CN106047499A - Extraction method for Pinus koraiensis volatile oil, forest air and application thereof - Google Patents
Extraction method for Pinus koraiensis volatile oil, forest air and application thereof Download PDFInfo
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- CN106047499A CN106047499A CN201610325637.7A CN201610325637A CN106047499A CN 106047499 A CN106047499 A CN 106047499A CN 201610325637 A CN201610325637 A CN 201610325637A CN 106047499 A CN106047499 A CN 106047499A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D47/00—Separating dispersed particles from gases, air or vapours by liquid as separating agent
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
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Abstract
The invention provides an extraction method for Pinus koraiensis volatile oil. The extraction method comprises the steps of mixing Pinus koraiensis needles with distilled water, then, carrying out distillation-extraction, carrying out centrifugation, and then, sucking upper-layer volatile oil, wherein the time for distillation-extraction is 1 to 3 hours, and the liquid/material ratio of the Pinus koraiensis needles to the distilled water is 1: (2.5 to 4). According to the extraction method, through optimizing parameters of an extraction process for the Pinus koraiensis volatile oil, the yield, color, odor and volatility of the Pinus koraiensis volatile oil can all be relatively good; and by adding the Pinus koraiensis volatile oil extracted by the extraction method into forest air, the forest air can play roles in better inhibiting bacteria, cleaning fume air and purifying air.
Description
Technical field
The present invention relates to quintessence oil technical field, particularly relate to the extracting method of a kind of Pinus koraiensis volatile oil, a kind of forest air
And application.
Background technology
Quintessence oil (Essential oils) is also referred to as volatile oil (Volatile oils), be present in plant
Class can be able to be volatilized at normal temperatures with vapor distillation, and has the general name of the volatility oily liquids of certain fragrance.
The organic chemicalss such as monoterpene, sesquiterpene and di-terpene in plants essential oil, have sterilization, antiinflammatory, anticancer
With the performance of promotion growth hormone secretion, to promotion bronchus and renal system activity, suppression spirit is impatient, promotion human body is the oldest
Metabolism, regulation spirit, allaying tiredness and disease-resistant keeping fit all have certain effect.Terpenes organic chemicals in quintessence oil, also
Can promote that air ionization produces abundant anion, particularly forest has huge effect to increasing air-anion concentration.
Anion in air reaches finite concentration, is possible not only to regulate the mood of people, makes people feel with fresh air, unfold pleasant, satisfied
Feelings pleased；And more than 30 kind of disease of 7 systems of human body is had suppression, alleviate and auxiliary therapeutic action, especially to human body
Health-care effect becomes apparent from.
Additionally, plants essential oil can also regulate the emotion of people, may be used for aroma-therapeutic.As far back as ancient times, people have opened
Send out and make use of plant volatile.That utilize spice treatment disease the earliest is the physician R M of France's thirties in 20th century
Gattefosse, he the Oleum lavandula angustifolia that stumbles on not only has sterilization and cooling effect to burning wound, and it is distinctive
Soft fragrance may also function as calm effect of calming the nerves.Going through the research of more than ten years, achievement in research is combined into book, book by this physician
Entitled " aromatotherapy " (Aromatherapy).Hereafter, France, Italy and the scientist of Britain with regard to aromatic substance to nerveous system
The aspects such as the effect of system and sleep are studied.In Japan, fragrance physiopsychology has become an important research direction.
At present, plants essential oil is all applied at aspects such as perfume industry, medical treatment, agriculturals and is developed.Plants essential oil
Broad-spectrum and strong bactericidal activity make it be developed into disinfection medicament.Air sanitizer with plants essential oil as raw material
Can apply to hospital, hotel, restaurant, family room and the public place that the crowd is dense sterilization, its effect and Medical ultraviolet
Sterilization, peracetic acid fumigation and steaming method are suitable.Compare other sterilization methods and disinfectant, plants essential oil fragrant odour, to skin and
Mucous membrane irritation is little, has good DEVELOPMENT PROSPECT.
The fruit of Pinus koraiensis, leaf and trunk are respectively provided with the highest value, and wherein the fruit of Pinus koraiensis and leaf are respectively provided with necessarily
Medical value, thus, applicant provide the extracting method of a kind of Pinus koraiensis volatile oil, to Pinus koraiensis volatile oil add to forest
Air also has certain medical value.
Summary of the invention
Present invention solves the technical problem that the extracting method being to provide a kind of Pinus koraiensis volatile oil, provide according to the present invention
The Pinus koraiensis volatile oil that extracting method extracts adds to have to forest air and suppresses antibacterial, cleaning flue dust air and purify air
Effect.
This application provides the extracting method of a kind of Pinus koraiensis volatile oil, including:
After Pinus koraiensis pin is mixed with distilled water, distillation extraction, after being centrifuged, draw upper strata volatile oil；Described distillation extraction
Time is 1～3h, and described Pinus koraiensis pin is 1:(2.5～4 with the liquid ratio of described distilled water).
Preferably, described Pinus koraiensis pin also includes before mixing with distilled water:
By described Pinus koraiensis pin cutting, a length of 0.8～1.2cm after the cut-out of described Pinus koraiensis pin.
Preferably, a length of 1cm after described Pinus koraiensis pin cuts off.
Preferably, the time of described distillation extraction is 2h.
Preferably, described liquid ratio is 1:2.5.
Preferably, described centrifugal rotating speed is 10000～12000rpm, and the described centrifugal time is 10～15min.
Present invention also provides a kind of forest air, the Pinus koraiensis volatilization extracted including the extracting method described in such scheme
Oil.
Preferably, the content of described Pinus koraiensis volatile oil is 20vol%～40vol%.
Present invention also provides the forest air described in such scheme in smog air cleaner, bacteriostatic and air cleaning
In application.
Preferably, described antibacterial includes escherichia coli and staphylococcus aureus.
This application provides the extracting method of a kind of Pinus koraiensis volatile oil, its be Pinus koraiensis pin is mixed with distilled water after, distillation
Extract, after being centrifuged, draw upper strata volatile oil；The time of described distillation extraction is 1～3h, described Pinus koraiensis pin and described distilled water
Liquid ratio is 1:(2.5～4).The application, by the parameter during optimization Pinus koraiensis volatile oil is drawn, makes the product of Pinus koraiensis volatile oil
Rate, color, abnormal smells from the patient and volatility are all preferable, and Pinus koraiensis volatile oil the application extracted adds to forest air, make forest empty
Gas have preferably suppress antibacterial, cleaning smog air with purify air effect.
