CN104020235A - Method for simultaneously determining content of chlorogenic acid and galuteolin in lonicera japonica - Google Patents
Method for simultaneously determining content of chlorogenic acid and galuteolin in lonicera japonica Download PDFInfo
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- CN104020235A CN104020235A CN201410292908.4A CN201410292908A CN104020235A CN 104020235 A CN104020235 A CN 104020235A CN 201410292908 A CN201410292908 A CN 201410292908A CN 104020235 A CN104020235 A CN 104020235A
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Abstract
The invention provides a method for simultaneously determining the content of chlorogenic acid and galuteolin in lonicera japonica. The method comprises ultrasonic extraction, quantitative analysis and the like. In an ultrasonic extraction process, the solid-liquid ratio of lonicera japonica powder to 70% ethanol water solution ranges from (1:80) to (1:120); the extraction time is 45-60 minutes. UPLC (Ultra Performance Liquid Chromatography) conditions are as follows: a chromatographic column: Waters Acquity UPLC BEH RP18; a mobile phase: acetonitrile-0.4% phosphoric acid water; elution gradient: 0-2 minutes and 5%-16% B; 2-4 minutes and 16%-20% B; 4-8 minutes and 20%-50% B; 8-10 minutes and 50%-100% B; 10-11 minutes and 100%-5% B; detection wavelength: 242nm. The method is simple and can be used for simultaneously determining the content of chlorogenic acid and galuteolin in lonicera japonica; the method is high in sensitivity, high in precision and accuracy, and good in repeatability, and is stable and reliable.
Description
Technical field
The present invention relates to the detection method of effective ingredient of honeysuckle, relate more specifically to a kind of method that effective constituent-chlorogenic acid to honeysuckle simultaneously and galuteolin carry out quantitative test.
Background technology
Honeysuckle is the dry flower of caprifoliaceae plant honeysuckle Lonicera japonica Thunb. or the flower that band is just opened, and traditional Chinese medicine has effect of clearing heat and detoxicating, dispelling wind and heat from the body, antiviral, hepatic cholagogic.Honeysuckle main product is in the ground such as Henan, Shandong, and its main chemical compositions is organic acid, triterpenes, flavonoids and volatilization wet goods, and wherein Determination of Organic Acids is antibacterial, the antiviral active component of honeysuckle.Chlorogenic acid is one of main effective constituent of honeysuckle, not only as the quality control index of its crude drug, is also the quality control index of some patent medicine and preparation.In 2010 editions pharmacopeia, taking chlorogenic acid and galuteolin as index composition, honeysuckle is carried out to quality control.
At present technology and the means of chlorogenic acid and galuteolin quantitative test are mainly contained: thin layer chromatography scanning (TLCS), capillary electrophoresis (CE), high performance liquid chromatography (HPLC), liquid-matter coupling technique (LC-MS) etc., wherein universal with HPLC method.Ultra Performance Liquid Chromatography method (UPLC) is that (particle diameter is generally less than 2 μ m) and a kind of isolation technics of UHV (ultra-high voltage) (pressure is greater than 105KPa) system to employing granule filler chromatographic column, with respect to HPLC, it more can significantly improve degree of separation and the detection sensitivity of chromatographic peak, simultaneously can shorten analysis time, reduce the use etc. of organic reagent, be widely used in the constituent analysis of Chinese herbal medicine at present.UPLC has been reported in the quantitative test of Chlorogenic Acid of Flos Lonicerae, but how quick, and the method for simultaneously measuring exactly Chlorogenic Acid of Flos Lonicerae and two kinds of component contents of galuteolin need research.
Summary of the invention
For the problems referred to above, the object of this invention is to provide a kind of accurately, fast and can measure the method for the content of Chlorogenic Acid of Flos Lonicerae and galuteolin simultaneously.
The method of simultaneously measuring Chlorogenic Acid of Flos Lonicerae and galuteolin content of the present invention, comprises the following steps:
Ultrasound wave extracts: extracting honeysuckle powder sieves, according to 1: 80-1: 120 solid-to-liquid ratio, add 70% ethanol water, weighed weight, ultrasonic extraction 45-60min, lets cool, and obtains Flos Lonicerae extractive solution.
