CN105699645B - A kind of preparation method and application of electrochemistry salbutamol sensor - Google Patents
A kind of preparation method and application of electrochemistry salbutamol sensor Download PDFInfo
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Abstract
The invention discloses a kind of preparation method of electrochemistry salbutamol sensor.Belong to Nano-function thin films and biosensor technology field.The method comprises the steps of firstly, preparing a kind of novel dual metal codope two-dimension nano materials FeMn TiO2/g‑C3N4Using the good biocompatibility and big specific surface area of the material, salbutamol antibody in load, then by the crosslinked action horseradish peroxidase of glutaraldehyde, when being detected, because horseradish peroxidase can be with catalyzing hydrogen peroxide, produce electrochemical signals, recycle antibody that the influence to electron transport ability is combined with the specific quantification of antigen, so that current strength is accordingly reduced, low cost is finally obtained, sensitivity is high, specificity is good, detection is quick, prepare the electrochemica biological sensor of simple detection salbutamol.
Description
Technical field
The present invention relates to a kind of preparation method of electrochemistry salbutamol sensor.Belong to Nano-function thin films and life
Thing sensor technical field.
Background technology
Salbutamol is selective β 2 receptor agonist, is used as clenbuterol hydrochloride in the cultivation of modern pork to improve live pig
Lean meat yield.Begin within 2002, be listed in cultivation industry forbidden drug, must not be added in livestock and poultry cultivation.
At present, the method for detection salbutamol mainly has chromatography, mass spectrography etc..Such method instrument is valuable, operation is multiple
Miscellaneous, laboratory personnel can just be detected after needing professional training.Therefore, R＆D costs are low, detection is fast, sensitivity is high, specificity
Strong salbutamol sensor is significant.
Electrochemica biological sensor the advantages of its sensitivity is high, specificity is good, easy to operate due to being widely used in facing
The fields such as bed diagnosis, Pharmaceutical Analysis, environmental monitoring.Wherein study more with unmarked electrochemical immunosensor especially, it is crucial
Technology be improve modified electrode antagonist fixed amount and to test bottom liquid signal response speed and size.Titanium dioxide is
A kind of photocatalyst material being most widely used, simultaneously because good biocompatibility, is also commonly used as electrode matrix material.By
More high miller index surfaces can be exposed in sheet-like titanium dioxide nanomaterial, with catalysis activity higher, titanium dioxide is received
Rice piece has than nano-particle preferably application prospect, and the research for titanium dioxide nanoplate also receives much concern.But, dioxy
The sensitivity that change titanium poorly conductive also limit the electrochemical sensor built by single titanium dioxide nano material is not universal high,
It is unfavorable for practical application.Modified on semiconductor nano material or compound special nano material, on the one hand increased electrode ratio
Surface area, strengthens electrodes conduct ability, and on the other hand the two can produce concerted catalysis to act on, and bigger enhancing is to hydrogen peroxide
Solution H2O2Catalytic response speed and current responsing signal size, greatly improve detection sensitivity.Therefore, design, prepare height
Effect, the titanium dioxide nanoplate of stabilization and its trim are the key technologies for preparing electrochemical sensor.
The content of the invention
Prepare that simple, sensitivity is high, detection is quick, high specificity electrochemistry sand it is an object of the invention to provide a kind of
The preparation method of butylamine alcohol sensor, prepared sensor can be used for quick, the Sensitive Detection of salbutamol.Based on this mesh
, the method comprises the steps of firstly, preparing a kind of bimetallic codope two-dimension nano materials, i.e. iron and the titanium dioxide of manganese bimetallic codope
Titanium nanometer sheet In-situ reaction carbonitride two-dimensional nano composite FeMn-TiO2/g-C3N4, using the good biology of the material
Then compatibility and big specific surface area, salbutamol antibody in load fix horseradish peroxide by the crosslinked action of glutaraldehyde
Compound enzyme, when being detected, because horseradish peroxidase can produce electrochemical signals with catalyzing hydrogen peroxide, recycles
Antibody is combined the influence to electron transport ability with the specific quantification of antigen so that current strength is accordingly reduced, final to realize
Using the structure of the biology sensor of unmarked electrochemical method detection salbutamol.
