CN105997000A - Raman spectrum detection device based on fibrescope and implementation method of detection device - Google Patents
Raman spectrum detection device based on fibrescope and implementation method of detection device Download PDFInfo
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- A61B1/06—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
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Abstract
The invention relates to a Raman spectrum detection device based on a fibrescope and an implementation method of the detection device. The detection device comprises a dual-wavelength laser device, a fiber optical Raman probe, the fibrescope, a white-light cold light source, an image pick-up device, a Raman spectrometer and a display device, wherein the white-light cold light source is connected to an optical interface of the fibrescope; the image pick-up device is arranged at the upper part of the fibrescope and is used for collecting images in the fibrescope; an output end of the image pick-up device is connected to the display device and is used for displaying the images in the fibrescope; an output end of the dual-wavelength laser device is connected to an input end of the fiber optical Raman probe; and an output end of the fiber optical Raman probe is connected to the Raman spectrometer and a detector thereof. The detection device disclosed by the invention is applicable to real-time Raman spectrum detection and analysis on living bodies of intra-luminal tissues of a human body.
Description
Technical field
The present invention relates to fibrescope field, particularly relate to a kind of Raman spectrum based on fibrescope detection dress
Put and its implementation.
Background technology
Cancer is the disease of a kind of serious threat human health, it has also become human diseases associated death first cause." China
Tumor statistics annual report " display: the annual new cancer cases of China is 3,500,000, has 2,500,000 because of cancer mortality.Controlling in cancer
More, in rate, current developed country has reached 65%, and China only has about 25%.
On the cure rate of cancer, current developed country has reached 65%, and China only has about 25%, the cure rate of cancer with
The stage that cancer finds is closely related, if finding in early days, its cure rate will be greatly improved.But many cancer patients are in early days
And non-evident sympton, very difficult discovery.Such as nasopharyngeal carcinoma.Fibrescope is a kind of conventional medical apparatus and instruments, through the tract of human body, or
Person is that the little otch that does of underwent operative enters human body intracavity, observes its intracavity lesion, determines its position, scope, can carry out operation and
Shooting, is the reliable tools of diagnosis and treatment, is widely applied clinically.
But, existing medical fibre endoscopy examining system mostly depend on conventional white light reflecting fibres endoscope (as
Fibrescope, electronic fiber endoscope etc.) observe the morphology pathological changes of cancer, during diagnosis, only according to the perusal of doctor, knot
Closing personal experience, judge tissue abnormalities portion structure and shape and identify, small lesion tissue is likely difficult to observe
Arrive, thus greatly reduce the rate of examining out, cause and fail to pinpoint a disease in diagnosis or mistaken diagnosis, there be difficulties involved when in the clinical diagnosis of cancer in early days.
Raman spectrum is a kind of inelastic scattering spectrum, it is possible to obtain the fingerprints such as the molecular structure of material abundance, vibration mode, functional group
Information need not the preparation of samples process of complexity, and Water Proton divides interference little, to protein, nucleic acid, phospholipid and sugar
The advantages such as biochemical component change is very sensitive, can be widely applied to the analysis of biomolecular structure, are a kind of lossless, quick, high
The optical detective technology of sensitivity.
Current research shows, not only the finger print region (200～2000cm of Raman spectrum-1, fingerprint) and to human body
The disease of tissue has diagnostic significance, its high wave number district (2600～3500cm-1, high wavenumber) Raman spectrum also
Have and highly important Biochemical Information can be provided.Therefore, when carrying out tissue Raman spectrum and checking, obtain fingerprint simultaneously
The Raman spectrum in district and high wave number district is the most necessary.But, for obtaining the Raman spectrum of bigger spectral region, conventional method is
Use the detector of larger area, to receive the raman spectral signal of wider wave-number range.The shortcoming of this way is to add
The volume of Raman detection system, too increases the cost of system simultaneously, is unfavorable for miniaturization and the popularization and application on a large scale of system.
