CN106011145B - A kind of adversity gene and its coding albumen and application from jerusalem artichoke - Google Patents
A kind of adversity gene and its coding albumen and application from jerusalem artichoke Download PDFInfo
- Publication number
- CN106011145B CN106011145B CN201510581421.2A CN201510581421A CN106011145B CN 106011145 B CN106011145 B CN 106011145B CN 201510581421 A CN201510581421 A CN 201510581421A CN 106011145 B CN106011145 B CN 106011145B
- Authority
- CN
- China
- Prior art keywords
- gene
- jerusalem artichoke
- lea14
- application
- adversity gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention relates to field of biotechnology, and in particular to a kind of adversity gene LEA14 and its preparation method and application from jerusalem artichoke.The degeneration-resistant LEA14 gene base sequence of jerusalem artichoke such as SEQ ID NO1；Or, with gene of the base sequence homology 85% or more shown in SEQ ID NO:1.The amino acid sequence of LEA14 coding is as shown in SEQ ID NO 2.The present invention clones the gene order of adversity gene LEA14 using polymeric enzymatic amplification technology from jerusalem artichoke stem tuber, gene obtained can be used for Genetic Transformation in Higher Plants, plant is improved to the resistance of a variety of abiotic stress such as arid, with high salt, there is high value in plant stress-resistance Upgrading and application.
Description
Technical field
The present invention relates to field of biotechnology, and in particular to a kind of from the adversity gene LEA14 of jerusalem artichoke and its preparation
Methods and applications.
Background technique
Jerusalem artichoke is commonly called as Jerusalem artichoke or Jerusalem artichoke, is a kind of economic plants that can be planted on salt-soda soil.With other economic plants
It compares, jerusalem artichoke has the characteristics that saline-alkali tolerant, drought-enduring, cold-resistant and disease and insect resistance etc. are degeneration-resistant.The multiple resistance that jerusalem artichoke itself has
Shape keeps its potentiality to be exploited in terms of gene specific very big, studies jerusalem artichoke adversity gene, for understanding jerusalem artichoke
Degeneration-resistant mechanism and further progress genetic resources, which utilize, to be of great significance.
Late embryo stage occurs Abundant protein (Late embryogenesis abundant protein, LEA) and is reducing ring
It is played an important role in the injury of border Stress on Plant.LEA protein generally accumulates in seed development advanced stage under field conditions (factors), and
The signals such as arid, cold, with high salt and ABA can be responded, there is very strong resistivity to a variety of abiotic stress.LEA protein is logical
It crosses holding Premeabilisation of cells pressure, protection membrane structure, protect the modes such as other albumen to maintain plant normal as molecular chaperones
Metabolic response.This characteristic based on LEA protein makes plant be overexpressed endogenous or external source LEA egg by technique for gene engineering
It is white, can cultivate drought resisting, cold-resistant, salt tolerant crop varieties, in terms of plant breeding have significant application value.
Summary of the invention
The object of the present invention is to provide a kind of adversity genes and its preparation method and application from jerusalem artichoke.
A kind of adversity gene from jerusalem artichoke, the degeneration-resistant LEA14 gene base sequence of jerusalem artichoke such as SEQ ID NO 1；
Or, with gene of the base sequence homology 85% or more shown in SEQ ID NO:1.
The amino acid sequence such as SEQ ID NO 2 of the degeneration-resistant LEA14 gene coding of jerusalem artichoke.
LEA14 gene of the present invention is made of 750 nucleotide.The protein of LEA14 gene coding of the present invention is embryo's evening
Abundant protein occurs for the phase, is made of 153 amino acid.
A kind of preparation of adversity gene described in claim 1, includes the following steps:
It chooses jerusalem artichoke stem tuber extraction total serum IgE and synthesizes cDNA under the action of AMV reverse transcriptase using total serum IgE as template；
Two primers are designed, using above-mentioned cDNA as template, library primer is built in conjunction with 5 ' and carries out PCR amplification, it is degeneration-resistant to obtain jerusalem artichoke
LEA14 genetic fragment；
Above-mentioned amplified fragments recycling rear clone enters carrier T, and jerusalem artichoke adversity gene shown in SEQ ID NO 1 is obtained after sequencing
LEA14。
The primer are as follows:
3'-LEAP-1:5'-CGCACCAATGTCCTTCACCAG-3'；
3’-LEAP-2:5'-ACACCTTAACCCCCACATCTAGC-3'。
A kind of application of adversity gene, the adversity gene are preparing the application in degeneration-resistant genetically modified plants.
