CN106071053A - A kind of efficiently Synbiotic feed additive - Google Patents
A kind of efficiently Synbiotic feed additive Download PDFInfo
- Publication number
- CN106071053A CN106071053A CN201610515946.0A CN201610515946A CN106071053A CN 106071053 A CN106071053 A CN 106071053A CN 201610515946 A CN201610515946 A CN 201610515946A CN 106071053 A CN106071053 A CN 106071053A
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- CN
- China
- Prior art keywords
- clostridium butyricum
- polysaccharide
- feed additive
- mass percent
- saccharomyces cerevisiae
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a kind of efficiently Synbiotic feed additive, composition mass percent is: 45～the probiotic bacteria of 50% and 50～the prebiotics of 55%, and wherein probiotic bacteria composition mass percent is: clostridium butyricum 30～40%, lactic acid bacillus 45～55%, Bafillus natt 10～20%；Described prebiotics composition mass percent is: Analysis of Polysaccharide From Saccharomyces Cerevisiae 60～70%, sulphation Analysis of Polysaccharide From Saccharomyces Cerevisiae 30～40%.Substitute antibiotics of the present invention cultivation growing and fattening pigs, feedstuff-meat ratio reaches 1.7:1, and daily gain reaches 730 900g, delivers natural law for sale and shifts to an earlier date 10 days, and dressing percentage is more than 70%, improves immunity and the premunition of live pig simultaneously, and economic benefit is notable with social benefit；Stable processing technique, technology maturation, it is suitable for large-scale production and promotes.
Description
Technical field
The present invention relates to a kind of feed additive, particularly relate to a kind of efficiently Synbiotic feed additive.
Background technology
Antibiotic plays great function in animal productiong the most always, can effectively treat and prevent animal disease
Sick generation, but the lasting use of antibiotic can cause the problems such as bacterial drug resistance and animal product drug residue, seeks
Become present feed industry with exploitation feed additive substitute antibiotics feed additive green, pollution-free, noresidue to grind
The focus studied carefully.
But, existing technology uses medicinal herb components in a large number, this additive relies primarily on the pre-of Chinese herbal medicine
Anti-and treatment disease ability substitutes existing antibiotic, but actually used be still to there is persistently use to cause bacterial resistance
The problems such as property and drug residue, and its cost increases considerably, and does not meet market discipline, hence without being pushed away
Wide application.
Summary of the invention
Present invention aims to deficiency of the prior art, it is provided that a kind of efficiently Synbiotic feed additive, be
A kind of green feed additive not using antibiotic, is suitable for large-scale promotion.
For completing above-mentioned purpose, the technical solution used in the present invention is: a kind of efficiently Synbiotic feed additive, its composition
Mass percent is: 45～the probiotic bacteria of 50% and 50～the prebiotics of 55%, and wherein probiotic bacteria composition mass percent is: butanoic acid
Clostridium 30～40%, lactic acid bacillus 45～55%, Bafillus natt 10～20%；Described prebiotics composition matter
Amount percentage ratio is: Analysis of Polysaccharide From Saccharomyces Cerevisiae 60～70%, sulphation Analysis of Polysaccharide From Saccharomyces Cerevisiae 30～40%.
Further, the total plate count 1.6 × 10 of clostridium butyricum in described probiotic bacteria9～1.9 × 109 cfu/
G, the total plate count 6 × 10 of lactic acid bacillus8～9 × 108Cfu/g, the total plate count 1 × 10 of Bafillus natt9～2 ×
109 cfu/g。
Further, the preparation method of described clostridium butyricum is: takes strain and is inoculated in butanoic acid shuttle shape spore bar
In bacterium culture medium, 36～38 DEG C of constant temperature stand, and Anaerobic culturel 20～24h obtains first order seed bacterium, first order seed bacterium is inoculated into fourth
In acid clostridium culture medium, obtain secondary seed bacterium solution, wherein first order seed bacterium and clostridium butyricum culture medium
Mass ratio is 1:15～24；Take clostridium butyricum culture medium dilute 19～21 times, go out in 110～120 DEG C of steam
Bacterium 20～30min, is cooled to 35～40 DEG C, add secondary seed bacterium solution, stir, constant temperature 35～45 DEG C of fermentation culture 32～
The volume ratio of the clostridium butyricum kind culture medium after 40h, added secondary seed bacterium solution and dilution is 1:7～12；Fermentation
After completing, take fermentation liquid and be cooled to room temperature, then 2500～3000r/min be centrifuged 15～20min, remove supernatant, use 20-30%
Lac Bovis seu Bubali juice as protective agent, bacterium mud is washed out, then it is mixed with the liquid that is coated containing gelatin, homogenizing is coated, freezing dry
Dry, grind into powder.
