CN106036921A - Preparation method of soluble olive dietary fibers - Google Patents
Preparation method of soluble olive dietary fibers Download PDFInfo
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- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 98
- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 240000007817 Olea europaea Species 0.000 title abstract description 11
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- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to the technical field of food processing, and discloses a preparation method of soluble olive dietary fibers. The preparation method has the advantages of being high in efficiency, simple, convenient, low in production cost and high in extraction rate, and is favorable for favorable for popularization and application. The preparation method of the soluble olive dietary fibers disclosed by the invention comprises the following steps: step I, pretreating olives; step II, mixing products obtained after the pretreatment with a buffer system of which the pH is 4.5-5.5, and 3.5-4.5% of an enzyme, and performing a reaction at 45-55 DEG C for 1.5-2.5h; step III, performing enzyme deactivation, performing centrifugation for the first time, and performing ethanol precipitation on a supernatant; and step IV, performing centrifugation for the second time, so as to obtain precipitation which is products of the soluble olive dietary fibers.
Description
Technical field
The present invention relates to food processing technology field, particularly to the preparation method of a kind of Fructus Canarii albi water soluble dietary fiber.
Background technology
Dietary fiber is the after tradition six big nutrients (saccharide, fat, protein, water, mineral, vitamin)
Seven class nutrients, the regulation to body function is indispensable.Current numerous studies show, the modern the highest blood of multiple common disease
The colon cancer that pressure, coronary heart disease, diabetes and sickness rate are gradually increased is all closely related with the intake deficiency of dietary fiber.Can
Soluble dietary fiber can be dissolved in water, again can imbibition, and can be by the microbial degradation in large intestine, its composition includes fruit
Glue, beta glucan, polydextrose, natural gum, algin, locust beam gum, agar and hydroxymethyl cellulose etc., easily absorbed by human body intestinal canal,
There is important physiological function.
Main target currently, with respect to water soluble dietary fiber Study on Preparation is to combine biological method and chemistry, thing
Reason method, more reasonably utilizes raw material, reduces the waste discharge in production process.The existing water soluble dietary fiber prepared
Method has following several:
Physical method.Predominantly extrusion cooking method.Under high temperature, high pressure conditions, high microsteping material stability and local flavor are bright
Aobvious improving, under violent extruding condition, the water-soluble component of dietary fiber increases, but obtain is water solublity and insoluble fibre
Mixture.And this method has an obvious shortcoming when extraction has bioactive product, it is simply that to heat sensitive one-tenth
Dividing and inapplicable, the long-time extraction under high temperature is unfavorable for heat sensitive bioactive compound, causes high energy consumption, low output
Result.
Chemical method.Use organic reagent, by chemical reactions such as acid-base reactions, make dietary fiber carboxy methylation, strengthen
Its water solublity, but the method is easily caused environmental pollution.
Membrane separation process.Utilize high-tech membrane separation technique, the dietary fiber of different molecular weight is separated, it is to avoid chemistry point
From organic residue, water soluble dietary fiber has greatly prospect.
Chemical reagent and enzyme combine extraction method.After the pretreatment of chemical reagent, with α-amylase, protease, saccharifying
Other impurity of degraded such as enzyme and cellulase, then process with organic solvent, it is thus achieved that the water soluble dietary fiber that purity is higher.But enzyme
Rush method also exists deficiency with high costs, that the response time is long.
Fermentation method.With Lactobacillus bulgaricus and streptococcus thermophilus, raw material is fermented, be then washed to neutrality, be dried
After obtain dietary fiber, be mainly used in peel raw material and prepare dietary fiber.
