CN106047949A - Method for preparing butanol by utilizing soybean straws - Google Patents
Method for preparing butanol by utilizing soybean straws Download PDFInfo
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- CN106047949A CN106047949A CN201610544812.1A CN201610544812A CN106047949A CN 106047949 A CN106047949 A CN 106047949A CN 201610544812 A CN201610544812 A CN 201610544812A CN 106047949 A CN106047949 A CN 106047949A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention discloses a method for preparing butanol by utilizing soybean straws and belongs to the technical field of biomass energy. The butanol is prepared through the steps including soybean straw drying, crushing, pretreatment, hydrolysis, fermentation and the like. Raw materials for the method are wide in source, and the cost for preparing the butanol is low. A preparation process is simple, the input is low, and the method is suitable for large-scale popularization and application. Compared with fuel prepared by means of a petroleum refining method, the bio-fuel butanol prepared by means of the method has a remarkable environmentally friendly effect and can effectively reduce emissions of greenhouse gases. In addition, a novel solution is provided for the energy shortage problem.
Description
[technical field]
The invention belongs to biomass energy technology field, be specifically related to a kind of method utilizing soybean stalk to prepare butanol.
[background technology]
China's recent two decades comes due to coal, electricity, universal, various industrial products abundant of natural gas, and rural area is to soybean stalk
Demand reduces, a large amount of soybean stalks be processed into for a serious social problem, a lot of local peasants are still directly on ground
In burn soybean stalk, cause air pollution, fire, aircraft cannot the consequence such as Normal Take-Off And Landing.
Along with the growth of population, grain, energy and environment are the main challenges of facing mankind.Produce with cellulosic material
Renewable and clean energy resource is increasingly subject to extensive concern, and is considered as to have most with agricultural waste material for raw material production Fuel butanol
The industry of development prospect.Semen sojae atricolor is very wide in China's cultivated area, the substantial amounts of soybean stalk of annual generation.Protein in soybean stalk
Content is low but carbohydrate content high, and individual plants carbohydrate content is up to the 77.4% of dry, thus is to produce life
The quality raw materials of material energy.The research both at home and abroad soybean stalk converting Fuel butanol starts to walk, and current butanol productivity also compares
Low, it is the most undesirable that bottleneck is that soybean stalk is degraded into the technology of fermentable sugars.Therefore, the efficient degradation work of invention soybean stalk
Skill is respectively provided with high economic worth and social benefit to the comprehensive exploitation of soybean stalk and the recovery energy of lignocellulose.
Utilize lignocellulosic material to produce biological butanol has important meaning (biological to alleviating energy crisis and ecological deterioration
Matter Chemical Engineering, 2012,46 (3), 39-44).But during enzyme hydrolysis, due to portion of cellulose enzyme and hemicellulase not
Reversible adsorbs the loss that can cause enzymatic activity on substrate particularly lignin surface, cellulose in addition in the way of ineffective adsorption
The crystalline texture of itself and the complexity of hemicellulose structure are also the key factors causing hydrolysis efficiency low.
In actual applications, bio-fuel butanol can mix, again without carrying out vehicle with any ratio by gasoline due to it
Transformation, and its economy is high, can be effectively improved fuel efficiency and the distance travelled of vehicle.Therefore bio-fuel butanol compares bio-ethanol
The most superior quality and higher calorific value conversion ratio.Thus, bio-fuel butanol will more win the favor of people.Pass
The preparation method of the biological butanol of system is prepared from by fermentation with grains such as Semen Maydis, wheat and barley, Semen sojae atricolor for raw material.But
Not only can not satisfy social needs with grain for raw material production bio-fuel, and entail dangers to grain security.Research worker is had to refer to
Go out, even if all Semen Maydiss of U.S.'s plantation and Semen sojae atricolor are all used for producing bioenergy, also can only meet American society's gasoline respectively
The 12% and the 6% of diesel oil demand of demand.And Semen Maydis and Semen sojae atricolor first have to meet grain, feedstuff and other economic needs, can not
Can all be used for producing bio-fuel.International Monetary Fund also warns, the whole world produces constantly for the corn of bio-fuel
Increase likely world's poverty to be produced and have a strong impact on.To this end, research worker is all being tried to explore with non-grain class plant production
The method of bio-fuel butanol.
