CN106053702B - A kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN - Google Patents
A kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN Download PDFInfo
- Publication number
- CN106053702B CN106053702B CN201610614520.0A CN201610614520A CN106053702B CN 106053702 B CN106053702 B CN 106053702B CN 201610614520 A CN201610614520 A CN 201610614520A CN 106053702 B CN106053702 B CN 106053702B
- Authority
- CN
- China
- Prior art keywords
- acetonitrile concentration
- acetonitrile
- reference substance
- concentration increase
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention belongs to Chinese medicine preparation Quality Control Technology fields, and in particular to a kind of JIAWEI XIAOYAOWAN content assaying method.Its technical solution is a kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN, including step：A. reference substance solution is prepared；B. test solution is prepared；C. it is detected, chromatographic condition with high performance liquid chromatograph (including DAD detectors)：Chromatographic column is Plastisil ODS C18, acetonitrile-phosphoric acid water be mobile phase, flow velocity is 0.8mL/min~1.2mL/min, column temperature is 35 DEG C~39 DEG C, Detection wavelength is 200nm~400nm, 5 20 μ L of sample size；D. the content of each ingredient is calculated.The present invention measures Gardenoside in JIAWEI XIAOYAOWAN simultaneously, liquiritin, Paeonol, Paeoniflorin, glycyrrhizic acid, the content of 6 kinds of ingredients of ferulic acid, easy to operate, high sensitivity, favorable reproducibility, and testing result is accurate and reliable.
Description
Technical field
The invention belongs to Chinese medicine preparation Quality Control Technology fields, and in particular to a kind of JIAWEI XIAOYAOWAN assay side
Method.
Background technology
JIAWEI XIAOYAOWAN is mainly by radix bupleuri, Radix Angelicae Sinensis, Radix Paeoniae Alba, cape jasmine, Rhizoma Atractylodis Macrocephalae, Poria cocos, Radix Glycyrrhizae, moutan bark, nine kinds of medicines of peppermint
Material forms, and is Traditional Chinese medicine historical preparation, has the effect of Shugan Qingre, strengthening spleen and nourishing blood, be clinically mainly used for hepatic depression and blood deficiency, liver and spleen
Discord, is had a dizzy spell, burnout deficiency of food, irregular menstruation, navel abdominal distention and pain etc. at two side of body distending pains.Wherein ferulic acid is Radix Angelicae Sinensis primary chemical
Ingredient；Paeoniflorin is the main chemical compositions of Radix Paeoniae Alba；Glycyrrhizic acid, the main chemical compositions that liquiritin is Radix Glycyrrhizae；Paeonol is male
The main feature ingredient of the root bark of tree peony；Gardenoside is the main chemical compositions of cape jasmine.
At present in Chinese Pharmacopoeia about only using Paeoniflorin a kind of in the assay item in the quality standard of JIAWEI XIAOYAOWAN
The content of ingredient controls the quality of the drug, it is difficult to really embody and ensure the inherent quality of JIAWEI XIAOYAOWAN.
It is also only on the books in existing literature：Gardenoside and Chinese herbaceous peony in hplc simultaneous determination JIAWEI XIAOYAOWAN
The content of glycosides；The content of Paeonol in high effective liquid chromatography for measuring JIAWEI XIAOYAOWAN；High effective liquid chromatography for measuring adds taste free and unfettered
The content of Paeoniflorin and liquiritin in distant ball.
It obviously can not thoroughly evaluating and control JIAWEI XIAOYAOWAN quality by 1-2 kinds chemical composition.But it can simply, accurately survey
Determine the more component contents or a technical barrier of JIAWEI XIAOYAOWAN.
Invention content
The purpose of the present invention is：In order to overcome deficiency in the prior art, the present invention provides a kind of the more of JIAWEI XIAOYAOWAN
Component content assay method, while measuring Gardenoside in JIAWEI XIAOYAOWAN, liquiritin, Paeonol, Paeoniflorin, glycyrrhizic acid, asafoetide
The content of sour 6 kinds of main components.