The shortcoming existed except said method, is modified dietary fiber, improves the yield of water soluble dietary fiber also
Become research emphasis.Modification makes the connecting key of macromole rupture, and makes insoluble component be changed into soluble component, improves meals
The retention ability of food fiber and adsorption, preferably play the physiological function of dietary fiber.The functional character of dietary fiber and meals
The content of the food retention ability of fiber, swelling degree and water soluble dietary fiber has the biggest relation.Prepare dietary fiber general
Method is to soak with acid or alkali, then washes with water to neutrality, owing to washing repeatedly can reduce the retention ability of dietary fiber and swollen
Expansibility, will also result in the loss of water soluble dietary fiber simultaneously, and these processing are all unfavorable for keeping the functional character of dietary fiber,
And the content of water soluble dietary fiber is the lowest in natural dietary fiber, the most how to reduce the step of washing and improve meals
In fiber, the content of water soluble dietary fiber is the focus of research.
Fructus Canarii albi can produce a large amount of Fructus Canarii albi slag in the course of processing, and preliminary study finds, has abundant function in Fructus Canarii albi slag
Property composition, based on dietary fiber, dry weight total content be higher than 80%, if exploitation is used, can turn waste into wealth, creation can not
The economic worth estimated, brings huge social benefit.Therefore, a kind of Fructus Canarii albi solubility efficient, easy, low cost is probed into fine
Dimension preparation method has important practical significance.
Summary of the invention
In view of this, the invention discloses the preparation method of a kind of Fructus Canarii albi water soluble dietary fiber, this preparation method has
Efficiently, easy, production cost is low and extraction ratio is high advantage.
The preparation method of a kind of Fructus Canarii albi water soluble dietary fiber disclosed by the invention, comprises the following steps:
Step one, Fructus Canarii albi is carried out pretreatment；
The buffer system of step 2, the product that described pretreatment is obtained and pH 4.5～5.5 and mass concentration be 3.5～
The enzyme mixing of 4.5%, 45～55 DEG C of reactions 1.5～2.5h；
Step 3, enzyme denaturing, the most centrifugal, supernatant is carried out ethanol precipitation；
Step 4, second time are centrifugal, and gained is precipitated as product.
Preferably, described buffer system is citric acid-sodium citrate buffer.
Preferably, described enzyme is cellulase.
Preferably, the method for described enzyme denaturing is high temperature enzyme denaturing, and the temperature of described enzyme denaturing is 90 DEG C, and the time of described enzyme denaturing is
10min。
Preferably, the rotating speed that described first time is centrifuged is 4000r/min, and the time that described first time is centrifuged is 15min.
Preferably, the rotating speed that described second time is centrifuged is 4000r/min, and the time that described second time is centrifuged is 10min.
Preferably, described ethanol is precipitated as adding the dehydrated alcohol of 4 times of volumes, precipitates 1h.
Preferably, described preparation method comprises the following steps:
Step one, Fructus Canarii albi is carried out pretreatment；
Step 2, add the citric acid-sodium citrate buffer of pH 5 in ratio that solid-liquid ratio is 1:10, add 4%
Cellulase, 50 DEG C of reaction 2h；
Step 3,90 DEG C of high temperature sterilize 10min, 4000r/min is centrifuged 15min, in transfer supernatant to new container, adds
The dehydrated alcohol of 4 times of volumes, precipitates 1h；
Step 4,4000r/min are centrifuged 10min, and gained is precipitated as product.
Preprocess method in preparation method step one is to select Fructus Canarii albi fresh, that profile is intact, if there being rotten part
Excised, with 95 DEG C of hot water of the citric acid added with 0.2%, fresh olive fruits is carried out blanching treatment 5min.After burn
By fruit pelletizing, except core, dry 24h with 60 DEG C of baking oven, then put it into and pulverizer carries out pulverization process, cross 40 mesh sieves,
Make emblica powder.With sealing bag, sample is installed, and put in exsiccator and store for future use.
The present invention is to the enzyme concentration in Fructus Canarii albi soluble fiber preparation method, hydrolysis temperature, enzymolysis pH and enzymolysis time
Parameter has carried out exploitative experiment, above-mentioned preparation method parameter has also carried out the analysis of regression fit and response surface, has obtained
Optimized preparation method parameter.
