CN106053702A - Multi-ingredient content measuring method of Jiaweixiaoyao pills - Google Patents
Multi-ingredient content measuring method of Jiaweixiaoyao pills Download PDFInfo
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- G—PHYSICS
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention belongs to the technical field of traditional Chinese medicine preparation quality control, and particularly relates to a content measuring method of Jiaweixiaoyao pills. The method has the technical scheme that the multi-ingredient content measuring method of the Jiaweixiaoyao pills comprises the following steps of A, preparing a reference substance solution; B, preparing a test article solution; C, performing detection by a high performance liquid chromatograph instrument (comprising a DAD detector) under the chromatograph conditions that the chromatograph column is Plastisil ODS C18, the acetonitrile-phosphoric acid water is used as a flowing phase, the flowing speed is 0.8mL/min to 1.2mL/min, the column temperature is 35 to 39 DEG C, the detection wave length is 200nm to 400nm, and the sample feeding quantity is 5 to 20 muL; D, calculating the content of each ingredient. The method has the advantages that the contents of five ingredients including geniposide, liquiritin, paeonol, paeoniflorin, glycyrrhizic acid and ferulic acid in the Jiaweixiaoyao pills are measured at the same time; the operation is simple; the sensitivity is high; the reproducibility is high; the detection result is accurate and reliable.
Description
Technical field
The invention belongs to Chinese medicine preparation Quality Control Technology field, be specifically related to a kind of JIAWEI XIAOYAOWAN assay side
Method.
Background technology
JIAWEI XIAOYAOWAN is mainly by Radix Bupleuri, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Fructus Gardeniae, the Rhizoma Atractylodis Macrocephalae, Poria, Radix Glycyrrhizae, Cortex Moutan, nine kinds of medicines of Herba Menthae
Material forms, and for Traditional Chinese medicine historical preparation, has effect of Shugan Qingre, strengthening spleen and nourishing blood, is mainly used in hepatic depression and blood deficiency, liver spleen clinically
Discord, two side of body distending pains, have a dizzy spell, asthenia lack of appetite, menoxenia, umbilicus abdominal distention and pain etc..Wherein ferulic acid is Radix Angelicae Sinensis primary chemical
Composition；Peoniflorin is the main chemical compositions of the Radix Paeoniae Alba；Glycyrrhizic acid, liquirtin are the main chemical compositions of Radix Glycyrrhizae；Paeonol is male
The principal character composition of Cortex Moutan；Jasminoidin is the main chemical compositions of Fructus Gardeniae.
At present about only a kind of with peoniflorin in the assay item in the quality standard of JIAWEI XIAOYAOWAN in Chinese Pharmacopoeia
The content of composition controls the quality of this medicine, it is difficult to the real inherent quality embodied and ensure JIAWEI XIAOYAOWAN.
In existing document, the most on the books: jasminoidin and Radix Paeoniae in hplc simultaneous determination JIAWEI XIAOYAOWAN
The content of glycosides；The content of paeonol in high effective liquid chromatography for measuring JIAWEI XIAOYAOWAN；It is free and unfettered that high effective liquid chromatography for measuring adds taste
Peoniflorin and the content of liquirtin in distant ball.
Obviously cannot thoroughly evaluating and control JIAWEI XIAOYAOWAN quality by 1-2 kind chemical composition.But can simply, accurately survey
Determine many component contents of JIAWEI XIAOYAOWAN or a technical barrier.
Summary of the invention
It is an object of the invention to: in order to overcome deficiency of the prior art, the present invention provides a kind of JIAWEI XIAOYAOWAN many
Component content assay method, measures jasminoidin, liquirtin, paeonol, peoniflorin, glycyrrhizic acid, Resina Ferulae in JIAWEI XIAOYAOWAN simultaneously
The content of 6 kinds of main components of acid.