The technical solution adopted by the present invention is as follows：
1. a kind of preparation method of the electrochemistry salbutamol sensor based on magnetic two-dimensional nano composite, described
Magnetic two-dimensional nano composite be the titanium dioxide nanoplate In-situ reaction carbonitride two dimension of iron and manganese bimetallic codope
Nano composite material FeMn-TiO2/g-C3N4, described electrochemistry salbutamol sensor is by working electrode, FeMn-TiO2/g-
C3N4, salbutamol antibody, bovine serum albumin(BSA), glutaraldehyde, horseradish peroxidase composition；
Characterized in that, described preparation method includes following preparation process：
a. FeMn-TiO2/g-C3N4Preparation；
B. the preparation of electrochemistry salbutamol sensor；
Wherein, step a prepares FeMn-TiO2/g-C3N4Concretely comprise the following steps：
First, take 0.8 mmol molysite and 0.8 ~ 1.2 mmol manganese salts are added in 5 mL butyl titanates, whipping process
In, 0.5 ~ 0.8 mL hydrofluoric acid is slowly added to, reacted in a kettle. at 160 ~ 200 DEG C 18 ~ 24 hours, after being cooled to room temperature,
After with ultra-pure water and absolute ethyl alcohol centrifuge washing three times, it is vacuum dried at 50 DEG C；Secondly, the dried solids of 150 ~ 250 mg are taken
Mix with 400 mg melamines, and grind into powder；Then, the powder of grinding is put into Muffle furnace, programming rate is 1 ~ 3
DEG C/min, calcined 0.5 ~ 5 hour at 480 ~ 560 DEG C；Finally, the powder after calcining is cooled to room temperature, that is, FeMn- is obtained
TiO2/g-C3N4；
Described molysite is selected from one of following：Ferric sulfate, iron chloride, ferric nitrate；
Described manganese salt is selected from one of following：Manganese sulfate, manganese chloride, manganese nitrate；
Step b prepares concretely comprising the following steps for electrochemistry salbutamol sensor：
（1）With glass-carbon electrode as working electrode, in the FeMn-TiO of the μ L of electrode surface drop coating 8 ~ 122/g-C3N4Colloidal sol, room
Dried under temperature；
（2）By step（1）In the electrode cushioning liquid PBS that obtains, continue in the μ L of electrode surface drop coating 8 ~ 12
The salbutamol antibody-solutions of 10 μ g/mL, preserve in 4 DEG C of refrigerators and dry；
（3）By step（2）In the electrode PBS that obtains, it is 100 to continue in the μ L concentration of electrode surface drop coating 8 ~ 12
The bovine serum albumin solution of μ g/mL, preserves in 4 DEG C of refrigerators and dries；
（4）By step（3）In the electrode PBS that obtains, continue molten in the glutaraldehyde of the μ L of electrode surface drop coating 2 ~ 4
Liquid, preserves in 4 DEG C of refrigerators and dries；
（5）By step（4）In the electrode PBS that obtains, it is 20 μ to continue in the μ L concentration of electrode surface drop coating 6 ~ 10
The horseradish peroxidase solution of g/mL, preserves in 4 DEG C of refrigerators and dries；
（6）By step（5）In the electrode PBS that obtains, preserved in 4 DEG C of refrigerators after drying, that is, electrification is obtained
Learn salbutamol sensor；
Described FeMn-TiO2/g-C3N4Solution is by the FeMn-TiO of 50 mg2/g-C3N4It is ultrapure that powder is dissolved in 10 mL
In water, and the obtained hydrosol after ultrasonic 30 min；
Described PBS is the PBS of 10mmol/L, and the pH value of described PBS is 7.4；
Described glutaraldehyde solution is glutaraldehyde water solution that volume ratio is 2.5%.
2. the application of the electrochemistry salbutamol sensor prepared by preparation method of the present invention, it is characterised in that
Including following applying step：
A. standard liquid is prepared：Prepare one group of salbutamol standard liquid including the various concentrations including blank standard specimen；
B. working electrode modification：By the electrochemistry salbutamol sensing prepared by preparation method as claimed in claim 1
Device is working electrode, and the salbutamol standard liquid of the various concentrations that will be prepared in step a distinguishes drop coating to working electrode surface,
Preserved in 4 DEG C of refrigerators；
C. working curve is drawn：Using Ag/AgCl electrodes as reference electrode, platinum electrode as to electrode, with step b
The working electrode composition three-electrode system modified, is connected in electrochemistry detecting apparatus；15 are successively added in a cell
The K of mL2S2O8The H of solution and 100 μ L2O2Solution；Cyclical voltage is applied to the working electrode for assembling with cyclic voltammetry；According to
Relation between the light signal strength and salbutamol antigen concentration of standard solution of the electrochemistry of gained, drawing curve；It is empty
The light signal strength of white standard specimen is designated asD 0, the light signal strength of the salbutamol standard liquid containing various concentrations is designated asD i, response
The difference of light signal strength reduction is ΔD = D 0-D i, ΔDWith the mass concentration of salbutamol standard liquidCBetween linear pass
System, draws ΔD-CWorking curve；
D. the detection of salbutamol：Replace the salbutamol standard liquid in step a with testing sample, according to step b and
Method in c is detected, according to the difference DELTA of response light signal strength reductionDAnd working curve, obtain sand in testing sample
The content of butylamine alcohol；
Described K2S2O8Solution is by 1 mol K2S2O8It is dissolved in the pH=6.5 cushioning liquid of 10 L with 1 mol KCl and is made
Standby to obtain, described pH=6.5 cushioning liquid is PBS that pH value is 6.5；
Described H2O2Solution is aqueous hydrogen peroxide solution, and the concentration of described aqueous hydrogen peroxide solution is 10%.
