CN105998239B - Preparation method and application of Guangxi large-fruit hawthorn leaf extract - Google Patents

Preparation method and application of Guangxi large-fruit hawthorn leaf extract Download PDF

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CN105998239B
CN105998239B CN201610454260.5A CN201610454260A CN105998239B CN 105998239 B CN105998239 B CN 105998239B CN 201610454260 A CN201610454260 A CN 201610454260A CN 105998239 B CN105998239 B CN 105998239B
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filtrate
ethanol
guangxi
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concentrated solution
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CN105998239A (en
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蓝鸣生
陈路
黄健
吴无畏
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Guangxi Botanical Garden of Medicinal Plants
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

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Abstract

The invention discloses a preparation method of a Guangxi big fruit hawthorn leaf extract, which comprises the following steps: step one, extracting coarse powder of Guangxi big-fruit hawthorn leaves with ethanol, taking a first extracting solution, filtering to obtain a first filtrate, and removing the ethanol in the first filtrate to obtain a first concentrated solution; step two, adding water into the first filter residue for extracting to obtain a second extracting solution, and filtering to obtain a second filtrate; and step three, evaporating the second filtrate, adding ethanol into the second filtrate, standing to obtain supernatant and lower suspension, respectively filtering to obtain third filtrate and fourth filtrate, combining the third filtrate and the fourth filtrate, removing the ethanol to obtain second concentrated solution, mixing the second concentrated solution and the first concentrated solution, and evaporating and concentrating to obtain the compound. The preparation method is simple and feasible, is very beneficial to industrial production, and animal pharmacodynamic experiments prove that the extract produced by the preparation method contains effective components for treating hyperlipidemia, can be used for treating hyperlipidemia, and can also be used for preparing health-related products for preventing and treating hyperlipidemia.

Description

Preparation method and application of Guangxi large-fruit hawthorn leaf extract
Technical Field
The invention relates to the field of preparation methods and application of hawthorn leaf extracts, in particular to the field of preparation methods and application of a cantonese crataegus pinnatifida leaf extract.
Background
The Guangxi big fruit crataegus pinnatifida plant is the Malus pinnatifida Malus chinensis (Bois) chev. and Malus pumila Malus leiocalyca S.Z.huang, is originally recorded in the food therapy 'Qianjin food therapy' of the Tang Dynasty in China, and has an application history for more than one thousand years. It is sour and sweet in nature and warm in nature. It enters stomach and large intestine meridians. Has the functions of relieving stuffiness of the chest, promoting the production of body fluid to quench thirst, and harmonizing the middle-jiao to relieve pain. Can be used for treating phlegm, fluid retention, abdominal distention, thirst, cholera, emesis, abdominal pain, and dysentery. Modern medicine research proves that Guangxi big fruit hawthorn mainly has the following functions: the traditional Chinese medicine composition has the functions of reducing blood pressure, enhancing myocardial contractility, reducing cholesterol and triglyceride content in blood of experimental model hyperlipidemic rats, promoting mesenteric microcirculation recovery, increasing secretion of gastric juice, gastric acid and pepsin, enhancing pepsin activity, prolonging hypnotic time of hypnotic drugs, inhibiting bacteria, protecting liver, improving organism immunity, scavenging free radicals and the like, and has very low toxic and side effects. However, no research on the efficacy of Guangxi fructus crataegi leaves in treating hyperlipidemia and the extraction and preparation method of the effective components thereof is reported.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
The invention also aims to provide a preparation method of the Guangxi big fruit hawthorn leaf extract, which is simple and feasible and is very beneficial to industrial production; the invention also provides application of the extract, which contains effective components for treating hyperlipemia, can be used for treating hyperlipemia and can also be used for preparing health-related products for preventing and treating hyperlipemia.
