CN106047951A - Method for extracting cinnamyl aldehyde, coumarin and o-methoxycinnamaldehyde from cinnamon leaf - Google Patents
Method for extracting cinnamyl aldehyde, coumarin and o-methoxycinnamaldehyde from cinnamon leaf Download PDFInfo
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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Abstract
The invention discloses a method for extracting cinnamyl aldehyde, coumarin and o-methoxycinnamaldehyde from cinnamon leaf, and belongs to the field of research and development of natural perfume. The cinnamyl aldehyde, the coumarin and the o-methoxycinnamaldehyde are prepared by pseudomonas aeruginosa XJ26 fermentation liquor, cinnamon leaf powder is added to extract the cinnamyl aldehyde, the coumarin and the o-methoxycinnamaldehyde, and finally steps of extracting, distilling under reduced pressure and the like are carried out. The voluntarily screened pseudomonas aeruginosa fermentation liquor is used as a biological extraction agent to extract the cinnamyl aldehyde, the coumarin and the o-methoxycinnamaldehyde from the cinnamon leaf for the first time, bacterial strains are cultured simply, reaction conditions are mild, the method is environmentally friendly, the extraction rate of the coumarin and the o-methoxycinnamaldehyde in the cinnamon leaf extracted by the method is higher than that of the coumarin and the o-methoxycinnamaldehyde in the cinnamon leaf extracted by the conventional extracting method, and therefore, the method has good industrial application prospect.
Description
[technical field]
The invention belongs to natural perfume material research and development field, extract cinnamic aldehyde, coumarin particularly to one from basyleaves
And the method for o-methoxy cinnamic aldehyde.
[background technology]
Cortex Cinnamomi (Cinnamomum cassia) belong to canella, its branch and leaf, flower, really, skin all contain enrich Oleum Cinnamomi.
The Ramulus Cinnamomi leaf oil yield having document report spring and autumn is respectively 1.87%～2.06% and 0.98%～1.10% (see " economical
Woods is studied " .2006,24 (2): 9-13, Qin Yurong etc.).Oleum Cinnamomi is the volatile oil of a kind of complicated, has the most identified
Component has more than 50, is mainly composed of cinnamic aldehyde, and content accounts for 50%～95%.Additionally contain a small amount of O-methoxy Cortex Cinnamomi
Aldehyde, benzaldehyde, coumarin, cinnamyl acetate etc..At present, the method extracting Oleum Cinnamomi has steam distillation, organic solvent to extract
Method, supercritical CO2Extraction, molecularly distilled, ultrasonic extraction etc., the composition of Different Extraction Method gained Oleum Cinnamomi is the poorest
Different.There are some researches show common steam distillation obtain Oleum Cinnamomi be mainly composed of cinnamic aldehyde (84.22%), benzaldehyde
(1.1%), coumarin (3.82%), o-methoxy cinnamic aldehyde (7.07%)；And the main one-tenth of the Oleum Cinnamomi that water diffusion method obtains
It is divided into cinnamic aldehyde (87.89%), benzaldehyde (1.55%), cinnamyl acetate (3.92%) o-methoxy cinnamic aldehyde (4.56%)
(see " research of water diffusion distillation extraction basyleaves effective ingredient ". Guangxi: Guangxi University, 2014, defend to the south).Scholar is also had to visit
Study carefully and utilized steam distillation to extract the process conditions of cassia oil component in Laurus nobilis, obtained cinnamic aldehyde and o-methoxy cinnamic aldehyde
Extraction ratio is respectively 2.08% and 0.106% (see " fine chemistry industry " 2014,31 (1): 50-53, Zhang Yujiao etc.).Additionally, with stone
Oil ether, normal hexane, the chemical composition of 3 kinds of solvent extraction Cortex cinnamomi japonici (Ramulus Cinnamomi) quintessence oils of chloroform present not in kind and percentage contents
All without o-methoxy cinnamic aldehyde, coumarin and acetic acid Cortex Cinnamomi in the most significant difference, and the component that content is more than 0.1%
Ester (see " China's agronomy circular " 2012,28 (9): 264-269, Zhang Yan etc.).