Quantitative test: utilize UPLC-PDA method to carry out quantitative test, wherein UPLC condition: chromatographic column is Waters Acquity UPLC BEH RP
18(100mm × 2.1mm i.d., 1.7 μ are m); Room temperature, mobile phase: A:B=0.4% phosphoric acid water: acetonitrile; Condition of gradient elution: equilibration time is 5min; Gradient elution program: 0～2min, 5%～16%B; 2～4min, 16%～20%B; 4～8min, 20%～50%B; 8～10min, 50%～100%B; 10～11min, 100%～5%B; Sample size: 3 μ L, detect wavelength: 242nm, volumetric flow rate 0.2mL/min.
Preferably, in ultrasound wave extraction step, Honeysuckle Flower, according to the solid-to-liquid ratio of 1: 120, adds 70% ethanol water, weighed weight, and ultrasonic extraction 45min, lets cool, and obtains Flos Lonicerae extractive solution.
Preferably, in quantitative test step, also comprise: the preparation of reference substance solution: precision takes chlorogenic acid, galuteolin reference substance: 0.889mg, 0.920mg, taking acetonitrile-0.4% phosphate aqueous solution as solvent, in brown volumetric flask, be settled to 2mL, as storing solution.The reference substance solution of other different quality concentration is obtained by storing solution dilution.
Preferably, in ultrasound wave extraction step, Honeysuckle Flower is crossed 65-80 mesh sieve.
Preferably, before quantitative test, carry out the preparation of need testing solution, comprising: extracting honeysuckle extract 70% ethanol water is supplied weight, shake up, filter, cross 0.22 μ m miillpore filter, obtain need testing solution.
The method of simultaneously measuring Chlorogenic Acid of Flos Lonicerae and galuteolin content of the present invention, method is simple, can measure the content of Chlorogenic Acid of Flos Lonicerae and galuteolin simultaneously, have highly sensitive, preci-sion and accuracy is high, reproducible, reliable and stable advantage.
1, method is simple, and the time is short, in the present invention, adopt ultrasound wave to extract, simple to operate, efficiency is high, in described ultrasound wave extraction step, select 70% ethanol water greatly to improve extraction efficiency, shortened extraction time, inventor studies discovery, selects 70% ethanol water as extracting solvent, extraction time only needs 45min, has greatly shortened extraction time.
2, extraction efficiency is high, and testing result is accurate, and in ultrasound wave extraction step, Honeysuckle Flower, according to the solid-to-liquid ratio of 1: 120, adds 70% ethanol water, weighed weight.Inventor studies discovery, when the solid-to-liquid ratio of the ethanol water of Honeysuckle Flower and 70% is during lower than 1: 120, Flos Lonicerae extractive solution Content of Chlorogenic Acid has overload phenomenon, when the solid-to-liquid ratio of the ethanol water of Honeysuckle Flower and 70% is during higher than 1: 120, the response of galuteolin is lower, affects testing result.
3, the method of simultaneously measuring Chlorogenic Acid of Flos Lonicerae and galuteolin content of the present invention, utilize Ultra Performance Liquid Chromatography method (UPLC), Ultra Performance Liquid Chromatography method (UPLC) is that (particle diameter is generally less than 2 μ m) and a kind of isolation technics of UHV (ultra-high voltage) (pressure is greater than 105KPa) system to employing granule filler chromatographic column, with respect to HPLC, it more can significantly improve degree of separation and the detection sensitivity of chromatographic peak, can shorten analysis time simultaneously, reduce the use etc. of organic reagent.
4, the present invention utilizes UPLC-PDA method to measure the content of Chlorogenic Acid of Flos Lonicerae and galuteolin simultaneously, and its chromatographic condition adopting can be realized within shorter analysis time, makes two kinds of materials reach good separation.When gradient elution, the chromatogram baseline that the phosphate aqueous solution of mobile phase employing 0.4% obtains is steady, and the degree of separation of chlorogenic acid and galuteolin is good; Use RP18 post separating effect better; In the time that wavelength is 242nm, its response can accurate response chlorogenic acid and the content of galuteolin.
The method of simultaneously measuring Chlorogenic Acid of Flos Lonicerae and galuteolin content of the present invention can be measured the content of Chlorogenic Acid of Flos Lonicerae and galuteolin simultaneously, and method accurately, fast, provide reliable evaluation method for the quality control of honeysuckle.
