willow, Chinese scholar tree；Savin juniper, Fructus Forsythiae, lilac；Chinese herbaceous peony, tawny daylily,
Annual bluegrass.
Step b, sample treatment；
When acquiring required plant sample, pay attention to that complete blade construction, the best band of sample blade of acquisition cannot be destroyed
Branch.In vitro plant after picking is inserted into water, ensures that plant can survive in a short time, the plant was completed within 5 minutes
Measurement；
Step c, the acquisition of plant sample；The acquisition of magaphanerophytes selects the blade at sunny side tree crown, and ensures as far as possible
Blade carries out measurement of correlation without incomplete, spot blade；The acquisition of shrub plant, acquisition are grown on aerial part sunny side top
Measurement of correlation is carried out without incomplete, spot blade；Herbal acquisition, the complete health blade for acquiring aerial part are surveyed
Amount；Each needs the two plant plants similar to upgrowth situation every time, and each plant takes 3 samples respectively, to select the nothing of health
Two plants of plant of apparent pest and disease damage；
Step d measures plant Net Photosynthetic Rate,
Step d1, measure individual blade can be full of leaf chamber plant, as Xinjiang poplar, weeping willow, Chinese scholar tree, Fructus Forsythiae, lilac,
Intact leaf is directly put into leaf chamber and measures by Chinese herbaceous peony；
Step d2, the plant of leaf chamber cannot be full of by measuring individual blade, such as dragon spruce, Chinese pine, arbor-vitae, Chinese juniper, tawny daylily, precocity
Standing grain, savin juniper after the close non-overlapping arrangement of several blades, are put into leaf chamber and measure；
Step e calculates plant carbon fixation and oxygen release amount, utilizes the energy of the average value quantization plant carbon fixation and oxygen release of measured index
Power arranges the size of 13 kinds of plant carbon fixation and oxygen release amounts；
Step e1, every sample measure the numerical value that 4 photosynthetic rates is taken to stablize, the data dimension on photosynthetic instrument screen every time
It holds definite value or carries out value when having the variation less than 0.1；
The instantaneous photosynthetic rate value measured is substituted into correlation computations formula and calculated, calculates net assimilation amount first by step e2,
Then the formula for substituting into carbon fixation and oxygen release amount is calculated；
The average value of the instantaneous photosynthetic rate of 13 kinds of measured plants will be tested, based on the formula of following carbon fixation and oxygen release amount
Calculate different plant net assimilation amounts：
In formula：
P--- measures the assimilation total amount of day, mmolm-2·d-1；
Pi--- refer to the instantaneous photosynthetic rate of first measuring point, μm olm-2·s-1；
Pi+1--- for the instantaneous photosynthetic rate of next measuring point, μm olm-2·s-1；
ti--- for first measuring point time, h；
Ti+1 be next measuring point time, h；
3600 refer to per hour for 3600s, and 1000 finger 1mmol are 1000 μm of ol.
Using the net assimilation amount calculated carbon capacity is calculated by following equation：
Wco2=P (1-0.2) × 44/1000
In formula：
44---CO2Molal weight, gmol-1；
Wco2--- the blade of unit area fixes CO2Quality, gm-2·d-1；
Using the net assimilation amount and carbon capacity calculated oxygen release amount is calculated by following equation：
Wo2=P (1-0.2) × 32/1000
In formula：
WO2--- day discharges O2Amount, [g/ (m2·d)]；
32---O2Molal weight, gmol-1
Step e3, interpretation of result, according to the formula of above-mentioned carbon fixation and oxygen release amount be calculated as a result, using EXCEL2010
Software, plot analysis, the size of arrangement plant carbon capacity.
By above-mentioned formula it can be seen that the carbon capacity of plant and oxygen release amount are come by the same derivation of equation, to avoid
It repeats, only lists the interpretation of result to plant carbon capacity.