The present inventor chances in scientific experiments, with the fermentation liquid of a kind of microorganism that obtains from nature for extracting
Agent, is significantly higher than traditional extraction method to the extraction ratio of coumarin in basyleaves and o-methoxy cinnamic aldehyde.Identified: this microorganism
For Pseudomonas aeruginosa Pseudomonas aeruginosa, and named Pseudomonas aeruginosa XJ26.In
It is that the Pseudomonas aeruginosa Pseudomonas aeruginosaXJ26 of oneself screening purification is used for extracting Cortex Cinnamomi folic acid by we
The research of oil, it is determined that extract cinnamic aldehyde, coumarin and the production work of o-methoxy cinnamic aldehyde in basyleaves for extractant with it
Skill.
[summary of the invention]
The technical problem to be solved in the present invention is to provide a kind of from basyleaves extraction cinnamic aldehyde, coumarin and O-methoxy meat
The method of cinnamic aldehyde, to solve the problems such as the extraction ratio of coumarin and o-methoxy cinnamic aldehyde is low.The present invention utilizes first and sieves voluntarily
The P. aeruginosa fermented liquid of choosing extracts the cinnamic aldehyde of basyleaves, coumarin and O-methoxy Cortex Cinnamomi as biological extraction agent
Aldehyde, strain culturing is simple, and reaction condition is gentle, and environmental friendliness has good industrial applications prospect.
In order to solve above technical problem, the present invention by the following technical solutions:
A kind of from basyleaves extraction cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde, comprise the following steps:
S1: Pseudomonas aeruginosa XJ26 is accessed culture fluid, described culture fluid includes following component: carbon source, nitrogen source, phosphoric acid
Hydrogen dipotassium, sodium chloride, bitter salt, green vitriol, be 30 DEG C in temperature, and rotating speed is to cultivate under 180r/min
72h, prepares Pseudomonas aeruginosa XJ26 fermentation liquid；
The pH value of S2: the regulating step S1 Pseudomonas aeruginosa XJ26 fermentation liquid prepared, adds cassia leaf powder, at rotating speed is
Mechanical shaking extraction under 180r/min, prepares bacterium extract；
S3: bacterium extract step S2 prepared is centrifugal 30min under rotating speed is 4000r/min, takes supernatant, adds extraction
Agent carries out extracting 1～2 time, prepares containing cinnamic aldehyde, coumarin and o-methoxy cinnamic aldehyde after decompression Distillation recovery extractant
Muddy grease.
Further, culture fluid described in step S1 includes following component: carbon source 10～50g/L, nitrogen source 10～30g/L,
Dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/L, green vitriol 0.01g/L.
Further, described carbon source is glucose.
Further, described nitrogen source is peptone.
Further, pH value described in step S2 is 5～7.
Further, described pH value uses hydrochloric acid or sodium hydroxide solution to be adjusted.
Further, cassia leaf powder described in step S2 is to cross 60 mesh sieve gained after basyleaves are pulverized.
Further, Extracting temperature described in step S2 is 26～34 DEG C
Further, the time used under described Extracting temperature is 48～72h.
Further, extractant described in step S3 is ethyl acetate.
The method have the advantages that
The present invention utilizes first and extracts basyleaves from the P. aeruginosa fermented liquid of row filter as biological extraction agent
Cinnamic aldehyde, coumarin and o-methoxy cinnamic aldehyde, strain culturing is simple, and reaction condition is gentle, environmental friendliness, uses the present invention's
Method is higher than traditional extraction method to the extraction ratio of coumarin in basyleaves and o-methoxy cinnamic aldehyde, and having good industrialization should
Use prospect.
[detailed description of the invention]
For ease of being more fully understood that the present invention, being illustrated by following example, these embodiments belong to the present invention's
Protection domain, but it is not intended to protection scope of the present invention.