In sum, the invention discloses the preparation method of a kind of Fructus Canarii albi water soluble dietary fiber.Olive disclosed by the invention
The preparation method of olive water soluble dietary fiber have efficient, easy, that elapsed time is short, production cost is low and extraction ratio is high is excellent
Point, beneficially popularization and application.Prepared Fructus Canarii albi water soluble dietary fiber has preferable retention ability and expansive force, and its retention ability is
2.64g/g, expansive force is 2.32ml/g, is that one has preferable bioactive water soluble dietary fiber product, special according to it
Property, can join in daily food or in health product as the raw material of a kind of food additive or functional food.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is only this
Inventive embodiment, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to according to
The accompanying drawing provided obtains other accompanying drawing.
Fig. 1 shows that enzyme concentration affects cartogram to water soluble dietary fiber yield；
Fig. 2 shows that hydrolysis temperature affects cartogram to water soluble dietary fiber yield；
Fig. 3 shows that enzymolysis pH affects cartogram to water soluble dietary fiber yield；
Fig. 4 shows that enzymolysis time affects cartogram to water soluble dietary fiber yield；
The three-dimensional response surface schematic diagram that Fig. 5 temp. displaying function is mutual with enzyme concentration；
The three-dimensional response surface schematic diagram that Fig. 6 temp. displaying function is mutual with pH；
Fig. 7 shows the three-dimensional response surface schematic diagram that enzyme concentration and pH are mutual.
Detailed description of the invention
The invention discloses the preparation method and applications of a kind of Fructus Canarii albi water soluble dietary fiber, this preparation method has height
Effect, the advantage that easy and production cost is low.Those skilled in the art can use for reference present disclosure, is suitably modified technological parameter real
Existing.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they
It is considered as being included in the present invention.Method and the application of the present invention are described by preferred embodiment, related personnel
Substantially in without departing from present invention, spirit and scope, method described herein and application can be modified or suitably change
With combination, realize and apply the technology of the present invention.
Below in conjunction with embodiment, the present invention is expanded on further.
Embodiment 1～3 is the embodiment preparing Fructus Canarii albi water soluble dietary fiber, and embodiment 4～8 is to Fructus Canarii albi solubility meals
The investigative test that the optimized parameter of enzyme concentration, hydrolysis temperature, enzymolysis pH and enzymolysis time in food fiber producing processes is carried out,
According to the result of the test of early stage, 6% is the more excellent yield of Fructus Canarii albi water soluble dietary fiber, and water soluble dietary fiber (SDF) is obtained
Whether rate is more than or equal to 6% as the standard selected preparation method parameter in following investigative test.
Embodiment 1
Weigh the pretreated emblica powder of 100g；The citric acid-sodium citrate of 1L pH 5.0 is added by the solid-liquid ratio of 1:10
Buffer solution, adds the cellulase of 4%, reacts 2h in the water-bath of 50 DEG C；React rear 90 DEG C of enzyme denaturing 10min,
4000r/min is centrifuged 15min, in transfer supernatant to new container, adds the dehydrated alcohol of 4 times of volumes, precipitates 1h；4000r/
Min is centrifuged 10min, and gained is precipitated as product.
Embodiment 2
Weigh the pretreated emblica powder of 100g；The citric acid-sodium citrate of 1L pH 4.5 is added by the solid-liquid ratio of 1:10
Buffer solution, adds the cellulase of 3.5%, reacts 1.5h in the water-bath of 45 DEG C；React rear 90 DEG C of enzyme denaturing 10min,
4000r/min is centrifuged 15min, in transfer supernatant to new container, adds the dehydrated alcohol of 4 times of volumes, precipitates 1h；4000r/
Min is centrifuged 10min, and gained is precipitated as product.
Embodiment 3
Weigh the pretreated emblica powder of 100g；The citric acid-sodium citrate of 1L pH 5.5 is added by the solid-liquid ratio of 1:10
Buffer solution, adds the cellulase of 4.5%, reacts 2.5h in the water-bath of 55 DEG C；React rear 90 DEG C of enzyme denaturing 10min,
4000r/min is centrifuged 15min, in transfer supernatant to new container, adds the dehydrated alcohol of 4 times of volumes, precipitates 1h；4000r/
Min is centrifuged 10min, and gained is precipitated as product.