Application of the adversity gene in the genetically modified plants of preparation salt resistance alkaline stress.The adversity gene is anti-in preparation
Application in the genetically modified plants of drought.
It is that the present invention realizes the utility model has the advantages that
The present invention is cloned from jerusalem artichoke obtains degeneration-resistant protein coding gene LEA14, belongs to late embryo stage and Abundant protein occurs
Gene, and Gossypium orboreum LEA gene order similitude highest, but its sequence similarity is only 67%.Gene obtained can be used for
Genetic Transformation in Higher Plants, improves plant to the resistances of a variety of abiotic stress such as arid, with high salt, plant stress-resistance Upgrading with answer
There is high value in.In order to further analyze action and function of the gene in salt, Drought Stress, using the present invention
Obtain gene as it can be seen that wildtype Arabidopsis thaliana and the present invention turn LEA14 gene arabidopsis have apparent difference in resistance, turn
The arabidopsis of LEA14 gene has apparent salt resistance, drought-resistant ability than the plant of wild type, it is seen that being transferred to for LEA14 gene mentions
The high salt resistance drought-resistant ability of Arabidopsis plant.The acquisition of jerusalem artichoke LEA14 gene through the invention can be used for plant genetic and turn
Change, improves plant to the resistance of a variety of abiotic stress such as arid, with high salt, there is significant application value in terms of plant breeding.
Detailed description of the invention
Fig. 1 is that agarose electrophoresis provided in an embodiment of the present invention identifies total serum IgE product map.
Fig. 2 is agarose electrophoresis identification of cdna product map provided in an embodiment of the present invention.
Specific embodiment
The present invention is further described below by specific embodiment, it is not limit that following embodiment, which is descriptive,
Qualitatively, this does not limit the scope of protection of the present invention.
The present invention clones the gene order of adversity gene LEA14 using polymeric enzymatic amplification technology from jerusalem artichoke stem tuber, is obtained
The gene obtained can be used for Genetic Transformation in Higher Plants, improve plant to the resistance of a variety of abiotic stress such as arid, with high salt, in Genes For Plant Tolerance
There is high value in inverse Upgrading and application.
The degeneration-resistant LEA14 gene, is prepared by the following:
1. the building of jerusalem artichoke cDNA library
The present invention takes the maturity period jerusalem artichoke stem tuber of robust growth, with Tiangeng biochemical technology Co., Ltd plant Total RNAs extraction
Kit extracts total serum IgE, using total serum IgE as template, referring to Clontech company cDNA library building kit explanation, in reverse transcription
CDNA double-strand is synthesized under the action of enzyme and polymerase.
2. the design of primer
The present invention designs two 3 '-LEAP-1:5'-CGCACCAATGTCCTTCACCAG-3' of specific primer；3'-
LEAP-2:5'-ACACCTTAACCCCCACATCTAGC-3', 5 ' provided in conjunction with cDNA library building kit are built library primer and are expanded
Increase jerusalem artichoke LEA14 gene.
3. obtaining LEA14 genetic fragment using PCR method
The present invention utilizes forward and reverse primer amplification LEA14 gene order using the high quality cDNA double-strand synthesized as template.
PCR condition are as follows: 1. 95 DEG C, 4min；2. 95 DEG C, 30s；58 DEG C, 30s；72 DEG C, 1min；30 circulations；3. 72 DEG C, 10min.
4. clone identification and sequencing
After amplified fragments use hundred Imtech DNA QIAquick Gel Extraction Kit recovery purifying of Beijing, clone is connected to pMD19-T load
In body, conversion DH5 α competent cell carries out clone identification and sequencing.
5. sequence is analyzed
The present invention is analyzed by nucleotide sequencing, final to obtain anti contravariance related gene LEA14, nucleotide sequence letter
Breath can obtain the amino acid sequence of its coding as shown in SEQ ID NO 2 as shown in SEQ ID NO 1.