Accompanying drawing explanation
Fig. 1 is the total ion of GC-MS of the Pinus koraiensis volatile oil in the embodiment of the present invention 3 after Pinus koraiensis volatile oil placement 0d Yu 20d
Flow graph；
Fig. 2 be in the embodiment of the present invention 3 Pinus koraiensis volatile oil place the GC-MS of the Pinus koraiensis volatile oil after 30d Yu 150d always from
Subflow figure；
Fig. 3 is the GC-MS total ion current figure of volatile oil component after pine needle of red pine extracts；
Fig. 4 is 140 DEG C, 170 DEG C, 180 DEG C, 186 DEG C, the GC-MS total ion current figure of decompression product of distillation；
Fig. 5 is changes of contents curve chart in Pinus koraiensis Constituents of The Essential Oil distillation at different temperatures；
Fig. 6 is the total ion current figure of the GC-MS analysis of Pinus koraiensis Analysis of Essential Oil Components in forest air.
Detailed description of the invention
In order to be further appreciated by the present invention, below in conjunction with embodiment, the preferred embodiment of the invention is described, but
Should be appreciated that these describe simply as to further illustrate the features and advantages of the present invention rather than to the claims in the present invention
Limit.
The embodiment of the invention discloses the extracting method of a kind of Pinus koraiensis volatile oil, including:
After Pinus koraiensis pin is mixed with distilled water, distillation extraction, after being centrifuged, draw upper strata volatile oil；Described distillation extraction
Time is 1～3h, and described Pinus koraiensis pin is 1:(2.5～4 with the liquid ratio of described distilled water).
This application provides the extracting method of a kind of Pinus koraiensis volatile oil, it is that the Pinus koraiensis using steam distillation to carry out is waved
The extraction of hair oil.
During extracting Pinus koraiensis volatile oil, preferably, the application is first by described Pinus koraiensis pin cutting, by it
It is switched to 0.8～1.2cm, more preferably 1cm.Described Pinus koraiensis pin is preferably 1:(2.5～4 with the liquid ratio of described distilled water), more
It is preferably 1:2.5.The time of described extraction is preferably 1～3h, and more preferably 1～2h, example, the time of described extraction is preferred
For 1h or 2h, most preferably 2h.Described centrifugal rotating speed is preferably 10000～12000rpm, more preferably 12000rpm, described
The centrifugal time is preferably 10～15min, more preferably 10min.
According to the present invention, the extracting method of described Pinus koraiensis volatile oil particularly as follows:
By Pinus koraiensis pin cutting, it is placed in volatile oil determination apparatus, adds distilled water, distillation extraction 1～3h, release volatile oil portion
Point, draw upper strata volatile oil after being centrifuged, obtain Pinus koraiensis volatile oil.
The Pinus koraiensis volatile oil that the application extracts is colourless or light yellow transparent liquid；Water insoluble；It is soluble in methanol, second
Alcohol, acetone, ethyl acetate, chloroform, dichloromethane, petroleum ether, normal hexane, hexamethylene etc.；Its proportion is 0.875；Viscosity: turning
During speed 30rpm, volatile oil viscosity is 211.5mPa S (water: 200mPa S), and when rotating speed 12rpm, volatile oil viscosity is
535mPa S (water: 500mPa S)；Pinus koraiensis volatile oil proportion is less than water, and its viscosity number, close to water, has preferable dispersibility
And mobility.
This application provides a kind of forest air, it includes the Pinus koraiensis volatile oil that such scheme extracts.Described forest air
The content of middle Pinus koraiensis volatile oil is preferably 20vol%～40vol%.
Herein described forest air is other forest airs part the application in addition to Pinus koraiensis volatile oil do not have its source
Particularly limiting, the forest air being well known to those skilled in the art, it can be commercially available prod, it is also possible to according to existing side
The forest air that method is collected.
Present invention also provides the application of described forest air, wave containing Pinus koraiensis due in the forest air that the application provides
Hair oil, therefore, described forest air has preferably application at smog air cleaner, bacteriostatic in air cleaning.
For forest air effect in smog air cleaner, smog air refers in air containing harmful
Flue dust, as contained 6,10,14-trimethyl-2-pentadecanones, 3-methyl nonadecane, 3-hexadecanol, 9-octadecenic acid in air
One or more in amide, 2-amylene-4 acid methyl ester and phthalic acid two (2-ethyl hexyl) ester.The forest that the application provides is empty
Gas has preferable elimination effect to flue dust air.
Bacteriostatic for forest air is applied, and herein described antibacterial is preferably escherichia coli or Staphylococcus aureus
Bacterium.
For the application in the air cleaning of forest air, the herein described forest air containing Pinus koraiensis volatile oil is to sky
PM10, PM2.5 in gas, has preferable removal effect.
In order to be further appreciated by the present invention, below in conjunction with the extraction side of the Pinus koraiensis volatile oil that the present invention is provided by embodiment
Method, forest air are described in detail with the application of forest air, and protection scope of the present invention is not limited by the following examples.
Embodiment 1 steam distillation
By Pinus koraiensis pin cutting, taking 200g and put in volatile oil determination apparatus, add distilled water 500ml, distillation extraction 3h, releasing is waved
Hair oil part, 12,000rpm are centrifuged 10min, and absorption upper strata volatile oil in new pipe, weighs weight, calculates volatile oil productivity, note
Record volatile oil color and abnormal smells from the patient etc., taking 5ul volatile oil drops on qualitative filter paper, and room temperature places 5min, records its volatility.
Comparative example 1 solvent extraction method
By Pinus koraiensis pin cutting, take 200g and put in distilling flask, add petroleum ether (boiling range 30-60 DEG C) or ether 500ml, water
Bath reflux, extract, 2h, after filtration, decompression boils off solvent, obtains volatile oil；Weigh weight, calculate volatile oil productivity, record volatilization
Oil color and abnormal smells from the patient etc., take 5ul volatile oil and drop on qualitative filter paper, and room temperature places 5min, records its volatility.Table 1 is for implementing
Example 1 uses the Pinus koraiensis volatile oil experimental result data table that different extracting method extracts from comparative example 1.
Table 1 Different Extraction Method affects tables of data to Pinus koraiensis volatile oil
As shown in Table 1, the volatile oil productivity that steam distillation extracts is relatively low, and color is shallower, has preferable fragrant, waves
After sending out, residue is few；Although and high with solvent extraction volatile oil productivity, but volatile oil color is deep, and Oleum Pini taste is the densest, and waves
After sending out, oil mark is obvious, has more residue.In actual applications, volatile oil should be with its volatility, and the fragrant composition of tax is attached most importance to and examined
Consider key element, therefore be proposed with steam distillation and extract Pinus koraiensis volatile oil.
Embodiment 2 steam distillation extracts Pinus koraiensis volatile oil process optimization
(1) extraction time optimizes
By Pinus koraiensis pin cutting, take 200g and put in volatile oil determination apparatus, add distilled water 500ml, extract 1 respectively, 2,3h, take
Volatile oil part, 12,000rpm are centrifuged weighing weight after 10min, calculate volatile oil productivity, record volatile oil and abnormal smells from the patient etc., take
5ul volatile oil drops on qualitative filter paper, and room temperature places 5min, records its volatility, and experimental result is as shown in table 2.