The technical scheme is that the multicomponent content assaying method of a kind of JIAWEI XIAOYAOWAN, it is characterized in that perform with
Lower step:
A. reference substance solution is prepared: weigh a certain amount of jasminoidin respectively, liquirtin, paeonol, peoniflorin, glycyrrhizic acid,
Ferulic acid reference substance, is dissolved with solvent, obtains certain density reference substance storing solution；Precision amount is a certain amount of described right respectively
Add absolute methanol dilution according to product storing solution, obtain reference substance solution；
B. prepare need testing solution: JIAWEI XIAOYAOWAN to be measured be dissolved in solvent, filter, obtain certain density for examination
Product solution；
C. detecting with high performance liquid chromatograph, wherein said high performance liquid chromatograph includes DAD detector, chromatostrip
Part: chromatographic column is Plastisil ODS C18, acetonitrile phosphoric acid water for flowing phase, flow velocity be 0.8mL/min～1.2mL/min,
Column temperature is 35 DEG C～39 DEG C, detection wavelength is 200nm～400nm, sample size 5-20 μ L；
D. the content of 6 kinds of compositions in described need testing solution is calculated: calculate described in described need testing solution with external standard method
Jasminoidin, liquirtin, paeonol, peoniflorin, glycyrrhizic acid, the content of 6 kinds of compositions of ferulic acid.
Further, described solvent is absolute methanol.
Further, described detector is diode array detector, and DAD reference wavelength is detection wavelength described in 380nm
For 230nm, 238nm, 254nm, 274nm, 328nm, corresponding composition to be measured is: 230nm is peoniflorin, 238nm be jasminoidin,
254nm is glycyrrhizic acid, and 274nm is paeonol and liquirtin, and 328nm is ferulic acid.
Further, described flowing is acetonitrile 0.1～0.2% phosphoric acid water mutually；
Further, described flowing is acetonitrile 0.2% phosphoric acid water mutually；Described flow velocity is 1.0mL/min；Described column temperature 37
℃；Described sample size 10 μ L
Further, gradient elution when described high performance liquid chromatograph detects, gradient elution order:
0～10min, acetonitrile concentration is increased to 8% by 5%；
10～15min, acetonitrile concentration is increased to 10% by 8%；
15～20min, acetonitrile concentration is increased to 13% by 10%；
20～25min, acetonitrile concentration is increased to 16% by 13%；
25～28min, acetonitrile concentration is increased to 20% by 16%；
28～36min, acetonitrile concentration is increased to 22% by 20；
36～40min, acetonitrile concentration is 22%；
40～43min, acetonitrile concentration is increased to 27% by 22%；
43～51min, acetonitrile concentration is increased to 40% by 27%；
51～60min, acetonitrile concentration is increased to 50% by 40%；
60～70min, acetonitrile concentration is increased to 75% by 50%；
70～75min, acetonitrile concentration is increased to 90% by 75%；
75～80min, acetonitrile concentration is 90%.
Further, the preparation method of need testing solution described in step B is:
Take 0.5～1.5g JIAWEI XIAOYAOWAN to be measured, accurately weighed, the accurate absolute methanol 25～75mL that adds, weighed weight,
Supersound process 20～40min, lets cool, and supplies the weight of less loss with absolute methanol, shakes up, centrifugal, takes supernatant, through 0.22～
0.45 μm microporous filter membrane filters, and obtains described need testing solution.
Further, the preparation method of need testing solution described in step B is:
Weighed the JIAWEI XIAOYAOWAN powder about 1.0g to be measured of 40 mesh sieves, accurately weighed, put 100mL ground tool plug conical flask
In, the accurate absolute methanol 50mL that adds, close plug, weighed weight, with power 200W, frequency 80kHz supersound process 30min, cool down,
The most weighed weight, supplies the weight of less loss, shakes up with absolute methanol, is centrifuged with 12000r/min rotating speed, takes supernatant, warp
0.45 μm microporous filter membrane filters, and obtains described need testing solution.
Further, the preparation method of reference substance solution described in step A is:
Weigh jasminoidin, liquirtin, paeonol, peoniflorin, glycyrrhizic acid, ferulic acid reference substance respectively in right amount, add without water beetle
Alcohol dissolves and constant volume, makes mass concentration and is respectively jasminoidin 0.6 mg/mL, liquirtin 0.75mg/mL, paeonol 0.8mg/
ML, peoniflorin 1.2mg/mL, glycyrrhizic acid 0.8mg/mL, the mixing reference substance storing solution of ferulic acid 0.25mg/mL, precision is inhaled respectively
Take 2,4,6,8,12mL storing solution be placed in 25mL volumetric flask, flow phase constant volume, prepares the mixing reference substance solution of series concentration.