The invention provides a preparation method of a Guangxi big fruit hawthorn leaf extract, which comprises the following steps:
step one, extracting coarse powder of Guangxi big-fruit hawthorn leaves with ethanol, taking a first extracting solution, filtering to obtain a first filtrate and a first filter residue, and removing the ethanol in the first filtrate until no alcohol smell exists to obtain a first concentrated solution; wherein the weight of the ethanol for extraction is 6-20 times of the weight of the coarse powder of the leaves of the Guangxi big fruit hawthorn;
step two, adding water into the first filter residue for extraction to obtain a second extracting solution, and filtering to obtain a second filtrate and a second filter residue, wherein the using weight of the water for extraction is 6-20 times of the weight of coarse powder of the leaves of the Guangxi big fruit hawthorn;
and step three, evaporating the second filtrate to 1/2 of the weight of the original Guangxi big fruit hawthorn leaf coarse powder, adding ethanol into the mixture until the volume fraction of the ethanol in the mixed solution reaches 60-70%, standing for 12-24 h to obtain supernatant liquid and lower suspension, respectively filtering the supernatant liquid and the lower suspension to obtain third filtrate and fourth filtrate, merging the third filtrate and the fourth filtrate, removing the ethanol in the merged filtrate until no alcohol smell exists, obtaining second concentrated solution, mixing the second concentrated solution with the first concentrated solution obtained in the step one to obtain mixed concentrated solution, and evaporating and concentrating until the relative density of the mixture reaches 1.30-1.40/80 ℃, thus obtaining the Guangxi big fruit hawthorn leaf extract.
Preferably, in the preparation method of the extract of the leaves of the hawthorn in the Guangxi province, the volume fraction of the ethanol is 60-90%.
Preferably, the preparation method of the Guangxi big fruit hawthorn leaf extract,
in the first step, ethanol is used for extraction, wherein the extraction method adopts reflux, impregnation, percolation, ultrasound or microwave;
and in the second step, adding water into the first filter residue for extraction, wherein the extraction adopts a method of decoction, reflux, immersion, percolation, ultrasound or microwave.
Preferably, the preparation method of the Guangxi big fruit hawthorn leaf extract,
recovering and removing ethanol in the first filtrate obtained in the step one and the third filtrate and the fourth filtrate obtained in the step three by reduced pressure distillation or atmospheric distillation;
and the second filtrate obtained in the second step and the mixed concentrated solution obtained in the third step are evaporated and concentrated by boiling evaporation or reduced pressure distillation concentration to remove water.
The application of the Guangxi big fruit hawthorn leaf extract is used for preparing a medicine or food for treating hyperlipidemia.
Preferably, the Guangxi fructus crataegi leaf extract is used, and auxiliary materials are added into the Guangxi fructus crataegi leaf extract to prepare tablets, sugar-coated tablets, film-coated tablets, enteric-coated tablets, buccal tablets, effervescent tablets, hard capsules, soft capsules, pills, dripping pills, granules, tea, powder, ointment, pellets, suspensions, solutions, injections, liposome preparations, suppositories, plaster, ointments, creams, sprays, drops or patches.
The invention at least comprises the following beneficial effects: the preparation method is simple and feasible, is very beneficial to industrial production, and animal pharmacodynamic experiments prove that the extract produced by the preparation method contains effective components for treating hyperlipidemia, can be used for treating hyperlipidemia, and can also be used for preparing health-related products for preventing and treating hyperlipidemia.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
Example 1
A method for preparing an extract of leaves of fructus crataegi of Guangxi province comprises:
step one, extracting coarse powder of Guangxi big-fruit hawthorn leaves with ethanol, taking a first extracting solution, filtering to obtain a first filtrate and a first filter residue, and removing the ethanol in the first filtrate until no alcohol smell exists to obtain a first concentrated solution; wherein the weight of the ethanol used for extraction is 6 times of the weight of the coarse powder of the Guangxi big fruit hawthorn leaves;
step two, adding water into the first filter residue for extraction to obtain a second extracting solution, and filtering to obtain a second filtrate and a second filter residue, wherein the using weight of the water for extraction is 6 times of the weight of coarse powder of the Guangxi big fruit hawthorn leaves;
and step three, evaporating the second filtrate to 1/2% of the weight of the original Guangxi big fruit hawthorn leaf coarse powder, adding ethanol into the mixture until the volume fraction of the ethanol in the mixed solution reaches 60%, standing for 12 hours to obtain an upper layer clear solution and a lower layer suspension, respectively filtering the upper layer clear solution and the lower layer suspension to obtain a third filtrate and a fourth filtrate, merging the third filtrate and the fourth filtrate, removing the ethanol in the merged filtrate until no alcohol smell exists to obtain a second concentrated solution, mixing the second concentrated solution with the first concentrated solution obtained in the step one to obtain a mixed concentrated solution, and evaporating and concentrating until the relative density of the mixture reaches 1.30/80 ℃ to obtain the Guangxi big fruit hawthorn leaf extract.