Useful achievement of the invention
（1）Electrochemistry salbutamol sensor of the present invention is prepared simply, easy to operate, is realized to the fast of sample
Fast, sensitive, high selectivity detection, and low cost, can be applied to portable inspectiont, with market development prospect；
（2）The present invention is prepared for new light-sensitive material FeMn-TiO first2/g-C3N4, because iron, manganese are received in titanium dioxide
Rice piece on growth in situ and fully contacted with titanium dioxide nanoplate, using iron, manganese metal surface plasma body effect with
And the mutual promoting action of the two, semiconductor substrate electron transmission ability and catalysis activity are effectively increased, solve titanium dioxide
Although titanium nanometer sheet specific surface area than larger and mesoporous characteristic be applied to electrochemical-based material, electro-chemical activity it is not high and
The unstable technical problem of current signal；Simultaneously because carbonitride g-C3N4Good electric conductivity and electrochemistry characteristic, then add
Upper titanium dioxide nanoplate thereon fully dispersed, greatly increases electron transmission ability, solves nano titania
Piece poorly conductive and current responsing signal be weak and be unfavorable for preparing the technical problem of electrochemical sensor, therefore, the material has
Prepared by effect, with important scientific meaning and application value；
（3）The present invention is first by FeMn-TiO2/g-C3N4It is applied in the preparation of electrochemica biological sensor, significantly improves
The strength and stability of current signal, substantially increases the detection sensitivity of electrochemical sensor so that electrochemica biological is passed
Sensor realizes the application in real work；The application of the material, is also associated biomolecule sensor, such as Optical Electro-Chemistry sensing
Device, Electrochemiluminescsensor sensor etc. provide Technical Reference, with extensive potential use value.
Specific embodiment
The FeMn-TiO of embodiment 12/g-C3N4Preparation
First, take 0.8 mmol molysite and 0.8 mmol manganese salts are added in 5 mL butyl titanates, in whipping process, delay
It is slow to add 0.5 mL hydrofluoric acid, reacted in a kettle. at 160 DEG C 24 hours, after being cooled to room temperature, with ultra-pure water and anhydrous second
After alcohol centrifuge washing three times, it is vacuum dried at 50 DEG C；Secondly, the dried solids of 150 mg are taken and 400 mg melamines is mixed
Close, and grind into powder；Then, the powder of grinding is put into Muffle furnace, programming rate is 1 DEG C/min, at 480 DEG C
Calcining 5 hours；Finally, the powder after calcining is cooled to room temperature, that is, FeMn-TiO is obtained2/g-C3N4；
Described molysite is ferric sulfate；
Described manganese salt is manganese sulfate.
The FeMn-TiO of embodiment 22/g-C3N4Preparation
First, take 0.8 mmol molysite and 1.0 mmol manganese salts are added in 5 mL butyl titanates, in whipping process, delay
It is slow to add 0.65 mL hydrofluoric acid, reacted in a kettle. at 180 DEG C 21 hours, after being cooled to room temperature, with ultra-pure water and anhydrous
After ethanol centrifuge washing three times, it is vacuum dried at 50 DEG C；Secondly, the dried solids of 200 mg are taken and 400 mg melamines is mixed
Close, and grind into powder；Then, the powder of grinding is put into Muffle furnace, programming rate is 2 DEG C/min, at 520 DEG C
Calcining 2 hours；Finally, the powder after calcining is cooled to room temperature, that is, FeMn-TiO is obtained2/g-C3N4；
Described molysite is iron chloride；
Described manganese salt is manganese chloride.