Beneficial effects of the present invention mainly has: the present invention uses high performance liquid chromatography (HPLC) under same chromatographic condition
Measure jasminoidin in JIAWEI XIAOYAOWAN, liquirtin, paeonol, peoniflorin, glycyrrhizic acid, the content of 6 kinds of compositions of ferulic acid simultaneously, adopt
With gradient elution, in 80min, complete a chromatography, mean sample recovery rate 99.13%～100.25%, RSD value all <
2.0%.The method is simple to operate, highly sensitive, favorable reproducibility, testing result accurately and reliably, the quality for JIAWEI XIAOYAOWAN is commented
Valency provides a kind of effective detection method, can be used for the quality control of JIAWEI XIAOYAOWAN, quality evaluation, for quality evaluation
Raising with quality standard provides technical support.
Accompanying drawing explanation
Fig. 1 respectively detects the ultraviolet spectrogram of each composition to be measured under wavelength: A-jasminoidin uv-spectrogram；B-liquirtin ultraviolet figure
Spectrum；C-paeonol uv-spectrogram；D-peoniflorin uv-spectrogram；E-glycyrrhizic acid uv-spectrogram；F-ferulic acid uv-spectrogram.
Detailed description of the invention
In order to more intuitively more clearly describe technical solution of the present invention, and help to understand the present invention tribute to prior art
Offer part, below in conjunction with drawings and Examples, the present invention is described in detail.
Embodiment 1
The present embodiment is the multicomponent content assaying method of a kind of JIAWEI XIAOYAOWAN, and selected instrument is as follows with material:
1260 type high performance liquid chromatographs (include DAD detector, quarternary low pressure gradient pump, online vacuum degassing machine),
ChemStation work station (Agilent company of the U.S.), AUW220D type analysis balance (100,000/, Japan's Shimadzu is accurate
Instrumental Analysis company), KQ-250DE type ultrasonic cleaner (Fauna of Kunshan, Jiangsu ultrasonic instrument company limited)
Jasminoidin (lot number: MUST-15022411, purity 98.13%)；Ferulic acid (lot number: MUST-14102214, purity
99.36%)；Liquirtin (lot number: MUST-14100910, purity 99.83%)；Peoniflorin (lot number: MUST-15060112, pure
Degree 99.42%)；Glycyrrhizic acid (lot number: MUST-14091708, purity 98.69%)；Paeonol (lot number: MUST-15020401,
Purity 99.30%)；Above-mentioned reference substance is all purchased from Man Site bio tech ltd, Chengdu；Acetonitrile (lot number: 20150833, color
Compose pure), methanol (lot number: 20150322, chromatographically pure) provides by Shandong YuWang Industry Co., Ltd；Water is that commercially available WAHAHA is pure
Water, other reagent is analytical pure；
JIAWEI XIAOYAOWAN (lot number: 20150644,20150811,20150810, specification: 6g/ bag) by Beijing Tongrentang system
Medicine limited company provides；JIAWEI XIAOYAOWAN (lot number: 1106208,1105122,1105803, specification: 6g/ bag) by Tianjin
It scholar's power (Liaoning) Pharmaceutical Co provides.
Concrete below the step that performs:
A. reference substance solution is prepared: weigh jasminoidin, liquirtin, paeonol, peoniflorin, glycyrrhizic acid, ferulic acid pair respectively
Appropriate according to product, add absolute methanol and dissolve and constant volume, make mass concentration and be respectively jasminoidin 0.6mg/mL, liquirtin 0.75mg/
ML, paeonol 0.8mg/mL, peoniflorin 1.2mg/mL, glycyrrhizic acid 0.8mg/mL, the mixing reference substance storage of ferulic acid 0.25mg/mL
Standby liquid, accurate respectively draw 2,4,6,8,12mL storing solution be placed in 25mL volumetric flask, flow phase constant volume, prepares series concentration
Mixing reference substance solution.
B. need testing solution is prepared: weighed the JIAWEI XIAOYAOWAN powder about 1.0g to be measured of 40 mesh sieves, accurately weighed, put
In 100mL ground tool plug conical flask, accurate addition absolute methanol 50mL, close plug, weighed weight, with power 200W, frequency 80kHz
Supersound process 30min, cooling, more weighed weight, supply the weight of less loss with absolute methanol, shake up, with 12000r/min rotating speed
It is centrifuged, takes supernatant, filter through 0.45 μm microporous filter membrane, obtain described need testing solution.