Further, the composition weight portion of described clostridium butyricum culture medium is: yeast extract 5, peptone 10,
Glucose 10, beef extract 3, ammonium sulfate 1, NaCl 2, dipotassium hydrogen phosphate 4, sodium bicarbonate 1, MnSO4 0.2、 MgSO4
7H2O 0.5、 CaCO31, distilled water 1000.
Further, the preparation method of described Analysis of Polysaccharide From Saccharomyces Cerevisiae is: take beer yeast slurry, cleans with distilled water, vacuum
Sucking filtration, washes the most again, after placing 0.5～1 h, outwells supernatant, centrifugal, adds the 0.5% of twice beer yeast slurry volume
Sodium bicarbonate washing 1～2 h, then wash twice, centrifugal, abandon supernatant and obtain clean beer yeast, roll over according to its water content
It is counted as dry yeast weight, adds appropriate water and Sal makes yeast and brine concentration be 8～10%, solution is placed in high pressure equal
In matter machine, at temperature 110～120 DEG C, low pressure 15MPa, each homogenizing 3 times under conditions of high pressure 65MPa, cooling, high speed centrifugation, protect
Stay supernatant, be concentrated into the 1/4～1/5 of original volume, make precipitant with 95% ethanol, precipitate 10～12h, ethanol and supernatant
Volume ratio is 2～3:1, precipitation washing with acetone 2 times, washs 1 time with ether, finally will be deposited at 30～40 DEG C dry 12～
16h, to obtain final product.
Further, the preparation method of described sulphation Analysis of Polysaccharide From Saccharomyces Cerevisiae is: in mass ratio 2.0～4.0:1 weigh three
Sulfur oxide and Analysis of Polysaccharide From Saccharomyces Cerevisiae, then add pyridine, and stirring is to dissolving, and at 60～70 DEG C of water-baths 2～3h, reaction terminates
After be cooled to room temperature, reactant liquor is added distilled water, with 2.5mol/L NaOH regulation pH value to 7～8, centrifugal abandons insoluble matter, add
95% ethanol of 4～5 times of volumes, separates out precipitation, precipitation is dissolved in water, with distilled water dialysis 36～48h, concentrates, and lyophilization is i.e.
?.
Compared with prior art, it provides the benefit that the present invention: utilize polysaccharide and oligosaccharide that waste beer yeast mud extracts, and
Modifying polysaccharide, add the biological activity of zymosan, after sulphation, zymosan is to animal immunizing power and antiviral
Ability improves notable, and meanwhile, the total bacteria count of the clostridium butyricum culture fluid screened is up to 1.9 × 109, three kinds prebiotic
Bacterium combines, and the most effectively suppresses the growth and breeding of pathogenic microorganism, improves efficiency of feed utilization, promotes growth of animal, carries
High animal resistance, resistance and the immunity of enhancing body have remarkable effect, can effectively suppress pathogen in animal body.Raw
Production. art is stable, technology maturation, is suitable for large-scale production and promotes.
Detailed description of the invention
Embodiment 1
A kind of efficiently Synbiotic feed additive, its composition mass percent is: 45～the probiotic bacteria of 50% and 50～the benefit of 55%
Raw unit, wherein probiotic bacteria composition mass percent is: clostridium butyricum 30～40%, lactic acid bacillus 45～55%,
Bafillus natt 10～20%；Described prebiotics composition mass percent is: Analysis of Polysaccharide From Saccharomyces Cerevisiae 60～70%, sulphation medicated beer
Zymosan 30～40%.
