CN105738437B - A kind of preparation method and application of the electrochemistry parathion sensor based on metal and metal oxide co-doped nano composite - Google Patents
A kind of preparation method and application of the electrochemistry parathion sensor based on metal and metal oxide co-doped nano composite Download PDFInfo
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Abstract
The invention discloses a kind of preparation method of the electrochemistry parathion sensor based on metal and metal oxide co-doped nano composite.Belong to Nano-function thin films and biosensor technology field.The method comprises the steps of firstly, preparing a kind of New Two Dimensional nano material Co MoO3/TiO2@g‑C3N4Good biocompatibility and big specific surface area using the material, parathion antibody in load, then horseradish peroxidase ester is fixed by the crosslinked action of glutaraldehyde, when being detected, because horseradish peroxidase can be with catalyzing hydrogen peroxide, produce electrochemical signals, antibody is recycled to be combined the influence to electron transport ability with the specific quantification of antigen, so that current strength accordingly reduces, the electrochemica biological sensor that low cost, high sensitivity, specificity are good, detection is quick, prepare simple detection parathion has finally been made.
Description
Technical field
The present invention relates to a kind of preparation method of electrochemistry parathion sensor.Belong to Nano-function thin films and biology
Sensor technical field.
Background technology
Parathion is a kind of broad spectrum activity high poison insecticide, have tag, stomach toxicity, fumigation action, and plant can be infiltrated through
It is interior.Parathion is acted on insect quickly, available for fruit tree pest insects and wheat red mite etc. such as preventing and treating cotton, apple, citrus, pears, peaches.
In soil, parathion can slightly be migrated by the eluviation of water to deep subsoil.Parathion in soil can pass through plant
Thing root absorption and enter plant in.After people eat this kind of plant or plant containing its residue by mistake, parathion can pass through
Alimentary canal, respiratory tract and complete skin and mucous membrane enter human body, it may appear that Nausea and vomiting, have a headache, have loose bowels, whole body weakness nothing
The preliminary symptom of the poisonings such as power, long-term consumption or excessive eat can cause canceration.
At present, the method for detecting parathion mainly has chromatography, mass spectrography etc..Such method instrument is valuable, complex operation,
Laboratory personnel could be detected after needing professional training.Therefore, R＆D costs are low, detection is fast, high sensitivity, high specificity
Parathion sensor is significant.
Electrochemica biological sensor due to its high sensitivity, specificity it is good, easy to operate the advantages that be widely used in facing
The fields such as bed diagnosis, Pharmaceutical Analysis, environmental monitoring.More, its key is wherein studied with unmarked electrochemical immunosensor especially
Technology be to improve modified electrode to the fixed amount of antibody and to testing the signal response speed and size of bottom liquid.Titanium dioxide is
A kind of photocatalyst material being most widely used, simultaneously because good biocompatibility, is also commonly used as electrode matrix material.By
More high miller index surfaces can be exposed in sheet-like titanium dioxide nanomaterial, there is higher catalytic activity, titanium dioxide is received
Rice piece has than nano-particle preferably application prospect, and the research for titanium dioxide nanoplate also receives much concern.But dioxy
The sensitivity that change titanium poorly conductive also limit the electrochemical sensor built by single titanium dioxide nano material is not universal high,
It is unfavorable for practical application.Modification or compound special nano material, on the one hand add electrode ratio on semiconductor nano material
Surface area, strengthen electrodes conduct ability, on the other hand the two can produce concerted catalysis effect, and bigger enhancing is to hydrogen peroxide
Solution H2O2Catalytic response speed and current responsing signal size, greatly improve detection sensitivity.Therefore, design, prepare height
Effect, stable titanium dioxide nanoplate and its trim are the key technologies for preparing electrochemical sensor.