[summary of the invention]
The technical problem to be solved in the present invention is to provide a kind of method utilizing soybean stalk to prepare butanol, to solve soybean stalk
It is degraded into fermentable sugars and not exclusively causes the problems such as butanol yield is low.Raw material sources of the present invention is wide, prepares butanol low cost；This
Present invention is simple, less investment, is suitable for large-scale promotion and application；Bio-fuel fourth prepared by the method for the present invention
Alcohol has significant environmental protection effect compared with fuel prepared by petroleum refinery processes, and it can effectively reduce the discharge of greenhouse gases.
Meanwhile, also a kind of new solution is provided for energy scarcity problem.
In order to solve above technical problem, the present invention by the following technical solutions:
A kind of method utilizing soybean stalk to prepare butanol, comprises the following steps:
S1: use saline solution rinsing soybean stalk 2-3h, after rinsing, soybean stalk is placed at temperature is 97-105 DEG C dry to
Water content≤9%, prepares dry soybean stalk；
S2: dry soybean stalk step S1 prepared is pulverized, and sifts out, and prepares soybean stalk granule；
S3: put in microwave reactor by the soybean stalk granule that step S2 prepares, adds solution A mix homogeneously, is heated to 60-
64 DEG C, pretreatment 0.9-1.3h under speed of agitator is 200-300r/min, filters after being then cooled to room temperature, prepares pretreatment
Soybean stalk granule, the natural polymers such as the cellulose of soybean stalk, lignin can mutually dissociate by pretreatment, under being conducive to
The hydrolysis of one step；Described solution A is acetone, ethanol, the mixed liquor of ammonium lauryl sulfate salt, and the mass concentration of described acetone is
8%-10%, the mass concentration of described ethanol is 60%-80%, and the mass concentration of described ammonium lauryl sulfate salt is 6%-8%, described
Acetone soln, ethanol solution, the volume ratio of ammonium lauryl sulfate saline solution are 10-20:12-25:1-2, described soybean stalk
Granule is 1:10-13g/mL with the solid-liquid ratio of solution A；Ammonium lauryl sulfate salt and acetone, ethanol is mixed makes lignin
Separation has potentiation；
S4: the soybean stalk granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mixing all
Even, regulation pH is 1.2-1.6, is heated to 70-74 DEG C, processes 1.6-1.9h, then under speed of agitator is 300-400r/min
Filter after being cooled to room temperature, prepare sugar liquid；Described solution B is the mixed liquor of carbon back potassium sulfonate, sulphuric acid, described carbon back potassium sulfonate
Mass concentration is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, described carbon back potassium sulfonate solution, the volume ratio of sulfuric acid solution
For 6-10:5-8, the soybean stalk granule of described pretreatment and the solid-liquid ratio of solution B are 1:4-8g/mL；
S5: sugar liquid step S4 prepared accesses clostridium acetobutylicum, adds soybean starch, yeast extract, peptone, fat, sweet
Propylhomoserin, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, controlling temperature is 25-42 DEG C of bottom fermentation 30-100h, separated, smart
Evaporate prepared butanol；Described clostridium acetobutylicum quality is the 6%-10% of sugar liquid quality, described soybean starch, yeast extract, peptone,
Fat, glycine, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality be respectively sugar liquid quality 0.02%-0.04%,
0.01%-0.03%、0.01%-0.03%、0.03%-0.05%、0.04%-0.06%、0.04%-0.06%、0.18%-0.21%、
0.08%-0.12%、0.12%-0.16%。
Further, the concentration of saline solution described in step S1 is 10%-15%.
Further, the mesh number of sieve described in step S2 is 120-200.
Further, the temperature of pretreatment in microwave reactor of soybean stalk granule described in step S3 is 64 DEG C, turns
Speed is 300r/min, and the time is 0.9h.
Further, soybean stalk granule described in step S3 is 1:12g/mL with the solid-liquid ratio of solution A.
Further, the soybean stalk granule of pretreatment described in step S4 is 1.6 at the pH that ultrasound reactor processes,
Temperature is 74 DEG C, and rotating speed is 400r/min, and the time is 1.6h.
Further, the soybean stalk granule of pretreatment described in step S4 and the solid-liquid ratio of solution B are 1:6g/mL.
