CN106074617B - A kind of propolis extract and its extraction process - Google Patents
A kind of propolis extract and its extraction process Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
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- A61K35/64—Insects, e.g. bees, wasps or fleas
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Abstract
The application belongs to propolis extractive technique field, and in particular to a kind of propolis extract and its extraction process.The extraction process, comprises the following steps：1) propolis raw material is taken, picks impurity, is crushed after being freezed under the conditions of 10 DEG C~20 DEG C, fine powder is obtained after sieving, it is standby；2) mixed solvent is added into fine powder, is ultrasonically treated 30 60 minutes under the conditions of 15 35 DEG C, is then filtered, obtain filter residue and filtrate, the filtrate that is concentrated under reduced pressure obtains solid 1；3) ethanol water is added into filter residue obtained by step 2) and is extracted 13 times at 65 75 DEG C, 1 3h, extract solution is filtered every time, obtains filtrate；4) gained solid 2 is drying to obtain propolis extract after merging with solid 1 after the filtrate decompression is concentrated.The extraction process provided by the application extracts obtained Flavonoids in Propolis, the content height of volatile oil, and impurity (such as lead) content is few.
Description
Technical field
The invention belongs to the extraction and separation technology field of propolis, and in particular to a kind of propolis extract and its extraction process.
Background technology
Propolis is apis mellifera herborization resin, and is mixed into after beeswax, pollen and honeybee glandular secretion thing through honeybee nozzle
A kind of thick substances being process are chewed, there is pleasant aromatic odor.Honeybee fills up honeycomb space, smooth honeybee using propolis
Nest inwall, and prevent infection rotted with germ of foreign matter etc. in lair using propolis.
Propolis complicated component, about by 55% resin and tree perfume, 30% or so beeswax, 10% fragrant volatile oil and
5% pollen and debris composition.Because collagen plant is different, there is very big difference in the chemical composition for producing propolis for different regions.
Propolis has the quality specificity that the hard crisp, high temperature of low temperature becomes sticky, not soluble in water, is partially soluble in the organic solvents such as ethanol, therefore
Propolis need to be processed using during propolis.
In Europe and the Central Asia, propolis has a long applicating history as a kind of folk medicine, to honeybee in China's historical document
The application and production of glue are recorded few.In recent years, because propolis in the special nature of pharmacology and chemical field, propolis turns into state
One big focus of inside and outside research and development.《Pharmacopoeia of People's Republic of China》Propolis is included wherein in versions first in 2005.From
Pharmacy angle sees that propolis is the Chinese medicine of animal and plant double source simply；Chemically seen into subangle, propolis contain more than 20 classes,
More than 300 kinds of natural component, there is the complexity and changeability of height.In terms of biological activity angle, propolis have broad-spectrum antiseptic,
The quite varied biological actions such as anti-oxidant, antitumor, regulation blood-fat and blood sugar.
At present the own principal component in propolis include Polyphenols (flavones and phenolic acid), alcohol, alkene, aldehyde, ester, ether, mushroom class, steroid,
Sugar, amino acid, aliphatic acid and trace element.The maximum feature of propolis is containing abundant flavone compound, there is " flavonoids
The title of the treasure-house of compound ".Flavone compound in propolis has antibacterial, antivirus, anti-inflammatory, analgesic, reducing blood lipid, promotion organization again
Raw and enhancement body immunity and other effects.
In the prior art the extraction to propolis farthest extracts polyphenols primarily directed to Polyphenols,
In fact, the pharmacological action of propolis is the result that the collaboration of its various active composition works.But volatile materials and vitamin etc.
Nutritional ingredient yield is extremely low.Volatile ingredient contained by propolis predominantly accounts for 10% fragrant volatile oil in propolis.External suppression
Bacterium experiment shows propolis volatile materials to grampostive bacteria:Staphylococcus aureus, MRSE and Gram are cloudy
Property bacterium:The bacteriums such as Pseudomonas aeruginosa, Escherichia coli, Friedlander's bacillus, enterobacter cloacae and Candida albicans, torrid zone vacation
The fungies such as silk yeast, Candida glabrata have inhibitory action.Also scientist, which studies, finds that propolis volatile materials is stored in rice
During to rice lipid peroxidation and hydrolysis it is inhibited.But it is different from the plant conventionally used for volatile oil extracting in propolis
Thing tissue, there is the hard quality specificity crisp, high temperature is sticky of low temperature.