The content of the invention
Prepare that simple, high sensitivity, detection be quick, electrochemistry pair of high specificity it is an object of the invention to provide a kind of
The preparation method of sulphur phosphorus sensor, prepared sensor, quick, Sensitive Detection available for parathion.Based on this purpose, sheet
Invention is prepared for a kind of New Two Dimensional nano composite material first, i.e., molybdenum oxide/dioxy of In-situ reaction cobalt doped on carbonitride
Change titanium nanometer sheet Co-MoO3/TiO2@g-C3N4, good biocompatibility and big specific surface area using the material, load
Upper parathion antibody, horseradish peroxidase ester is then fixed by the crosslinked action of glutaraldehyde, when being detected, due to horseradish
Peroxidase can produce electrochemical signals with catalyzing hydrogen peroxide, recycle antibody to be combined with the specific quantification of antigen pair
The influence of electron transport ability so that current strength accordingly reduces, and finally realizes and is detected using unmarked electrochemical method
The structure of the biology sensor of parathion.
The technical solution adopted by the present invention is as follows：
A kind of 1. system of the electrochemistry parathion sensor based on metal and metal oxide co-doped nano composite
Preparation Method, described metal and metal oxide co-doped nano composite are the oxidation of In-situ reaction cobalt doped on carbonitride
The two-dimensional nano composite Co-MoO of molybdenum/titanium dioxide nanoplate3/TiO2@g-C3N4；Described electrochemistry parathion sensing
Device is by working electrode, Co-MoO3/TiO2@g-C3N4, parathion antibody, bovine serum albumin(BSA), glutaraldehyde, horseradish peroxidase
Composition；
Characterized in that, described preparation method includes following preparation process：
a. Co-MoO3/TiO2@g-C3N4Preparation；
B. the preparation of electrochemistry parathion sensor；
Wherein, step a prepares Co-MoO3/TiO2@g-C3N4Concretely comprise the following steps：
First, take 0.6 ~ 1.0 mmol sodium molybdates and 0.8 ~ 1.2 mmol cobalt salts to be added in 5 mL butyl titanates, stir
During mixing, 0.5 ~ 0.8 mL hydrofluoric acid is slowly added to, reacts 18 ~ 24 hours, is cooled in a kettle at 160 ~ 200 DEG C
After room temperature, with ultra-pure water and absolute ethyl alcohol centrifuge washing three times after, be dried in vacuo at 50 DEG C；Secondly, 150 ~ 250 mg are taken to dry
Solid afterwards mixes with 400 mg melamines, and grind into powder；Then, the powder of grinding is put into Muffle furnace, heated up
Speed is 1 ~ 3 DEG C/min, is calcined 0.5 ~ 5 hour at 480 ~ 560 DEG C；Finally, the powder after calcining is cooled to room temperature, i.e.,
Co-MoO is made3/TiO2@g-C3N4；
Described cobalt salt is selected from one of following：Cobaltous sulfate, cobalt chloride, cobalt nitrate；
Step b prepares concretely comprising the following steps for electrochemistry parathion sensor：
（1）Using glass-carbon electrode as working electrode, in the μ L of electrode surface drop coating 8 ~ 12 Co-MoO3/TiO2@g-C3N4It is molten
Glue, dry at room temperature；
（2）By step（1）In obtained electrode cushioning liquid PBS, continue in the μ L of electrode surface drop coating 8 ~ 12
10 μ g/mL parathion antibody-solutions, preserve in 4 DEG C of refrigerators and dry；
（3）By step（2）In obtained electrode PBS, it is 100 to continue in the μ L concentration of electrode surface drop coating 8 ~ 12
μ g/mL bovine serum albumin solution, preserve in 4 DEG C of refrigerators and dry；
（4）By step（3）In obtained electrode PBS, the glutaraldehyde continued in the μ L of electrode surface drop coating 2 ~ 4 is molten
Liquid, preserve in 4 DEG C of refrigerators and dry；
（5）By step（4）In obtained electrode PBS, it is 20 μ to continue in the μ L concentration of electrode surface drop coating 6 ~ 10
G/mL horseradish peroxidase solution, preserve in 4 DEG C of refrigerators and dry；
（6）By step（5）In obtained electrode PBS, preserved in 4 DEG C of refrigerators after drying, that is, electrification be made
Learn parathion sensor；
Described Co-MoO3/TiO2@g-C3N4Colloidal sol is by 50 mg Co-MoO3/TiO2@g-C3N4Powder is dissolved in 10
In mL ultra-pure waters, and the obtained hydrosol after 30 min of ultrasound；
Described PBS is 10mmol/L phosphate buffer solution, and the pH value of described phosphate buffer solution is 7.4；
Described glutaraldehyde solution is the glutaraldehyde water solution that volume ratio is 2.5%.