The application of the Guangxi big fruit hawthorn leaf extract is used for preparing a medicine or food for treating hyperlipidemia.
The Guangxi fructus crataegi leaf extract is used for preparing tablets, sugar-coated tablets, film-coated tablets, enteric-coated tablets, buccal tablets, effervescent tablets, hard capsules, soft capsules, pills, dropping pills, granules, tea, powder, paste, pellets, suspensions, solutions, injections, liposome preparations, suppositories, plasters, ointments, creams, sprays, drops or patches by adding auxiliary materials into the Guangxi fructus crataegi leaf extract.
Example 2
A method for preparing an extract of leaves of fructus crataegi of Guangxi province comprises:
step one, extracting coarse powder of Guangxi big-fruit hawthorn leaves with ethanol, taking a first extracting solution, filtering to obtain a first filtrate and a first filter residue, and removing the ethanol in the first filtrate until no alcohol smell exists to obtain a first concentrated solution; wherein the weight of the ethanol used for extraction is 12 times of the weight of the coarse powder of the leaves of the Guangxi big fruit hawthorn;
step two, adding water into the first filter residue for extraction to obtain a second extracting solution, and filtering to obtain a second filtrate and a second filter residue, wherein the using weight of the water for extraction is 12 times of the weight of coarse powder of the Guangxi big fruit hawthorn leaves;
and step three, evaporating the second filtrate to 1/2% of the weight of the original Guangxi big fruit hawthorn leaf coarse powder, adding ethanol into the mixture until the volume fraction of the ethanol in the mixed solution reaches 65%, standing for 18h to obtain an upper layer clear solution and a lower layer suspension, respectively filtering the upper layer clear solution and the lower layer suspension to obtain a third filtrate and a fourth filtrate, merging the third filtrate and the fourth filtrate, removing the ethanol in the merged filtrate until no alcohol smell exists to obtain a second concentrated solution, mixing the second concentrated solution with the first concentrated solution obtained in the step one to obtain a mixed concentrated solution, and evaporating and concentrating until the relative density of the mixture reaches 1.35/80 ℃ to obtain the Guangxi big fruit hawthorn leaf extract.
The application of the Guangxi big fruit hawthorn leaf extract is used for preparing a medicine or food for treating hyperlipidemia.
The Guangxi fructus crataegi leaf extract is used for preparing tablets, sugar-coated tablets, film-coated tablets, enteric-coated tablets, buccal tablets, effervescent tablets, hard capsules, soft capsules, pills, dropping pills, granules, tea, powder, paste, pellets, suspensions, solutions, injections, liposome preparations, suppositories, plasters, ointments, creams, sprays, drops or patches by adding auxiliary materials into the Guangxi fructus crataegi leaf extract.
Example 3
A method for preparing an extract of leaves of fructus crataegi of Guangxi province comprises:
step one, extracting coarse powder of Guangxi big-fruit hawthorn leaves with ethanol, taking a first extracting solution, filtering to obtain a first filtrate and a first filter residue, and removing the ethanol in the first filtrate until no alcohol smell exists to obtain a first concentrated solution; wherein the weight of the ethanol used for extraction is 20 times of the weight of the coarse powder of the Guangxi big fruit hawthorn leaves;
step two, adding water into the first filter residue for extraction to obtain a second extracting solution, and filtering to obtain a second filtrate and a second filter residue, wherein the using weight of the water for extraction is 20 times of the weight of coarse powder of the Guangxi big fruit hawthorn leaves;
and step three, evaporating the second filtrate to 1/2% of the weight of the original Guangxi big fruit hawthorn leaf coarse powder, adding ethanol into the mixture until the volume fraction of the ethanol in the mixed solution reaches 70%, standing for 24 hours to obtain an upper layer clear solution and a lower layer suspension, respectively filtering the upper layer clear solution and the lower layer suspension to obtain a third filtrate and a fourth filtrate, merging the third filtrate and the fourth filtrate, removing the ethanol in the merged filtrate until no alcohol smell exists to obtain a second concentrated solution, mixing the second concentrated solution with the first concentrated solution obtained in the step one to obtain a mixed concentrated solution, and evaporating and concentrating until the relative density of the mixture reaches 1.40/80 ℃ to obtain the Guangxi big fruit hawthorn leaf extract.
The application of the Guangxi big fruit hawthorn leaf extract is used for preparing a medicine or food for treating hyperlipidemia.
The Guangxi fructus crataegi leaf extract is used for preparing tablets, sugar-coated tablets, film-coated tablets, enteric-coated tablets, buccal tablets, effervescent tablets, hard capsules, soft capsules, pills, dropping pills, granules, tea, powder, paste, pellets, suspensions, solutions, injections, liposome preparations, suppositories, plasters, ointments, creams, sprays, drops or patches by adding auxiliary materials into the Guangxi fructus crataegi leaf extract.
Example 4
A method for preparing an extract of leaves of fructus crataegi of Guangxi province comprises:
step one, extracting coarse powder of Guangxi big-fruit hawthorn leaves with ethanol, taking a first extracting solution, filtering to obtain a first filtrate and a first filter residue, and removing the ethanol in the first filtrate until no alcohol smell exists to obtain a first concentrated solution; wherein the weight of the ethanol used for extraction is 6 times of the weight of the coarse powder of the Guangxi big fruit hawthorn leaves;
step two, adding water into the first filter residue for extraction to obtain a second extracting solution, and filtering to obtain a second filtrate and a second filter residue, wherein the using weight of the water for extraction is 6 times of the weight of coarse powder of the Guangxi big fruit hawthorn leaves;
and step three, evaporating the second filtrate to 1/2% of the weight of the original Guangxi big fruit hawthorn leaf coarse powder, adding ethanol into the mixture until the volume fraction of the ethanol in the mixed solution reaches 60%, standing for 12 hours to obtain an upper layer clear solution and a lower layer suspension, respectively filtering the upper layer clear solution and the lower layer suspension to obtain a third filtrate and a fourth filtrate, merging the third filtrate and the fourth filtrate, removing the ethanol in the merged filtrate until no alcohol smell exists to obtain a second concentrated solution, mixing the second concentrated solution with the first concentrated solution obtained in the step one to obtain a mixed concentrated solution, and evaporating and concentrating until the relative density of the mixture reaches 1.30/80 ℃ to obtain the Guangxi big fruit hawthorn leaf extract.
The preparation method of the Guangxi big fruit hawthorn leaf extract comprises the step of adding 60% of ethanol by volume.
The preparation method of the Guangxi big fruit hawthorn leaf extract,
in the first step, ethanol is used for extraction, wherein the extraction method adopts reflux, impregnation, percolation, ultrasound or microwave;
and in the second step, adding water into the first filter residue for extraction, wherein the extraction adopts a method of decoction, reflux, immersion, percolation, ultrasound or microwave.
The preparation method of the Guangxi big fruit hawthorn leaf extract,
recovering and removing ethanol in the first filtrate obtained in the step one and the third filtrate and the fourth filtrate obtained in the step three by reduced pressure distillation or atmospheric distillation;
and the second filtrate obtained in the second step and the mixed concentrated solution obtained in the third step are evaporated and concentrated by boiling evaporation or reduced pressure distillation concentration to remove water.
The application of the Guangxi big fruit hawthorn leaf extract is used for preparing a medicine or food for treating hyperlipidemia.
The Guangxi fructus crataegi leaf extract is used for preparing tablets, sugar-coated tablets, film-coated tablets, enteric-coated tablets, buccal tablets, effervescent tablets, hard capsules, soft capsules, pills, dropping pills, granules, tea, powder, paste, pellets, suspensions, solutions, injections, liposome preparations, suppositories, plasters, ointments, creams, sprays, drops or patches by adding auxiliary materials into the Guangxi fructus crataegi leaf extract.
