CN105994086B - Zebra fish behavior test system capable of simulating real scene through controllable projection - Google Patents
Zebra fish behavior test system capable of simulating real scene through controllable projection Download PDFInfo
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- CN105994086B CN105994086B CN201610537092.6A CN201610537092A CN105994086B CN 105994086 B CN105994086 B CN 105994086B CN 201610537092 A CN201610537092 A CN 201610537092A CN 105994086 B CN105994086 B CN 105994086B
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Abstract
The invention provides a zebra fish behavior test system capable of simulating real scenes through controllable projection, and relates to the field of basic research of medicine and pharmacology. The behavior test system of the invention comprises: an infrared light source; the object to be detected can be placed between the infrared light source and the infrared camera; the infrared light source is used for projecting infrared light to an object to be measured; the infrared camera is used for shooting an infrared image including an object to be measured. The behavior testing system can obtain the infrared image of the object to be tested by adopting the infrared light source irradiation and infrared light source collection modes, thereby eliminating the influence of the background image, obtaining clear motion trail of the object to be tested by utilizing the change of the infrared image caused by the shielding of the object to be tested on infrared light, and improving the accuracy of behavior trail collection of the object to be tested.
Description
Technical Field
The invention relates to the field of medical and pharmacological basic research, in particular to a zebra fish behavior test system capable of simulating real scenes through controllable projection.
Background
The zebra fish is a small subtropical freshwater fish, is used as a model organism of vertebrates, has organ systems and physiological functions which are highly similar to those of mammals, has 85 percent of high homology of genomes with human beings, can be used as a substitute model in pharmacological tests, and provides important data for new drug research and mechanism research. The zebra fish is very sensitive to external environment change perception, changes in cognition, emotion and the like can be generated, and complex behavioral response is further shown, so that the zebra fish has high research significance in the field of neuropsychiatric behavior research, and the technical difficulty in how to scientifically research the relationship between scene change and behavioral response is high.
When video acquisition and analysis technology is adopted to research the influence of environmental changes on animal behaviors, a common method is to play scene pictures on one side of a test box by using a computer screen, record videos of animals and analyze behaviors in real time or at a later stage. The method adopts a playing entity such as a computer screen, so that the background interference of the coaxially oppositely acquired videos cannot be removed by behavior analysis software due to the continuous change of the background, and the accurate tracking of the action track of the target animal cannot be realized.
Disclosure of Invention
The invention aims to provide a scheme for improving the accuracy of behavior track acquisition of an object to be detected.
According to an aspect of the present invention, there is provided a behavior testing system, comprising: an infrared light source; the object to be detected can be placed between the infrared light source and the infrared camera; the infrared light source is used for projecting infrared light to an object to be measured; the infrared camera is used for shooting an infrared image including an object to be measured.
Optionally, the method further comprises: and the playing device is positioned between the object to be detected and the infrared light source and is used for playing the image towards the direction of the object to be detected.
Optionally, the playing device comprises a projector and a diffusion plate; the diffusion plate is positioned between the object to be detected and the infrared light source; the projector is positioned on the side surface of the area between the diffusion plate and the object to be measured and is used for projecting images to the diffusion plate.
Optionally, the projector is a short-focus projector, and is connected to the edge of the diffusion plate through a bracket and extends toward the object to be measured.
Optionally, the short focus projector is a reflective projector.
Optionally, the infrared light source includes an infrared lamp bead light source plate, and is located on the opposite side of the diffusion plate relative to the object to be measured.
Optionally, the diffusion plate is a box panel of one side of the light diffusion box facing the object to be measured, the light diffusion box is located on the side face of the object to be measured, and the infrared lamp bead source plate is located in the light diffusion box and can project infrared light to the object to be measured through the light diffusion box.
Optionally, the infrared camera includes an infrared filter lens and a camera, and a light transmission band of the infrared filter lens matches with a band of the infrared light source.
Optionally, the infrared light source includes a first infrared light source and a second infrared light source, and the infrared camera includes a first infrared camera and a second infrared camera; the object to be detected is positioned between the first infrared light source and the first infrared camera and between the second infrared light source and the second infrared camera; the projection directions of the infrared light of the first infrared light source and the second infrared light source are mutually vertical.
Optionally, the method further comprises: the first playing device is positioned between the object to be measured and the first infrared light source and can play images towards the object to be measured.
