CN106105816A - A kind of method using CNT regulation and control lawn composting substrate enzymatic activity - Google Patents

A kind of method using CNT regulation and control lawn composting substrate enzymatic activity Download PDF

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CN106105816A
CN106105816A CN201610443729.5A CN201610443729A CN106105816A CN 106105816 A CN106105816 A CN 106105816A CN 201610443729 A CN201610443729 A CN 201610443729A CN 106105816 A CN106105816 A CN 106105816A
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soil
cnt
lawn
enzymatic activity
graphene
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赵树兰
多立安
白雪
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Tianjin University
Tianjin Normal University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F9/00—Fertilisers from household or town refuse
    • C05F9/04—Biological compost
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

The invention discloses a kind of method using CNT regulation and control lawn composting substrate enzymatic activity, it is to use plastic tub dress soil 1500 g, and every basin adds 50 g consumer garbage composts, combines turf establishment substrate with soil；Carbon nanomaterial Graphene, graphene oxide and CNT are all in the 1%(w/w of soil quality) ratio interpolation, with the process without carbon nanomaterial for comparison, every basin is sown into lawn plant tall fescue seed 5 g, test and carry out in indoor, whole test lasts 130 d, cut first batch of grass in the 65th d, measure the various enzymatic activity of compounded medium.Experimental result shows: add after CNT the active increase rate of lawn matrix protease 17.2%.The method is that carbon nanomaterial is applied to Turf ecology planting and provides technical support.

Description

A kind of method using CNT regulation and control lawn composting substrate enzymatic activity
Technical field
The invention belongs to environmental protection technical field, relate to a kind of employing CNT regulation and control lawn composting substrate enzymatic activity Method.
Background technology
In 21 century, nano material is one of material of great development prospect in having become, it is estimated that, there is now on the market The product containing nano material more than 800 kinds, is widely used in every field.Wherein, to be that a class has important for carbon nanomaterial The nano material of using value, only during 2009 to 2010 years, the carbon nanomaterial in the whole world rises to 4065 from 2190 t t.At present, there are Graphene, graphene oxide and CNT etc. by relatively carbon nanomaterials paid close attention to more.Graphene is near A kind of carbon nanomaterial emerging over Nian, is the cellular two dimensional surface knot formed by the C atom hexagonal array of sp2 hydridization Structure material, within 2010, Nobel Prize in physics has just awarded to carry out Graphene the scientist of remarkable research.Graphene oxide is The derivant of Graphene, there is multiple group on its surface.The oxy radical that graphenic surface has is conducive to its hydrophilic, and oxygen-containing Group can be had an effect with heavy metal.CNT refers to the seamless hollow tube that graphene sheet layer is rolled into, and its pipe shaft is Being made up of carbon hexagon, sp2 hydridization is C atom main hydridization form, also can be mixed with sp3 hydridization simultaneously.The property of carbon nanomaterial Matter is tight with its structural relation, is mainly manifested in electrical and mechanical character and good absorption property etc., now extensively should It is used in material, biomedicine, the energy and environment etc. multi-field.As adsorbing material, it is pollutant in environment remediation field Absorption is notable with effect in passivation.
Nowadays, the mankind be faced with cause the energy consume excessively, the problems such as environmental pollution and global warming, its A large amount of uses of middle heavy metallic mineral, make facing mankind acid test.Heavy metal ion toxicity is greatly, disperse for a long time Exist, due to enrichment in food chain, its harm be exaggerated, and and be difficult to by biological metabolism, and then threaten human health. How to solve heavy metal problem the most noticeable.Carbon nanomaterial, because having the character such as superhigh specific surface area, is being inhaled There are high reaction efficiency and action effect obvious when of attached effect, are constantly subjected to pay attention to.Have under research different condition and aoxidize Graphene is on Pb(II in water body) impact of accumulation rate, find when temperature at 60 DEG C when, accumulation rate reaches 1850 mg/g；Have People once used the single-layer carbon nano-tube after being processed by sodium hypochlorite and the Zn in multilayer carbon nanotube enrichment water body2+, find at pH= 7, adsorption capacity is 43.66 and 32.68 mg/g respectively under the conditions of t=25 DEG C；Modify graphite oxide by introducing-SH, improve Its hydrophilic and absorption property；And it is prepared for GNSPF6Material, finds that it is to Pb2+And Cd2+Adsorption effect is good, respectively up to 406.6 mg/g and 73.42 mg/g.
