CN106124590A - A kind of preparation method of electrochemistry Malathion sensor - Google Patents
A kind of preparation method of electrochemistry Malathion sensor Download PDFInfo
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Abstract
The invention discloses the preparation method of a kind of electrochemical sensor built based on nano-functional material.The method comprises the steps of firstly, preparing a kind of New Two Dimensional nano-electrode material load type double-metal co-doped nano composite FeMn TiO2/MoS2I.e. ferrum, the two-dimensional nano composite of manganese codoped nano titania square In-situ reaction molybdenum bisuphide, utilize the good biocompatibility of this material and big specific surface area, Malathion antibody in load, when detecting, due to ferrum, manganese codoped titanium dioxide can be with catalyzing hydrogen peroxide in-situ preparation O2, producing electrochemical signals, recycling antibody is combined the impact on electron transport ability with the specific quantification of antigen so that current intensity reduces accordingly, finally achieves the structure of the biosensor using unmarked electrochemical method detection Malathion.
Description
Technical field
The present invention relates to the preparation method of a kind of electrochemical sensor built based on nano-functional material.Belong to Performances of Novel Nano-Porous
Rice functional material and biosensor technology field.
Background technology
Malathion (malathion), another name horse traction match is high, is a kind of effective drug agent, it is adaptable to preventing and treating Nicotiana tabacum L., tea and
Insect on the crops such as mulberry.Malathion has good tagging, stomach toxicity and certain fumigation action, without systemic action.?
In soil, Malathion slightly can be migrated to deep subsoil by the eluviation of water.Malathion in soil can pass through
Plant root absorbs and enters in plant.People eat by mistake this kind of plant or containing its residue plant after, Malathion energy
Enter human body by digestive tract, respiratory tract and complete skin and mucosa, it may appear that Nausea and vomiting, have a headache, have loose bowels, whole body soft
The weak unable preliminary symptom of poisoning such as grade, Long Term Contact can cause canceration.
At present, the method for detection Malathion mainly has chromatography, mass spectrography etc..This type of method instrument is valuable, operation is multiple
Miscellaneous, laboratory personnel just can detect after needing professional training.Therefore, R&D costs are low, detect fast, highly sensitive, specificity
Strong Malathion sensor is significant.
Electrochemica biological sensor is widely used in facing due to advantages such as it are highly sensitive, specificity is good, easy and simple to handle
The fields such as bed diagnosis, pharmaceutical analysis, environmental monitoring.The most especially more with the research of unmarked electrochemical immunosensor, it is crucial
Technology be to improve the fixed amount of modified electrode antagonist and to the test signal response speed of end liquid and size.Titanium dioxide is
A kind of photocatalyst material being most widely used, simultaneously because good biocompatibility, is also commonly used as electrode matrix material.By
Can expose more high miller index surface in sheet-like titanium dioxide nanomaterial, have higher catalysis activity, titanium dioxide is received
Rice sheet has ratio nanoparticle preferably application prospect, and the research for titanium dioxide nanoplate also receives much concern.But, dioxy
The sensitivity that change titanium poorly conductive also limit the electrochemical sensor built by single titanium dioxide nano material is the highest,
It is unfavorable for actual application.Semiconductor nano material is modified or is combined special nano material, on the one hand adds electrode ratio
Surface area, strengthens electrodes conduct ability, and on the other hand the two can produce concerted catalysis effect, and bigger enhancing is to hydrogen peroxide
Solution H2O2Catalytic response speed and current responsing signal size, be greatly improved detection sensitivity.Therefore, design, prepare height
Effect, stable titanium dioxide nanoplate and trim thereof are the key technologies preparing electrochemical sensor.
(chemical formula is MoS to molybdenum bisuphide2) nano material, there is two-dimensional layered structure, be most widely used solid profit
One of lubrication prescription.Lamellar two-dimension nano materials after its stripping, is the semiconductor nano material of excellent performance, except having big ratio
Surface area, can improve load capacity as catalyst and the carrier of biological antibody, also has simultaneously as promoter excellent
Electron transmission performance.
At present, most synthesizing mean be all be separately synthesized after, then catalyst is combined with carrier, process is numerous
Trivial, productivity is the highest.Therefore, for In-situ reaction preparation, there is the two dimensional nano electrodes material of good electric chemical property to have extensively
Application prospect and important scientific meaning.
