CN106068273B - 增强的蛋白质表达 - Google Patents

增强的蛋白质表达 Download PDF

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Publication number
CN106068273B
CN106068273B CN201480076332.6A CN201480076332A CN106068273B CN 106068273 B CN106068273 B CN 106068273B CN 201480076332 A CN201480076332 A CN 201480076332A CN 106068273 B CN106068273 B CN 106068273B
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protein
amino acid
bacillus
seq
interest
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Chinese (zh)
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CN106068273A (zh
Inventor
C·邦焦尔尼
B·F·施密特
R·奥楚卡
A·范齐蒙耐德
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Danisco US Inc
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Danisco US Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN201480076332.6A 2013-12-31 2014-12-08 增强的蛋白质表达 Active CN106068273B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361922613P 2013-12-31 2013-12-31
US61/922,613 2013-12-31
PCT/US2014/069019 WO2015102814A1 (en) 2013-12-31 2014-12-08 Enhanced protein expression

Publications (2)

Publication Number Publication Date
CN106068273A CN106068273A (zh) 2016-11-02
CN106068273B true CN106068273B (zh) 2021-01-08

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CN201480076332.6A Active CN106068273B (zh) 2013-12-31 2014-12-08 增强的蛋白质表达

Country Status (8)

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US (1) US10287591B2 (cg-RX-API-DMAC10.html)
EP (1) EP3089991B1 (cg-RX-API-DMAC10.html)
JP (1) JP6585602B2 (cg-RX-API-DMAC10.html)
CN (1) CN106068273B (cg-RX-API-DMAC10.html)
CA (1) CA2935066C (cg-RX-API-DMAC10.html)
DK (1) DK3089991T3 (cg-RX-API-DMAC10.html)
MX (1) MX2016008595A (cg-RX-API-DMAC10.html)
WO (1) WO2015102814A1 (cg-RX-API-DMAC10.html)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106599611B (zh) * 2016-12-09 2019-04-30 中南大学 蛋白质功能标注方法及系统

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008066931A8 (en) * 2006-11-29 2009-07-16 Novozymes Inc Bacillus licheniformis chromosome

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US5763257A (en) 1984-05-29 1998-06-09 Genencor International, Inc. Modified subtilisins having amino acid alterations
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
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US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
EP0254735B2 (en) 1986-01-15 1998-06-17 Amgen Inc. METHOD FOR PRODUCTION OF THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS
US4980288A (en) 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
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PT86864B (pt) 1987-02-27 1992-05-29 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
US4914031A (en) 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
PT89702B (pt) 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
US5665587A (en) 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK97190D0 (da) 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
US5482849A (en) 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
KR100258460B1 (ko) 1991-05-01 2000-06-01 한센 핀 베네드 안정화 효소 및 세제 조성물
DE4411223A1 (de) 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US6835550B1 (en) 1998-04-15 2004-12-28 Genencor International, Inc. Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins
US6617148B2 (en) * 2000-06-29 2003-09-09 E. I. Du Pont De Nemours And Company Natural promoters for gene expression and metabolic monitoring in bacillus species
ATE495245T1 (de) 2002-02-15 2011-01-15 Danisco Us Inc Verbesserte proteinexpression in bacillus subtilis
BRPI0517908A (pt) * 2004-11-26 2008-10-14 Kyowa Hakko Kogyo Kk cepa mutante de e. coli, e, método para produzir uma substáncia útil
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Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008066931A8 (en) * 2006-11-29 2009-07-16 Novozymes Inc Bacillus licheniformis chromosome

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A peptidoglycan fragment triggers[beta]-lactam resistance in bacillus licheniformis;ANA AMOROSO ET AL;《PLOS PATHOGENS》;20120315;第6页左栏最后一段和右栏第一段，和SUPPLEMENTAL DATA *
A vector for systematic gene inactivation in Bacillus subtilis;Dervyn E et.al;《Microbiology》;19981130;3097-3104 *
ANA AMOROSO ET AL.A peptidoglycan fragment triggers[beta]-lactam resistance in bacillus licheniformis.《PLOS PATHOGENS》.2012,e1002571. *

Also Published As

Publication number Publication date
CA2935066A1 (en) 2015-07-09
EP3089991A1 (en) 2016-11-09
EP3089991B1 (en) 2019-08-28
JP2017500879A (ja) 2017-01-12
MX2016008595A (es) 2016-10-28
JP6585602B2 (ja) 2019-10-02
DK3089991T3 (da) 2019-12-02
CN106068273A (zh) 2016-11-02
US20160319288A1 (en) 2016-11-03
WO2015102814A1 (en) 2015-07-09
CA2935066C (en) 2022-06-21
US10287591B2 (en) 2019-05-14

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