The FeMn-TiO of embodiment 32/g-C3N4Preparation
First, take 0.8 mmol molysite and 1.2 mmol manganese salts are added in 5 mL butyl titanates, in whipping process, delay
It is slow to add 0.8 mL hydrofluoric acid, reacted in a kettle. at 200 DEG C 18 hours, after being cooled to room temperature, with ultra-pure water and anhydrous
After ethanol centrifuge washing three times, it is vacuum dried at 50 DEG C；Secondly, the dried solids of 250 mg are taken and 400 mg melamines is mixed
Close, and grind into powder；Then, the powder of grinding is put into Muffle furnace, programming rate is 3 DEG C/min, is forged at 560 DEG C
Burn 0.5 hour；Finally, the powder after calcining is cooled to room temperature, that is, FeMn-TiO is obtained2/g-C3N4；
Described molysite is ferric nitrate；
Described manganese salt is manganese nitrate.
The preparation method of the electrochemistry salbutamol sensor of embodiment 4
（1）Using a width of 1 cm, a length of 4 cm glass-carbon electrode as working electrode, the μ L's of electrode surface drop coating 8
FeMn-TiO2/g-C3N4Colloidal sol, dries at room temperature；
（2）By step（1）In the electrode cushioning liquid PBS that obtains, continue in the μ of 8 μ L of electrode surface drop coating 10
The salbutamol antibody-solutions of g/mL, preserve in 4 DEG C of refrigerators and dry；
（3）By step（2）In the electrode PBS that obtains, it is 100 μ to continue in the μ L concentration of electrode surface drop coating 8
The bovine serum albumin solution of g/mL, preserves in 4 DEG C of refrigerators and dries；
（4）By step（3）In the electrode PBS that obtains, continue molten in the glutaraldehyde of the μ L of electrode surface drop coating 2
Liquid, preserves in 4 DEG C of refrigerators and dries；
（5）By step（4）In the electrode PBS that obtains, it is 20 μ g/ to continue in the μ L concentration of electrode surface drop coating 6
The horseradish peroxidase solution of mL, preserves in 4 DEG C of refrigerators and dries；
（6）By step（5）In the electrode PBS that obtains, preserved in 4 DEG C of refrigerators after drying, that is, electrification is obtained
Learn salbutamol sensor；
Described FeMn-TiO2/g-C3N4Colloidal sol is by the FeMn-TiO of 50 mg2/g-C3N4It is ultrapure that powder is dissolved in 10 mL
In water, and the obtained hydrosol after ultrasonic 30 min；
Described PBS is the PBS of 10mmol/L, and the pH value of described PBS is 7.4；
Described glutaraldehyde solution is glutaraldehyde water solution that volume ratio is 2.5%.
The preparation method of the electrochemistry salbutamol sensor of embodiment 5
（1）Using a width of 1 cm, a length of 4 cm glass-carbon electrode as working electrode, the μ L's of electrode surface drop coating 10
FeMn-TiO2/g-C3N4Colloidal sol, dries at room temperature；
（2）By step（1）In the electrode cushioning liquid PBS that obtains, continue in the μ L 10 of electrode surface drop coating 10
The salbutamol antibody-solutions of μ g/mL, preserve in 4 DEG C of refrigerators and dry；
（3）By step（2）In the electrode PBS that obtains, it is 100 μ to continue in the μ L concentration of electrode surface drop coating 10
The bovine serum albumin solution of g/mL, preserves in 4 DEG C of refrigerators and dries；
（4）By step（3）In the electrode PBS that obtains, continue molten in the glutaraldehyde of the μ L of electrode surface drop coating 3
Liquid, preserves in 4 DEG C of refrigerators and dries；
（5）By step（4）In the electrode PBS that obtains, it is 20 μ g/ to continue in the μ L concentration of electrode surface drop coating 8
The horseradish peroxidase solution of mL, preserves in 4 DEG C of refrigerators and dries；
（6）By step（5）In the electrode PBS that obtains, preserved in 4 DEG C of refrigerators after drying, that is, electrification is obtained
Learn salbutamol sensor；
Described FeMn-TiO2/g-C3N4Colloidal sol is by the FeMn-TiO of 50 mg2/g-C3N4It is ultrapure that powder is dissolved in 10 mL
In water, and the obtained hydrosol after ultrasonic 30 min；
Described PBS is the PBS of 10 mmol/L, and the pH value of described PBS is 7.4；
Described glutaraldehyde solution is glutaraldehyde water solution that volume ratio is 2.5%.
