CN105994086A - Controllable zebra fish behavior testing system capable of simulating live action by projection - Google Patents
Controllable zebra fish behavior testing system capable of simulating live action by projection Download PDFInfo
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- 238000012360 testing method Methods 0.000 title claims abstract description 244
- 241000252212 Danio rerio Species 0.000 title abstract description 11
- 230000009471 action Effects 0.000 title abstract description 5
- 230000033001 locomotion Effects 0.000 claims abstract description 34
- 238000009792 diffusion process Methods 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 16
- 238000001914 filtration Methods 0.000 claims description 13
- 238000003384 imaging method Methods 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 6
- 238000011160 research Methods 0.000 abstract description 8
- 230000000144 pharmacologic effect Effects 0.000 abstract description 2
- 230000008859 change Effects 0.000 description 12
- 241001465754 Metazoa Species 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 6
- 238000000265 homogenisation Methods 0.000 description 6
- 239000011324 bead Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Marine Sciences & Fisheries (AREA)
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Abstract
The invention provides a controllable zebra fish behavior testing system capable of simulating live action by projection and relates to the field of medical and pharmacological basic researches. The behavior testing system provided by the invention comprises an infrared light source and an infrared camera, wherein an object to be detected can be arranged between the infrared light source and the infrared camera; the infrared light source is used for projecting infrared light to the object to be detected; and the infrared camera is used for photographing an infrared image of the object to be detected. The behavior testing system provided by the invention can obtain the infrared image comprising the object to be detected by adopting an infrared light source irradiation manner and an infrared light source collection manner, so that influences on a background image can be eliminated and a clear movement track of the object to be detected is obtained by utilizing changes of the infrared image, caused by the fact that the object to be detected shields the infrared light; and the accuracy of collecting behavior tracks of the object to be detected is improved.
Description
Technical field
The present invention relates to medical science and pharmacological basis research field, a kind of controlled projective module
Intend the Brachydanio rerio performance testing system of outdoor scene.
Background technology
Brachydanio rerio is a kind of Small Subtropical fresh-water fishes, as vertebrate model organism, its
Tract is the most similar to mammal with physiological function, and genome reaches with mankind's very high homology
85%, available Brachydanio rerio model as an alternative in pharmacology test, for new drug development and mechanism
Research provides significant data.Brachydanio rerio environmental change perception to external world is very sensitive, can produce and recognize
Knowing and the change of the aspect such as emotion, and then show the behavior reaction of complexity, this is at psychoneural row
For research field, there is the highest Research Significance, and how scientific research scene changes is anti-with behavior
Relation between Ying has bigger difficulty technically.
When using video acquisition and the change of analytical technology research environment affects for animal behavior,
Common practice is to utilize computer screen to play scenic picture in test box side, simultaneously to animal
Carrying out video record, the real-time or later stage carries out behavior analysis.This method use computer screen this
Plant and play entity so that be coaxially continually changing due to background to the video gathered, behavior analysis
Software cannot remove ambient interferences, thus can not realize accurately chasing after target animal movement track
Track.
Summary of the invention
It is an object of the present invention to propose a kind of improve what object under test action trail gathered
The scheme of accuracy.
According to an aspect of the present invention, a kind of performance testing system is proposed, including: infrared light
Source；And thermal camera, object under test can be positioned between infrared light supply and thermal camera；
Infrared light supply is for projecting Infrared to object under test；Thermal camera includes treating for shooting
Survey the infrared image of object.
Alternatively, also include: playing device, between object under test and infrared light supply, use
In playing image to object under test direction.
Alternatively, playing device includes scialyscope and diffusing panel；Diffusing panel be positioned at object under test with
Between infrared light supply；Scialyscope is the side in region between diffusing panel and object under test, is used for
To diffusing panel projects images.
Alternatively, scialyscope is short focus projector, is connected to the edge of diffusing panel also by support
Stretch out to object under test direction.
Alternatively, short focus projector is opaque projector.
Alternatively, infrared light supply includes infrared lamp pearly-lustre source plate, is positioned at diffusing panel relative to be measured
The opposition side of object.
