CN106032544A - Method for improving enzymatic hydrolysis rate of lignocellulose biomass with liquid ammonia low temperature pre-dipping and synergistic pretreatment by Funalia trogii - Google Patents
Method for improving enzymatic hydrolysis rate of lignocellulose biomass with liquid ammonia low temperature pre-dipping and synergistic pretreatment by Funalia trogii Download PDFInfo
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- CN106032544A CN106032544A CN201510120457.0A CN201510120457A CN106032544A CN 106032544 A CN106032544 A CN 106032544A CN 201510120457 A CN201510120457 A CN 201510120457A CN 106032544 A CN106032544 A CN 106032544A
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Abstract
The invention discloses a method for improving enzymatic hydrolysis rate of lignocellulose biomass with liquid ammonia low temperature pre-dipping and synergistic pretreatment by Funalia trogii. The lignocellulose biomass can be converted in order to prepare cellulosic ethanol which has important economic values, establishment of appropriate pretreatment technology for changing physical and chemical structures of the lignocellulose biomass and improving the enzymatic hydrolysis rate is an important step. The invention establishes technology for pretreatment of lignocellulose biomass with liquid ammonia low temperature pre-dipping and synergistic Funalia trogii. The method comprises the following steps: the lignocellulose biomass is crushed in order to carry out liquid ammonia pre-dipping; after pre-dipping with liquid ammonia, the lignocellulose biomass is dried and inoculated with seed liquid of Funalia trogii, in order to carry out pretreatment with Funalia trogii; after pretreatment with Funalia trogii, the lignocellulose biomass is washed in order to remove water soluble substances on the surface, filtering is carried out, and drying and enzymatic hydrolysis are carried out. Lignin can be substantially removed, few inhibitors for enzymatic hydrolysis and fermentation are generated in the reaction process, corrosivity is not generated, the action process is relatively mild, and recycle is easy. The new method for utilization of lignocellulose biomass with high quality is provided.
Description
Technical field
The invention belongs to biomass energy source domain, work in coordination with, particularly to a kind of liquefied ammonia low temperature prepregs, the method that bristle thick cap bore bacterium pretreatment improves lignocellulose biomass enzymatic hydrolyzation.
Background technology
The energy is the basis that human society is depended on for existence and development.Traditional fossil energy non-renewable, and utilize the environmental pollution and greenhouse effect caused so that it is the demand of human economic society development can not be met.Bio-ethanol is high with its octane number, safety is good, can substitute methyl tertiary butyl ether(MTBE), ethyl tert-butyl oxide as oxygenated additive, it is possible to replacing gasoline completely receives much concern as fuel.China's lignocellulose biomass aboundresources, lignocellulose biomass rich cellulose, is the desirable feedstock of exploitation cellulosic ethanol.Preparing cellulosic ethanol with lignocellulose biomass for development of raw materials, to contribute to realizing biomass high-valued, improves farmers' income, increases Business Economic Benefit, promotes China's economic development and energy industry structure updating, have important Economic Application prospect.
Pretreatment is the essential step of cellulosic ethanol production, and pretreatment cost often accounts for whole production cost 1/3rd.Pretreatment purpose is to change biomass macroscopic view, micro-phase action, and chemical composition, to promote cellulase hydrolysis.Pretreatment comprises physics pretreatment and Chemical Pretreatment, and physical chemistry pretreatment.Liquefied ammonia preimpregnation has long research applicating history, was once widely used in straw ammoniation modification and prepares animal feed.Utilize liquefied ammonia preimpregnation pretreatment to change biomass physical arrangement, separating lignin, destroy hemicellulose and lignin in biomass and be interweaved, interact, modified biomass structure, and then promote biomass enzymatic saccharification.But it is longer that liquefied ammonia preimpregnation typically requires at high temperature effect or action time, and this limits its range of application to a certain extent.The present invention have developed a kind of liquefied ammonia low temperature prepregs and works in coordination with the technique of bristle thick cap bore bacterium pretreatment, improves biomass enzymatic saccharification rate by two kinds of pretreatment synergism, lays the first stone for the high-valued application of biomass.
Summary of the invention
It is an object of the present invention to provide a kind of liquefied ammonia low temperature prepregs and work in coordination with the method that bristle thick cap bore bacterium pretreatment improves lignocellulose biomass enzymatic hydrolyzation, i.e. utilize finite concentration liquefied ammonia aqueous solution to presoak lignocellulose biomass, destroy lignocellulose biomass physicalchemical structure.The lignocellulose biomass of liquefied ammonia preimpregnation carries out bristle thick cap bore bacterium pretreatment again.By the two collaborative pretreatment to improve enzymatic hydrolyzation, shorten enzymolysis time and use enzyme amount.