The preparation method of the electrochemistry salbutamol sensor of embodiment 6
（1）Using a width of 1 cm, a length of 4 cm glass-carbon electrode as working electrode, the μ L's of electrode surface drop coating 12
FeMn-TiO2/g-C3N4Colloidal sol, dries at room temperature；
（2）By step（1）In the electrode cushioning liquid PBS that obtains, continue in the μ L 10 of electrode surface drop coating 12
The salbutamol antibody-solutions of μ g/mL, preserve in 4 DEG C of refrigerators and dry；
（3）By step（2）In the electrode PBS that obtains, it is 100 μ to continue in the μ L concentration of electrode surface drop coating 12
The bovine serum albumin solution of g/mL, preserves in 4 DEG C of refrigerators and dries；
（4）By step（3）In the electrode PBS that obtains, continue molten in the glutaraldehyde of the μ L of electrode surface drop coating 4
Liquid, preserves in 4 DEG C of refrigerators and dries；
（5）By step（4）In the electrode PBS that obtains, it is 20 μ g/ to continue in the μ L concentration of electrode surface drop coating 10
The horseradish peroxidase solution of mL, preserves in 4 DEG C of refrigerators and dries；
（6）By step（5）In the electrode PBS that obtains, preserved in 4 DEG C of refrigerators after drying, that is, electrification is obtained
Learn salbutamol sensor；
Described FeMn-TiO2/g-C3N4Colloidal sol is by the FeMn-TiO of 50 mg2/g-C3N4It is ultrapure that powder is dissolved in 10 mL
In water, and the obtained hydrosol after ultrasonic 30 min；
Described PBS is the PBS of 10 mmol/L, and the pH value of described PBS is 7.4；
Described glutaraldehyde solution is glutaraldehyde water solution that volume ratio is 2.5%.
Electrochemistry salbutamol sensor prepared by 7 embodiment of embodiment 1 ~ 6, is applied to the detection of salbutamol, walks
It is rapid as follows：
（1）Standard liquid is prepared：Prepare one group of salbutamol standard liquid including the various concentrations including blank standard specimen；
（2）Working electrode is modified：By the electrochemistry salbutamol sensing prepared by preparation method as claimed in claim 1
Device is working electrode, by step（1）The salbutamol standard liquid of the various concentrations of middle preparation distinguishes drop coating to working electrode table
Face, preserves in 4 DEG C of refrigerators；
（3）Working curve is drawn：Using saturated calomel electrode electrode as reference electrode, platinum electrode as to electrode, with
The working electrode composition three-electrode system that step b is modified, connects electrochemical workstation, and 15 are successively added in a cell
The H of mL PBS and the mol/L of 20 μ L 52O2；Detect the working electrode of assembling to H by chronoamperometry2O2Response；Blank
The response current of standard specimen is designated asI 0, the response current of the salbutamol standard liquid containing various concentrations is denoted asI i, response current drop
Low difference is ΔI = I 0-I i, ΔIWith the mass concentration of salbutamol standard liquidCBetween it is linear, draw ΔI-CWorking curve；The linear detection range of salbutamol is：0.009 ~ 300 ng/mL, detection is limited to：3.0 pg/mL；
（4）The detection of salbutamol：Replace step with testing sample（1）In salbutamol standard liquid, according to step
（2）With（3）In method detected, according to response light signal strength reduction difference DELTAIAnd working curve, obtain treating test sample
The content of salbutamol in product.
Claims (2)
1. a kind of preparation method of electrochemistry salbutamol sensor, described electrochemistry salbutamol sensor is by work electricity
Pole, FeMn-TiO2/g-C3N4, salbutamol antibody, bovine serum albumin(BSA), glutaraldehyde, horseradish peroxidase composition；Described
FeMn-TiO2/g-C3N4For the titanium dioxide nanoplate In-situ reaction carbonitride two-dimensional nano of iron and manganese bimetallic codope is combined
Material；
Characterized in that, described preparation method includes following preparation process：
a. FeMn-TiO2/g-C3N4Preparation；
B. the preparation of electrochemistry salbutamol sensor；
Wherein, step a prepares FeMn-TiO2/g-C3N4Concretely comprise the following steps：
First, take 0.8 mmol molysite and 0.8 ~ 1.2 mmol manganese salts are added in 5 mL butyl titanates, in whipping process, delay
It is slow to add 0.5 ~ 0.8 mL hydrofluoric acid, reacted in a kettle. at 160 ~ 200 DEG C 18 ~ 24 hours, after being cooled to room temperature, with super
After pure water and absolute ethyl alcohol centrifuge washing three times, it is vacuum dried at 50 DEG C；Secondly, take the dried solids of 150 ~ 250 mg with
400 mg melamines mix, and grind into powder；Then, the powder of grinding is put into Muffle furnace, programming rate is 1 ~ 3
DEG C/min, calcined 0.5 ~ 5 hour at 480 ~ 560 DEG C；Finally, the powder after calcining is cooled to room temperature, that is, FeMn- is obtained
TiO2/g-C3N4；
Described molysite is selected from one of following：Ferric sulfate, iron chloride, ferric nitrate；
Described manganese salt is selected from one of following：Manganese sulfate, manganese chloride, manganese nitrate；
Step b prepares concretely comprising the following steps for electrochemistry salbutamol sensor：
（1）With glass-carbon electrode as working electrode, in the FeMn-TiO of the μ L of electrode surface drop coating 8 ~ 122/g-C3N4Colloidal sol, at room temperature
Dry；
（2）By step（1）In the electrode cushioning liquid PBS that obtains, continue in the μ of 8 ~ 12 μ L of electrode surface drop coating 10
The salbutamol antibody-solutions of g/mL, preserve in 4 DEG C of refrigerators and dry；
（3）By step（2）In the electrode PBS that obtains, it is 100 μ g/ to continue in the μ L concentration of electrode surface drop coating 8 ~ 12
The bovine serum albumin solution of mL, preserves in 4 DEG C of refrigerators and dries；
（4）By step（3）In the electrode PBS that obtains, continue the glutaraldehyde solution in the μ L of electrode surface drop coating 2 ~ 4,4 DEG C
Preserved in refrigerator and dried；
（5）By step（4）In the electrode PBS that obtains, it is 20 μ g/mL to continue in the μ L concentration of electrode surface drop coating 6 ~ 10
Horseradish peroxidase solution, preserved in 4 DEG C of refrigerators and dried；
（6）By step（5）In the electrode PBS that obtains, preserved in 4 DEG C of refrigerators after drying, that is, electrochemistry is obtained husky
Butylamine alcohol sensor；
Described FeMn-TiO2/g-C3N4Colloidal sol is by the FeMn-TiO of 50 mg2/g-C3N4Powder is dissolved in 10 mL ultra-pure waters
In, and the obtained hydrosol after ultrasonic 30 min；
Described PBS is the PBS of 10mmol/L, and the pH value of described PBS is 7.4；
Described glutaraldehyde solution is glutaraldehyde water solution that volume ratio is 2.5%.
2. the application of the electrochemistry salbutamol sensor prepared by preparation method as claimed in claim 1, it is characterised in that
Including following applying step：
A. standard liquid is prepared：Prepare one group of salbutamol standard liquid including the various concentrations including blank standard specimen；
B. working electrode modification：Electrochemistry salbutamol sensor prepared by preparation method as claimed in claim 1 is made
It is working electrode, the salbutamol standard liquid of the various concentrations that will be prepared in step a distinguishes drop coating to working electrode surface, 4
Preserved in DEG C refrigerator；
C. working curve is drawn：Saturated calomel electrode as reference electrode, platinum electrode are repaiied as to electrode with step b
The working electrode composition three-electrode system adornd, connects electrochemical workstation, and 15 mL PBS and 20 are successively added in a cell
The H of the mol/L of μ L 52O2；Detect the working electrode of assembling to H by chronoamperometry2O2Response；The response electricity of blank standard specimen
Stream is designated asI 0, the response current of the salbutamol standard liquid containing various concentrations is denoted asI i, the difference of response current reduction is ΔI = I 0-I i, ΔIWith the mass concentration of salbutamol standard liquidCBetween it is linear, draw ΔI-CWorking curve；
D. the detection of salbutamol：Replace the salbutamol standard liquid in step a with testing sample, according in step b and c
Method detected, according to response current reduction difference DELTAIAnd working curve, obtain containing for salbutamol in testing sample
Amount.
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| CN201610101697.0A CN105699645B (en) | 2016-02-25 | 2016-02-25 | A kind of preparation method and application of electrochemistry salbutamol sensor |
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| CN107356640B (en) * | 2017-07-10 | 2019-08-27 | 山东利源康赛环境咨询有限责任公司 | A kind of optical electro-chemistry ethinyloestradiol immunosensor and its preparation and application based on two-dimentional multi-element metal composite nano materials |
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