The technical scheme is that：A kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN, it is characterized in that execute with
Lower step：
A. reference substance solution is prepared：Weigh a certain amount of Gardenoside respectively, liquiritin, Paeonol, Paeoniflorin, glycyrrhizic acid,
Ferulic acid reference substance, is dissolved with solvent, obtains certain density reference substance storing solution；Accurate amount is a certain amount of described right respectively
Add absolute methanol to dilute according to product storing solution, obtains reference substance solution；
B. test solution is prepared：JIAWEI XIAOYAOWAN to be measured is dissolved in solvent, is filtered, is obtained certain density for examination
Product solution；
C. it is detected with high performance liquid chromatograph, wherein the high performance liquid chromatograph includes DAD detectors, chromatostrip
Part：Chromatographic column is Plastisil ODS C18, acetonitrile-phosphoric acid water be mobile phase, flow velocity be 0.8mL/min~1.2mL/min,
Column temperature is 35 DEG C~39 DEG C, Detection wavelength is 200nm~400nm, sample size 5-20 μ L；
D. the content of 6 kinds of ingredients in the test solution is calculated：It is calculated described in the test solution with external standard method
The content of Gardenoside, liquiritin, Paeonol, Paeoniflorin, 6 kinds of glycyrrhizic acid, ferulic acid ingredients.
Further, the solvent is absolute methanol.
Further, the detector is diode array detector, and DAD reference wavelengths are Detection wavelength described in 380nm
For 230nm, 238nm, 254nm, 274nm, 328nm, corresponding ingredient to be measured is：230nm is Paeoniflorin, 238nm be Gardenoside,
254nm is glycyrrhizic acid, and 274nm is Paeonol and liquiritin, and 328nm is ferulic acid.
Further, the mobile phase is 0.1~0.2% phosphoric acid water of acetonitrile-；
Further, the mobile phase is 0.2% phosphoric acid water of acetonitrile-；The flow velocity is 1.0mL/min；The column temperature 37
℃；10 μ L of the sample size
Further, the gradient elution when high performance liquid chromatograph is detected, gradient elution sequence：
0~10min, acetonitrile concentration increase to 8% by 5%；
10~15min, acetonitrile concentration increase to 10% by 8%；
15~20min, acetonitrile concentration increase to 13% by 10%；
20~25min, acetonitrile concentration increase to 16% by 13%；
25~28min, acetonitrile concentration increase to 20% by 16%；
28~36min, acetonitrile concentration increase to 22% by 20；
36~40min, acetonitrile concentration 22%；
40~43min, acetonitrile concentration increase to 27% by 22%；
43~51min, acetonitrile concentration increase to 40% by 27%；
51~60min, acetonitrile concentration increase to 50% by 40%；
60~70min, acetonitrile concentration increase to 75% by 50%；
70~75min, acetonitrile concentration increase to 90% by 75%；
75~80min, acetonitrile concentration 90%.
Further, the preparation method of test solution described in step B is：
0.5~1.5g JIAWEI XIAOYAOWANs to be measured are taken, accurately weighed, precision addition 25~75mL of absolute methanol, weighed weight,
It is ultrasonically treated 20~40min, lets cool, the weight of less loss is supplied with absolute methanol, is shaken up, centrifuges, takes supernatant, through 0.22~
0.45 μm of miillpore filter filtration, obtains the test solution.
Further, the preparation method of test solution described in step B is：
The JIAWEI XIAOYAOWAN powder about 1.0g to be measured of 40 mesh sieve was weighed, it is accurately weighed, set 100mL ground conical flask with cover
In, absolute methanol 50mL, close plug is added in precision, and weighed weight is ultrasonically treated 30min with power 200W, frequency 80kHz, cooling,
Weighed weight again is supplied the weight of less loss with absolute methanol, shaken up, and is centrifuged with 12000r/min rotating speeds, and supernatant is taken, warp
0.45 μm of miillpore filter filtration, obtains the test solution.
