CN106085853A - Hairy root liquid culture device - Google Patents
Hairy root liquid culture device Download PDFInfo
- Publication number
- CN106085853A CN106085853A CN201610615411.0A CN201610615411A CN106085853A CN 106085853 A CN106085853 A CN 106085853A CN 201610615411 A CN201610615411 A CN 201610615411A CN 106085853 A CN106085853 A CN 106085853A
- Authority
- CN
- China
- Prior art keywords
- tank
- tank body
- incubator
- air inlet
- mouth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immunology (AREA)
Abstract
Hairy root liquid culture device, including tank body, tank body top is the tank mouth shunk, and tank mouth is provided with seal closure, the centrally disposed sealer of seal closure；Being respectively equipped with air inlet and fluid infusion mouth on two shouldered walls of tank body, fluid infusion mouth is connected with tank interior by feed pipe；Tank base is provided centrally with a groove, fixed gas generating means in described groove, and gas generating unit is connected with air inlet by breather.The incubator of the present invention is transparent high temperature resistant material, can the growth conditions of hairy root in real-time monitored tank body；Gas generating unit is placed in tank base, arranges fixed groove, it is achieved top-blown oxygen supply, and given vent to anger by the filter membrane being arranged on seal closure, it is located at the design bottom incubator than traditional blow vent, the fluid seepage that bottom opening causes can be prevented, and prevent exogenous pollution；In tank body, each junction all uses arc, without dead angle in tank body, prevents impurities left in incubator, it is possible to reduces fluid vortex, stablizes growth of hair root.
Description
Technical field
The present invention relates to a kind of device for cultivating plant tissue, a kind of incubator cultivating growth of hair root, belong to
Vegetable culture technique reactor engineering field.
Background technology
Utilize hairy root to cultivate production medicinal plants secondary metabolite and there are great productive potentialities, and develop and be suitable for hair
The bioreactor that shape root is cultivated, is again its industrialized basis and key.In shake-flask culture level, interior foreign scholar is
Hairy root cultivating system in establishing more than 100, it is thus achieved that different reactive compounds, but these hairy root are eventually used for expanding
Cultivate or realize the very the fewest of industrialized production.This mainly due to: (1) hairy root is easily agglomerated into close sphere
Or dough, cause the internal dissolved oxygen of culture to reduce, cause internal hairy root aging to be accelerated even dead, finally affect hairy root
Increment accumulates；(2) shearing force produced mechanical agitation or air blast is sensitive, easily causes the necrosis of growth of hair root point or training
Support thing fracture, produce wound healing or growth retardation.Hairy root needs to maintain oxyty the level of limiting growth in growth
On, different dissolved oxygen amounts are notable on growth of hair root impact, must have suitable dissolved oxygen, for hairy root cell in fluid medium
Growth and metabolism provide oxygen.Traditional method usually through the biography oxygen transfer ability of equipment oxygen delivery capacity or environment of improving in certain journey
Improve the transfer rate of oxygen on degree, but all limited by equipment and energy consumption, affect its large-scale culture.
At present, the hairy root of a few species, by improving incubator performance, is achieved on a large scale by part researcher
Amplification culture and industrialized production, create good economic benefit and social benefit.The most successfully there is atomization biology
Incubator, bubble type incubator and screen cloth gas-lifting type containers for culturing organisms etc..But hairy root incubator of the prior art exists for
Oxygen stream leaves dead angle and is easily caused generation eddy current or impurities left in producing excessive shearing force, incubator, thus affects hair
Shape root produces, and even results in hairy root fracture or dead.
Summary of the invention
It is easily caused hairy root by mechanical injuries for solving the offering oxygen way of hairy root incubator of the prior art,
Being unfavorable for that hairy root is unfolded and grows, dissolved oxygen diffusion is uneven, and is easily caused culture fluid leakage, causes owing to adding nutritional solution
The problem of exogenous pollution, the present invention intends providing a kind of dissolved oxygen diffusion uniformly, the more conducively incubator of growth of hair root.
Hairy root liquid culture device provided by the present invention, including tank body, tank body top is the tank mouth shunk, and tank mouth sets
There is seal closure, the centrally disposed sealer of seal closure；It is respectively equipped with air inlet on two shouldered walls of tank body and stream adds fluid infusion mouth, tank body
Bottom centre is provided with a groove, fixed gas generating means in described groove, and gas generating unit passes through air inlet pipe and enters
QI KOU connects.
In above-mentioned incubator, as the most preferably, described air inlet pipe is hard tube, is cased with one layer of rubber outside it
Set.Air inlet pipe uses the flow perturbation that hard tube is possible to prevent air inlet to cause, and the rubber sleeve of outside then facilitates air inlet pipe and gas
Body generating means and the connection of the outside each device of tank body.
In above-mentioned incubator, as the most preferably, described sealer is one-way ventilating film, as the most real
Executing one of mode, described one-way ventilating film is the micropore sterilised membrane filter of ventilative impermeable bacterium.Gas can discharge tank body by this filter membrane,
Gas outlet is located on top cover by the present invention, can avoid nutritional solution seepage, reduces energy consumption and prevents exogenous pollution.
In above-mentioned incubator, as the most preferably, BULKHEAD UNION is installed, outside tank body at described air inlet
Filter it is also associated with in air inlet pipe.
In above-mentioned incubator, as further preferably, at the bottom of described tank mouth and tank body wall, tank body wall and tank body
Portion, the groove of tank base and tank body Shang Ge corner are arc and connect design, such smooth type connected mode without dead angle,
Impurities left in incubator can be prevented, also can reduce fluid vortex simultaneously, stablize growth of hair root；The size of described groove is with energy
Accommodating gas generating unit to be advisable, the projection of tank base groove and female all use smooth cambered design, can realize
Dissolved oxygen diffusion uniformly, reduces air whirl；It addition, gas generating unit is placed in tank base, top-blown oxygen supply can be realized.
In above-mentioned incubator, as the most preferably, the material of described tank body is transparent high temperature resistant material, is suitable for seeing
Examine hairy root plant growth conditions in incubation.
In above-mentioned incubator, as further preferably, the width of described tank mouth and the width ratio of tank body main body be 1:2～
3；The total length ratio of the projection of tank base and the aggregate level length of female and tank base is 1:2～4.
In above-mentioned incubator, as one of preferred embodiment, fluid infusion mouth is additionally provided with feed pipe and tank interior
Connection, the length of described feed pipe is as the criterion with the liquid level that can stretch to tank interior, and during to prevent from feeding, liquid surface fluctuation is to hairy
The destruction of root, and prevent pipeline from exposing, it is to avoid external source is polluted.
The hairy root liquid culture device of the present invention is transparent high temperature resistant material, with metal materials such as rustless steels in prior art
Compare, can the growth conditions of hairy root in real-time monitored tank body；Gas generating unit is placed in tank base, and arranges anchor
Groove, it is achieved top-blown oxygen supply, and given vent to anger by the filter membrane being arranged on seal closure, compared to traditional, blow vent is located at cultivation
Design bottom device, can prevent the fluid seepage that bottom opening causes, and effectively prevent exogenous pollution；Each junction in tank body
All use arc, it is ensured that without dead angle in tank body, impurities left in incubator can be prevented, also can reduce fluid vortex simultaneously, stable
Growth of hair root.
Accompanying drawing explanation
Accompanying drawing 1 width of the present invention:
Fig. 1 is the hairy root incubator schematic diagram of the present invention,
Wherein 1. tank body, 2. gas generating unit, 3. breather, 4. BULKHEAD UNION, 5. filter, 11. tank mouths, 12. envelopes
Lid, 13. sealers, 14. air inlets, 15. streams add fluid infusion mouth, 16. grooves.
Detailed description of the invention
Specific examples below is for the present invention is further illustrated, should not be construed as the present invention any form of
Limit.
Embodiment 1
The hairy root liquid culture device of the present invention, as it is shown in figure 1, include tank body 1, tank body 1 top is the tank mouth 11 shunk,
Tank mouth 11 is provided with seal closure 12, the described centrally disposed sealer of seal closure 12 13, and described sealer is one-way ventilating and impermeable
The micropore sterilised membrane filter of bacterium；Being respectively equipped with air inlet 14 on two shouldered walls of tank body 1 and stream adds fluid infusion mouth 15, tank base is arranged
Having a groove 16, fixed gas generating means 2 in described groove 16, gas generating unit 2 is by air inlet pipe 3 and air inlet 14
Connect.Described air inlet pipe 3 is hard tube, is cased with one layer of rubber sleeve flexible pipe outside it；BULKHEAD UNION, tank body are installed at air inlet 14
It is also associated with filter 5 in outside air inlet pipe 3.Described groove 16 is circular arc, is arranged at the center of tank base, tank mouth 11
It is arc is connected design with tank body wall, tank body wall and tank base, tank body 1 Shang Ge corner.The material of described tank body 1
For food-grade transparent PC plastics.The width of the incubator tank mouth 11 of the present invention and the width ratio of tank body 1 main body are 4:9, at the bottom of tank body
The total length ratio of the projection in portion and the aggregate level length of female and tank base is 1:3.
When the incubator using the present invention carries out the cultivation of hairy root, ventilation and stream add formula fluid infusion all to incubator inner loop
Border impact is less, and without eddy current in incubator, free from admixture remains, and dissolved oxygen is uniform, and without external source pollution problem, interior hairy root can realize
The most stable growth.
Claims (9)
1. a hairy root liquid culture device, including tank body (1), tank body (1) top is the tank mouth (11) shunk, on tank mouth (11)
It is provided with seal closure (12), seal closure (12) centrally disposed sealer (13)；It is respectively equipped with air inlet on two shouldered walls of tank body (1)
(14) and stream adds fluid infusion mouth (15), tank base is provided centrally with a groove (16), and described groove (16) interior fixed gas is sent out
Generating apparatus (2), gas generating unit (2) is connected with air inlet (14) by air inlet pipe (3).
