CN106086180A - A kind of molecule labelling method of lodging resistance in rice main effect QTL qSR5.1 - Google Patents
A kind of molecule labelling method of lodging resistance in rice main effect QTL qSR5.1 Download PDFInfo
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Abstract
The present invention relates to crop molecular breeding technology field, a kind of molecule labelling method of lodging resistance in rice main effect QTL qSR5.1, utilize with the primer of main effect QTL qSR5.1 resistant to lodging closely linked molecular marker RM5796 to and the primer pair of ILP5 11, the DNA of breeding population is carried out PCR amplification, as amplified the DNA fragmentation of corresponding size, then indicate the existence of main effect QTL qSR5.1 resistant to lodging.The present invention uses the molecule labelling method of lodging resistance in rice main effect QTL qSR5.1, the method be application screen with closely linked 2 molecular markers of main effect QTL qSR5.1 resistant to lodging that navigate on No. 5 chromosome of lodging resistance in rice kind 023S, they can effectively predict the lodging resistance of rice breeding colony, accelerates the selection-breeding speed of kind resistant to lodging.
Description
Technical field
The present invention relates to crop molecular breeding technology field, specifically a kind of lodging resistance in rice main effect QTL qSR5.1
Molecule labelling method.
Background technology
China is the first big country of world's Rice Production and consumption, and within 2011, domestic Monitoring of Paddy Rice Plant Area about 33,000,000 is public
Hectare, annual production about 1.0-2.6 hundred million tons, account for the 35% of world's Oryza glutinosa total output.Owing to Chinese people mouth pressure is big, available cultivated area
Day by day reducing, the pressure improving China's rice yield is the hugest, and rice lodging, it always is the weight that Rice Production is faced
Want problem, and be that restriction rice mechanization produces one of of paramount importance factor.
Lodging generally exists in crop production.The generation of lodging, has not only upset the distribution space order of each organ of plant
Sequence, makes the photosynthetic efficiency of blade reduce, and under many circumstances, because stem stalk fractures, causes conducting system to cause destruction, and then impact
The transport of moisture, mineral nutrition and assimilation quotient, even results in photosynthesis and the stopping of cereal crops seed irrigation.According to report
Road, plant lodging causes the production loss of the staple food crops such as Semen Tritici aestivi, Semen Maydis and Oryza sativa L. up to 20-50%, and makes quality
Serious deterioration.For a long time, lodging problem is always one of numerous content of breeding scholar's primary study.The anti-fall energy of Oryza sativa L.
Power and plant type especially Stalk characters is closely related, is also affected by cultivation condition and external environment simultaneously.The fifties in last century,
The popularization of short-stalked variety brings resistance to fertilizer, the advantage such as be substantially improved of resistant to lodging and yield, but is as the big of short-stalked variety
Area popularization and application, increasing researcher finds that yield needs to improve biological yield and can be only achieved product after reaching certain level
The increase of amount, and the effective way effectively broken through realizing biological yield is just to increase plant height.Increasing however as plant height
Add will again let us in the face of lodging risk.So, the approach resistant to lodging beyond reduction plant height of exploring just becomes water
The important research content of the further high yield of rice.
Weigh at present lodging resistance in rice could the index of power a lot, be concentrated mainly on the relation of lodging characteristics and morphological characters
Etc. aspect, lodging property has close ties with the character such as plant height, strength of stem, Root morphology.There is a small amount of genetic research in recent years
Report, be concentrated mainly on the hereditary variation between stem stalk internode character and relevant analysis；Or from genecology angle analysis
The genotype by environment interaction aspect that affects on lodging resistance in rice, and the cytologic mechanism of rice lodging and molecular genetics
Aspect research is the most relatively fewer.Therefore, the excavation accelerating lodging resistance in rice related gene is to lose lodging resistance in rice molecule
Pass and learn further investigation and the effective measures of the strong improved seeds of selection-breeding lodging resistance, be the most also to improve having of rice quality and yield
Effect approach.Although having navigated to some relevant QTLs resistant to lodging to Culm of Rice at present, but these QTL being to lodging resistance in rice
Contribute less, and these labelling repeatability are poor, utilize extremely difficult on rice breeding.