Summary of the invention
It is an object of the invention to provide a kind of prepare simple, highly sensitive, detection quickly, the electrochemistry horse of high specificity
Draw the preparation method of sulfur phosphorus sensor, prepared sensor, can be used for quick, the Sensitive Detection of Malathion.Based on this mesh
, the method comprises the steps of firstly, preparing a kind of New Two Dimensional nano-electrode material load type double-metal co-doped nano composite
FeMn-TiO2/MoS2, i.e. ferrum, the two-dimensional nano composite wood of manganese codoped nano titania square In-situ reaction molybdenum bisuphide
Material, utilizes the good biocompatibility of this material and big specific surface area, Malathion antibody in load, is detecting
Time, due to ferrum, manganese codoped titanium dioxide can be with catalyzing hydrogen peroxide in-situ preparation O2, producing electrochemical signals, recycling is anti-
Body is combined the impact on electron transport ability with the specific quantification of antigen so that current intensity reduces accordingly, finally achieves
Use the structure of the biosensor of unmarked electrochemical method detection Malathion.
The technical solution used in the present invention is as follows:
1. the preparation method of an electrochemistry Malathion sensor, it is characterised in that described electrochemistry Malathion sensing
Device is by working electrode, FeMn-TiO2/MoS2, Malathion antibody, bovine serum albumin composition;Described FeMn-TiO2/MoS2
Two-dimensional nano composite for ferrum, manganese codoped nano titania square and molybdenum bisuphide In-situ reaction;
It is characterized in that, described preparation method includes following preparation process:
A. FeMn-TiO is prepared2/MoS2;
B. electrochemistry Malathion sensor is prepared;
Wherein, step a prepares FeMn-TiO2/MoS2Concretely comprise the following steps:
(1) take 0.6 g molybdenum disulfide powder, 0.2 ~ 2.0 mmol iron salt and 0.2 ~ 2.0 mmol manganese salt jointly join 3 ~
In 10 mL n-butyllithium solutions, at nitrogen protection and 30 ~ 60 DEG C, stir 12 ~ 48 hours, obtain reacted molten
Liquid;
(2) utilize reacted solution in non-polar solven washing step (1), at 30 ~ 60 DEG C, then carry out water bath sonicator
Process, after having processed, the solution after recycling non-polar solven carrying out washing treatment, vacuum drying, obtain two sulfur of ferrum, manganese intercalation altogether
Change molybdenum nano material;
(3) the molybdenum disulfide nano material taking the prepared ferrum of 10 ~ 500 mg steps (2), manganese intercalation altogether joins 5 mL metatitanic acids
In four butyl esters, after stirring 1 hour, it is slowly added to 0.5 ~ 0.8 mL Fluohydric acid. while stirring, then instead at 160 ~ 200 DEG C
Answer in still and react 18 ~ 24 hours;
(4) by the product of step (3) gained, after ultra-pure water and dehydrated alcohol centrifuge washing three times, at 50 DEG C, vacuum is done
Dry, i.e. prepare FeMn-TiO2/MoS2;
Described n-butyllithium solution is the hexane solution of n-BuLi, and concentration is 1.6 mol/L;
Described iron salt is selected from one of following: iron sulfate, iron chloride, ferric nitrate, ferric acetate, organoiron compound;
Described manganese salt is selected from one of following: manganese sulfate, manganese chloride, manganese nitrate, manganese acetate, organo-manganese compound;
Described non-polar solven is selected from one of following: hexane, hexamethylene, carbon tetrachloride, benzene, toluene;
Described water bath sonicator processes, and the process time is 1 hour;
Step b prepares concretely comprising the following steps of electrochemistry Malathion sensor:
(1) with ITO electro-conductive glass as working electrode, at the FeMn-TiO of electrode surface drop coating 8 ~ 12 L2/MoS2Colloidal sol, room temperature
Under dry;
(2) the electrode buffer solution PBS that will obtain in step (1), continues at electrode surface drop coating 8 ~ 12 L 10
The Malathion antibody-solutions of g/mL, preserves in 4 DEG C of refrigerators and dries;
(3) the electrode PBS that will obtain in step (2), continuing in electrode surface drop coating 8 ~ 12 L concentration is 100 g/
The bovine serum albumin solution of mL, preserves in 4 DEG C of refrigerators and dries;
(4) the electrode PBS that will obtain in step (3), preserves after drying in 4 DEG C of refrigerators, i.e. prepares electrochemistry and passes
Sensor;
Described FeMn-TiO2/MoS2Colloidal sol is by the FeMn-TiO of 50 mg2/MoS2Powder is dissolved in 10 mL ultra-pure waters, and
The hydrosol prepared after ultrasonic 30 min;
Described PBS is the phosphate buffered solution of 10 mmol/L, and the pH value of described phosphate buffered solution is 7.4.
The application of the electrochemistry Malathion sensor prepared by preparation method the most of the present invention, it is characterised in that
Including following applying step:
A. standard solution preparation: prepare the Malathion standard solution of one group of variable concentrations including blank standard specimen;
B. working electrode is modified: by the electrochemistry Malathion sensor prepared by preparation method as claimed in claim 1 be
Working electrode, by the Malathion standard solution of the variable concentrations of preparation drop coating respectively in step a to working electrode surface, 4 DEG C
Refrigerator preserves;
C. working curve is drawn: using saturated calomel electrode electrode as reference electrode, platinum electrode is as to electrode, with step b
The working electrode composition three-electrode system modified, connects electrochemical workstation, successively adds 15 mL PBS in a cell
H with 20 L 5 mol/L2O2;By the working electrode of chronoamperometry detection assembling to H2O2Response;Blank standard specimen
Response current is designated asI 0, the response current of the Malathion standard solution containing variable concentrations is denoted asI i, response current reduces
Difference is ΔI = I 0-I i, ΔIMass concentration with Malathion standard solutionCBetween linear, draw ΔI
-CWorking curve;
D. the detection of Malathion: replace the Malathion standard solution in step a with testing sample, according in step b and c
Method detect, according to response electrical signal intensity reduce difference DELTAIAnd working curve, obtain horse traction in testing sample
The content of sulfur phosphorus.
The useful achievement of the present invention
(1) sensor preparation in electrochemistry Malathion of the present invention is simple, easy to operate, it is achieved that to sample quick,
Selective enumeration method sensitive, high, and low cost, can be applicable to portable inspectiont, has market development prospect;
(2) present invention uses the method for In-situ reaction to be prepared for New Two Dimensional nano-electrode material FeMn-TiO first2/MoS2,
The method mainly has three advantages: one is, due to ferrum, the manganese growth in situ on nano titania square jointly fully with
Nano titania square contacts, and utilizes the metal surface plasma body effect of ferrum, manganese, is effectively increased semiconductor substrate electronics
Transmission capacity and catalysis activity, although solving, titanium dioxide nanoplate specific surface area is bigger and mesoporous characteristic is applicable to electrification
Host material, but the technical problem that electro-chemical activity is the highest and current signal is unstable;Two are, due to molybdenum bisuphide lamellar
The load characteristic of two-dimension nano materials and nano titania square thereon fully dispersed, greatly increases electron transmission
Ability, solves titanium dioxide nanoplate poorly conductive and current responsing signal is weak and be unfavorable for preparing the skill of electrochemical sensor
Art problem;Three are, due to Fe and Mn the most not only as intercalation material but also as reaction dopant material, finally use
The method of In-situ reaction achieves one pot of preparation of this composite, not only saves time, spillage of material, and makes preparation
Ferrum, manganese codoped nano titania square can preferably evenly spread on molybdenum bisuphide lamellar two-dimension nano materials
Face.Therefore, effective preparation of this material, there is important scientific meaning and using value;
(3) present invention is first by FeMn-TiO2/MoS2It is applied in the preparation of electrochemistry Malathion sensor, significantly improves
The strength and stability of current signal, substantially increases the detection sensitivity of electrochemical sensor so that electrochemica biological passes
Sensor achieves the application in real work;The application of this material, is also associated biomolecule sensor, as Optical Electro-Chemistry senses
Device, Electrochemiluminescsensor sensor etc. provide Technical Reference, have the most potential use value.
Detailed description of the invention
Embodiment 1 FeMn-TiO2/MoS2Preparation
(1) taking 0.6 g molybdenum disulfide powder, 0.2 mmol iron salt and 0.2 mmol manganese salt, jointly to join 3mL n-BuLi molten
In liquid, at nitrogen protection and 60 DEG C, stir 12 hours, obtain reacted solution;
(2) utilize reacted solution in non-polar solven washing step (1), at 60 DEG C, then carry out water bath sonicator process,
After having processed, the solution after recycling non-polar solven carrying out washing treatment, vacuum drying, obtain the molybdenum bisuphide of ferrum, manganese intercalation altogether
Nano material;
(3) the molybdenum disulfide nano material taking the prepared ferrum of 500 mg steps (2), manganese intercalation altogether joins 5 mL butyl titanates
In, after stirring 1 hour, it is slowly added to 0.5 mL Fluohydric acid. while stirring, then reacts 18 hours in a kettle. at 160 DEG C;
(4) by the product of step (3) gained, after ultra-pure water and dehydrated alcohol centrifuge washing three times, at 50 DEG C, vacuum is done
Dry, i.e. prepare FeMn-TiO2/MoS2;
Described n-butyllithium solution is the hexane solution of n-BuLi, and concentration is 1.6 mol/L;
Described iron salt is iron sulfate;
Described manganese salt is manganese sulfate;
Described non-polar solven is hexane;
Described water bath sonicator processes, and the process time is 1 hour.
Embodiment 2 FeMn-TiO2/MoS2Preparation
(1) taking 0.6 g molybdenum disulfide powder, 1.0 mmol iron salt and 1.0 mmol manganese salt, jointly to join 5 mL n-BuLis molten
In liquid, at nitrogen protection and 30 DEG C, stir 24 hours, obtain reacted solution;
(2) utilize reacted solution in non-polar solven washing step (1), at 30 DEG C, then carry out water bath sonicator process,
After having processed, the solution after recycling non-polar solven carrying out washing treatment, vacuum drying, obtain the molybdenum bisuphide of ferrum, manganese intercalation altogether
Nano material;
(3) the molybdenum disulfide nano material taking the prepared ferrum of 200 mg steps (2), manganese intercalation altogether joins 5 mL butyl titanates
In, after stirring 1 hour, it is slowly added to 0.6 mL Fluohydric acid. while stirring, then reacts 20 hours in a kettle. at 180 DEG C;
(4) by the product of step (3) gained, after ultra-pure water and dehydrated alcohol centrifuge washing three times, at 50 DEG C, vacuum is done
Dry, i.e. prepare FeMn-TiO2/MoS2;
Described n-butyllithium solution is the hexane solution of n-BuLi, and concentration is 1.6 mol/L;
Described iron salt is iron chloride;
Described manganese salt is manganese chloride;
Described non-polar solven is carbon tetrachloride;
Described water bath sonicator processes, and the process time is 1 hour.
Embodiment 3 FeMn-TiO2/MoS2Preparation
(1) take 0.6 g molybdenum disulfide powder, 2.0 mmol iron salt and 2.0 mmol manganese salt and jointly join 10 mL n-BuLis
In solution, at nitrogen protection and 50 DEG C, stir 48 hours, obtain reacted solution;
(2) utilize reacted solution in non-polar solven washing step (1), at 50 DEG C, then carry out water bath sonicator process,
After having processed, the solution after recycling non-polar solven carrying out washing treatment, vacuum drying, obtain the molybdenum bisuphide of ferrum, manganese intercalation altogether
Nano material;
(3) the molybdenum disulfide nano material taking the prepared ferrum of 10 mg steps (2), manganese intercalation altogether joins 5 mL butyl titanates
In, after stirring 1 hour, it is slowly added to 0.8 mL Fluohydric acid. while stirring, then reacts 24 hours in a kettle. at 200 DEG C;
(4) by the product of step (3) gained, after ultra-pure water and dehydrated alcohol centrifuge washing three times, at 50 DEG C, vacuum is done
Dry, i.e. prepare FeMn-TiO2/MoS2;
Described n-butyllithium solution is the hexane solution of n-BuLi, and concentration is 1.6 mol/L;
Described iron salt is ferric acetate;
Described manganese salt is manganese acetate;
Described non-polar solven is benzene;
Described water bath sonicator processes, and the process time is 1 hour.
The preparation method of embodiment 4 electrochemistry Malathion sensor
(1) with glass-carbon electrode as working electrode, at the FeMn-TiO of electrode surface drop coating 8 L2/MoS2Colloidal sol, dries in the air under room temperature
Dry;
(2) the electrode buffer solution PBS that will obtain in step (1), continues at electrode surface drop coating 8 L 10 g/mL
Malathion antibody-solutions, 4 DEG C of refrigerators preserve and dry;
(3) the electrode PBS that will obtain in step (2), continuing in electrode surface drop coating 8 L concentration is 100 g/mL
Bovine serum albumin solution, 4 DEG C of refrigerators preserve and dry;
(4) the electrode PBS that will obtain in step (3), preserves in 4 DEG C of refrigerators after drying, i.e. prepares electrochemistry horse
Draw sulfur phosphorus sensor;
Described FeMn-TiO2/MoS2Colloidal sol is by the FeMn-TiO of 50 mg2/MoS2Powder is dissolved in 10 mL ultra-pure waters, and
The hydrosol prepared after ultrasonic 30 min;
Described PBS is the phosphate buffered solution of 10mmol/L, and the pH value of described phosphate buffered solution is 7.4.
The preparation method of embodiment 5 electrochemistry Malathion sensor
(1) with glass-carbon electrode as working electrode, at the FeMn-TiO of electrode surface drop coating 10 L2/MoS2Colloidal sol, dries in the air under room temperature
Dry;
(2) the electrode buffer solution PBS that will obtain in step (1), continues at electrode surface drop coating 10 L 10 g/
The Malathion antibody-solutions of mL, preserves in 4 DEG C of refrigerators and dries;
(3) the electrode PBS that will obtain in step (2), continuing in electrode surface drop coating 10 L concentration is 100 g/mL
Bovine serum albumin solution, 4 DEG C of refrigerators preserve and dry;
(4) the electrode PBS that will obtain in step (3), preserves in 4 DEG C of refrigerators after drying, i.e. prepares electrochemistry horse
Draw sulfur phosphorus sensor;
Described FeMn-TiO2/MoS2Colloidal sol is by the FeMn-TiO of 50 mg2/MoS2Powder is dissolved in 10 mL ultra-pure waters, and
The hydrosol prepared after ultrasonic 30 min;
Described PBS is the phosphate buffered solution of 10 mmol/L, and the pH value of described phosphate buffered solution is 7.4.
The preparation method of embodiment 6 electrochemistry Malathion sensor
(1) with glass-carbon electrode as working electrode, at the FeMn-TiO of electrode surface drop coating 12 L2/MoS2Colloidal sol, dries in the air under room temperature
Dry;
(2) the electrode buffer solution PBS that will obtain in step (1), continues at electrode surface drop coating 12 L 10 g/
The Malathion antibody-solutions of mL, preserves in 4 DEG C of refrigerators and dries;
(3) the electrode PBS that will obtain in step (2), continuing in electrode surface drop coating 12 L concentration is 100 g/mL
Bovine serum albumin solution, 4 DEG C of refrigerators preserve and dry;
(4) the electrode PBS that will obtain in step (3), preserves in 4 DEG C of refrigerators after drying, i.e. prepares electrochemistry horse
Draw sulfur phosphorus sensor;
Described FeMn-TiO2/MoS2Colloidal sol is by the FeMn-TiO of 50 mg2/MoS2Powder is dissolved in 10 mL ultra-pure waters, and
The hydrosol prepared after ultrasonic 30 min;
Described PBS is the phosphate buffered solution of 10 mmol/L, and the pH value of described phosphate buffered solution is 7.4.
The electrochemistry Malathion sensor of embodiment 7 embodiment 1 ~ 6 preparation, is applied to the detection of Malathion, step
Rapid as follows:
(1) standard solution preparation: prepare the Malathion standard solution of one group of variable concentrations including blank standard specimen;
(2) working electrode is modified: by the electrochemistry Malathion sensor prepared by preparation method as claimed in claim 1 be
Working electrode, by the Malathion standard solution of the variable concentrations of preparation drop coating respectively in step (1) to working electrode surface, 4
DEG C refrigerator preserves;
(3) working curve is drawn: using saturated calomel electrode electrode as reference electrode, platinum electrode is as to electrode, with step b
The working electrode composition three-electrode system modified, connects electrochemical workstation, successively adds 15 mL PBS in a cell
H with 20 L 5 mol/L2O2;By the working electrode of chronoamperometry detection assembling to H2O2Response;Blank standard specimen
Response current is designated asI 0, the response current of the Malathion standard solution containing variable concentrations is denoted asI i, response current reduces
Difference is ΔI = I 0-I i, ΔIMass concentration with Malathion standard solutionCBetween linear, draw ΔI
-CWorking curve;The linear detection range of Malathion is: 0.009 ~ 200 ng/mL, and detection is limited to: 3.0 pg/mL;
(4) detection of Malathion: replace the Malathion standard solution in step (1) with testing sample, according to step (2) and
(3) method in detects, the difference DELTA reduced according to response electrical signal intensityIAnd working curve, obtain in testing sample
The content of Malathion.
Claims (4)
1. the preparation method of an electrochemistry Malathion sensor, it is characterised in that described electrochemistry Malathion sensor
By working electrode, FeMn-TiO2/MoS2, Malathion antibody, bovine serum albumin composition;Described FeMn-TiO2/MoS2For
Ferrum, manganese codoped nano titania square and the two-dimensional nano composite of molybdenum bisuphide In-situ reaction.
2. the preparation method of electrochemistry Malathion as claimed in claim 1 sensor, it is characterised in that described preparation side
Method includes following two preparation process:
A. FeMn-TiO is prepared2/MoS2;
B. electrochemistry Malathion sensor is prepared.
3. the preparation method of the electrochemistry Malathion sensor as described in claim 1 and 2, it is characterised in that described
FeMn-TiO2/MoS2Concrete preparation process be:
(1) take 0.6 g molybdenum disulfide powder, 0.2 ~ 2.0 mmol iron salt and 0.2 ~ 2.0 mmol manganese salt jointly join 3 ~
In 10 mL n-butyllithium solutions, at nitrogen protection and 30 ~ 60 DEG C, stir 12 ~ 48 hours, obtain reacted molten
Liquid;
(2) utilize reacted solution in non-polar solven washing step (1), at 30 ~ 60 DEG C, then carry out water bath sonicator
Process, after having processed, the solution after recycling non-polar solven carrying out washing treatment, vacuum drying, obtain two sulfur of ferrum, manganese intercalation altogether
Change molybdenum nano material;
(3) the molybdenum disulfide nano material taking the prepared ferrum of 10 ~ 500 mg steps (2), manganese intercalation altogether joins 5 mL metatitanic acids
In four butyl esters, after stirring 1 hour, it is slowly added to 0.5 ~ 0.8 mL Fluohydric acid. while stirring, then instead at 160 ~ 200 DEG C
Answer in still and react 18 ~ 24 hours;
(4) by the product of step (3) gained, after ultra-pure water and dehydrated alcohol centrifuge washing three times, at 50 DEG C, vacuum is done
Dry, i.e. prepare FeMn-TiO2/MoS2;
Described n-butyllithium solution is the hexane solution of n-BuLi, and concentration is 1.6 mol/L;
Described iron salt is selected from one of following: iron sulfate, iron chloride, ferric nitrate, ferric acetate, organoiron compound;
Described manganese salt is selected from one of following: manganese sulfate, manganese chloride, manganese nitrate, manganese acetate, organo-manganese compound;
Described non-polar solven is selected from one of following: hexane, hexamethylene, carbon tetrachloride, benzene, toluene;
Described water bath sonicator processes, and the process time is 1 hour.
4. the preparation method of the electrochemistry Malathion sensor as described in claim 1 and 2, it is characterised in that described electricity
The concrete preparation process of chemistry Malathion sensor is:
(1) with ITO electro-conductive glass as working electrode, at the FeMn-TiO of electrode surface drop coating 8 ~ 12 L2/MoS2Colloidal sol, room temperature
Under dry;
(2) the electrode buffer solution PBS that will obtain in step (1), continues at electrode surface drop coating 8 ~ 12 L 10
The Malathion antibody-solutions of g/mL, preserves in 4 DEG C of refrigerators and dries;
(3) the electrode PBS that will obtain in step (2), continuing in electrode surface drop coating 8 ~ 12 L concentration is 100 g/
The bovine serum albumin solution of mL, preserves in 4 DEG C of refrigerators and dries;
(4) the electrode PBS that will obtain in step (3), preserves in 4 DEG C of refrigerators after drying, i.e. prepares electrochemistry horse
Draw sulfur phosphorus sensor;
Described FeMn-TiO2/MoS2Colloidal sol is by the FeMn-TiO of 50 mg2/MoS2Powder is dissolved in 10 mL ultra-pure waters, and
The hydrosol prepared after ultrasonic 30 min;
Described PBS is the phosphate buffered solution of 10 mmol/L, and the pH value of described phosphate buffered solution is 7.4.
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