CN106018050A - Frozen section embedding agent - Google Patents
Frozen section embedding agent Download PDFInfo
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- CN106018050A CN106018050A CN201610342036.7A CN201610342036A CN106018050A CN 106018050 A CN106018050 A CN 106018050A CN 201610342036 A CN201610342036 A CN 201610342036A CN 106018050 A CN106018050 A CN 106018050A
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- frozen section
- polyvinyl alcohol
- embedding medium
- purified water
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Medicinal Preparation (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a frozen section embedding agent which is prepared from the following components in parts by mass: 100 parts of purified water, 80-100 parts of polyvinyl alcohol 124, 30-50 parts of polyvinylpyrrolidone, 10-20 parts of sodium carboxymethylcellulose and 50-80 parts of polyethylene glycol 300. The embedding agent designed in the invention has good heat conduction, transparency, viscosity, water solubility and extension, and is proper in hardness. The frozen section embedding agent disclosed by the invention can be used for embedding tissues during pathological diagnosis in an operation, and also can be used for normal pathological diagnosis, an immunologic tissue chemical diagnosis technology and a molecular biological diagnosis technology.
Description
Technical field
The present invention relates to external diagnosis reagent, particularly to a kind of frozen section embedding medium.
Background technology
Routine pathology diagnosis is exactly that surgeon's tissue of taking off of operation delivers to Pathology Deparment, and pathology technique doctor is by right
Tissue carries out specimen and prepares (fixing, dehydration, transparent, waxdip)；Embed, cut into slices, bake sheet；Dyeing (dewaxing, Lignum Sappan
Element helps dye, haematoxylin dyeing, differentiation, oil blackeite, eosin stains, transparent, mounting) after, give disease the section dyeed
Reason diagnostician, the disease of patient to sections observation, is made pathological diagnosis by microscope by diagnostician.This process one
As take about 3 days, such pathology routine diagnosis to surgery surgical treatment just seem delayed, be likely to result in patient's secondary
Operative treatment, causes unnecessary misery to patient.For making pathological diagnosis Tong Bu with surgical operation therapy, pathology operation is examined
Break and just create.In pathology operation, diagnosis is exactly that the diseased tissue taken off is delivered to rapidly Pathology Deparment, pathology skill by surgeon
Art doctor is immediately by carrying out freezing to diseased tissue, by microtome, dyeing, mounting.Pathological diagnosis doctor pass through
Microscope carries out inspections and examinations to section, then gives surgeon by diagnostic result, and operative doctor is according to pathologist
Diagnostic result, is disposed patient.Pathology operation diagnoses whole process and typically completed at about 30 minutes, during this
Patient is on operating-table, and operation stays cool.In pathology operation, the meaning of diagnosis is that pathological diagnosis is same with surgical operation
Step is carried out, if pathological diagnosis is optimum, then surgical operation terminates, otherwise pathological diagnosis is pernicious, then surgery
Operation proceeds operative treatment according to pernicious treatment means.Avoid second operation, strive for treatment time.Frost is cut
Chip technology requires first to embed tissue with embedding medium, by embedded tissue after suitable temperature freezing, right
Tissue is cut into slices.Acting as of embedding medium: to organizing supporting role, reduces blade and organizes thin to tissue pressure, holding
Born of the same parents' original shape state；There is the water absorption of appropriateness, it is ensured that tissue does not produce ice crystal, therefore it is required that embedding medium has characteristics that time freezing
Heat conductivity, transparency, toughness, suitable stiffness, ductility, solubization.The all imports of embedding medium of Hospitals at Present
Product, indices quality differs, and expensive, and because the useful paste of price problem replaces embedding medium, this is not to solve
The certainly way of problem, its effect is extremely difficult to technology requirement.
Summary of the invention
It is an object of the invention to solve at least the above, and the advantage that at least will be described later is provided.
It is a still further object of the present invention to provide a kind of frozen section embedding medium, heat conductivity that its tool is good, transparency, thickness
Property and ductility.
In order to realize according to object of the present invention and further advantage, it is provided that a kind of frozen section embedding medium, including following
The component of mass fraction:
Purified water 1000 parts, polyvinyl alcohol 124 80～100 parts, PVPK30～50 parts, carboxymethyl cellulose
Sodium 10～20 parts and Liquid Macrogol 50～80 parts；
Purified water, polyvinyl alcohol 124, polyvinylpyrrolidone, sodium carboxymethyl cellulose and Liquid Macrogol are mixed,
It is heated to 95 DEG C, is stirring, i.e. obtain frozen section embedding medium.