In Oryza sativa L. conventional breeding methods, character resistant to lodging could be identified to the period of maturation, and easily by environment, especially wind
The impact of rain, identifies that field error is big, and efficiency of selection is low.Detection gene resistant to lodging and relevant QTL is used to predict that Oryza sativa L. is anti-fall
Volt character, can carry out screening in seedling stage and eliminate, not only save production cost but also can be effectively improved efficiency of selection, thus accelerate
Rice breeding process.
Summary of the invention
The present invention provides the molecule labelling method of a kind of lodging resistance in rice main effect QTL qSR5.1, and the method is by breeding
Colony DNA carries out PCR amplification, can effectively detect whether breeding population has lodging resistance, can be greatly improved lodging resistance in rice and educate
The efficiency of selection planted.
For achieving the above object, the technical solution used in the present invention is: a kind of lodging resistance in rice main effect QTL qSR5.1
Molecule labelling method, utilize with the primer of main effect QTL qSR5.1 resistant to lodging closely linked molecular marker RM5796 to and
The primer pair of ILP5-11, carries out PCR amplification to the DNA of breeding population, as amplified the DNA fragmentation of corresponding size, then indicates
The existence of main effect QTL qSR5.1 resistant to lodging.
Further, the forward primer of described molecular marker RM5796 primer pair is: 5 ＇-GCGATGGAACATGAAGTGT
G-3 ＇, the reverse primer of described molecular marker RM5796 primer pair: 5 ＇-TGGATGTTCTGATGCAGAGC-3 ＇).
Further, the forward primer of described molecular marker ILP5-11 primer pair is: 5 ＇-ATCCCTGGCACGTGTCTTA
C-3 ＇, the reverse primer of described molecular marker ILP5-11 primer pair is: 5 ＇-CCCAAGCATGCGTAGTTAGAG-3 ＇.
Further, PCR reaction system is
Reaction system ddH2O supplies cumulative volume 10 μ L.
Further, PCR amplification program: 95 DEG C of denaturations 5min；95 DEG C of degeneration 30s, 55-58 DEG C of annealing 30s, 72 DEG C are prolonged
Stretch 1min, 35 circulations；Last 72 DEG C re-extend 7min, 4 DEG C of preservations.
It is a further object of the present invention to provide the molecule labelling method of a kind of lodging resistance in rice main effect QTL qSR5.1 in choosing
Educate in lodging resistance in rice kind and apply.
The method have the benefit that: the present invention uses the molecular marker side of lodging resistance in rice main effect QTL qSR5.1
Method, the method be application screen with the main effect QTL resistant to lodging that navigates on No. 5 chromosome of lodging resistance in rice kind 023S
Closely linked 2 molecular markers of qSR5.1, they can effectively be predicted the lodging resistance of rice breeding colony, accelerate product resistant to lodging
The selection-breeding speed planted.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, also may be used
To obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is that present invention main effect QTL resistant to lodging qSR5.1 is in the location of No. 5 chromosome；
Fig. 2 be the primer inventing described molecular marker RM5796 to and the primer of ILP5-11 to electrophoretogram, wherein A:
143S；B:023S；1 ... 46:BC1F2 strain.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Describe, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments wholely.Based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under not making creative work premise
Embodiment, broadly falls into the scope of protection of the invention.
Lodging resistance in rice assay method and other routine tests operation sees: the relation of Culm of Rice character and lodging resistance and
Analysis of Combining Ability, Chen Guihua etc., 2016 (3), Scientia Agricultura Sinica.
Embodiment 1
Material to be tested
The material to be tested of the present embodiment is: the Rice Germplasm Resources provided with Agricultural University Of Hunan's rice science institute
023S and 143S and 190 strain F of both hybridization2Colony is examination material, tests and sowed June 26, and July 16 transplanted, Dan Ben
Transplanting, rice transplantation by hand, rice transplanting specification line-spacing is 30cm, and spacing in the rows is 16.5cm.