Brief description of the drawings
Fig. 1 is one of them sample of method of Chlorogenic Acid of Flos Lonicerae and galuteolin content and the UPLC-PDA chromatogram of reference substance simultaneously measured of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail, to make those skilled in the art can implement according to this with reference to instructions word.
The method of simultaneously measuring Chlorogenic Acid of Flos Lonicerae and galuteolin content of the present invention, comprises the following steps:
Ultrasound wave extracts: extracting honeysuckle powder sieves, according to 1: 80-1: 120 solid-to-liquid ratio, add 70% ethanol water, weighed weight, ultrasonic extraction 45-60min, lets cool, and obtains Flos Lonicerae extractive solution.
Quantitative test: extracting honeysuckle extract is supplied weight with 70% ethanol water, shakes up, and filters, and crosses 0.22 μ m miillpore filter, obtains need testing solution;
UPLC condition: chromatographic column is Waters Acquity UPLC BEH RP
18(100mm × 2.1mm i.d., 1.7 μ are m); Room temperature, mobile phase: A:B=0.4% phosphoric acid water: acetonitrile; Condition of gradient elution: equilibration time is 5min; Gradient elution program: 0～2min, 5%～16%B; 2～4min, 16%～20%B; 4～8min, 20%～50%B; 8～10min, 50%～100%B; 10～11min, 100%～5%B; Sample size: 3 μ L, detect wavelength: 242nm, volumetric flow rate 0.2mL/min.
Wherein, in ultrasound wave extraction step, Honeysuckle Flower, according to the solid-to-liquid ratio of 1: 120, adds 70% ethanol water, weighed weight, and ultrasonic extraction 45min, lets cool, and obtains Flos Lonicerae extractive solution.
Wherein, in quantitative test step, also comprise: the preparation of reference substance solution: precision takes chlorogenic acid, galuteolin reference substance: 0.889mg, 0.920mg taking acetonitrile-0.4% phosphate aqueous solution as solvent, is settled to 2mL, as storing solution in brown volumetric flask.The reference substance solution of other different quality concentration is obtained by storing solution dilution.
Wherein, in ultrasound wave extraction step, Honeysuckle Flower is crossed 65-80 mesh sieve.
Embodiment 1
The method of the content of simultaneously measuring Chlorogenic Acid of Flos Lonicerae and galuteolin of the present invention, the instrument of employing and material:
Waters Acquity UPLC H-Class system, comprises quaternary pump, the online degasser of vacuum, automatic sampler, PDA detecting device, Empower workstation.100000/balance (Mei Tele company); EL204 ten thousand/balance (Shanghai exact instrument company limited); KQ-250DE ultrasonic cleaner (ultrasonic instrument company limited of city of Kunshan).
Methyl alcohol and acetonitrile (chromatographically pure, Fisher company), ethanol, phosphoric acid (analyze pure, Beijing Chemical Plant), distilled water.Reference substance chlorogenic acid (NO:110753-201314) and galuteolin (NO:111720-201106) are purchased from National Institute for Food and Drugs Control.Honeysuckle material source, in table 3, is accredited as the dry flower of caprifoliaceae plant honeysuckle Lonicera japonica Thunb. through Medicinal Plants of Guangxi garden Wu Qinghua assistant researcher.
The method of the content of simultaneously measuring Chlorogenic Acid of Flos Lonicerae and galuteolin of the present invention, comprises the following steps:
(1) preparation of reference substance solution: precision takes chlorogenic acid, galuteolin reference substance: 0.889mg, 0.920mg taking acetonitrile-0.4% phosphate aqueous solution as solvent, is settled to 2mL, as storing solution in brown volumetric flask.The reference substance solution of other different quality concentration is obtained by storing solution dilution.
(2) preparation of need testing solution: the about 0.25g of extracting honeysuckle powder (crossing 60 mesh sieves), accurately weighed, be placed in tool plug conical flask, precision adds 70% ethanol water 25mL, weighed weight, ultrasonic extraction (power 250W, frequency 40HZ) 45min, let cool, supply the weight of minimizing with 70% ethanol water, shake up, filter, get subsequent filtrate appropriate, cross 0.22 μ m miillpore filter, to obtain final product.
(3) chromatographic condition: chromatographic column is Waters Acquity UPLC BEH RP
18(100mm × 2.1mm i.d., 1.7 μ are m); Room temperature, mobile phase is 0.4% phosphoric acid water (A)-acetonitrile (B), gradient elution, equilibration time is 5min, gradient elution program: 0～2min, 5%～16%B; 2～4min, 16%～20%B; 4～8min, 20%～50%B; 8～10min, 50%～100%B; 10～11min, 100%～5%B; Sample size: 3 μ L, detect wavelength: 242nm, volumetric flow rate 0.2mL/min.
(4) sample determination
The traditional Chinese medicine honeysuckle powder of getting respectively 10 batches of different places of production, utilizes the method for the content of simultaneously measuring Chlorogenic Acid of Flos Lonicerae and galuteolin of the present invention, and adopts external standard method to calculate, and the results are shown in Table 1.
The traditional Chinese medicine honeysuckle Content of Chlorogenic Acid in 10 batches of different places of production of table 1 and the assay result of galuteolin
As shown in Table 1, utilize the method for simultaneously measuring Chlorogenic Acid of Flos Lonicerae and galuteolin content of the present invention, the content of the Chlorogenic Acid of Flos Lonicerae of surveying and galuteolin accurate, data are reliable.
As shown in Figure 1, from the UPLC-PDA chromatogram of sample 7 and reference substance, can find out, the crest of Chlorogenic Acid of Flos Lonicerae 1 and galuteolin 2 is clear, and good separating effect, is convenient to measure rapidly and accurately.
Embodiment 2
The method of the content of simultaneously measuring Chlorogenic Acid of Flos Lonicerae and galuteolin of the present invention, comprises the following steps:
(1) preparation of reference substance solution: precision takes chlorogenic acid, galuteolin reference substance: 0.889mg, 0.920mg taking acetonitrile-0.4% phosphate aqueous solution as solvent, is settled to 2mL, as storing solution in brown volumetric flask.The reference substance solution of other different quality concentration is obtained by storing solution dilution.
(2) preparation of need testing solution: the about 0.25g of extracting honeysuckle powder (crossing 60 mesh sieves), accurately weighed, be placed in tool plug conical flask, precision adds 70% ethanol water 30mL, weighed weight, ultrasonic extraction (power 250W, frequency 40HZ) 45min, let cool, supply the weight of minimizing with 70% ethanol water, shake up, filter, get subsequent filtrate appropriate, cross 0.22 μ m miillpore filter, to obtain final product.
(3) chromatographic condition: chromatographic column is Waters Acquity UPLC BEH RP
18(100mm × 2.1mm i.d., 1.7 μ are m); Room temperature, mobile phase is: 0.4% phosphoric acid water (A)-acetonitrile (B), gradient elution, equilibration time is 5min, gradient elution program: 0～2min, 5%～16%B; 2～4min, 16%～20%B; 4～8min, 20%～50%B; 8～10min, 50%～100%B; 10～11min, 100%～5%B; Sample size: 3 μ L, detect wavelength: 242nm, volumetric flow rate 0.2mL/min.
(4) foundation of typical curve
A, the investigation of linear relationship, detectability (LOD) and quantitative limit (LOQ)
Precision is drawn reference substance storing solution respectively, becomes the reference substance solution of a series of variable concentrations with mobile phase stepwise dilution, and sample introduction 3 μ L respectively, measure (n=3).Chlorogenic acid and galuteolin are taking concentration X as horizontal ordinate, and average peak area Y is that ordinate carries out linear regression, investigate linear relationship.Get mixing reference substance storing solution appropriate, add mobile phase stepwise dilution, under above-mentioned chromatographic condition, sample introduction is measured, and signal to noise ratio (S/N ratio) is detectability (LOD) and the quantitative limit (LOQ) that the concentration of 3: 1 and 10: 1 o'clock is respectively chlorogenic acid and galuteolin.As shown in Table 2, each regression equation is good linear relationship.