On the other hand, the endoceliac apparatus of people is entered through fibrescope biopsy channel, as touched because of misoperation
Mucosa, even makes mucosa damaged and hemorrhage, " passes through body in whole or in part according to the classifying rules of " medical apparatus classification rule "
Table invades human body, the medical apparatus and instruments at the positions such as contact inner tissue, blood circulation, central nervous system " for invading apparatus,
It is i.e. invasive, then belongs to the apparatus having material risk.Human body is not made thereby it is ensured that enter people's endoceliac viewing instruments
Become damage particularly significant.A kind of feasible way is that the built-in length of apparatus endoceliac to people to be entered is any limitation as, and i.e. increases
Add spacing tissue, it is to avoid touch tissue because of the misoperation of personnel.
Summary of the invention
In view of this, it is an object of the invention to provide a kind of Raman spectrum detecting device based on fibrescope and in fact
Existing method, it is intended to be applicable to the live body of human body intraluminal tissue, the detection of real-time Raman spectrum and the device of diagnosis.
The present invention uses below scheme to realize: a kind of Raman spectrum detecting device based on fibrescope, including double wave
Long laser instrument, Raman fiber optic probe, fibrescope, white light cold light source, camera head, Raman spectrometer and data process with
Display device；Described white light cold light source is connected with the optical interface of described fibrescope, and described camera head is arranged at fiber
Endoscope top, in order to gather the image in described fibrescope, the outfan of described camera head processes with described data
It is connected with display device, in order to show the image in described fibrescope；The outfan of described dual laser is with described
The input of Raman fiber optic probe is connected；The outfan of described Raman fiber optic probe and described Raman spectrometer and detector thereof
It is connected.
Further, described Raman fiber optic probe is Y-shaped, including the first branch and the second branch, described first branch
Including an excitation fiber, described second branch includes that some are collected optical fiber, and the middle part of described laser fiber is some with described
Root is collected the middle part of optical fiber and is closed Shu Weiyi root conjunction bundle optical fiber.
Further, the described bundle optical fiber end face in detection end that closes collects optical fiber around described excitation fiber by some
Making circumferential arrangement, detection end uses metal sleeve to fix.
Further, the conjunction bundle optical fiber of described Raman fiber optic probe is provided with an adjustable spacing dress near bifurcation
Putting, described stopping means enters the length of fibrescope biopsy channel and carries out spacing in order to regulate fibre-optical probe, described spacing
Device includes fixing sleeve and fixed screw, and described fixed cover jacket casing is put on the surface of described Raman fiber optic probe, described
Fixed screw is vertically installed on described fixing sleeve, in order to be fixed described Raman fiber optic probe.
Further, described excitation fiber and described collection optical fiber all use polymeric material to wrap up.
Further, the fiber end face of the detection end of described Raman fiber optic probe is provided with plated film, described excitation fiber
End face on be coated with and allow the low pass film that passes through of two wavelength exciting lights, the end face of described collection optical fiber is coated with end sharp
The high pass film that Raman diffused light that is luminous and that allow wavelength bigger passes through.
Further, the output optical fibre of described dual laser swashing by filtering assembly and described Raman fiber optic probe
Luminous fibre is connected, in order to alternately to export the exciting light of two kinds of different wave lengths；The collection optical fiber of described Raman fiber optic probe and filter
Optical assembly connects, and its output light is connected with Raman spectrometer and detector thereof through collecting optical fiber.
Further, the exciting light of described dual laser alternately two kinds of different wave lengths of output includes 785nm exciting light
With 690nm exciting light；Described 785nm exciting light in order to complete the detection of finger print region Raman spectrum, described 690nm exciting light in order to
Complete the detection of high wave number district Raman spectrum.
Further, described fibrescope is the fibrescope meeting human body intracavity different parts, including nasopharyngeal endoscope,
Cystoscope, metroscope, vocal cords arthroscope and Uretero-kidney mirror.
Further, described white light cold light source is 300W xenon short-act lamp white light cold light source.
Further, described data process with display device is a PC.