Phenotypic evaluation
Test and weeded proving ground, garden in 2014 in Agricultural University Of Hunan and carry out.In Oryza sativa L. full ripe stage, Oryza sativa L. top is cut
Fall, remaining away from ground 40cm height, in rice spacing in the rows ground 20cm eminence, use plant lodging tester (YD-1, Zhejiang Top instrument
Company) it is perpendicular to rice strain stem stalk and pushes forward, rice stem is bent to 45 DEG C of tilting position (measuring with protractor), now, plant falls
Volt tester automatically records the force value of maximum, i.e. Oryza sativa L. individual plant and bears maximum racking force resistance.Single stem fracture resistence force is every strain individual plant
Fracture resistence force is divided by every strain tiller number income value.
The structure of genetic linkage maps
Utilize 1518 pairs of SSR, SFP and InDel labellings of uniform fold rice genome, carry out the polymorphic inspection between parent
Surveying, result shows, has 102 pairs of primers to present polymorphism between 143S and 023S, and wherein, it is 87 right that SSR primer has, SFP primer
There have to be 3 right, and it is 12 right that InDel primer has, and every chromosome has 9 pairs of primers, and polymorphic recall rate is 6.72%, and all primers contaminate at each bar
Amplification rate on colour solid is all more than 95%, and amplification efficiency is good.
From the polymorphic primer of 102 couple filtered out, select the polymorphic primer of 100 couple of polymorphic obvious difference, build genetic map
Spectrum.The constructed F containing 190 individual plants2It is right that the genetic map of colony comprises 90 pairs of SSR marker, 2 pairs of SFP labellings and 8
InDel labelling, cover whole rice genome 1579.3cM, and 12 chromosomes of uniform fold, between each two adjacent marker
Average distance is 17.95cM, and flag sequence is basically identical with the molecular genetic linkage map delivered.
The location of QTL resistant to lodging
In order to detect F2 colony related gene resistant to lodging or Resistance QTL s, 100 in conjunction with 190 F2 colony strains polymorphic
The genotype of labelling and the phenotypic data resistant to lodging of each strain, by answering of Windows QTL Cartographer2.5 software
Closing Interval mapping (CIM), analyzing rice relevant QTL resistant to lodging, at the molecular marker of No. 5 (shown in Fig. 1) chromosome of Oryza sativa L.
Between RM5796 to ILP5-11, navigating to a relevant QTL resistant to lodging, by its named qSR5.1, contribution rate is 40.75%.
The checking of main effect QTL qSR5.1 resistant to lodging
In order to identify the main effect QTL qSR5.1 resistant to lodging navigated to, it is carried out the separation of Monogenic lines, i.e. passes through base
Because of type analysis, select the strain only containing main effect QTL qSR5.1 resistant to lodging, this strain is measured respectively great vascular bundle number, little dimension
Tube bank number, great vascular bundle length, great vascular bundle width, small bundle length, small bundle width, little wall thickness, mechanical tissue thickness, cane
The character such as wall thickness, fracture resistence force.Result shows, the strain of contained main effect QTL qSR5.1 resistant to lodging is substantially better than parent, for rear
Continuous near isogene builds and map based cloning related gene resistant to lodging is laid a good foundation.
The phenotype checking of table 1 main effect QTL resistant to lodging qSR5.1
Embodiment 2
Material to be tested
The material to be tested of the present embodiment is: the Monogenic lines containing main effect QTL qSR5.1 resistant to lodging is miscellaneous with rice varieties 143S
Hand over and obtain BC1F1, BC1F1 bagging selfing acquisition BC1F2.
For the Monogenic lines containing main effect QTL qSR5.1 resistant to lodging, 143S and the seed of 46 BC1F2 strains of examination, in advance
37 DEG C of seed soaking (two dry two wet), after showing money or valuables one carries unintentionally, it is seeded in plastic rice seedling-raising disk.Nursery soil uses the Nutrition Soil bought on the market,
Nurse young plants in hothouses.When seedling SANYE wholeheartedly time, two parents and each strain of BC1F2 take the tender leaf of 1g respectively, use CTAB method to carry
Take genomic DNA.