The tables of data that Pinus koraiensis volatile oil is affected by table 2 extraction time
Test result indicate that, extract 1, the Pinus koraiensis volatile oil of 2h almost colourless, also without being clearly distinguished from character, abnormal smells from the patient,
But latter yield is higher, the volatile oil extracting 3h is light yellow, Oleum Pini taste also compared with 1, the volatile oil of 2h denser, volatile oil productivity
About 3% is improved compared with during 2h.Take the Pinus koraiensis volatile oil of different extraction time, analyze its chemical composition difference, result through GC-MS
Show, extract 1, the Pinus koraiensis volatile oil chemical composition of 2h close, but compared with extracting 3h, the above two low boiling components are more, and
The high boiling component of 3h is more, i.e. its difficult more (concrete data show pine Constituents of The Essential Oil analysis portion of volatile component content
Point).Based on the above results, determine that Pinus koraiensis volatile oil is drawn time 2h is advisable.
(2) optimization of solid-liquid ratio condition
By Pinus koraiensis pin cutting, by solid-liquid ratio 1:4,1:3,1:2.5,1:2 put in volatile oil determination apparatus, and distillation extraction 2h takes
Volatile oil part, 12,000rpm are centrifuged weighing weight after 10min, calculate volatile oil productivity, and experimental result is as shown in table 3,
Table 3 solid-liquid ratio affects tables of data to Pinus koraiensis volatile oil is drawn
Test result indicate that, solid-liquid ratio is between 1:2.5-4, and Pinus koraiensis volatile oil productivity difference is little, produces less than during 1:2.5
Rate declines more apparent.1:2.5 is suitable solid-liquid ratio.
(3) optimization of raw material breaking method
By Pinus koraiensis pin cutting, add distilled water by solid-liquid ratio 1:2.5, be crushed to without obvious stripping and slicing in tissue pulverizer,
Proceed in volatile oil determination apparatus, distillation extraction 2h, does the volatile oil is drawn test of Pinus koraiensis pin cutting simultaneously, extracts and will wave after terminating
Hair oil part is in 12, and 000rpm is centrifuged 10min, weighs volatile oil weight, calculates productivity, records volatile oil and abnormal smells from the patient etc., takes 5ul
Volatile oil drops on qualitative filter paper, and room temperature places 5min, records its volatility, and experimental result is as shown in table 4.
The different material handling procedures impact on Pinus koraiensis volatile oil is drawn of table 4
Test result indicate that, after being pulverized by Folium Pini, its volatile oil yield increased, color burn, and abnormal smells from the patient is the densest,
Abnormal smells from the patient after boiling with plant；From olfactory organoleptic preferably the volatile oil that cutting is extracted.In sum, it is contemplated that productivity,
The factor such as character, abnormal smells from the patient, determines that Pinus koraiensis Extraction Process of Volatile Oil is as follows: Pinus koraiensis volatile oil is drawn uses steam distillation,
Ask Folium Pini cutting (about 1cm), add distilled water, distillation time 2h by solid-liquid ratio 1:2.5.
Embodiment 3 Pinus koraiensis stability of volatile oil research
Volatile oil is generally in room temperature preservation, and containing antibacterial composition, general storage life is longer due to wherein many, but between its component or
Composition may be caused to change with air Long contact time.
For examining or check the stability of Pinus koraiensis volatile oil compositions, after volatile oil anhydrous sodium sulfate dehydration, under the conditions of 37 DEG C
After placing 0d, 5d, 10d, 20d, 30d, 50d and 150d, take volatile oil, analyze its chemical composition with GC-MS.0d, 20d Pinus koraiensis is waved
Hair oil GC-MS analyze total ion current figure as it is shown in figure 1, in Fig. 1 black spectral line be that volatile oil is in total ion of 37 DEG C of incubation 0d
Flow graph, pink colour is incubation 20d, and lower section is that part is amplified；The total ion current figure that 30d, 150d Pinus koraiensis volatile oil GC-MS analyzes such as figure
Shown in 2.In Fig. 2 black spectral line be volatile oil in the total ion current figure of 37 DEG C of incubation 30d, pink colour is incubation 150d, and lower section is portion
Divide and amplify.
GC-MS analysis result shows, Pinus koraiensis volatile oil 37 DEG C of incubations 150 days, its Main Ingredients and Appearance compared with before incubation also
Significant change, prompting Pinus koraiensis volatile oil is not occurred to have fabulous stability, can preserve in the room temperature long period.
The GC-MS of embodiment 4 Pinus koraiensis volatile oil analyzes
(1) GC-MS analyze sample process: GC-MS analyze require that sample size is the lowest, volatile oil can not direct injected, need
Could be used for after dilution analyzing；In this research, volatile oil sample standard deviation is diluted to 10% (V/V) through normal hexane, is sufficiently mixed, put from
In scheming, it is centrifuged 5min in 12000rpm, takes supernatant 1 μ L sample introduction.
(2) GC-MS analysis condition:
1. analytical tool: Shimadzu Corporation's gas chromatograph-mass spectrometer: GCMS-QP2010Ultra, automatic sampler: AOC-20i+s；
2. GC condition: with helium as carrier gas, Rtx-5MS capillary chromatographic column (30m*0.25 μm * 0.25mm), injection port temperature
Spend T=250 DEG C, sample size 1 μ L, split ratio 100:1, flow velocity 1.0mL/min；Temperature programming 8 DEG C/min to 200 DEG C, keeps
2min；10 DEG C/min is warming up to 280 DEG C again, keeps 5.5min；
3. MS condition: ion source temperature T=200 DEG C, interface temperature T=250 DEG C, solvent extends time 3.5min, detection
Voltage 0.2kV, time started 3.5min, end time 33min, be spaced 0.10s, scanning speed 1000, sweep limits: m/z
35-500；
4. data parsing: mass spectral results is retrieved in GCMS-QP2010Ultra mass spectrometric data storehouse NIST11, after through people
Compound corresponding to component each in oil degradation product is identified by work correction.
(3) Pinus koraiensis Constituents of The Essential Oil
Extracting Korean Needle section 2h with steam distillation, the GC-MS result of obtained volatile oil is as shown in table 5；Composition
Analysis result shows, containing about 50 kinds compounds in Pinus koraiensis volatile oil, wherein comprises and numerous composes fragrant composition, as ocimene, β-
Pinene, beta-myrcene, D-limonene, terpinene-4-alcohol, α-terpinol, terpinenyl aceatae, ylangene, caryophyllene, west pine alkene etc.,
Also having some is the peculiar fragrance ingredient of pine genus plant, such as: carene, terpinene, terpinolene, terpinenyl aceatae, longipinene etc..