The present invention also uses following methods to realize: the realization side of a kind of Raman spectrum detecting device based on fibrescope
Method, it is characterised in that: comprise the following steps:
Step S1: described Raman fiber optic probe stretches into the biopsy channel of described fibrescope, uses stopping means regulation light
Fine probe enters the length of described biopsy channel, described fibrescope is stretched into human body intracavity and detects；
Step S2: open white light cold light source, described camera head is arranged at fibrescope top, gathers described fiber endoscopic
Image in mirror；The outfan of described camera head is processed with described data and is connected with display device, in order to show described fibre
Image in dimension endoscope；
Step S4: open described dual laser, and send the exciting light of two kinds of different wave lengths, described exciting light passes through laser
Optical fiber input, collects Raman scattering optical transport described in collecting fiber and, to described Raman spectrometer and detector thereof, completes finger print region
The detection of Raman spectrum and the detection of high wave number district Raman spectrum.
Further, in the people's live body tissues of nasopharyngeal carcinoma obtained and the Raman spectral characteristics of normal nasopharyngeal tissue,
In finger print region: normal and tumor tissues Raman spectrum all 851,943,1004,1096,1124,1265,1316,1450,
1621 and 1660cm-1There is obvious Raman peaks at place；Compared to normal structure, the finger print region Raman spectrum characteristic of tissues of nasopharyngeal carcinoma is also
There occurs that significantly change, i.e. peak value at 851,943,1096,1124 there occurs decline, and 1004,1265,1316,
1450,1621 and 1660cm-1The peak value at place there occurs rising；The shape of the spectrum of normal structure and tumor tissues is at 1120-
1360 cm-1And 1560-1680cm-1Interval there is also significantly difference.
In high wave number district: normal and tissues of nasopharyngeal carcinoma Raman spectrum is all at 2854,2940 and 3009 cm-1With 3067 cm-1
There is obvious Raman peaks at place；Compared to normal structure, the high wave number district Raman spectrum characteristic of tissues of nasopharyngeal carcinoma also there occurs substantially
Change, i.e. compared to normal structure, tissues of nasopharyngeal carcinoma spectrum is at 2854,2940 cm-1The peak value at place there occurs rising.
Compared with prior art, present invention have the advantage that the Raman light based on fibrescope that the present invention sets up
Spectrum detection device, uses the fibre-optical probe of particular design, can enter people's body cavity easily by the biopsy channel of fibrescope
In, carry out the raman spectroscopy measurement of human body intraluminal tissue；Use exciting of dual laser alternately two kinds of different wave lengths of output
As exciting light, coordinate filtering assembly and control software, it is achieved that completing fingerprint with the spectroscopic detector of same less area
Detect while district and high wave number district Raman spectrum.It addition, the present invention can to realize human body intraluminal tissue lossless, real-time, efficient
Raman spectrum detects；Being prevented effectively from the fibre-optical probe damage to human body mucosal tissue during clinical examination, Raman spectrum is examined
The advantages such as examining system has wave number wide coverage, and volume is little, thus the analyzing and diagnosing lossless, quick for biological tissue is provided with
The Clinical detection instrument of effect, has important using value.Meanwhile, the Raman based on fibrescope that the present invention sets up is utilized
Spectrum detection device and detection method, it is thus achieved that people's live body tissues of nasopharyngeal carcinoma and the finger print region of normal nasopharyngeal tissue and high wave number
The Raman spectral characteristics in district and difference between the two thereof.
Accompanying drawing explanation
Fig. 1 is Raman spectrum detecting device based on fibrescope.
Fig. 2 is the structure chart of fibre-optical probe in Fig. 1.
Fig. 3 is that the structure chart of stopping means in Fig. 2 fibre-optical probe uses schematic diagram.
Fig. 4 is the plated film schematic diagram of end of probe fiber end face in Fig. 2 fibre-optical probe.
Fig. 5 is the normal portions nasopharynx part tissue average Raman spectrum and nasopharyngeal carcinoma group obtained under 785nm excitation
Knit lower wave number average Raman spectrum comparison diagram.