With above-mentioned with main effect QTL qSR5.1 resistant to lodging closely linked molecular marker RM5796 (forward primer: 5 ＇-
GCGATGGAACATGAAGTGTG-3 ＇, reverse primer: 5 ＇--TGGATGTTCTGATGCAGAGC-3 ＇) and ILP5-11 (forward draws
Thing: 5 ＇-ATCCCTGGCACGTGTCTTAC-3, reverse primer: 5 ＇--CCCAAGCATGCGTAGTTAGAG-3 ＇) expand two
Genomic DNA detection BC1F2 each strain character resistant to lodging of parent and 46 BC1F2 strains.
PCR reaction system is: PCR amplification cumulative volume is 10 μ l, wherein, DNA profiling (20ng/ μ l) 1 μ l, primer
(2pmol/ μ l) 1 μ l, 10 × Buffer 1 μ l, 10mM dNTPs (2.5mM) 0.2 μ l, rTaq (5U/ μ l) 0.1 μ l, ddH2O
6.7μl。
PCR amplification program: 95 DEG C of denaturations 5min；Following 35 circulations: 95 DEG C of degeneration 30s, 55～58 DEG C of annealing
30s, 72 DEG C extend 1min；Last 72 DEG C re-extend 7min, 4 DEG C of preservations.
Result shows: with main effect QTL qSR5.1 resistant to lodging closely linked molecular marker RM5796 (forward primer: 5 ＇-
GCGATGGAACATGAAGTGTG-3 ＇, reverse primer: 5 ＇--TGGATGTTCTGATGCAGAGC-3 ＇) and ILP5-11 (forward draws
Thing: 5 ＇-ATCCCTGGCACGTGTCTTAC-3 ＇, reverse primer: 5 ＇-CCCAAGCATGCGTAGTTAGAG-3 ＇) 4,5,12,
17,12 BC1F2 strains such as 22,28,31,34,37,38,39,43 all can detect with electrophoresis identical with rice varieties 023S
Banding pattern, i.e. contain main effect QTL qSR5.1 resistant to lodging, be lodging resistance in rice strain (as shown in Figure 2).
Embodiment 3
Two parents in above-described embodiment 2 and 46 BC1F2 strains are planted respectively and weed garden examination in Agricultural University Of Hunan
Test base, measure the lodging resistance in rice character such as stem fracture resistence force.
Result shows: utilize and main effect QTL qSR5.1 resistant to lodging closely linked molecular marker RM5796 and ILP5-11
The method detecting breeding population resistant to lodging is consistent with the result of field test method, illustrates that the present invention utilizes main effect QTL resistant to lodging
The molecule labelling method accuracy of qSR5.1 is high.
The banding pattern that A: genotype is identical with weeding 9S；The banding pattern that B: genotype is identical with 023S；The heterozygote of H:143S/023S
Banding pattern.
Those skilled in the art of the present technique are appreciated that unless otherwise defined, and all terms used herein (include technology art
Language and scientific terminology) have with the those of ordinary skill in art of the present invention be commonly understood by identical meaning.Also should
Being understood by, those terms defined in such as general dictionary should be understood that the meaning having with the context of prior art
The meaning that justice is consistent, and unless defined as here, will not explain by idealization or the most formal implication.
It should be noted last that: above example is only in order to illustrative not limiting technical scheme, although ginseng
According to above-described embodiment, the present invention is described in detail, it will be apparent to an ordinarily skilled person in the art that: still can be to this
Invention is modified or equivalent, and any modification or partial replacement without departing from the spirit and scope of the present invention, it is equal
Should contain in the middle of scope of the presently claimed invention.
Claims (6)
1. the molecule labelling method of a lodging resistance in rice main effect QTL qSR5.1, it is characterised in that utilize and main effect resistant to lodging
The primer of QTL qSR5.1 closely linked molecular marker RM5796 to and the primer pair of ILP5-11, the DNA of breeding population is entered
Performing PCR expands, and as amplified the DNA fragmentation of corresponding size, then indicates the existence of main effect QTL qSR5.1 resistant to lodging.