The total plate count 1.6 × 10 of clostridium butyricum in described probiotic bacteria9～1.9 × 109Cfu/g, lactic acid bud
The total plate count 6 × 10 of spore bacillus8～9 × 108Cfu/g, the total plate count 1 × 10 of Bafillus natt9～2 × 109 cfu/
g。
The preparation method of described clostridium butyricum is: takes strain and is inoculated in clostridium butyricum culture medium
In, 36～38 DEG C of constant temperature stand, and Anaerobic culturel 20～24h obtains first order seed bacterium, first order seed bacterium is inoculated into butanoic acid shuttle shape bud
In spore baccilus medium, obtaining secondary seed bacterium solution, wherein first order seed bacterium with clostridium butyricum culture medium mass ratio is
1:15～24；Take clostridium butyricum culture medium dilute 19～21 times, in 110～120 DEG C of steam sterilizations 20～
30min, is cooled to 35～40 DEG C, adds secondary seed bacterium solution, stirs, constant temperature 35～45 DEG C of fermentation culture 32～40h, institute
The volume ratio of the clostridium butyricum kind culture medium after adding secondary seed bacterium solution and diluting is 1:7～12；After having fermented,
Take fermentation liquid and be cooled to room temperature, then 2500～3000r/min be centrifuged 15～20min, remove supernatant, with the Lac Bovis seu Bubali of 20-30%
Bacterium mud is washed out by juice as protective agent, then it is mixed with the liquid that is coated containing gelatin, and homogenizing is coated, lyophilization, grinds
Become powder.
The composition weight portion of described clostridium butyricum culture medium is: yeast extract 5, peptone 10, glucose 10, cattle
Meat extractum 3, ammonium sulfate 1, NaCl 2, dipotassium hydrogen phosphate 4, sodium bicarbonate 1, MnSO4 0.2、 MgSO4 7H2O 0.5、
CaCO31, distilled water 1000.
The preparation method of described Analysis of Polysaccharide From Saccharomyces Cerevisiae is: take beer yeast slurry, cleans with distilled water, vacuum filtration, then
Again wash, after placing 0.5～1 h, outwell supernatant, centrifugal, add 0.5% sodium bicarbonate of twice beer yeast slurry volume
Washing 1～2 h, then wash twice, centrifugal, abandon supernatant and obtain clean beer yeast, be converted to dry ferment according to its water content
Female weight, adds appropriate water and Sal makes yeast and brine concentration be 8～10%, be placed in high pressure homogenizer by solution,
Temperature 110～120 DEG C, low pressure 15MPa, each homogenizing 3 times under conditions of high pressure 65MPa, cooling, high speed centrifugation, retain supernatant,
Being concentrated into the 1/4～1/5 of original volume, make precipitant with 95% ethanol, precipitate 10～12h, ethanol is 2 with the volume ratio of supernatant
～3:1, precipitation is used washing with acetone 2 times, is washed 1 time with ether, finally will be deposited at 30～40 DEG C and be dried 12～16h, and to obtain final product.
The preparation method of described sulphation Analysis of Polysaccharide From Saccharomyces Cerevisiae is: in mass ratio 2.0～4.0:1 weigh sulfur trioxide and beer
Brewer yeast polysaccharide, then adds pyridine, and stirring is to dissolving, and at 60～70 DEG C of water-baths 2～3h, reaction is cooled to room after terminating
Temperature, adds distilled water by reactant liquor, with 2.5mol/L NaOH regulation pH value to 7～8, is centrifuged and abandons insoluble matter, add 4～5 times of volumes
95% ethanol, separate out precipitation, precipitation be dissolved in water, dialyse 36～48h with distilled water, concentrate, lyophilization and get final product.