Fig. 6 is the normal portions nasopharynx part tissue average Raman spectrum and nasopharyngeal carcinoma group obtained under 690nm excitation
Knit high wave number average Raman spectrum comparison diagram.
Detailed description of the invention
Below in conjunction with the accompanying drawings and embodiment the present invention will be further described.
The present embodiment provides a kind of Raman spectrum detecting device based on fibrescope, as it is shown in figure 1, include dual wavelength
Laser instrument, Raman fiber optic probe, fibrescope, white light cold light source, camera head, Raman spectrometer and data process with aobvious
Showing device；Described white light cold light source is connected with the optical interface of described fibrescope, and described camera head is arranged in fiber
Sight glass top, in order to gather the image in described fibrescope, the outfan of described camera head and described data process with
Display device is connected, in order to show the image in described fibrescope；The outfan of described dual laser draws with described
The input of graceful fibre-optical probe is connected；The outfan of described Raman fiber optic probe and described Raman spectrometer and detector phase thereof
Connect.
In the present embodiment, described Raman fiber optic probe is Y-shaped, including the first branch and the second branch, and described first
Branch includes that an excitation fiber, described second branch include that some are collected optical fiber, and the middle part of described laser fiber is with described
The some middle parts collecting optical fiber are closed Shu Weiyi root and are closed bundle optical fiber.This fibre-optical probe can by the biopsy channel of fibrescope just
Enter human body intracavity promptly, carry out the raman spectroscopy measurement of human body intraluminal tissue.
In the present embodiment, some collection optical fiber are excited by the described bundle optical fiber end face in detection end that closes around described
Circumferential arrangement made by optical fiber, and detection end uses metal sleeve to fix, to guarantee the smooth and firm of the end face of probe portion.
In the present embodiment, that bifurcation is provided with one is adjustable spacing for the conjunction bundle optical fiber of described Raman fiber optic probe
Device, described stopping means enters the length of fibrescope biopsy channel and carries out spacing in order to regulate fibre-optical probe, described limit
Position device includes fixing sleeve and fixed screw, and described fixed cover jacket casing is put on the surface of described Raman fiber optic probe, institute
State fixed screw to be vertically installed on described fixing sleeve, in order to described Raman fiber optic probe to be fixed.By described limit
Position device can be according to detection needs, and regulation fibre-optical probe enters the length of endoscopic biopsy passage and carries out spacing, it is ensured that optical fiber is visited
Head is when detecting through biopsy channel entrance intracavity, and the end face of its end of probe does not contact the endoceliac mucosal tissue of people, keeps away
The medical-risks such as hemorrhage, the infection having exempted from the damage to intracavity mucosal tissue and cause；And can be by cone rubber sleeve to light
Fine probe is fixed, and rolled by fibre-optical probe during preventing from measuring, mobile etc. causes the deviation measuring some position, improves optical fiber
The probe positioning precision to focus.
In the present embodiment, described excitation fiber and described collection optical fiber all use polymeric material to wrap up.
In the present embodiment, Fig. 4 is fibre-optical probe end of probe plated film schematic diagram, the detection end of described Raman fiber optic probe
Fiber end face is provided with plated film, and the end face of described excitation fiber is coated with the low pass film allowing two wavelength exciting lights to pass through, with
Reduce the interference that measurement is caused by exciting light through the non-excitation light irradiation that the optical elements such as excitation fiber produce to tissue；Described
Collect and be coated with to end exciting light on the end face of optical fiber and high pass film that the Raman diffused light that allows wavelength bigger passes through, to subtract
Few exciting light is reflected into the collection optical fiber interference to tissue Raman signal through organize.
In the present embodiment, the output optical fibre of described dual laser is by filtering assembly and described Raman fiber optic probe
Excitation fiber be connected, in order to alternately to export the exciting light of two kinds of different wave lengths；The collection optical fiber of described Raman fiber optic probe
Being connected with filtering assembly, its output light is connected with Raman spectrometer and detector thereof through collecting optical fiber.
In the present embodiment, the exciting light of described dual laser alternately two kinds of different wave lengths of output includes that 785nm swashs
Luminescence and 690nm exciting light；Described 785nm exciting light in order to complete the detection of finger print region Raman spectrum, described 690nm exciting light
In order to complete the detection of high wave number district Raman spectrum.Described dual laser coordinates filtering assembly and controls software, it is achieved that
Finger print region Raman spectrum (200～2000cm is completed with the spectroscopic detector of same less area-1, fingerprint) and high
Wave number district (2600～3500cm-1, high wavenumber) detect while Raman spectrum, effectively reduce the volume of system
And reduce design cost.
In the present embodiment, described fibrescope is the fibrescope meeting human body intracavity different parts, including nose
Pharyngoscope, cystoscope, metroscope, vocal cords arthroscope and Uretero-kidney mirror.
In the present embodiment, described white light cold light source is 300W xenon short-act lamp white light cold light source.
In the present embodiment, described data process with display device is a PC.
In the present embodiment, this device can realize the detection of human body intraluminal tissue Raman spectrum lossless, real-time, efficient；Have
Effect avoids the fibre-optical probe damage to human body mucosal tissue during clinical examination, and Raman spectrum detection system has wave number and covers
Lid scope is wide, the advantages such as volume is little, thus lossless, the quick diagnosis for biological tissue provides effective Clinical detection instrument, tool
There is important using value.
In the present embodiment, described fibrescope can use fiberoptic nasopharyngoscope, and the internal diameter of its biopsy channel is 2.2mm,
Fibre-optical probe closes bundle segment outer radius 1.6mm, and during use, the conjunction bundle part of fibre-optical probe enters nose by nasopharyngeal endoscope biopsy channel
Chamber, and arrive near nasopharyngeal tissue.Conjunction bundle part at fibre-optical probe is provided with adjustable stopping means near bifurcation, its with
Fibre-optical probe close bundle part by fastening screw fix, when fibre-optical probe is during stretching into endoscope, stopping means with
Fibre-optical probe is toward close at the upper end open of biopsy channel, when stopping means arrives biopsy channel opening part, and can be by spacing dress
Put the opening part being embedded in biopsy channel, and limit fibre probe continues to move toward human body intracavity.
In the present embodiment, Y-shaped fibre-optical probe and and dual laser, detector, stopping means between company
Connect relation as in figure 2 it is shown, use schematic diagram for the structure chart of stopping means in fibre-optical probe in figure 3.The upper left dotted line of Fig. 3
Frame is the stopping means on fibre-optical probe and the partial schematic diagram of the position relationship between endoscopic biopsy feeder connection 3.Described
The stopping means of Raman fiber optic probe 2 includes fixing sleeve 5 and the fixed screw 4 of described fixing sleeve；Described fixing sleeve 5 is
Quality of rubber materials into a cone shape, to guarantee to be fixed Raman fiber optic probe 2 securely, due to optical fiber during preventing from measuring
Probe rolls, the mobile measurement error caused；Described fixing sleeve can move freely on Raman fiber optic probe 2 surface, and
The length coordinating fixed screw 4 that Raman fiber optic probe 2 is entered endoscope 1 accurately regulates, and according to concrete testing conditions
Carry out reasonable adjusting to meet various test request.
In the present embodiment, the live body intraluminal tissue Raman spectrum detecting device of the fibrescope described in employing, respectively
Normal and nasopharyngeal carcinoma biological tissue are carried out Raman spectrum test, and test spectral is as shown in Figure 5 and Figure 6.Fig. 5 is to utilize 785nm
The finger print region normal portions nasopharynx part tissue average Raman spectrum that laser excitation is measured and tissues of nasopharyngeal carcinoma average Raman spectrum.
