CN106047689B - Charge collection bacterium device and method - Google Patents
Charge collection bacterium device and method Download PDFInfo
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- CN106047689B CN106047689B CN201610412953.8A CN201610412953A CN106047689B CN 106047689 B CN106047689 B CN 106047689B CN 201610412953 A CN201610412953 A CN 201610412953A CN 106047689 B CN106047689 B CN 106047689B
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- bacterium
- metal bar
- becket
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- metal
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/07—Means for pre-treatment of biological substances by electrical or electromagnetic forces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- H—ELECTRICITY
- H02—GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
- H02K—DYNAMO-ELECTRIC MACHINES
- H02K7/00—Arrangements for handling mechanical energy structurally associated with dynamo-electric machines, e.g. structural association with mechanical driving motors or auxiliary dynamo-electric machines
- H02K7/06—Means for converting reciprocating motion into rotary motion or vice versa
Abstract
The present invention discloses the charge collection bacterium device and method in a kind of microorganism collection detection field, the front side of bacteriological filter film is equipped with the first metal bar, rear side is equipped with the second metal bar, the upper end of first metal bar and the second metal bar is commonly connected to horizontal movement device, it is that initial position, most left extreme position are off position to be moved to most right extreme position, it is arranged right below a collection bacterium becket in the first metal bar of rest position, being put immediately below collection bacterium becket has the collection bacterium metal basin for being marked with sterile water；Collect bacterium becket connection collection collarium lifting gear；Using charge, identical charges repel each other, the principle that there is a natural attraction between the sexes, the bacterium with faint negative electrical charge is first set to be attracted by the first positively charged metal bar, it is transferred on the first metal bar, collection bacterium becket is transferred to from the first metal bar again, finally from the sterile water for falling to collection bacterium metal basin on bacterium collecting becket, the bacterium on bacteriological filter film is had collected with existing a small amount of liquid.
Description
Technical field
The present invention relates to microorganism collection detection field, the device of bacterium in especially a kind of liquid using charge-trapping.
Background technology
It is advantageous to the growth of institute's aquaculture organism containing trace of bacteria in cultivation water, but bacterium is more than a certain amount of, may be led
Cause aquaculture organism dead.In cultivation water, the bacterial content of suitable aquaculture organism existence is very low directly to be detected.When application electricity
When impedance method detects bacterial concentration in liquid, the bacterial concentration in culture medium need to reach 105~106A/mL, if being less than this
Concentration can not then detect the variation of impedance.However, the bacterial concentration no more than 100/mL in cultivation water, if it exceeds
This concentration can make aquaculture organism illness, and this concentration is much unable to reach the requirement of electrical impedance method detection, so needing
Cultivation water is concentrated.When concentration, first cultivation water can be filtered with bacteriological filter film, the bacterium in cultivation water is detained
In the side of filter membrane.It needs to be collected into bacterium as much as possible on film with water as few as possible when handling filter membrane.Currently,
It is broadly divided into two classes by the method that filter membrane collects bacterium：One kind is that filter membrane input is equipped with sterile water（Or buffer solution）'s
In container, bead is housed in container, vibrates container, so that liquid is flowed, the bacterium on filter membrane is made to fall off, then take out filter
Film；Another kind of is with the directly flushed filter membrane of a large amount of water, and the liquid of flushing flows into a larger container.Though first kind method
Right water is less, but thalline cannot all fall off, and can also take the bacterium that a part is melted into liquid when taking-up filter membrane out of,
So bacterial concentration is not high in container；Second class method then with a large amount of liquid wash, causes the bacterial concentration in container very low, by
It can not see bacterium in naked eyes, when flushing can not also determine whether that all punching is fallen.So the liquid that above two method obtains
Still directly can not electrochemically it detect, it may there is still a need for cultures.
Invention content
To overcome the above disadvantages, the present invention provides a kind of charge collection bacterium device and method, according to charge, identical charges repel each other,
The principle that there is a natural attraction between the sexes realizes that bacterium is collected, so as to use less water to receive using the included faint negative electrical charge of bacterium itself
Collect bacterium, makes have higher bacterial concentration in the water being collected into.
Charge collection bacterium device of the present invention the technical solution adopted is that：Including the fixed flat membrane fixing bracket in a both ends,
Lower section is fixedly connected with a bacteriological filter film among film fixing bracket, and the antetheca band bacterium of bacteriological filter film, rear wall is without thin
Bacterium, the front side of bacteriological filter film is equipped with the first metal bar, rear side is equipped with the second metal bar, the first metal bar and the second metal bar
Upper end is commonly connected to horizontal movement device and drives left and right horizontal movement by horizontal movement device, is moved to most right limit position
To set be that initial position, most left extreme position are off position, and one is arranged right below in the first metal bar of rest position
A collection bacterium becket, the underface of collection bacterium becket, which is put, the collection bacterium metal basin for being marked with sterile water；Collect the connection of bacterium becket
Collection collarium lifting gear and being driven by collection collarium lifting gear vertically moves up and down, and collection bacterium becket is located at the in initial position
The underface of one metal bar, extreme position of the collection bacterium becket on most be upper limit position, most under extreme position be lower limit
It sets, the collection bacterium becket at lower position is immersed in collection bacterium metal basin sterile water；Two metal bars, collection bacterium becket, collection bacterium
Metal basin is connected by conducting wire with circuit controller respectively, and horizontal movement device sum aggregate collarium lifting gear is by circuit control
Device controls work.