Some component also has unique effect, as phellandrene has insecticidal action；D-limonene has antitumaous effect, it is possible to suppression cholesterol
The effects such as synthesis；Humulene has anti-inflammatory efficacy, has the respiratory tract disease virus functions such as suppression flu, pneumonia；α-bisabolol tool
There is antiinflammatory, antibacterial, suppression digestive enzyme effect etc..
Table 5 Pinus koraiensis volatile oil GC-MS analysis result data table
The GC-MS Comparative of the different extraction time Pinus koraiensis volatile oil of embodiment 5 analyzes
Pine needle of red pine extracts 1h, 2h and 3h through steam distillation, GC-MS total ion current figure such as Fig. 3 of Constituents of The Essential Oil
Shown in.As seen from the figure, extract 1,2h Constituents of The Essential Oil the most close, extract 3h main composition generation significant change, it may be possible to its face
Color is relatively deep, the reason that abnormal smells from the patient is dense.The volatile oil key component of different extraction times is shown in Table 6, analysis of components result and total ion
Flow graph result matches, extract 1,2h gained Constituents of The Essential Oil the most close, extract 3h volatile oil low boiling component relative amount
The volatile oil extracted significantly lower than the short time, its main composition isopulegol is the new component not occurred in short time extract,
Another Main Ingredients and Appearance o-cymene content is apparently higher than short time extract.It addition, the long-time volatile oil extracted also has pine
Carvone, myrthenal etc. extract the component not available for volatile oil in the short time.In a word, Pinus koraiensis during long-time extraction
Volatile oil compositions generation significant change, should be noted that in actual volatile oil preparation process.
The different extraction time Pinus koraiensis volatile oil compositions analytical data of table 6
The GC-MS of embodiment 6 Pinus koraiensis volatile oil fractional distillation product analyzes
If the Pinus koraiensis volatile oil extracted is for forest air Related product, it is desirable to volatile oil has preferable dispersibility and waves
The property sent out.And some high boiling components contained in Pinus koraiensis are the most volatile, on the other hand volatile oil viscosity may be caused to increase
Add, be unfavorable for the aerial volatilization of volatile oil and uniformly diffusion.This project uses rectification method, by Pinus koraiensis volatile oil compositions by it
Boiling point difference is separated, and by the component analysis to different temperatures section product of distillation, determines suitable Pinus koraiensis oleopene
Goods, have been allowed to more pleasant odor, are more beneficial for adding in forest air product.
The Pinus koraiensis volatile oil about 50ml that water intaking vapor distillation method extracts, puts in 100ml distilling apparatus, at still head turning
Placing thermometer, oil bath is heated to producing volatile oil steam, collects distillation, and recording thermometer temperature, raises oil bath temperature,
Make different boiling component distillate successively, finally with decompression distillation, residual volatile components is steamed.Each temperature section distillation is used
After normal hexane dilutes 10 times, take 1ul and carry out GC-MS analysis.Sample treatment and analysis condition are with embodiment 4.
140 DEG C, 170 DEG C, 180 DEG C, 186 DEG C, decompression product of distillation GC-MS total ion current figure as shown in Figure 4, in Fig. 4
Black, pink colour, blueness, brown, green spectral line respectively 140 DEG C, 170 DEG C, 180 DEG C, 186 DEG C, the Pinus koraiensis that distills out of decompression waves
Hair oil, figure (A)～figure (E) are the amplification of different retention times in total ion current figure.Result shows, to protect in distillation during low temperature
The product staying the time short is main, and as under the conditions of 140 DEG C, component focuses primarily upon between 5-8min, and retention time is more than 10min
Product few；And under the high temperature conditions, under the conditions of 186 DEG C, component retention time focuses primarily upon 10-17min, during reservation
Between short component less, and multiple high boiling component can't detect when low temperature.
140 DEG C, 170 DEG C, 180 DEG C, 186 DEG C, Partial Characterization result such as table 7 institute of decompression distillation Pinus koraiensis volatile oil compositions
Show.Composition analysis result shows, when low temperature distillation, low boiling component is first distillated, with some unsaturated bands in these fractions
Alkane be main, such as 3-methylene-bicyclo-[4.3.0] nonane (19.68%), three rings [5.3.0.0 (4,8)] decane
(12.56%), 7,7-dimethyl-2-methene-bicyclo-[2.2.1] heptane (7.71%), 1-methyl-3-(1-methyl ethylene)
Hexamethylene (18.20%) etc., also have some aromatics components, such as phellandrene (1.75%) carene (1.35%), o-cymene
(0.75%), γ-terpinene (7.63%) etc., the component that boiling point is higher only has up to borneol acetate (2.17%).When temperature liter
When height is to about 170 DEG C, the aroma ingredients that some boiling points are higher starts to distillate, such as Herba Menthae-4,8-diene (9.60%), camphanone
(1.00%), isoborneol (2.04%), α-terpinene (0.74%), caryophyllene (3.03%) etc..When temperature is increased to 180 DEG C,
The basic noresidue of low boiling component, more high boiling point aromatised fraction distillates, such as α-cubebene (1.22%), copaene
(2.26%), β-ylangene (2.83%), alloaromadendrene (0.8%), γ-muurolene (4.46%), T-cadinol
(1.66%), α-cadinol (0.96%) etc..Because more fraction can not be obtained by the method improving temperature, we take
The volatile component that volatile oil is remaining is distilled by the mode of decompression distillation further, and in this part fraction, retention time exists
The constituent content of below 10min is less, high boiling component showed increased (more than retention time 13min), also has some by adding
The component that can not distillate of heat, as clear in oxalene (1.15%), guaiene (6.43%), β-Vatirenene (13.03%), Bi
Eggplant oil enol (0.41%) etc..
Notice that some components all existed in each stage simultaneously, such as o-cymene, relative amount between 0.7-1.4%,
Borneol acetate relative amount is between 2-9%, and 4-carene is between 0.4-1.9% etc..Part Pinus koraiensis Constituents of The Essential Oil is not
In synthermal lower distillation, changes of contents situation embodies in Figure 5 in Fig. 5 ◆ curve is 3-methylene-bicyclo-[4.3.0] nonane
Changes of contents curve, ■ curve is the changes of contents curve of α-phellandrene, and ▲ curve is the changes of contents curve of 4-carene,
Curve is the changes of contents curve chart of borneol acetate, and ◇ is the changes of contents curve of caryophyllene, ● for the content of α-cadinol
Change curve.The trend of the short component 3-methylene of retention time-bicyclo-[4.3.0] nonane, α-phellandrene, 4-carene etc. is
It is gradually lowered, is wholly absent in high-temperature part；The component of retention time length can not distillate under cryogenic, along with temperature raises
Quantity of distillate increases, such as cadinol.It is in the part of centre, then presents and first raise the trend reduced afterwards, such as borneol acetate and stone
Bamboo etc..