Alternatively, diffusing panel is the cabinet panel of the light diffusion case side towards object under test, light
Diffusion case is positioned at object under test side, and infrared lamp pearly-lustre source plate is positioned at light diffusion case, and can
Infrared is projected to object under test through light diffusion case.
Alternatively, thermal camera includes infrared filtering camera lens and video camera, infrared filtering camera lens
Transmission region match with infrared light supply wave band.
Alternatively, infrared light supply includes the first infrared light supply and the second infrared light supply, infrared photography
Machine includes the first thermal camera and the second thermal camera；Object under test is positioned at the first infrared light
Between source and the first thermal camera, and be positioned at the second infrared light supply and the second thermal camera it
Between；The projecting direction of the Infrared of the first infrared light supply and the second infrared light supply is mutually perpendicular to.
Alternatively, also include: the first playing device, be positioned at object under test and the first infrared light supply
Between, it is possible to image is play to object under test direction.
Alternatively, also include: the second playing device, be positioned at object under test and the second infrared light supply
Between, it is possible to image is play to object under test direction.
Alternatively, also include: image processing apparatus, be connected with thermal camera, for root
The object under test motion conditions in image plane at infrared image is determined according to infrared image.
Alternatively, also include: main frame, be connected with playing device, it is provided that image.
Alternatively, the wavelength of Infrared is 930～950nm.
Alternatively, the image acquisition speed of thermal camera is not less than 25 frames/second.
Such performance testing system can use infrared light supply to irradiate and infrared image acquisition
Mode obtains the infrared image including object under test such that it is able to get rid of the impact of background image,
The change utilizing object under test that Infrared is blocked the infrared image caused is treated clearly
Survey movement locus of object, improve the accuracy that object under test movement locus gathers.
According to another aspect of the present invention, a kind of performance testing method is proposed, including: utilize
Infrared light supply projects Infrared from the side of object under test to object under test；In object under test phase
The infrared figure in thermal camera shooting object under test region is passed through in opposition side for infrared light supply
Picture.
Alternatively, also include: between object under test and infrared light supply, by playing device to
Image is play in object under test direction.
Alternatively, playing device includes scialyscope and diffusing panel；By playing device to determinand
Body direction is play image and is included: by scialyscope to diffusing panel projects images, wherein, diffusing panel
Between object under test and infrared light supply, scialyscope is district between diffusing panel and object under test
The side in territory.
Alternatively, clapped by thermal camera relative to the opposition side of infrared light supply at object under test
The infrared image taking the photograph object under test region includes: contrary relative to infrared light supply of object under test
Side, utilize infrared filtering camera lens filter light, wherein, the transmission region of infrared filtering camera lens with
The wave band of infrared light supply matches；Filtered through infrared filtering camera lens by video camera shooting
Infrared image.
Alternatively, infrared light supply is utilized to project infrared light from the side of object under test to object under test
Line includes: utilize two infrared light supplies from orthogonal two sides of object under test respectively to treating
Survey object projection Infrared；At object under test relative to the opposition side of infrared light supply by infrared
The infrared image in video camera shooting object under test region includes: red relative to two at object under test
The opposition side of outer light source shoots the infrared image in object under test region respectively.
Alternatively, also include: between object under test and one or two infrared light supply, pass through
Playing device plays image to object under test direction.
Alternatively, also include: determine the object under test imaging at infrared image according to infrared image
The motion conditions of plane.
Infrared light supply can be used to irradiate and by the way of infrared image acquisition by such method
Obtain the infrared image including object under test such that it is able to get rid of the impact of background image, utilize
Infrared is blocked the change of the infrared image caused and is obtained determinand clearly by object under test
Body movement locus, improves the accuracy that object under test movement locus gathers.
Accompanying drawing explanation
Accompanying drawing described herein is used for providing a further understanding of the present invention, constitutes the application
A part, the schematic description and description of the present invention is used for explaining the present invention, not structure
Become inappropriate limitation of the present invention.In the accompanying drawings:
Fig. 1 is the schematic diagram of an embodiment of the performance testing system of the present invention.
Fig. 2 is the schematic diagram of another embodiment of the performance testing system of the present invention.
Fig. 3 is the signal of an embodiment of the playing device of the performance testing system of the present invention
Figure.