Example 5
A method for preparing an extract of leaves of fructus crataegi of Guangxi province comprises:
step one, extracting coarse powder of Guangxi big-fruit hawthorn leaves with ethanol, taking a first extracting solution, filtering to obtain a first filtrate and a first filter residue, and removing the ethanol in the first filtrate until no alcohol smell exists to obtain a first concentrated solution; wherein the weight of the ethanol used for extraction is 12 times of the weight of the coarse powder of the leaves of the Guangxi big fruit hawthorn;
step two, adding water into the first filter residue for extraction to obtain a second extracting solution, and filtering to obtain a second filtrate and a second filter residue, wherein the using weight of the water for extraction is 12 times of the weight of coarse powder of the Guangxi big fruit hawthorn leaves;
and step three, evaporating the second filtrate to 1/2% of the weight of the original Guangxi big fruit hawthorn leaf coarse powder, adding ethanol into the mixture until the volume fraction of the ethanol in the mixed solution reaches 65%, standing for 18h to obtain an upper layer clear solution and a lower layer suspension, respectively filtering the upper layer clear solution and the lower layer suspension to obtain a third filtrate and a fourth filtrate, merging the third filtrate and the fourth filtrate, removing the ethanol in the merged filtrate until no alcohol smell exists to obtain a second concentrated solution, mixing the second concentrated solution with the first concentrated solution obtained in the step one to obtain a mixed concentrated solution, and evaporating and concentrating until the relative density of the mixture reaches 1.35/80 ℃ to obtain the Guangxi big fruit hawthorn leaf extract.
The preparation method of the Guangxi big fruit hawthorn leaf extract comprises the step of adding 70% of ethanol by volume.
The preparation method of the Guangxi big fruit hawthorn leaf extract,
in the first step, ethanol is used for extraction, wherein the extraction method adopts reflux, impregnation, percolation, ultrasound or microwave;
and in the second step, adding water into the first filter residue for extraction, wherein the extraction adopts a method of decoction, reflux, immersion, percolation, ultrasound or microwave.
The preparation method of the Guangxi big fruit hawthorn leaf extract,
recovering and removing ethanol in the first filtrate obtained in the step one and the third filtrate and the fourth filtrate obtained in the step three by reduced pressure distillation or atmospheric distillation;
and the second filtrate obtained in the second step and the mixed concentrated solution obtained in the third step are evaporated and concentrated by boiling evaporation or reduced pressure distillation concentration to remove water.
The application of the Guangxi big fruit hawthorn leaf extract is used for preparing a medicine or food for treating hyperlipidemia.
The Guangxi fructus crataegi leaf extract is used for preparing tablets, sugar-coated tablets, film-coated tablets, enteric-coated tablets, buccal tablets, effervescent tablets, hard capsules, soft capsules, pills, dropping pills, granules, tea, powder, paste, pellets, suspensions, solutions, injections, liposome preparations, suppositories, plasters, ointments, creams, sprays, drops or patches by adding auxiliary materials into the Guangxi fructus crataegi leaf extract.
Example 6
A method for preparing an extract of leaves of fructus crataegi of Guangxi province comprises:
step one, extracting coarse powder of Guangxi big-fruit hawthorn leaves with ethanol, taking a first extracting solution, filtering to obtain a first filtrate and a first filter residue, and removing the ethanol in the first filtrate until no alcohol smell exists to obtain a first concentrated solution; wherein the weight of the ethanol used for extraction is 20 times of the weight of the coarse powder of the Guangxi big fruit hawthorn leaves;
step two, adding water into the first filter residue for extraction to obtain a second extracting solution, and filtering to obtain a second filtrate and a second filter residue, wherein the using weight of the water for extraction is 20 times of the weight of coarse powder of the Guangxi big fruit hawthorn leaves;
and step three, evaporating the second filtrate to 1/2% of the weight of the original Guangxi big fruit hawthorn leaf coarse powder, adding ethanol into the mixture until the volume fraction of the ethanol in the mixed solution reaches 70%, standing for 24 hours to obtain an upper layer clear solution and a lower layer suspension, respectively filtering the upper layer clear solution and the lower layer suspension to obtain a third filtrate and a fourth filtrate, merging the third filtrate and the fourth filtrate, removing the ethanol in the merged filtrate until no alcohol smell exists to obtain a second concentrated solution, mixing the second concentrated solution with the first concentrated solution obtained in the step one to obtain a mixed concentrated solution, and evaporating and concentrating until the relative density of the mixture reaches 1.40/80 ℃ to obtain the Guangxi big fruit hawthorn leaf extract.
The preparation method of the Guangxi big fruit hawthorn leaf extract is characterized in that the volume fraction of the ethanol is 90%.
The preparation method of the Guangxi big fruit hawthorn leaf extract,
in the first step, ethanol is used for extraction, wherein the extraction method adopts reflux, impregnation, percolation, ultrasound or microwave;
and in the second step, adding water into the first filter residue for extraction, wherein the extraction adopts a method of decoction, reflux, immersion, percolation, ultrasound or microwave.
The preparation method of the Guangxi big fruit hawthorn leaf extract,
recovering and removing ethanol in the first filtrate obtained in the step one and the third filtrate and the fourth filtrate obtained in the step three by reduced pressure distillation or atmospheric distillation;
and the second filtrate obtained in the second step and the mixed concentrated solution obtained in the third step are evaporated and concentrated by boiling evaporation or reduced pressure distillation concentration to remove water.
The application of the Guangxi big fruit hawthorn leaf extract is used for preparing a medicine or food for treating hyperlipidemia.
The Guangxi fructus crataegi leaf extract is used for preparing tablets, sugar-coated tablets, film-coated tablets, enteric-coated tablets, buccal tablets, effervescent tablets, hard capsules, soft capsules, pills, dropping pills, granules, tea, powder, paste, pellets, suspensions, solutions, injections, liposome preparations, suppositories, plasters, ointments, creams, sprays, drops or patches by adding auxiliary materials into the Guangxi fructus crataegi leaf extract.
Example 7
Pulverizing 1[ Taiwan Chinese pear-leaved crabapple Malus doumeri (Bois) Chev ] leaf of Guangxi large-fruit hawthorn, sieving with a 20-mesh sieve, performing reflux extraction with 80% ethanol for 2 times, adding 10 times of alcohol for the first time, performing reflux extraction for 2 hours, adding 6 times of alcohol for the second time, performing reflux extraction for 1.5 hours, filtering an alcohol extract, recovering ethanol from a filtrate until no alcohol smell exists, collecting and refrigerating for later use; adding 8 times of water into filter residues, boiling for two times, adding 6 times of water into the filter residues for two times, boiling for 1 hour, filtering a water extracting solution, reducing the pressure of filtrate, evaporating and concentrating until the total amount is 1/2 of the weight of the medicinal materials, adding 90% ethanol into a concentrated solution to ensure that the alcohol content of the solution reaches 70%, uniformly stirring, standing for 12 hours, filtering and collecting an ethanol precipitation solution, recovering ethanol until no alcohol smell exists, mixing the remaining concentrated solution with the ethanol extraction concentrated solution while hot, uniformly stirring and mixing, and continuously evaporating and concentrating until the relative density is 1.35/80 ℃, thereby obtaining the extract 1.
Example 8
Pulverizing leaves of fructus crataegi 2 (Malus leiocaulica S.Z.Huang) of Guangxi fructus, sieving with 20 mesh sieve, extracting with 80% ethanol by reflux for 2 times, adding 10 times of alcohol for the first time, extracting for 2 hr by reflux, adding 6 times of alcohol for the second time, extracting for 1.5 hr by reflux, filtering the extractive solution, recovering ethanol from the filtrate until no alcohol smell exists, collecting, and refrigerating; adding 8 times of water into filter residues, boiling for two times, adding 6 times of water into the filter residues for two times, boiling for 1 hour, filtering a water extracting solution, reducing the pressure of filtrate, evaporating and concentrating until the total amount is 1/2 of the weight of the medicinal materials, adding 90% ethanol into a concentrated solution to ensure that the alcohol content of the solution reaches 70%, uniformly stirring, standing for 12 hours, filtering and collecting an ethanol precipitation solution, recovering ethanol until no alcohol smell exists, mixing the remaining concentrated solution with the ethanol extraction concentrated solution while hot, uniformly stirring and mixing, and continuously evaporating and concentrating until the relative density is 1.35/80 ℃, thereby obtaining the extract 2.
Pharmacodynamic experiment method for treating rat hyperlipidemia by Guangxi big fruit hawthorn leaf extract
Experimental methods
2.1 establishment of rat model for hyperlipemia
2.1.1 animal grouping and preliminary testing
96 male rats are randomly divided into 8 groups (normal control, model, positive 1 (simvastatin), positive 2 (simvastatin + Xuezhikang), extract 1 high dose, extract 1 low dose, extract 2 high dose and extract 2 low dose) and 12 rats in each group (the grouping conditions are shown in table 1), after each group is quantitatively fed with basal feed for 7 days, the weight is weighed, the fasting is not forbidden for 16 hours, after 10% chloral hydrate local anesthesia, cardiac puncture is carried out to collect blood, serum is separated, and four indexes of total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein blood fat are detected, the result is shown in table 2 below
TABLE 1 weight of each group before molding
Figure BDA0001024952140000091
TABLE 2 serum lipids of each group before molding
Figure BDA0001024952140000092
2.1.2 rat model establishment of hyperlipidemia
From the next day after the completion of the test, the rats in the normal group were gavaged with 10ml/kg of distilled water containing 10% tween-80 every morning, and the rest groups were gavaged with 10ml/kg of the formula emulsion every morning; the basal diet was given to the rats simultaneously in each group for free feeding. Each group is continuously perfused for 2 weeks, after 2 weeks, rats are fasted and are not forbidden to be watered for 16 hours, after 10% chloral hydrate is locally anesthetized, the heart is punctured to take blood, serum is separated, and four indexes of total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein are detected; the results are given in Table 3/4 below
TABLE 3 weight of each group after molding
Figure BDA0001024952140000101
Comparison of normal control group: 0.01 < (R) >*p＜0.05，**p＜0.01；
TABLE 4 successful modeling of serum blood lipids in each group
Figure BDA0001024952140000102
Comparison with normal control group: 0.01 < (R) >*p＜0.05，**p＜0.01；
Preparing high-fat milk:
high fat emulsion formula (100ml)
Lard oil Cholesterol Propylthiouracil Tween-80 Pig bile salt
20g 6g 0.2g (20ml propylthiouracil liquor) 10ml 2g
The preparation method of the high fat milk comprises the following steps:
1) taking 10 (50 mg/tablet) propylthiouracil tablets, adding 50ml purified water to fully dissolve the propylthiouracil tablets so as to obtain 10mg/ml propylthiouracil liquid medicine;
2) weighing lard oil in a beaker, heating and stirring the lard oil by using a magnetic stirrer to melt the lard oil, weighing cholesterol and the salt of the lard oil for 6-8 times, adding the cholesterol and the salt of the lard oil into the beaker, weighing a proper amount of tween-80, fully stirring and emulsifying the mixture evenly, finally adding 20ml of thiouracil propionate liquid medicine, adding purified water to the scale, fully stirring and emulsifying the mixture evenly, and cooling the mixture to below about 40 ℃ so as to be used for gastric lavage. After the stomach is filled, the mixture is refrigerated and stored in a refrigerator, when in use, the mixture is dissolved in water at 50 ℃ and heated fully, stirred uniformly and emulsified, and then used after the temperature is moderate.
2.2 grouping and administration
Feeding rats in cages, quantitatively feeding basic feed and water to a normal control group, and feeding purified water with the same amount to the rats through gastric lavage; except for the stomach-filling administration of the corresponding drugs, the other groups were quantitatively fed with high-fat diet and water (grouping and administration schedule are shown in table 5), the weights were measured every 3 days, the animals of each group were fasted and not prohibited after 4 weeks, and the animals of each group were treated after 12 hours. Adopting cardiac puncture to take blood, then centrifugally separating serum, and detecting four indexes of total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein blood fat; the results are given in Table 6/7 below:
table 5 grouping and dosing regimens
Figure BDA0001024952140000121
TABLE 6 body weights of groups before last dose
Figure BDA0001024952140000122
Comparison with normal control group: 0.01 < (R) >*p＜0.05，**p is less than 0.01; comparison with model groups: 0.01 < (R) >#p＜0.05，##p＜0.01.