The general name of the enzyme classes that soil enzyme is present in soil, have now been found that there are about 60 kinds, derives from plant roots The secretion release of system, animal and microorganism.The most sustainable generation of various Biochemical processes in soil, just has benefited from soil Playing an active part in of enzyme, owing to its activity is closely related with soil physico-chemical property etc., so always can be as evaluating soil quality Important indicator.Soil enzyme, is the biocatalyzer of soil, has important function, take part in soil in terrestrial ecosystems Whole Biochemical processes, such as organic decomposition and the circulation etc. of nutrient.Soil enzyme activities represents soil metabolism Situation, it is also possible to reflect the situation of change of nutrient situation, is to evaluate soil fertility and the important indicator of soil quality.Additionally, Soil enzyme activities is disturbed degree response sensitivity to soil, can reflect the soil quality change under different situations, and then evaluate soil Earth control measures whether reasonable benefit/risk.
The concern of soil enzyme was primarily upon in terms of fertility by researcher in the past, later constantly going out because of environmental problem Existing, soil enzyme is being attracted attention about receiving in some problems of heavy metal.Current research includes the improvement of crop and pollutant Deng, the soil enzyme related generally to has protease, catalase, urase, saccharase and phosphatase etc..Research worker is tried with Semen Maydis Testing material, the research carbon nanomaterial facilitation mechanism to plant growth, result shows the nano-carbon material work to soil enzyme Property and Root Activity in Maize have facilitation.
Domestic waste refers to the various solid waste that the resident in city is typically dropped in the middle of daily life General name, it mainly includes ground ash, TRADE REFUSE and fairground rubbish, rubbish from cooking etc..In China, along with city develops rapidly, city The generation in city also increases, and it is more complicated that component of refuse more complicated also result in environmental problem, has become as now Carry out one of focus of society common concern.Rubbish is garbage and available special resource, therefore the money of rubbish Source utilization receives to be paid attention to widely.If rubbish is rationally disposed, the energy can be saved, reduce and pollute and be conducive to economy to send out Exhibition, if mishandling, then can cause crisis to ecological environment.At home and abroad, component of refuse, natural conditions, energy resource structure, warp The difference of Ji level of development and traditional habit causes the processing method that rubbish is not uniformly processed.At present, China gives birth to The mode of garbage disposal alive mainly includes burning, landfill and compost etc..Wherein burning disposal proportion is about 2%, required operation Cost is the hugest, and can produce the pollutant such as dust, toxic and harmful, heavy metal in burning process, further results in two Secondary pollution.Sanitary landfills scarcely conformance with standard, and a large amount of land resource can be occupied, in the soil short period being utilized not Original purposes can be returned to, cause land resource serious waste.Composting rubbish is to be widely used at present and can provide Source utilizes the approach of compost.The rubbish of composted can realize rubbish from naturally again returning to nature, can reach process useless Multiple purposes such as thing and improvement soil, additionally, its end product is organic fertilizer, can send out in agricultural, grass cultivation and production of forestry Wave potentiality.
Lawn system is the ecological engineering gathered multi-function in integral whole, and has the effect viewed and admired Yu beautify, and can give pleasure to for people Pleasure, leisure and the motion of appropriateness, be the important component part of urban Compound greening.Turf establishment system can improve resident living matter Amount, as purified air, reducing noise etc., the effect such as the development of the city tourism that also functions to attract foreign investment, promote, to urban life Various aspects all there is important function, be more and more subject to people's attention.In general, if to carry out the life of a turf Produce and at least need 2 cm soil, so long-term production then can welding.In recent years, quite a few urban lawn is to use old city Removal ground, landfill produce, and in general soil property is poor, therefore soil needed for lawn add fertilizer to improving lawn Quality, ensure that lawn has good state and have positive role term.And using compost as fertilizer application in greening system both Heavy metal in compost can be made to avoid food chain, realize the recycling of resource, also solve the problem that the nutrient supply problem of lawn soil. There are some researches show, compost can promote the Biomass of lawn plant, density and degeneration-resistant border ability, and to leaf color and uniformity Also there is actively impact.In view of above-mentioned analysis, if composting substrate heavy metal passivation in lawn is fixed in turf establishment system, then Compost turf establishment system application mode can be made more safe and effective.