Preferably, in described frozen section embedding medium, including the component of following mass fraction:
Purified water 1000 parts, polyvinyl alcohol 124 90 parts, polyvinylpyrrolidone 35 parts, sodium carboxymethyl cellulose 15 parts
With Liquid Macrogol 60 parts.
Preferably, in described frozen section embedding medium, polyvinyl alcohol 124, polyvinylpyrrolidone, carboxymethyl cellulose
Element sodium and Liquid Macrogol are analytical pure.
Preferably, in described frozen section embedding medium, including the component of following mass fraction:
Purified water 1000 parts, polyvinyl alcohol 124 90 parts, PVP part, sodium carboxymethyl cellulose 15 parts,
Liquid Macrogol 70 parts, collagen protein 2 parts, thymol 1 part, agar 1 part, carrageenan 7 parts, sodium alginate
11 parts, spermol 0.3 part and ethyl hydroxybenzoate 0.1 part.
Preferably, in described frozen section embedding medium, the preparation method of described embedding medium is:
Using ultraviolet to irradiate purified water 12h, purified water temperature be warming up at 35 DEG C, the rotating speed at 700rpm/min stirs
Mix down, add thymol, stir 2min, add sodium alginate and sodium carboxymethyl cellulose, at bath temperature 55 DEG C,
Water bath sonicator 40s, bath temperature rises to 95 DEG C, adds polyvinyl alcohol 124, Liquid Macrogol, collagen protein, OK a karaoke club
Glue, polyvinylpyrrolidone, spermol, ethyl hydroxybenzoate and agar, water bath sonicator 3min, i.e. obtain frozen section embedding
Agent.
The present invention at least includes following beneficial effect: collagen protein and agar have good water solublity and transparency, simultaneously can
To increase the elasticity of section, simultaneously because himself reduced immunogenicity of collagen protein, section itself will not be interfered；
Sodium alginate has mass transfer performances, simultaneously its adhesion；Spermol its stability that can ensure that embedding medium and toughness；Oxybenzene
Ethyl ester has good antibiotic property, can effectively prevent section rotten；Thymol has good bactericidal action, prevents tissue
The rotten corruption of section.
Using substep to add component at different temperature in the preparation method of present invention design, it not only ensure that embedding medium
Homogeneity, and the appearance of the phenomenons such as caking can be prevented, use simultaneously mixing that ultrasonic method adds between each component with
And the dissolubility of component.
The embedding medium of present invention design has good heat conductivity, transparency, toughness, solubization and ductility, and it is hard
Degree is suitable.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part also will be by the present invention
Research and practice and be understood by the person skilled in the art
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, to make those skilled in the art with reference to description word
Can implement according to this.
Should be appreciated that used herein such as " have ", " comprising " and " including " term do not allot one or many
Other element individual or the existence of a combination thereof or interpolation.
Embodiment 1,
A kind of frozen section embedding medium, including:
Purified water 1000g, polyvinyl alcohol 124 80g, PVPK30 g, sodium carboxymethyl cellulose 10g and poly-
Ethylene glycol 300 50g.
Polyvinyl alcohol 124, polyvinylpyrrolidone, sodium carboxymethyl cellulose and Liquid Macrogol are analytical pure.
Embodiment 2,
A kind of frozen section embedding medium, including:
Purified water 1000g, polyvinyl alcohol 124 100g, polyvinylpyrrolidone 50g, sodium carboxymethyl cellulose 20g and poly-
Ethylene glycol 300 80g.
Polyvinyl alcohol 124, polyvinylpyrrolidone, sodium carboxymethyl cellulose and Liquid Macrogol are analytical pure.
Embodiment 3,
A kind of frozen section embedding medium, including:
Purified water 1000 parts, polyvinyl alcohol 124 90g, PVP g, sodium carboxymethyl cellulose 15g and
Liquid Macrogol 65g.
Polyvinyl alcohol 124, polyvinylpyrrolidone, sodium carboxymethyl cellulose and Liquid Macrogol are analytical pure.
Embodiment 4,
A kind of frozen section embedding medium, including:
Purified water 1000g, polyvinyl alcohol 124 90g, polyvinylpyrrolidone 35g, sodium carboxymethyl cellulose 15g and poly-
Ethylene glycol 300 60g.
Polyvinyl alcohol 124, polyvinylpyrrolidone, sodium carboxymethyl cellulose and Liquid Macrogol are analytical pure.