The collection bacterium method of charge collection bacterium device of the present invention is the technical solution adopted is that comprise the steps of：
A, the first metal bar and the second metal bar be in initial position, circuit controller control the first metal bar it is positively charged,
Second metal bar is negatively charged, collection bacterium becket sum aggregate bacterium basin is not charged；
B, collection collarium lifting gear does not work, horizontal movement device work, drive the first metal bar and the second metal bar to
It moves left, makes the attraction and electronegative second metal of bacterium on bacteriological filter film simultaneously by the first positively charged metal bar
The repulsion of stick, on from bacteriological filter film transfer to the first metal bar；
C, when the first metal bar and the second metal bar reach most left rest position, horizontal movement device is stopped,
Circuit controller the second metal bar of control is not charged, the first metal bar is negatively charged, collection bacterium endless metal belt positive electricity, collection bacterium metal basin
It is not charged；Collect the work of collarium lifting gear, after driving collection bacterium becket to be first moved upward to upper limit position by initial position
Initial position is returned to still further below, and the collection coaxial empty set of bacterium becket is vertically transported outside the first metal bar and along the first metal bar
Dynamic, bacterium is transferred to from the first metal bar on collection bacterium becket；
D, after collection bacterium metal is looped back to initial position, circuit controller control the first metal bar, the second metal bar it is not charged,
It is positively charged to collect bacterium endless metal belt negative electricity, collection bacterium metal basin；Collect collarium lifting gear drive collection bacterium becket from initial position to
It is moved at lower position, collection bacterium becket is completely immersed in the sterile water of collection bacterium metal basin, and bacterium is golden from bacterium collecting
Belong in the sterile water for falling to collection bacterium metal basin on ring.
The present invention has the following advantages that compared with existing methods and techniques：
1, identical charges repel each other using charge by the present invention, the principle that there is a natural attraction between the sexes, makes band on the first metal bar with controller control
Positive electricity, it is negatively charged on the second metal bar, so that the bacterium with faint negative electrical charge is attracted by the first positively charged metal bar, while by band
Second metal bar of negative electricity repels, and is transferred to from bacteriological filter film on the first metal bar.It is insufficient to prevent bacterium from collecting, make
First metal bar and the second metal bar stop after moving back and forth repeatedly, more bacteriums can be collected, in this way, making to be dispersed in bacterium
Bacterium on filter membrane has been concentrated on the first metal bar.
2, the diameter of the collection bacterium becket in the present invention is more bigger than the first metal bar width, for collecting the first metal bar
Bacterium, enable to integrate bacterium becket and moved up and down using the first metal bar as axle center, without being in direct contact with metal bar；Becket
Also very little, the area for collecting bacterium becket inner wall are more much smaller than the first metal bar lateral area for height.Using identical charges repel each other, there is a natural attraction between the sexes
Principle, the first metal bar is negatively charged, collect bacterium endless metal belt positive electricity, so that collection bacterium becket is moved up and down first by controller
Bacterium on metal bar is transferred on the inside of becket, and bacterium is concentrated again.
3, the present invention collects bacterium as much as possible with water as few as possible, keeps the bacterial concentration in liquid higher, institute
It is cylindrical metal basin to collect bacterium metal basin, the diameter of basin mouth is larger than the diameter for collecting bacterium becket, makes collection bacterium metal
Ring is only contacted with water when entering collection bacterium metal basin, but metal basin contact of getting along well.The holder sum aggregate bacterium of fixed set bacterium becket
The part that becket is in contact needs in the water for enabling collection bacterium becket be immersed in collection bacterium metal basin vertically downward.
4, the stride value of the linear stepping motor used in the present invention is not interfered by external factor, and the accumulation of every revolution misses
Difference is zero, so stepper motor can relatively accurately determine the position of nut, and step motor control performance is good, start, stop,
Overturning be all completed in a few pulses, so in the present invention first motor and the second motor repeatedly rotate and reverse transformation can
It is rapidly and accurately controlled by MCU control systems.
5, apparatus of the present invention concentrate the bacterial liquid of collection to a certain extent, existing few in bacterium metal basin with collecting
Quantity of fluid has collected the bacterium on bacteriological filter film, and improves automatization level.
Description of the drawings
Fig. 1 is the overall structure diagram of charge collection bacterium device of the present invention；
Fig. 2 is the fixed structure chart of bacteriological filter film in Fig. 1；
Fig. 3 is that the right side of the first nut and nut seat 8 regards schematic enlarged-scale view in Fig. 1；
Fig. 4 is the motion process schematic diagram of apparatus of the present invention shown in Fig. 1；
Fig. 5 is the collection bacterium Product management model figure for collecting bacterium becket 24 in Fig. 1；
Fig. 6 is the internal structure of circuit controller 15 and external block diagram in Fig. 1；
The serial number and title of each component in attached drawing：1. wall, 2. wall tops, 3. first motors, 4. first shaft couplings, 5.
One bearing, 6. first guide rails, 7. first lead screw shaft, 8. first nuts and nut seat, 9. second bearings, 10. film fixing brackets,
11. film geometrical clamp, 12. bacteriological filter films, 13. first metal bars, 14. second metal bars, 15. circuit controllers, 16. second electricity
Machine, 17. second shaft couplings, 18. 3rd bearings, 19. second lead screw shaft, 20. second nuts and nut seat, 21. fourth bearings,
22. the second guide rail, 23. collection collarium fixed frames, 24. collection bacterium beckets, 25. collection bacterium metal basins, 26. conducting wires, 27. holders are fixed
Seat, 28. leading screw axis holes, 29. guide-track grooves, 30. first metal bar fixed seats, 31. second metal bar fixed seats.
Specific implementation mode
Referring to Fig. 1 and Fig. 2, charge collection bacterium device of the present invention includes a horizontal film fixing bracket 10, film fixing bracket
10 left and right ends are fixed by the bracket the fixation of seat 27 respectively, see Fig. 2.The left and right ends of rack fixing seat 27 are separately fixed at a left side
1 inside of right wall.A bacteriological filter film 12 is fixed with two film geometrical clamps 11 among film fixing bracket 10, by bacteriological filter
Film 12 is fixed on 10 lower section of film fixing bracket.The antetheca band bacterium of bacteriological filter film 12, rear wall is without bacterium.In film fixing bracket
10 and bacteriological filter film 12 front side and right side respectively there are one vertical metal bar, be respectively front side the first metal bar 13 and after
Second metal bar 14 of side, the first metal bar 13 is in side of the bacteriological filter film 12 with bacterium, and the second metal bar 14 is in bacterium mistake
Side of the filter membrane 12 without bacterium.Two metal bars before and after film fixing bracket 10 and bacteriological filter film 12 relative to being arranged symmetrically.
Longitudinal separation between two metal bars is D, and the longitudinal separation between two metal bars and film fixing bracket 10 is D/2.Two
A metal bar is connected by respective conducting wire with circuit controller 15 respectively, and 13 He of the first metal bar is controlled by circuit control box 15
The charging property of second metal bar 14.
The upper end of first metal bar 13 and the second metal bar 14 is commonly connected to horizontal movement device, by horizontal movement device
Two metal bars are driven to make the horizontal movement on left and right directions in the both sides of bacteriological filter film 12.
Horizontal movement device is fixed on 2 lower section of wall top, including a first shaft coupling 4, one of first motor 3, one
7, first nuts of the first lead screw shaft of 6, one, the first guide rail of first bearing 5, two and 8, second bearings 9 of nut seat.
Wherein, the output shaft of the first lead screw shaft 7, the first guide rail 6 and first motor 3 is horizontally disposed, and first motor 3 is straight line stepping
The output shaft of motor, first motor 3 is coaxially connected by first shaft coupling 4 and the first lead screw shaft 7, drives the rotation of the first lead screw shaft 7
Turn.The both ends of first lead screw shaft 7 are also fixed on 2 lower section of wall top with first bearing 5 and second bearing 9 respectively.In the first leading screw
The front and back side of axis 7 is a guide rail 6 respectively, two guide rails 6 relative to being arranged symmetrically before and after the first lead screw shaft 7, two guide rails 6
Both ends are separately fixed at 2 lower section of wall top.Cooperation the first nut of installation and nut seat 8, form leading screw spiral shell in the first lead screw shaft 7
Parent agency.First nut and 8 lower section of nut seat are fixedly connected with the upper end of the first metal bar 13 and the second metal bar 14 jointly.First
Motor 3 connects circuit controller 15 through control line, controls work by circuit controller 15, first motor 3 drives first after working
Lead screw shaft 7 rotates, to drive the first metal bar 13 and the second metal bar 14 left and right water simultaneously by the first nut and nut seat 8
Flat movement.
One collection bacterium becket 24 is set in the lower section of the first metal bar 13 of front side, in the underface of collection bacterium becket 24
Place collection bacterium metal basin 25.Collection bacterium becket 24 is driven by collection collarium lifting gear to be vertically moved up and down, can only be with when mobile
Axis moves up and down centered on first metal bar 13, but cannot be contacted with the first metal bar 13 or bacteriological filter film 12.Collect bacterium metal
Ring 24 is moved downward by the drive of collection collarium lifting gear, can be entered inside collection bacterium metal basin 25, but the two is not directly contacted with.Collection
Bacterium becket 24 is connected by a conducting wire with circuit controller 15, and collection bacterium metal basin 25 is also by a conducting wire and circuit
Controller 15 is connected.
Primary structure and the horizontal movement device for collecting collarium lifting gear are essentially identical, and collection collarium lifting gear is fixed on a left side
1 inside of side walls, and positioned in the front side of horizontal movement device.Including 16, second shaft couplings 17 of second motor,
One 19, second nut of the second lead screw shaft of 22, one, the second guide rail of 3rd bearing 18, two and nut seat 20, one
Four bearings 21.Wherein, the output shaft of the second lead screw shaft 19, the second guide rail 22 and the second motor 16 is arranged vertically, the second motor
16 be linear stepping motor, is fixed on 1 inside of left side wall, and the output shaft of the second motor 16 is coaxially connected by second shaft coupling 17
19 one end of the second lead screw shaft is connect, the rotation of the second lead screw shaft 19 is driven.The upper and lower ends of second lead screw shaft 19 pass through third axis respectively
Hold 18 and fourth bearing 21 be fixed on the inside of left wall 1.Second guide rail is respectively set in side before and after the second lead screw shaft 19
22, relative to being arranged symmetrically before and after the second lead screw shaft 19, two second guide rails 22 are fixed in left wall 1 two second guide rails 22
Side.Coaxial cooperation has the second nut and nut seat 20 in the second lead screw shaft 19.Second nut and nut seat 20 are fixedly connected with collection
23 one end of collarium fixed frame, 23 other end of collection collarium fixed frame stretch out to the lower section of the first metal bar 13 and are fixedly connected with collection bacterium gold
Belong to ring 24.Second motor 16 connects circuit controller 15 through control line, and work, 16 work of the second motor are controlled by circuit controller 15
The second lead screw shaft 19 is driven to rotate after work, to drive about 24 collection bacterium becket vertically to move by the second nut and nut seat 20
It is dynamic.
8 structure of first nut and nut seat shown in Figure 3, the first nut and 8 entirety of nut seat are a cuboids
Structure is opened therebetween there are one leading screw axis hole 28, for being connected with the first lead screw shaft 7.Front and back position above it is equipped with
Two guide-track grooves 29, it is mutually embedding respectively for two first guide rails 6, make the first nut and nut seat 8 under the drive of first motor 3
It is only to carry out horizontal movement without rotating.A metal bar is respectively set before and after the lower section of the first nut and nut seat 8 to fix
Seat, is the first metal bar fixed seat 30 and the second metal bar fixed seat 31 respectively, and the first metal bar fixed seat 30 is by the first metal
Stick 13 is fixed on the first nut and nut seat 8 close to the side of collection collarium lifting gear, and the second metal bar fixed seat 31 is by second
Metal bar 14 is fixed on the first side of the metal bar 13 far from collection collarium lifting gear.The structure of second nut and nut seat 20 with
First nut and 8 structure of nut seat are similar, are only that the position of fixed set collarium fixed frame 23 is different.
Referring to Fig. 4, horizontal movement device drives the first nut and nut seat 8 and the first metal bar 13, the second metal bar 14
Horizontal movement, it is initial position B to move to most right extreme position, moves to most left extreme position and is off position A.Initially
Horizontal distance between position B and stop position A is S.When the first nut and nut seat 8 are parked at stop position A, collection bacterium gold
The center for belonging to ring 24 is located at the underface of the first metal bar 13 at this time, and the center for collecting bacterium becket 24 and the first metal bar 13 is total
Line.The radius of collection bacterium becket 24 is less than D/2, and collection bacterium becket 24 is avoided to be contacted with bacteriological filter film 12.
Collecting collarium lifting gear drives the second nut and about 20 nut seat to move vertically, the extreme position under moving to most
It is lower position E, the extreme position moved on most is upper limit position C, the second nut and nut seat 20 in the second lead screw shaft 19
It is the initial position D of movement at the position of lower middle, when at initial position D, collection bacterium becket 24 is in the first metal bar 13
Immediately below center.Vertical range at upper limit position C and between initial position D is X, the places initial position D at the E of lower position
Between vertical range be Y.
The radius for collecting the radius ratio collection bacterium becket 24 of bacterium metal basin 25 is larger, the vertical height of collection bacterium metal basin 25
Degree is about collect bacterium becket vertical height 3~4 times, and collection bacterium becket 24 is enable all to submerge and not contact.In lower limit
When setting at E, collection bacterium becket 24 is fully immersed in collection bacterium metal basin 25.
First metal bar 13 and the second metal bar 14 are that rectangle is Metal Flake, and rectangular left and right width is L, metal
Piece face bacteriological filter film 12 makes the first metal bar 13 and the second metal bar 14 compared with the relative area of bacteriological filter film 12
Greatly, make its adsorbable more bacterium.
Referring to Fig. 5, collection bacterium becket 24 connects circuit controller 15 by conducting wire 26, in the second nut and nut seat 20 first
During being moved back and forth up and down between beginning position D and upper limit position C, collect 24 empty set of bacterium becket outside the first metal bar 13,
The central shaft of the two is conllinear, and collection bacterium becket 24 is moved back and forth along the first about 13 metal bar, by collection bacterium becket 24 come
The dynamic bacterium transfer by the first metal bar 13 is backhauled to transport on collection bacterium becket 24.
Referring to Fig. 6, circuit controller 15 is mounted on inside a controller box, and entire controller box is fixed on wall 1,
Circuit controller 15 is by a MCU control system, an electric supply system, a 5V DC power supply and four switching group groups
At.MCU control systems connect electric supply system, are powered by electric supply system.MCU control systems are separately connected first motor
3 and second motor 16, positive and negative rotation and the speed of first motor 3 and the second motor 16 are controlled, first motor 3 is made to drive the first metal
The both sides side-to-side movement of stick 13 and the second metal bar 14 in bacteriological filter film 12；The second motor 16 is set to drive on collection bacterium becket 24
Lower movement.MCU control systems are also connected with first switch group, second switch group, third switching group, the 4th switch respectively, control
Situation is switched on-off in switching group processed.The positive and negative anodes of 5V DC power supplies are connected on four switching group input terminals, and positive pole connects a left side
Side input terminal, power cathode connect right side input terminal.It is made of two switches inside switching group, break-make situation is controlled by MCU is
System determines.The output end of first switch group is connected with the first metal bar 13, controls the polarity of first 13 chargeds of metal bar；The
The output end of two switching groups is connected with the second metal bar 14, controls the charging property of the second metal bar 14；The output of third switching group
End is connected with collection bacterium becket 24, the charging property of domination set bacterium becket 24；The output end of 4th switching group and collection bacterium metal water
Basin 25 is connected, the charging property of domination set bacterium metal basin 25.
Before charge collection bacterium device of the present invention is started to work, the nothing of basin volume 2/3 is injected in collecting bacterium metal basin 25
Bacterium water enables collection bacterium becket 24 to be completely immersed in water, and anhydrous spilling after immersion, the sterile water ratio in collection bacterium metal basin 25
Directly wash away sterile water used in bacterial membrane to be lacked it is more.
When start-up operation, the first nut and nut seat 8, the first metal bar 13 and the second metal bar 14 are in most right initial
At the B of position, the first metal bar 13 in side of the bacteriological filter film 12 with bacterium, the second metal bar 14 bacteriological filter film 12 without
The side of bacterium, after powering on, circuit controller 15 is started to work.It is positively charged to control the first metal bar 13, the second metal bar
14 is negatively charged, at this point, collection bacterium 24 sum aggregate bacterium basins 25 of becket are not charged.Then, 15 controlled level sportswear of circuit controller
Set work, first motor 3 rotates forward, and the first nut and nut seat 8 is driven to move downward, and collection collarium lifting gear does not work, i.e., and the
Two motors 16 do not work, and the timer internal setting time of the MCU control systems of circuit controller 15 is periodicallyt 1, and start
Timing.First motor 3 drive the first nut and nut seat 8 from the B of position move downward, make to be fixed on the first nut and spiral shell
The first metal bar 13 and the second metal bar 14 in female seat 8 also move downward therewith, until being moved at stop position A.Movement
In the process, according to the charge principle that identical charges repel each other, there is a natural attraction between the sexes, itself carries the bacterium meeting of faint negative electricity on bacteriological filter film 12
Simultaneously by the attraction of the first positively charged metal bar 13 and the repulsion of electronegative second metal bar 14, to which bacterium is from bacterium
Filter membrane 12 is transferred on the first metal bar 13.Distance at initial position B and between stop position A is S, MCU controls system
The rotating speed of system control first motor 3, it is V to make the speed that the first metal bar 13 and the second metal bar 14 are moved to the left, and speed is slower,
So the first metal bar 13 and the first metal bar 14 are from initial position B to the time of stop position At 1=S/V。
When the timer internal of MCU control systems reaches timingt 1Afterwards, i.e. the first metal bar 13 and the second metal bar 14 arrive
After at up to stop position A, the work of controlled level telecontrol equipment makes first motor 3 invert, and collection collarium lifting gear does not work, i.e., and the
Two motors 16 still do not work, the first metal bar 13, the second metal bar 14, collection bacterium becket 24, the band for collecting bacterium metal basin 25
Electrically constant, the timer internal of MCU control systems is still periodicallyt 1, and restart timing.First motor 3 drives the first gold medal
It moves right belonging to stick 13 and the second metal bar 14 from stop position A, and ensures rotating speed and rotating forward that first motor 3 inverts
When rotating speed it is identical, it is also V to make the speed that the first metal bar 13 and the second metal bar 14 move right, and is made on bacteriological filter film 12
Bacterium is shifted more on the first metal bar 13.
The timer internal of MCU control systems reaches timingt 1Afterwards, i.e. the first metal bar 13 and the second metal bar 14 reach
After at initial position B, the work of controlled level telecontrol equipment makes first motor 3 rotate forward, the second motor 16 is still motionless, the first gold medal
It is constant to belong to stick 13, the second metal bar 14, collection bacterium becket 24, the charging property of collection bacterium metal basin 25, the inside of MCU control systems
Timer is still periodicallyt 1, and restart timing.First motor 3 drives the first metal bar 13 and the second metal bar 14 from initial
It is moved to the left at the A of position, and it is V to ensure speed still.So the first metal bar 13 and the second metal bar 14 are controlled in multiple times back and forth
Movement is to be able to more fully collect the bacterium on bacteriological filter film 12.
After fully gathering bacterium, when the first metal bar 13 and the second metal bar 14 reach at stop position A, MCU
Control system controlled level telecontrol equipment is stopped, and first motor 3 stops operating, the first metal bar 13 and the second metal bar 14
It is parked at stop position A, at this point, bacterium is transferred to from the side of bacteriological filter film 12 on the first metal bar 13, it is, carefully
The state that bacterium is dispersed in bacteriological filter film 12 from large area is transferred on the first metal bar 13.
The second metal bar 14 of control of circuit controller 15 is not charged, but the first metal bar 13 is negatively charged, collection bacterium becket 24
Positively charged, collection bacterium metal basin 25 is not charged.At this point, the second nut and 20 sum aggregate bacterium becket 14 of nut seat are in initial position
At D.15 domination set collarium lifting gear of circuit controller works, and the second motor 16 is made to rotate forward, the second nut and 20, nut to
Upper movement, controlled level telecontrol equipment do not work, i.e., first motor 3 does not work, and the timer internal timing of MCU control systems ist 2, and restart timing.Second motor 16 drives collection bacterium becket 24 to be moved upwards since initial position D, until mobile
To at upper limit position C.In motion process, 24 coaxial empty set of collection bacterium becket is outside the first metal bar 13, along the first metal bar 13
Upward vertical movement, referring to Fig. 5.According to the charge principle that identical charges repel each other, there is a natural attraction between the sexes, itself bacterium with faint negative electricity can be same
When by positively charged collection bacterium becket 24 attraction and electronegative first metal bar 13 repulsion, to which bacterium is from the first gold medal
Belong to stick 13 to be transferred on collection bacterium becket 24.Distance at initial position D and between upper limit position C is X, MCU control systems
The rotating speed for controlling the second motor 16 makes to integrate speed that bacterium becket 24 moves upwards also as V, then collection bacterium becket 24 is from initial
The time moved at the D of position at upper limit position C ist 2=X/V。
The timer internal of MCU control systems reaches timingt 2Afterwards, i.e., after collection bacterium becket 24 reaches at upper limit position C,
Domination set collarium lifting gear works, and the second motor 16 reversion, horizontal movement device does not work, and first motor 3 is motionless, the first gold medal
It is constant to belong to stick 13, the second metal bar 14, collection bacterium becket 24, the charging property of collection bacterium metal basin 25, the inside of MCU control systems
Timer timing bet 2, and restart timing.Second motor 16 drives collection bacterium becket 24 since upper limit position C to moving down
Dynamic, it is V to ensure movement speed still, at this point, the first metal bar 13 is excluding bacterium, collection bacterium becket 24 is in absorption bacterium.In MCU
The timer internal of control system reaches timingt 2Afterwards, i.e., after collection bacterium becket 24 reaches initial position D, MCU control system controls
It is not charged to make the first metal bar 13, the second metal bar 14, collection bacterium becket 24 is negatively charged, and collection bacterium metal basin 25 is positively charged.This
When, bacterium is transferred on the smaller collection bacterium becket 24 of area from the first larger metal bar 13 of area, so bacterium
It is distributed more dense.MCU control systems control the reversion of the second motor 16, and first motor 3 is still motionless, the inside of MCU control systems
Timer timing bet 3, and restart timing.Second motor 16 drives collection bacterium becket 24 from initial position D to moving down
Dynamic, it is V to ensure movement speed still, until reaching at the E of lower position.Distance at initial position D and between the E of lower position is
Y, then the time that collection bacterium becket 24 is moved to from initial position D at the E of lower position bet 3=Y/V.In motion process, root
According to the charge principle that identical charges repel each other, there is a natural attraction between the sexes, itself bacterium with faint negative electricity can be simultaneously by positively charged collection bacterium metal
The repulsion of the attraction of basin 25 and electronegative collection bacterium becket 24, and since collection bacterium becket 24 is in the sterile of metal basin 25
It is moved downward in water, makes to fall on bacterium collecting becket 24 in the sterile water of collection bacterium metal basin 25.
The timer internal of MCU control systems reaches timingt 3Afterwards, i.e., after collection bacterium becket 24 reaches at the E of lower position,
Domination set collarium lifting gear works, and the second motor 16 rotates forward, and horizontal movement device does not work, and first motor 3 is motionless, the first gold medal
It is constant to belong to stick 13, the second metal bar 14, collection bacterium becket 24, the charging property of collection bacterium metal basin 25, the inside of MCU control systems
Timer timing bet 3, and restart timing.Second motor 16 drives collection bacterium becket 24 to be transported upwards since the E of lower position
It is dynamic, and ensure that speed is always V.
Timing is reached in the timer internal of MCU control systemst 3Afterwards, i.e., collection bacterium becket 24 reaches at initial position D
Afterwards, MCU control systems control 16 return motion of the second motor, first motor 3 is motionless, the first metal bar 13, the second metal bar 14,
It is constant to collect bacterium becket 24, the charging property of collection bacterium metal basin 25, the timer internal of MCU control systems is periodicallyt 3, lay equal stress on
It is new to start timing.Second motor 16 drives collection bacterium becket 24 to be moved downward since initial position D, and ensures that speed is always
V。
The timer internal of MCU control systems reaches timingt 3Afterwards, i.e., after collection bacterium becket 24 reaches at initial position E,
MCU control system domination set collarium lifting gears work, and 16 forward rotating movements of the second motor, horizontal movement device does not work, and first
Motor 3 is motionless, and the first metal bar 13, the second metal bar 14, collection bacterium becket 24, the charging property of collection bacterium metal basin 25 are constant,
MCU control systems timer internal timing bet 3, and restart timing.Second motor 16 drives collection bacterium becket 24 from position
It sets E to start to move upwards, and ensures that speed is always V.
The timer internal of MCU control systems reaches timingt 3Afterwards, i.e., after collection bacterium becket 24 reaches at initial position D,
MCU control systems control 16 stop motion of the second motor, and domination set bacterium becket 24 is not charged, collection bacterium metal basin 25 without
Electricity.At this point, by repeatedly moving up and down, bacterium has dropped to the sterile water of collection bacterium metal basin 25 from collection bacterium becket 24
In, realize that less sterile water collects the purpose of more bacterium.Then, MCU control systems control first motor 3 inverts, the
Two motors 16 are motionless, and the first metal bar 13, the second metal bar 14, collection bacterium becket 24, collection bacterium metal basin 25 are not charged, MCU
The timer internal of control system is still periodicallyt 1, and restart timing.First motor 3 drives the first metal bar 13 and second
Metal bar 14 from final position A move right, and ensure movement speed be V.
The timer internal of MCU control systems reaches timingt 1Afterwards, i.e. the first metal bar 13 and the second metal bar 14 reach
After at initial position B, MCU control systems control first motor 3 stops operating.At this point, completing collection bacterium, electricity is disconnected by collecting bacterium personnel
Source takes out collection bacterium metal basin 25, carries out subsequent operation.
Claims (6)
1. a kind of charge collection bacterium device, including the fixed flat membrane fixing bracket in a both ends（10）, in film fixing bracket（10）
Intermediate lower section is fixedly connected with a bacteriological filter film（12）, bacteriological filter film（12）Antetheca band bacterium, rear wall without bacterium,
It is characterized in：Bacteriological filter film（12）Front side be equipped with the first metal bar（13）, rear side be equipped with the second metal bar（14）, the first metal
Stick（13）With the second metal bar（14）Upper end be commonly connected to horizontal movement device and by horizontal movement device drive left and right water
Translation is dynamic, and it is initial position to be moved to most right extreme position（B）, most left extreme position be off position（A）, in stop position
It sets（A）First metal bar at place（13）Be arranged right below one collection bacterium becket（24）, collect bacterium becket（24）Underface
Storing has the collection bacterium metal basin for being marked with sterile water（25）；Collect bacterium becket（24）Connection collects collarium lifting gear and by collection collarium
Lifting gear drives to be vertically moved up and down, collects bacterium becket（24）In initial position（D）Place is located at the first metal bar（13）Just
Lower section collects bacterium becket（24）Extreme position on most is upper limit position（C）, most under extreme position be lower position（E）、
Lower position（E）The collection bacterium becket at place（24）It is immersed in collection bacterium metal basin（25）In sterile water；Horizontal movement device sum aggregate bacterium
Ring lifting gear is by circuit controller（15）Control work, circuit controller（15）It is supplied by a MCU control system, a power supply
Electric system, a 5V DC power supply and four switching group compositions, MCU control systems are separately connected electric supply system and four are opened
The positive and negative anodes of pass group, 5V DC power supplies connect four switching group input terminals, the output end of first switch group and the first metal bar（13）
It is connected, the output end of second switch group and the second metal bar（14）It is connected, output end and the collection bacterium becket of third switching group
（24）It is connected, output end and the collection bacterium metal basin of the 4th switching group（25）It is connected.
2. charge collection bacterium device according to claim 1, it is characterized in that：The horizontal movement device fixation includes one first
Motor（3）, two the first guide rails（6）, first lead screw shaft（7）With first nut and nut seat（8）, the first lead screw shaft
（7）, the first guide rail（6）And first motor（3）Output shaft it is horizontally disposed, first motor（3）Output shaft it is coaxially connected
First lead screw shaft（7）, two guide rails（6）Relative to the first lead screw shaft（7）It is front and back to be arranged symmetrically, the first lead screw shaft（7）It is upper coaxial
Cooperation is equipped with the first nut and nut seat（8）, the first nut and nut seat（8）Lower section is fixedly connected with the first metal bar jointly（13）
With the second metal bar（14）Upper end, first motor（3）Circuit controller is connected through control line（15）.
3. charge collection bacterium device according to claim 1, it is characterized in that：The collection collarium lifting gear includes one second electricity
Machine（16）, two the second guide rails（22）, second lead screw shaft（19）With second nut and nut seat（20）, the second leading screw
Axis（19）, the second guide rail（22）With the second motor（16）Output shaft be arranged vertically, the second motor（16）Output shaft it is coaxial
Connect the second lead screw shaft（19）, two second guide rails（22）Relative to the second lead screw shaft（19）It is front and back to be arranged symmetrically, at second
Thick stick axis（19）Upper coaxial cooperation is equipped with the second nut and nut seat（20）, the second nut and nut seat（20）Through collecting collarium fixed frame
（23）It is fixedly connected with collection bacterium becket（24）, the second motor（16）Circuit controller is connected through control line（15）.
4. charge collection bacterium device according to claim 1, it is characterized in that：First metal bar（13）With the second metal bar（14）Phase
For film fixing bracket（10）With bacteriological filter film（12）Front and back to be arranged symmetrically, two metal bars are that rectangle is Metal Flake,
Sheet metal face bacteriological filter film（12）.
5. charge collection bacterium device according to claim 1, it is characterized in that：Collect bacterium metal basin（25）Radius be more than collection bacterium gold
Belong to ring（24）Radius, collect bacterium metal basin（25）Vertical height be to collect 3~4 times of bacterium becket vertical height.
6. a kind of collection bacterium method of charge collection bacterium device as described in claim 1, it is characterized in that comprising the steps of：
A, the first metal bar（13）With the second metal bar（14）In initial position（B）Place, circuit controller（15）Control the first gold medal
Belong to stick（13）Positively charged, the second metal bar（14）Negatively charged, collection bacterium becket（24）Sum aggregate bacterium basin（25）It is not charged；
B, collection collarium lifting gear does not work, and horizontal movement device work drives the first metal bar（13）With the second metal bar
（14）It is moved to the left, makes bacteriological filter film（12）On bacterium simultaneously by the first positively charged metal bar（13）Attraction and band
Second metal bar of negative electricity（14）Repulsion, from bacteriological filter film（12）It is transferred to the first metal bar（13）On；
C, the first metal bar（13）With the second metal bar（14）Reach most left stop position（A）When place, horizontal movement device stops
Only work, circuit controller（15）Control the second metal bar（14）Not charged, the first metal bar（13）Negatively charged, collection bacterium becket
（24）Positively charged, collection bacterium metal basin（25）It is not charged；Collect the work of collarium lifting gear, drives collection bacterium becket（24）First by first
Beginning position（D）Place is moved upward to upper limit position（C）Initial position is returned to behind place still further below（D）, collect bacterium becket（24）Coaxially
Empty set is in the first metal bar（13）Outside and along the first metal bar（13）Vertical up-or-down movement, bacterium is from the first metal bar（13）Transfer
To collection bacterium becket（24）On；
D, collect bacterium becket（24）Return to initial position（D）Afterwards, circuit controller（15）Control the first metal bar（13）, the second gold medal
Belong to stick（14）Not charged, collection bacterium becket（24）Negatively charged, collection bacterium metal basin（25）It is positively charged；Collect collarium lifting gear to drive
Collect bacterium becket（24）By initial position（D）It is moved down into lower position（E）Place collects bacterium becket（24）It is completely immersed in collection bacterium
Metal basin（25）Sterile water in, bacterium is from bacterium collecting becket（24）On fall to collection bacterium metal basin（25）It is sterile
In water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610412953.8A CN106047689B (en) | 2016-06-13 | 2016-06-13 | Charge collection bacterium device and method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610412953.8A CN106047689B (en) | 2016-06-13 | 2016-06-13 | Charge collection bacterium device and method |
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| Publication Number | Publication Date |
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| CN106047689A CN106047689A (en) | 2016-10-26 |
| CN106047689B true CN106047689B (en) | 2018-08-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610412953.8A Expired - Fee Related CN106047689B (en) | 2016-06-13 | 2016-06-13 | Charge collection bacterium device and method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1607007A (en) * | 2003-10-14 | 2005-04-20 | 马士科技有限公司 | Device for processing bacterium by electromagnetic wave |
| CN101048845A (en) * | 2004-08-19 | 2007-10-03 | 塞莫费尼根股份有限公司 | Isolating ions in quadrupole ion traps for mass spectrometry |
| WO2007140752A1 (en) * | 2006-06-06 | 2007-12-13 | Eads Deutschland Gmbh | Micromechanical filter for microparticles, in particular for pathogenic bacteria and viruses, and also process for production thereof |
| CN201598287U (en) * | 2010-02-25 | 2010-10-06 | 卢磊 | Bacterium collecting device for laboratory |
| CN203007272U (en) * | 2013-01-10 | 2013-06-19 | 李玉 | Bacterial examination, separation and filtration device |
-
2016
- 2016-06-13 CN CN201610412953.8A patent/CN106047689B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1607007A (en) * | 2003-10-14 | 2005-04-20 | 马士科技有限公司 | Device for processing bacterium by electromagnetic wave |
| CN101048845A (en) * | 2004-08-19 | 2007-10-03 | 塞莫费尼根股份有限公司 | Isolating ions in quadrupole ion traps for mass spectrometry |
| WO2007140752A1 (en) * | 2006-06-06 | 2007-12-13 | Eads Deutschland Gmbh | Micromechanical filter for microparticles, in particular for pathogenic bacteria and viruses, and also process for production thereof |
| CN201598287U (en) * | 2010-02-25 | 2010-10-06 | 卢磊 | Bacterium collecting device for laboratory |
| CN203007272U (en) * | 2013-01-10 | 2013-06-19 | 李玉 | Bacterial examination, separation and filtration device |
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| Publication number | Publication date |
|---|---|
| CN106047689A (en) | 2016-10-26 |
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