Through retrieval, the patent related to propolis extraction process has following several：
1) Chinese patent 201210227678.4《The method of propolis extraction》Disclose a kind of method of propolis extraction.The party
Method is combined with ultrasonic cavitation technology with different concentration ethanol extraction propolis technology, and technique is simple, and process parameters range is accurate, carries
The high flavones content of propolis, lead content is reduced, improve propolis quality.But this method is low to the recovery rate of volatile oil.
2) Chinese patent 201110117989.0《A kind of extraction process of propolis》Disclose a kind of extraction process of propolis.
This method utilizes edible alcohol soaking at room temperature propolis raw material, is obtained after then filtered, vacuum concentration.The propolis that the invention is related to
Purifying technique, propolis purification rate is high, and loss of volatile oil is few, and active ingredient is more after extraction, and the letter of the step of whole purifying technique
It is single, save the time, purification efficiency is high.But the lead content that extraction obtains propolis is high, and propolis quality is low.
3) Chinese patent 201310473370.2《A kind of propolis extract and its application》Disclose a kind of using different dense
The method of ethanol extraction and cleaning propolis is spent, the extraction species that this method obtains have anti-inflammatory, hepatoprotective effect, to human leukocytes elasticity
Protease is inhibited；Cause hepatic injury that there is protective effect in carbon tetrachloride.It is but main in the extract that this method obtains
It is flavonoids to want active ingredient.
The extraction to propolis at present is can be seen that mainly using ethanol as solvent from published patent above, carries out room
The nytron such as temperature immersion or refluxing extraction, but according to the similar principle that mixes, the composition terpene of the low pole in volatile oil, alkenes
Thing dissolubility in highly polar ethanol (or ethanol water) is smaller, so, the extraction of published propolis in above-mentioned patent
It is extremely low that the content of the volatile oil in obtained propolis is extracted in method, the needs of actual production can not be met.
The content of the invention
In order to solve above mentioned problem of the prior art, the application aims to provide a kind of propolis extraction process, so as to get
Propolis flavone, the content of volatile oil are high, while impurity (such as lead) content is few.
In order to realize foregoing invention purpose, this application provides a kind of propolis extraction process, and it comprises the following steps：
1) propolis raw material is taken, picks impurity, 8~12h is freezed under the conditions of -10 DEG C~-20 DEG C；Then crush, excessively 80~
Fine powder is obtained after 100 mesh sieves, it is standby；
2) fine powder is pressed into fine powder:Mixed solvent=1:(8~22) W/V ratio adds mixed solvent, 15~35 DEG C of bars
It is ultrasonically treated 30~60 minutes under part, then filters, obtain filter residue and filtrate, the filtrate that is concentrated under reduced pressure obtains solid 1；
Wherein, mixed solvent is n-hexane:Ethyl acetate:95% ethanol water=(1~6):1:(0.01~0.1) V/
V/V；
3) filter residue is pressed into filter residue obtained by step 2):75% ethanol water=1:(4~8) W/V ratios add ethanol water
Solution, and extracted 1~3 time at 65~75 DEG C, 1~3h, extract solution is filtered every time, obtains filtrate；
4) gained solid 2 is drying to obtain propolis extract after merging with solid 1 after the filtrate decompression is concentrated.
Preferably, step 1) operation is as follows：Take propolis raw material, pick impurity, under the conditions of -10 DEG C~-20 DEG C freeze 2~
4~36cm is ground into after 4h3The bulk of size；Continue to crush after freezing 1~3h at -10 DEG C~-20 DEG C, cross 20~60 mesh sieves
Coarse powder is obtained afterwards；Fine powder is obtained after 80~100 mesh sieves are finally crossed after 1~9h of freezing under the conditions of -10 DEG C~-20 DEG C, it is standby.Divide more
Secondary, gradient fragmentation propolis raw material, the propolis plasticizing caused by temperature rise in crushing process can be avoided and adhere to each other or glue
The loss that propolis raw material is caused on crusher is attached to, wherein to be optimal three times, had both improved the extraction effect of whole extraction process
Rate, the energy consumption in crushing process can also be reduced.
Preferably, step 1) operation is as follows：Take propolis raw material, pick impurity, under the conditions of -10 DEG C~-20 DEG C freeze 8~
12h；Crushed after adding the crushing medium equivalent to the weight % of propolis raw material 3~8 thereto, fine powder obtained after crossing 80~100 mesh sieves,
It is standby.
Crushing medium is added in propolis raw material can also avoid the propolis caused by temperature rise in crushing process from plastifying
And adhere to each other or be adhered on crusher.
Completed it is further preferred that described crushing and sieving will control at 20~30 minutes；Described crushing medium is
Rice husk, rice bran or wheat bran.
It is further preferred that the pharmacy equivalent to the crushing medium 1~5 weight % is added into described crushing medium
Upper acceptable lubricant, such as talcum powder, calcium carbonate, magnesium stearate.
Preferably, in step 3), when carrying out ethanol extraction, it has been additionally added equivalent to the weight % of propolis raw material 0.3~0.8
Natural surfactant, it is further preferred that the natural surfactant is lecithin, such as soybean lecithin.In ethanol
Natural surfactant is added during extraction, the dissolution of water soluble ingredient in propolis can be improved, and then active ingredient can be improved
Extraction efficiency.
The propolis extract for extracting to obtain by above-mentioned propolis extraction process, wherein, flavones content 22-30%, volatile oil
Content is 7-9.5%.
Compared with prior art, the propolis extraction process that the application provides has advantages below：
1) several times, gradient fragmentation propolis raw material, can avoid because in crushing process temperature rise caused by propolis plastify
And the loss that propolis raw material is caused on crusher is adhered to each other or is adhered to, wherein to be optimal three times, both improved and entirely carried
The extraction efficiency of taking technique, the energy consumption in crushing process can also be reduced.
2) according to the similar principle that mixes, the mixed solvent formed using opposed polarity solvent is first carried the application to propolis
Take, then extracted again with ethanol, flavones content can be extremely to the content of 30%, volatile oil in the propolis extract for extracting to obtain
9.5%, while impurity (such as lead) content is few.
Embodiment
The present invention is further elaborated with reference to embodiment.These embodiments are only in order at purpose of explanation,
And do not limit the scope of the invention and essence.Unless otherwise specified, the operation of the application uses the routine of those skilled in the art
Means are carried out, and are such as filtered, using being filtered under diminished pressure；Dry, then carry out under vacuum.Used solvent is pure to analyze.Honeybee
Collagen material is purchased from Daxing district, Beijing, China.
Unless otherwise specified, the percentage in the present invention is weight percentage；The concentration of ethanol water is volume
Percent concentration.
Embodiment 1
A kind of propolis extraction process, it comprises the following steps：
1) propolis raw material is taken, picks impurity, 10h is freezed under the conditions of -20 DEG C；Then crush, obtained carefully after crossing 100 mesh sieves
Powder, it is standby；
2) fine powder is pressed into fine powder:Mixed solvent=1:22W/V ratio adds mixed solvent, under the conditions of 30 DEG C at ultrasound
Reason 60 minutes, then filters, obtains filter residue and filtrate, the filtrate that is concentrated under reduced pressure obtains solid 1；
Wherein, mixed solvent is n-hexane:Ethyl acetate:95% ethanol water=1:1:0.1V/V/V；
3) filter residue is pressed into filter residue obtained by step 2):75% ethanol water=1:5W/V ratios add ethanol water simultaneously
Extracted 2 times at 65 DEG C, each 2h, extract solution is filtered, obtains filtrate；
4) gained solid 2 is drying to obtain propolis extract after merging with solid 1 after the filtrate decompression is concentrated.
Embodiment 2
A kind of propolis extraction process, it comprises the following steps：
1) 1 kilogram of propolis raw material is taken, picks impurity, 6h is freezed under the conditions of -20 DEG C, crushes, continues under the conditions of -20 DEG C
6h is freezed, is crushed, fine powder is obtained after crossing 100 mesh sieves, it is standby；
2) fine powder is pressed into fine powder:Mixed solvent=1:22W/V ratio adds mixed solvent, under the conditions of 30 DEG C at ultrasound
Reason 60 minutes, then filters, obtains filter residue and filtrate, the filtrate that is concentrated under reduced pressure obtains solid 1；
Wherein, mixed solvent is n-hexane:Ethyl acetate:95% ethanol water=1:1:0.1V/V/V；
3) filter residue is pressed into filter residue obtained by step 2):75% ethanol water=1:5W/V ratios add ethanol water simultaneously
Extracted 2 times at 65 DEG C, each 2h, extract solution is filtered, obtains filtrate；
4) gained solid 2 is drying to obtain propolis extract after merging with solid 1 after the filtrate decompression is concentrated.
Wherein, measure the present embodiment to extract to obtain 952 grams of propolis extract, with reference to GB/T24283-2009《Propolis》In it is total
It is 22% that the assay method of flavones, which measures and originally applies the contents of Flavonoid substances in the propolis extract that example is extracted to obtain, using GC-
The content that MS measures volatile ingredient in the extract is 7%.
Embodiment 3
A kind of propolis extraction process, it comprises the following steps：
1) 1 kilogram of propolis raw material is taken, picks impurity, 25cm is ground into after freezing 4h under the conditions of -20 DEG C3The block of size
Shape；Continue to crush after freezing 2h at -20 DEG C, coarse powder is obtained after crossing 20 mesh sieves；It crushed after finally freezing 4h under the conditions of -20 DEG C
Fine powder is obtained after 80 mesh sieves, it is standby.
2) fine powder is pressed into fine powder:Mixed solvent=1:22W/V ratio adds mixed solvent, under the conditions of 30 DEG C at ultrasound
Reason 60 minutes, then filters, obtains filter residue and filtrate, the filtrate that is concentrated under reduced pressure obtains solid 1；
Wherein, mixed solvent is n-hexane:Ethyl acetate:95% ethanol water=1:1:0.1V/V/V；
3) 3 grams of soybean lecithin is added into filter residue obtained by step 2), then by filter residue:75% ethanol water=1:
5W/V ratios add ethanol water and extracted 2 times at 65 DEG C, each 2h, extract solution are filtered, obtains filtrate；
4) gained solid 2 is drying to obtain propolis extract after merging with solid 1 after the filtrate decompression is concentrated.
966 grams of the propolis extract that the present embodiment extracts to obtain is measured, propolis extraction is measured using the method for same embodiment 2
The content of Flavonoid substances is 25% in thing, uses GC-MS to measure the content of volatile ingredient in the extract as 9%.
Embodiment 4
A kind of propolis extraction process, it comprises the following steps：
1) 1 kilogram of propolis raw material is taken, impurity is picked, 8h is freezed under the conditions of -10 DEG C；50 grams of rice husk is added thereto,
Crush, fine powder is obtained after crossing 100 mesh sieves, it is standby.
2) fine powder is pressed into fine powder:Mixed solvent=1:22W/V ratio adds mixed solvent, under the conditions of 30 DEG C at ultrasound
Reason 60 minutes, then filters, obtains filter residue and filtrate, the filtrate that is concentrated under reduced pressure obtains solid 1；
3) 5 grams of soybean lecithin is added into filter residue obtained by step 2), then by filter residue:75% ethanol water=1:
5W/V ratios add ethanol water and extracted 3 times at 65 DEG C, each 3h, extract solution are filtered, obtains filtrate；
4) gained solid 2 is drying to obtain propolis extract after merging with solid 1 after the filtrate decompression is concentrated.
Originally 963 grams of the propolis extract that example is extracted to obtain is applied, is measured using the method for same embodiment 2 yellow in propolis extract
The content of letones is 24%, uses GC-MS to measure the content of volatile ingredient in the extract as 7.8%.
Embodiment 5
A kind of propolis extraction process, it comprises the following steps：
1) 1 kilogram of propolis raw material is taken, picks impurity, 50 grams of rice husk and 0.5 gram of talcum powder are added thereto, -15
36cm is ground into after freezing 2h under the conditions of DEG C3The bulk of size；Continue to crush after freezing 2h at -15 DEG C, obtained after crossing 20 mesh sieves
Coarse powder；Fine powder is obtained after 80 mesh sieves are finally crushed after freezing 8h under the conditions of -15 DEG C, it is standby；
2) fine powder is pressed into fine powder:Mixed solvent=1:12W/V ratio adds mixed solvent, under the conditions of 30 DEG C at ultrasound
Reason 60 minutes, then filters, obtains filter residue and filtrate, the filtrate that is concentrated under reduced pressure obtains solid 1；
Wherein, mixed solvent is n-hexane:Ethyl acetate:95% ethanol water=6:1:0.01V/V/V；
3) 5 grams of soybean lecithin is added into filter residue obtained by step 2), then by filter residue:75% ethanol water=1:
5W/V ratios add ethanol water and extracted 3 times at 65 DEG C, each 3h, extract solution are filtered, obtains filtrate；
4) gained solid 2 is drying to obtain propolis extract after merging with solid 1 after the filtrate decompression is concentrated.
Originally 970 grams of the propolis extract that example is extracted to obtain is applied, is measured using the method for same embodiment 2 yellow in propolis extract
The content of letones is 28%, uses GC-MS to measure the content of volatile ingredient in the extract as 9.3%.
Comparative example 1
A kind of propolis extraction process, it comprises the following steps：
1) 1 kilogram of propolis raw material is freezed 12 hours under -10 DEG C of liquid nitrogen environment, then crushed, will first freezed
Block glue is broken into 5-10mm fritter, and 300 targeted fine powders are then ground into disintegrating machine, standby；
2) the 3-4 times of edible alcohol weighed is added into fine powder, the concentration of alcohol is 75%, is soaked 7 days, every in immersion process
20-30 minutes were stirred every 2 hours；
3) first with the filter cloth coarse filtration of 100 mesh, filtrate is subjected to refined filtration after 24 hours in 0 DEG C of refrigeration house storage, with 400 during refined filtration
Purpose filter cloth, obtained filtrate is stand-by, wherein, the filter residue remained after coarse filtration carries out refined filtration again after adding edible alcohol, obtains
Filtrate is also added thereto stand-by；
4) propolis ointment is obtained through vacuum-concentrcted, wherein vacuum degree control passes through in below 0.08MPa, the alcohol of evaporation
Reused after recovery.
This comparative example extracts 937 grams obtained of propolis extract, is measured using the method for same embodiment 2 in propolis extract
The content of Flavonoid substances is 20%, uses GC-MS to measure the content of volatile ingredient in the extract as 5%.
Comparative example 2
A kind of method of propolis extraction, it comprises the following steps：
1) freezed 24 hours in the environment of 1 kilogram of propolis raw material being placed in into -4~-8 DEG C；
2) the hair glue after freezing, it is aided with 10% crushing medium, gained hair glue particle, immediately enters reciprocating sieve；
3) the hair glue particle by grain fineness after screening for 40~60 mesh, by 82% containing the charge ratio with ethanol and hair glue
4:After 1 (solid-to-liquid ratio) dispensing, extracted 48 hours at 25~30 DEG C, 30min, and positive and negative alternate, extracted were stirred every 1 hour
It is aided with ultrasonic enhanced extraction in journey, ultrasonic 1 time every 6 hours, each ultrasonic time 30min, ultrasonic power 2000W, 48 is small
When after filter supernatant for the first time and be pumped into and settled with cooling coil in jar, then charge ratio 2 pressed with 75% edible ethanol:1
After (solid-to-liquid ratio) dispensing, extracted 24 hours at 25~30 DEG C, 20min, and positive and negative alternate, leaching process were stirred every 1 hour
In be aided with ultrasonic enhanced extraction, every 12 hours ultrasounds 1 time, each ultrasonic time 20min, ultrasonic power 1000W, rear second
Secondary suction filtration supernatant is pumped into be settled in jar with cooling coil, presses charge ratio 1 again with 90% edible ethanol:1 (solid-liquid
Than) after dispensing, extracted 24 hours at 25~30 DEG C, 10min was stirred every 1 hour, and it is positive and negative alternate, it is aided with leaching process
Ultrasonic enhanced extraction, every 12 hours ultrasounds 1 time, each ultrasonic time 10min, ultrasonic power 800W, rear third time filtered
Supernatant is pumped into be settled in jar with cooling coil.After supernatant exhausts three times, by remaining Liquid Residue together with residue
With adding the centrifuge of three layers of 80-120 mesh filter clothes to dry, filtrate is taken to be incorporated to above-mentioned settling tank after filtering, with air-flow mixing homogeneous
It is to be concentrated in -4~-0 DEG C of cooling-sedimentation more than 48 hours, plate-frame filtering of the sedimentation liquid again through 500 mesh filter clothes, filtrate again afterwards.Treat
Concentration filtrate point enters decompression evaporator several times, 300 liters every time, using heating water bath.40~50 DEG C of thickening temperature, vacuum
More than 0.096MPa, produces propolis after concentration, after casting propolis medicinal extract.
This comparative example extracts 935 grams obtained of propolis extract, is measured using the method for same embodiment 2 in propolis extract
The content of Flavonoid substances is 24%, uses GC-MS to measure the content of volatile ingredient in the extract as 4.7%.
Above-described embodiment is only not limited as the explanation purpose of the present invention, the scope of the present invention.To the skill of this area
The modification made for art personnel is it will be apparent that the present invention is only limited by scope.
Claims (6)
1. a kind of propolis extraction process, it comprises the following steps：
Step 1）Operation is as follows：Propolis raw material is taken, picks impurity, 8~12h is freezed under the conditions of -10 DEG C~-20 DEG C；Thereto
Crushed after adding the crushing medium equivalent to the weight % of propolis raw material 3~8, fine powder is obtained after crossing 80-100 mesh sieves, it is standby；Described
Crushing and sieving will control to be completed at 20~30 minutes；Described crushing medium is rice husk, rice bran or wheat bran；
2）Fine powder is pressed into fine powder:Mixed solvent=1:（8~22）W/V ratio addition mixed solvent, 15~35oSurpass under the conditions of C
Sonication 30~60 minutes, then filters, obtains filter residue and filtrate, and the filtrate that is concentrated under reduced pressure obtains solid 1；
Wherein, mixed solvent is n-hexane:Ethyl acetate:95% ethanol water=（1~6）:1:（0.01~0.1） V/V/V；
3）To step 2）Filter residue is pressed in gained filter residue:75% ethanol water=1:（4~8）W/V ratios add ethanol water, and
In 65~75oExtracted 1~3 time under C, 1~3h, extract solution is filtered every time, obtains filtrate；
4）Gained solid 2 is drying to obtain propolis extract after merging with solid 1 after the filtrate decompression is concentrated；
Step 3）In, when carrying out ethanol extraction, it is additionally added the self-faced equivalent to the weight % of propolis raw material 0.3~0.8 and lives
Property agent.
2. extraction process according to claim 1, wherein, added into described crushing medium equivalent to the crushing medium
1-5 weight % pharmaceutically acceptable lubricant.
3. extraction process according to claim 2, described lubricant is talcum powder, calcium carbonate, magnesium stearate.
4. extraction process according to claim 1, described natural surfactant is lecithin.
5. a kind of propolis extract, it is to extract to obtain as the extraction process described in claim any one of 1-4.
6. propolis extract according to claim 5, wherein, flavones content 22-30%, volatile oil content 7-9.5%.
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| CN107028984B (en) * | 2016-11-17 | 2020-08-04 | 南京希元生物医药科技有限公司 | Propolis gel, propolis gel lyophilized powder, and preparation process and application thereof |
| CN109601936A (en) * | 2018-12-28 | 2019-04-12 | 江苏鸿祺生物科技有限公司 | A kind of propolis extract and its health-care food composition preventing senile dementia |
| CN109567188A (en) * | 2018-12-28 | 2019-04-05 | 江苏鸿祺生物科技有限公司 | A kind of health-care food composition and its preparation method and application of propolis-containing extract |
| CN110464696A (en) * | 2019-09-18 | 2019-11-19 | 北京蜜慕科技有限公司 | A kind of preparation method and applications of small molecule propolis extract |
| CN112057477A (en) * | 2020-09-30 | 2020-12-11 | 江苏江大源生态生物科技股份有限公司 | Method for preparing propolis Chinese medicinal pill |
| CN113926218B (en) * | 2021-11-15 | 2022-12-06 | 老蜂农生物科技集团有限公司 | Production process of propolis ethanol extract and propolis ethanol extract |
| CN114306390A (en) * | 2022-01-23 | 2022-04-12 | 吉林蜂道馆健康产业有限公司 | Preparation method of bee therapy product for reducing blood sugar |
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| CN114748512A (en) * | 2022-04-06 | 2022-07-15 | 江苏蜂奥生物科技有限公司 | Method for rapidly extracting propolis |
| CN115138099B (en) * | 2022-08-24 | 2024-05-28 | 云南润嘉药业有限公司 | A method for preparing propolis extract |
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| 蜂胶挥发油成分比较;杨文超等;《中国蜂业》;20120331;第63卷;01～03月中旬刊:第58页 * |
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