Further, clostridium acetobutylicum quality described in step S5 is the 8% of sugar liquid quality, described soybean starch, yeast
Cream, peptone, fat, glycine, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively sugar liquid quality
0.03%、0.02%、0.02%、0.04%、0.05%、0.05%、0.2%、0.1%、0.14%。
The method have the advantages that
(1) raw material sources of the present invention is wide, prepares butanol low cost；
(2) preparation technology of the present invention is simple, less investment, is suitable for large-scale promotion and application；
(3) uncertainty of microbial enzyme fermentation sugar refining technology can be avoided, reduce technical risk；
(4) sugar conversion ratio is high, and the level of residue of generation is few, is environmental type technique；
(5) hydrolysis temperature is relatively low, and energy resource consumption is few, beneficially the promotion and application of correlation technique；
(6) the sugared yield that this method prepares is 92.21%-92.92%, and butanol yield is 66.59%-67.47%, butanol purity
For 99.51%-99.56%, it is seen that the method using the present invention can high yield butanol；
(7) the bio-fuel butanol prepared by the method for the present invention has significantly compared with fuel prepared by petroleum refinery processes
Environmental protection effect, it can effectively reduce the discharge of greenhouse gases.Meanwhile, also a kind of new solution party is provided for energy scarcity problem
Case.
[detailed description of the invention]
For ease of being more fully understood that the present invention, being illustrated by following example, these embodiments belong to the protection of the present invention
Scope, but it is not intended to protection scope of the present invention.
In an embodiment, the described method utilizing soybean stalk to prepare butanol, comprise the following steps:
S1: the saline solution rinsing soybean stalk 2-3h using concentration to be 10%-15%, soybean stalk is placed on after rinsing temperature is
Dry to water content≤9% at 97-105 DEG C, prepare dry soybean stalk；
S2: dry soybean stalk step S1 prepared is pulverized, and crosses 120-200 mesh sieve, prepares soybean stalk granule；
S3: put in microwave reactor by the soybean stalk granule that step S2 prepares, adds solution A mix homogeneously, is heated to 60-
64 DEG C, pretreatment 0.9-1.3h under speed of agitator is 200-300r/min, filters after being then cooled to room temperature, prepares pretreatment
Soybean stalk granule；Described solution A is acetone, ethanol, the mixed liquor of ammonium lauryl sulfate salt, and the quality of described acetone is dense
Degree is 8%-10%, and the mass concentration of described ethanol is 60%-80%, and the mass concentration of described ammonium lauryl sulfate salt is 6%-8%,
Described acetone soln, ethanol solution, the volume ratio of ammonium lauryl sulfate saline solution are 10-20:12-25:1-2, described Semen sojae atricolor
Stalk particle is 1:10-13g/mL with the solid-liquid ratio of solution A；
S4: the soybean stalk granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mixing all
Even, regulation pH is 1.2-1.6, is heated to 70-74 DEG C, processes 1.6-1.9h, then under speed of agitator is 300-400r/min
Filter after being cooled to room temperature, prepare sugar liquid；Described solution B is the mixed liquor of carbon back potassium sulfonate, sulphuric acid, described carbon back potassium sulfonate
Mass concentration is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, described carbon back potassium sulfonate solution, the volume ratio of sulfuric acid solution
For 6-10:5-8, the soybean stalk granule of described pretreatment and the solid-liquid ratio of solution B are 1:4-8g/mL；
S5: sugar liquid step S4 prepared accesses clostridium acetobutylicum, adds soybean starch, yeast extract, peptone, fat, sweet
Propylhomoserin, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, controlling temperature is 25-42 DEG C of bottom fermentation 30-100h, separated, smart
Evaporate prepared butanol；Described clostridium acetobutylicum quality is the 6%-10% of sugar liquid quality, described soybean starch, yeast extract, peptone,
Fat, glycine, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality be respectively sugar liquid quality 0.02%-0.04%,
0.01%-0.03%、0.01%-0.03%、0.03%-0.05%、0.04%-0.06%、0.04%-0.06%、0.18%-0.21%、
0.08%-0.12%、0.12%-0.16%。
Below by more specifically embodiment, the present invention will be described.