In China, increasing due to population and city develops rapidly, house refuse increases increasingly, rubbish the environment caused Problem is noticeable.Rubbish carries out the important channel that composted is recycling house refuse, and its product is advantageous to Improvement soil environment.And compost is applied the quality that can either improve lawn soil at turf establishment system, beneficially lawn is planted Thing ensures the best growth, additionally it is possible to recycling consumer garbage compost.
Summary of the invention
A kind of method using CNT regulation and control lawn composting substrate enzymatic activity, it is characterised in that enter by the steps OK:
(1) material
The main physical and chemical of consumer garbage compost is: pH 7.49, electrical conductivity 2300 S/cm, organic 131.84 g/kg, Full phosphorus 6.81 g/kg, full nitrogen 25.09 g/kg, Ca content 23.33 mg/kg, Fe 30.49 g/kg, Cu 341.34 mg/kg, Zn 677.33 mg/kg, Pb 216.98 mg/kg, Cd 5.02 mg/kg, Mn 437.88 mg/kg, Cr 702.60 mg/ Kg, Ni 41.82 mg/kg.
Being Tianjin Normal University's campus soil for examination soil, its main physical and chemical is as follows: water content 19.4%, pH 7.27, electrical conductivity 2250 S/cm, organic 52.29 g/kg, full phosphorus 3.75 g/kg, full nitrogen 2.15 g/kg.
Consumer garbage compost, from Tianjin little Dian destructor plant, is pulverized after being classified by consumer garbage compost.
Lawn plant selection Festuca Arundinacea (Festuca arundinacea.).
Graphene (Graphene) microplate is produced by Nanjing Ji Cang nanosecond science and technology company limited, for the random thin slice of black Shape, flit size 0.5-20 m, specific surface area 40-60 m2/g；
Graphene oxide (Graphene Oxide) is purchased from Suzhou Heng Qiu nanometer company, for black or brownish yellow powder, averagely thickness Degree 3.4-7 nm, lamella diameter 10-50 m, specific surface area 100-300 m2/g；CNT (Carbon nanotube) produces Space high-tech new material technology company limited, diameter 20-40 nm, length 10-30 m, specific surface area ＞ 110 is won from Beijing m2/g。
(2) test method:
Test uses plastic tub, and high 15 cm, internal diameter 20 cm fill soil, and every basin dress campus soil 1500 g(is according to soil moisture, folding Synthesis dry ground soil weighs 1500 g), and every basin adds 50 g consumer garbage composts, combines turf establishment substrate with soil；Carbon nanometer Material Graphene (G), graphene oxide (GO) and CNT (CNT) are all in the 1%(w/w of soil quality) ratio interpolation, with not The process adding carbon nanomaterial is comparison, and soil, consumer garbage compost fill basin after mixing with carbon nanomaterial, and every basin is sown into grass Level ground plant tall fescue seed 5 g, tests and carries out in indoor, plastic tub randomly discharging in every 5 d shift positions, temperature during cultivation Degree is for 15-26 DEG C, and relative humidity 35-55%, illumination condition is the incident illumination under natural conditions, and on-demand scales/electronic balance weighing is mended Water, whole test lasts 130 d, cuts first batch of grass in the 65th d, first rejects foreign material in fresh matrix sample, crosses 1mm sieve, mixing After put into sample sack and store in 4 DEG C, measure the various enzymatic activity of compounded medium.
The present invention further discloses and use the method for CNT regulation and control lawn composting substrate proteinase activity improving Application in terms of lawn matrix proteinase activity.Experimental result shows: the activity of lawn matrix protease after interpolation CNT Increase rate 17.2%.
More detailed description of the present invention is as follows:
1 develops materials and methods
1.1 material
The main physical and chemical of consumer garbage compost is: pH 7.49, electrical conductivity 2300 S/cm, organic 131.84 g/kg, Full phosphorus 6.81 g/kg, full nitrogen 25.09 g/kg, Ca content 23.33 mg/kg, Fe 30.49 g/kg, Cu 341.34 mg/kg, Zn 677.33 mg/kg, Pb 216.98 mg/kg, Cd 5.02 mg/kg, Mn 437.88 mg/kg, Cr 702.60 mg/ Kg, Ni 41.82 mg/kg.
Being Tianjin Normal University's campus soil for examination soil, its main physical and chemical is as follows: water content 19.4%, pH 7.27, electrical conductivity 2250 S/cm, organic 52.29 g/kg, full phosphorus 3.75 g/kg, full nitrogen 2.15 g/kg.