Typical curve, related coefficient, the range of linearity, LOD and the LOQ measurement result of table 2 chlorogenic acid and galuteolin
B, precision, repeatability and stability test
The accurate reference substance mixed liquor 3 μ L that draw, continuous sample introduction 6 times under above-mentioned chromatographic condition, the RSD value that records chlorogenic acid and galuteolin is respectively: 0.85%, 1.04%, result shows that the precision of instrument is good.Get with the about 0.25g of a collection of Honeysuckle Flower, totally 6 parts, accurately weighed, by test sample, preparation method makes need testing solution, under above-mentioned chromatographic condition, measures, and the average content that records chlorogenic acid is 34.86mg/g, and RSD is 1.17%; The average content of galuteolin is 1.24mg/g, and RSD is 1.28%, and result shows that this method repeatability is good.Get with a collection of Honeysuckle Flower (No: 7 Qufu City, Shandong Provinces) need testing solution, under room temperature, place, respectively at 0,2,4,8,16,24h sample introduction, 3 μ L, measure by above-mentioned chromatographic condition, record peak area, the RSD value of chlorogenic acid and galuteolin peak area is respectively 1.64%, 1.59%, and result shows that need testing solution is stable in 24h.
C, recovery test
Adopt application of sample absorption method.Get 9 parts of the Honeysuckle Flowers of known content, every part of about 0.125g, accurately weighed, be divided into 3 groups, by low (80%), in (100%), high (120%) concentration respectively precision adds a certain amount of chlorogenic acid and galuteolin reference substance solution, according to preparation method's preparation of test sample, measure calculate recovery rate at above-mentioned chromatographic condition.Result demonstration, the recovery reaches more than 97%, in table 3 entirely.Result confirms that the method for setting up has higher accuracy for the content of measuring Chlorogenic Acid of Flos Lonicerae and galuteolin simultaneously.
Table 3 application of sample recovery test result (n=9)
Although embodiment of the present invention are open as above, but it is not restricted to listed utilization in instructions and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend of describing.
Claims (5)
1. a method of simultaneously measuring Chlorogenic Acid of Flos Lonicerae and galuteolin content, is characterized in that, can carry out quantitative test to Chlorogenic Acid of Flos Lonicerae and galuteolin simultaneously, and it comprises the following steps:
Ultrasound wave extracts: extracting honeysuckle powder sieves, according to 1: 80-1: 120 solid-to-liquid ratio, add 70% ethanol water, weighed weight, ultrasonic extraction 45-60min, lets cool, and obtains Flos Lonicerae extractive solution;
Quantitative test: utilize UPLC-PDA method to carry out quantitative test, wherein UPLC condition: chromatographic column is Waters Acquity UPLC BEH RP
18(100mm × 2.1mm i.d., 1.7 μ are m); Room temperature, mobile phase: A:B=0.4% phosphoric acid water: acetonitrile; Condition of gradient elution: equilibration time is 5min; Gradient elution program: 0～2min, 5%～16%B; 2～4min, 16%～20%B; 4～8min, 20%～50%B; 8～10min, 50%～100%B; 10～11min, 100%～5%B; Sample size: 3 μ L, detect wavelength: 242nm, volumetric flow rate 0.2mL/min.
2. measure as claimed in claim 1 the method for Chlorogenic Acid of Flos Lonicerae and galuteolin content simultaneously, it is characterized in that, in ultrasound wave extraction step, Honeysuckle Flower was according to the solid-to-liquid ratio of 1: 120, add 70% ethanol water, weighed weight, ultrasonic extraction 45min, let cool, obtain Flos Lonicerae extractive solution.
3. measure as claimed in claim 1 the method for Chlorogenic Acid of Flos Lonicerae and galuteolin content simultaneously, it is characterized in that, in quantitative test step, also comprise: the preparation of reference substance solution: precision takes chlorogenic acid, galuteolin reference substance: 0.889mg, 0.920mg, taking acetonitrile-0.4% phosphate aqueous solution as solvent, in brown volumetric flask, be settled to 2mL, as storing solution.The reference substance solution of other different quality concentration is obtained by storing solution dilution.
4. the method for simultaneously measuring as claimed in claim 1 Chlorogenic Acid of Flos Lonicerae and galuteolin content, is characterized in that, in ultrasound wave extraction step, Honeysuckle Flower is crossed 65-80 mesh sieve in advance.