Further, the preparation method of reference substance solution described in step A is：
It is appropriate that Gardenoside, liquiritin, Paeonol, Paeoniflorin, glycyrrhizic acid, ferulic acid reference substance are weighed respectively, add no water beetle
Alcohol dissolving and constant volume, it is respectively 0.6 mg/mL of Gardenoside, liquiritin 0.75mg/mL, Paeonol 0.8mg/ that mass concentration, which is made,
ML, Paeoniflorin 1.2mg/mL, glycyrrhizic acid 0.8mg/mL, ferulic acid 0.25mg/mL mixing reference substance storing solution, it is accurate respectively to inhale
Take 2,4,6,8,12mL storing solutions be placed in 25mL volumetric flasks, the mixed reference substance solution of series concentration is made in mobile phase constant volume.
Beneficial effects of the present invention mainly have：The present invention is using high performance liquid chromatography (HPLC) under same chromatographic condition
Measure Gardenoside in JIAWEI XIAOYAOWAN simultaneously, liquiritin, Paeonol, Paeoniflorin, glycyrrhizic acid, the content of 6 kinds of ingredients of ferulic acid adopts
With gradient elution, a chromatography is completed in 80min, mean sample recovery rate 99.13%~100.25%, RSD values are equal<
2.0%.This method is easy to operate, high sensitivity, favorable reproducibility, testing result are accurate and reliable, is commented for the quality of JIAWEI XIAOYAOWAN
Valence provides a kind of effective detection method, can be used for quality control, the quality evaluation of JIAWEI XIAOYAOWAN, is quality evaluation
Raising with quality standard provides technical support.
Description of the drawings
The ultraviolet spectrogram of each ingredient to be measured under each Detection wavelengths of Fig. 1：A- Gardenoside uv-spectrograms；The ultraviolet figure of B- liquiritins
Spectrum；C- Paeonol uv-spectrograms；D- Paeoniflorin uv-spectrograms；E- glycyrrhizic acid uv-spectrograms；F- ferulic acid uv-spectrograms.
Specific implementation mode
Technical solution of the present invention is more clearly described in order to more intuitive, and helps to understand tribute of the present invention to the prior art
Place is offered, the present invention is described in detail below in conjunction with drawings and examples.
Embodiment 1
The present embodiment is a kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN, and selected instrument and material are as follows：
1260 type high performance liquid chromatographs (including DAD detectors, quarternary low pressure gradient pump, online vacuum degassing machine),
ChemStation work stations (Agilent companies of the U.S.), (ten a ten thousandths, Japanese Shimadzu are accurate for AUW220D type analysis balance
Instrumental Analysis company), KQ-250DE types ultrasonic cleaner (Fauna of Kunshan, Jiangsu ultrasonic instrument Co., Ltd)
Gardenoside (lot number：MUST-15022411, purity 98.13%)；Ferulic acid (lot number：MUST-14102214, purity
99.36%)；Liquiritin (lot number：MUST-14100910, purity 99.83%)；Paeoniflorin (lot number：MUST-15060112 is pure
Degree is 99.42%)；Glycyrrhizic acid (lot number：MUST-14091708, purity 98.69%)；Paeonol (lot number：MUST-15020401,
Purity 99.30%)；Above-mentioned reference substance is purchased from the Chengdu bio tech ltd Man Site；Acetonitrile (lot number：20150833, color
Compose pure), methanol (lot number：20150322, chromatographically pure) it is provided by Shandong YuWang Industry Co., Ltd；Water is that commercially available Wahaha is pure
Water, other reagents are that analysis is pure；
JIAWEI XIAOYAOWAN (lot number：20150644,20150811,20150810, specification：6g/ bags) by Beijing Tongrentang system
Medicine limited liability company provides；JIAWEI XIAOYAOWAN (lot number：1106208,1105122,1105803, specification：6g/ bags) by Tianjin
Its scholar's power (Liaoning) Pharmaceutical Co provides.
