CN105998082A - Effective part capable of resisting respiratory syncytial viruses in grubs and preparation method - Google Patents
Effective part capable of resisting respiratory syncytial viruses in grubs and preparation method Download PDFInfo
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- CN105998082A CN105998082A CN201610558453.5A CN201610558453A CN105998082A CN 105998082 A CN105998082 A CN 105998082A CN 201610558453 A CN201610558453 A CN 201610558453A CN 105998082 A CN105998082 A CN 105998082A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
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- Insects & Arthropods (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention belongs to a traditional Chinese medicament active ingredient production and extraction process, and relates to a novel process for extracting and separating an effective part capable of resisting respiratory syncytial viruses in grubs and a novel use in the field of pharmacy, in particular to extraction and preparation of an effective part capable of resisting respiratory syncytial viruses in grubs in an antivirus tracking manner. The process specifically comprises the following steps: performing reflux extraction on the grubs by virtue of distilled water, 75 percent ethanol and ethyl acetate, performing water extraction and alcohol precipitation, performing alcohol extraction and water precipitation, carrying out antivirus experiments, dissolving precipitates, treating D101, DM130, X-5, AB-8 and HPD100 macroporous resins, loading a sample on the macroporous resins, performing gradient elution at different concentrations in a manner that every 200 milliliters are connected to a bottle and elution gradient elution at each concentration is performed to a colorless state, and carrying out antivirus experiments on each eluent to determine the effective part. According to the process, the effective part capable of resisting the respiratory syncytial viruses in the grubs is found by an antivirus tracking method, and has better resisting effects on the respiratory syncytial viruses.
Description
Technical field
The present invention relates to active ingredient of Chinese herbs and produce extraction process, relate to new approaches new method that Holotrichia diomphalia Bates antivirus effective position extracts and at the new application of pharmaceutical field, particularly relate to the effective site of the mode extraction separation and purification Holotrichia diomphalia Bates anti respiratory syncytial virus that antiviral is followed the trail of.
Background technology
The most all receiving the invasion of various virus, such as SARS virus, H1N1 etc., the outburst of virus all can cause fear and the death of the mankind every time.So finding suitable antiviral drugs extremely urgent.
Respiratory syncytial virus is to cause one of most important pathogen of infant acute lower respiratory infection, also can cause interstitial pneumonia and bronchiolitis etc. that old people and immunodeficiency patient can also be caused to be in hospital simultaneously and pneumonia is dead.
Respiratory syncytial virus just received much concern before 50 years, but still lacked its effective prophylactic treatment measure at present.Ribavirin is the antiviral drugs having certain curative effect to respiratory syncytial virus of wide spectrum.But, research finds, cotton rat model through respiratory syncytial virus infection occurs in that the resistant strain of ribavirin, and patient and medical worker there are is genotoxic potential, so only occurring serious rsv infection patient is just to use this medicine, therefore explore the novel substance to respiratory syncytial virus is inhibited required by the epoch.
Holotrichia diomphalia Bates has long applicating history in traditional medicine, can cure mainly erysipelas, corneal nebula, carbuncle anal fistula with removing blood stasis with potent drugs blood, reducing swelling and alleviating pain, improving eyesight.Modern pharmacology research show, Holotrichia diomphalia Bates have treatment ophthalmic, antitumor, protect the liver, antibacterial effect.
Holotrichia diomphalia Bates has certain therapeutical effect to respiratory syncytial virus, and experiment shows that the effective site that Holotrichia diomphalia Bates is extracted has good therapeutical effect to respiratory syncytial virus.
Summary of the invention
The technical problem to be solved is the effective site that specify that Holotrichia diomphalia Bates anti respiratory syncytial virus, and provides a kind of mode utilizing antiviral to follow the trail of for the separation and Extraction of this effective site and prepare the new method of Holotrichia diomphalia Bates antivirus effective position.This effective site is extracted isolated from Scarabaeidae animal Holotrichia diomphalia Bates and be may be used for the treatment of respiratory syncytial virus infection.The method can be relatively simple effective above position is carried out separation and Extraction.
It is an advantage of the invention that, the present invention has isolated in Holotrichia diomphalia Bates the effective site with anti respiratory syncytial virus first, and invented a kind of antiviral follow the trail of method prepared by this effective site, this separation method purposiveness is strong, simply save substantial amounts of manpower and materials, be better than traditional system and separate.The effective site prepared has preferable therapeutical effect to respiratory syncytial virus.
The precipitation that effective site is water extract-alcohol precipitation of Holotrichia diomphalia Bates anti respiratory syncytial virus of the present invention, the preparation method of this effective site, comprise the steps:
(1) a certain amount of Holotrichia diomphalia Bates medical material is taken respectively with the water of 10 times, 75% ethanol of 10 times, the ethyl acetate heating and refluxing extraction 2 times of 8 times, each 2h.Merge the extracting solution concentrating under reduced pressure recycling design of twice, be concentrated into the medical material that every milliliter of medicinal liquid contains 1 gram, then do the test of In Vitro Anti respiratory syncytial virus, find that the effect of Holotrichia diomphalia Bates water extract anti respiratory syncytial virus is best.
