CN106069764B - A kind of ornamental plant culture medium, bottled ornamental plant and preparation method thereof - Google Patents
A kind of ornamental plant culture medium, bottled ornamental plant and preparation method thereof Download PDFInfo
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- CN106069764B CN106069764B CN201610487136.9A CN201610487136A CN106069764B CN 106069764 B CN106069764 B CN 106069764B CN 201610487136 A CN201610487136 A CN 201610487136A CN 106069764 B CN106069764 B CN 106069764B
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- plant
- culture medium
- ornamental plant
- ornamental
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention is suitable for technical field of plant culture, there is provided a kind of ornamental plant culture medium, bottled ornamental plant and preparation method thereof, the formula of this ornamental plant culture medium are：1/2MS culture mediums are basic raw material, while add 1.8 2.3g/L of agar；7 9g/L of plant gel；48 53mg/L of diaminopimelic acid；PQQ9.5‑10.5umol/L；23 26mg/L of kanamycins, the pH value for then adjusting ornamental plant culture medium are 6.5 6.9.Culture medium provided by the invention can accelerate plant strain growth, and also comparatively robust, suitable plant grow culture medium for a long time, and be especially suitable for the culture medium in bottled ornamental plant, plant strain growth is more vigorous, and stability is stronger, and ornamental value is more preferable, and growth cycle is longer.
Description
Technical field
The invention belongs to technical field of plant culture, more particularly to a kind of ornamental plant culture medium, bottled ornamental plant and
Its production method.
Background technology
Bottled ornamental plant is a kind of plantlet being mounted in miniature bottle, can be carried with, without watering, fertilizing, in bottle
Culture medium provide necessary nutrient for plantlet growth, life cycle is also relatively more long, has important enjoying value,
Playability is also very high.
Current ornamental plant culture medium prescription of viewing and admiring generally uses standard MS medium, its main feature is that inorganic salts and ion
Concentration is higher, is more stable ionic equilibrium solution, its nitrate content is high, and the quantity and ratio of its nutrient are suitable, can be full
The nutrition and physiological requirements of sufficient plant cell, thus the scope of application is wider, most plants tissue-culturing quick-propagation is made with it
For the minimal medium of culture medium.
But since MS culture mediums solidness is insufficient, after the first segment time, the easy run-off the straight of plantlet in bottle,
Even all sink in culture medium.Also coagulator is added in some culture mediums having, these coagulators are unfavorable for plant growth, raw
It is too short to order the cycle.
The content of the invention
In view of the above problems, it is an object of the invention to provide a kind of ornamental plant culture medium, bottled ornamental plant and its
Production method, it is intended to solve existing ornamental plant culture medium solidness not enough or be unfavorable for the technical problem of plant growth.
The present invention adopts the following technical scheme that：
On the one hand, the ornamental plant culture medium is using 1/2MS culture mediums as basic raw material, at the same add agar, plant coagulates
Glue, diaminopimelic acid, pyrroloquinoline quinone PQQ and kanamycins so that in obtained ornamental plant culture medium：
The pH value of ornamental plant culture medium is 6.5-6.9.
On the other hand, the bottled production of ornamental plant, comprises the following steps：
Explant is cultivated, the explant sterilization treatment that carries out disinfection is obtained into aseptic seedling；
The aseptic seedling is inoculated on standard MS medium culture medium, is placed in suitable illumination, temperature, the culturing room of humidity
Culture 25-35 days, obtains plant shoots；
Suitable size, height, well-grown aseptic plant seedling inoculation are selected in the environment of sterile working to being equipped with
In the micro bottles of ornamental plant culture medium；
The third aspect, the bottled ornamental plant include micro bottles, ornamental plant culture medium, institute are marked with the micro bottles
State in ornamental plant culture medium and be implanted with plant shoots.
The beneficial effects of the invention are as follows：Based on the present invention uses 1/2MS culture mediums, plant is set to promote into starvation
Take root, and add other helper components, so culture medium can be made more to harden but not hinder plant growth, and plant growth
It is more vigorous, growth cycle is longer.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, by the following examples to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Then ornamental plant culture medium provided in this embodiment adds agar, plant using 1/2MS culture mediums as basic raw material
Gel, diaminopimelic acid, PQQ and kanamycins, finally so that agar 1.8-2.3g/L；Plant gel 7-9g/L；Diamino
Base pimelic acid 48-53mg/L；PQQ 9.5-10.5umol/L；Kanamycins 23-26mg/L.Then ornamental plant culture medium is adjusted
PH value be 6.5-6.9.
It is most of that the component of standard MS medium is broadly divided into six：A great number of elements, trace element, glycine, inositol, dimension life
Element and molysite.And compared with standard MS medium, a great number of elements halves 1/2MS culture mediums here, other compositions are constant.1/2MS
Culture medium can also directly be bought, commercially available, by using 1/2MS culture mediums, plant is entered starvation and promoted life
Root, root is more long more in the medium, and plant is more firm.The agar and plant gel can improve the firm of culture medium
Degree, after plant establishment, can collapse to avoid plant.The diaminopimelic acid, kanamycins and micro PQQ, Neng Gouqi
To the effect for promoting plant growth.
It is specific to cultivate explant first when making, the explant sterilization treatment that carries out disinfection is obtained into aseptic seedling；By the nothing
Bacterination obtained plant shoots by 25-35 days on standard MS medium；Selected in the environment of sterile working suitable size,
Highly, well-grown aseptic plant seedling inoculation is into the micro bottles equipped with ornamental plant culture medium.