In sum, low boiling and high boiling component, through classification fractional distillation, can substantially be separated, thus obtain by Pinus koraiensis volatile oil
Obtaining low boiling component or the fraction of high boiling component concentration, the research and development for the refined of follow-up Pinus koraiensis volatile oil and distinguishing products provide
Article one, good approach.
The different vapo(u)rizing temperature Pinus koraiensis volatile oil compositions analytical data of table 7
In embodiment 7 forest air product, Pinus koraiensis Constituents of The Essential Oil is analyzed
The Pinus koraiensis volatile oil extracted through steam distillation is added in forest air product, after standing 30 days, release
Forest air, absorbs through normal hexane, and application GC-MS method analyzes wherein volatile ingredient.The total ion current figure that GC-MS analyzes such as figure
Shown in 6, it can be seen that after Pinus koraiensis volatile oil is added to forest air and again discharges, lose most of high boiling point group
Point, the most volatile low boiling component can make forest air possess the delicate fragrance of pinaster by release.
The Constituents of The Essential Oil analysis result of forest air release is shown in Table 8, test result indicate that, Pinus koraiensis volatile oil adds forest
After air again release, wherein volatile component is mainly australene (48.62%), is secondly camphene (10.82%), D-ramie
Alkene (9%), nopinene (8.78%), beta-myrcene (7.76%) etc..
The GC-MS analytical data that table 8 Pinus koraiensis volatile oil discharges in forest air
The GC-MS of smog air cleaning effect is analyzed by embodiment 8 forest air
The anion contained in forest air etc. have stronger removal effect to flue dust.For investigating containing Pinus koraiensis volatile oil
Smoke abatement effect after forest air release, relative analysis is with or without the volatile component of forest air release smoking room.Concrete side
Method is as follows:
2 area about 10m are set2, structure, the identical room such as interior jewelry, burn 2 medicated cigarettes with identical speed, one
Room discharges forest air with 20L/min speed, and another room discharges normal air with identical speed.After 20min, two rooms
Between start simultaneously air filtration absorption (with normal hexane as lyosoption), soak time 20min.Take the normal hexane after absorption molten
Liquid, is settled to 10ml, takes 2ul and carries out GC-MS analysis, instrument and analysis condition with embodiment 4.
GC-MS analysis result is shown in Table 9, and analysis result shows, does not adds in the air of normal air group and contains in a large number human body
Harmful or irritant composition, such as 6,10,14-trimethyl-2-pentadecanones, 3-methyl nonadecane, 3-hexadecanol, 9-18
Enoic, 2-amylene-4 acid methyl ester, phthalic acid two (2-ethyl hexyl) ester etc., and forest air release group fails to detect this
Class material, illustrates that noxious fume etc. in air can be effectively removed in the release of forest air, has excellent air cleaner effect.
Table 9 forest air is to the GC-MS analytical data of flue dust elimination effect in air
Embodiment 9 Pinus koraiensis quintessence oil is to common bacteria inhibitory action research in air
The Pinus koraiensis quintessence oil that Pinus koraiensis extraction of essential oil selects distinct methods to extract is used for bacteriostatic experiment, is respectively as follows: directly purchase
Pinus koraiensis quintessence oil, the Pinus koraiensis quintessence oil extracted after crushed, Pinus koraiensis quintessence oil 3 class of the most size-reduced extraction.
Bacterial species bacterial species escherichia coli (Escherichia coli), staphylococcus aureus
(Staphylococcus aureus), bacillus subtilis (Bacillus subtilis) are antibacterial kinds most commonly seen in air
Class, wherein staphylococcus aureus is ubiquitous in nature, all may be used in the Excreta of empty gas and water, dust and humans and animals
Finding, the Center for Disease Control is reported, staphylococcus aureus (gram positive bacteria Typical Representative) infection caused accounts for
Second, is only second to escherichia coli (gram negative bacteria Typical Representative).Additionally, compare from experiment toleration, hay resistance to
Stress is more than gold Portugal, and gold Portugal is more than large intestine, therefore selects these 3 kinds of antibacterials to supply examination strain as what this was studied.
After 3 kinds are activated by bacteria suspension configuration for examination bacterial solids slant medium, it is then transferred to nutrient broth liquid
Culture medium is cultivated further, then utilizes plating medium that each bacterial concentration is transferred to 5 × 105CFU is standby.
Bacteriostasis measures Pinus koraiensis quintessence oil Tween-80 and dilutes, and filters through 0.22 μm microfilter, draws diluent,
Differently configured concentration in nutrient broth, is respectively as follows: 1 μ L/ml, 5 μ L/ml, 10 μ L/ml, 20 μ Ll/ml, 50 μ L/ml.Will preparation
Good each strain bacteria suspension takes 5ml respectively and is added in test tube, and constant-temperature shaking culture at 37 DEG C, 24h carries out observed and recorded.Each dense
Degree gradient arranges 3 and repeats and 1 blank.Blank is not added with any quintessence oil series products.
With bacteriostasis rate as testing index.
Cultivate test tube explanation the most muddy after terminating and have bacteria growing, transparent test tube, blank pipe are sought
Supporting the smearing culture counting of flat board, each nutrition flat board drips 100 μ L and treats that test agent carries out cultivating and observe, and each treats test agent weight
Multiple 3 times.
The qualitative bacteriostatic experiment result of Pinus koraiensis quintessence oil is as shown in table 10, table 10 show, 3 class Pinus koraiensis quintessence oils are to large intestine bar
Bacterium, staphylococcus aureus all have obvious biocidal property, to bacillus subtilis unrestraint effect.
Table 10 Pinus koraiensis quintessence oil is to bacteriostasis tables of data
Analytical table 10 understands, and the Pinus koraiensis quintessence oil of purchase starts to show under 5 μ l/ml concentration and makees colibacillary suppression
With, along with the increase of essential oil concentration, bacteriostasis rate is gradually increased, and under 50 μ l/ml concentration, can completely inhibit Escherichia coli Growth.
The Pinus koraiensis quintessence oil of 1-5 μ l/ml concentration does not shows inhibitory action to staphylococcus aureus, but when essential oil concentration is higher than 10 μ l/ml
Time, bacteriostasis rate reaches 100%.
The Pinus koraiensis quintessence oil extracted after crushed does not demonstrates in 1-10 μ l/ml concentration range to be made colibacillary suppression
With, along with the increase of essential oil concentration, inhibitory action strengthens, and colibacillary bacteriostasis rate is reached by 20 μ l/ml essential oil concentration
40.80%, 50 μ l/ml essential oil concentration reach 76.32% to colibacillary bacteriostasis rate.The Pinus koraiensis quintessence oil pair of 1-5 μ l/ml concentration
Staphylococcus aureus does not shows inhibitory action, but as essential oil concentration 10 μ l/ml, bacteriostasis rate reaches 71.63%, and along with essence
The increase of oil concentration, bacteriostasis rate strengthens.