It is specific to execute step or less：
A. reference substance solution is prepared：Gardenoside, liquiritin, Paeonol, Paeoniflorin, glycyrrhizic acid, ferulic acid pair are weighed respectively
It is appropriate according to product, add absolute methanol dissolving and constant volume, it is respectively Gardenoside 0.6mg/mL, liquiritin 0.75mg/ that mass concentration, which is made,
ML, Paeonol 0.8mg/mL, Paeoniflorin 1.2mg/mL, the mixing reference substance storage of glycyrrhizic acid 0.8mg/mL, ferulic acid 0.25mg/mL
Standby liquid, it is accurate respectively draw 2,4,6,8,12mL storing solutions be placed in 25mL volumetric flasks, series concentration is made in mobile phase constant volume
Mixed reference substance solution.
B. test solution is prepared：The JIAWEI XIAOYAOWAN powder about 1.0g to be measured of 40 mesh sieve was weighed, it is accurately weighed, it sets
In 100mL ground conical flask with cover, absolute methanol 50mL, close plug, weighed weight, with power 200W, frequency 80kHz is added in precision
It is ultrasonically treated 30min, it is cooling, then weighed weight, the weight of less loss is supplied with absolute methanol, is shaken up, with 12000r/min rotating speeds
It is centrifuged, takes supernatant, filtered through 0.45 μm of miillpore filter, obtain the test solution.
C. it is detected with high performance liquid chromatograph.Chromatographic condition：Chromatographic column is Plastisil ODS C18, mobile phase be
0.2% phosphoric acid water of acetonitrile-；Flow velocity is 1.0mL/min；37 DEG C of column temperature；10 μ L of sample size；Detector is Diode Array Detector
Device, it is 230nm, 238nm, 254nm, 274nm, 328nm that DAD reference wavelengths, which are Detection wavelength described in 380nm, it is corresponding it is to be measured at
It is divided into：230nm is Paeoniflorin, and 238nm is Gardenoside, 254nm is glycyrrhizic acid, and 274nm is Paeonol and liquiritin, and 328nm is
Ferulic acid；Gradient elution when detection, gradient elution sequence：
0~10min, acetonitrile concentration increase to 8% by 5%；
10~15min, acetonitrile concentration increase to 10% by 8%；
15~20min, acetonitrile concentration increase to 13% by 10%；
20~25min, acetonitrile concentration increase to 16% by 13%；
25~28min, acetonitrile concentration increase to 20% by 16%；
28~36min, acetonitrile concentration increase to 22% by 20；
36~40min, acetonitrile concentration 22%；
40~43min, acetonitrile concentration increase to 27% by 22%；
43~51min, acetonitrile concentration increase to 40% by 27%；
51~60min, acetonitrile concentration increase to 50% by 40%；
60~70min, acetonitrile concentration increase to 75% by 50%；
70~75min, acetonitrile concentration increase to 90% by 75%；
75~80min, acetonitrile concentration 90%.
Chromatogram is as shown in Figure 1.
D. the content of 6 kinds of ingredients in the test solution is calculated：It is calculated described in the test solution with external standard method
The content of Gardenoside, liquiritin, Paeonol, Paeoniflorin, 6 kinds of glycyrrhizic acid, ferulic acid ingredients.Result of calculation is as indicated with 1.
In 1 different batches JIAWEI XIAOYAOWAN of table 6 kinds of components to be measured assay result (N=3)
Embodiment 2
Stability test is carried out, takes that (lot number is with a batch：20150811) test solution, in being placed at room temperature for 0,2,4,6,
8,12h, precision draws 10 μ L, and by described in embodiment 1, sample introduction is analyzed for chromatographic condition, calculates the RSD values of each chromatographic peak area, as a result
Gardenoside, liquiritin, Paeonol, Paeoniflorin, glycyrrhizic acid, ferulic acid each component peak area RSD values be respectively less than 1.5%, show
Test solution is good in 12h internal stabilities.
Embodiment 3
Repetitive test is carried out, a kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN as described in Example 1 takes same
Batch (lot number is：20150811) test solution, 5 parts of test solutions of parallel preparation measure each component peak area RSD values, knot
Fruit Gardenoside, liquiritin, Paeonol, Paeoniflorin, glycyrrhizic acid, forulic acid peak area RSD values be respectively less than 1.4%, show method
It is repeated good.
Embodiment 4
Carry out sample recovery rate experiment, precision weighs the JIAWEI XIAOYAOWAN sample of known content, and (lot number is：20150644)6
Part, every part of 0.5g is placed in round-bottomed flask, and it is appropriate to be separately added into each reference substance, and test solution is prepared by step 2 method, fixed
Hold to 50mL, is measured by chromatographic condition described in embodiment 1, and calculate separately the rate of recovery of each ingredient, the results are shown in Table 2.
2 sample recovery rate test result of table
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be conceived and make some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, each fall within the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (4)
1. the multicomponent content assaying method of a kind of JIAWEI XIAOYAOWAN, it is characterized in that executing following steps：
A. reference substance solution is prepared：A certain amount of Gardenoside, liquiritin, Paeonol, Paeoniflorin, glycyrrhizic acid, asafoetide are weighed respectively
Sour reference substance, is dissolved with solvent, obtains certain density reference substance storing solution；It is accurate respectively to measure a certain amount of reference substance
Storing solution solubilization dilution agent, obtains reference substance solution；
B. test solution is prepared：JIAWEI XIAOYAOWAN to be measured is dissolved in solvent, filters, it is molten to obtain certain density test sample
Liquid；
C. it is detected with high performance liquid chromatograph, wherein the high performance liquid chromatograph includes DAD detectors, chromatographic condition：
Chromatographic column is Plastisil ODS C18, acetonitrile-phosphoric acid water be mobile phase, flow velocity is 0.8mL/min~1.2mL/min, column temperature
For 35 DEG C~39 DEG C, Detection wavelength be 200nm~400nm, sample size 5-20 μ L；
D. the content of 6 kinds of ingredients in the test solution is calculated：Cape jasmine described in the test solution is calculated with external standard method
The content of glycosides, liquiritin, Paeonol, Paeoniflorin, 6 kinds of glycyrrhizic acid, ferulic acid ingredients；
Solvent described in step A is absolute methanol；
Detector described in step C be diode array detector, DAD reference wavelengths be Detection wavelength described in 380nm be 230nm,
238nm, 254nm, 274nm, 328nm, corresponding ingredient to be measured are：230nm is Paeoniflorin, and 238nm is Gardenoside, 254nm is
Glycyrrhizic acid, 274nm are Paeonol and liquiritin, and 328nm is ferulic acid；The mobile phase is 0.1~0.2% phosphoric acid of acetonitrile-
Water；
Mobile phase described in step C is 0.2% phosphoric acid water of acetonitrile-；The flow velocity is 1.0mL/min；37 DEG C of the column temperature；It is described
10 μ L of sample size；
Gradient elution when high performance liquid chromatograph described in step C is detected, gradient elution sequence：
0~10min, acetonitrile concentration increase to 8% by 5%；
10~15min, acetonitrile concentration increase to 10% by 8%；
15~20min, acetonitrile concentration increase to 13% by 10%；
20~25min, acetonitrile concentration increase to 16% by 13%；
25~28min, acetonitrile concentration increase to 20% by 16%；
28~36min, acetonitrile concentration increase to 22% by 20；
36~40min, acetonitrile concentration 22%；
40~43min, acetonitrile concentration increase to 27% by 22%；
43~51min, acetonitrile concentration increase to 40% by 27%；
51~60min, acetonitrile concentration increase to 50% by 40%；
60~70min, acetonitrile concentration increase to 75% by 50%；
70~75min, acetonitrile concentration increase to 90% by 75%；
75~80min, acetonitrile concentration 90%.