Embodiment 1
A kind of method utilizing soybean stalk to prepare butanol, comprises the following steps:
S1: the saline solution rinsing soybean stalk 2.5h using concentration to be 12%, it is 97-105 that soybean stalk is placed on after rinsing temperature
Dry at DEG C to water content≤9%, prepare dry soybean stalk；
S2: dry soybean stalk step S1 prepared is pulverized, and crosses 150 mesh sieve, prepares soybean stalk granule；
S3: put in microwave reactor by the soybean stalk granule that step S2 prepares, adds solution A mix homogeneously, is heated to 62
DEG C, pretreatment 1.1h under speed of agitator is 300r/min, filters after being then cooled to room temperature, prepares the soybean stalk of pretreatment
Granule；Described solution A is acetone, ethanol, the mixed liquor of ammonium lauryl sulfate salt, and the mass concentration of described acetone is 9%, institute
The mass concentration stating ethanol is 70%, and the mass concentration of described ammonium lauryl sulfate salt is 7%, and described acetone soln, ethanol are molten
Liquid, the volume ratio of ammonium lauryl sulfate saline solution are 15:18:1, and described soybean stalk granule is 1 with the solid-liquid ratio of solution A:
12g/mL；
S4: the soybean stalk granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mixing all
Even, regulation pH is 1.4, is heated to 72 DEG C, processes 1.8h, be then cooled to mistake after room temperature under speed of agitator is 400r/min
Filter, prepares sugar liquid；Described solution B is the mixed liquor of carbon back potassium sulfonate, sulphuric acid, and the mass concentration of described carbon back potassium sulfonate is 6%,
The mass concentration of described sulphuric acid is 5%, and described carbon back potassium sulfonate solution, the volume ratio of sulfuric acid solution are 8:6, described pretreatment
Soybean stalk granule is 1:6g/mL with the solid-liquid ratio of solution B；
S5: sugar liquid step S4 prepared accesses clostridium acetobutylicum, adds soybean starch, yeast extract, peptone, fat, sweet
Propylhomoserin, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, controlling temperature is 35 DEG C of bottom fermentation 60h, and separated, rectification prepares
Butanol；Described clostridium acetobutylicum quality is the 8% of sugar liquid quality, described soybean starch, yeast extract, peptone, fat, sweet ammonia
Acid, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality is respectively the 0.03% of sugar liquid quality, 0.02%, 0.02%,
0.04%、0.05%、0.05%、0.2%、0.1%、0.14%。
Embodiment 2
A kind of method utilizing soybean stalk to prepare butanol, comprises the following steps:
S1: the saline solution rinsing soybean stalk 3h using concentration to be 10%, after rinsing, soybean stalk is placed on temperature and is 97-105 DEG C
Lower drying, to water content≤9%, prepares dry soybean stalk；
S2: dry soybean stalk step S1 prepared is pulverized, and crosses 120 mesh sieve, prepares soybean stalk granule；
S3: put in microwave reactor by the soybean stalk granule that step S2 prepares, adds solution A mix homogeneously, is heated to 60
DEG C, pretreatment 1.3h under speed of agitator is 200r/min, filters after being then cooled to room temperature, prepares the soybean stalk of pretreatment
Granule；Described solution A is acetone, ethanol, the mixed liquor of ammonium lauryl sulfate salt, and the mass concentration of described acetone is 8%, institute
The mass concentration stating ethanol is 60%, and the mass concentration of described ammonium lauryl sulfate salt is 6%, and described acetone soln, ethanol are molten
Liquid, the volume ratio of ammonium lauryl sulfate saline solution are 10:12:1, and described soybean stalk granule is 1 with the solid-liquid ratio of solution A:
10g/mL；
S4: the soybean stalk granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mixing all
Even, regulation pH is 1.2, is heated to 70 DEG C, processes 1.9h, be then cooled to mistake after room temperature under speed of agitator is 300r/min
Filter, prepares sugar liquid；Described solution B is the mixed liquor of carbon back potassium sulfonate, sulphuric acid, and the mass concentration of described carbon back potassium sulfonate is 5%,
The mass concentration of described sulphuric acid is 4%, and described carbon back potassium sulfonate solution, the volume ratio of sulfuric acid solution are 6:5, described pretreatment
Soybean stalk granule is 1:4g/mL with the solid-liquid ratio of solution B；