The impact on water soluble dietary fiber yield of embodiment 4 enzyme concentration
Accurately weigh 5 parts of pretreated emblica powders of 1.0000g in test tube that is clean and that be dried, according to 1:10 (g/ml)
Solid-liquid ratio in every test tube, add the citric acid-sodium citrate buffer of 10mL pH 5.0, add to every test tube respectively
Enter the cellulase that mass concentration is 1%, 2%, 3%, 4% and 5%, the water-bath of 50 DEG C reacts 2h, has reacted rear 90
DEG C enzyme denaturing 10min, is then centrifuged 15min by sample 4000r/min, is forwarded to by supernatant in conical flask, adds the nothing of 4 times of volumes
Water-ethanol, precipitates 1h, carries out second time centrifugal, and 4000r/min is centrifuged 10min, takes precipitation and carries out water soluble dietary fiber (SDF)
The detection of yield.
Result is as shown in table 1 and Fig. 1, and when enzyme concentration is in 3～4.5%, the yield of SDF (water soluble dietary fiber) is more than
Or approximate 6%, when enzyme concentration is 4%, SDF yield is the highest, is 6.21%.When enzyme concentration is between 1～4%, SDF obtains
Rate improves along with the increase of enzyme concentration.Cellulase makes the insoluble dietary fiber in Fructus Canarii albi decompose, and makes intermolecular hydrogen
Bond fission, makes macromolecular complex be changed into little molecule, and owing to molecular mass reduces, its dissolubility also changes, a part
Insoluble dietary fiber becomes the dietary fiber of solubility, makes the yield of water soluble dietary fiber increase.And enzyme-added
Before amount reaches optimal value, concentration of substrate is bigger than enzyme concentration, added enzyme can not enzymolysis insoluble dietary fiber substrate completely,
But along with the increase enzyme concentration of enzyme concentration is consequently increased, energy enzymolysis more insoluble dietary fiber substrate, therefore along with fibre
The increase of dimension element enzyme enzyme concentration, the yield of water soluble dietary fiber is consequently increased.But it is after enzyme concentration is more than 4%, unnecessary
Cellulase can become enzymolysis the little molecule of the water soluble dietary fiber further enzymolysis Cheng Geng little of little molecule, as many
Sugar, oligosaccharide and monosaccharide, when precipitating with ethanol, this kind of material can not be precipitated out by ethanol, therefore causes in Fructus Canarii albi
The yield of water soluble dietary fiber declines along with the increase of enzyme concentration.Consider, 3～4.5% enzyme concentration for preparing olive
The preferred enzyme concentration of olive water soluble dietary fiber, 4% is optimal enzyme concentration.
The impact on SDF yield of table 1 enzyme concentration
The impact on water soluble dietary fiber yield of embodiment 5 hydrolysis temperature
Accurately weigh 5 parts of pretreated emblica powders of 1.0000g in test tube that is clean and that be dried, according to 1:10 (g/ml)
Solid-liquid ratio in every test tube, add the citric acid-sodium citrate buffer of 10mL pH 5.0, add in every test tube
Mass concentration is the cellulase of 4%, reacts 2h, reaction respectively in the water-bath of 30 DEG C, 40 DEG C, 50 DEG C, 60 DEG C and 70 DEG C
Complete rear 90 DEG C of enzyme denaturing 10min, are then centrifuged 15min by sample 4000r/min, are forwarded to by supernatant in conical flask, add 4 times of bodies
Long-pending dehydrated alcohol, precipitates 1h, carries out second time centrifugal, and 4000r/min is centrifuged 10min, takes precipitation and carries out the inspection of SDF yield
Survey.