Incubator the most according to claim 1, it is characterised in that described air inlet pipe (3) is hard tube, is cased with one layer outside it
Rubber sleeve.
Incubator the most according to claim 1, it is characterised in that described sealer (13) is one-way ventilating film.
Incubator the most according to claim 3, it is characterised in that described one-way ventilating film be ventilative impermeable bacterium micropore without
Bacterium filter membrane.
Incubator the most according to claim 1, it is characterised in that described air inlet (14) place is provided with BULKHEAD UNION, tank
It is also associated with filter (5) in the air inlet pipe (3) of external body.
Incubator the most according to claim 1, it is characterised in that described tank mouth (11) and tank body wall, tank body wall with
Tank base, the groove (16) of tank base and tank body (1) Shang Ge corner are arc and connect design.
Incubator the most according to claim 1, it is characterised in that the material of described tank body (1) is transparent high temperature resistant material.
Incubator the most according to claim 1, it is characterised in that the width of described tank mouth (11) and tank body (1) main body
Width ratio is 1:2～3.
Incubator the most according to claim 1, it is characterised in that the projection of tank base and the aggregate level of female are long
Degree is 1:2～4 with the total length ratio of tank base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610615411.0A CN106085853A (en) | 2016-07-31 | 2016-07-31 | Hairy root liquid culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610615411.0A CN106085853A (en) | 2016-07-31 | 2016-07-31 | Hairy root liquid culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106085853A true CN106085853A (en) | 2016-11-09 |
Family
ID=57479566
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610615411.0A Pending CN106085853A (en) | 2016-07-31 | 2016-07-31 | Hairy root liquid culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106085853A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1381561A (en) * | 2001-04-13 | 2002-11-27 | 上海市中药研究所 | Culture device made of soft material |
| CN102783414A (en) * | 2011-05-19 | 2012-11-21 | 河南省海乐森药用细胞工程技术有限公司 | External circulation mist-type bioreactor for large-scale cultivation of plant hairy roots |
| CN205099687U (en) * | 2015-09-25 | 2016-03-23 | 浙江理工大学 | Special bioreactor of hair form root |
| CN205907269U (en) * | 2016-07-31 | 2017-01-25 | 大连德聚盛生态科技有限公司 | Hair form root liquid culture plate |
-
2016
- 2016-07-31 CN CN201610615411.0A patent/CN106085853A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1381561A (en) * | 2001-04-13 | 2002-11-27 | 上海市中药研究所 | Culture device made of soft material |
| CN102783414A (en) * | 2011-05-19 | 2012-11-21 | 河南省海乐森药用细胞工程技术有限公司 | External circulation mist-type bioreactor for large-scale cultivation of plant hairy roots |
| CN205099687U (en) * | 2015-09-25 | 2016-03-23 | 浙江理工大学 | Special bioreactor of hair form root |
| CN205907269U (en) * | 2016-07-31 | 2017-01-25 | 大连德聚盛生态科技有限公司 | Hair form root liquid culture plate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101555455B (en) | Culture/harvesting method of microalgae and system | |
| US20170145361A1 (en) | Bioreactor with separate co2 supply | |
| US20160177248A1 (en) | Pivoting pressurized single use bioreactor | |
| CN213073934U (en) | Plant sugar-free culture system and plant culture container | |
| CN205295351U (en) | Pipeline algae photobioreactor that declines | |
| CN103224882B (en) | Disposable wave type bioreactor with aerating apparatus | |
| CN110622860B (en) | Plant tissue high-efficiency culture equipment | |
| CN103255046A (en) | Circulation foam photobioreactor and application thereof | |
| CN205907269U (en) | Hair form root liquid culture plate | |
| CN207109008U (en) | A kind of multifunctional microbial incubator | |
| CN102911856B (en) | Tangential casing built-in aeration photo-bioreactor applicable to micro algae high-efficiency culture | |
| CN205902523U (en) | A gaseous conveyor for plant cultivation room | |
| CN208362332U (en) | A kind of animal nutrition fermentor | |
| CN102242064B (en) | Continuous air supplying method and system for chlamydomonas cultivation | |
| CN106085853A (en) | Hairy root liquid culture device | |
| KR101654559B1 (en) | Cell culture apparatus | |
| CN206843447U (en) | A kind of gravity flow photosynthetic reaction device | |
| CN109251847A (en) | Utilize the device and method of sunlight culture photosynthetic microorganism | |
| RU2585666C1 (en) | Device for cultivation of methane-oxidising microorganisms | |
| CN203238251U (en) | Microalgae culture pond with novel aeration device | |
| CN206266628U (en) | Culture device for liquid strain of edible fungus | |
| RU2644344C1 (en) | Biological reactor for transforming gas-hydrogen hydrocarbons to biologically active compounds | |
| AU2012379589B2 (en) | Trap type carbon replenishing device for culturing microalgae of opened pool and carbon replenishing method thereof | |
| CN206408219U (en) | A kind of fermentation tank oxygen supplementing device | |
| CN204162726U (en) | The biological complete device for producing of cell cultures |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161109 |