CN106119319A - 重组α‑L‑鼠李糖苷酶粗提液用于微通道反应器中催化定向水解黄酮苷的方法 - Google Patents
重组α‑L‑鼠李糖苷酶粗提液用于微通道反应器中催化定向水解黄酮苷的方法 Download PDFInfo
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- CN106119319A CN106119319A CN201610723949.3A CN201610723949A CN106119319A CN 106119319 A CN106119319 A CN 106119319A CN 201610723949 A CN201610723949 A CN 201610723949A CN 106119319 A CN106119319 A CN 106119319A
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- rhamnosidase
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- flavonoid glycoside
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/0104—Alpha-L-rhamnosidase (3.2.1.40)
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CN201610723949.3A CN106119319B (zh) | 2016-08-25 | 2016-08-25 | 重组α-L-鼠李糖苷酶粗提液用于微通道反应器中催化定向水解黄酮苷的方法 |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107287223A (zh) * | 2017-06-20 | 2017-10-24 | 江苏科技大学 | α‑L‑鼠李糖苷酶基因及其应用 |
CN108949658A (zh) * | 2018-06-15 | 2018-12-07 | 江苏科技大学 | 一种增大通透性的全细胞催化剂及其制备方法和应用 |
CN110272929A (zh) * | 2019-06-10 | 2019-09-24 | 江苏科技大学 | 一种利用微液滴催化芦丁产异槲皮苷的酶催化反应体系 |
CN111500661A (zh) * | 2020-04-23 | 2020-08-07 | 江南大学 | 一种同时制备l-鼠李糖和异槲皮素的方法 |
WO2021232840A1 (zh) * | 2020-05-20 | 2021-11-25 | 江苏科技大学 | 一种α-L-鼠李糖苷酶的截短突变体及其应用 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102286576A (zh) * | 2011-09-14 | 2011-12-21 | 江苏科技大学 | 强化酶法合成异槲皮苷的介质工程方法 |
CN102876746A (zh) * | 2012-10-11 | 2013-01-16 | 江苏科技大学 | 离子液体共溶剂效应强化酶促合成异槲皮苷的方法 |
CN103627756A (zh) * | 2013-11-26 | 2014-03-12 | 江苏科技大学 | 利用连续流动的管式微反应器酶促合成异槲皮苷的方法 |
CN104312996A (zh) * | 2014-09-26 | 2015-01-28 | 南京林业大学 | α-L-鼠李糖苷酶Rha1及其表达基因和应用 |
CN105176843A (zh) * | 2015-10-22 | 2015-12-23 | 江南大学 | 一株产α-L-鼠李糖苷酶的菌株及采用该菌株发酵生产α-L-鼠李糖苷酶的方法 |
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2016
- 2016-08-25 CN CN201610723949.3A patent/CN106119319B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102286576A (zh) * | 2011-09-14 | 2011-12-21 | 江苏科技大学 | 强化酶法合成异槲皮苷的介质工程方法 |
CN102876746A (zh) * | 2012-10-11 | 2013-01-16 | 江苏科技大学 | 离子液体共溶剂效应强化酶促合成异槲皮苷的方法 |
CN103627756A (zh) * | 2013-11-26 | 2014-03-12 | 江苏科技大学 | 利用连续流动的管式微反应器酶促合成异槲皮苷的方法 |
CN104312996A (zh) * | 2014-09-26 | 2015-01-28 | 南京林业大学 | α-L-鼠李糖苷酶Rha1及其表达基因和应用 |
CN105176843A (zh) * | 2015-10-22 | 2015-12-23 | 江南大学 | 一株产α-L-鼠李糖苷酶的菌株及采用该菌株发酵生产α-L-鼠李糖苷酶的方法 |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107287223A (zh) * | 2017-06-20 | 2017-10-24 | 江苏科技大学 | α‑L‑鼠李糖苷酶基因及其应用 |
CN108949658A (zh) * | 2018-06-15 | 2018-12-07 | 江苏科技大学 | 一种增大通透性的全细胞催化剂及其制备方法和应用 |
CN110272929A (zh) * | 2019-06-10 | 2019-09-24 | 江苏科技大学 | 一种利用微液滴催化芦丁产异槲皮苷的酶催化反应体系 |
CN110272929B (zh) * | 2019-06-10 | 2023-11-21 | 江苏科技大学 | 一种利用微液滴催化芦丁产异槲皮苷的酶催化反应体系 |
CN111500661A (zh) * | 2020-04-23 | 2020-08-07 | 江南大学 | 一种同时制备l-鼠李糖和异槲皮素的方法 |
WO2021232840A1 (zh) * | 2020-05-20 | 2021-11-25 | 江苏科技大学 | 一种α-L-鼠李糖苷酶的截短突变体及其应用 |
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CN106119319B (zh) | 2019-09-27 |
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