2. the application of the electrochemistry parathion sensor prepared by preparation method of the present invention, it is characterised in that bag
Include following applying step：
A. standard liquid is prepared：Prepare the parathion standard liquid of one group of various concentrations including blank standard specimen；
B. working electrode is modified：It is by the electrochemistry parathion sensor prepared by preparation method as described in the present invention
Working electrode, the parathion standard liquids of the various concentrations prepared in step a is distinguished into drop coating to working electrode surface, 4 DEG C
Preserved in refrigerator；
C. working curve is drawn：Using saturated calomel electrode electrode as reference electrode, platinum electrode is used as to electrode, with
The working electrode composition three-electrode system that step b has been modified, connects electrochemical workstation, successively adds 15 in a cell
ML PBS and the mol/L of 20 μ L 5 H2O2；By the working electrode of chronoamperometry detection assembling to H2O2Response；Blank
The response current of standard specimen is designated asI 0, the response current of the parathion standard liquid containing various concentrations is denoted asI i, response current reduction
Difference be ΔI = I 0-I i, ΔIWith the mass concentration of parathion standard liquidCBetween it is linear, draw ΔI-CWork
Make curve；
D. the detection of parathion：The parathion standard liquid in step a is replaced with testing sample, according in step b and c
Method detected, according to the difference DELTA of response current intensity decreasesIAnd working curve, obtain parathion in testing sample
Content.
The useful achievement of the present invention
（1）Electrochemistry parathion sensor of the present invention is prepared simply, easy to operate, is realized to the fast of sample
Fast, sensitive, high selectivity detection, and cost is low, can be applied to portable inspectiont, has market development prospect；
（2）The present invention is prepared for New Two Dimensional nano material Co-MoO first3/TiO2@g-C3N4, due to cobalt molybdenum oxide/
Growth in situ on titanium dioxide nanoplate and fully contacted with titanium dioxide nanoplate, utilize the metal surface plasma body of cobalt
Effect and molybdenum oxide and the mutual promoting action both titanium dioxide, effectively increase semiconductor substrate electron transmission ability and
Catalytic activity, although solving, titanium dioxide nanoplate specific surface area is bigger and mesoporous characteristic is applied to electrochemical-based material
Material, but the technical problem that electro-chemical activity is not high and current signal is unstable；Simultaneously because carbonitride g-C3N4Good lead
Electrically and electrochemistry characteristic, along with titanium dioxide nanoplate is fully dispersed thereon, greatly increase electron transmission
Ability, solves titanium dioxide nanoplate poorly conductive and current responsing signal is weak and be unfavorable for preparing the skill of electrochemical sensor
Art problem, therefore, effective preparation of the material, there is important scientific meaning and application value；
（3）The present invention is first by Co-MoO3/TiO2@g-C3N4Applied in the preparation of electrochemica biological sensor, significantly
The strength and stability of current signal is improved, substantially increases the detection sensitivity of electrochemical sensor so that electrochemical student
Thing sensor realizes the application in real work；The application of the material, also it is associated biomolecule sensor, as optical electro-chemistry passes
Sensor, Electrochemiluminescsensor sensor etc. provide Technical Reference, have extensive potential use value.
Embodiment
The Co-MoO of embodiment 13/TiO2@g-C3N4Preparation
First, 0.6 mmol sodium molybdates and 0.8 mmol cobalt salts is taken to be added in 5 mL butyl titanates, in whipping process,
0.5 mL hydrofluoric acid is slowly added to, is reacted in a kettle at 160 DEG C 24 hours, after being cooled to room temperature, with ultra-pure water and anhydrous
Ethanol centrifuge washing three times after, be dried in vacuo at 50 DEG C；Secondly, the dried solids of 150 mg are taken to be mixed with 400 mg melamines
Close, and grind into powder；Then, the powder of grinding is put into Muffle furnace, programming rate is 1 DEG C/min, at 480 DEG C
Calcining 5 hours；Finally, the powder after calcining is cooled to room temperature, that is, Co-MoO is made3/TiO2@g-C3N4；
Described cobalt salt is cobaltous sulfate.
The Co-MoO of embodiment 23/TiO2@g-C3N4Preparation
First, 0.8 mmol sodium molybdates and 1.0 mmol cobalt salts are taken to be added in 5 mL butyl titanates, in whipping process,
0.65 mL hydrofluoric acid is slowly added to, is reacted in a kettle at 180 DEG C 21 hours, after being cooled to room temperature, with ultra-pure water and nothing
Water-ethanol centrifuge washing three times after, be dried in vacuo at 50 DEG C；Secondly, the dried solids of 200 mg and 400 mg melamines are taken
Mixing, and grind into powder；Then, the powder of grinding is put into Muffle furnace, programming rate is 2 DEG C/min, at 520 DEG C
Lower calcining 2 hours；Finally, the powder after calcining is cooled to room temperature, that is, Co-MoO is made3/TiO2@g-C3N4；
Described cobalt salt is cobalt chloride.
The Co-MoO of embodiment 33/TiO2@g-C3N4Preparation
First, 1.0 mmol sodium molybdates and 1.2 mmol cobalt salts are taken to be added in 5 mL butyl titanates, in whipping process,
0.8 mL hydrofluoric acid is slowly added to, is reacted in a kettle at 200 DEG C 18 hours, after being cooled to room temperature, with ultra-pure water and nothing
Water-ethanol centrifuge washing three times after, be dried in vacuo at 50 DEG C；Secondly, the dried solids of 250 mg and 400 mg melamines are taken
Mixing, and grind into powder；Then, the powder of grinding is put into Muffle furnace, programming rate is 3 DEG C/min, at 560 DEG C
Calcining 0.5 hour；Finally, the powder after calcining is cooled to room temperature, that is, Co-MoO is made3/TiO2@g-C3N4；
Described cobalt salt is cobalt nitrate.
The preparation method of the electrochemistry parathion sensor of embodiment 4
（1）Using a width of 1 cm, a length of 4 cm glass-carbon electrode as working electrode, in the μ L of electrode surface drop coating 8 Co-
MoO3/TiO2@g-C3N4Colloidal sol, dry at room temperature；
（2）By step（1）In obtained electrode cushioning liquid PBS, continue in the μ of 8 μ L of electrode surface drop coating 10
G/mL parathion antibody-solutions, preserve in 4 DEG C of refrigerators and dry；
（3）By step（2）In obtained electrode PBS, it is 100 μ to continue in the μ L concentration of electrode surface drop coating 8
G/mL bovine serum albumin solution, preserve in 4 DEG C of refrigerators and dry；
（4）By step（3）In obtained electrode PBS, the glutaraldehyde continued in the μ L of electrode surface drop coating 2 is molten
Liquid, preserve in 4 DEG C of refrigerators and dry；
（5）By step（4）In obtained electrode PBS, it is 20 μ g/ to continue in the μ L concentration of electrode surface drop coating 6
ML horseradish peroxidase ester solution, preserve in 4 DEG C of refrigerators and dry；
（6）By step（5）In obtained electrode PBS, preserved in 4 DEG C of refrigerators after drying, that is, electrification be made
Learn parathion sensor；
Described Co-MoO3/TiO2@g-C3N4Colloidal sol is by 50 mg Co-MoO3/TiO2@g-C3N4Powder is dissolved in 10
In mL ultra-pure waters, and the obtained hydrosol after 30 min of ultrasound；
Described PBS is 10mmol/L phosphate buffer solution, and the pH value of described phosphate buffer solution is 7.4；
Described glutaraldehyde solution is the glutaraldehyde water solution that volume ratio is 2.5%.
The preparation method of the electrochemistry parathion sensor of embodiment 5
（1）Using a width of 1 cm, a length of 4 cm glass-carbon electrode as working electrode, in the μ L of electrode surface drop coating 10 Co-
MoO3/TiO2@g-C3N4Colloidal sol, dry at room temperature；
（2）By step（1）In obtained electrode cushioning liquid PBS, continue in the μ L 10 of electrode surface drop coating 10
μ g/mL parathion antibody-solutions, preserve in 4 DEG C of refrigerators and dry；
（3）By step（2）In obtained electrode PBS, it is 100 μ to continue in the μ L concentration of electrode surface drop coating 10
G/mL bovine serum albumin solution, preserve in 4 DEG C of refrigerators and dry；
（4）By step（3）In obtained electrode PBS, the glutaraldehyde continued in the μ L of electrode surface drop coating 3 is molten
Liquid, preserve in 4 DEG C of refrigerators and dry；
（5）By step（4）In obtained electrode PBS, it is 20 μ g/ to continue in the μ L concentration of electrode surface drop coating 8
ML horseradish peroxidase ester solution, preserve in 4 DEG C of refrigerators and dry；
（6）By step（5）In obtained electrode PBS, preserved in 4 DEG C of refrigerators after drying, that is, electrification be made
Learn parathion sensor；
Described Co-MoO3/TiO2@g-C3N4Colloidal sol is by 50 mg Co-MoO3/TiO2@g-C3N4Powder is dissolved in 10
In mL ultra-pure waters, and the obtained hydrosol after 30 min of ultrasound；
Described PBS is 10 mmol/L phosphate buffer solution, and the pH value of described phosphate buffer solution is 7.4；
Described glutaraldehyde solution is the glutaraldehyde water solution that volume ratio is 2.5%.
The preparation method of the electrochemistry parathion sensor of embodiment 6
（1）Using a width of 1 cm, a length of 4 cm glass-carbon electrode as working electrode, in the μ L of electrode surface drop coating 12 Co-
MoO3/TiO2@g-C3N4Colloidal sol, dry at room temperature；
（2）By step（1）In obtained electrode cushioning liquid PBS, continue in the μ L 10 of electrode surface drop coating 12
μ g/mL parathion antibody-solutions, preserve in 4 DEG C of refrigerators and dry；
（3）By step（2）In obtained electrode PBS, it is 100 μ to continue in the μ L concentration of electrode surface drop coating 12
G/mL bovine serum albumin solution, preserve in 4 DEG C of refrigerators and dry；
（4）By step（3）In obtained electrode PBS, the glutaraldehyde continued in the μ L of electrode surface drop coating 4 is molten
Liquid, preserve in 4 DEG C of refrigerators and dry；
（5）By step（4）In obtained electrode PBS, it is 20 μ g/ to continue in the μ L concentration of electrode surface drop coating 10
ML horseradish peroxidase ester solution, preserve in 4 DEG C of refrigerators and dry；
（6）By step（5）In obtained electrode PBS, preserved in 4 DEG C of refrigerators after drying, that is, electrification be made
Learn parathion sensor；
Described Co-MoO3/TiO2@g-C3N4Colloidal sol is by 50 mg Co-MoO3/TiO2@g-C3N4Powder is dissolved in 10
In mL ultra-pure waters, and the obtained hydrosol after 30 min of ultrasound；
Described PBS is 10 mmol/L phosphate buffer solution, and the pH value of described phosphate buffer solution is 7.4；
Described glutaraldehyde solution is the glutaraldehyde water solution that volume ratio is 2.5%.