Consumer garbage compost, from Tianjin little Dian destructor plant, is pulverized after being classified by consumer garbage compost.Lawn Plants selection Festuca Arundinacea (Festuca arundinacea.).
Graphene (Graphene) microplate is produced by Nanjing Ji Cang nanosecond science and technology company limited, for the random thin slice of black Shape, flit size 0.5-20 m, specific surface area 40-60 m2/g。
Graphene oxide (Graphene Oxide) is purchased from Suzhou Heng Qiu nanometer company, for black or brownish yellow powder, puts down All thickness 3.4-7 nm, lamella diameter 10-50 m, specific surface area 100-300 m2/g。
CNT (Carbon nanotube) originates from Beijing wins space high-tech new material technology company limited, diameter 20- 40 nm, length 10-30 m, specific surface area ＞ 110 m2/g。
1.2 Technology design
Test use plastic tub (high 15 cm, internal diameter 20 cm) dress soil, every basin dress campus soil 1500 g(according to soil moisture, It is converted into dry ground soil and weighs 1500 g).Every basin adds 50 g consumer garbage composts, combines turf establishment substrate with soil.Carbon is received Rice material Graphene (G), graphene oxide (GO) and CNT (CNT) are all added in 1% ratio of soil quality.Not add The process adding carbon nanomaterial is comparison, and each process arranges 3 repetitions.Soil, consumer garbage compost mix with carbon nanomaterial Rear dress basin, every basin is sown into lawn plant tall fescue seed 5 g.Testing and carry out in indoor, plastic tub randomly discharging also moves in every 5 d Dynamic position, during cultivation, temperature is 15-26 DEG C, relative humidity 35-55%, and illumination condition is the incident illumination under natural conditions, on-demand Use scales/electronic balance weighing moisturizing, whole test to last 130 d, cut first batch of grass in the 65th d.
1.3 method of production
After test lasts 130 d, cradle, first reject foreign material in fresh matrix sample, cross 1mm sieve, after mixing, put into sample sack And store in 4 DEG C, measure the various enzymatic activity of compounded medium.
Using ninhydrin colorimetry to carry out the mensuration of stromatin enzymatic activity, result cultivates 24 h with 30 DEG C, in 1 g substrate The quality (mg) of amino nitrogen represents.
1.4 data statisticss and process
Use SPSS 17.0 software to complete data to process, utilize one-way ANOVA one factor analysis of variance to carry out difference and show Work is analyzed, and uses SigmaPlot 12.5 to carry out the making of chart.
2 development results analyses
Substrate enzyme activity (mg/g) under table 1 different disposal
As shown in Table 1, four kinds process lower proteinase activity size as CNT > comparison > graphene oxide > Graphene, The proteinase activity of comparison, Graphene process group, graphene oxide process group and CNT process group is respectively 3.89, 2.94,3.13 and 4.56 mg/g.Compared with the control, although adding Graphene and graphene oxide in carbon nanomaterial process group Proteinase activity under Chu Liing decreases 24.4% and 19.5% respectively, and CNT process group adds 17.2%, but at each Between reason difference notable (P> 0.05).
3 develop conclusion
Protease can promote to hydrolyze the protein in soil and peptide etc., and its product is one of N source of plant.It addition, protease and N The index such as mineralising and ATP is closely related, and relevant with the content of organic matter in soil and other soil property.Be not added with carbon The comparison of nano material is compared, and after adding CNT, the activity of lawn matrix protease substantially increases, and increase rate is 17.2%。
Detailed description of the invention:
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is Method known in those skilled in the art.It addition, embodiment is interpreted as illustrative, and the model of the unrestricted present invention Enclosing, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this On the premise of bright spirit and scope, the various changes or the change that carry out the material component in these embodiments and consumption also belong to In protection scope of the present invention.The present invention is raw materials used, reagent is commercially available.
Embodiment 1
(1) material
The main physical and chemical of consumer garbage compost is: pH 7.49, electrical conductivity 2300 S/cm, organic 131.84 g/kg, Full phosphorus 6.81 g/kg, full nitrogen 25.09 g/kg, Ca content 23.33 mg/kg, Fe 30.49 g/kg, Cu 341.34 mg/kg, Zn 677.33 mg/kg, Pb 216.98 mg/kg, Cd 5.02 mg/kg, Mn 437.88 mg/kg, Cr 702.60 mg/ Kg, Ni 41.82 mg/kg.