Optionally, the method further comprises: and the second playing device is positioned between the object to be detected and the second infrared light source and can play images towards the object to be detected.
Optionally, the method further comprises: and the image processing device is connected with the infrared camera and used for determining the motion condition of the object to be detected on the imaging plane of the infrared image according to the infrared image.
Optionally, the method further comprises: and the host is connected with the playing device and provides images.
Optionally, the wavelength of the infrared light is 930-950 nm.
Optionally, the image acquisition speed of the infrared camera is not lower than 25 frames/second.
The behavior testing system can obtain the infrared image of the object to be tested by adopting the infrared light source irradiation and infrared image acquisition modes, thereby eliminating the influence of the background image, obtaining clear motion trail of the object to be tested by utilizing the change of the infrared image caused by the shielding of the object to be tested on infrared light, and improving the accuracy of motion trail acquisition of the object to be tested.
According to another aspect of the present invention, there is provided a behavior testing method, including: projecting infrared rays from the side surface of the object to be measured to the object to be measured by using an infrared light source; and shooting an infrared image of the area of the object to be measured by an infrared camera on the opposite side of the object to be measured relative to the infrared light source.
Optionally, the method further comprises: and playing an image to the direction of the object to be detected through a playing device between the object to be detected and the infrared light source.
Optionally, the playing device comprises a projector and a diffusion plate; the playing the image to the direction of the object to be measured through the playing device comprises: and projecting an image to the diffusion plate through a projector, wherein the diffusion plate is positioned between the object to be measured and the infrared light source, and the projector is positioned on the side surface of the area between the diffusion plate and the object to be measured.
Optionally, capturing an infrared image of the region of the object to be measured by an infrared camera on the opposite side of the object to be measured with respect to the infrared light source includes: filtering light rays by using an infrared filtering lens on the opposite side of the object to be measured relative to the infrared light source, wherein the light transmitting waveband of the infrared filtering lens is matched with the waveband of the infrared light source; and shooting the infrared image filtered by the infrared filter lens through a camera.
Optionally, the projecting the infrared light to the object to be measured from the side of the object to be measured by using the infrared light source includes: projecting infrared rays to the object to be measured from two mutually vertical side surfaces of the object to be measured by utilizing two infrared light sources; the method for shooting the infrared image of the area of the object to be measured through the infrared camera on the opposite side of the object to be measured relative to the infrared light source comprises the following steps: and respectively shooting the infrared images of the area of the object to be measured on the opposite sides of the object to be measured relative to the two infrared light sources.
Optionally, the method further comprises: and playing an image to the direction of the object to be detected through a playing device between the object to be detected and one or two infrared light sources.
Optionally, the method further comprises: and determining the motion condition of the object to be detected on the imaging plane of the infrared image according to the infrared image.
By the method, the infrared image of the object to be detected can be obtained by adopting the infrared light source irradiation and infrared image acquisition modes, so that the influence of the background image can be eliminated, the clear motion trail of the object to be detected can be obtained by utilizing the change of the infrared image caused by the shielding of the object to be detected on the infrared light, and the accuracy of the motion trail acquisition of the object to be detected is improved.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
FIG. 1 is a schematic diagram of one embodiment of a behavioral testing system of the present invention.
FIG. 2 is a schematic diagram of another embodiment of a behavioral testing system of the present invention.
Fig. 3 is a schematic diagram of an embodiment of a playing device of the behavior testing system according to the present invention.
FIG. 4 is a schematic view of one embodiment of a light diffusion box of the behavioral testing system of the present invention.
FIG. 5 is a schematic diagram of a behavior testing system according to another embodiment of the present invention.
FIG. 6 is a schematic diagram of yet another embodiment of a behavioral testing system of the present invention.
FIG. 7 is a flow chart of one embodiment of a behavioral testing method of the present invention.
FIG. 8 is a flow chart of another embodiment of a behavioral testing method of the present invention.
FIG. 9 is a flow chart of yet another embodiment of a behavioral testing method of the present invention.
FIG. 10 is a flow chart of yet another embodiment of a behavioral testing method of the present invention.
Detailed Description
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
A schematic diagram of one embodiment of a behavioral testing system of the present invention is shown in fig. 1. Wherein 2 is an infrared light source, 3 is an infrared camera, and the object 1 to be measured is positioned between the infrared light source 2 and the infrared camera 3. The infrared light source 2 projects infrared light 4 to the object 1 to be measured. The infrared camera 3 shoots an infrared image towards the direction of the object 1 to be detected, and the infrared image comprises the object 1 to be detected. The object 1 to be tested may be an animal, such as zebrafish, located in a behavioral test box. In one embodiment, the uniform infrared light reaching the object to be measured can be uniform infrared light 4 emitted by the infrared light source 2, or the infrared light emitted by the infrared light source 2 can reach the object to be measured after being homogenized, which is beneficial for the infrared camera to capture uniform infrared images.
By adopting the behavior testing system, the infrared image comprising the object to be tested can be obtained by adopting the infrared light source irradiation and infrared image acquisition modes, so that the influence of the background image can be eliminated, the clear motion track of the object to be tested is obtained by utilizing the change of the infrared image caused by the shielding of the object to be tested on infrared light, and the accuracy of behavior track acquisition of the object to be tested is improved.
At present, when a behavior test is carried out in an experiment, in order to avoid the influence of a background image, only an X-direction playing scene and a Y-direction video acquisition mode are adopted to acquire animal behaviors (the X direction and the Y direction are vertical to each other). For the zebra fish aquatic animals, the influence of scene change on animal behaviors cannot be objectively evaluated due to the lack of analysis data of X-direction swimming behaviors, and the authenticity and the reliability of experimental results are seriously influenced. The behavior testing system shown in fig. 1 can realize the playing of an X-direction scene and the acquisition of an X-direction video, the data of the scene playing direction cannot be lost, the influence of the played scene as a background image on the track extraction can be eliminated, the accuracy of animal behavior analysis is improved, the requirement of the neuro-psycho-behavioral research is met, and the behavior testing system is favorable for application in the fields of pharmacology and neuro-biological research.
A schematic diagram of another embodiment of the behavioral testing system of the present invention is shown in fig. 2. On the basis of the behavior testing system shown in fig. 1, a playing device 5 is further included. The playing device 5 is located between the infrared light source 3 and the object 1 to be measured, and the infrared light 4 can irradiate on the object 1 to be measured through the playing device 5.
By adopting the behavior testing system, the playing device can be used for playing the image and observing the reaction of the object to be tested to the image. Because infrared light can see through play device, the mode that adopts infrared light source to shine and infrared light source to gather obtains the infrared image including the object that awaits measuring, can get rid of the image that plays static or change in the play device and cause the influence to the image that the camera was gathered to can confirm the movement track of the object that awaits measuring according to infrared image, provide the data basis for the analysis of the object that realizes awaiting measuring to the scene reaction.
In one embodiment, the structure of the playing device 5 is shown in fig. 3. Here, 51 is a diffusion plate, and 52 is a projector. The projector 52 is located on the side of the region between the diffusion plate 51 and the object 1, and projects an image on the side of the diffusion plate 51 facing the object 1.
The behavior testing system plays images in a mode that the projector is matched with the diffusion plate, ensures that infrared light can penetrate through the diffusion plate to reach an object to be tested, and ensures that the infrared images collected by the infrared camera can reflect the motion trail of the object to be tested. The projector is arranged on the side face of the area between the diffusion plate and the object to be tested, so that infrared light is not shielded by the projector, strong light emitted by a bulb of the projector cannot interfere with the behavior of the object to be tested, and the accuracy of behavior test is improved.
In one embodiment, the projector may be connected to the edge of the diffusion plate by a bracket 53, and extend toward the object to be measured, so as to prevent the projector from blocking the image played by the playing device and prevent the projector from blocking the infrared light. In one embodiment, the support 53 is a retractable support, which facilitates flexible adjustment of the positional relationship between the projector and the diffuser plate as desired.
In one embodiment, the projector 52 is a short focus projector capable of projecting an image of a predetermined size on the diffuser plate 51 over a short distance. In one embodiment, the short focus projector needs to ensure that the projected area is not less than 80cm x 80cm within a distance of 20 cm. By adopting the projector, the requirement on the distance between the diffusion plate and the object to be detected can be reduced, the size of the behavior detection system is reduced, the object to be detected can be ensured to see the played image in a short distance, and the influence of the image on the object to be detected is ensured.
In one embodiment, a reflective projector is used to project an image onto the diffuser plate 51. Because the short-focus projection of fisheye formula can produce image distortion, adopt reflective projector can provide better image and present the image quality, improve the degree of accuracy of behavioral testing.
In one embodiment, the infrared light source 2 may be an infrared lamp bead light source board, and is located on the opposite side of the playing device 5 relative to the object 1 to be measured, and projects infrared light 4 toward the object 1 to be measured. The infrared light source has dispersed and uniform light, can make the infrared image even, and is beneficial to the analysis of the track of the object to be detected. Particularly, after the infrared light 4 passes through the diffusion plate 51, the infrared light 4 is further homogenized, and the degree of homogenization of the infrared image is further improved. In one embodiment, the size of the infrared lamp bead light source plate can be about 48cm by 48cm, the infrared lamp bead light source plate is composed of 100 LED infrared lamp beads which are arranged at equal intervals, 10 LED lamp beads are connected in series to form a group, and the groups are connected in parallel. Such dimensions are advantageous for behavioural testing of objects to be tested, such as zebrafish.
In one embodiment, the infrared light source 2 emits light at a wavelength of 930nm to 950nm, preferably 940 nm. Meanwhile, the infrared camera is provided with a 940nm narrow-band infrared high-transmittance filter. Because the infrared light bead red storm phenomenon with the wavelength of 940nm is weaker, a clearer infrared image can be obtained, and a better test effect is achieved.
In one embodiment, as shown in fig. 4, 6 is a light diffusion box, and the object 1 to be measured is located between the light diffusion box 6 and the infrared camera 3. The side of the light diffusion box 6 facing the object 1 to be measured is a diffusion plate 51. The light diffusion box 6 is internally provided with an infrared light source 2, the infrared light source 2 can be an infrared lamp pearly source plate, the infrared light source 2 projects infrared light 4 towards the direction of the object to be measured, and the infrared light 4 reaches the object to be measured after being further homogenized by the diffusion plate 51. In one embodiment, the light diffusion box 6 is made of PC material and has an outer dimension of 50cm x 5cm, and the side facing the object 1 to be measured is frosted. The light diffusion box is low in cost, simple in manufacturing process and convenient to manufacture and apply. In one embodiment, a reflective projector may be mounted with a bracket at the edge of the light diffusion box 6.
In the behavior testing system, the infrared light source and the diffusion plate are concentrated in the light diffusion box, so that the size of the behavior testing system is reduced, and the system is convenient to assemble and easy to configure and use.
In one embodiment, the infrared camera may be composed of an infrared filter lens and a camera, wherein a light transmission band of the infrared filter lens is matched with a band of the infrared light source, so that the camera can only receive an infrared image of a band corresponding to the infrared light source, and the movement track of the object to be detected can be conveniently determined according to the shielding change of the object to be detected to the infrared light during the moving process. The camera can be an industrial grade high-speed camera, such as a high-speed black-and-white industrial camera based on an IEEE-1394 interface of a CCD (Charge-coupled Device) chip, the image acquisition speed is not lower than 25 frames/second, and the camera is connected with an infrared high-transmittance optical filter lens through a C/CS interface. The camera can shoot video images at a high frequency, so that a more accurate motion track of an object to be detected is ensured to be obtained.
In one embodiment, the behavioral testing system shown in fig. 1 may be used in the same direction as the image playing for infrared image acquisition. In order to acquire the motion track of the object to be detected in the direction perpendicular to the image playing direction, a camera can be configured in the direction perpendicular to the image playing direction, the image of the object to be detected in the direction perpendicular to the image playing direction is acquired, and the influence caused by the background image is small because no played image exists in the direction, so that the extraction of the motion track in the direction can be realized by adopting the image acquired by the common camera. The behavior testing system can acquire images in two mutually perpendicular directions to obtain the motion tracks of the object to be tested on two mutually perpendicular surfaces, and further can obtain the motion track of the object in a three-dimensional space, so that a more accurate motion track of the object to be tested is obtained, and the accuracy of behavior analysis is improved.
In one embodiment, the behavioral testing system shown in FIG. 1 may be employed for image acquisition in both directions perpendicular to each other. As shown in fig. 5, the first infrared light source 21 projects infrared light 4 toward the object 1 to be measured, the playing device 5 plays an image toward the object 1 to be measured, and the first infrared camera 31 collects an infrared image including the object 1 to be measured. The side surface of the object 1 to be measured, which is perpendicular to the projection direction of the infrared light 4 of the first infrared light source 21, is provided with a second infrared light source 22, which can project the infrared light source 4 to the object 1 to be measured. In one embodiment, the infrared light 4 reaching the object 1 to be measured is a uniform infrared light. The object 1 to be measured is located between the second infrared light source 22 and the second infrared camera 32, and the second infrared camera 32 can shoot an infrared image of the infrared light of the second infrared light source 22 after being shielded by the object to be measured.
The behavior testing system can collect the infrared images of two surfaces which are vertical to each other, so that the movement track of the object to be tested in a three-dimensional space can be obtained after the infrared images are processed, and the behavior testing system is favorable for improving the accuracy of behavior analysis.
In one embodiment, a first playing device may be disposed between the first infrared light source 21 and the object 1 to be measured, and plays an image toward the object 1 to be measured; a second playing device can also be arranged between the second infrared light source 22 and the object 1 to be measured, and an image is played towards the object 1 to be measured; or the playing devices are arranged between the first infrared light source 21 and the object 1 to be detected and between the second infrared light source 22 and the object 1 to be detected, so that a more real environment can be created, the motion track of the object to be detected in the space can be obtained, and the reaction of the object to be detected to the richer environment can be analyzed.
In one embodiment, as shown in FIG. 6. The short-focus projector 52 obtains the image to be played from the host 7, for example, the VGA input interface of the short-focus projector 52 is connected to the host 7 by using a VGA video connection line, and projects the image onto the diffusion plate 51 on the side of the light diffusion box 6. The first infrared light source 21 inside the light diffusion box 6 projects infrared light 4 in the direction of the object 1 to be measured, and the infrared light is projected on the object 1 to be measured after being homogenized by the diffusion plate 51. The first infrared camera 31 may be fixed by a bracket and takes an infrared image including the object 1 to be measured. In the direction perpendicular to the infrared image plane collected by the first infrared camera 31, the second infrared light source 22 projects infrared light 4 toward the object 1 to be measured, and the infrared light 4 reaches the object 1 to be measured after being homogenized by the diffusion plate 51 disposed above the second infrared light source 22. In one embodiment, a light diffusion box provided with a second infrared light source 22 may also be used to project infrared light 4 from this direction toward the object 1 to be measured. The infrared camera 32 may be fixed by a suspension bracket and takes an infrared image including the object 1 to be measured. The infrared images captured by the first infrared camera 31 and the second infrared camera 32 may be analyzed and processed by the image processing device 8, and the motion conditions of the object in the imaging plane of the infrared images are obtained. The image processing device 8 may be configured with a video capture card and video capture and behavior analysis software. Because the imaging planes of the two infrared images are perpendicular to each other, the image processing device 8 can obtain the motion condition of the object 1 to be measured in the three-dimensional space.
The behavior testing system can provide played images according to needs, the response of an object to be tested to the played images is obtained by utilizing infrared rays and infrared images, and the obtained motion trail is clearer as the influence of background images is eliminated; under the condition of obtaining the infrared images of two imaging planes which are perpendicular to each other, the motion trail of the object to be detected in the three-dimensional space can be determined, so that the behavior of the object to be detected can be analyzed more accurately and comprehensively. The image scene provided by the host computer to the playing device is changed, so that real and comprehensive research data between scene change and animal behaviors can be obtained.
A flow chart of one embodiment of a behavioral testing method of the present invention is shown in fig. 7.
In step 701, an infrared light source is used to project infrared light to an object to be measured from a side surface of the object to be measured. In one embodiment, the object to be tested may be an animal, such as zebrafish, located in a behavioral test box. In one embodiment, the uniform infrared light reaches the object to be measured, which is beneficial for the infrared camera to capture uniform infrared images.
In step 702, an infrared image of an area of the object to be measured is captured by an infrared camera on the opposite side of the object to be measured with respect to the infrared light source. In one embodiment, the behavior testing system shown in fig. 1 may be used, wherein the object to be tested is located between an infrared light source and an infrared camera, the infrared light source projects infrared light to the object to be tested, and the infrared camera shoots an infrared image towards the object to be tested, and the infrared image includes the object to be tested.
By the method, the infrared image of the object to be detected can be obtained by adopting the infrared light source irradiation and infrared image acquisition modes, so that the influence of the background image can be eliminated, the clear motion track of the object to be detected is obtained by utilizing the change of the infrared image caused by the shielding of the object to be detected on the infrared light, and the accuracy of behavior track acquisition of the object to be detected is improved.
A flow chart of another embodiment of the behavioral testing method of the present invention is shown in fig. 8.
In step 801, an image is played between the object to be measured and the infrared light source in the direction of the object to be measured by the playing device.
In step 802, an infrared light source is used to project infrared light to the object to be measured from the side of the object to be measured, and the infrared light emitted by the infrared light source can penetrate through the playing device and reach the object to be measured.
In step 803, an infrared image of the region of the object to be measured is taken by an infrared camera on the opposite side of the object to be measured from the infrared light source.
By such a method, the image can be played by the playing device, and the reaction of the object to be measured to the image can be observed. Because infrared light can see through play device, the mode that adopts infrared light source to shine and infrared light source to gather obtains the infrared image including the object that awaits measuring, can get rid of the image that plays static or change in the play device and cause the influence to the image that the camera was gathered to can confirm the movement track of the object that awaits measuring according to infrared image, provide the data basis for the analysis of the object that realizes awaiting measuring to the scene reaction.
In one embodiment, the playing device may be composed of a projector and a diffusion plate, the projector is located on a side surface of an area between the diffusion plate and the object to be measured, and projects an image toward a surface of the diffusion plate facing the object to be measured, so as to ensure that infrared light can pass through the diffusion plate to reach the object to be measured, and ensure that an infrared image collected by the infrared camera can reflect a motion trajectory of the object to be measured. The projector is arranged on the side face of the area between the diffusion plate and the object to be tested, so that infrared light is not shielded by the projector, strong light emitted by a bulb of the projector cannot interfere with the behavior of the object to be tested, and the accuracy of behavior test is improved.
In one embodiment, the projector is a short focus projector capable of projecting an image of a predetermined size on the diffuser plate over a short distance. In one embodiment, the short focus projector needs to ensure that the projected area is not less than 80cm x 80cm within a distance of 20 cm. By adopting the projector, the requirement on the distance between the diffusion plate and the object to be detected can be reduced, the size of the behavior detection system is reduced, the object to be detected can be ensured to see the played image in a short distance, and the influence of the image on the object to be detected is ensured.
In one embodiment, a reflective projector is used to project an image onto the diffuser plate. Because the short-focus projection of fisheye formula can produce image distortion, adopt reflective projector can provide better image and present the image quality, improve the degree of accuracy of behavioral testing.
In one embodiment, the infrared camera may be composed of an infrared filter lens and a camera, wherein a light transmission band of the infrared filter lens is matched with a band of the infrared light source, so that the camera can only receive an infrared image of a band corresponding to the infrared light source, and the movement track of the object to be detected can be conveniently determined according to the shielding change of the object to be detected to the infrared light during the moving process.
A flow chart of yet another embodiment of the behavioral testing method of the present invention is shown in fig. 9.
In step 901, two infrared light sources are used to project infrared light to the object to be measured from two mutually perpendicular side surfaces of the object to be measured, respectively.
In step 902, infrared images of the object area to be measured are respectively captured on opposite sides of the object to be measured with respect to the two infrared light sources.
By the method, the infrared images of the two surfaces which are perpendicular to each other and comprise the object to be detected can be collected, so that the more accurate motion track of the object to be detected is obtained, and the accuracy of behavior analysis is improved.
In one embodiment, the playing devices can be respectively arranged between the two infrared light sources and the object to be detected, so that a more real environment can be created, the motion track of the object to be detected in the space can be obtained, and the reaction of the object to be detected to the richer environment can be analyzed.
A flow chart of yet another embodiment of the behavioral testing method of the present invention is shown in fig. 10.
In step 1001, two infrared light sources are used to project infrared light to the object to be measured from two mutually perpendicular side surfaces of the object to be measured.
In step 1002, infrared images of the object area to be measured are respectively captured on opposite sides of the object to be measured with respect to the two infrared light sources.
In step 1003, image analysis and trajectory extraction of respective corresponding planes are performed according to two infrared images with mutually perpendicular imaging planes, and a motion trajectory of the object to be measured in a three-dimensional space is obtained according to the motion trajectories of the object to be measured in the two planes.
By the method, the motion trail of the object to be detected in the three-dimensional space can be determined under the condition of obtaining the infrared images of the two imaging planes which are perpendicular to each other, so that the behavior of the object to be detected can be analyzed more accurately and comprehensively.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention and not to limit it; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art will understand that: modifications to the specific embodiments of the invention or equivalent substitutions for parts of the technical features may be made; without departing from the spirit of the present invention, it is intended to cover all aspects of the invention as defined by the appended claims.
Claims (15)
1. A behavioral testing system, comprising:
an infrared light source;
the infrared camera comprises an infrared filter lens and a camera, and a light-transmitting waveband of the infrared filter lens is matched with the waveband of the infrared light source; and the combination of (a) and (b),
the playing device is positioned between the object to be detected and the infrared light source and comprises a projector and a diffusion plate; the diffusion plate is positioned between the object to be detected and the infrared light source; the projector is positioned on the side surface of the area between the diffusion plate and the object to be measured and is used for projecting images to the diffusion plate;
the infrared light source is used for projecting infrared light to the object to be detected;
the infrared camera is used for shooting an infrared image including the object to be detected;
the playing device is used for playing images to the direction of the object to be measured.
2. The system of claim 1,
the projector is a short-focus projector, is connected to the edge of the diffusion plate through a support and extends out towards the direction of the object to be measured.
3. The system of claim 2,
the short-focus projector is a reflective projector.
4. The system of claim 1,
the infrared light source comprises an infrared lamp bead light source plate and is positioned on the opposite side of the diffusion plate relative to the object to be detected.
5. The system of claim 4,
the diffusion board is the light diffusion case orientation the box panel of one side of the object of awaiting measuring, the light diffusion case is located the object side of awaiting measuring, infrared lamp pearl light source board is located the light diffusion incasement, and can see through the light diffusion case to the object of awaiting measuring throws infrared light.
6. The system of claim 1, wherein the infrared light source comprises a first infrared light source and a second infrared light source, and the infrared camera comprises a first infrared camera and a second infrared camera;
the object to be detected is positioned between the first infrared light source and the first infrared camera and between the second infrared light source and the second infrared camera;
the projection directions of the infrared light of the first infrared light source and the second infrared light source are mutually vertical.
7. The system of claim 6, further comprising:
the first playing device is positioned between the object to be tested and the first infrared light source and can play images towards the object to be tested; and/or the presence of a gas in the gas,
and the second playing device is positioned between the object to be tested and the second infrared light source and can play images towards the object to be tested.
8. The system of any one of claims 1 to 7, further comprising:
and the image processing device is connected with the infrared camera and used for determining the motion condition of the object to be detected on the imaging plane of the infrared image according to the infrared image.
9. The system of claim 1 or 7, further comprising:
and the host is connected with the playing device and provides the image.
10. The system of claim 1,
the wavelength of the infrared light is 930-950 nm; and/or the presence of a gas in the gas,
the image acquisition speed of the infrared camera is not lower than 25 frames/second.
11. A method of behavioral testing, comprising:
projecting infrared rays from the side surface of an object to be detected to the object to be detected by using an infrared light source;
between the object to be measured and the infrared light source, through play device to the object direction broadcast image that awaits measuring includes: projecting an image to a diffusion plate through a projector, wherein the playing device comprises the projector and the diffusion plate, the diffusion plate is located between the object to be measured and the infrared light source, and the projector is located on the side surface of an area between the diffusion plate and the object to be measured;
and shooting the infrared image of the area of the object to be detected through an infrared camera on the opposite side of the object to be detected relative to the infrared light source.
12. The method of claim 11,
the shooting of the infrared image of the object area to be detected through an infrared camera on the opposite side of the object to be detected relative to the infrared light source comprises:
filtering light rays by using an infrared filtering lens on the opposite side of the object to be measured relative to the infrared light source, wherein the light transmission waveband of the infrared filtering lens is matched with the waveband of the infrared light source;
and shooting the infrared image filtered by the infrared filter lens through a camera.
13. The method of claim 11,
the projecting of the infrared light from the side surface of the object to be measured to the object to be measured by using the infrared light source comprises the following steps:
projecting the infrared light to the object to be detected from two mutually vertical side surfaces of the object to be detected by utilizing the two infrared light sources respectively;
the shooting of the infrared image of the region of the object to be detected through an infrared camera on the opposite side of the object to be detected relative to the infrared light source comprises:
and respectively shooting the infrared images of the to-be-detected object area at the opposite sides of the to-be-detected object relative to the two infrared light sources.
14. The method of claim 13, further comprising:
and playing an image to the direction of the object to be detected through a playing device between the object to be detected and one or two infrared light sources.
15. The method of any of claims 11 to 14, further comprising:
and determining the motion condition of the object to be detected on the imaging plane of the infrared image according to the infrared image.
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