Electrochemistry parathion sensor prepared by the embodiment 1 ~ 6 of embodiment 7, applied to the detection of parathion, step is such as
Under：
（1）Standard liquid is prepared：Prepare the parathion standard liquid of one group of various concentrations including blank standard specimen；
（2）Working electrode is modified：It is by the electrochemistry parathion sensor prepared by preparation method as described in the present invention
Working electrode, by step（1）The parathion standard liquids of the various concentrations of middle preparation distinguishes drop coating to working electrode surface, 4 DEG C
Preserved in refrigerator；
（3）Working curve is drawn：Using saturated calomel electrode electrode as reference electrode, platinum electrode is used as to electrode, with
The working electrode composition three-electrode system that step b has been modified, connects electrochemical workstation, successively adds 15 in a cell
ML PBS and the mol/L of 20 μ L 5 H2O2；By the working electrode of chronoamperometry detection assembling to H2O2Response；Blank
The response current of standard specimen is designated asI 0, the response current of the parathion standard liquid containing various concentrations is denoted asI i, response current reduction
Difference be ΔI = I 0-I i, ΔIWith the mass concentration of parathion standard liquidCBetween it is linear, draw ΔI-CWork
Make curve；The linear detection range of parathion is：0.009 ~ 200 ng/mL, detection are limited to：3.0 pg/mL；
（4）The detection of parathion：Step is replaced with testing sample（1）In parathion standard liquid, according to step（2）With
（3）In method detected, according to the difference DELTA of response current intensity decreasesIAnd working curve, it is right in testing sample to obtain
The content of sulphur phosphorus.
Claims (2)
- A kind of 1. preparation side of the electrochemistry parathion sensor based on metal and metal oxide co-doped nano composite Method, described metal and metal oxide co-doped nano composite be carbonitride on In-situ reaction cobalt doped molybdenum oxide/ The two-dimensional nano composite Co-MoO of titanium dioxide nanoplate3/TiO2@g-C3N4；Described electrochemistry parathion sensor by Working electrode, Co-MoO3/TiO2@g-C3N4, parathion antibody, bovine serum albumin(BSA), glutaraldehyde, horseradish peroxidase group Into；Characterized in that, described preparation method includes following preparation process：a. Co-MoO3/TiO2@g-C3N4Preparation；B. the preparation of electrochemistry parathion sensor；Wherein, step a prepares Co-MoO3/TiO2@g-C3N4Concretely comprise the following steps：First, 0.6 ~ 1.0 mmol sodium molybdates and 0.8 ~ 1.2 mmol cobalt salts is taken to be added in 5 mL butyl titanates, it is stirred Cheng Zhong, 0.5 ~ 0.8 mL hydrofluoric acid is slowly added to, is reacted in a kettle at 160 ~ 200 DEG C 18 ~ 24 hours, is cooled to room temperature Afterwards, with ultra-pure water and absolute ethyl alcohol centrifuge washing three times after, be dried in vacuo at 50 DEG C；Secondly, take 150 ~ 250 mg dried Solid mixes with 400 mg melamines, and grind into powder；Then, the powder of grinding is put into Muffle furnace, programming rate For 1 ~ 3 DEG C/min, calcined 0.5 ~ 5 hour at 480 ~ 560 DEG C；Finally, the powder after calcining is cooled to room temperature, that is, be made Co-MoO3/TiO2@g-C3N4；Described cobalt salt is selected from one of following：Cobaltous sulfate, cobalt chloride, cobalt nitrate；Step b prepares concretely comprising the following steps for electrochemistry parathion sensor：（1）Using glass-carbon electrode as working electrode, in the μ L of electrode surface drop coating 8 ~ 12 Co-MoO3/TiO2@g-C3N4Colloidal sol, room temperature Under dry；（2）By step（1）In obtained electrode cushioning liquid PBS, continue in the μ of 8 ~ 12 μ L of electrode surface drop coating 10 G/mL parathion antibody-solutions, preserve in 4 DEG C of refrigerators and dry；（3）By