CN101375682A - Biological tissue slice supporting agent and method for carrying quick slice in surgery using the same - Google Patents

Abstract

The invention relates to a supporting agent for slicing biological tissue and a method of using the supporting agent to quickly slice when surgery is conducted. The supporting agent for slicing biological tissue is butane gas. The slicing method comprises the following steps: (A) cropping the biological tissue; (B)pasting tissue; (C) spraying gas, that is, pressing a spray nozzle of a butane gas spray-can towards the pasting tissue, causing the tissue surface to be in water mist state while the spraying is conducted, pressing a ball sack to spray gas to the pasting tissue for several times, and causing the color of the tissue surface to turn from dusty pink to light grey and brown, with the pasting tissue being cooled, hardened, and in low temperature and fixation state; and (D) slicing. The invention has the advantages of evident effects of saving funds and reducing cost, simple, quick and convenient operation,, easy grasp of the method, long-term or short-term use. And the invention is economical and practical, can achieve the same effect, and has the evident social benefit and economical benefit, particularly to the promotion and use in basic (second grade) hospitals .

Description

The biological tissue section supporting agent and undergo surgery with it in the method for rapid section

Technical field

The present invention relates in the pathology technique to use butane gas and replace in the operation method of frozen section fast, specifically a kind of biological tissue section supporting agent and undergo surgery with it in the method for frozen section fast.

Background technology

As everyone knows, many case patients be unable to do without the needs of quick frozen section in the middle of operation, and its purpose mainly is in time to understand and to investigate thoroughly that lesion nature does quick frozen section for the formulation operation plan in the middle of the operation.Current high-tech developing rapidly makes high-grade Medical Devices constantly enter the medical market, purchase quick freezing microtome that a Daepori leads to regard to hospital pathology specialty speech and also need tens0000 yuan more than the hundreds of thousands unit, it is easier said than done that the suitable hospital of two first class is purchased a quick freezing microtome, general all is to afford to use and the phenomenon ubiquity that cannot afford, so, hospitals at different levels pathology specialty is purchased the Medical Devices of a top grade to clinical extremely important, and especially quick frozen section looms large more in the operation.

Summary of the invention

The purpose of this invention is to provide a kind of biological tissue section supporting agent.

Another object of the present invention provide a kind of undergo surgery with the biological tissue section supporting agent in the method for rapid section.

Biological tissue section supporting agent of the present invention is a butane gas.

The method of rapid section comprised the steps: during biological tissue section supporting agent of the present invention underwent surgery

1.5 * 1.5 * 0.5 centimetre of size, the uniform square sheet fabric texture of thickness are cut into specimens from pri in A, Xiu Qie biological tissue, repair cut smooth,

B, aerolar tissue will be repaiied then and cut smooth tablet tissue and be pasted on the wax stone clip position of paraffin slicing machine,

C, jetly push shower nozzle facing to aerolar tissue with being about to the butane gas aerosol can, make tissue surface be water smoke shape state while spraying, at this moment with sacculus facing to aerolar tissue continuously by pinch carry out under several jet, the edge injection limit makes the tissue surface color by the red light gray crineous that becomes gradually of ash, at this moment aerolar tissue is by spray gas refrigeration, hardening, be the low temperature stationary state

D, section are carried out paraffin slicing machine immediately and are made serial section, and slice thickness reaches 3-5um and gets final product.

The present invention uses butane gas to the sample that need make quick frozen section in the 311 example operations and replaces quick frozen section method to reach satisfactory effect, be analyzed with the quick freezing microtome section of the two refrigeration of CELCA cm 1900 types simultaneously, both effects of cutting into slices are approaching, and it is as shown in the table:

Butane gas compares with quick freezing microtome section situation

Chipping qualities of the present invention, Color both equally at all distinguishable clear cell nucleus of microscopically analysis and observation, cytoplasm and chromatinic ultra microstructure and tissue morphology, can reach identical effect to the pathological diagnosis quality.Using butane gas replaces the time of quick freezing microtome section can shorten about 10 minutes.

The present invention compared with prior art, its advantage: 1. the method section effect and freezing microtome section effect basically identical fast, some performance, method also are better than the section of conventional freezing microtome fast, do not need to wait for the sample refrigeration from the beginning, but continuous uninterrupted is carried out every procedure operation, also can save time; 2. invest little (cost less than hundred yuan/repacking once), instant effect, in time convenient, with to doing, being particularly suited for the suitable hospital pathology department of two first class can generally use, and is one of a kind of desirable quick frozen section method; 3. aerolar tissue is moderate through of short duration processings back hardness, be easy to the cutting thin slice (3-5um), and thickness evenly, cut into slices smooth, non-wrinkled; Section is attached on the clean glass slide securely and need not to use albumen glycerine, also need not to blot with filter paper; 5. the made section background of cutting into slices after air-dry washing does not totally have attachment, is easy to painted, the differentiation of cell; 6. Zhi Bei every section slide after the dyeing of conventional histology haematine-Yihong (HE) is pollution-free, and coloration result is clear; 7. it is clear to reach conventional H E dyeing background fast, and institutional framework, nuclear, pulp chromaticness are clearly demarcated, and reliable pathological diagnosis foundation is arranged; 8. except that carrying out conventional H E dyeing, can carry out also that various spies dye, dyeing such as SABC and immunofluorescence; 9. have good economic benefit and social benefit, be easy to promote.