(1) interpretation of result obtains the size of 13 kinds of plant carbon capacities
The plant carbon capacity present invention obtains the plant after substituting into correlation formula with the instantaneous photosynthetic rate of measured plant
Then carbon capacity is compared again；
The plant that the present invention acquires is divided into four classes, respectively aiphyllium：Dragon spruce, Chinese pine, Chinese juniper, arbor-vitae；Deciduous tree：Newly
Boundary poplar, weeping willow, Chinese scholar tree；Shrub：Savin juniper, Fructus Forsythiae, lilac；Draft：Chinese herbaceous peony, annual bluegrass.It calculates analysis and obtains evergreen Qiao
Wood：124.9216947g·m-2·d-1, deciduous tree：56.595g·m-2·d-1Draft：50.208g·m-2·d-1Shrub：
74.060g·m-2·d-1。
It please refers to shown in Fig. 2, is the carbon capacity variation of different vegetation types after survey calculation of the present invention；The result shows that
Four kinds of different types of plants reached peak value on 2nd in September in 2016.Since aiphyllium still has aerial part to deposit in winter
It is living, therefore the carbon capacity highest of aiphyllium；Also there is aerial part survival, therefore shrub is consolidated during savin juniper winter in shrub
Carbon amounts is secondly.
(2) the year variation of different plant carbon fixation and oxygen release abilities；
It please refers to shown in Fig. 3, is the carbon capacity variation of 13 kinds of plants, as a result show the carbon capacity of arbor-vitae in aiphyllium
It is highest in 13 kinds of plants, and the arbor-vitae, Chinese juniper, Chinese pine carbon capacity that arrange front three are not much different.Tawny daylily in herbaceous plant
Carbon capacity be minimum in 13 kinds of plants, and the carbon capacity of annual bluegrass and tawny daylily is not much different.
Plant is with CO2Photosynthesis, different floristics, the difference in ecological functions are carried out for raw material；Plant growth
Region is different, and season is different, these internal and external factors can all influence the carbon fixation and oxygen release ability of plant.By comparing different plant absorptions
CO2Discharge O2Ability, the green plants of high carbon fixation and oxygen release amount can be selected, establish different types of urban afforestation system, can
Effectively slow down the generation of greenhouse effects.
The foregoing is merely presently preferred embodiments of the present invention, is merely illustrative for invention, and not restrictive.
Those skilled in the art understands, many changes can be carried out to it in the spirit and scope limited in invention claim, changes,
It is even equivalent, but fall in protection scope of the present invention.
Claims (7)
1. the assay method of the common green plants carbon fixation and oxygen release amount in 13 kinds of Huhehaote City, which is characterized in that the detailed process is：
Step a, it is often experiment material with green plants to select Huhehaote City；The information of green plants kind is commonly used by access,
With reference to on-site inspection, it is experiment that totally 13 kinds of common green plants are filtered out from aiphyllium, deciduous tree, shrub, draft
Material；
In vitro plant after picking is inserted into water by step b, sample treatment, ensures that plant can survive in a short time, at 5 points
The measurement of the plant is completed within clock；
Step c, the acquisition of plant sample, the acquisition of magaphanerophytes select high sunny side blade, and ensure blade without residual as far as possible
Lack, the blade of spot carries out measurement of correlation, the acquisition of shrub plant, acquisition be grown on aerial part top without incomplete, spot
Blade carry out measurement of correlation；Herbal acquisition, the complete health blade for acquiring aerial part measure；Each is every time
The two plant plants similar to upgrowth situation are needed, each plant takes 3 samples respectively；
Step d is measured plant photosynthetic rate, is carried out under fine natural conditions using the portable photosynthetic instrument of LI-6400
Experiment；
Step d1, the plant of leaf chamber can be full of by measuring individual blade, such as Xinjiang poplar, weeping willow, Chinese scholar tree, Fructus Forsythiae, lilac, Chinese herbaceous peony
Intact leaf is directly put into leaf chamber and measures by medicine；
Step d2, measure individual blade cannot be full of leaf chamber plant, as dragon spruce, Chinese pine, arbor-vitae, Chinese juniper, tawny daylily, annual bluegrass,
Savin juniper after the close non-overlapping arrangement of several blades, is put into leaf chamber and measures；
Step e calculates plant carbon fixation and oxygen release amount；
Step e1, every sample measure the numerical value that 4 photosynthetic rates is taken to stablize every time, when the data on photosynthetic instrument screen remain fixed
It is worth or carries out value when having the variation less than 0.1；
The instantaneous photosynthetic rate value average value measured is substituted into correlation formula and calculated, calculates net assimilation amount first, so by step e2
The formula that net assimilation amount is substituted into carbon fixation and oxygen release amount afterwards calculates；
Step e3, interpretation of result, using SPSS19.0 and EXCEL2010 softwares, plot analysis arranges the big of plant carbon capacity
It is small.
2. the assay method of plant carbon fixation and oxygen release amount according to claim 1, which is characterized in that in above-mentioned steps a, adjust
After looking into research Huhehaote City's green plants kind, common Xinjiang poplar, weeping willow, Chinese scholar tree have been selected；Dragon spruce, Chinese pine, arbor-vitae, circle
Cypress；Savin juniper, Fructus Forsythiae, lilac；Chinese herbaceous peony, tawny daylily, annual bluegrass are experiment material.
3. the assay method of plant carbon fixation and oxygen release amount according to claim 1, which is characterized in that in above-mentioned steps b, sample
Product processing.Sample is not disposably to acquire, but completes to adopt after to a kind of measurement of sample in vitro 5 minutes of sample successively
Collection.
4. the assay method of plant carbon fixation and oxygen release amount according to claim 1, which is characterized in that in above-mentioned steps c, plant
The acquisition of object sample, arbor, shrub, draft are required for acquisition top sunny side health, immaculate healthy leaves.
5. the assay method of plant carbon fixation and oxygen release amount according to claim 1, which is characterized in that in above-mentioned steps d2,
To that cannot be full of the plant of leaf chamber, under small as possible blade overlapping being put into leaf chamber measures.
6. the assay method of plant carbon fixation and oxygen release amount according to claim 1, which is characterized in that in above-mentioned steps e2, profit
With the average value of the instantaneous photosynthetic rate of 13 kinds of measured plants of experiment, different plants are calculated by the formula of following carbon fixation and oxygen release amount
Object net assimilation amount：
In formula：
P--- measures the assimilation total amount of day, mmolm-2·d-1；
Pi--- refer to the instantaneous photosynthetic rate of first measuring point, μm olm-2·s-1；
Pi+1--- for the instantaneous photosynthetic rate of next measuring point, μm olm-2·s-1；
ti--- for first measuring point time, h；
Ti+1 be next measuring point time, h；
3600 refer to per hour for 3600s, and 1000 finger 1mmol are 1000 μm of ol；
Using the net assimilation amount calculated carbon capacity is calculated by following equation：
In formula：
44---CO2Molal weight, gmol-1；
--- the blade of unit area fixes CO2Quality, gm-2·d-1；
Using the net assimilation amount and carbon capacity calculated oxygen release amount is calculated by following equation：
In formula：
WO2--- day discharges O2Amount, [g/ (m2·d)]；
32---O2Molal weight, gmol-1。
7. the assay method of plant carbon fixation and oxygen release amount according to claim 6, which is characterized in that in above-mentioned steps e3,
When to 13 kinds of plant carbon fixation and oxygen release abilities and correlation analysis, with the instantaneous photosynthetic rate of measured plant substitute into correlation formula into
The calculating of row carbon fixation and oxygen release amount draws and the carbon capacity of 13 kinds of plants is ranked up.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110710419A (en) * | 2019-07-27 | 2020-01-21 | 中建科技有限公司 | Optimization method for building three-dimensional greening |
| CN110715682A (en) * | 2019-07-27 | 2020-01-21 | 中建科技有限公司 | Greening building monitoring method and system |
| CN114216879A (en) * | 2021-12-14 | 2022-03-22 | 交通运输部公路科学研究所 | Portable device for measuring photosynthetic gas in plant photosynthesis |
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| CN104820065A (en) * | 2015-04-15 | 2015-08-05 | 上海植物园 | Carbon sequestration measuring and calculating method for urban single plant tree |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110710419A (en) * | 2019-07-27 | 2020-01-21 | 中建科技有限公司 | Optimization method for building three-dimensional greening |
| CN110715682A (en) * | 2019-07-27 | 2020-01-21 | 中建科技有限公司 | Greening building monitoring method and system |
| CN114216879A (en) * | 2021-12-14 | 2022-03-22 | 交通运输部公路科学研究所 | Portable device for measuring photosynthetic gas in plant photosynthesis |
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