Embodiment 2
A kind of efficiently Synbiotic feed additive, its composition mass percent is: the probiotic bacteria of 45% and the prebiotics of 55%, wherein
Probiotic bacteria composition mass percent is: clostridium butyricum 30%, lactic acid bacillus 50%, Bafillus natt 20%；Institute
Stating prebiotics composition mass percent is: Analysis of Polysaccharide From Saccharomyces Cerevisiae 60%, sulphation Analysis of Polysaccharide From Saccharomyces Cerevisiae 40%.
The total plate count 1.6 × 10 of clostridium butyricum in described probiotic bacteria9Cfu/g, the bacterium of lactic acid bacillus
Fall sum 6 × 108Cfu/g, the total plate count 2 × 10 of Bafillus natt9 cfu/g。
Embodiment 3
A kind of efficiently Synbiotic feed additive, its composition mass percent is: the probiotic bacteria of 50% and the prebiotics of 50%, wherein
Probiotic bacteria composition mass percent is: clostridium butyricum 40%, lactic acid bacillus 50%, Bafillus natt 10%；Institute
Stating prebiotics composition mass percent is: Analysis of Polysaccharide From Saccharomyces Cerevisiae 70%, sulphation Analysis of Polysaccharide From Saccharomyces Cerevisiae 30%.
The total plate count 1.9 × 10 of clostridium butyricum in described probiotic bacteria9Cfu/g, the bacterium of lactic acid bacillus
Fall sum 6 × 108Cfu/g, the total plate count 1 × 10 of Bafillus natt9cfu/g。
Embodiment 4
A kind of efficiently Synbiotic feed additive, its composition mass percent is: the probiotic bacteria of 48% and the prebiotics of 52%, wherein
Probiotic bacteria composition mass percent is: clostridium butyricum 35%, lactic acid bacillus 55%, Bafillus natt 10%；Institute
Stating prebiotics composition mass percent is: Analysis of Polysaccharide From Saccharomyces Cerevisiae 65%, sulphation Analysis of Polysaccharide From Saccharomyces Cerevisiae 35%.
The total plate count 1.9 × 10 of clostridium butyricum in described probiotic bacteria9Cfu/g, the bacterium of lactic acid bacillus
Fall sum 8 × 108Cfu/g, the total plate count 1 × 10 of Bafillus natt9 cfu/g。
The present embodiment is most preferred embodiment, and existing for the present embodiment products substitution antibiotic feed is cultivated growing and fattening pigs, expects meat
Ratio reaches 1.7:1, and daily gain reaches 730-900g, delivers natural law for sale and shifts to an earlier date 10 days, and dressing percentage is more than 70%, improves live pig simultaneously
Immunity and premunition, economic benefit is notable with social benefit.
The present invention utilizes the polysaccharide and oligosaccharide that waste beer yeast mud extracts, and modifies polysaccharide, adds yeast many
The biological activity of sugar, after sulphation, animal immunizing power and anti-virus ability are improved notable by zymosan, meanwhile, the fourth screened
The total bacteria count of acid clostridium culture fluid is up to 1.9 × 109, three kinds of probiotic combinations, the most effectively suppress disease
The growth and breeding of pathogenic microorganism, improves efficiency of feed utilization, promotes growth of animal, improves animal resistance, the opposing of enhancing body
Power and immunity have remarkable effect, can effectively suppress pathogen in animal body.Stable processing technique, technology maturation, it is suitable for big
Large-scale production is promoted.
Claims (6)
1. an efficient Synbiotic feed additive, it is characterised in that composition mass percent be: 45～50% probiotic bacteria and
50～the prebiotics of 55%, wherein probiotic bacteria composition mass percent is: clostridium butyricum 30～40%, lactic acid spore bar
Bacterium 45～55%, Bafillus natt 10～20%；Described prebiotics composition mass percent is: Analysis of Polysaccharide From Saccharomyces Cerevisiae 60～70%,
Sulphation Analysis of Polysaccharide From Saccharomyces Cerevisiae 30～40%.
Efficient Synbiotic feed additive the most according to claim 1, it is characterised in that: butanoic acid shuttle shape in described probiotic bacteria
The total plate count 1.6 × 10 of bacillus cereus9～1.9 × 109Cfu/g, the total plate count 6 × 10 of lactic acid bacillus8～9 ×
108Cfu/g, the total plate count 1 × 10 of Bafillus natt9～2 × 109 cfu/g。
Efficient Synbiotic feed additive the most according to claim 1, it is characterised in that: described clostridium butyricum
Preparation method be: taking strain and be inoculated in clostridium butyricum culture medium, 36～38 DEG C of constant temperature stand, Anaerobic culturel 20
～24h, obtain first order seed bacterium, first order seed bacterium be inoculated in clostridium butyricum culture medium, obtain secondary seed bacterium solution,
Wherein first order seed bacterium and clostridium butyricum culture medium mass ratio are 1:15～24；Take clostridium butyricum to cultivate
Base dilute 19～21 times, in 110～120 DEG C of steam sterilizations 20～30min, be cooled to 35～40 DEG C, adds secondary seed
Bacterium solution, stirs, constant temperature 35～45 DEG C of fermentation culture 32～40h, the butanoic acid shuttle shape after added secondary seed bacterium solution and dilution
The volume ratio of bacillus cereus kind culture medium is 1:7～12；After having fermented, take fermentation liquid and be cooled to room temperature, then 2500～
3000r/min is centrifuged 15～20min, removes supernatant, is washed out by bacterium mud as protective agent with the Lac Bovis seu Bubali juice of 20-30%, then will
It mixes with the liquid that is coated containing gelatin, and homogenizing is coated, lyophilization, grind into powder.
Efficient Synbiotic feed additive the most according to claim 3, it is characterised in that: described clostridium butyricum
The composition weight portion of culture medium is: yeast extract 5, peptone 10, glucose 10, beef extract 3, ammonium sulfate 1, NaCl 2, phosphoric acid
Hydrogen dipotassium 4, sodium bicarbonate 1, MnSO4 0.2、 MgSO4 7H2O 0.5、 CaCO31, distilled water 1000.
Pig green feed the most according to claim 1, it is characterised in that: the preparation method of described Analysis of Polysaccharide From Saccharomyces Cerevisiae
It is: take beer yeast slurry to clean with distilled water, vacuum filtration, the most again wash, after placing 0.5～1 h, outwells supernatant,
Centrifugal, add 0.5% sodium bicarbonate washing 1～2 h of twice beer yeast slurry volume, then wash twice, centrifugal, abandon supernatant
Obtain clean beer yeast, be converted to dry yeast weight according to its water content, add appropriate water and Sal makes yeast and food
Salinity is 8～10%, is placed in high pressure homogenizer by solution, at temperature 110～120 DEG C, low pressure 15MPa, high pressure 65MPa
Under conditions of each homogenizing 3 times, cooling, high speed centrifugation, retain supernatant, be concentrated into the 1/4～1/5 of original volume, make with 95% ethanol
Precipitant, precipitates 10～12h, and ethanol is 2～3:1 with the volume ratio of supernatant, and precipitation uses washing with acetone 2 times, washs 1 with ether
Secondary, finally will be deposited at 30～40 DEG C and be dried 12～16h, to obtain final product.
Efficient Synbiotic feed additive the most according to claim 1, it is characterised in that: described sulphation beer yeast is many
The preparation method of sugar is: in mass ratio 2.0～4.0:1 weigh sulfur trioxide and Analysis of Polysaccharide From Saccharomyces Cerevisiae, then add pyridine, stirring
To dissolving, at 60～70 DEG C of water-baths 2～3h, reaction is cooled to room temperature after terminating, reactant liquor adds distilled water, uses
2.5mol/L NaOH regulation pH value is to 7～8, and centrifugal insoluble matter of abandoning, 95% ethanol of 4～5 times of volumes of addition, precipitation precipitates, will
Precipitation is dissolved in water, dialyses 36～48h with distilled water, concentrates, lyophilization and get final product.
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Cited By (1)
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Application publication date: 20161109 |