Embodiment 5,
The method of preparation frozen section embedding medium described in embodiment 1～5
By the quality of each component in embodiment 1～5, by purified water, polyvinyl alcohol 124, polyvinylpyrrolidone, carboxylic first
Base sodium cellulosate and Liquid Macrogol mixing, be heated to 95 DEG C, stirring, i.e. obtaining frozen section embedding medium.
Embodiment 6,
A kind of frozen section embedding medium, including:
Purified water 1000g, polyvinyl alcohol 124 90g, PVP g, sodium carboxymethyl cellulose 15g, poly-second
Glycol 300 70g, collagen protein 2g, thymol 1g, agar 1g, carrageenan 7g, sodium alginate 11g, spermol
0.3g and ethyl hydroxybenzoate 0.1g.
Preparation method:
Using ultraviolet to irradiate purified water 12h, purified water temperature be warming up at 35 DEG C, the rotating speed at 700rpm/min stirs
Mix down, add thymol, stir 2min, add sodium alginate and sodium carboxymethyl cellulose, at bath temperature 55 DEG C,
Water bath sonicator 40s, bath temperature rises to 95 DEG C, adds polyvinyl alcohol 124, Liquid Macrogol, collagen protein, OK a karaoke club
Glue, polyvinylpyrrolidone, spermol, ethyl hydroxybenzoate and agar, water bath sonicator 3min, i.e. obtain frozen section embedding
Agent.
Frozen section embedding medium of the present invention can apply to pathological diagnosis in operation to the embedding of tissue it can also be used to often
Rule pathological diagnosis, immunohistochemical diagnosis technology, diagnostic technique in molecular biology.
Although embodiment of the present invention are disclosed as above, but it is not restricted in description and embodiment listed fortune
With, it can be applied to various applicable the field of the invention completely, for those skilled in the art, and can be easily
Realizing other amendment, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention does not limit
In specific details.
Claims (5)
1. a frozen section embedding medium, it is characterised in that include the component of following mass fraction:
Purified water 1000 parts, polyvinyl alcohol 124 80～100 parts, PVPK30～50 parts, carboxymethyl cellulose
Sodium 10～20 parts and Liquid Macrogol 50～80 parts；
Purified water, polyvinyl alcohol 124, polyvinylpyrrolidone, sodium carboxymethyl cellulose and Liquid Macrogol are mixed,
It is heated to 95 DEG C, is stirring, i.e. obtain frozen section embedding medium.
2. frozen section embedding medium as claimed in claim 1, it is characterised in that include the component of following mass fraction:
Purified water 1000 parts, polyvinyl alcohol 124 90 parts, polyvinylpyrrolidone 35 parts, sodium carboxymethyl cellulose 15 parts
With Liquid Macrogol 60 parts.
3. frozen section embedding medium as claimed in claim 1 or 2, it is characterised in that polyvinyl alcohol 124, polyethylene
Ketopyrrolidine, sodium carboxymethyl cellulose and Liquid Macrogol are analytical pure.
4. frozen section embedding medium as claimed in claim 1, it is characterised in that include the component of following mass fraction:
Purified water 1000 parts, polyvinyl alcohol 124 90 parts, PVP part, sodium carboxymethyl cellulose 15 parts,
Liquid Macrogol 70 parts, collagen protein 2 parts, thymol 1 part, agar 1 part, carrageenan 7 parts, sodium alginate
11 parts, spermol 0.3 part and ethyl hydroxybenzoate 0.1 part.
5. frozen section embedding medium as claimed in claim 4, it is characterised in that the preparation method of described embedding medium is:
Using ultraviolet to irradiate purified water 12h, purified water temperature be warming up at 35 DEG C, the rotating speed at 700rpm/min stirs
Mix down, add thymol, stir 2min, add sodium alginate and sodium carboxymethyl cellulose, at bath temperature 55 DEG C,
Water bath sonicator 40s, bath temperature rises to 95 DEG C, adds polyvinyl alcohol 124, Liquid Macrogol, collagen protein, OK a karaoke club
Glue, polyvinylpyrrolidone, spermol, ethyl hydroxybenzoate and agar, water bath sonicator 3min, i.e. obtain frozen section embedding
Agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610342036.7A CN106018050A (en) | 2016-05-20 | 2016-05-20 | Frozen section embedding agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610342036.7A CN106018050A (en) | 2016-05-20 | 2016-05-20 | Frozen section embedding agent |
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| CN106018050A true CN106018050A (en) | 2016-10-12 |
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ID=57095463
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| CN201610342036.7A Pending CN106018050A (en) | 2016-05-20 | 2016-05-20 | Frozen section embedding agent |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106855475A (en) * | 2017-03-18 | 2017-06-16 | 王瑞才 | A kind of rapid tissue reagent treatment for pathological section |
| CN108037147A (en) * | 2017-11-29 | 2018-05-15 | 云南省农业科学院质量标准与检测技术研究所 | A kind of plant root freezing microtome section production method for Synchrotron Radiation X-Ray Fluorescence microanalysis |
| CN110426259A (en) * | 2019-08-14 | 2019-11-08 | 武汉赛维尔生物科技有限公司 | Polyethylene glycol is used for the application of animal tissue sections grease dyeing |
| CN110887719A (en) * | 2019-12-20 | 2020-03-17 | 云南中医药大学 | Method for processing tissue sample of histopathology |
| CN111982629A (en) * | 2020-08-25 | 2020-11-24 | 北京市农林科学院 | Ultrathin freezing slicing method for edible mushroom tissue cells |
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| CN1077795A (en) * | 1992-04-13 | 1993-10-27 | 范顺才 | Frozen tissue section embedding agent and compound method thereof |
| CN1279013A (en) * | 2000-06-27 | 2001-01-10 | 中国人民解放军北京军区总医院 | Supporting agent for frozen tissue section |
| JP2002031586A (en) * | 2000-05-06 | 2002-01-31 | Tadafumi Kawamoto | Bio-tissue freezing slicing method, slice preservation method and bio-tissue freezing slicing device |
| JP2003038172A (en) * | 2001-07-31 | 2003-02-12 | Fuji Photo Film Co Ltd | Frozen block containing protease |
| CN101375682A (en) * | 2008-10-03 | 2009-03-04 | 罗荣 | Biological tissue slice supporting agent and method for carrying quick slice in surgery using the same |
| CN101785876A (en) * | 2010-01-20 | 2010-07-28 | 南方医科大学 | Decalcification method for bone implant materials |
| CN102816401A (en) * | 2012-08-20 | 2012-12-12 | 湖南科技大学 | Embedding medium suitable for plant tissue frozen section and frozen section method |
| CN103278361A (en) * | 2013-05-16 | 2013-09-04 | 青岛科技大学 | Low-temperature embedding method of polymer thin film |
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2016
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Patent Citations (8)
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|---|---|---|---|---|
| CN1077795A (en) * | 1992-04-13 | 1993-10-27 | 范顺才 | Frozen tissue section embedding agent and compound method thereof |
| JP2002031586A (en) * | 2000-05-06 | 2002-01-31 | Tadafumi Kawamoto | Bio-tissue freezing slicing method, slice preservation method and bio-tissue freezing slicing device |
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| JP2003038172A (en) * | 2001-07-31 | 2003-02-12 | Fuji Photo Film Co Ltd | Frozen block containing protease |
| CN101375682A (en) * | 2008-10-03 | 2009-03-04 | 罗荣 | Biological tissue slice supporting agent and method for carrying quick slice in surgery using the same |
| CN101785876A (en) * | 2010-01-20 | 2010-07-28 | 南方医科大学 | Decalcification method for bone implant materials |
| CN102816401A (en) * | 2012-08-20 | 2012-12-12 | 湖南科技大学 | Embedding medium suitable for plant tissue frozen section and frozen section method |
| CN103278361A (en) * | 2013-05-16 | 2013-09-04 | 青岛科技大学 | Low-temperature embedding method of polymer thin film |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106855475A (en) * | 2017-03-18 | 2017-06-16 | 王瑞才 | A kind of rapid tissue reagent treatment for pathological section |
| CN108037147A (en) * | 2017-11-29 | 2018-05-15 | 云南省农业科学院质量标准与检测技术研究所 | A kind of plant root freezing microtome section production method for Synchrotron Radiation X-Ray Fluorescence microanalysis |
| CN110426259A (en) * | 2019-08-14 | 2019-11-08 | 武汉赛维尔生物科技有限公司 | Polyethylene glycol is used for the application of animal tissue sections grease dyeing |
| CN110887719A (en) * | 2019-12-20 | 2020-03-17 | 云南中医药大学 | Method for processing tissue sample of histopathology |
| CN111982629A (en) * | 2020-08-25 | 2020-11-24 | 北京市农林科学院 | Ultrathin freezing slicing method for edible mushroom tissue cells |
| CN111982629B (en) * | 2020-08-25 | 2023-11-17 | 北京市农林科学院 | Ultrathin frozen slicing method for edible fungus tissue cells |
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Application publication date: 20161012 |
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