2. a kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN as described in claim 1, it is characterized in that：Described in step B
The preparation method of test solution is：
0.5~1.5g JIAWEI XIAOYAOWANs to be measured are taken, accurately weighed, 25~75mL of absolute methanol, weighed weight, ultrasound is added in precision
20~40min is handled, lets cool, the weight of less loss is supplied with absolute methanol, is shaken up, centrifuges, supernatant is taken, through 0.22~0.45 μm
Miillpore filter filters, and obtains the test solution.
3. a kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN as described in claim 1, it is characterized in that：Described in step B
The preparation method of test solution is：
The JIAWEI XIAOYAOWAN powder about 1.0g to be measured of 40 mesh sieve was weighed, it is accurately weighed, it sets in 100mL ground conical flask with cover,
Absolute methanol 50mL, close plug is added in precision, and weighed weight is ultrasonically treated 30min with power 200W, frequency 80kHz, cooling, then
Weighed weight is supplied the weight of less loss with absolute methanol, shaken up, and is centrifuged with 12000r/min rotating speeds, and supernatant is taken, warp
0.45 μm of miillpore filter filtration, obtains the test solution.
4. a kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN as described in claim 1, it is characterized in that：Described in step A
The preparation method of reference substance solution is：
It is appropriate that Gardenoside, liquiritin, Paeonol, Paeoniflorin, glycyrrhizic acid, ferulic acid reference substance are weighed respectively, add absolute methanol molten
Simultaneously constant volume is solved, it is respectively Gardenoside 0.6mg/mL, liquiritin 0.75mg/mL, Paeonol 0.8mg/mL, Chinese herbaceous peony that mass concentration, which is made,
The mixing reference substance storing solution of glycosides 1.2mg/mL, glycyrrhizic acid 0.8mg/mL, ferulic acid 0.25mg/mL, it is accurate respectively draw 2,4,
6,8,12mL storing solutions are placed in 25mL volumetric flasks, mobile phase constant volume, and the mixed reference substance solution of series concentration is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610614520.0A CN106053702B (en) | 2016-07-29 | 2016-07-29 | A kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610614520.0A CN106053702B (en) | 2016-07-29 | 2016-07-29 | A kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106053702A CN106053702A (en) | 2016-10-26 |
| CN106053702B true CN106053702B (en) | 2018-08-03 |
Family
ID=57196949
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610614520.0A Active CN106053702B (en) | 2016-07-29 | 2016-07-29 | A kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106053702B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106841414A (en) * | 2016-12-14 | 2017-06-13 | 广西壮族自治区柳州食品药品检验所 | The detection method of Gardenoside and paeoniflorin content in a kind of chinaberry Cape jasmine oral liquid |
| CN108226359A (en) * | 2018-04-05 | 2018-06-29 | 盐城工业职业技术学院 | The method for measuring component content in ease pill |
| CN112345676B (en) * | 2020-12-07 | 2022-06-07 | 葵花药业集团湖北武当有限公司 | HPLC method for simultaneously detecting 6 active ingredients in fritillaria cirrhosa lung-heat-clearing syrup |
| CN114689712B (en) * | 2020-12-29 | 2023-10-03 | 四川新绿色药业科技发展有限公司 | Multi-component quality detection method for channel warming soup extract |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634179A (en) * | 2003-12-26 | 2005-07-06 | 广州中医药大学 | Composition extracted from Danzhi Xiaoyao Powder and application thereof |
| CN103800487A (en) * | 2014-02-25 | 2014-05-21 | 湖南天济草堂制药有限公司 | Moutan bark-cape jasmine medicine composition, preparation method and tablet thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2445535A4 (en) * | 2009-06-24 | 2014-12-17 | Univ Louisiana State | Terpene glycosides and their combinations as solubilizing agents |