Adversity gene LEA14 of the present invention can be used for Genetic Transformation in Higher Plants, improve resistance (salt resistance, drought-resistant)
Application in plant.
Embodiment 1
The building of jerusalem artichoke cDNA library
1.RNA is extracted
The fresh jerusalem artichoke stem tuber tissue of 50~100mg is taken, is fully ground rapidly after liquid nitrogen is added, homogenate is moved back to 1.5mL EP
Pipe selects Tiangeng company RNA extracts kit, extracts total serum IgE.
Identify that total serum IgE, product are shown in Fig. 1 with 1% agarose gel electrophoresis.Two band of 28S and 18S rRNA is clear in figure, than
Example is about 2:1, indicates that the total serum IgE extracted is undegraded, quality is higher.
2.cDNA library construction
Using total serum IgE as template, referring to Clontech company cDNA library building kit explanation, in reverse transcriptase and polymerization
CDNA double-strand is synthesized under the action of enzyme.
With the cDNA of 1% agarose gel electrophoresis detection synthesis, product is shown in Fig. 2.CDNA, which shows, in figure is uniformly distributed
Disperse shape band, and segment be greater than 100bp, show synthesis cDNA mass it is higher.
Embodiment 2
PCR method obtains LEA14 genetic fragment
Have gene data according to species close in ncbi database, draws with 5.0 software design of Primer Premier
Object: 3 '-LEAP-1:5'-CGCACCAATGTCCTTCACCAG-3'；
3 '-LEAP-2:5'-ACACCTTAACCCCCACATCTAGC-3' are provided in conjunction with cDNA library building kit
5 ' build library primer, expand jerusalem artichoke LEA14 gene.Primer is synthesized by Shanghai biotechnology Co., Ltd.
Successively mix following reagent in the 1.5mL EP pipe of sterilizing: 10 × PCR buffer, 5.0 4 μ L of μ L, dNTPs,
1 μ L (20ng) of cDNA template, 0.5 0.5 μ L of μ L, Taq of primer, adds sterile water to be settled to 50 μ L.
PCR response procedures are as follows: 95 DEG C of initial denaturations 4min, 95 DEG C of denaturation 30s, 58 DEG C of annealing 30s, 72 DEG C of extension 1min, altogether
30 circulations are expanded, in 72 DEG C of extension 10min.
Embodiment 3
Clone identification and sequencing
1.PCR product purification and recycling
Take the UV detection after 1.0% agarose 120V electrophoresis 40min, EB dyeing of PCR product obtained by above-described embodiment
Amplified fragments cut specific band under ultraviolet device, are carried out with the DNA QIAquick Gel Extraction Kit of hundred Tyke biotech company of Beijing
Genetic fragment recycling.
2. the connection and conversion of recovery product and pMD19-T carrier
The target fragment being recovered to is connected in pMD19-T carrier, system is as follows: I 1 μ L of Solution, 5 μ L of carrier T,
4 μ L of target fragment DNA, 16 DEG C of connections overnight.
Above-mentioned 10 μ L connection product is added in DH5 α competent cell, it is soft to mix.
Ice bath 30min, 42 DEG C of water-baths heat shock 90s, ice bath 2min, 800 μ L LB liquid mediums are added, and (37 DEG C warm in advance
Bath), it mixes.
37 DEG C of shaking tables (50rpm, 15min；100rpm, 15min；150rpm, 15min) culture, 5000rpm centrifugation 3min,
800 μ L supernatants are discarded, 200 μ L of remaininging suction is blown uniformly, is coated on the LB plate containing 100 μ g/mL Amp, until bacterium solution is inhaled completely
It receives, 37 DEG C of inversion overnight incubations.
3. target fragment sequencing
Picking single bacterium is fallen in LB liquid medium of the 1mL containing 100 μ g/mL Amp, 37 DEG C, 200rpm shaken cultivation 3-
4h。
Using cultured bacterium solution as template, whether purpose is inserted into M13-F and M13-R universal primer PCR detection single colonie
Segment.Primer sequence are as follows: M13-F:
5'-GTAAAACGACGGCCAGTG-3'；M13-R:
5’-CAGGAAACAGCTATGACC-3’。
It selects the positive colony containing target fragment and is sent to the raw work in Shanghai and be sequenced.