Result is as shown in table 2 and Fig. 2, and when reaction temperature is 45～55 DEG C, SDF yield is greater than or approximately equal to 6%, reaction
When temperature is 50 DEG C, SDF yield is the highest, and 6.26%.When hydrolysis temperature is at 30～50 DEG C, Fructus Canarii albi water soluble dietary fiber
Rate increases along with the rising of temperature, and when 50 DEG C, yield is the highest, is because when hydrolysis temperature is 30～50 DEG C, cellulase
Activity increase along with the rising of temperature, 50 DEG C is the optimal reactive temperature of cellulase.When temperature is more than 50 DEG C, cellulose
The activity of enzyme is affected by the high temperature and declines, and causes enzymolysis efficiency to reduce, therefore when temperature obtaining more than 50 DEG C of soluble fiber
Rate declines along with the rising of temperature.Consider, 45～55 DEG C of preferred enzymolysis temperature being to prepare Fructus Canarii albi water soluble dietary fiber
Degree, 50 DEG C is optimal hydrolysis temperature.
The impact on SDF yield of table 2 hydrolysis temperature
The embodiment 6 enzymolysis pH impact on water soluble dietary fiber yield
Accurately weigh 5 parts of pretreated emblica powders of 1.0000g in test tube that is clean and that be dried, according to 1:10 (g/ml)
Solid-liquid ratio respectively in every test tube add pH 3.0, pH 4.0, the citric acid-sodium citrate buffer of pH 5.0, and
PH 6.0, the phosphate buffer of pH 7.0, adding mass concentration in every test tube is the cellulase of 4%, at the water of 50 DEG C
Bath reacts 2h, has reacted rear 90 DEG C of enzyme denaturing 10min, then sample 4000r/min has been centrifuged 15min, supernatant is forwarded to
In conical flask, adding the dehydrated alcohol of 4 times of volumes, precipitate 1h, carry out second time centrifugal, 4000r/min is centrifuged 10min, and it is heavy to take
Form sediment and carry out the detection of SDF yield.
Result is as shown in table 3 and Fig. 3, and when pH is 4.5～5.5, SDF yield is greater than or approximately equal to 6%, the SDF when pH is 5
Yield is the highest, is 6.41%.When enzymolysis pH is between 3.0～5.0, the yield of Fructus Canarii albi water soluble dietary fiber is along with enzymolysis pH
Rising and increase, but after enzymolysis pH is more than 5.0, soluble fiber yield but declines along with the rising of pH, this is because
The activity of enzyme is affected by its environmental pH, and pH value is too high or too low all can produce impact to the efficiency of enzyme digestion reaction.Comprehensively
Considering, pH 4.5～5.5 is the preferred enzymolysis pH preparing Fructus Canarii albi water soluble dietary fiber, and pH 5 is optimal enzymolysis pH.
Table 3 pH impact on SDF yield
The impact on water soluble dietary fiber yield of embodiment 7 enzymolysis time
Accurately weigh 5 parts of pretreated emblica powders of 1.0000g in test tube that is clean and that be dried, according to 1:10 (g/ml)
Solid-liquid ratio in every test tube, add the citric acid-sodium citrate buffer of pH 5.0 respectively, add matter to every test tube
Amount concentration is the cellulase of 4%, reacts 1h, 2h, 3h, 4h and 5h respectively, at 90 DEG C after having reacted in the water-bath of 50 DEG C
Lower enzyme denaturing 10min, is then centrifuged 15min by sample 4000r/min, is forwarded to by supernatant in conical flask, adds the nothing of 4 times of volumes
Water-ethanol, precipitates 1h, carries out second time centrifugal, and 4000r/min is centrifuged 10min, takes precipitation and carries out the detection of SDF yield.
Result is as shown in table 4 and Fig. 4, and when enzymolysis time is 1.5～2.5h, SDF yield is greater than or approximately equal to 6%, during enzymolysis
Between when being 2h the yield of water soluble dietary fiber the highest, be 6.4%.When enzymolysis time is in 1～2h, water soluble dietary fiber
Yield increases along with the increase in response time, is because when enzymolysis time is in 1～2h, enzyme-to-substrate haptoreaction, fiber
Insoluble dietary fiber enzymolysis is water soluble dietary fiber by element enzyme, when enzymolysis time is more than 2h, water soluble dietary fiber quilt
Enzymolysis becomes the little molecules such as polysaccharide, oligosaccharide and monosaccharide further, therefore when enzymolysis time obtaining more than 2h water soluble dietary fiber
Rate reduces along with the increase of enzymolysis time.Considering, 1.5～2.5h is the preferred enzyme preparing Fructus Canarii albi water soluble dietary fiber
The solution time, 2h is optimal enzymolysis time.