Fig. 4 is the signal of an embodiment of the light diffusion case of the performance testing system of the present invention
Figure.
Fig. 5 is the schematic diagram of another embodiment of the performance testing system of the present invention.
Fig. 6 is the schematic diagram of the further embodiment of the performance testing system of the present invention.
Fig. 7 is the flow chart of an embodiment of the performance testing method of the present invention.
Fig. 8 is the flow chart of another embodiment of the performance testing method of the present invention.
Fig. 9 is the flow chart of another embodiment of the performance testing method of the present invention.
Figure 10 is the flow chart of the further embodiment of the performance testing method of the present invention.
Detailed description of the invention
Below by drawings and Examples, technical scheme is done and further retouches in detail
State.
The schematic diagram of one embodiment of the performance testing system of the present invention is as shown in Figure 1.Wherein,
2 is infrared light supply, and 3 is thermal camera, and object under test 1 is positioned at infrared light supply 2 and infrared takes the photograph
Between camera 3.Infrared light supply 2 projects Infrared 4 to object under test 1.Thermal camera 3
Shoot infrared image towards object under test 1 direction, infrared image includes object under test 1.Treat
Survey object 1 and can be in the animal in performance testing box, such as Brachydanio rerio etc..An enforcement
In example, arrive object under test for uniform Infrared, can be sent all by infrared light supply 2
Even Infrared 4, it is also possible to the Infrared sent by infrared light supply 2 is carried out at homogenization
Arrive object under test after reason, be so conducive to thermal camera to capture uniform infrared image.
Use such performance testing system, it is possible to use infrared light supply to irradiate and infrared image is adopted
The mode of collection obtains the infrared image including object under test such that it is able to get rid of the shadow of background image
Ringing, the change utilizing object under test that Infrared is blocked the infrared image caused obtains clearly
Object under test movement locus, improve object under test action trail gather accuracy.
When carrying out performance testing in an experiment at present, in order to avoid the impact of background image, can only
Take X to play scene, the mode of Y-direction video acquisition gather animal behavior (X to Y-direction
It is mutually perpendicular to).And aquatic animal this for Brachydanio rerio, lack the X analysis to travelling behavior
Data, will result in cannot objective evaluation scene changes for the impact of animal behavior, have a strong impact on
The verity of experimental result and reliability.Employing performance testing system as shown in Figure 1 can be real
Existing X plays with X to video acquisition to scene, will not lose scene and play the data in direction, also
The scene of broadcasting can be got rid of as the background image impact on trajectory extraction, improve animal row
For the accuracy analyzed, meet the needs of psychoneural praxiology research, be conducive in pharmacology
Learn and the application in neurobiological study field.
The schematic diagram of another embodiment of the performance testing system of the present invention is as shown in Figure 2.?
On the basis of performance testing system shown in Fig. 1, also include playing device 5.Playing device 5
Between infrared light supply 3 and object under test 1, Infrared 4 can pass through playing device 5
It is radiated on object under test 1.
Use such performance testing system, it is possible to utilizing playing device to play image, observation is treated
Survey the object reaction for image.Owing to Infrared can pass through playing device, use infrared
Light source irradiates and the mode of infrared light supply collection obtains the infrared image including object under test, it is possible to
Get rid of the image of the static or change play in playing device to camera acquisition to image cause
Impact such that it is able to determine the movement locus of object under test according to infrared image, to be measured for realizing
Object provides data basis for the analysis that scene is reacted.
In one embodiment, the structure of playing device 5 is as shown in Figure 3.Wherein, 51 is unrestrained
Penetrating plate, 52 is scialyscope.Scialyscope 52 is region between diffusing panel 51 and object under test 1
Side, to diffusing panel 51 towards the one side projects images of object under test 1.
Such performance testing system uses scialyscope to coordinate the mode of diffusing panel to play image, protects
Card Infrared can arrive object under test through diffusing panel, it is ensured that infrared camera scan red
Outer image can reflect the movement locus of object under test.Scialyscope is arranged on diffusing panel with to be measured
The side in region between object, while ensureing that scialyscope does not blocks Infrared, it is ensured that throw
The high light that shadow machine bulb sends does not affects the behavior of object under test, improves the standard of performance testing
Exactness.