The present invention is analyzed by nucleotide sequencing, final to obtain jerusalem artichoke adversity gene LEA14, base sequence information
As shown in SEQ ID NO 1, the amino acid sequence of coding is as shown in SEQ ID NO 2.
SEQ ID NO 1
Sequence signature:
Length: 750 bases
Type: DNA
Chain: single-stranded
Topological structure: line style
Source: jerusalem artichoke (Helianthus tuberosus Linn)
SEQ ID NO 2
Sequence signature:
Length: 153 amino acid
Type: amino acid
Chain: single-stranded
Topological structure: line style
Source: jerusalem artichoke (Helianthus tuberosus Linn)
Embodiment 4
The acquisition of transgenic Arabidopsis plants and resistance analysis
1. the building of plant expression vector and the conversion of Agrobacterium
With restriction enzyme XBa I and Sac I distinguish the enzyme enzyme site of double digestion LEA14 gene PCR product and
LEA14 segment and pCAMBIA2301 carrier is separately recovered in pCAMBIA2301 carrier, is connected with the ratio of 5:1, by PCR and
Recombinant plasmid is identified in digestion.
1) digestion system are as follows:
37 DEG C, overnight.
2) glue recycles LEA14 genetic fragment and pCAMBIA2301 carrier as described in Example 3.
3) the LEA14 segment of recycling and pCAMBIA2301 expression vector are connected overnight at 16 DEG C, linked system are as follows:
4) connection product is converted into Escherichia coli XL1-Blue, picking single colonie is inoculated in the triangular flask of 20mL LB+Km
In, 37 DEG C of constant-temperature shaking cultures are stayed overnight, and do PCR detection by template of bacterium solution.Positive bacterium colony is expanded and is cultivated, extraction plasmid is simultaneously pure
Change, does double digestion with XBa I and Sac I, whether detection clone is correct.
5) plant expression vector built is converted into Agrobacterium EHA105 using freeze-thaw method, obtains recombinational agrobacterium, and
It identifies to obtain positive transformant (transformant containing LEA14 gene shown in SEQ ID NO 1) through PCR, for infecting quasi- south
Mustard plant.
2.LEA14 the acquisition and identification of transgenic arabidopsis
Above-mentioned recombinational agrobacterium is infected to Arabidopsis plant inflorescence to be transformed, collects its seed.The seed kind that will be collected
(the 60 μ g/mL containing Km) screening T is implanted on the plate of MS solid medium0For positive transgenic plant.Green seedlings are moved into basin
Middle growth, to T0The green seedlings in generation are grown up, and are born pods and are collected seed progress T1For the screening of positive plant.The T that screening is obtained1
It is planted for positive plant, collects different T1The seed of generation positive strain.The T that will be collected1It is planted in the 60 μ g/mL's containing Km for seed
On MS solid medium, T is screened2For homozygous lines.Transgenic wheat line genome progress PCR is extracted using CTAB method to test
Card, obtains T2For transgenic arabidopsis homozygote strain.
3. the Resistance Identification of the transgenic arabidopsis of the gene containing LEA14
Transgenic arabidopsis is selfed a homozygous generation, 3 homozygous transformation plants is chosen, collects seed.After planting, seedling is raw
It is 7 days long, with control group wildtype Arabidopsis thaliana and T2For transgenic seedlings, dislocation contains the MS of 300mM NaCL and mannitol respectively
On the plate of solid medium, the growing state of 10-25 days research plant is cultivated.The results are shown in Table 1, and 70% is left after salt stress
Right nontransgenic plants are withered and dead, and the transgenic plant death rate is only 30% or so.Under drought stress, nontransgenic plants
It is slow that growth fraction turns LEA14 gene plant, T2It is significantly higher than WT lines for the fresh weight of homozygous line transgenic plant, shows to turn
Gene plant is resistant to arid compared with WT lines.
It can be seen that degeneration-resistant border function of the LEA gene under arid and salt stress, will can be used for making using transgenic technology improvement
In the research and industrialization production of object drought resistance and salt tolerance.