A kind of liquefied ammonia low temperature prepregs works in coordination with the method that bristle thick cap bore bacterium pretreatment improves lignocellulose biomass enzymatic hydrolyzation
Technical scheme is as follows:
(1) 20 ~ 40 mesh sieves are crossed after lignocellulose biomass is pulverized；
(2) putting in pressure vessel after the lignocellulose biomass pulverized and liquefied ammonia aqueous solution, reaction collects filtering residue after terminating, and obtains the lignocellulose biomass of liquefied ammonia preimpregnation；
(3) lignocellulose biomass of liquefied ammonia preimpregnation accesses bristle thick cap bore bacterium seed liquor after drying, and carries out bristle thick cap bore bacterium pretreatment；
(4) the lignocellulose biomass washing of bristle thick cap bore bacterium pretreatment removes the surface hydrotrope, filters, enzymolysis after drying.
The lignocellulose biomass of step (1) includes the one in Caulis et Folium Oryzae, wheat straw, corn straw, bagasse and megasse.
The liquid ammonia solution mass concentration of step (2) is 5 g/L ~ 25 g/L, and lignocellulose biomass quality and liquid ammonia solution mass ratio are 1:6 ~ 1:20, reaction temperature 40 ~ 100 DEG C, response time 1 ~ 20h.
The seed liquor of step (3) is bristle thick cap bore bacterium fermentation liquid of 4 ~ 5 days gained of liquid fermentation in potato culture；Seed liquor inoculum concentration is the 6% ~ 11% of lignocellulose biomass quality.
The bristle thick cap bore bacterium pretreatment condition of step (3) is water content 60% ~ 83%, temperature 22 ~ 35 DEG C, 3 ~ 10 days time.
The washing conditions substrate quality of step (4) and the mass ratio of water are 1:5 ~ 1:15, soak 15 ~ 60 min.
A kind of liquefied ammonia low temperature prepregs works in coordination with the method that bristle thick cap bore bacterium pretreatment improves lignocellulose biomass enzymatic hydrolyzation, and it has the advantage that
(1) liquid ammonia solution is alkalescence, destroys the C-O-C link between fracture lignin and lignin carbohydrate complexes, remove lignin in preimpregnation course of reaction.
(2) pre-treatment of matrix nitrogen content is improved.
(3) less enzymolysis, fermentation inhibitor are produced.
(4) mechanism is relatively mild, it is easy to recycle.
Detailed description of the invention
Embodiment 1
Taking the rice straw powder 20g of 20 mesh sieves, and added the preimpregnation of liquefied ammonia aqueous solution, liquid ammonia solution mass concentration is 15g/L, and Caulis et Folium Oryzae quality and liquid ammonia solution mass ratio are 1:6, reaction temperature 80 DEG C, response time 6h.The Caulis et Folium Oryzae of liquefied ammonia preimpregnation accesses the bristle thick cap bore bacterium seed liquor of 6% after drying, regulation water content of substrate is 75%, and 28 DEG C carry out bristle thick cap bore bacterium pretreatment 5 days.The Caulis et Folium Oryzae of pretreatment is 1:5 with the quality solid-to-liquid ratio of water, soaks 30min, and the surface hydrotrope is removed in eccysis, filters, and obtains rice straw, enzymatic saccharification 72h, measure glucose content in enzymolysis solution after drying, and straw cellulose enzymatic hydrolyzation is 72%.
Embodiment 2
Taking the corn stalk powder 20g of 20 mesh sieves, and added the preimpregnation of liquefied ammonia aqueous solution, liquid ammonia solution mass concentration is 20g/L, and Caulis et Folium Oryzae quality and liquid ammonia solution mass ratio are 1:8, reaction temperature 90 DEG C, response time 8h.The corn stalk powder of liquefied ammonia preimpregnation accesses the bristle thick cap bore bacterium seed liquor of 6% after drying, regulation water content of substrate is 75%, and 28 DEG C carry out bristle thick cap bore bacterium pretreatment 5 days.The Caulis et Folium Oryzae of pretreatment is 1:5 with the quality solid-to-liquid ratio of water, soaks 30min, and the surface hydrotrope is removed in eccysis, filters, and obtains corn straw substrate, enzymatic saccharification 72h, measure glucose content in enzymolysis solution after drying, and corn stalk fiber element enzymatic hydrolyzation is 81%.
Embodiment 3
Taking the barley seeding food 20g of 20 mesh sieves, and added the preimpregnation of liquefied ammonia aqueous solution, liquid ammonia solution mass concentration is 10g/L, and wheat straw quality and liquid ammonia solution mass ratio are 1:6, reaction temperature 60 DEG C, response time 4h.The barley seeding food of liquefied ammonia preimpregnation accesses the bristle thick cap bore bacterium seed liquor of 6% after drying, regulation water content of substrate is 70%, and 28 DEG C carry out bristle thick cap bore bacterium pretreatment 3 days.The barley seeding food of pretreatment is 1:10 with the quality solid-to-liquid ratio of water, soaks 15min, and the surface hydrotrope is removed in eccysis, filters, and obtains wheat straw substrate, enzymatic saccharification 72h, measure glucose content in enzymolysis solution after drying, and wheatgrass cellulose enzymatic hydrolyzation is 61%.