The molecule labelling method of lodging resistance in rice main effect QTL qSR5.1 the most according to claim 1, it is characterised in that described
The forward primer of molecular marker RM5796 primer pair is: 5 ＇-GCGATGGAACATGAAGTGTG-3 ＇, described molecular marker
The reverse primer of RM5796 primer pair: 5 ＇-TGGATGTTCTGATGCAGAGC-3 ＇).
The molecule labelling method of lodging resistance in rice main effect QTL qSR5.1 the most according to claim 1, it is characterised in that described
The forward primer of molecular marker ILP5-11 primer pair is: 5 ＇-ATCCCTGGCACGTGTCTTAC-3 ＇, described molecular marker
The reverse primer of ILP5-11 primer pair is: 5 ＇-CCCAAGCATGCGTAGTTAGAG-3 ＇.
The molecule labelling method of lodging resistance in rice main effect QTL qSR5.1 the most according to claim 1, it is characterised in that PCR
Reaction system is
Reaction system ddH2O supplies cumulative volume 10 μ L.
The molecule labelling method of lodging resistance in rice main effect QTL qSR5.1 the most according to claim 1, it is characterised in that PCR
Amplification program: 95 DEG C of denaturations 5min；95 DEG C of degeneration 30s, 55-58 DEG C of annealing 30s, 72 DEG C extend 1min, 35 circulations；Finally
72 DEG C re-extend 7min, 4 DEG C of preservations.
6. according to the molecule labelling method of lodging resistance in rice main effect QTL qSR5.1 described in any one of claim 1-5 at selection-breeding water
Rice kind resistant to lodging is applied.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107227373A (en) * | 2017-07-31 | 2017-10-03 | 长江师范学院 | A kind of SNP Functional markers of japonica rice gene resistant to lodging and application |
| CN109295248A (en) * | 2018-10-26 | 2019-02-01 | 河南省农业科学院作物设计中心 | For detecting primer, kit, detection method and the application of the molecular labeling of control corn stem intensity main effect QTL linkage |
| CN110904266A (en) * | 2019-12-26 | 2020-03-24 | 北京市农林科学院 | Identification of maize stalk lodging resistance QTL and development and application of molecular marker |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103374616A (en) * | 2012-04-25 | 2013-10-30 | 南京农业大学 | Method for separating two tightly linked rice sterile genes |
-
2016
- 2016-06-17 CN CN201610437546.2A patent/CN106086180B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103374616A (en) * | 2012-04-25 | 2013-10-30 | 南京农业大学 | Method for separating two tightly linked rice sterile genes |
Non-Patent Citations (5)
| Title |
|---|
| ZHANG L等: "Quantitative trait locus analysis and fine mapping of the qPL6 locus for panicle length in rice.", 《THEOR APPL GENET.》 * |
| 张喜娟等: "水稻基部伸长节间性状与茎秆机械强度的相关分析和 QTL 定位", 《植物生理学通讯》 * |
| 沈英嘉: "水稻全基因组多态性数据库的构建及高效分子标记的建立", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 穆平等: "水、旱条件下水稻茎秆主要抗倒伏性状的QTL分析", 《遗传学报》 * |
| 鞠晓晨: "水稻抗倒伏相关性状QTL定位分析", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107227373A (en) * | 2017-07-31 | 2017-10-03 | 长江师范学院 | A kind of SNP Functional markers of japonica rice gene resistant to lodging and application |
| CN107227373B (en) * | 2017-07-31 | 2020-07-07 | 长江师范学院 | SNP functional molecular marker of japonica rice lodging-resistant gene and application |
| CN109295248A (en) * | 2018-10-26 | 2019-02-01 | 河南省农业科学院作物设计中心 | For detecting primer, kit, detection method and the application of the molecular labeling of control corn stem intensity main effect QTL linkage |
| CN110904266A (en) * | 2019-12-26 | 2020-03-24 | 北京市农林科学院 | Identification of maize stalk lodging resistance QTL and development and application of molecular marker |
| CN110904266B (en) * | 2019-12-26 | 2022-05-31 | 北京市农林科学院 | Identification of maize stalk lodging resistance QTL and development and application of molecular marker |
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