Growing state after the salt of 1 transgenosis of table and wildtype Arabidopsis thaliana, Osmotic treatment
| Number | Fresh weight (mg) after Osmotic treatment | Survival rate (%) after salt treatment |
| Control | 8.2 | 28.1 |
| Turn LEA14 gene strain 1 | 15.3 | 70.1 |
| Turn LEA14 gene strain 2 | 16.2 | 67.4 |
| Turn LEA14 gene strain 3 | 14.1 | 65.2 |
Claims (7)
1. a kind of adversity gene from jerusalem artichoke, it is characterised in that: the degeneration-resistant LEA14 gene base sequence of jerusalem artichoke such as SEQ ID
Shown in NO 1.
2. adversity gene described in claim 1, it is characterised in that: the amino acid sequence of the degeneration-resistant LEA14 gene coding of jerusalem artichoke is such as
Shown in SEQ ID NO 2.
3. a kind of preparation of adversity gene described in claim 1, characterized by the following steps:
The building of jerusalem artichoke cDNA library: it chooses jerusalem artichoke stem tuber and extracts total serum IgE, using total serum IgE as template, in the work of AMV reverse transcriptase
With lower synthesis cDNA；
Two primers are designed, using above-mentioned cDNA as template, library primer is built in conjunction with 5 ' and carries out PCR amplification, obtain the degeneration-resistant LEA14 of jerusalem artichoke
Genetic fragment；
Above-mentioned amplified fragments recycling rear clone enters carrier T, and jerusalem artichoke adversity gene LEA14 shown in SEQ ID NO 1 is obtained after sequencing.
4. the preparation of adversity gene as claimed in claim 3, it is characterised in that: the primer are as follows:
3'-LEAP-1: 5' -CGCACCAATGTCCTTCACCAG -3'；
3’-LEAP-2: 5'-ACACCTTAACCCCCACATCTAGC -3'。
5. the application of a kind of adversity gene described in claim 1 and carrier, it is characterised in that: the adversity gene and carrier exist
Prepare the application in degeneration-resistant genetically modified plants.
6. the application of adversity gene described in claim 5 and carrier, it is characterised in that: prepared by the adversity gene and carrier
Application in the genetically modified plants of salt resistance alkaline stress.
7. the application of adversity gene described in claim 5 and carrier, it is characterised in that: prepared by the adversity gene and carrier
Application in the genetically modified plants of drought resisting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510581421.2A CN106011145B (en) | 2015-09-14 | 2015-09-14 | A kind of adversity gene and its coding albumen and application from jerusalem artichoke |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510581421.2A CN106011145B (en) | 2015-09-14 | 2015-09-14 | A kind of adversity gene and its coding albumen and application from jerusalem artichoke |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106011145A CN106011145A (en) | 2016-10-12 |
| CN106011145B true CN106011145B (en) | 2019-07-05 |
Family
ID=57082553
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510581421.2A Active CN106011145B (en) | 2015-09-14 | 2015-09-14 | A kind of adversity gene and its coding albumen and application from jerusalem artichoke |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106011145B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110004158B (en) * | 2019-04-30 | 2021-04-27 | 四川农业大学 | Moso bamboo gene PeLEA14 and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002051978A2 (en) * | 2000-12-27 | 2002-07-04 | Institut National De La Recherche Agronomique (Inra) | Yeasts transformed by genes enhancing their cold stress tolerance |
| CN102373223A (en) * | 2010-08-27 | 2012-03-14 | 中国科学院成都生物研究所 | Nucleotide sequence of LEA3 (Late Embryogenesis Abundant 3) protein coding gene from drought-resistant H.vulgare ssp.vulgare and application thereof |
| CN103421104A (en) * | 2012-05-25 | 2013-12-04 | 中国科学院上海生命科学研究院 | Application of OsLEA3-2 in improvement of stress resistance of crop |
| CN104119430A (en) * | 2013-04-26 | 2014-10-29 | 中国科学院植物研究所 | Plant drought resistance related protein, coding gene and application thereof |
-
2015