step（2）In obtained electrode PBS, it is 100 μ g/ to continue in the μ L concentration of electrode surface drop coating 8 ~ 12 ML bovine serum albumin solution, preserve in 4 DEG C of refrigerators and dry；（4）By step（3）In obtained electrode PBS, continue the glutaraldehyde solution in the μ L of electrode surface drop coating 2 ~ 4,4 Preserve and dry in DEG C refrigerator；（5）By step（4）In obtained electrode PBS, it is 20 μ g/mL to continue in the μ L concentration of electrode surface drop coating 6 ~ 10 Horseradish peroxidase solution, preserve in 4 DEG C of refrigerators and dry；（6）By step（5）In obtained electrode PBS, preserved in 4 DEG C of refrigerators after drying, that is, electrochemistry pair be made Sulphur phosphorus sensor；Described Co-MoO3/TiO2@g-C3N4Colloidal sol is by 50 mg Co-MoO3/TiO2@g-C3N4Powder is dissolved in 10 mL and surpassed In pure water, and the obtained hydrosol after 30 min of ultrasound；Described PBS is 10mmol/L phosphate buffer solution, and the pH value of described phosphate buffer solution is 7.4；Described glutaraldehyde solution is the glutaraldehyde water solution that volume ratio is 2.5%.
- 2. the application of the electrochemistry parathion sensor prepared by preparation method as claimed in claim 1, it is characterised in that bag Include following applying step：A. standard liquid is prepared：Prepare the parathion standard liquid of one group of various concentrations including blank standard specimen；B. working electrode is modified：It is work by the electrochemistry parathion sensor prepared by preparation method as claimed in claim 1 Make electrode, the parathion standard liquid for the various concentrations prepared in step a is distinguished into drop coating to working electrode surface, 4 DEG C of ice Preserved in case；C. working curve is drawn：Using saturated calomel electrode electrode as reference electrode, platinum electrode is used as to electrode, with step b The working electrode composition three-electrode system modified, connects electrochemical workstation, successively adds 15 mL PBS in a cell With the mol/L of 20 μ L 5 H2O2；By the working electrode of chronoamperometry detection assembling to H2O2Response；Blank standard specimen Response current is designated asI 0, the response current of the parathion standard liquid containing various concentrations is denoted asI i, the difference of response current reduction For ΔI = I 0-I i, ΔIWith the mass concentration of parathion standard liquidCBetween it is linear, draw ΔI-CWorking curve；D. the detection of parathion：The parathion standard liquid in step a is replaced with testing sample, according to the side in step b and c Method is detected, according to the difference DELTA of response current intensity decreasesIAnd working curve, obtain containing for parathion in testing sample Amount.
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| CN108585044B (en) * | 2018-05-21 | 2021-01-19 | 济南大学 | Co-MoO with mylikes structure2Simple preparation and electrocatalysis application of nanosphere |
| CN109254046B (en) * | 2018-11-05 | 2021-01-12 | 济南大学 | Nitrofuran antibiotic sensor and preparation method thereof |
| CN109254045B (en) * | 2018-11-05 | 2021-01-12 | 济南大学 | Cobalt-based nitride sensor for detecting praziquantel and preparation method thereof |
| CN114720525B (en) * | 2022-03-22 | 2024-01-05 | 华南农业大学 | Parathion immunosensor and preparation method and application thereof |
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| JPH07100603B2 (en) * | 1987-08-17 | 1995-11-01 | 住友電気工業株式会社 | Composite carbonitride manufacturing method |
| CN103675075A (en) * | 2013-11-27 | 2014-03-26 | 复旦大学 | Organophosphorus detection method based on microelectrode chip |
| CN104777201B (en) * | 2015-04-16 | 2017-07-11 | 湖北民族学院 | A kind of preparation method for the electrochemical sensor for detecting parathion |
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