5. measure as claimed in claim 1 the method for Chlorogenic Acid of Flos Lonicerae and galuteolin content simultaneously, it is characterized in that, before quantitative test, carry out the preparation of need testing solution, comprise: extracting honeysuckle extract 70% ethanol water is supplied weight, shake up, filter, cross 0.22 μ m miillpore filter, obtain need testing solution.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104297374A (en) * | 2014-10-14 | 2015-01-21 | 南江县百草中药材有限公司 | Honeysuckle quality detection method |
| CN105486766A (en) * | 2015-10-13 | 2016-04-13 | 华南理工大学 | Method for determining content of three antioxidation active compositions in honeysuckle flower and plant thereof by employing HPLC |
| CN105911154A (en) * | 2016-02-01 | 2016-08-31 | 广西医科大学 | Method for determination of chlorogenic acid, galuteolin and total flavone content of honeysuckle |
| CN109633013A (en) * | 2018-12-29 | 2019-04-16 | 重庆医药高等专科学校 | A kind of honeysuckle multi objective quantitative analysis method |
| CN112147265A (en) * | 2020-09-17 | 2020-12-29 | 天津市农业质量标准与检测技术研究所 | Honeysuckle anti-inflammatory quality marker screening and quality identification method and application |
| CN112444572A (en) * | 2019-08-30 | 2021-03-05 | 大连民族大学 | Method for measuring luteolin content in pteris crassipes by HPLC-DAD |
| CN113552256A (en) * | 2021-07-20 | 2021-10-26 | 山东中医药大学 | Method for simultaneously determining content of 20 flavonoid components in honeysuckle |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007302751A (en) * | 2006-05-09 | 2007-11-22 | Musashino Chemical Laboratory Ltd | Fading inhibitor of natural pigment or food and drink comprising the same |
| CN103070450A (en) * | 2013-01-28 | 2013-05-01 | 山东省分析测试中心 | Storage and fresh preservation method of honeysuckle |
-
2014
- 2014-06-26 CN CN201410292908.4A patent/CN104020235B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007302751A (en) * | 2006-05-09 | 2007-11-22 | Musashino Chemical Laboratory Ltd | Fading inhibitor of natural pigment or food and drink comprising the same |
| CN103070450A (en) * | 2013-01-28 | 2013-05-01 | 山东省分析测试中心 | Storage and fresh preservation method of honeysuckle |
Non-Patent Citations (4)
| Title |
|---|
| 卢建峰: "超高压液相色谱法测定金银花中绿原酸的含量", 《安徽农业科学》, vol. 39, no. 7, 31 July 2011 (2011-07-31) * |
| 张元元等: "高效液相色谱法同时测定金银花中绿原酸和木樨草苷的含量", 《天津中医药大学学报》, vol. 30, no. 2, 30 June 2011 (2011-06-30) * |
| 施法等: "金银花中3种成分含量的超高速液相色谱法同时测定", 《时珍国医国药》, vol. 21, no. 11, 30 November 2010 (2010-11-30) * |
| 韩永成等: "不同产地金银花药材的 UPLC 指纹图谱分析", 《中国实验方剂学杂志》, vol. 20, no. 2, 31 January 2014 (2014-01-31), pages 67 - 69 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104297374A (en) * | 2014-10-14 | 2015-01-21 | 南江县百草中药材有限公司 | Honeysuckle quality detection method |
| CN105486766A (en) * | 2015-10-13 | 2016-04-13 | 华南理工大学 | Method for determining content of three antioxidation active compositions in honeysuckle flower and plant thereof by employing HPLC |
| CN105911154A (en) * | 2016-02-01 | 2016-08-31 | 广西医科大学 | Method for determination of chlorogenic acid, galuteolin and total flavone content of honeysuckle |
| CN109633013A (en) * | 2018-12-29 | 2019-04-16 | 重庆医药高等专科学校 | A kind of honeysuckle multi objective quantitative analysis method |
| CN112444572A (en) * | 2019-08-30 | 2021-03-05 | 大连民族大学 | Method for measuring luteolin content in pteris crassipes by HPLC-DAD |
| CN112147265A (en) * | 2020-09-17 | 2020-12-29 | 天津市农业质量标准与检测技术研究所 | Honeysuckle anti-inflammatory quality marker screening and quality identification method and application |
| CN113552256A (en) * | 2021-07-20 | 2021-10-26 | 山东中医药大学 | Method for simultaneously determining content of 20 flavonoid components in honeysuckle |
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| CN104020235B (en) | 2016-05-04 |
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