In one embodiment, with support 53, scialyscope can be connected to the edge of diffusing panel,
Stretch out to object under test direction, thus avoid scialyscope to block the image that playing device is play, with
And avoid scialyscope to block Infrared.In one embodiment, support 53 is telescoping shoring column,
It is easy to flexible scialyscope and the position relationship of diffusing panel as required.
In one embodiment, scialyscope 52 is short focus projector, it is possible in shorter distance
The image of predefined size is projected on inherent diffusing panel 51.In one embodiment, short out-of-focus projection
Machine needs to ensure that in the distance of 20cm projected area is not less than 80cm*80cm.Use so
Scialyscope can reduce the requirement to diffusing panel Yu the spacing of object under test, reduce behavior inspection
While the volume of examining system, it is ensured that object under test in-plant can see the image of broadcasting,
Guarantee the image impact for object under test.
In one embodiment, use opaque projector to diffusing panel 51 projects images.By
Short out-of-focus projection chance in fish-eye type produces pattern distortion, uses opaque projector to provide
Better image imaging quality, improves the accuracy of performance testing.
In one embodiment, infrared light supply 2 can be infrared lamp pearly-lustre source plate, is positioned at broadcasting
Device 5, relative to the opposition side of object under test 1, projects Infrared to object under test 1 direction
4.Such infrared light supply light more disperses, uniformly, it is possible to make infrared image imaging uniform,
Be conducive to object under test trajectory analysis.Particularly after Infrared 4 penetrates diffusing panel 51, red
UV light 4, by further homogenization, further increases the imaging homogenization degree of infrared image.
In one embodiment, the size of infrared lamp pearly-lustre source plate can be about 48cm*48cm, by
The 100 infrared lamp bead of LEDs are spaced substantially equidistant composition, and every 10 lamp bead are connected into one group, group
In parallel with between group.Such size is conducive to the behavior of the object under test for Brachydanio rerio one class
Test.
In one embodiment, the emission wavelength of infrared light supply 2 is 930nm～950nm, excellent
Select 940nm.Meanwhile, thermal camera has the high filter lens thoroughly of 940nm narrow band ir.By
The red sudden and violent phenomenon of infrared light pearl in wavelength 940nm is more weak, it is possible to obtain apparent infrared figure
Picture, reaches preferably to test effect.
In one embodiment, as shown in Figure 4,6 is light diffusion case, and object under test 1 is positioned at
Between light diffusion case 6 and thermal camera 3.Light diffusion case 6 is towards the one side of object under test 1
For diffusing panel 51.It can be infrared that there are infrared light supply 2, infrared light supply 2 in light diffusion case 6 inside
Lamp bead light source board, infrared light supply 2 projects Infrared 4, Infrared 4 to object under test direction
Object under test is arrived after the further homogenization of diffusing panel 51.In one embodiment, light diffusion
Case 6 is PC material, and outside dimension is 50cm*50cm*5cm, towards the one side of object under test 1
Do frosted to process.Such light diffusion case is relatively low at cost, and processing technology is relatively simple, it is simple to
Make application.In one embodiment, support can be utilized to fix at the edge of light diffusion case 6
Opaque projector.
In such performance testing system, infrared light supply, diffusing panel are concentrated in light diffusion case,
On the one hand be conducive to reducing the volume of performance testing system, on the other hand make system assembles convenient,
It is prone to configuration use.
In one embodiment, thermal camera can be by infrared filtering camera lens and image mechanism
Becoming, wherein, the transmission region of infrared filtering camera lens matches with the wave band of infrared light supply, thus
Make video camera can only receive the infrared image of infrared light supply correspondence wave band, convenient according to be measured
Infrared is blocked change by object moving process and determines the movement locus of object under test.Take the photograph
Camera can be technical grade high-speed camera, as based on CCD (Charge-coupled Device,
Charge coupled cell) the high speed black and white industrial camera of IEEE-1394 interface of chip, image is adopted
Collection speed is not less than 25 frames/second, connects infrared height by C/CS interface and thoroughly filters camera lens.Such
Video camera can with higher frequency shooting video image, thus ensure to obtain treating more accurately
Survey the movement locus of object.
In one embodiment, can be in the behavior used in the same direction as shown in Figure 1 of image player
Test system carries out the collection of infrared image.It is being perpendicular to image player to gather object under test
The movement locus in direction, video camera can be configured in the direction that be perpendicular to image player, gather
The image of the object under test being perpendicular on the direction of image player, broadcasts owing to not existing in the direction
The image put, the impact that background image causes is little, therefore uses the image that common camera gathers
I.e. it is capable of the extraction of movement locus in the direction.Such performance testing system can be in phase
Gather image in the most vertical both direction, obtain object under test in orthogonal two faces
Movement locus, and then object can be obtained at three-dimensional movement locus, thus obtain more
Object under test movement locus accurately, is conducive to improving the accuracy of behavior analysis.
In one embodiment, all can use as shown in Figure 1 in orthogonal both direction
Performance testing system carry out image acquisition.As it is shown in figure 5, the first infrared light supply 21 is to be measured
The direction projection Infrared 4 of object 1, playing device 5 is play to the direction of object under test 1
Image, the first thermal camera 31 gathers the infrared image including object under test 1.At determinand
It is red that the side of Infrared 4 projecting direction that body 1 is perpendicular to the first infrared light supply 21 has second
Outer light source 22, it is possible to project infrared light supply 4 to object under test 1.In one embodiment, arrive
The Infrared 4 reaching object under test 1 is uniform Infrared.It is red that object under test 1 is positioned at second
Between outer light source 22 and the second thermal camera 32, the second thermal camera 32 can shoot
The Infrared of two infrared light supplies 22 infrared image after object under test blocks.
Such performance testing system can gather the infrared image in orthogonal two faces, from
And obtain object under test after being easy to infrared image is processed at three-dimensional movement locus,
Be conducive to improving the accuracy of behavior analysis.
In one embodiment, can join between the first infrared light supply 21 and object under test 1
Put the first playing device, play image to object under test 1 direction；Can also be at the second infrared light
Configure the second playing device between source 22 and object under test 1, play figure to object under test 1 direction
Picture；Or between the first infrared light supply 21 and object under test 1, and at the second infrared light supply
Playing device is all configured such that it is able to build more real environment between 22 and object under test 1,
Obtain the object under test movement locus in space, analyze object under test to more abundant environment
Reaction.
In one embodiment, as shown in Figure 6.Short focus projector 52 obtains from main frame 7 and needs
Image to be play, as utilized the VGA input interface of short focus projector 52 to use VGA to regard
Frequently connecting line is connected with main frame 7, and is projected on the diffusing panel 51 of light diffusion case 6 side.
The first infrared light supply 21 within light diffusion case 6 projects Infrared to the direction of object under test 1
4, it is incident upon on object under test 1 after diffusing panel 51 homogenization.First thermal camera 31
Can be fixed by support, shooting includes the infrared image of object under test 1.Infrared with first
On the vertical direction of infrared image plane that video camera 31 gathers, the second infrared light supply 22 is to treating
Surveying object 1 and project Infrared 4, Infrared 4 is configured above the second infrared light supply 22
Diffusing panel 51 homogenization after arrive object under test 1.In one embodiment, it would however also be possible to employ
The light diffusion case being provided with the second infrared light supply 22 projects infrared to object under test 1 from the direction
Light 4.Thermal camera 32 can be fixed by suspension holdfast, and shooting includes object under test 1
Infrared image.The infrared figure of first thermal camera the 31, second thermal camera 32 shooting
As being analyzed processing by image processing apparatus 8, respectively obtain object at infrared image
The motion conditions in image plane.Image processing apparatus 8 can be configured with video frequency collection card,
Video acquisition and behavior analysis software.Due to being mutually perpendicular in image plane of two infrared images, because of
This, image processing apparatus 8 can obtain object under test 1 in three-dimensional motion conditions.
Such performance testing system can provide the image of broadcasting as required, utilizes infrared light
Line and infrared image obtain the reaction of the object under test image for playing, owing to eliminating background
The impact of image, the movement locus obtained becomes apparent from；Obtaining orthogonal two imagings
In the case of the infrared image of plane, it is possible to determine object under test at three-dimensional movement locus,
Thus realize measuring targets behavior more accurately, comprehensively analyze.Carried by conversion main frame
The image scene of supply playing device, it is possible to obtain true comprehensively scene change and animal behavior
Between data.
The flow chart of one embodiment of the performance testing method of the present invention is as shown in Figure 7.
In step 701, infrared light supply is utilized to project from the side of object under test to object under test
Infrared.In one embodiment, moving during object under test can be in performance testing box
Thing, such as Brachydanio rerio etc..In one embodiment, arrive object under test for uniform infrared light
Line, is so conducive to thermal camera to capture uniform infrared image.
In a step 702, taken the photograph by infrared relative to the opposition side of infrared light supply at object under test
The infrared image in camera shooting object under test region.In one embodiment, can use such as figure
Performance testing system shown in 1, object under test between infrared light supply and thermal camera,
Infrared light supply projects Infrared to object under test, and thermal camera is clapped towards object under test direction
Taking the photograph infrared image, infrared image includes object under test.
By such method, it is possible to use infrared light supply to irradiate and the mode of infrared image acquisition
Obtain the infrared image including object under test such that it is able to get rid of the impact of background image, utilize
Infrared is blocked the change of the infrared image caused and is obtained determinand clearly by object under test
Body movement locus, improves the accuracy that object under test action trail gathers.
The flow chart of another embodiment of the performance testing method of the present invention is as shown in Figure 8.
In step 801, between object under test and infrared light supply, by playing device to treating
Survey object direction and play image.
In step 802, infrared light supply is utilized to project from the side of object under test to object under test
Infrared, the Infrared that infrared light supply sends can penetrate playing device and arrive determinand
Body.
In step 803, taken the photograph by infrared relative to the opposition side of infrared light supply at object under test
The infrared image in camera shooting object under test region.
By such method, it is possible to utilize playing device to play image, observe object under test pair
Reaction in image.Owing to Infrared can pass through playing device, infrared light supply is used to irradiate
The infrared image including object under test is obtained, it is possible to get rid of and play with the mode of infrared light supply collection
In device play static or change image to camera acquisition to image impact, from
And the movement locus of object under test can be determined according to infrared image, for realize object under test for
The analysis of scene reaction provides data basis.
In one embodiment, playing device can be made up of scialyscope and diffusing panel, scialyscope
The side in region between diffusing panel and object under test, to diffusing panel towards the one of object under test
Face projects images, thus ensure that Infrared can arrive object under test through diffusing panel, it is ensured that
The infrared image of infrared camera scan can reflect the movement locus of object under test.By scialyscope
It is arranged on the side in region between diffusing panel and object under test, is ensureing that scialyscope does not block infrared
While light, it is ensured that the high light that projecting camera light bulb sends does not affects the behavior of object under test,
Improve the accuracy of performance testing.
In one embodiment, scialyscope is short focus projector, it is possible in shorter distance
The image of predefined size is projected on diffusing panel.In one embodiment, short focus projector needs
In the distance of 20cm, ensure that projected area is not less than 80cm*80cm.Use such projection
Machine can reduce the requirement to diffusing panel Yu the spacing of object under test, reduces behavioral value system
Volume while, it is ensured that object under test in-plant can see the image of broadcasting, it is ensured that figure
As the impact for object under test.
In one embodiment, use opaque projector to diffusing panel projects images.Due to fish
The short out-of-focus projection chance of eye produces pattern distortion, uses opaque projector can provide more preferable
Image imaging quality, improve performance testing accuracy.
In one embodiment, thermal camera can be by infrared filtering camera lens and image mechanism
Becoming, wherein, the transmission region of infrared filtering camera lens matches with the wave band of infrared light supply, thus
Make video camera can only receive the infrared image of infrared light supply correspondence wave band, convenient according to be measured
Infrared is blocked change by object moving process and determines the movement locus of object under test.
The flow chart of another embodiment of the performance testing method of the present invention is as shown in Figure 9.
In step 901, utilize two infrared light supplies from orthogonal two sides of object under test
Face projects Infrared to object under test respectively.
In step 902, clap respectively relative to the opposition side of two infrared light supplies at object under test
Take the photograph the infrared image in object under test region.
By such method, it is possible to gather the object under test that includes in orthogonal two faces
Infrared image, thus obtain object under test movement locus the most accurately, be conducive to improving behavior
The accuracy analyzed.
In one embodiment, can be respectively configured between infrared light supply and object under test at two
Playing device such that it is able to build more real environment, it is thus achieved that object under test is in the fortune in space
Dynamic track, analyzes the object under test reaction to more abundant environment.
The flow chart of the further embodiment of the performance testing method of the present invention is as shown in Figure 10.
In step 1001, utilize two infrared light supplies from object under test orthogonal two
Side projects Infrared to object under test respectively.
In step 1002, distinguish relative to the opposition side of two infrared light supplies at object under test
The infrared image in shooting object under test region.
In step 1003, carry out each according to two infrared images orthogonal in image plane
The graphical analysis of corresponding flat and trajectory extraction, and according to object under test in the motion of two planes
Track obtains object under test at three-dimensional movement locus.
By such method, obtaining orthogonal two infrared images in image plane
In the case of, it is possible to determine object under test at three-dimensional movement locus, thus realize to be measured
Object behavior more accurately, comprehensively analyze.
Finally should be noted that: above example only in order to technical scheme to be described and
Non-to its restriction；Although the present invention being described in detail with reference to preferred embodiment, affiliated
The those of ordinary skill in field is it is understood that still can enter the detailed description of the invention of the present invention
Row is revised or portion of techniques feature is carried out equivalent；Without deviating from technical solution of the present invention
Spirit, it all should be contained in the middle of the technical scheme scope that the present invention is claimed.
Claims (20)
1. a performance testing system, it is characterised in that including:
Infrared light supply；With,
Thermal camera, object under test can be positioned over described infrared light supply and described infrared photography
Between machine；
Described infrared light supply is for projecting Infrared to described object under test；
Described thermal camera includes the infrared image of described object under test for shooting.
System the most according to claim 1, it is characterised in that also include:
Playing device, between described object under test and described infrared light supply, for described
Image is play in object under test direction.
System the most according to claim 2, it is characterised in that
Described playing device includes scialyscope and diffusing panel；
Described diffusing panel is between described object under test and described infrared light supply；
Described scialyscope is the side in region between described diffusing panel and described object under test, uses
In to described diffusing panel projects images.
System the most according to claim 3, it is characterised in that
Described scialyscope is short focus projector, is connected to the edge of described diffusing panel also by support
Stretch out to object under test direction.
System the most according to claim 4, it is characterised in that
Described short focus projector is opaque projector.
System the most according to claim 3, it is characterised in that
Described infrared light supply includes infrared lamp pearly-lustre source plate, is positioned at described diffusing panel relative to described
The opposition side of object under test.
System the most according to claim 6, it is characterised in that
Described diffusing panel is the light diffusion case cabinet panel towards the side of described object under test, institute
Stating light diffusion case and be positioned at described object under test side, described infrared lamp pearly-lustre source plate is positioned at described light
In diffusion case, and described infrared light can be projected through described light diffusion case to described object under test
Line.
System the most according to claim 1 and 2, it is characterised in that:
Described thermal camera includes infrared filtering camera lens and video camera, described infrared filtering camera lens
Transmission region match with described infrared light supply wave band.
System the most according to claim 1, it is characterised in that described infrared light supply bag
Including the first infrared light supply and the second infrared light supply, described thermal camera includes the first infrared photography
Machine and the second thermal camera；
Described object under test be positioned at described first infrared light supply and described first thermal camera it
Between, and between described second infrared light supply and described second thermal camera；
The projection of the described Infrared of described first infrared light supply and described second infrared light supply
Direction is mutually perpendicular to.
System the most according to claim 9, it is characterised in that also include:
First playing device, between described object under test and described first infrared light supply, energy
Enough to described object under test direction broadcasting image；And/or,
Second playing device, between described object under test and described second infrared light supply, energy
Enough to described object under test direction broadcasting image.
11. according to the arbitrary described system of claim 1～10, it is characterised in that also include:
Image processing apparatus, is connected with described thermal camera, for according to described infrared figure
As determining the described object under test motion conditions in image plane at described infrared image.
12. according to the system described in claim 2 or 10, it is characterised in that also include:
Main frame, is connected with described playing device, it is provided that described image.
13. systems according to claim 1, it is characterised in that
The wavelength of described Infrared is 930～950nm；And/or,
The image acquisition speed of described thermal camera is not less than 25 frames/second.
14. 1 kinds of performance testing methods, it is characterised in that including:
Infrared light supply is utilized to project Infrared from the side of object under test to described object under test；
Thermal camera is passed through relative to the opposition side of described infrared light supply at described object under test
Shoot the infrared image in described object under test region.
15. methods according to claim 14, it is characterised in that also include:
Between described object under test and described infrared light supply, by playing device to described to be measured
Image is play in object direction.
16. methods according to claim 15, it is characterised in that described playing device
Including scialyscope and diffusing panel；
Described image of being play to described object under test direction by playing device is included:
By described scialyscope to described diffusing panel projects images, wherein, described diffusing panel is positioned at
Between described object under test and described infrared light supply, described scialyscope is positioned at described diffusing panel and institute
State the side in region between object under test.
17. methods according to claim 14, it is characterised in that
Thermal camera is passed through relative to the opposition side of described infrared light supply at described object under test
The infrared image shooting described object under test region includes:
At described object under test relative to the opposition side of described infrared light supply, utilize infrared filter
Head filters light, wherein, the transmission region of described infrared filtering camera lens and described infrared light supply
Wave band matches；
By video camera shooting through the filtered described infrared image of described infrared filtering camera lens.
18. methods according to claim 14, it is characterised in that
Described utilize infrared light supply from the side of object under test to described object under test projection infrared
Light includes:
Two described infrared light supplies are utilized to divide from orthogonal two sides of described object under test
Do not project described Infrared to described object under test；
Described taken the photograph by infrared relative to the opposition side of described infrared light supply at described object under test
Camera shoots the infrared image in described object under test region and includes:
Institute is shot respectively relative to the opposition side of two described infrared light supplies at described object under test
State the infrared image in object under test region.
19. methods according to claim 18, it is characterised in that also include:
Between described object under test and one or two described infrared light supply, pass through playing device
Image is play to described object under test direction.
20. according to the arbitrary described method of claim 14～19, it is characterised in that also include:
Determine that described object under test is put down at the imaging of described infrared image according to described infrared image
The motion conditions in face.
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| CN201610537092.6A CN105994086B (en) | 2016-07-08 | 2016-07-08 | Zebra fish behavior test system capable of simulating real scene through controllable projection |
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| CN106889006A (en) * | 2016-12-30 | 2017-06-27 | 浙江海洋大学 | A kind of Multifunctional fish types of populations behavior pond |
| CN110574718A (en) * | 2019-09-18 | 2019-12-17 | 华中农业大学 | Fish reproduction spawning behavior research system |
| CN114304005A (en) * | 2021-12-28 | 2022-04-12 | 中国科学院生物物理研究所 | Accurate visual stimulation's toy action recorder |
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| CN206165502U (en) * | 2016-07-08 | 2017-05-17 | 中国人民解放军军事医学科学院毒物药物研究所 | Performance testing system |
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| US5650135A (en) * | 1994-07-01 | 1997-07-22 | The Board Of Trustees Of The Leland Stanford Junior University | Non-invasive localization of a light-emitting conjugate in a mammal |
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| CN106889006A (en) * | 2016-12-30 | 2017-06-27 | 浙江海洋大学 | A kind of Multifunctional fish types of populations behavior pond |
| CN106889006B (en) * | 2016-12-30 | 2020-11-10 | 浙江海洋大学 | Multifunctional fish group behavior pool |
| CN110574718A (en) * | 2019-09-18 | 2019-12-17 | 华中农业大学 | Fish reproduction spawning behavior research system |
| CN110574718B (en) * | 2019-09-18 | 2021-04-09 | 华中农业大学 | Fish reproduction spawning behavior research system |
| CN114304005A (en) * | 2021-12-28 | 2022-04-12 | 中国科学院生物物理研究所 | Accurate visual stimulation's toy action recorder |
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| CN105994086B (en) | 2022-02-11 |
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