The impact on SDF yield of table 4 enzymolysis time
Embodiment 8 response surface design and optimization
On the basis of experiment of single factor, according to Box-Behnken EXPERIMENTAL DESIGN principle, experimental program is designed, choosing
Taken hydrolysis temperature (A), enzyme concentration (B), enzymolysis pH (C) are independent variable, using the extraction ratio of water soluble dietary fiber as response
Value, use Three factors-levels Response surface methodology carry out EXPERIMENTAL DESIGN, each factor be horizontally disposed with and encoded radio is shown in Table
5。
Table 5 response surface analysis factor and water-glass
Utilize Design Expert V8.0.6 software that the test data in table 1 is carried out linear return two times matching,
To mathematical model, i.e. the sign equation of Fructus Canarii albi water soluble dietary fiber yield (extraction ratio):
Y=6.55-0.26A-0.024B+0.75C-0.26AB+0.31AC-0.33BC-1.5A2-1.1 4B2-1.45C2
This model coefficient significance test is shown in Table 6, and model the results of analysis of variance is shown in Table 7.
Table 6 regression equation coefficient significance test
Note: " P < 0.01 " is the extremely notable * * of difference；" P < 0.05 " is significant difference *.
Table 7 regression model variance analysis
From table 3 it can be seen that F value is 43.19 (P < 0.01), illustrates that the regression model of gained is extremely notable, and it is poor to lose plan item
Different not notable (P=0.1441 > 0.05).The determination coefficients R of model2=0.9823, model adjusts and determines coefficients R Adj2=
0.9596, illustrate that this model can explain the change of 95.96% response value, therefore the fitting degree of model is preferable, can be used for prediction originally
The response value of test gained quadratic regression equation, i.e. the extraction ratio of water soluble dietary fiber in Fructus Canarii albi.
Knowable to table 2 regression equation coefficient significance test, in water soluble dietary fiber extraction ratio model first order (C) and
Quadratic term (A2, B2, C2) is extremely notable on the impact of SDF extraction ratio, and first order (A) impact is notable, first order (B) and mutual item (AB,
AC, BC) impact the most notable.Therefore, the extraction ratio of Fructus Canarii albi water soluble dietary fiber is affected the most prominent by enzymolysis pH, it may be possible to because of
The pH environment bigger on the impact of enzyme activity, different for pH, plays its zymolysis and produces different impacts, optimum pH enzyme
PH value in addition, all produces certain inhibitory action to enzyme activity, even makes enzyme inactivate.The impact of temperature also has significantly, its
Main cause is also likely to be the temperature effect to enzyme, and beyond the optimum temperature of enzyme, temperature is below or above optimum temperature, all can
Inhibitory enzyme vigor, makes enzymolysis efficiency reduce, and temperature is too high, enzyme even can be made to inactivate, thus affect its enzymolysis efficiency.
Fig. 5 to Fig. 7 be Fructus Canarii albi water soluble dietary fiber extraction ratio characterize the response surface design that done of equation and equal pitch contour thereof
Figure.Thus the extraction ratio of any two factor interaction influence Fructus Canarii albi water soluble dietary fiber can be analyzed and evaluates, with
Determine with optimal factor level scope that the extraction ratio of Fructus Canarii albi water soluble dietary fiber is response target.