The effect of this ornamental plant culture medium is verified below by several test examples and comparative example.
First, ornamental plant culture medium, wherein agar 2g/L are configured；Plant gel 8g/L；Diaminopimelic acid 50mg/L；
PQQ 10umol/L；Kanamycins 25mg/L, the pH value for adjusting ornamental plant culture medium are 6.7.Above-mentioned culture medium is divided into 5
Part, it is respectively labeled as 1-5 culture mediums.Configuration standard MS culture mediums, labeled as No. 6 culture mediums；Configuration standard MS culture mediums are simultaneously
Add solid coagulator agar, content 8g/L, labeled as No. 7 culture mediums.The weight such as 1-7 culture mediums.
Then, jonquil explant is cultivated, explant is carried out disinfection sterilization treatment, obtains aseptic seedling；Then will be sterile
Seedling is inoculated on standard MS medium culture medium, is placed in suitable illumination, culturing room's growth one month or so of temperature, humidity, is obtained
To plant shoots, wherein excessive or too small plant shoots are removed so that the plant shoots size in standard MS medium is basic
It is identical.
In the environment of sterile working, in 1-7 culture mediums, every part of culture medium is inoculated with 20 plants of plant shoots at random, so
A period of time is cultivated in same environment (suitable illumination, temperature, humidity) culturing room afterwards, obtains jonquil plant.
Jonquil plant strain growth situation in each culture medium is counted, as a result such as following table：
As can be known from the above table, the jonquil that 1-5 medium cultures the obtain plant height that is averaged is 20.8mm, and average strain is wide
24.2mm, maximum root long average length are 32.4mm.Compared to No. 6 culture mediums using standard MS medium and addition solidification
No. 7 culture mediums of agent, the jonquil cultivated using the 1-5 culture mediums of the present embodiment culture medium no matter from plant height and
For plant width and root growth situation, all it is significantly improved, it is ensured that the quick healthy growth of plant.And No. 6 trainings
Growing up with plant in base is supported, substantial amounts of plant tilt phenomenon occurs, if continuing to cultivate, No. 6 culture mediums may can not
Load-bearing, plant can more tilt, or even sink in culture medium, although and do not occur plant tilt phenomenon in No. 7 culture mediums,
The speed of growth of plant still lags far behind 1-5 culture mediums.
Therefore culture medium provided by the invention can accelerate plant strain growth, and culture medium also comparatively robust, be adapted to plant
Grow for a long time, be especially suitable for the culture medium in bottled ornamental plant.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (3)
1. a kind of ornamental plant culture medium, it is characterised in that the ornamental plant culture medium is former based on 1/2MS culture mediums
Material, while add agar, plant gel, diaminopimelic acid, pyrroloquinoline quinone PQQ and kanamycins so that obtained sight
Appreciate in plant culture：
Agar 1.8-2.3g/L；
Plant gel 7-9g/L；
Diaminopimelic acid 48-53mg/L；
PQQ 9.5-10.5 umol/L；
Kanamycins 23-26mg/L.
2. ornamental plant culture medium as claimed in claim 1, it is characterised in that in the ornamental plant culture medium：
Agar 2g/L；
Plant gel 8g/L；
Diaminopimelic acid 50mg/L；
PQQ 10 umol/L；
Kanamycins 25mg/L.
3. ornamental plant culture medium as claimed in claim 1 or 2, it is characterised in that the ornamental plant culture medium adjusts pH value
For 6.5-6.9.
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| CN201610487136.9A CN106069764B (en) | 2016-06-28 | 2016-06-28 | A kind of ornamental plant culture medium, bottled ornamental plant and preparation method thereof |
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| CN108849515B (en) * | 2018-07-24 | 2019-11-29 | 湖南美可达生物资源股份有限公司 | A kind of culture medium and method of the generation of induction macleaya cordata embryo shape callus |
| CN109197584A (en) * | 2018-08-03 | 2019-01-15 | 南京龙源生态农业有限公司 | A kind of dendrobium candidum root media and its application |
| CN109197585A (en) * | 2018-08-03 | 2019-01-15 | 南京龙源生态农业有限公司 | A kind of method of dendrobium candidum sprouting and rooting |
| CN109197586A (en) * | 2018-08-03 | 2019-01-15 | 南京龙源生态农业有限公司 | A kind of dendrobium candidum Protocorm base and its application |
| CN109042338A (en) * | 2018-10-17 | 2018-12-21 | 徐颂涛 | One kind preventing sky seedling watermelon seedling cultural method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1806528A (en) * | 2006-02-13 | 2006-07-26 | 陈曦 | Sterile culture method living plant artwork |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1806528A (en) * | 2006-02-13 | 2006-07-26 | 陈曦 | Sterile culture method living plant artwork |
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Effective date of registration: 20170724 Address after: 430000 Hubei Dongxin Wuhan Development Zone East Lake Road, SBI Venture Street 1 Building 2 unit 1309 Applicant after: Wuhan high Yuanxin Technology Co. Ltd. Address before: 430000 Hubei Province, Wuhan City Qingshan District Flower Village 119 neighborhood 75 Gate No. 8 Applicant before: Wuhan Bo Kang source Biotechnology Co. Ltd. |
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Inventor after: Zhang Bocong Inventor after: Qi Yingchun Inventor after: Zhou Shangwen Inventor after: Yuan Ruichen Inventor after: Wang Xueqiong Inventor before: Zhang Bocong Inventor before: Qi Yingchun |
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