(2) by add after the water extract in (1) ethanol regulation ethanol concentration be 70%, stand 24 hours.It is filtrated to get precipitation and supernatant.
(3) supernatant is reclaimed ethanol, by precipitation water dissolution, carry out Antiviral breeding respectively.The antiviral effect of experiment display precipitation is preferable.
(4) certain D101, X-5, DM130, AB-8, HPD100 macroporous adsorbent resin, soak with ethanol with 95% are taken respectively 24 hours, fill post afterwards, it is washed till without alcohol taste with distilled water, then it is rinsed soaking 3 hours with the aqueous hydrochloric acid solution of 5 times of column volumes 5%, afterwards with distilled water punching to PH equal to 7, it is rinsed soaking 3 hours with the NaOH aqueous solution of the 5% of 5 times of column volumes again, afterwards with distilled water punching to PH equal to 7, standby.
(5) take the precipitation in (3) and be splined on the above-mentioned five kinds of macroporous resin column handled well respectively, respectively with water, 25% ethanol, 50% ethanol, 75% ethanol, 95% ethanol is rinsed, in each eluent, every 200ml connects one bottle, and changes next eluent after being flushed to not have color again.
(6) the above-mentioned sample received is carried out Antiviral breeding.The precipitation of experiment display Holotrichia diomphalia Bates water extract-alcohol precipitation No. 1 No. 2 antiviral effects of water after D101 macroporous resin column fine.
Detailed description of the invention
Example 1(1) weigh a certain amount of Holotrichia diomphalia Bates and add 10 times amount heating and refluxing extraction twice, each 2h, merge twice extracting solution, be evaporated to the medical material that every milliliter of medicinal liquid contains 1 gram, the concentration adding ethanol regulation solution ethanol is 70%, stands 24 hours.
(2) the alcohol deposit fluid decompression sucking filtration (1) obtained, is precipitated, precipitation is used distilled water ultrasonic dissolution.
(3) solution that (2) obtain being splined on the D101 macroporous adsorbent resin handled well, is rinsed with water, every 200ml connects one bottle, rinses to colourless.
(4) solution obtained in (3) carrying out antiviral study in vitro, experimental result display No. 1 No. 2 its antiviral effects of water are preferable.
Claims (6)
1. a Holotrichia diomphalia Bates has the effective site of anti respiratory syncytial virus effect and the process of a kind of mode this effective site of extraction separation and purification utilizing antiviral to follow the trail of, after the extraction of each step separates, antiviral to be carried out tracks out the place of effective site, first with water extract-alcohol precipitation, next utilizes the effective site of D101 macroporous resin isolated Holotrichia diomphalia Bates antiviral.
The most according to claim 1, extraction separation method, it is characterised in that the solid-liquid ratio of Holotrichia diomphalia Bates water reflux, extract, is 1:10, extraction time is twice, and extraction time is each 2h.
The most according to claim 1, extraction separation method, it is characterised in that the concentrated solution concentration of Aqueous extracts is the medical material that 1ml medicinal liquid contains 1g, alcohol precipitation concentration is 70%, and the precipitate with ethanol time is 24 hours.
Extraction separation method the most according to claim 1, it is characterised in that carry out the sucking filtration that reduces pressure after precipitate with ethanol, be precipitated and supernatant, then carry out Antiviral breeding and be precipitated as antivirus effective position.
The most according to claim 1, extraction separation method, it is characterised in that macroporous resin model used is D101, eluent is water, 25% ethanol, 50% ethanol, 75% ethanol, 95% ethanol, and every 200ml connects one bottle, and the punching of each gradient is to colourless.
The most according to claim 1, extraction separation method, it is characterised in that eluent is carried out Antiviral breeding, obtains 1, No. 2 is effective site.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610558453.5A CN105998082A (en) | 2016-07-16 | 2016-07-16 | Effective part capable of resisting respiratory syncytial viruses in grubs and preparation method |
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| CN201610558453.5A CN105998082A (en) | 2016-07-16 | 2016-07-16 | Effective part capable of resisting respiratory syncytial viruses in grubs and preparation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108743626A (en) * | 2018-07-10 | 2018-11-06 | 周长征 | A kind of active ingredient and preparation method of Phellinus moderate resistance RSV viruses |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101780239A (en) * | 2009-01-15 | 2010-07-21 | 颜平安 | Four-herb anti-tumor and anti-AIDS soft capsules and preparation process thereof |
| CN104803851A (en) * | 2015-02-26 | 2015-07-29 | 周长征 | Mint effective ingredient capable of resisting respiratory syncytial virus and preparation method |
-
2016
- 2016-07-16 CN CN201610558453.5A patent/CN105998082A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101780239A (en) * | 2009-01-15 | 2010-07-21 | 颜平安 | Four-herb anti-tumor and anti-AIDS soft capsules and preparation process thereof |
| CN104803851A (en) * | 2015-02-26 | 2015-07-29 | 周长征 | Mint effective ingredient capable of resisting respiratory syncytial virus and preparation method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108743626A (en) * | 2018-07-10 | 2018-11-06 | 周长征 | A kind of active ingredient and preparation method of Phellinus moderate resistance RSV viruses |
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Application publication date: 20161012 |
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