Fig. 5 shows that factor A-temperature and factor B-enzyme concentration are solvable to Fructus Canarii albi when factor C-enzymolysis pH value is equal to 5.0
The reciprocal effect of property dietary fiber extraction ratio.From fig. 5, it can be seen that in the range of factor is investigated, when pH value is constant, along with
Hydrolysis temperature and the increase of enzyme concentration, after the extraction of water soluble dietary fiber takes the lead in being gradually increased to certain value, again in decline
Trend.Extraction ratio gradually rises the suitable condition that has been because, and enzyme activity increases, and accelerates the speed of enzymolysis, makes insoluble
Dietary fiber becomes water soluble dietary fiber, improves its extraction ratio, but when temperature is too high, enzyme activity declines, so
Decompose the decline of the ability of dietary fiber, and along with the increase of enzyme concentration, the concentration of enzyme is excessive, is divided by water soluble dietary fiber
Solution becomes less molecule, it is impossible to be precipitated out by dehydrated alcohol.Therefore, in the case of pH value is constant, hydrolysis temperature and enzyme-added
There is extreme value in the response surface design of amount, at hydrolysis temperature 45～55 DEG C and enzyme concentration in the range of 3.5～4.5%, and soluble dietary
The extraction ratio of fiber is bigger.
Fig. 6 shows that factor A-temperature and factor C-enzymolysis pH value are solvable to Fructus Canarii albi when factor B-enzyme concentration is equal to 4%
The reciprocal effect of property dietary fiber extraction ratio.From fig. 6, it can be seen that in the range of factor is investigated, when enzyme concentration is constant, with
The increase of hydrolysis temperature and pH, after the extraction of water soluble dietary fiber takes the lead in being gradually increased to certain value, again in becoming of declining
Gesture.Therefore, in the case of enzyme concentration is constant, there is extreme value in the response surface design of hydrolysis temperature and enzyme concentration, at hydrolysis temperature 45
～in the range of 55 DEG C and pH 4.5～5.5, the extraction ratio of water soluble dietary fiber is bigger.
Fig. 7 shows that factor B-enzyme concentration and factor C-enzymolysis pH value are to olive when factor A-hydrolysis temperature is equal to 50 DEG C
The reciprocal effect of olive water soluble dietary fiber extraction ratio.From figure 7 it can be seen that in the range of factor is investigated, temperature-resistant
Time, along with enzyme concentration and the increase of pH, after the extraction of water soluble dietary fiber takes the lead in being gradually increased to certain value, again in decline
Trend.Therefore, in the case of temperature-resistant, there is extreme value in the response surface design of enzyme concentration and pH, at enzyme concentration 3.5～4.5%
In the range of pH 4.5～5.5, the extraction ratio of water soluble dietary fiber is bigger.
Embodiment 9 retention ability and the mensuration of expansive force
The water soluble dietary fiber accurately weighing 0.5000g embodiment 1 preparation is placed in 10mL centrifuge tube, adds 7mL and steams
Distilled water, at room temperature soaks 24h after being sufficiently stirred for, then centrifugal 10min under conditions of 3500r/min, removes supernatant, and
Excessive moisture filter paper in inwall is blotted, weighs the quality of precipitation, be calculated as follows retention ability, in triplicate.Result
As shown in table 8, the retention ability recorded is 2.64g/g.The water soluble dietary fiber prepared by embodiment 2 and embodiment 3 repeats same
The test of sample, obtains same result.
The dietary fiber powder accurately weighing 0.5000g embodiment 1 preparation is placed in 10mL scale test tube, records solubility
The volume of dietary fiber, at room temperature adds 5mL distilled water, shaken well, at room temperature places 24h, observes sample at test tube
In free wxpansion volume (mL), represent its expansive force, in triplicate with the expansion volume (mL/g) of every gram of dry.Result
As shown in table 9, the expansive force recorded is 2.32ml/g.The water soluble dietary fiber prepared by embodiment 2 and embodiment 3 repeats same
The test of sample, obtains same result.
Table 8 SDF retention ability measurement result
Table 9 SDF expansive force measurement result
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For Yuan, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (8)
1. the preparation method of a Fructus Canarii albi water soluble dietary fiber, it is characterised in that comprise the following steps:
Step one, Fructus Canarii albi is carried out pretreatment；
The buffer system of step 2, the product that described pretreatment is obtained and pH 4.5～5.5 and mass concentration be 3.5～
The enzyme mixing of 4.5%, 45～55 DEG C of reactions 1.5～2.5h；
Step 3, enzyme denaturing, the most centrifugal, supernatant is carried out ethanol precipitation；
Step 4, second time are centrifugal, and gained is precipitated as product.