Being Tianjin Normal University's campus soil for examination soil, its main physical and chemical is as follows: water content 19.4%, pH 7.27, electrical conductivity 2250 S/cm, organic 52.29 g/kg, full phosphorus 3.75 g/kg, full nitrogen 2.15 g/kg.
Consumer garbage compost, from Tianjin little Dian destructor plant, is pulverized after being classified by consumer garbage compost.
Lawn plant selection Festuca Arundinacea (Festuca arundinacea.).
Graphene (Graphene) microplate is produced by Nanjing Ji Cang nanosecond science and technology company limited, for the random thin slice of black Shape, flit size 10 m, specific surface area 40 m2/g；
Graphene oxide (Graphene Oxide) is purchased from Suzhou Heng Qiu nanometer company, for black or brownish yellow powder, averagely thickness Spend 3.4 nm, lamella diameter 10 m, specific surface area 100 m2/g；CNT (Carbon nanotube) originates from Beijing wins space High-tech new material technology company limited, diameter 20 nm, length 10 m, specific surface area ＞ 110 m2/g。
(2) test method:
Test uses plastic tub, and high 15 cm, internal diameter 20 cm fill soil, and every basin dress campus soil 1500 g(is according to soil moisture, folding Synthesis dry ground soil weighs 1500 g), and every basin adds 50 g consumer garbage composts, combines turf establishment substrate with soil；Carbon nanometer Material Graphene (G), graphene oxide (GO) and CNT (CNT) are all in the 1%(w/w of soil quality) ratio interpolation, with not The process adding carbon nanomaterial is comparison, and soil, consumer garbage compost fill basin after mixing with carbon nanomaterial, and every basin is sown into grass Level ground plant tall fescue seed 5 g, tests and carries out in indoor, plastic tub randomly discharging in every 5 d shift positions, temperature during cultivation Degree is 15 DEG C, relative humidity 35%, and illumination condition is the incident illumination under natural conditions, and on-demand scales/electronic balance weighing moisturizing is whole Test lasts 130 d, cuts first batch of grass in the 65th d, first rejects foreign material in fresh matrix sample, crosses 1mm sieve, put into after mixing Store to sample sack and in 4 DEG C, measure the various enzymatic activity of compounded medium.
Embodiment 2
(1) material
The main physical and chemical of consumer garbage compost is: pH 7.49, electrical conductivity 2300 S/cm, organic 131.84 g/kg, Full phosphorus 6.81 g/kg, full nitrogen 25.09 g/kg, Ca content 23.33 mg/kg, Fe 30.49 g/kg, Cu 341.34 mg/kg, Zn 677.33 mg/kg, Pb 216.98 mg/kg, Cd 5.02 mg/kg, Mn 437.88 mg/kg, Cr 702.60 mg/ Kg, Ni 41.82 mg/kg.
Being Tianjin Normal University's campus soil for examination soil, its main physical and chemical is as follows: water content 19.4%, pH 7.27, electrical conductivity 2250 S/cm, organic 52.29 g/kg, full phosphorus 3.75 g/kg, full nitrogen 2.15 g/kg.
Consumer garbage compost, from Tianjin little Dian destructor plant, is pulverized after being classified by consumer garbage compost.
Lawn plant selection Festuca Arundinacea (Festuca arundinacea.).
Graphene (Graphene) microplate is produced by Nanjing Ji Cang nanosecond science and technology company limited, for the random thin slice of black Shape, flit size 20 m, specific surface area 60 m2/g；
Graphene oxide (Graphene Oxide) is purchased from Suzhou Heng Qiu nanometer company, for black or brownish yellow powder, averagely thickness Spend 7 nm, lamella diameter 50 m, specific surface area 300 m2/g；CNT (Carbon nanotube) originates from Beijing wins space height Science and technology new material technology company limited, diameter 40 nm, length 30 m, specific surface area ＞ 110 m2/g。
(2) test method:
Test uses plastic tub, and high 15 cm, internal diameter 20 cm fill soil, and every basin dress campus soil 1500 g(is according to soil moisture, folding Synthesis dry ground soil weighs 1500 g), and every basin adds 50 g consumer garbage composts, combines turf establishment substrate with soil；Carbon nanometer Material Graphene (G), graphene oxide (GO) and CNT (CNT) are all in the 1%(w/w of soil quality) ratio interpolation, with not The process adding carbon nanomaterial is comparison, and soil, consumer garbage compost fill basin after mixing with carbon nanomaterial, and every basin is sown into grass Level ground plant tall fescue seed 5 g, tests and carries out in indoor, plastic tub randomly discharging in every 5 d shift positions, temperature during cultivation Degree is 26 DEG C, relative humidity 50%, and illumination condition is the incident illumination under natural conditions, and on-demand scales/electronic balance weighing moisturizing is whole Test lasts 130 d, cuts first batch of grass in the 65th d, first rejects foreign material in fresh matrix sample, crosses 1mm sieve, put into after mixing Store to sample sack and in 4 DEG C, measure the various enzymatic activity of compounded medium.