In an embodiment, described extract cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde from basyleaves, including with
Lower step:
S1: Pseudomonas aeruginosa XJ26 is accessed culture fluid, described culture fluid includes following component: glucose 10～50g/
L, peptone 10～30g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/L, seven hydrated sulfuric acid are sub-
Ferrum 0.01g/L, is 30 DEG C in temperature, and rotating speed is cultivation 72h under 180r/min, prepares Pseudomonas aeruginosa XJ26 fermentation liquid；
The pH value of S2: the regulating step S1 Pseudomonas aeruginosa XJ26 fermentation liquid prepared is 5～7, and described pH value uses
2mol/L hydrochloric acid or 2mol/L sodium hydroxide solution are adjusted, and add 60 mesh cassia leaf powders, are 26～34 DEG C at Extracting temperature,
Vibration rotating speed is under 180r/min, extracts 48～72h, prepares bacterium extract；
S3: bacterium extract step S2 prepared is centrifugal 30min under rotating speed is 4000r/min, takes supernatant, adds acetic acid
Ethyl ester carries out extracting 1～2 time, and described clear liquid is 1:1 with the volume ratio of extractant, prepares and contain after decompression Distillation recovery extractant
The muddy grease of cinnamic aldehyde, coumarin and o-methoxy cinnamic aldehyde.
Below by more specifically embodiment, the present invention will be described.
Pseudomonas aeruginosa (Pseudomonas aeruginosa) XJ26 of the present invention is that one belongs to antibacterial class vacation list
The bacterial strain of born of the same parents Pseudomonas, this bacterial strain is to separate from soil, and successively through cinnamic aldehyde tolerance screening and degraded basyleaves ability
Screening multi-turns screen obtain, according to 16SrDNA molecular biology identification, this bacterium and Pseudomonas aeruginosa
The homology of (Pseudomonas aeruginosa) is 99%.
Embodiment 1
With Pseudomonas aeruginosa (Pseudomonas aeruginosa) the XJ26 thalline on inoculating loop picking inclined-plane to training
(glucose 10g/L, peptone 10g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/ in nutrient solution
L, green vitriol 0.01g/L.Sterilizing 25min at 121 DEG C).At 30 DEG C, constant temperature culture oscillator (180r/min) is cultivated
72h, obtains Pseudomonas aeruginosa XJ26 fermentation liquid.In fermentation liquid, add the 10g/L cassia leaf powder of 60 mesh, shake in constant temperature culture
Swing in device 30 DEG C, 180r/min extract 72h, obtain bacterium extract.
Embodiment 2
With Pseudomonas aeruginosa (Pseudomonas aeruginosa) the XJ26 thalline on inoculating loop picking inclined-plane to training
(glucose 30g/L, peptone 20g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/ in nutrient solution
L, green vitriol 0.01g/L.Sterilizing 25min at 121 DEG C).At 30 DEG C, constant temperature culture oscillator (180r/min) is cultivated
72h, obtains P. aeruginosa fermented liquid.The 10g/L cassia leaf powder of 60 mesh is added, in constant temperature culture oscillator in fermentation liquid
In 30 DEG C, 180r/min extract 72h, obtain bacterium extract.
Embodiment 3
With Pseudomonas aeruginosa (Pseudomonas aeruginosa) the XJ26 thalline on inoculating loop picking inclined-plane to training
(glucose 50g/L, peptone 30g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/ in nutrient solution
L, green vitriol 0.01g/L.Sterilizing 25min at 121 DEG C).At 30 DEG C, constant temperature culture oscillator (180r/min) is cultivated
72h, obtains P. aeruginosa fermented liquid.The 10g/L cassia leaf powder of 60 mesh is added, in constant temperature culture oscillator in fermentation liquid
In 30 DEG C, 180r/min extract 72h, obtain bacterium extract.
Embodiment 4
With Pseudomonas aeruginosa (Pseudomonas aeruginosa) the XJ26 thalline on inoculating loop picking inclined-plane to training
(glucose 30g/L, peptone 20g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/ in nutrient solution
L, green vitriol 0.01g/L.Sterilizing 25min at 121 DEG C).At 30 DEG C, constant temperature culture oscillator (180r/min) is cultivated
72h, obtains P. aeruginosa fermented liquid.With the pH value of the fermentation liquid of 2mol/L hydrochloric acid regulation fresh cultured to 5, add 60 mesh
10g/L cassia leaf powder, in constant temperature culture oscillator 30 DEG C, 180r/min extract 72h, obtain bacterium extract.