S5: sugar liquid step S4 prepared accesses clostridium acetobutylicum, adds soybean starch, yeast extract, peptone, fat, sweet
Propylhomoserin, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, controlling temperature is 25 DEG C of bottom fermentation 100h, and separated, rectification prepares
Butanol；Described clostridium acetobutylicum quality is the 6%-10% of sugar liquid quality, described soybean starch, yeast extract, peptone, fat,
Glycine, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality be respectively the 0.02% of sugar liquid quality, 0.01%, 0.01%,
0.03%、0.04%、0.04%、0.18%、0.08%、0.12%。
Embodiment 3
A kind of method utilizing soybean stalk to prepare butanol, comprises the following steps:
S1: the saline solution rinsing soybean stalk 2h using concentration to be 15%, after rinsing, soybean stalk is placed on temperature and is 97-105 DEG C
Lower drying, to water content≤9%, prepares dry soybean stalk；
S2: dry soybean stalk step S1 prepared is pulverized, and crosses 200 mesh sieve, prepares soybean stalk granule；
S3: put in microwave reactor by the soybean stalk granule that step S2 prepares, adds solution A mix homogeneously, is heated to 64
DEG C, pretreatment 0.9h under speed of agitator is 300r/min, filters after being then cooled to room temperature, prepares the soybean stalk of pretreatment
Granule；Described solution A is acetone, ethanol, the mixed liquor of ammonium lauryl sulfate salt, and the mass concentration of described acetone is 10%, institute
The mass concentration stating ethanol is 80%, and the mass concentration of described ammonium lauryl sulfate salt is 8%, and described acetone soln, ethanol are molten
Liquid, the volume ratio of ammonium lauryl sulfate saline solution are 20:25:2, and described soybean stalk granule is 1 with the solid-liquid ratio of solution A:
13g/mL；
S4: the soybean stalk granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mixing all
Even, regulation pH is 1.6, is heated to 74 DEG C, processes 1.6h, be then cooled to mistake after room temperature under speed of agitator is 400r/min
Filter, prepares sugar liquid；Described solution B is the mixed liquor of carbon back potassium sulfonate, sulphuric acid, and the mass concentration of described carbon back potassium sulfonate is 7%,
The mass concentration of described sulphuric acid is 6%, and described carbon back potassium sulfonate solution, the volume ratio of sulfuric acid solution are 10:8, described pretreatment
Soybean stalk granule is 1:8g/mL with the solid-liquid ratio of solution B；
S5: sugar liquid step S4 prepared accesses clostridium acetobutylicum, adds soybean starch, yeast extract, peptone, fat, sweet
Propylhomoserin, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, controlling temperature is 42 DEG C of bottom fermentation 30h, and separated, rectification prepares
Butanol；Described clostridium acetobutylicum quality is the 6%-10% of sugar liquid quality, described soybean starch, yeast extract, peptone, fat,
Glycine, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality be respectively the 0.04% of sugar liquid quality, 0.03%, 0.03%,
0.05%、0.06%、0.06%、0.21%、0.12%、0.16%。
The sugar liquid taking out appropriate embodiment 1-3 is boiled, is inactivated, and appropriateness dilution, measures sugar fermentation by DNS colorimetric fixed sugar method
Content, and calculate sugar yield；The method of detection embodiment 1-3 prepares butanol yield and purity simultaneously, and result is as shown in the table:
| Embodiment | Sugar yield, % | Butanol yield, % | Butanol purity, % |
| 1 | 92.85 | 67.16 | 99.55 |
| 2 | 92.21 | 66.59 | 99.51 |
| 3 | 92.92 | 67.47 | 99.56 |
As seen from the above table, the method for embodiment of the present invention 1-3 the sugared yield prepared is 92.21%-92.92%, and butanol yield is
66.59%-67.47%, butanol purity is 99.51%-99.56%, illustrates that sugar conversion ratio is high, and the method using the present invention can high yield
Butanol.
Above content it cannot be assumed that the present invention be embodied as be confined to these explanation, technology belonging to the present invention is led
For the those of ordinary skill in territory, without departing from the inventive concept of the premise, it is also possible to make some simple deduction or replace,
All should be considered as belonging to the scope of patent protection that the present invention is determined by the claims submitted to.
Claims (8)
1. one kind utilizes the method that soybean stalk prepares butanol, it is characterised in that comprise the following steps:
S1: use saline solution rinsing soybean stalk 2-3h, after rinsing, soybean stalk is placed at temperature is 97-105 DEG C dry to
Water content≤9%, prepares dry soybean stalk；
S2: dry soybean stalk step S1 prepared is pulverized, and sifts out, and prepares soybean stalk granule；
S3: put in microwave reactor by the soybean stalk granule that step S2 prepares, adds solution A mix homogeneously, is heated to 60-
64 DEG C, pretreatment 0.9-1.3h under speed of agitator is 200-300r/min, filters after being then cooled to room temperature, prepares pretreatment
Soybean stalk granule；Described solution A is acetone, ethanol, the mixed liquor of ammonium lauryl sulfate salt, and the quality of described acetone is dense
Degree is 8%-10%, and the mass concentration of described ethanol is 60%-80%, and the mass concentration of described ammonium lauryl sulfate salt is 6%-8%,
Described acetone soln, ethanol solution, the volume ratio of ammonium lauryl sulfate saline solution are 10-20:12-25:1-2, described Semen sojae atricolor
Stalk particle is 1:10-13g/mL with the solid-liquid ratio of solution A；
S4: the soybean stalk granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mixing all
Even, regulation pH is 1.2-1.6, is heated to 70-74 DEG C, processes 1.6-1.9h, then under speed of agitator is 300-400r/min
Filter after being cooled to room temperature, prepare sugar liquid；Described solution B is the mixed liquor of carbon back potassium sulfonate, sulphuric acid, described carbon back potassium sulfonate
Mass concentration is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, described carbon back potassium sulfonate solution, the volume ratio of sulfuric acid solution
For 6-10:5-8, the soybean stalk granule of described pretreatment and the solid-liquid ratio of solution B are 1:4-8g/mL；
S5: sugar liquid step S4 prepared accesses clostridium acetobutylicum, adds soybean starch, yeast extract, peptone, fat, sweet
Propylhomoserin, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, controlling temperature is 25-42 DEG C of bottom fermentation 30-100h, separated, smart
Evaporate prepared butanol；Described clostridium acetobutylicum quality is the 6%-10% of sugar liquid quality, described soybean starch, yeast extract, peptone,
Fat, glycine, vitamin, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality be respectively sugar liquid quality 0.02%-0.04%,
0.01%-0.03%、0.01%-0.03%、0.03%-0.05%、0.04%-0.06%、0.04%-0.06%、0.18%-0.21%、
0.08%-0.12%、0.12%-0.16%。
The method utilizing soybean stalk to prepare butanol the most according to claim 1, it is characterised in that eat described in step S1
The concentration of saline is 10%-15%.
The method utilizing soybean stalk to prepare butanol the most according to claim 1, it is characterised in that sieve described in step S2
The mesh number of son is 120-200.
The method utilizing soybean stalk to prepare butanol the most according to claim 1, it is characterised in that big described in step S3
Bean stalk particle temperature of pretreatment in microwave reactor is 64 DEG C, and rotating speed is 300r/min, and the time is 0.9h.
The method utilizing soybean stalk to prepare butanol the most according to claim 1, it is characterised in that big described in step S3
Bean stalk particle is 1:12g/mL with the solid-liquid ratio of solution A.
The method utilizing soybean stalk to prepare butanol the most according to claim 1, it is characterised in that pre-described in step S4
The soybean stalk granule processed is 1.6 at the pH that ultrasound reactor processes, and temperature is 74 DEG C, and rotating speed is 400r/min, the time
For 1.6h.
The method utilizing soybean stalk to prepare butanol the most according to claim 1, it is characterised in that pre-described in step S4
The soybean stalk granule processed is 1:6g/mL with the solid-liquid ratio of solution B.
The method utilizing soybean stalk to prepare butanol the most according to claim 1, it is characterised in that described in step S5 third
Ketone Clostridium acetobutylicum quality is the 8% of sugar liquid quality, described soybean starch, yeast extract, peptone, fat, glycine, vitamin, phosphorus
Acid dihydride sodium, magnesium nitrate, calcium sulfate quality be respectively the 0.03% of sugar liquid quality, 0.02%, 0.02%, 0.04%, 0.05%,
0.05%、0.2%、0.1%、0.14%。
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| CN201610544812.1A CN106047949A (en) | 2016-07-12 | 2016-07-12 | Method for preparing butanol by utilizing soybean straws |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101748158A (en) * | 2010-02-05 | 2010-06-23 | 南京工业大学 | Method for preparing biological butanol by fermenting lignocellulose biomass |
| CN102352381A (en) * | 2011-10-08 | 2012-02-15 | 山东龙力生物科技股份有限公司 | Method using xylose production waste liquid to produce acetone and butanol |
| CN103814135A (en) * | 2011-06-17 | 2014-05-21 | 布特马斯先进生物燃料有限责任公司 | Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101748158A (en) * | 2010-02-05 | 2010-06-23 | 南京工业大学 | Method for preparing biological butanol by fermenting lignocellulose biomass |
| CN103814135A (en) * | 2011-06-17 | 2014-05-21 | 布特马斯先进生物燃料有限责任公司 | Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation |
| CN102352381A (en) * | 2011-10-08 | 2012-02-15 | 山东龙力生物科技股份有限公司 | Method using xylose production waste liquid to produce acetone and butanol |
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Application publication date: 20161026 |