Preparation method the most according to claim 1, it is characterised in that described buffer system is that citric acid-sodium citrate is delayed
Rush liquid.
Preparation method the most according to claim 1, it is characterised in that described enzyme is cellulase.
Preparation method the most according to claim 1, it is characterised in that the method for described enzyme denaturing is high temperature enzyme denaturing, described in go out
The temperature of enzyme is 90 DEG C, and the time of described enzyme denaturing is 10min.
Preparation method the most according to claim 1, it is characterised in that the rotating speed that described first time is centrifuged is 4000r/min,
The time that described first time is centrifuged is 15min.
Preparation method the most according to claim 1, it is characterised in that the rotating speed that described second time is centrifuged is 4000r/min,
The time that described second time is centrifuged is 10min.
Preparation method the most according to claim 1, it is characterised in that described ethanol is precipitated as adding described supernatant 4 times
The dehydrated alcohol of volume, precipitates 1h.
Preparation method the most according to claim 1, it is characterised in that comprise the following steps:
Step one, Fructus Canarii albi is carried out pretreatment；
Step 2, adding the citric acid-sodium citrate buffer of pH 5 in ratio that solid-liquid ratio is 1:10, adding mass concentration is
The cellulase of 4%, 50 DEG C of reaction 2h；
Step 3,90 DEG C of high temperature enzyme denaturing 10min, 4000r/min is centrifuged 15min, shifts supernatant, adds the anhydrous of 4 times of volumes
Ethanol, precipitates 1h；
Step 4,4000r/min are centrifuged 10min, and gained is precipitated as product.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106880057A (en) * | 2017-04-26 | 2017-06-23 | 广东工业大学 | The preparation method and leaf mustard diet fiber product of a kind of leaf mustard dietary fiber |
| CN107319084A (en) * | 2017-07-31 | 2017-11-07 | 福州大世界橄榄有限公司 | A kind of modified olive dietary fiber soft sweets and preparation method thereof |
| CN113841721A (en) * | 2021-09-29 | 2021-12-28 | 陇南市祥宇油橄榄开发有限责任公司 | Olive dietary fiber biscuit and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101283760A (en) * | 2008-05-27 | 2008-10-15 | 中国食品发酵工业研究院 | A method for extracting and preparing meal fibre from the peach dregs |
| CN101695364A (en) * | 2009-10-15 | 2010-04-21 | 新疆农业大学 | Method for preparing dietary fiber by apricot residue |
-
2016
- 2016-05-25 CN CN201610357526.4A patent/CN106036921A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101283760A (en) * | 2008-05-27 | 2008-10-15 | 中国食品发酵工业研究院 | A method for extracting and preparing meal fibre from the peach dregs |
| CN101695364A (en) * | 2009-10-15 | 2010-04-21 | 新疆农业大学 | Method for preparing dietary fiber by apricot residue |
Non-Patent Citations (3)
| Title |
|---|
| 乐胜锋，等: "纤维素酶法提取苹果渣可溶性膳食纤维", 《食品研究与开发》 * |
| 欧高政，等: "橄榄果实膳食纤维含量及动态变化研究", 《福建农业学报》 * |
| 牟建楼，等: "不同提取方法对苹果渣中可溶性膳食纤维的影响", 《中国食品学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106880057A (en) * | 2017-04-26 | 2017-06-23 | 广东工业大学 | The preparation method and leaf mustard diet fiber product of a kind of leaf mustard dietary fiber |
| CN107319084A (en) * | 2017-07-31 | 2017-11-07 | 福州大世界橄榄有限公司 | A kind of modified olive dietary fiber soft sweets and preparation method thereof |
| CN113841721A (en) * | 2021-09-29 | 2021-12-28 | 陇南市祥宇油橄榄开发有限责任公司 | Olive dietary fiber biscuit and preparation method thereof |
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