Embodiment 4
Taking the bagasse powder 20g of 30 mesh sieves, and added the preimpregnation of liquefied ammonia aqueous solution, liquid ammonia solution mass concentration is 25g/L, and bagasse quality and liquid ammonia solution mass ratio are 1:10, reaction temperature 70 DEG C, response time 8h.The barley seeding food of liquefied ammonia preimpregnation accesses the bristle thick cap bore bacterium seed liquor of 8% after drying, regulation water content of substrate is 73%, and 30 DEG C carry out bristle thick cap bore bacterium pretreatment 6 days.The barley seeding food of pretreatment is 1:15 with the quality solid-to-liquid ratio of water, soaks 25min, and the surface hydrotrope is removed in eccysis, filters, and obtains Caulis Sacchari sinensis substrate, enzymatic saccharification 72h, measure glucose content in enzymolysis solution after drying, and Caulis Sacchari sinensis fiber element enzymatic hydrolyzation is 75%.
Embodiment 5
Taking the Radix Betae ground-slag 20g of 40 mesh sieves, and added the preimpregnation of liquefied ammonia aqueous solution, liquid ammonia solution mass concentration is 25g/L, and bagasse quality and liquid ammonia solution mass ratio are 1:10, reaction temperature 85 DEG C, response time 8h.The barley seeding food of liquefied ammonia preimpregnation accesses the bristle thick cap bore bacterium seed liquor of 6% after drying, regulation water content of substrate is 80%, and 32 DEG C carry out bristle thick cap bore bacterium pretreatment 9 days.The megasse of pretreatment is 1:15 with the quality solid-to-liquid ratio of water, soaks 45min, and the surface hydrotrope is removed in eccysis, filters, and obtains megasse substrate, enzymatic saccharification 72h, measure glucose content in enzymolysis solution after drying, and megasse cellulase hydrolysis rate is 45%.
Claims (6)
1. a liquefied ammonia low temperature prepregs works in coordination with the method that bristle thick cap bore bacterium pretreatment improves lignocellulose biomass enzymatic hydrolyzation, it is characterised in that following steps:
(1) 20 ~ 40 mesh sieves are crossed after lignocellulose biomass is pulverized；
(2) putting in pressure vessel after the lignocellulose biomass pulverized and liquefied ammonia aqueous solution, reaction collects filtering residue after terminating, and obtains the lignocellulose biomass of liquefied ammonia preimpregnation；
(3) lignocellulose biomass of liquefied ammonia preimpregnation accesses bristle thick cap bore bacterium seed liquor after drying, and carries out bristle thick cap bore bacterium pretreatment；
(4) the lignocellulose biomass washing of bristle thick cap bore bacterium pretreatment removes the surface hydrotrope, filters, enzymolysis after drying.
Method the most according to claim 1, is characterised by that the lignocellulose biomass of described step (1) includes the one in Caulis et Folium Oryzae, wheat straw, corn straw, bagasse and megasse.
Method the most according to claim 1, is characterised by that the liquid ammonia solution mass concentration of described step (2) is 5 g/L ~ 25 g/L, and lignocellulose biomass quality and liquid ammonia solution mass ratio are 1:6 ~ 1:20, reaction temperature 40 ~ 100 DEG C, response time 1 ~ 20h.
Method the most according to claim 1, is characterised by that the seed liquor of described step (3) is bristle thick cap bore bacterium fermentation liquid of 4 ~ 5 days gained of liquid fermentation in potato culture；Seed liquor inoculum concentration is the 6% ~ 11% of lignocellulose biomass quality.
Method the most according to claim 1, is characterised by that the bristle thick cap bore bacterium pretreatment condition of described step (3) is: water content 60% ~ 83%, temperature 22 ~ 35 DEG C, 3 ~ 10 days time.
Method the most according to claim 1, is characterised by the washing conditions 5 of described step (4)～15 times of water soaking 15 ~ 60 min.
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| CN201510120457.0A CN106032544A (en) | 2015-03-19 | 2015-03-19 | Method for improving enzymatic hydrolysis rate of lignocellulose biomass with liquid ammonia low temperature pre-dipping and synergistic pretreatment by Funalia trogii |
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| CN201510120457.0A CN106032544A (en) | 2015-03-19 | 2015-03-19 | Method for improving enzymatic hydrolysis rate of lignocellulose biomass with liquid ammonia low temperature pre-dipping and synergistic pretreatment by Funalia trogii |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109337937A (en) * | 2018-11-16 | 2019-02-15 | 安徽工业大学 | A method of utilizing tetrahydrofurfuryl alcohol preprocessing lignocellulose |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109337937A (en) * | 2018-11-16 | 2019-02-15 | 安徽工业大学 | A method of utilizing tetrahydrofurfuryl alcohol preprocessing lignocellulose |
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