As far as we know, this is the raman spectral signal recording nasopharyngeal carcinoma biological tissue lower wave number first.By contrast, although normal with
There is similarity in tumor tissues Raman spectrum, all occur in that 851,943,1004,1096,1124,1265,1316,1450,
1621 and 1660cm-1Raman peaks；But simultaneously it was also found that compared to normal structure, some spectral characteristic of tissues of nasopharyngeal carcinoma is also
There occurs significantly change, the such as peak value at 851,943,1096,1124 there occurs decline；And 1004,1265,
1316,1450,1621 and 1660cm-1The peak value at place there occurs rising.In addition, the spectrum of normal structure and tumor tissues
Shape is at 1120-1360 cm-1And 1560-1680cm-1Interval there is also significantly difference.Owing to Raman peaks belongs to respectively
In specific biochemical substances, therefore the Strength Changes of Raman peak position shows the development along with nasopharyngeal carcinoma, and some in tissue is biochemical
Composition there occurs specific change.The content of the albumen such as such as tryptophan, phenylalanine, tyrosine there occurs change, in addition certain
The structure of a little albumen also there occurs change.It addition, the content of nucleic acid, lipid, glucide also there occurs corresponding change.These
The significance change explanation of spectrum, the nasopharyngeal carcinoma live body Raman system that we build can detect that the specificity of tissues of nasopharyngeal carcinoma becomes
Change, be expected to realize the lossless In vivo detection of nasopharyngeal carcinoma.Fig. 6 is the high wave number district normal structure utilizing 690nm laser excitation to be measured
Average Raman spectrum with tissues of nasopharyngeal carcinoma.This is also the raman spectral signal recording nasopharyngeal carcinoma biological tissue high wave number first,
Normal and tissues of nasopharyngeal carcinoma therefrom be can be observed and can obtain 2854,2940 and 3009 cm at high wavenumber range-1With 3067
cm-1Etc. Raman peaks, and difference difference between the two: compared to normal structure, tissues of nasopharyngeal carcinoma spectrum is at 2854,2940 cm-1The peak value at place there occurs rising.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modify, all should belong to the covering scope of the present invention.
Claims (10)
1. a Raman spectrum detecting device based on fibrescope, it is characterised in that: include dual laser, Raman light
Fine probe, fibrescope, white light cold light source, camera head, Raman spectrometer and data process and display device；Described in vain
Light cold light source is connected with the optical interface of described fibrescope, and described camera head is arranged at fibrescope top, in order to
Gathering the image in described fibrescope, the outfan of described camera head processes with described data and is connected with display device,
In order to show the image in described fibrescope；The outfan of described dual laser is defeated with described Raman fiber optic probe
Enter end to be connected；The outfan of described Raman fiber optic probe is connected with described Raman spectrometer and detector thereof.
A kind of Raman spectrum detecting device based on fibrescope the most according to claim 1, it is characterised in that: institute
Stating Raman fiber optic probe is Y-shaped, and including the first branch and the second branch, described first branch includes an excitation fiber, institute
Stating the second branch and include that some are collected optical fiber, bundle is closed with the described some middle parts collecting optical fiber in the middle part of described laser fiber
It is one and closes bundle optical fiber；Some collection optical fiber are made by the described bundle optical fiber end face in detection end that closes around described excitation fiber
Circumferential arrangement, detection end uses metal sleeve to fix；Described excitation fiber and described collection optical fiber all use polymeric material
Parcel.
A kind of Raman spectrum detecting device based on fibrescope the most according to claim 2, it is characterised in that: institute
The conjunction bundle optical fiber stating Raman fiber optic probe is provided with an adjustable stopping means near bifurcation, and described stopping means is in order to adjust
Joint fibre-optical probe enters the length of fibrescope biopsy channel and carries out spacing, described stopping means include fixing sleeve with
Fixed screw, described fixed cover jacket casing is put on the surface of described Raman fiber optic probe, and described fixed screw is vertically installed in described
On fixing sleeve, in order to described Raman fiber optic probe is fixed.
A kind of Raman spectrum detecting device based on fibrescope the most according to claim 1, it is characterised in that: institute
The fiber end face of the detection end stating Raman fiber optic probe is provided with plated film, and the end face of described excitation fiber is coated with permission two
The low pass film that wavelength exciting light passes through, the end face of described collection optical fiber is coated with end exciting light and allow wavelength bigger
The high pass film that Raman diffused light passes through.
A kind of Raman spectrum detecting device based on fibrescope the most according to claim 1, it is characterised in that: institute
The output optical fibre stating dual laser is connected by the excitation fiber of filtering assembly with described Raman fiber optic probe, in order to hand over
Exciting light for two kinds of different wave lengths of output；The collection optical fiber of described Raman fiber optic probe is connected with filtering assembly, and it exports light
It is connected with Raman spectrometer and detector thereof through collecting optical fiber.
A kind of Raman spectrum detecting device based on fibrescope the most according to claim 1, it is characterised in that: institute
The exciting light stating dual laser alternately two kinds of different wave lengths of output includes 785nm exciting light and 690nm exciting light；Described
785nm exciting light is in order to complete the detection of finger print region Raman spectrum, and described 690nm exciting light is in order to complete high wave number district Raman light
The detection of spectrum.
A kind of Raman spectrum detecting device based on fibrescope the most according to claim 1, it is characterised in that: institute
Stating fibrescope is the fibrescope meeting human body intracavity different parts, including nasopharyngeal endoscope, cystoscope, metroscope, vocal cords
Arthroscope and Uretero-kidney mirror.
A kind of Raman spectrum detecting device based on fibrescope the most according to claim 1, it is characterised in that: institute
Stating white light cold light source is 300W xenon short-act lamp white light cold light source.
9. an implementation method for Raman spectrum detecting device based on fibrescope according to claim 1, it is special
Levy and be: comprise the following steps:
Step S1: described Raman fiber optic probe stretches into the biopsy channel of described fibrescope, uses stopping means regulation light
Fine probe enters the length of described biopsy channel, described fibrescope is stretched into human body intracavity and detects；
Step S2: open white light cold light source, described camera head is arranged at fibrescope top, gathers described fiber endoscopic
Image in mirror；The outfan of described camera head is connected with described display device, within showing described fibrescope
Image；
Step S3: open described dual laser, and send the exciting light of two kinds of different wave lengths, described exciting light passes through laser
Optical fiber input, collects Raman scattering optical transport described in collecting fiber and, to described Raman spectrometer and detector thereof, completes finger print region
The detection of Raman spectrum and the detection of high wave number district Raman spectrum.
The implementation method of a kind of Raman spectrum detecting device based on fibrescope the most according to claim 1, it is special
Levy and be: when to use described fibrescope be nasopharyngeal endoscope, described Raman spectrometer and detector acquisition thereof are to people's live body nasopharynx
In the Raman spectral characteristics of cancerous tissue and normal nasopharyngeal tissue,
In finger print region, normal and tumor tissues Raman spectrum all 851,943,1004,1096,1124,1265,1316,1450,
1621 and 1660cm-1There is obvious Raman peaks at place；Compared to normal structure, the finger print region Raman spectrum characteristic of tissues of nasopharyngeal carcinoma exists
851, the peak value at 943,1096,1124 there occurs decline, and 1004,1265,1316,1450,1621 and 1660cm-1Place
Peak value there occurs rising；The shape of the spectrum of normal structure and tumor tissues is at 1120-1360 cm-1And 1560-1680cm-1
Interval there is also significantly difference；
In high wave number district, normal and tissues of nasopharyngeal carcinoma Raman spectrum is all at 2854,2940 and 3009 cm-1With 3067 cm-1Place has
Significantly Raman peaks；Compared to normal structure, the high wave number district Raman spectrum characteristic of tissues of nasopharyngeal carcinoma is at 2854,2940 cm-1Place
Peak value there occurs rising.
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| PCT/CN2016/085988 WO2017206201A1 (en) | 2016-05-30 | 2016-06-16 | Fiberscope-based raman spectrum inspection device and method for implementing same |
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| CN105997000B (en) | 2023-04-14 |
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