- 2015-09-14 CN CN201510581421.2A patent/CN106011145B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002051978A2 (en) * | 2000-12-27 | 2002-07-04 | Institut National De La Recherche Agronomique (Inra) | Yeasts transformed by genes enhancing their cold stress tolerance |
| CN102373223A (en) * | 2010-08-27 | 2012-03-14 | 中国科学院成都生物研究所 | Nucleotide sequence of LEA3 (Late Embryogenesis Abundant 3) protein coding gene from drought-resistant H.vulgare ssp.vulgare and application thereof |
| CN103421104A (en) * | 2012-05-25 | 2013-12-04 | 中国科学院上海生命科学研究院 | Application of OsLEA3-2 in improvement of stress resistance of crop |
| CN104119430A (en) * | 2013-04-26 | 2014-10-29 | 中国科学院植物研究所 | Plant drought resistance related protein, coding gene and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| PREDICTED: late embryogenesis abundant protein Lea14-A [Gossypium raimondii];GenBank;《NCBI Reference Sequence: XP_012481600.1》;20150522;origin * |
| Sweetpotato late embryogenesis abundant 14 (IbLEA14) gene influences lignification and increases osmotic- and salt stress-tolerance of transgenic calli;Sung-Chul Park，et al;《Planta》;20111231;摘要，第623页右栏最后1段-第624页右栏第1段 * |
| 菊芋综合利用的研究进展;王志勇 等;《安徽农业科学》;20091231;第4节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106011145A (en) | 2016-10-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107541520B (en) | OsSAUR11 gene related to rice root development and stress resistance, coding protein and application | |
| CN107988236B (en) | Genetic engineering application of oryza sativa auxin transport protein gene OsPIN9 | |
| CN112626080B (en) | R gene for controlling soybean-rhizobium matching property, protein and application thereof | |
| WO2023065966A1 (en) | Application of bfne gene in tomato plant type improvement and biological yield increase | |
| CN113621625B (en) | Application of sesame SiERF103 gene in enhancing plant resistance | |
| CN103014035B (en) | Tumorous stem mustard stress-resistant gene, plant expression vector, construction method and application thereof | |
| CN109748959B (en) | Anthocyanin synthesis related protein SlANT1L, and coding gene and application thereof | |
| CN112048488B (en) | OsPEAMT2 gene for improving heading stage maturing rate of paddy rice under high temperature stress, protein and application thereof | |
| CN111593065B (en) | Rubber tree phytoene dehydrogenase gene VIGS silencing system and construction method and application thereof | |
| CN103183731B (en) | Dendrobe DnMYB type transcription factor, coding gene, carrier and engineering bacteria and application thereof | |
| CN105524933B (en) | OsJMJ714 influences the function and its application of rice grain size and salt stress patience | |
| CN102154337B (en) | Gossypium hirsutum mitogen-activated protein kinas 6 (GhMAPK6) gene and application thereof | |
| CN106011145B (en) | A kind of adversity gene and its coding albumen and application from jerusalem artichoke | |
| CN110982817A (en) | amiRNA for resisting wheat yellow mosaic virus and application thereof | |
| CN106939314B (en) | Halophyte drought-resistant gene Hg.S4 and application thereof | |
| CN106892973A (en) | Plant adversity resistance related protein GhMYB4 and encoding gene and application | |
| CN106011152A (en) | Transcription factor TFSALT1 for improving salt resistance of rice, and application thereof | |
| CN110643617A (en) | Rice grain weight related OsGASR9 gene, application thereof, protein, expression vector and transgenic rice method | |
| CN112225790A (en) | Rice salt stress resistance related gene ONAC103, and coding protein and application thereof | |
| CN104611335A (en) | Specific peanut promoter AhRSP and application thereof | |
| CN114591984B (en) | Application of OsAP79 gene in inducing rice to resist brown planthoppers | |
| CN113549602B (en) | Phyllostachys pubescens ascorbic acid peroxidase gene PeAPX1 and application thereof | |
| CN113173981B (en) | Phyllostachys pubescens PeDREB3 gene and application thereof in plant cold resistance regulation | |
| CN103254299B (en) | Method for acquiring anti-fungal-disease plant | |
| CN115851821B (en) | Application of BBX16 gene in improving plant salt tolerance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |