CN105199852A - Process using Chongqing Fengjie Yunyan 87 flower buds as raw materials to extract essential oil and application of essential oil - Google Patents
Process using Chongqing Fengjie Yunyan 87 flower buds as raw materials to extract essential oil and application of essential oil Download PDFInfo
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Abstract
The invention discloses a process using Chongqing Fengjie Yunyan 87 flower buds as raw materials to extract essential oil and application of the essential oil. The process includes: adding distilled water into smashed Chongqing Fengjie Yunyan 87 flower buds according to the material-liquid ratio of 1:10-1:30, adding cellulase accounting for 2-10% of the weight of the tobacco flower buds, adding sodium chloride accounting for 5-25% of total weight, performing ultrasonic treatment for 10-40 minutes, and distilling with steam for 2-6 hours to prepare the flower bud essential oil. The flower bud essential oil prepared by the process has the advantages that the essential oil is capable of well eliminating OH free radicals and O2- free radicals and is a good antioxidant substance; meanwhile, the essential oil has certain antibacterial activity against escherichia coli, bacillus subtilis and paenibacillus polymyxa and is a good antibacterial agent. The Chongqing Fengjie Yunyan 87 flower bud essential oil prepared by the process can be used as an antioxidant agent and the antibacterial agent.
Description
Technical field
The invention belongs to tobacco leaf product technical field, be specifically related to a kind of using Chongqing Fengjie cloud and mist 87 bud for raw material extracts the technique of essential oil and the application as antioxidant and fungistat thereof
Background technology
For plants essential oil, research both domestic and external is too numerous to enumerate.Zhao Ling Yu have studied the volatile oil in extraction by steam distillation orange peel, finds that the method not only decreases energy consumption, and have install simple to operate, save the advantage such as reagent, decreasing pollution.Wang Hong etc. utilize CO
2extract Fructus Xanthii oil as extraction agent, and compare with the material that steam distillation extracts and find that the method can not replace steam distillation completely.Vian etc. utilize using microwave assisted aqueous extraction to spread gravity method and extract Peppermint essential oil.The method has fast, efficient, safe, energy-conservation advantage.Chen Liyan etc. find that various plants essential oil all has antibacterial, germicidal action when studying plants essential oil anti-microbial activity, are all widely used in clinical medicine.
Tobacco is a kind of disputable, but has the farm crop of great economic benefit, and be also plant the most non-food crop in the world, tobacco planting and Product processing play an important role in the national economic development.China introduces a fine variety tobacco very early, and output is quite large.Tobacco waste refers to that tobacco prophyll is being gathered and in the tobacco product course of processing, fail the tobacco integral part (cigarette comprises expanded cut tobacco and tobacco sheet) be applied in cigarette, comprise tobacco rod, tobacco plant upper tobacco leaf, fireworks, cigarette kind, vein, axillalry bud discarded in agriculture production, offal discarded in redrying and the tobacco leaf that cannot carry out outside the inferior grade of Cigarette processing or grade, offal, cigarette root, root sucker etc. that leaf roll enterprise accumulates.Up to the present the cultivated area of China tobacco and output all occupy first place in the world, and also mean that China can produce the tobacco waste of few hundred thousand tonnes of every year, these wastes not only contaminate environment, especially to the huge waste of resource.
Large quantifier elimination is had abroad to the field such as genetically engineered, physiologically active, biological activity of tobacco bud, LiuBF etc. study capillary electrophoresis chromatogram to the separation condition of tobacco flowering plant hormone, result shows, at optimum conditions, be separated seven Plant Hormones such as Plant hormones regulators,gibberellins, dormin, indolylacetic acid completely and only need 30min.Domestic aspect, tobacco bud is used for extracting balm through alcohol, distilled water extraction by Kang Wucheng etc., has formed " three cream " products such as burley bud sweet paste, flue-cured tobacco bud cream and Bailei tobacco extract at present, and has applied in perfuming cigarette.Permitted spring equality and adopted supercritical CO
2volatile component in extraction flue-cured tobacco bud, and composition Study is carried out to it, result shows, wherein containing 11 kinds of alcohols, 7 kinds of ester classes, 8 kinds of acids etc., in addition, are investigated the anti-oxidant activity of burley tobaccos bud polysaccharide.
In recent years, compared with China at present for the research of tobacco leaf, the research of tobacco waste mainly concentrates on the extraction of active substance in tobacco waste, manufacture the aspects such as tobacco sheet, expanded cut stem and papermaking, such as extraction nicotine, Salanesol from useless secondary tobacco leaf and offal, extract pectin from tobacco rod and offal, protein is extracted from fresh tobacco rod, adopt paper process again to be processed by material discarded in whole production of cigarettes process, laminate, and in Ying Yu production of cigarettes etc.In recent years along with the increase of production cost, the increase of environmental stress, and the develop rapidly of the subject such as biological chemistry, analytical chemistry, phytology, medical science and cross development, people have had more and more deep understanding to the extraction and isolation of essential oil, composition and biological activity etc., people recognize that essential oil is as a kind of natural plant prod gradually, and it has a series of physiological functions such as anti-oxidant, anti-ageing, antibacterial.
Tobacco bud essential oil as a kind of extract from tobacco waste a kind of natural biologically active substance, have exploitation potential quality, its application and DEVELOPMENT PROSPECT boundless.Strengthen studying the comprehensive development and utilization of mankind aspect favourable in tobacco waste, pay attention to, on the impact of environment, maximizing favourable factors and minimizing unfavourable ones simultaneously, allow tobacco give full play to contribution to the mankind.To the bioactive research of tobacco bud essential oil, develop a kind of novel tobacco end product, the comprehensive utilization for tobacco provides a new approach, and expands the range of application of tobacco.At present, not yet there are research to tobacco bud essential oil and relevant report in China.
Summary of the invention
The object of the present invention is to provide a kind of using Chongqing Fengjie cloud and mist 87 bud for raw material extracts the technique of essential oil and the application as antioxidant and fungistat thereof.
For achieving the above object, the present invention adopts following technical scheme
With the technique of Chongqing Fengjie cloud and mist 87 bud for raw material extraction essential oil, it is obtained by following step:
After Chongqing Fengjie cloud and mist 87 bud after pulverizing is added distilled water by solid-liquid ratio 1:10 ~ 1:30, cellulase is added by 2% ~ 10% of tobacco bud quality, after adding sodium-chlor by 5% ~ 25% of total mass, supersound process 10 ~ 40min, then adopts wet distillation 2-6h to prepare bud essential oil.Steam distillation is prepared essential oil and is adopted this area routine techniques.
The preferred technical scheme of extraction process of the present invention is: solid-liquid ratio is 1:15 ~ 1:25, and 5% ~ 7% adds cellulase, and 10% ~ 14% adds sodium-chlor, supersound process 10 ~ 25min, wet distillation 4-5h.
The most preferred technical scheme of extraction process of the present invention is: solid-liquid ratio is 1:20, and 6% adds cellulase, and 10% adds sodium-chlor, supersound process 15min, wet distillation 4h.Open separator lower end piston after cooling, moisture is slowly flowed out.Collect oil reservoir with liquid-transfering gun, and with dichloromethane rinse extractor three times, washing fluid and oil reservoir are merged, add anhydrous sodium sulphate and spend the night, after removing moisture, low-temperature rotary evaporates, and removing methylene dichloride obtains tobacco bud essential oil.
Invention also provides a kind of is the tobacco bud essential oil that raw material extracts the technique of essential oil and prepares with Chongqing Fengjie cloud and mist 87 bud.
Invention also provides the application of described tobacco bud essential oil as antioxidant, fungistat.
The application adopts ultrasonic enzymolysis process to extract Chongqing Fengjie cloud and mist 87 tobacco bud essential oil, and passing through the single factor experiment of five levels, research sodium chloride concentration A, cellulase addition B, solid-liquid ratio C, ultrasonic time D tetra-factors are on the impact of bud extraction of essential oil rate.Then Orthogonal Method is adopted to be optimized test design, show that ultrasonic enzymolysis process extracts the optimal extract process of tobacco bud essential oil, for sodium-chlor addition is 10%, cellulase addition 6%, solid-liquid ratio 1:20, ultrasonic time 15min, and under optimum process condition, the yield of Chongqing Fengjie tobacco bud essential oil is 0.566% (g/g).In addition, adopt o-phenanthroline, remove the anti-oxidant activity that the method for ultra-oxygen anion free radical have detected Chongqing Fengjie cloud and mist 87 bud essential oil, namely it is to OH free radical and O
2-the Scavenging activity of free radical.Filter paper enzyme and micro-dilution method is adopted to have detected the bacteriostasis property of Chongqing Fengjie cloud and mist 87 bud essential oil.
Compared to the prior art, beneficial effect of the present invention:
1) Economic and Efficiency Analysis: in recent years, due to develop rapidly and the cross development of the subjects such as biological chemistry, analytical chemistry, phytology, medical science, people have had more and more deep understanding to the extraction and isolation of essential oil, composition and biological activity etc., tobacco bud essential oil is as a kind of biologically active substance extracted in tobacco waste, there is certain physiological action, and because its raw material prepared is the tobacco bud discarded completely, resource is comparatively abundant and cheap, its application and DEVELOPMENT PROSPECT boundless.The preparation of high purity bud essential oil under lab can realize, by the optimal extraction technology of research Chongqing Fengjie Yunnan 87 bud essential oil, and analyze the oil composition of bud essence, anti-oxidant, antibacterial character, develop a kind of novel tobacco related products, comprehensive utilization for tobacco provides a new approach, improve tobacco waste utility value, and expand the range of application of tobacco.In addition, because preparation technology's flow process of tobacco bud essential oil is simple, with low cost, can be applicable in large-scale industrial production, therefore, the exploitation of tobacco bud essential oil end product has considerable economic benefit.
2) social benefit analysis: at present, countries in the world fully utilize tobacco, especially discard the research and development of nothing for tobacco in the ascendant, and also constantly deeply, compared with countries in Europe, the research of China in this field comparatively backwardness.It is appreciated that China is tobacco leaf production big country and the consumption big country of tobacco, the annual tobacco waste producing few hundred thousand tonnes of, is not only the waste to resource, also can produces very large environmental pollution.Therefore, along with going deep into of tobacco waste comprehensive utilizating research, and the exploitation of a series of high value added product, for reduction tobacco leaf production cost, increase economic efficiency, reduce environmental pollution, alleviate environmental protection pressure etc. significant.Tobacco bud essential oil is a kind of natural essential oil product extracted from discarded tobacco bud, and it has the physiological functions such as anti-oxidant, anti-ageing, antibacterial.We can set about from the extraction of the utilization to tobacco refuse, tobacco bud essential oil, bioactivity research and applied research, in conjunction with the development of new and high technology, tobacco comprehensive exploitation with utilize field, open the situation that breaks.
Accompanying drawing explanation
Fig. 1 is the ultrasonic enzymolysis process extraction process route map of Chongqing Fengjie cloud and mist 87 tobacco bud essential oil;
Fig. 2 be Chongqing Fengjie cloud and mist 87 tobacco bud essential oil to the Scavenging activity of OH free radical, essential oil of tobaccos (●) BHT (zero);
Fig. 3 is that Chongqing Fengjie cloud and mist 87 tobacco bud essential oil is to O
2-the Scavenging activity of free radical, essential oil of tobaccos (●) BHT (zero);
Embodiment
Be that the present invention is described further by the following examples, but protection scope of the present invention is not limited thereto.
Embodiment 1
A kind of with the method for Chongqing Fengjie cloud and mist 87 bud for raw material extraction essential oil, it is obtained by following step: after Chongqing Fengjie cloud and mist 87 bud after pulverizing is added distilled water by solid-liquid ratio 1:15 ~ 1:25, cellulase is added by 5% ~ 7% of tobacco bud quality, after adding sodium-chlor by 10% ~ 14% of total mass, supersound process 10 ~ 25min, then adopt wet distillation 4h, open separator lower end piston after cooling, moisture is slowly flowed out.Collect oil reservoir with liquid-transfering gun, and with dichloromethane rinse extractor three times, washing fluid and oil reservoir are merged, add anhydrous sodium sulphate and spend the night, after removing moisture, low-temperature rotary evaporates, and removing methylene dichloride obtains tobacco bud essential oil.
By the single factor experiment of five levels, research sodium chloride concentration A, cellulase addition B, solid-liquid ratio C, ultrasonic time D tetra-factors are on the impact of bud extraction of essential oil rate.Then Orthogonal Method is adopted to be optimized test design.
Sodium-chlor addition: at solid-liquid ratio 1:20, cellulase addition 4% (enzyme quality: bud quality), ultrasonic time 15min, sodium-chlor addition (sodium-chlor quality: distilled water volume) 6%, 8%, 10%, 12%, test under the condition of 14%, draw optimum level, select 8%, 10%, 12% 3 level carries out orthogonal test.
Cellulase addition: at solid-liquid ratio 1:20, sodium-chlor addition 10% (sodium-chlor quality: distilled water volume), ultrasonic time 15min, cellulase addition (enzyme quality: bud quality) is respectively 1%, 2%, 4%, 6%, test under the condition of 8%, draw Best Times level, select 4%, 6% and 8% 3 level design orthogonal test.
Solid-liquid ratio: at cellulase addition 4% (enzyme quality: bud quality), sodium-chlor addition 10% (sodium-chlor quality: distilled water volume), ultrasonic time 15min, solid-liquid ratio is 1:10 respectively, 1:15,1:20,1:25, test under the condition of 1:30W, draw best solid-liquid ratio.1:20,1:25,1:30 tri-levels are selected to carry out orthogonal experimental design.
Ultrasonic time: at solid-liquid ratio 1:20, cellulase addition 4% (enzyme quality: bud quality), sodium-chlor addition 10% (sodium-chlor quality: distilled water volume), ultrasonic time 5,10,15,20, test under the condition of 25min, draw optimum level, select 10,15,20min, tri-levels carry out orthogonal test.
Orthogonal test: on the basis that experiment of single factor is analyzed, have selected 1:20, 1:25, 1:30 tri-liquid ratio levels, 4%, 6% and 8% 3 cellulase addition (enzyme quality: bud quality) level, 8%, 10%, 12% 3 sodium-chlor addition (sodium-chlor quality: distilled water volume) level, 10, 15, 20min tri-ultrasonic time levels, carry out orthogonal experimental design, the optimal extract process that the ultrasonic enzymolysis process that can draw extracts tobacco bud essential oil is: sodium-chlor addition (sodium-chlor quality: distilled water volume) is 10%, cellulase addition 6%, solid-liquid ratio 1:20, ultrasonic time 15min, and under optimum process condition, the yield of Chongqing Fengjie tobacco bud essential oil is 0.566% (g/g).The results are shown in Table 1.
The ultrasonic enzymolysis process of table 1 extracts tobacco bud essential oil orthogonal experiments table
Embodiment 2
With the method for Chongqing Fengjie cloud and mist 87 bud for raw material extraction essential oil, it is obtained by following step:
After Chongqing Fengjie cloud and mist 87 bud after pulverizing is added distilled water by solid-liquid ratio 1:20, cellulase is added by 6% of tobacco bud quality, after adding sodium-chlor by 10% of total mass, supersound process 15min, then wet distillation 4h is adopted, open separator lower end piston after cooling, moisture is slowly flowed out.Collect oil reservoir with liquid-transfering gun, and with dichloromethane rinse extractor three times, washing fluid and oil reservoir are merged, add anhydrous sodium sulphate and spend the night, after removing moisture, low-temperature rotary evaporates, and removing methylene dichloride obtains tobacco bud essential oil.Embodiment 3
Application test: the anti-oxidant research of Chongqing Fengjie cloud and mist 87 tobacco bud
1) tobacco bud essential oil is to the mensuration of OH free radical scavenging activity:
Dehydrated alcohol with 50% respectively configuration concentration be 0.5,1.0,1.5, the tobacco bud essential oil of 2.0mg/mL, BHT solution is as sample solution (BHT is positive control).Extracting sample solution 0.5mL, add 50 μ L tween 80s respectively, 1mLPBS buffered soln (pH=7.4), 1mL concentration is the phenanthroline solution of 7.5mmol/L, 1mL concentration is the copperas solution of 3.25mmol/L, 2mL concentration is the hydrogen peroxide of 1.5%, fully at 37 DEG C, places 1h after mixing, surveys absorbance A at 536nm place.Control group distilled water replaces each sample, repeats aforesaid operations, surveys absorbance A at 536nm place
1, blank group replaces phenanthroline, copperas solution and hydrogen peroxide with distilled water, repeats aforesaid operations, surveys absorbance A at 536nm place
0.Often organize test and establish three groups of parallel tests.
The method of calculation of tobacco bud essential oil to OH free radical scavenging activity are as follows:
OH clearance rate (%)=[(A-A
0)/(A
1-A
0)] * 100%
Also compare with BHT in test, as shown in Figure 2, in concentration 0.5mg/mL ~ 2mg/mL, the OH Scavenging activity of tobacco bud essential oil does not have notable difference to result, all higher than the clearance rate of BHT.Illustrate that Chongqing Fengjie tobacco bud essential oil is relatively more remarkable to the scavenging capacity of hydroxyl radical free radical, be a kind of good antioxidant, can antioxidant be used as.
2) tobacco bud essential oil is to O
2-the mensuration of free radical scavenging activity:
Dehydrated alcohol with 50% respectively configuration concentration be 0.5,1.0,1.5, the tobacco bud essential oil of 2.0mg/mL, BHT solution is as sample solution (BHT is positive control).Sample solution gets 1mL, add 50 μ L tween 80s respectively, 4.5mL concentration is the Tris-HCL damping fluid (pH=8.2) of 0.05mol/L, 1mL concentration is the EDTA solution of 1mmol/L, 0.4mL concentration is the pyrogallol solution of 25mmol/L, after being placed in 25 DEG C of water-bath constant temperature 4min, be rapidly the HCL termination reaction of 12mol/L by 1mL concentration.Absorbance A is surveyed at 320nm place.Control group distilled water replaces each sample, repeats aforesaid operations, surveys absorbance A at 320nm place
1.Blank group distilled water replaces EDTA, pyrogallol and HCl solution, repeats aforesaid operations, surveys absorbance A at 320nm place
0.Often organize test and establish three groups of parallel tests.
Tobacco bud essential oil is to O
2-the method of calculation of free radical scavenging activity are as follows:
Superoxide anion clearance rate (%)=[1-(A-A
0)/A
1] * 100%
Also compare with BHT in test, result as shown in Figure 3, the O of tobacco bud essential oil
2-clearance rate increases rapidly within the scope of 0.5 ~ 1mg/mL, advances the speed subsequently and slows down.When concentration is 0.5mg/mL, the O of BHT
2-clearance rate is 29.81 ± 0.23%, and the clearance rate of tobacco bud essential oil is 17.20 ± 0.19%, when concentration is 2mg/mL, and the O of BHT
2-clearance rate is 43.66 ± 1.54%, and the clearance rate of tobacco bud essential oil reaches 35.37 ± 1.27%, and both diminish gradually at gap.Illustrate that Chongqing Fengjie tobacco bud essential oil is to O
2-scavenging capacity relatively remarkable, be a kind of good antioxidant, can antioxidant be used as.Embodiment 4:
The mensuration of tobacco bud essential oil bacteriostasis property:
Filter paper enzyme is adopted to measure bacteriostatic action.With punch tool, filter paper is made the circular paper that diameter is 6mm, be placed in the test tube of clean dried, 121 DEG C of autoclaving 30min.Filter paper is put into essential oil-ethanolic soln that concentration is 140mg/mL by aseptic operating platform.Drawing 0.2mL mycetome concentration with aseptic liquid-transfering gun is the bacteria suspension of 106 ~ 107cfu/mL, is added drop-wise on the solid medium of fresh sterile, and coating evenly.With aseptic nipper, the filter paper being soaked with essential oil is attached to solid medium center, each bacterial classification replicate(determination) 3 times.Constant temperature culture (bacterium: 37 DEG C/24h), measures antibacterial circle diameter, compares fungistatic effect.The benzylpenicillin sodium of employing 3mg/mL and the cefotaxime sodium of 10mg/mL are as positive control, and the ethanolic soln of 100% is negative control.Measure antibacterial circle diameter, compare fungistatic effect.
Adopt benzylpenicillin sodium, cefotaxime sodium and 100% ethanolic soln sample in contrast in test, result is as shown in table 2.The inhibition zone of 100% dehydrated alcohol is 0, is because ethanol has volatility, is evaporated, does not thus have biocidal property at the bacterial growth initial stage, also illustrate that the inhibition zone of Chongqing Fengjie is that essential oil produces simultaneously.Chongqing Fengjie essential oil is obvious to the fungistatic effect of intestinal bacteria, subtilis, Paenibacillus polymyxa, and antibacterial circle diameter reaches the half of benzylpenicillin sodium antibacterial circle diameter.Illustrate that Chongqing Fengjie tobacco bud essential oil has the ability of good bacteria growing inhibiting, can fungistat be used as.
The antibacterial circle diameter (mm) of table 2 different strain
The mensuration of tobacco bud essential oil MIC and MBC:
Micro-dilution method is utilized to measure minimal inhibitory concentration in 96 orifice plates.First first row eight holes add 200 μ L liquid nutrient mediums respectively and do blank, and secondary series adds the tobacco bud essential oil that 180 μ L liquid nutrient mediums and 20 μ L concentration are 140mg/mL, all adds the liquid nutrient medium of 100 μ L from the 3rd row to the 11 row.From secondary series, draw the three row each hole of corresponding 100 μ L essential oils to correspondence respectively, be diluted to the 11 row successively, finally from the 11 row, sucking-off 100 μ L discards.The concentration of each Liejing's oil be (14,7,3.5,1.75,0.875,0.4375,0.2188,0.1094,0.0547,0.0273mg/mL).The most backward two to the ten one each holes of row add 100 μ L bacteria suspensions, wherein fourth line adds 100 μ L nutrient solutions and contrasts, the 12 each hole of row adds 200 μ L bacterium liquid and does negative control, and the fourth line of bacterium does positive control with the doubling dilution of the benzylpenicillin sodium of 3mg/mL, constant temperature culture 24h at 37 DEG C.
In MIC determination test, 20 μ L spread plates will be taken out in the liquid nutrient medium of asepsis growth, continue to cultivate (37 DEG C/24h).Observe with or without colony growth.Cmin without colony growth is MBC.
Adopt benzylpenicillin sodium in test in contrast, result is as shown in table 3, benzylpenicillin sodium and the fungistatic effect of Chongqing Fengjie essential oil to bacterium (intestinal bacteria, subtilis and Paenibacillus polymyxa) all more remarkable.Wherein Chongqing Fengjie tobacco bud essential oil is comparatively strong to intestinal bacteria and subtilis bacteriostasis, and both MIC are 3.5mg/mL, and colibacillary MBC is 7mg/mL, and the MBC of subtilis is 3.5mg/mL.Illustrate that Chongqing Fengjie tobacco bud essential oil has the ability of good bacteria growing inhibiting, can be used as fungistat.
Table 3 minimum-bacteriostat mass concentration (MIC) and minimal bactericidal concentration (MBC)
Claims (8)
1. one kind with Chongqing Fengjie cloud and mist 87 bud for raw material extracts the technique of essential oil, it is characterized in that, comprise the following steps: after Chongqing Fengjie cloud and mist 87 bud after pulverizing is added distilled water by solid-liquid ratio 1:10 ~ 1:30, cellulase is added by 2% ~ 10% of tobacco bud quality, after adding sodium-chlor by 5% ~ 25% of total mass, supersound process 10 ~ 40min, then adopts wet distillation 2-6h to prepare bud essential oil.
2. according to claim 1 a kind of with the technique of Chongqing Fengjie cloud and mist 87 bud for raw material extraction essential oil, it is characterized in that: solid-liquid ratio is 1:15 ~ 1:25, and 5% ~ 7% adds cellulase, and 10% ~ 14% adds sodium-chlor, supersound process 10 ~ 25min, wet distillation 4-5h.
3. according to claim 2 a kind of with Chongqing Fengjie cloud and mist 87 bud for raw material extracts the technique of essential oil, it is characterized in that, solid-liquid ratio is 1:20, and 6% adds cellulase, and 10% adds sodium-chlor, supersound process 15min, wet distillation 4h; Open separator lower end piston after cooling, moisture is slowly flowed out, collects oil reservoir with liquid-transfering gun, and with dichloromethane rinse extractor three times, washing fluid and oil reservoir are merged, adds anhydrous sodium sulphate and spend the night, after removing moisture, low-temperature rotary evaporates, and removing methylene dichloride obtains tobacco bud essential oil.
4. according to claim 1-3 arbitrary described a kind of be the tobacco bud essential oil that technique that raw material extracts essential oil prepares with Chongqing Fengjie cloud and mist 87 bud.
5. tobacco bud essential oil according to claim 4 is as the application of antioxidant.
6. ask the application described in 5 according to right, it is characterized in that to O
2-the removing of free radical and to the application as oxygenant in the removing of OH free radical.
7. tobacco bud essential oil according to claim 4 is as the application of fungistat.
8. ask the application described in 7 according to right, it is characterized in that suppressing the application in intestinal bacteria, subtilis or Paenibacillus polymyxa.
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| CN108125277A (en) * | 2018-03-21 | 2018-06-08 | 福建中烟工业有限责任公司 | A kind of electronic cigarette and preparation method thereof |
| CN108315352A (en) * | 2018-01-31 | 2018-07-24 | 湖北中烟工业有限责任公司 | A kind of Enzyme treatment prepares the method and its application of lavender oil |
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