Safety evaluatio: butane gas belongs to inflammable thing, is a kind of harmfulness escaping gas, certain gas is all arranged in indoor volatilization when repacking or operation, and use keeps fire away during operation.Reequip in our experience and too not near when operating, have in studio under the condition of ventilation equipment and reequip, be still safe in the short time the human body facial area.

The present invention replaces the needs of quick frozen section in the operation for pathology technique provides a kind of low consumptive material, method of operating high-effect, easy to operate, quick formula.The present invention directly on paraffin slicing machine section replace quick frozen section, and make good pathological section, clinically provide in time to give, pathological diagnosis foundation accurately and reliably.

Embodiment

The following examples can make those skilled in the art more fully understand the present invention, but do not limit the present invention in any way.

Embodiment 1

1, makes the butane gas aerosol can

1. purchase one jar of the aroma type high-quality butane gas (butane gas) that the commercial gas lighter uses; 2. commercially available spray form is pushed and is sprayed first, and how many its shower nozzle sizes is matched according to butane gas jar size and capacity, selects moderate easy fitting operation just; 3. the rubber pipette bulb that clinical laboratory is commonly used (with hand by pinching jet sacculus) is one; 4. one jar of commercially available fluid sealant.

A hole (hole size is decided according to shower nozzle size and suction pipe thickness) is made a call at the butane aerosol can top cover place of having prepared moment, push deep in the pipette nozzle patchhole with being about to spray form, promptly carry out sealing in nozzle boss bottom simultaneously, be advisable, treat to use in several minutes with gas leakage not with fluid sealant.

2, implementation and operation method and step

The specimens from pri that needs to do quick frozen section in the middle of the operation is cut into the 1.5X1.5X0.5CM size, the uniform square tablet of thickness, repair cut smooth, if (fragment not reshaping soft tissue stack smooth also can), to repair then and cut smooth tablet tissue and be pasted on the wax stone clip position of paraffin slicing machine, this moment is because of organizing moistening toughness albumen, it is Pasting, be difficult for taking off, push under the shower nozzle number facing to the tissue of pasting with the butane gas aerosol can of soon having made, make a jar interior butane gas be the vaporific tissue surface that is sprayed on stickup, make tissue surface be water smoke shape state gets final product while spraying, at this moment promptly with the rubber pipette bulb prepared facing to aerolar tissue with hand continuously by pinch carry out under several jet, the edge injection limit makes the tissue surface color by the red light gray crineous that becomes gradually of ash, at this moment aerolar tissue has made the spray gas refrigeration, hardening, be the low temperature stationary state, likeness in form wood-like, gentle slightly.Carry out paraffin slicing machine immediately and make serial section, slice thickness can reach 3-5um and get final product, and makes the section tiling not have folding, and thickness is even, and mount is waited to dye on clean slide as early as possible.Cut into slices if be difficult for when aerolar tissue reaches deliquescing in several minutes, at this moment can be repeatedly at aerolar tissue surface spray butane gas, several down carry out jet facing to aerolar tissue by pinching with rubber pipette bulb again, the aerolar tissue hardening of freezing is advisable, repeatedly serial section.If change again when another part sample is done section and can remove former aerolar tissue with haemostatic clamp or tweezers at any time, the tissue specimen of doing in addition to cut into slices is repaiied to cut again and be pasted on the paraffin slicing machine wax stone clip position after smooth, undertaken by each step of preceding method.

Claims (2)

1, a kind of biological tissue section supporting agent, it is characterized in that: it is a butane gas.

2, undergo surgery with the described biological tissue section supporting agent of claim 1 in the method for rapid section, it is characterized in that it comprises the steps:

1.5 * 1.5 * 0.5 centimetre of size, the uniform square sheet fabric texture of thickness are cut into specimens from pri in A, Xiu Qie biological tissue, repair cut smooth;

B, aerolar tissue will be repaiied then and cut smooth tablet tissue and be pasted on the wax stone clip position of paraffin slicing machine;

C, jetly push shower nozzle facing to aerolar tissue with being about to the butane gas aerosol can, make tissue surface be water smoke shape state while spraying, at this moment with sacculus facing to aerolar tissue continuously by pinch carry out under several jet, the edge injection limit makes the tissue surface color by the red light gray crineous that becomes gradually of ash, at this moment aerolar tissue by spray gas refrigeration, hardening, is the low temperature stationary state;

D, section are carried out paraffin slicing machine immediately and are made serial section, and slice thickness reaches 3-5um and gets final product.