C. detect with high performance liquid chromatograph.Chromatographic condition: chromatographic column is Plastisil ODS C18, flowing be mutually
Acetonitrile 0.2% phosphoric acid water；Flow velocity is 1.0mL/min；Column temperature 37 DEG C；Sample size 10 μ L；Detector is photodiode array detection
Device, DAD reference wavelength is that described in 380nm, detection wavelength is 230nm, 238nm, 254nm, 274nm, 328nm, corresponding one-tenth to be measured
Be divided into: 230nm is peoniflorin, 238nm be jasminoidin, 254nm be glycyrrhizic acid, 274nm is paeonol and liquirtin, and 328nm is
Ferulic acid；Gradient elution during detection, gradient elution order:
0～10min, acetonitrile concentration is increased to 8% by 5%；
10～15min, acetonitrile concentration is increased to 10% by 8%；
15～20min, acetonitrile concentration is increased to 13% by 10%；
20～25min, acetonitrile concentration is increased to 16% by 13%；
25～28min, acetonitrile concentration is increased to 20% by 16%；
28～36min, acetonitrile concentration is increased to 22% by 20；
36～40min, acetonitrile concentration is 22%；
40～43min, acetonitrile concentration is increased to 27% by 22%；
43～51min, acetonitrile concentration is increased to 40% by 27%；
51～60min, acetonitrile concentration is increased to 50% by 40%；
60～70min, acetonitrile concentration is increased to 75% by 50%；
70～75min, acetonitrile concentration is increased to 90% by 75%；
75～80min, acetonitrile concentration is 90%.
Chromatogram is as shown in Figure 1.
D. the content of 6 kinds of compositions in described need testing solution is calculated: calculate described in described need testing solution with external standard method
Jasminoidin, liquirtin, paeonol, peoniflorin, glycyrrhizic acid, the content of 6 kinds of compositions of ferulic acid.Result of calculation is as indicated with 1.
In table 1 different batches JIAWEI XIAOYAOWAN 6 kinds of components to be measured assay result (N=3)
Embodiment 2
Carry out stability test, take with a collection of (lot number is: 20150811) need testing solution, in room temperature place 0,2,4,6,
8,12h, accurate absorption 10 μ L, as described in embodiment 1, chromatographic condition sample introduction is analyzed, and calculates the RSD value of each chromatographic peak area, result
The RSD value of jasminoidin, liquirtin, paeonol, peoniflorin, glycyrrhizic acid, ferulic acid each component peaks area is respectively less than 1.5%, shows
Need testing solution is good at 12h internal stability.
Embodiment 3
Carry out replica test, the multicomponent content assaying method of a kind of JIAWEI XIAOYAOWAN, take same
Batch (lot number is: 20150811) need testing solution, 5 parts of need testing solutions of parallel preparation, measures each component peaks area RSD value, tie
Really jasminoidin, liquirtin, paeonol, peoniflorin, glycyrrhizic acid, the RSD value of ferulic acid peak area are respectively less than 1.4%, show method
Repeatability is good.
Embodiment 4
Carrying out average recovery test, precision weighs the JIAWEI XIAOYAOWAN sample of known content, and (lot number is: 20150644) 6
Part, every part of 0.5g, it is placed in round-bottomed flask, is separately added into each reference substance appropriate, prepares need testing solution by step 2 method, fixed
Holding to 50mL, chromatographic condition as described in embodiment 1 is measured, and calculates the response rate of each composition respectively, the results are shown in Table 2.
Table 2 average recovery result of the test
Although, the present invention is described in detail to have used general explanation and specific embodiment, but at this
On the basis of invention, can make some modifications or improvements its design, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements without departing from theon the basis of the spirit of the present invention, each fall within the scope of protection of present invention.
Claims (9)
1. a multicomponent content assaying method for JIAWEI XIAOYAOWAN, it is characterized in that perform following steps:
A. reference substance solution is prepared: weigh a certain amount of jasminoidin, liquirtin, paeonol, peoniflorin, glycyrrhizic acid, Resina Ferulae respectively
Acid reference substance, is dissolved with solvent, is obtained certain density reference substance storing solution；Precision measures a certain amount of described reference substance respectively
Storing solution solubilizer dilutes, and obtains reference substance solution；
B. prepare need testing solution: JIAWEI XIAOYAOWAN to be measured be dissolved in solvent, filter, obtain certain density test sample molten
Liquid；
C. detecting with high performance liquid chromatograph, wherein said high performance liquid chromatograph includes DAD detector, chromatographic condition:
Chromatographic column is Plastisil ODS C18, acetonitrile phosphoric acid water be 0.8mL/min～1.2mL/min, column temperature for flowing phase, flow velocity
Be 35 DEG C～39 DEG C, detection wavelength be 200nm～400nm, sample size 5-20 μ L；
D. the content of 6 kinds of compositions in described need testing solution is calculated: calculate Fructus Gardeniae described in described need testing solution with external standard method
Glycosides, liquirtin, paeonol, peoniflorin, glycyrrhizic acid, the content of 6 kinds of compositions of ferulic acid.
2. such as the multicomponent content assaying method of claim 1 JIAWEI XIAOYAOWAN, it is characterized in that: described solvent is for without water beetle
Alcohol.
3. such as the multicomponent content assaying method of claim 2 JIAWEI XIAOYAOWAN, it is characterized in that: described detector is two poles
Pipe array detector, DAD reference wavelength is that described in 380nm, detection wavelength is 230nm, 238nm, 254nm, 274nm, 328nm, right
The composition to be measured answered is: 230nm is peoniflorin, 238nm be jasminoidin, 254nm be glycyrrhizic acid, 274nm is paeonol and Radix Glycyrrhizae
Glycosides, 328nm is ferulic acid.
4. the multicomponent content assaying method of a kind of JIAWEI XIAOYAOWAN as described in claim 14 any one, its feature
It is: described flowing is mutually for acetonitrile 0.1～0.2% phosphoric acid water.
5. the multicomponent content assaying method of a kind of JIAWEI XIAOYAOWAN as described in claim 14 any one, its feature
It is: described flowing is mutually for acetonitrile 0.2% phosphoric acid water；Described flow velocity is 1.0mL/min；Described column temperature 37 DEG C；Described sample size
10μL。
6. the multicomponent content assaying method of a kind of JIAWEI XIAOYAOWAN as described in claim 14 any one, its feature
It is: gradient elution when described high performance liquid chromatograph detects, gradient elution order:
0～10min, acetonitrile concentration is increased to 8% by 5%；
10～15min, acetonitrile concentration is increased to 10% by 8%；
15～20min, acetonitrile concentration is increased to 13% by 10%；
20～25min, acetonitrile concentration is increased to 16% by 13%；
25～28min, acetonitrile concentration is increased to 20% by 16%；
28～36min, acetonitrile concentration is increased to 22% by 20；
36～40min, acetonitrile concentration is 22%；
40～43min, acetonitrile concentration is increased to 27% by 22%；
43～51min, acetonitrile concentration is increased to 40% by 27%；
51～60min, acetonitrile concentration is increased to 50% by 40%；
60～70min, acetonitrile concentration is increased to 75% by 50%；
70～75min, acetonitrile concentration is increased to 90% by 75%；
75～80min, acetonitrile concentration is 90%.
The multicomponent content assaying method of a kind of JIAWEI XIAOYAOWAN the most as claimed in claim 1, is characterized in that: described in step B
The preparation method of need testing solution is:
Take 0.5～1.5g JIAWEI XIAOYAOWAN to be measured, accurately weighed, the accurate absolute methanol 25～75mL that adds, weighed weight, ultrasonic
Process 20～40min, let cool, supply the weight of less loss with absolute methanol, shake up, centrifugal, take supernatant, through 0.22～0.45 μm
Microporous filter membrane filters, and obtains described need testing solution.
The multicomponent content assaying method of a kind of JIAWEI XIAOYAOWAN the most as claimed in claim 1, is characterized in that: described in step B
The preparation method of need testing solution is:
Weighed the JIAWEI XIAOYAOWAN powder about 1.0g to be measured of 40 mesh sieves, accurately weighed, put in 100mL ground tool plug conical flask,
The accurate absolute methanol 50mL that adds, close plug, weighed weight, with power 200W, frequency 80kHz supersound process 30min, cool down, then
Weighed weight, supplies the weight of less loss, shakes up with absolute methanol, is centrifuged with 12000r/min rotating speed, takes supernatant, warp
0.45 μm microporous filter membrane filters, and obtains described need testing solution.
The multicomponent content assaying method of a kind of JIAWEI XIAOYAOWAN the most as claimed in claim 1, is characterized in that: described in step A
The preparation method of reference substance solution is:
Weigh jasminoidin, liquirtin, paeonol, peoniflorin, glycyrrhizic acid, ferulic acid reference substance respectively in right amount, add absolute methanol molten
Solve and constant volume, make mass concentration and be respectively jasminoidin 0.6mg/mL, liquirtin 0.75mg/mL, paeonol 0.8mg/mL, Radix Paeoniae
Glycosides 1.2mg/mL, glycyrrhizic acid 0.8mg/mL, the mixing reference substance storing solution of ferulic acid 0.25mg/mL, respectively precision draw 2,4,
6,8,12mL storing solution be placed in 25mL volumetric flask, flow phase constant volume, prepares the mixing reference substance solution of series concentration.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106841414A (en) * | 2016-12-14 | 2017-06-13 | 广西壮族自治区柳州食品药品检验所 | The detection method of Gardenoside and paeoniflorin content in a kind of chinaberry Cape jasmine oral liquid |
| CN108226359A (en) * | 2018-04-05 | 2018-06-29 | 盐城工业职业技术学院 | The method for measuring component content in ease pill |
| CN112345676A (en) * | 2020-12-07 | 2021-02-09 | 葵花药业集团湖北武当有限公司 | HPLC method for simultaneously detecting 6 active ingredients in fritillaria cirrhosa lung-heat-clearing syrup |
| CN114689712A (en) * | 2020-12-29 | 2022-07-01 | 四川新绿色药业科技发展有限公司 | Multi-component quality detection method for channel warming decoction extract |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634179A (en) * | 2003-12-26 | 2005-07-06 | 广州中医药大学 | Composition extracted from Danzhi Xiaoyao Powder and application thereof |
| US20120121696A1 (en) * | 2009-06-24 | 2012-05-17 | Zhijun Liu | Terpene Glycosides and Their Combinations as Solubilizing Agents |
| CN103800487A (en) * | 2014-02-25 | 2014-05-21 | 湖南天济草堂制药有限公司 | Moutan bark-cape jasmine medicine composition, preparation method and tablet thereof |
-
2016
- 2016-07-29 CN CN201610614520.0A patent/CN106053702B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634179A (en) * | 2003-12-26 | 2005-07-06 | 广州中医药大学 | Composition extracted from Danzhi Xiaoyao Powder and application thereof |
| US20120121696A1 (en) * | 2009-06-24 | 2012-05-17 | Zhijun Liu | Terpene Glycosides and Their Combinations as Solubilizing Agents |
| CN103800487A (en) * | 2014-02-25 | 2014-05-21 | 湖南天济草堂制药有限公司 | Moutan bark-cape jasmine medicine composition, preparation method and tablet thereof |
Non-Patent Citations (4)
| Title |
|---|
| 周欣 等: "高效液相色谱法测定丹栀逍遥丸中栀子苷、芍药苷、丹皮酚和甘草酸含量", 《药物分析杂志》 * |
| 李明月 等: "加味逍遥提取物超高效液相色谱指纹图谱研究", 《药物评价研究》 * |
| 杨永红: "丹栀逍遥散6种有效成分的HPLC测定", 《中国实验方剂学杂志》 * |
| 焦少珍 等: "RP-HPLC法同时测定加味逍遥口服液中栀子苷、芍药苷和丹皮酚的含量", 《湖北大学学报》 * |
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| CN106841414A (en) * | 2016-12-14 | 2017-06-13 | 广西壮族自治区柳州食品药品检验所 | The detection method of Gardenoside and paeoniflorin content in a kind of chinaberry Cape jasmine oral liquid |
| CN108226359A (en) * | 2018-04-05 | 2018-06-29 | 盐城工业职业技术学院 | The method for measuring component content in ease pill |
| CN112345676A (en) * | 2020-12-07 | 2021-02-09 | 葵花药业集团湖北武当有限公司 | HPLC method for simultaneously detecting 6 active ingredients in fritillaria cirrhosa lung-heat-clearing syrup |
| CN114689712A (en) * | 2020-12-29 | 2022-07-01 | 四川新绿色药业科技发展有限公司 | Multi-component quality detection method for channel warming decoction extract |
| CN114689712B (en) * | 2020-12-29 | 2023-10-03 | 四川新绿色药业科技发展有限公司 | Multi-component quality detection method for channel warming soup extract |
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