Embodiment 5
With Pseudomonas aeruginosa (Pseudomonas aeruginosa) the XJ26 thalline on inoculating loop picking inclined-plane to training
(glucose 30g/L, peptone 20g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/ in nutrient solution
L, green vitriol 0.01g/L.Sterilizing 25min at 121 DEG C).At 30 DEG C, constant temperature culture oscillator (180r/min) is cultivated
72h, obtains P. aeruginosa fermented liquid.With the pH value of the fermentation liquid of 2mol/L hydrochloric acid regulation fresh cultured to 6, add 60 mesh
10g/L cassia leaf powder, in constant temperature culture oscillator 30 DEG C, 180r/min extract 72h, obtain bacterium extract.
Embodiment 6
With Pseudomonas aeruginosa (Pseudomonas aeruginosa) the XJ26 thalline on inoculating loop picking inclined-plane to training
(glucose 30g/L, peptone 20g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/ in nutrient solution
L, green vitriol 0.01g/L.Sterilizing 25min at 121 DEG C).At 30 DEG C, constant temperature culture oscillator (180r/min) is cultivated
72h, obtains P. aeruginosa fermented liquid.With the pH value of the fermentation liquid of 2mol/L sodium hydroxide solution regulation fresh cultured to 7,
Add the 10g/L cassia leaf powder of 60 mesh, in constant temperature culture oscillator 30 DEG C, 180r/min extract 72h, obtain bacterium extract.
Embodiment 7
With Pseudomonas aeruginosa (Pseudomonas aeruginosa) the XJ26 thalline on inoculating loop picking inclined-plane to training
(glucose 30g/L, peptone 20g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/ in nutrient solution
L, green vitriol 0.01g/L.Sterilizing 25min at 121 DEG C).At 30 DEG C, constant temperature culture oscillator (180r/min) is cultivated
72h, obtains P. aeruginosa fermented liquid.With the pH value of the fermentation liquid of 2mol/L hydrochloric acid regulation fresh cultured to 6, add 60 mesh
10g/L cassia leaf powder, in constant temperature culture oscillator 26 DEG C, 180r/min extract 72h, obtain bacterium extract.
Embodiment 8
With Pseudomonas aeruginosa (Pseudomonas aeruginosa) the XJ26 thalline on inoculating loop picking inclined-plane to training
(glucose 30g/L, peptone 20g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/ in nutrient solution
L, green vitriol 0.01g/L.Sterilizing 25min at 121 DEG C).At 30 DEG C, constant temperature culture oscillator (180r/min) is cultivated
72h, obtains P. aeruginosa fermented liquid.With the pH value of the fermentation liquid of 2mol/L hydrochloric acid regulation fresh cultured to 6, add 60 mesh
10g/L cassia leaf powder, in constant temperature culture oscillator 28 DEG C, 180r/min extract 72h, obtain bacterium extract.
Embodiment 9
With Pseudomonas aeruginosa (Pseudomonas aeruginosa) the XJ26 thalline on inoculating loop picking inclined-plane to training
(glucose 30g/L, peptone 20g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/ in nutrient solution
L, green vitriol 0.01g/L.Sterilizing 25min at 121 DEG C).At 30 DEG C, constant temperature culture oscillator (180r/min) is cultivated
72h, obtains P. aeruginosa fermented liquid.With the pH value of the fermentation liquid of 2mol/L hydrochloric acid regulation fresh cultured to 6, add 60 mesh
10g/L cassia leaf powder, in constant temperature culture oscillator 34 DEG C, 180r/min extract 72h, obtain bacterium extract.
Embodiment 10
With Pseudomonas aeruginosa (Pseudomonas aeruginosa) the XJ26 thalline on inoculating loop picking inclined-plane to training
(glucose 30g/L, peptone 20g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/ in nutrient solution
L, green vitriol 0.01g/L.Sterilizing 25min at 121 DEG C).At 30 DEG C, constant temperature culture oscillator (180r/min) is cultivated
72h, obtains P. aeruginosa fermented liquid.With the pH value of the fermentation liquid of 2mol/L hydrochloric acid regulation fresh cultured to 6, add 60 mesh
10g/L cassia leaf powder, in constant temperature culture oscillator 28 DEG C, 180r/min extract 48h, obtain bacterium extract.
Embodiment 11
With Pseudomonas aeruginosa (Pseudomonas aeruginosa) the XJ26 thalline on inoculating loop picking inclined-plane to training
(glucose 30g/L, peptone 20g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride 0.5g/L, bitter salt 0.5g/ in nutrient solution
L, green vitriol 0.01g/L.Sterilizing 25min at 121 DEG C).At 30 DEG C, constant temperature culture oscillator (180r/min) is cultivated
72h, obtains P. aeruginosa fermented liquid.With the pH value of the fermentation liquid of 2mol/L hydrochloric acid regulation fresh cultured to 6, add 10g/
L cassia leaf powder, in constant temperature culture oscillator 28 DEG C, 180r/min extract 60h, obtain bacterium extract.
The bacterium extract of Example 1-11 centrifugal 30min under rotating speed is 4000r/min, takes the bodies such as supernatant, use respectively
Long-pending ethyl acetate extracts 1 time, prepares containing cinnamic aldehyde, coumarin and the muddiness of o-methoxy cinnamic aldehyde after decompression distillation to 1～2mL
Grease, described muddy grease ethyl acetate constant volume is in 5mL volumetric flask.Take 0.4 μ L GC and carry out quantitative analysis, and use
GC-MS carries out qualitative analysis, and analysis condition is as follows:
Chromatographic condition: chromatographic column: Rxi-5Sil (30m × 0.25mm × 0.25 μm)；Injector temperature: 250 DEG C；Program liter
Temperature process: post initial temperature 100 DEG C, retains 1min, rises to 200 DEG C with 5 DEG C/min, retains 1min, then rises to 250 DEG C with 8 DEG C/min；
Split ratio 1:30.
Mass Spectrometry Conditions: electron bombardment (EI) ion source, ionizing energy 70eV, electron multiplier voltage 1.5kV, full scan side
Formula.
Qualitative and qualitative analysis: according to total ion current figure, application NIST08, NIST08s mass spectral database retrieval, it is thus identified that extract
Primary product be cinnamic aldehyde, coumarin and o-methoxy cinnamic aldehyde.Use external standard method, prepare the Cortex Cinnamomi of a series of variable concentrations
Aldehyde, coumarin and o-methoxy cinnamic aldehyde standard solution carry out GC chromatography.With the peak area of each concentration standard solution to it
Mass concentration (μ g/mL) carries out linear regression, obtains regression equation, calculates cinnamic aldehyde, coumarin and o-methoxy cinnamic aldehyde
Extraction ratio is shown in Table 1.
Cinnamic aldehyde, coumarin and the extraction ratio of o-methoxy cinnamic aldehyde in table 1 embodiment 1-11
From table 1, the method using the present invention is high to the extraction ratio of coumarin in basyleaves and o-methoxy cinnamic aldehyde
In traditional extraction method.
Above content it cannot be assumed that the present invention be embodied as be confined to these explanation, technology belonging to the present invention is led
For the those of ordinary skill in territory, without departing from the inventive concept of the premise, it is also possible to make some simple deduction or replace,
All should be considered as belonging to the scope of patent protection that the present invention is determined by the claims submitted to.
Claims (10)
1. one kind is extracted cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde from basyleaves, it is characterised in that include following
Step:
S1: Pseudomonas aeruginosa XJ26 is accessed culture fluid, described culture fluid includes following component: carbon source, nitrogen source, phosphoric acid hydrogen two
Potassium, sodium chloride, bitter salt, green vitriol, be 30 DEG C in temperature, and rotating speed is cultivation 72h under 180r/min, system
Obtain Pseudomonas aeruginosa XJ26 fermentation liquid；
The pH value of S2: the regulating step S1 Pseudomonas aeruginosa XJ26 fermentation liquid prepared, adds cassia leaf powder, at rotating speed is
Mechanical shaking extraction under 180r/min, prepares bacterium extract；
S3: bacterium extract step S2 prepared is centrifugal 30min under rotating speed is 4000r/min, takes supernatant, adds extractant and enters
Row extraction 1～2 time, prepares containing cinnamic aldehyde, coumarin and the muddiness of o-methoxy cinnamic aldehyde after decompression Distillation recovery extractant
Grease.
Cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde, its feature is extracted the most according to claim 1 from basyleaves
Being, culture fluid described in step S1 includes following component: carbon source 10～50g/L, nitrogen source 10～30g/L, dipotassium hydrogen phosphate 1g/
L, sodium chloride 0.5g/L, bitter salt 0.5g/L, green vitriol 0.01g/L.
Cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde, its feature is extracted the most according to claim 2 from basyleaves
Being, described carbon source is glucose.
Cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde, its feature is extracted the most according to claim 2 from basyleaves
Being, described nitrogen source is peptone.
Cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde, its feature is extracted the most according to claim 1 from basyleaves
Being, pH value described in step S2 is 5～7.
Cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde, its feature is extracted the most according to claim 5 from basyleaves
Being, described pH value uses hydrochloric acid or sodium hydroxide solution to be adjusted.
Cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde, its feature is extracted the most according to claim 1 from basyleaves
Being, cassia leaf powder described in step S2 is to cross 60 mesh sieve gained after basyleaves are pulverized.
Cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde, its feature is extracted the most according to claim 1 from basyleaves
Being, Extracting temperature described in step S2 is 26～34 DEG C.
Cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde, its feature is extracted the most according to claim 8 from basyleaves
Being, the time used under described Extracting temperature is 48～72h.
The most according to claim 1, extract cinnamic aldehyde, coumarin and the method for o-methoxy cinnamic aldehyde from basyleaves, it is special
Levying and be, extractant described in step S3 is ethyl acetate.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107151681A (en) * | 2017-05-31 | 2017-09-12 | 广西中医药大学 | A kind of method that Mucor indicus reduction cinnamic acid prepares natural 3 phenylpropanol |
| CN111013190A (en) * | 2020-01-14 | 2020-04-17 | 三益创价生物科技(深圳)有限公司 | Method and device for extracting composition rich in cinnamaldehyde from cinnamon leaves |
| CN111228851A (en) * | 2020-01-14 | 2020-06-05 | 三益创价生物科技(深圳)有限公司 | Method and device for extracting composition rich in cinnamaldehyde from cinnamon bark |
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| CN104498540A (en) * | 2014-12-03 | 2015-04-08 | 北京林业大学 | Method for producing 4-hydroxycinnamaldehyde by catalyzing recombinant strain and whole cells thereof |
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| CN104498540A (en) * | 2014-12-03 | 2015-04-08 | 北京林业大学 | Method for producing 4-hydroxycinnamaldehyde by catalyzing recombinant strain and whole cells thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107151681A (en) * | 2017-05-31 | 2017-09-12 | 广西中医药大学 | A kind of method that Mucor indicus reduction cinnamic acid prepares natural 3 phenylpropanol |
| CN111013190A (en) * | 2020-01-14 | 2020-04-17 | 三益创价生物科技(深圳)有限公司 | Method and device for extracting composition rich in cinnamaldehyde from cinnamon leaves |
| CN111228851A (en) * | 2020-01-14 | 2020-06-05 | 三益创价生物科技(深圳)有限公司 | Method and device for extracting composition rich in cinnamaldehyde from cinnamon bark |
| CN111228851B (en) * | 2020-01-14 | 2021-03-09 | 三益创价生物科技(深圳)有限公司 | Method and device for extracting composition rich in cinnamaldehyde from cinnamon bark |
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Application publication date: 20161026 |