TABLE 7 serum lipids for each group after the last dose
Figure BDA0001024952140000131
Comparison with normal control group: 0.01 < p < 0.05, p < 0.01; comparison with model groups: 0.01 < (R) >#p＜0.05，##p＜0.01.
From the above results, it can be seen that:
compared with the rats in the normal control group, the cholesterol of each group is increased compared with that of the normal group; after drug treatment, cholesterol of the hyperlipidemic rats of each administration group is reduced to different degrees. Compared with the model group, the cholesterol in the high-dose and low-dose groups of the extract 1 and the extract 2 has statistical significance, and the reduction degree of the high-dose group and the low-dose group of the extract 1 and the extract 2 is more obvious.
Compared with normal control rats, the triglyceride level of each group is increased, and the triglyceride of the hyperlipemia rats of each administration group is reduced to different degrees after the drug treatment. Compared with the model group, the data of the positive 2 groups, the extractum 1 and 2 high dose groups and the extractum 1 and 2 low dose groups for reducing triglyceride have statistical significance; wherein, the reduction degree of the high-dose groups of the extractum 1 and the extractum 2 is more obvious.
Compared with the normal control group rats, the serum LDL concentration level of each group is increased, and after the drug treatment, the serum LDL concentration level of each administration group is reduced to different degrees. Compared with the model group, the data of the reduction of the concentration of the low-density lipoprotein in the serum of each administration group has statistical significance, wherein the reduction degree of the positive 2 group, the extract 1 group and the extract 2 high-dose group is more obvious.
Compared with a normal control group, the effect of the medicines of each group in the experiment on the content of the high-density lipoprotein in the serum of the rat is small.
From the above experiments, it was confirmed that the extracts of the leaves of the wide-west crabapple (Malus pumila) thus prepared in example 7 and the leaves of the wide-west crabapple (Malus leiocalyca s.z. huang) thus prepared in example 8 all have the effect of reducing the concentrations of total cholesterol, triglyceride and low-density lipoprotein in the serum of hyperlipidemic rats.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.

Claims (3)

1. A preparation method of a Guangxi big fruit hawthorn leaf extract is characterized by comprising the following steps:
crushing Guangxi big-fruit hawthorn leaves, sieving the crushed leaves with a 20-mesh sieve, performing reflux extraction for 2 times by using 80% ethanol, adding 10 times of ethanol for the first time, performing reflux extraction for 2 hours, adding 6 times of ethanol for the second time, performing reflux extraction for 1.5 hours, filtering an ethanol extract, recovering ethanol from a filtrate until no ethanol smell exists, collecting and refrigerating the filtrate for later use; adding 8 times of water into filter residues, boiling for two times, adding 6 times of water into the filter residues for two times, boiling for 1 hour, filtering a water extracting solution, reducing the pressure of filtrate, evaporating and concentrating the filtrate until the total amount is 1/2 of the weight of the medicinal materials, adding 90% ethanol into the concentrated solution to ensure that the alcohol content of the solution reaches 70%, uniformly stirring, standing for 12 hours, filtering and collecting an ethanol precipitation solution, recovering ethanol until no alcohol smell exists, mixing the remaining concentrated solution with the ethanol extraction concentrated solution while hot, uniformly stirring and mixing, continuously evaporating and concentrating until the relative density is 1.35/80 ℃, and obtaining an extract, namely the extract of the hawthorn leaves of Guangxi fructus crataegi.
2. The use of the extract of crataegus pinnatifida bunge leaves as claimed in claim 1, for preparing a medicament for treating hyperlipidemia.
3. The use of the extract of crataegus pinnatifida bunge as claimed in claim 2, wherein the extract of crataegus pinnatifida bunge is added with auxiliary materials and made into tablets, hard capsules, soft capsules, pills, granules, teas, powders, ointments, pellets, suspensions, solutions, injections, liposome preparations, suppositories, plasters, ointments, creams, sprays, drops or patches.
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