The Pinus koraiensis quintessence oil of the most size-reduced extraction does not demonstrates inhibitory action to escherichia coli, at 10-in 1-5 μ l/ml concentration
In 50 μ l/ml concentration ranges, bacteriostasis rate increases with drug concentration and strengthens, and during essential oil concentration 50 μ l/ml, bacteriostasis rate is 84.21%.
Quintessence oil does not shows inhibitory action in 1-10 μ l/ml concentration range to staphylococcus aureus, but as essential oil concentration 20 μ l/ml
Time, bacteriostasis rate reaches 71.63%, and during essential oil concentration 50 μ l/ml, bacteriostasis rate is 91.82%, indicates the increase along with essential oil concentration
Bacteriostasis rate strengthens.
Therefore, in the range of the essential oil concentration that experiment sets, 3 class Pinus koraiensis quintessence oils are to escherichia coli, staphylococcus aureus
Rejection ability all along with essential oil concentration increase bacteriostasis rate strengthen.
Embodiment 10 contains quintessence oil forest air to bacterial number inhibitory action research
The culture dish of cut-off footpath 9cm, injects nutrient agar on superclean bench, uses after condensation.In sampling
5, Nutrient agar culture dish is respectively placed in center, room and four angular direction, opens plate, is exposed to 10min in air, then will training
Support ware to cultivate as 37 DEG C of constant incubators, after 24h, observe counting.Release forest air (containing Pinus koraiensis volatile oil), after 30min
Sub-sampling again.According to formula formula difficult to understand calculate every cubic metre contained by bacterial population.
At the abundant burning cigarette in sampling room, form smoky environment, sample in room with reference to above method.Release after sampling
Put forest air, sub-sampling again after 30min.According to formula formula difficult to understand calculate every cubic metre contained by bacterial population.
Bacterial population/m3(CFU/m3)=50000N/AT；
A: platen area (cm2)；T: the plate exposure time (min)；N: average colony number (CFU)
Result is as shown in table 11, as shown in Table 11, before and after release is containing the forest air of Pinus koraiensis quintessence oil, and bacterial population in air
There is notable change in amount.In non smoke environment, containing quintessence oil forest air effect 30min, bacteriostasis rate is up to 31.92%；There is cigarette
Under mist environment, bacteriostasis rate is up to 23.30%.
The bacteriostasis rate of antibacterial is found by relatively two kinds of environment containing quintessence oil forest air, under smoky environment, sterilizing ability
Reduce；Produce the reason of this kind of result to be probably in smoky environment and can not be fully contacted with antibacterial in air containing quintessence oil gas,
In table 11 varying environment, the inhibitory action of bacterial number is compared tables of data by forest air
It follows that add Pinus koraiensis quintessence oil in forest air product, it is effectively reduced bacterial number in air.Quintessence oil class
Product is pure natural plant volatile oil, and low dose of release i.e. has certain bactericidal effect, and easy to use, penetrating odor is little,
And containing materials such as a lot of monoterpene, sesquiterpene and di-terpenes, also there is while playing sterilization, antiinflammatory action regulation essence
God, allaying tiredness, disease-resistant effect such as keep fit.
Air quality monitoring in the compression release of embodiment 11 forest air
Sampled point, sampling environment, height of sampling and the requirement of sample frequency in sampling ambient air monitoring, by " environment
Specifications of Monitoring Technology " (atmospheric portion) execution.
Analysis method every pollutant analysis method, is shown in Table 12.
Table 12 every pollutant analysis method tables of data
Experimental technique chooses 2 area about 10m2, structure, the identical room such as interior jewelry, burn 2 perfume (or spice) with identical speed
Sampling and measuring after cigarette, Monitoring factors chooses NO2、SO2、O3、PM10、PM2.5.Later of having sampled room is released with 20L/min speed
Putting forest air, another room discharges normal air with identical speed, and after 20min, two rooms start atmospheric sampling simultaneously
Device, the above-mentioned factor of sample analysis, sampling time 20min.
1. nitrogen dioxide (NO2)
NO be colourless, tasteless, be slightly soluble in water, be the most easily oxidized to NO2, NO2For brownish red, there is intense stimulus
Property gas.
1) principle: with glacial acetic acid, double aminobenzenesulfonic acids and ethylene diamine hydrochloride are made into absorbing liquid, NO in air2Oxidized
Become HNO3And HNO2.Under conditions of glacial acetic acid exists, nitrous acid and p-aminobenzene sulfonic acid generation diazo-reaction, the most again with
Absorbing liquid coupling, generates rose azo-compound, and its shade is directly proportional to the content in sample, then uses light splitting light
Degree method measures.
2) drafting of standard curve: configuration standard series, respectively adds the colour developing of equivalent absorbing liquid, and constant volume makes standard curve color
Row, measure its absorbance at 540nm respectively, draw standard curve according to data value, the absorbance of mensuration sample solution:
In formula: the absorbance of A-sample solution
A0The absorbance of-blank reagent solution
The slope of b-standard curve regression equation, absorbance ml/ μ g；
The intercept of a-standard curve regression equation；
Absorbing liquid volume, mL are used in V-sampling；
V0-it is scaled the volume under standard state (273K, 101.3kPa), L；
The extension rate of D-sample；
F-saltzman test coefficient, 0.88 (when in air, content of nitrogen dioxide is higher than 0.720mg/m3Time, f value is
0.77)。
3) instrument: absorption bottle, Portable sampling device, spectrophotometer, silica gel tube etc..
4) reagent: N-(1-naphthyl) ethylenediamine-hydrochloride storing solution, absorption stock solution, sampling absorbing liquid, chromic acid, nitrous
Acid sodium, nitrous acid standard reserving solution, sodium nitrite titer.
5) determination step:
The drafting of standard curve: take 6 10ml color-comparison tubes, prepare standard series by table 13.
Table 13 sodium nitrite standard series table
| Guan Hao | 0 | 1 | 2 | 3 | 4 | 5 |
| Sodium nitrite standard solution (2.5 μ g/ml) | 0 | 0.40 | 0.80 | 1.20 | 1.60 | 2.00 |
| Absorb stock solution | 8.00 | 8.00 | 8.00 | 8.00 | 8.00 | 8.00 |
| Water (mL) | 2.00 | 1.60 | 1.20 | 0.80 | 0.40 | 0 |
| Nitrite anions content (μ g/ml) | 0 | 0.10 | 0.20 | 0.30 | 0.40 | 0.50 |
The mensuration of sample: after sampling, places 20min (temperature low adaptation proper extension developing time.During such as room temperature 15 DEG C,
Colour developing more than 40min), with handwashing liquid, the volume of handwashing liquid in sampling bottle is mended to graticule, mixing.Sampling solution is moved into 1cm
In cuvette, the method for standard curve is used to measure test solution blank solution and the absorbance of sample solution.If the suction of sample solution
Luminosity is above standard the determination of the upper limit of curve, and available diluted measures absorbance again.Result of calculation should be multiplied by dilution times
Number.
2. sulfur dioxide (SO2)
1) principle: the SO in gas sample2Tetrachloro hydrargyrum potassium absorbs, and generates stable dichloro sulphite complexes, this complex
React with formaldehyde and Pararosaniline again, raw empurpled complex.Its shade and SO2Concentration is directly proportional, and uses light splitting light
Degree method measures, and reaction equation is as follows:
HgCl2+ 2KCl=K2[Hg(Cl)4]
[HgCl4]2-+SO2+H2O=[HgCl2SO3]2*+2H++2Cl-
[HgCl2SO3]+HCHO+2H=HgCl+HOCH2SO3H (hydroxymethyl sulphuric acid)
2) standard curve: first referred to standard series, with distilled water at maximum absorption wavelength with sodium sulfite standard solution
The absorbance measured for reference, draws standard luminosity with blank reagent correction absorbance content, then surveys by same method
Sample solution after fixed colour developing, after blank solution correction, calculates SO in sample gas by following equation2Content:
In formula: the absorbance of A-sample solution；
A0The absorbance of-blank reagent solution；
BS-correction factor 1/b, 10-3mgSO2/ (absorbance 12mL)；
The slope of b-regression equation；
Vt-sample solution cumulative volume, mL；
VaTaken sample volume during-mensuration, mL；
Vn-sampling volume nominally, L；
3) instrument:
A, absorption tube: porous ripple plate absorption tube, small impingement is absorption tube or larger bubbles absorption tube, arrives for 30 minutes
Sampling in one hour；125 milliliters of porous ripple plate absorption bottles or 125 milliliters of Drexel bottles, for sampling in 24 hours.
B, atmospheric sampling: range of flow 0-1 liter/min.
C, spectrophotometer
4) reagent: water used is the double distilled water removing oxidant.
0.04M tetrachloro hydrargyrum potassium (TCM) absorption tube: weigh 10.9 grams of mercuric chloride (HgCl2), 6.0 grams of potassium chloride (KCl) and
0.06 gram of disodium EDTA (Na2-EDTA) soluble in water, it is diluted to 1 liter.This solution ph is about 4, at acidometer
Upper 0.01N sodium hydroxide solution regulation pH value is to about 5.2, and this reagent can be stablized six months；0.6% sulfamic acid amine is molten
Liquid；0.2% formalin；0.1N iodine stock solution；0.01N iodine solution；Starch indicator；0.1000N potassium iodate standard solution；
0.1N sodium thiosulfate stock solution；0.01N hypo solution；Sulfur dioxide standard solution；Pararosaniline hydrochloride；
0.016% pair of pinkish red use liquid；1N hydrochloric acid solution；3M phosphoric acid solution；1M NaAc_HAc buffer solution.
5) sample determination: absorbing liquid is moved in 25 milliliters of volumetric flasks, rinse absorption tube with about 5 milliliters of water.Measure 24 little
Time mean concentration time, by absorbing liquid, sample volume is adjusted to 75 or 100 milliliters of graticules, absorb 10.00 milliliters of sample solutions in
25 milliliters of volumetric flasks.Sample is placed 20 minutes, in order to ozone decomposed.Reagent blank liquid should be measured with each batch sample and control sample
Product, with reliability and the accuracy of operation of detectable.Collocation method: absorb 10.00 milliliters of tetrachloro hydrargyrum potassium solutions in 25 millis
Rise volumetric flask, be made into reagent blank liquid；Absorb 2.00 milliliters of sulfur dichloride solution (2.0 mcg/ml) in 25 milliliters of volumetric flasks
In, add 8.00 milliliters of tetrachloro hydrargyrum potassium solutions, be made into control sample.At reagent blank liquid, control in sample and whole sample, point
Not Jia Ru 1.00 milliliter of 0.6% sulfamic acid ammonium salt solution, shake up, place ten minutes to go the interference of denitrification, below walk
Suddenly with the drafting of standard curve.
Table 14 standard series table
If temperature when measuring sample and temperature difference when drawing standard curve are less than 2 DEG C, then the two reagent
Blank absorbency difference is not to be exceeded 0.03.As exceeded this value, standard curve should be repainted.If the absorbance of sample is 1.0
～between 2.0, available reagent blank solution dilutes, and measures absorbance in several minutes again, make the absorbance that records 0.03～
Between 1.0, nitrogen dilution multiple is not larger than 6 times.
3. mensuration PM of granule density it is can absorb10
Principle: make the air of certain volume, by with 10-3The big traffic sampling device of mm sickle, less than 10-3Mm's
Pellet is trapped within the filter membrane of constant weight with the outlet of the separated device of air-flow, according to the quality of filter membrane before and after sampling
And sampling volume, inspirable particle concentration, use/m can be calculated3Represent.
(2)PM10Sampler: have 10-3The big traffic sampling device of mm sickle.
Collection efficiency is Particulate Air kinetic diameter D when 50%50=(10 ± 1) 10-3mm.The geometry of cutting curve
Standard deviation δg≤ (1.5 ± 0.1), under windy condition, (wind speed is less than 8m/s) sickle entrance should have isotropism effect.
Monitoring result and analysis: before and after forest air release, air quality measurement result is shown in Table 14, O3Concentration before and after release
Relatively stable, it is maintained at 12 μ g/m3, and NO2、SO2Slightly reduce after release, but overall three's concentration is relatively stable.In particulate matter
PM10In a slight decrease, by 97 μ g/m3It is reduced to 81 μ g/m3, PM2.5By 79 μ g/m3Become 64 μ g/m3, possible reason has: forest
The air (containing the Pinus koraiensis volatile oil) anion containing higher concentration, changes the charged attribute of air particle, destroys gas molten
The stability of glue so that partial particulate thing is attached to the indoor surface of solids, shows as PM in air10、PM2.5Concentration is minimized；
It addition, the membrane retention of gas collecting instrument partial particulate thing so that air particles substrate concentration total number reduces.
Table 15 air pollutants measurement result tables of data
| Project | Before release | After release | Unit |
| NO2 | 58 | 56 | μg/m3 |
| O3 | 12 | 12 | μg/m3 |
| SO2 | 28 | 24 | μg/m3 |
| PM10 | 97 | 81 | μg/m3 |
| PM2.5 | 79 | 64 | μg/m3 |
| Temperature | 28 | 27 | ℃ |
As shown in Table 15, O in air3、NO2、SO2Before and after release, concentration is relatively stable, O3It is maintained at 12 μ g/m3, and NO2、
SO2Slightly reduce after release, but overall three's concentration change is little；Particulate matter PM10In a slight decrease, by 97 μ g/m3It is reduced to 81 μ
g/m3, PM2.5By 79 μ g/m3Become 64 μ g/m3。
The explanation of above example is only intended to help to understand method and the core concept thereof of the present invention.It is right to it should be pointed out that,
For those skilled in the art, under the premise without departing from the principles of the invention, it is also possible to the present invention is carried out
Some improvement and modification, these improve and modify in the protection domain also falling into the claims in the present invention.
Described above to the disclosed embodiments, makes professional and technical personnel in the field be capable of or uses the present invention.
Multiple amendment to these embodiments will be apparent from for those skilled in the art, as defined herein
General Principle can realize without departing from the spirit or scope of the present invention in other embodiments.Therefore, the present invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and principles disclosed herein and features of novelty phase one
The widest scope caused.
Claims (10)
1. an extracting method for Pinus koraiensis volatile oil, including:
After Pinus koraiensis pin is mixed with distilled water, distillation extraction, after being centrifuged, draw upper strata volatile oil；The time of described distillation extraction
Being 1～3h, described Pinus koraiensis pin is 1:(2.5～4 with the liquid ratio of described distilled water).
Extracting method the most according to claim 1, it is characterised in that described Pinus koraiensis pin also wraps before mixing with distilled water
Include:
By described Pinus koraiensis pin cutting, a length of 0.8～1.2cm after the cut-out of described Pinus koraiensis pin.
Extracting method the most according to claim 1, it is characterised in that a length of 1cm after the cut-out of described Pinus koraiensis pin.
Extracting method the most according to claim 1, it is characterised in that the time of described distillation extraction is 2h.
Extracting method the most according to claim 1, it is characterised in that described liquid ratio is 1:2.5.
Extracting method the most according to claim 1, it is characterised in that described centrifugal rotating speed is 10000～12000rpm,
The described centrifugal time is 10～15min.
7. a forest air, the Pinus koraiensis volatile oil extracted including the extracting method described in any one of claim 1～6.
Forest air the most according to claim 7, it is characterised in that the content of described Pinus koraiensis volatile oil be 20vol%～
40vol%.
9. the forest air described in any one of claim 8～9 is smog air cleaner, bacteriostatic and answering in air cleaning
With.
Application the most according to claim 9, it is characterised in that described antibacterial includes escherichia coli and Staphylococcus aureus
Bacterium.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108299193A (en) * | 2018-03-27 | 2018-07-20 | 吉林派诺生物技术股份有限公司 | A kind of extraction separation method of Korean pine tower essential oil and Pinkornocid A |
| CN108837629A (en) * | 2018-04-11 | 2018-11-20 | 施勋美 | A kind of forest air and preparation system and preparation method |
| CN109498515A (en) * | 2018-12-23 | 2019-03-22 | 广州素颜皮肤管理科技有限公司 | It is a kind of have effects that repair extracts of pinus koraiensis and its application |
| CN109576061A (en) * | 2019-01-21 | 2019-04-05 | 黑龙江省林业科学研究所 | A kind of Korean Needle essential oil and its extracting method and the application in micro-organisms base |
| CN115558548A (en) * | 2022-10-28 | 2023-01-03 | 延边天翊科技有限公司 | Rosin-flavored essence for cigarettes as well as preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1552234A (en) * | 2003-05-27 | 2004-12-08 | 杭州利欣生物科技有限公司 | Feed additive for improving animal disease-resistant ability |
| CN102091337A (en) * | 2010-09-08 | 2011-06-15 | 成进学 | Nano tourmaline and fragrant flower essential oil air freshening aromatizer |
| CN104312732A (en) * | 2014-11-20 | 2015-01-28 | 邯郸学院 | Extraction method for fructus forsythia volatile oil |
| CN104399099A (en) * | 2014-10-31 | 2015-03-11 | 夏安娜 | Plant-type air freshener and preparation method thereof |
| CN105087165A (en) * | 2015-09-18 | 2015-11-25 | 哈尔滨红豆杉科技开发有限责任公司 | Composite essential oil prepared from taxus cuspidata and coniferous plants and application of composite essential oil |
| CN105219532A (en) * | 2015-09-21 | 2016-01-06 | 东北林业大学 | A kind of preparation method of Korean pine shell volatile oil |
-
2016
- 2016-05-16 CN CN201610325637.7A patent/CN106047499A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1552234A (en) * | 2003-05-27 | 2004-12-08 | 杭州利欣生物科技有限公司 | Feed additive for improving animal disease-resistant ability |
| CN102091337A (en) * | 2010-09-08 | 2011-06-15 | 成进学 | Nano tourmaline and fragrant flower essential oil air freshening aromatizer |
| CN104399099A (en) * | 2014-10-31 | 2015-03-11 | 夏安娜 | Plant-type air freshener and preparation method thereof |
| CN104312732A (en) * | 2014-11-20 | 2015-01-28 | 邯郸学院 | Extraction method for fructus forsythia volatile oil |
| CN105087165A (en) * | 2015-09-18 | 2015-11-25 | 哈尔滨红豆杉科技开发有限责任公司 | Composite essential oil prepared from taxus cuspidata and coniferous plants and application of composite essential oil |
| CN105219532A (en) * | 2015-09-21 | 2016-01-06 | 东北林业大学 | A kind of preparation method of Korean pine shell volatile oil |
Non-Patent Citations (4)
| Title |
|---|
| 朱平等: "《家庭实用理疗法手册》", 31 January 2015, 人民军医出版社 * |
| 粟本超等: "正交试验优选马尾松松针挥发油提取条件及挥发油GC-MS分析", 《安徽农业科学》 * |
| 肖阳等: "《生活保健小妙招 第2版》", 31 July 2015, 人民军医出版社 * |
| 高松等: "《辽宁中药志 植物类》", 31 December 2010, 辽宁科学技术出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108299193A (en) * | 2018-03-27 | 2018-07-20 | 吉林派诺生物技术股份有限公司 | A kind of extraction separation method of Korean pine tower essential oil and Pinkornocid A |
| CN108837629A (en) * | 2018-04-11 | 2018-11-20 | 施勋美 | A kind of forest air and preparation system and preparation method |
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| CN109576061A (en) * | 2019-01-21 | 2019-04-05 | 黑龙江省林业科学研究所 | A kind of Korean Needle essential oil and its extracting method and the application in micro-organisms base |
| CN115558548A (en) * | 2022-10-28 | 2023-01-03 | 延边天翊科技有限公司 | Rosin-flavored essence for cigarettes as well as preparation method and application thereof |
| CN115558548B (en) * | 2022-10-28 | 2023-12-08 | 延边天翊科技有限公司 | Rosin-flavored essence for cigarettes and preparation method and application thereof |
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