Claims (4)

1. the method using CNT regulation and control lawn composting substrate enzymatic activity, it is characterised in that enter by the steps OK:
(1) material
Select consumer garbage compost from Tianjin little Dian destructor plant, pulverize after consumer garbage compost is classified；
It is Tianjin Normal University's campus soil for examination soil；
Lawn plant selection Festuca Arundinacea (Festuca arundinacea.)；
Graphene microchip size 0.5-20 m, specific surface area 40-60 m2/g；
Graphene oxide average thickness 3.4-7 nm, lamella diameter 10-50 m, specific surface area 100-300 m2/g；
Carbon nanotube diameter 20-40 nm, length 10-30 m, specific surface area ＞ 110 m2/g；
(2) test method:
Test uses plastic tub, and high 15 cm, internal diameter 20 cm fill soil, and every basin dress campus soil 1500 g, it is raw that every basin adds 50 g Garbage compost alive, combines turf establishment substrate with soil；Carbon nanomaterial Graphene (G), graphene oxide (GO) and carbon are received Mitron (CNT) is all in the 1%(w/w of soil quality) ratio interpolation, with the process without carbon nanomaterial for comparison, soil, life Living after garbage compost mixes with carbon nanomaterial and fill basin, every basin is sown into lawn plant tall fescue seed 5 g, tests and enter in indoor OK, plastic tub randomly discharging in every 5 d shift positions, during cultivation, temperature is 15-26 DEG C, relative humidity 35-55%, illumination Condition is the incident illumination under natural conditions, on-demand scales/electronic balance weighing moisturizing, and whole test lasts 130 d, cuts in the 65th d First batch of grass, first rejects foreign material in fresh matrix sample, crosses 1mm sieve, puts into sample sack and store in 4 DEG C after mixing, Measure the various enzymatic activity of compounded medium.
2. the method described in claim 1, wherein the main physical and chemical of consumer garbage compost is: pH 7.49, electrical conductivity 2300 S/cm, organic 131.84 g/kg, full phosphorus 6.81 g/kg, full nitrogen 25.09 g/kg, Ca content 23.33 mg/kg, Fe 30.49 g/kg, Cu 341.34 mg/kg, Zn 677.33 mg/kg, Pb 216.98 mg/kg, Cd 5.02 mg/kg, Mn 437.88 mg/kg, Cr 702.60 mg/kg, Ni 41.82 mg/kg；
Being Tianjin Normal University's campus soil for examination soil, its main physical and chemical is as follows: water content 19.4%, pH 7.27, electricity Conductance 2250 S/cm, organic 52.29 g/kg, full phosphorus 3.75 g/kg, full nitrogen 2.15 g/kg；
Lawn plant selection Festuca Arundinacea (Festuca arundinacea.)；
Graphene microchip size 0.5-20 m, specific surface area 40-60 m2/g；
Graphene oxide average thickness 3.4-7 nm, lamella diameter 10-50 m, specific surface area 100-300 m2/g；
Carbon nanotube diameter 20-40 nm, length 10-30 m, specific surface area ＞ 110 m2/g。
3. the method using CNT regulation and control lawn composting substrate enzymatic activity described in claim 1 is improving lawn matrix egg Application in terms of white enzymatic activity.
4. the application described in claim 3, the wherein active increase rate of lawn matrix protease after interpolation CNT 17.2%。
CN201610443729.5A 2016-06-21 2016-06-21 A kind of method using CNT regulation and control lawn composting substrate enzymatic activity Pending CN106105816A (en)

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