-
2016
- 2016-07-29 CN CN201610614520.0A patent/CN106053702B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634179A (en) * | 2003-12-26 | 2005-07-06 | 广州中医药大学 | Composition extracted from Danzhi Xiaoyao Powder and application thereof |
| CN103800487A (en) * | 2014-02-25 | 2014-05-21 | 湖南天济草堂制药有限公司 | Moutan bark-cape jasmine medicine composition, preparation method and tablet thereof |
Non-Patent Citations (4)
| Title |
|---|
| RP-HPLC法同时测定加味逍遥口服液中栀子苷、芍药苷和丹皮酚的含量;焦少珍 等;《湖北大学学报》;20070630;第29卷;196-198页 * |
| 丹栀逍遥散6种有效成分的HPLC测定;杨永红;《中国实验方剂学杂志》;20140831;第20卷;97-99页 * |
| 加味逍遥提取物超高效液相色谱指纹图谱研究;李明月 等;《药物评价研究》;20160430;第39卷(第2期);第230页"摘要"，第230页首段，第231页第1-2节，第234页图3、4，第236页左栏第1-3段 * |
| 高效液相色谱法测定丹栀逍遥丸中栀子苷、芍药苷、丹皮酚和甘草酸含量;周欣 等;《药物分析杂志》;20051231;第25卷(第7期);第784页"摘要"，第1-2段，第784-785页1-5节，表1，第786页图1，第11节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106053702A (en) | 2016-10-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106053702B (en) | A kind of multicomponent content assaying method of JIAWEI XIAOYAOWAN | |
| CN107860832B (en) | Method for establishing fingerprint of compound rhubarb clear pancreas soup | |
| CN103207255B (en) | A kind of detection method of content of NAOXINTONG JIAONANG | |
| CN103575819A (en) | Method for measuring fingerprint spectrum of cardiac traditional Chinese medicine preparation | |
| CN105353063B (en) | Compound cold drug stream livering ingredient standard finger-print and construction method, application | |
| CN106290599A (en) | A kind of content assaying method of Chinese medicine composition | |
| CN103808835A (en) | Method for simultaneously measuring contents of 10 chemical components in four-substance soup decoction by HPLC-DAD (high performance liquid chromatography-diode array detection) method | |
| CN106370756B (en) | A kind of detection method of Chinese materia medica preparation that preventing and treating infectious bronchitis of chicken | |
| CN107238671A (en) | The content assaying method of Multiple components in a kind of Zhenqi Fuzheng prepn | |
| CN106501396A (en) | A kind of detection method of hemorrhoid medicine index components content | |
| CN113759035A (en) | Method for constructing fingerprint of Xiaochengqi decoction | |
| CN104007198B (en) | A kind of glossy ganoderma emperor's preparation HPLC standard finger-print and construction method thereof and application | |
| CN104155383B (en) | The detection method of blue or green Pu granule | |
| CN105510452B (en) | Multi-target ingredient assay, fingerprint map construction and the preparation method of liver-benefiting eye-brightening oral liquid | |
| CN103604898B (en) | The benefit heart relaxes the fingerprint atlas detection method of preparation | |
| CN110274970A (en) | The method for building up for melting poor finger-print and its application in Yixiesheng capsule Quality Control | |
| CN105842381A (en) | Detection method of Qigu capsule | |
| CN105301140B (en) | A kind of injection component detection method promoting blood circulation and removing obstruction in channels | |
| CN112345679B (en) | Fingerprint characteristic spectrum of Wuling preparation, establishing method and application thereof | |
| CN112526045B (en) | Method for simultaneously detecting or identifying effective components in heart-soothing and lipid-lowering tablets | |
| CN107884489A (en) | A kind of detection method of Zaizao Pill | |
| CN108614066A (en) | A kind of Traditional Chinese medicine composition detection method for treating coronary heart disease | |
| CN104597168B (en) | Ramulus Mori refines decoction pieces content assaying method | |
| CN106706810B (en) | Novel biochemical particles quantitative finger print atlas detection method and its finger-print | |
| CN105203469A (en) | Detection method of medicament for treating femoral head necrosis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |