CN105987907A - Detection kit for on-site quick detection of iron ions in water and preparing method - Google Patents

Detection kit for on-site quick detection of iron ions in water and preparing method Download PDF

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Publication number
CN105987907A
CN105987907A CN201510095223.5A CN201510095223A CN105987907A CN 105987907 A CN105987907 A CN 105987907A CN 201510095223 A CN201510095223 A CN 201510095223A CN 105987907 A CN105987907 A CN 105987907A
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China
Prior art keywords
iron ion
water
liquid
detection
color
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Pending
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CN201510095223.5A
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Chinese (zh)
Inventor
苏波波
吴秀萍
毛丽灿
连国军
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Wenzhou Medical University
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Wenzhou Medical University
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Priority to CN201510095223.5A priority Critical patent/CN105987907A/en
Publication of CN105987907A publication Critical patent/CN105987907A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a detection kit which is used for on-site quick detection of iron ions in water and capable of eliminating reagent blank influences and a preparing method of the kit. In a faintly acid medium, iron in water reacts with 2-(5-bromo-2-pyridylazo)-5-(diethylamino)phenol to generate a purple red complex, and the shade of the purple red complex is in direct proportion to the concentration of iron ions; a comparison with a test tube is made after the color of an iron ion standard color chart with reagent blank eliminated and a blank tube color are overlaid, and the concentration of iron ions in a water sample to be tested is obtained. The detection kit is especially suitable for on-site quick detection of iron ions in water.

Description

Iron ion field quick detection kit and preparation method in water
Technical field
The present invention relates to field of water quality detection, particularly relate to the on-the-spot quick inspection of iron ion in a kind of water Test agent box and preparation method.
Background technology
Iron (Fe) is the trace element of needed by human, and internal people has many-sided physiological function, Human body occupies corresponding proportion by the trace element absorbing of drinking water in daily life, and iron ion contains Amount detection is country's drinking water standard regulation detection project
The detection of iron ion in water uses atomic absorption spectrophotometry, visible light light-splitting luminosity more Method, inductively coupled plasma atomic emission spectrum/mass spectrography, electrochemical methods etc., this A little methods are both needed to take back water sample special laboratory, have been chemically examined by professional operating technology personnel Becoming, major part method also needs to the high large-scale instrumentation of price, it is impossible to meet quick, simple Just Site Detection requires, some special when and wheres there is also sampling not in time, can not Objectively respond the situation of sampling location Water quality.
Existing in the market iron ion Site Detection reagent mostly is based on colorimetric method Dry-type detects reagent, and this detection reagent takes a certain amount of water sample and dry-type when measuring Detection reagent mixes, dissolves, produces specific color after reaction a period of time, direct and standard Colour atla comparison draws the concentration of water sample test substance.This type of method is quick, convenient, public participation Degree height, but the impact on measurement result for the change of reagent blank during measurement, cannot be eliminated, only exist Just there is when reagent blank is invariable higher accuracy.But, actually detected discovery, nothing Opinion is the detection reagent of liquid-type or dry-type, all inevitably exists in storing process The situation that reagent blank becomes larger or diminishes.Additionally, the detection reagent of use dry-type is carried out During water sample detection, need first with water sample to be measured detection reagent fully dissolve, then with in water sample Concrete pollutant carries out reaction and produces particular color.Owing to Various Seasonal, zones of different gather TWS is not quite similar, and when dissolving dry-type detection reagent, required time is also not quite similar, whole Individual detection process required time is difficult to effectively control, and it is unfavorable also to cause the accuracy of testing result Impact.
Therefore, demand is a kind of uses liquid-type to detect reagent, can eliminate reagent blank to detection knot The detection reagent of iron ion in the quantitative water of fruit impact, the use direct visual colorimetric determination of standard color card Box, for realizing that in water, iron ion field quick detection is particularly important.
Content of the invention
The technical problem to be solved is to provide for one can eliminate reagent blank impact Water in iron ion field quick detection box, to this end, the present invention also provides this kit a kind of Preparation method.
The principle of kit of the present invention is: in weak acidic medium, the iron in water and 2-(the bromo-2-of 5- Pyridylazo)-5-diethylamino phenol reaction generation aubergine complex, this aubergine complex Shade is directly proportional to iron concentration, by the iron ion standard color card face of deduction reagent blank Contrast with testing tube after look and blank tube color addition, draw iron concentration in water sample to be measured.
For solving above-mentioned technical problem, the technical solution adopted in the present invention is:
For the kit of iron ion field quick detection in water, consisting of of it:
1st, liquid-type iron ion detection reagent: 60mmol/L pH3.5 Acetic acid-sodium acetate buffer solution, Tell containing 0.15-0.25g/L 2-(5-Bromo-2-pyridylazo)-5-diethylamino phenol, 0.1-0.5ml/L Temperature-80,2-10g/L anhydrous sodium sulfite, 50-100g/L vitamin C, 10-20g/L thiocarbamide, 30-70ml/L ethylene glycol.
2nd, iron ion standard color card: by deduction reagent blank, concentration be respectively 0.06mg/L, The iron ion standard of 0.12mg/L, 0.18mg/L, 0.24mg/L, 0.30mg/L, 0.36mg/L Reference colour composition produced by solution reaction.
The preparation method of aforesaid liquid sections ion detection reagent is: weigh 0.15-0.25g 2-(5- Bromo-2-is than pyridine azo)-5-diethylamino phenol, 2-10g anhydrous sodium sulfite, 50-100g tie up raw Element C, 10-20g thiocarbamide, adds 800ml 60mmol/L pH3.5 Acetic acid-sodium acetate buffer solution and stirs Mix dissolving, add 0.1-0.5ml Tween-80,30-70ml ethylene glycol, use after stirring and dissolving 60mmol/L pH3.5 Acetic acid-sodium acetate buffer solution is settled to 1000ml.
The preparation method of above-mentioned iron ion standard color card is: precision weighs 0.1000g content and is The metallic iron of 99.99%, is settled to 1000ml after dissolving with the nitric acid of 10ml 50%, obtains dense Iron ion storing solution is being diluted by the iron ion storing solution that degree is 100mg/L by deionized water Become concentration be 0.06mg/L, 0.12mg/L, 0.18mg/L, 0.24mg/L, 0.30mg/L, The iron ion standard liquid of 0.36mg/L;Take 7 10ml glass colorimetric cylinders, arrange the 1st Colorimetric cylinder be blank tube, 2-7 root colorimetric cylinder be standard pipe, respectively at blank tube and standard pipe Middle addition 10ml deionized water and 10ml iron ion standard liquid, add 0.2ml liquid-type Iron ion detects reagent, 5-40 DEG C of reaction 5-15 minute;Clapped by colorimetric cylinder bottom up and take each pipe Each standard pipe color is deducted by the produced color of reaction by photoshop 7.0 mapping software After blank tube color, the color of gained and the concentration of corresponding iron ion standard liquid are printed upon Thickness is on the water white transparency PVC plastic flitch of 1-2mm, obtains the iron after deducting reagent blank Ion standard color card.
The positive effect of the present invention is as follows:
There is the advantage that method is simple, accuracy is high, easily operated;Use liquid-type iron ion Detection reagent detects, and can effectively control whole detection process required time;By by blank The method that pipe color addition carries out colorimetric control with sample cell on standard color card again, can effectively disappear It except the impact on testing result for the reagent blank, is particularly suitable for iron ion field quick detection in water.
The detection kit of the present invention and the existing iron ion using standard color card direct color comparison Detection kit compares, and the results are shown in Table 1.
Table 1
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further, the following stated, is only to this The preferred embodiment of invention, not does the restriction of other forms to the present invention, any familiar Professional and technical personnel is changed to become on an equal basis possibly also with the technology contents of the disclosure above The Equivalent embodiments changed.Every without departing from the present invention program content, the technology according to the present invention is real Any simple modification that confrontation following example are done or equivalent variations, all fall within the guarantor of the present invention In the range of protecting.
Embodiment 1
Iron ion field quick detection kit in a kind of water, this kit is by following material group Become:
Liquid-type iron ion detection reagent 1 bottle (10ml)
Iron ion standard color card 1
The preparation method of this kit is through the following steps that realize:
1st, the preparation method of liquid-type iron ion detection reagent:
(1) 0.20g 2-(5-Bromo-2-pyridylazo)-5-diethylamino phenol, 6g anhydrous sodium, are weighed Sodium sulphate, 75g vitamin C, 15g thiocarbamide, add 800ml 60mmol/L pH3.5 acetic acid- Sodium-acetate buffer stirring and dissolving;
(2), 0.3ml Tween-80,50ml ethylene glycol, stirring and dissolving are added;
(3) it is settled to 1000ml with 60mmol/L pH3.5 Acetic acid-sodium acetate buffer solution.
2nd, the preparation method of iron ion standard color card:
(1), precision weighs the metallic iron that 0.1000g content is 99.99%, with 10ml 50% Nitric acid dissolve after be settled to 1000ml, obtain the iron ion storing solution that concentration is 100mg/L, With deionized water iron ion storing solution is diluted to concentration be 0.06mg/L, 0.12mg/L, The iron ion standard liquid of 0.18mg/L, 0.24mg/L, 0.30mg/L, 0.36mg/L;
(2), take 7 10ml glass colorimetric cylinders, arrange the 1st colorimetric cylinder be blank tube, 2-7 root colorimetric cylinder is standard pipe, respectively in blank tube and standard pipe add 10ml go from Sub-water and 10ml iron ion standard liquid, add 0.2ml liquid-type iron ion detection reagent, React 10 minutes at 20 DEG C;
(3), the bat of colorimetric cylinder bottom up is taken color produced by each tube reaction, use After each standard pipe color is deducted blank tube color by photoshop 7.0 mapping software, by gained The concentration of color and corresponding iron ion standard liquid is printed upon the water white transparency that thickness is 1mm On PVC plastic flitch, obtain the iron ion standard color card after deducting reagent blank.
Detection method:
Take 2 10ml glass colorimetric cylinders, arrange the 1st colorimetric cylinder be blank tube, the 2nd Colorimetric cylinder is sample cell, respectively in blank tube and sample cell add 10ml deionized water and 10ml water sample to be measured, adds liquid-type iron ion detection reagent 0.2ml, 20 DEG C of reactions 10 minutes;Standard color card is placed in bottom blank tube, colorimetric cylinder bottom up is observed blank Pipe superpose with standard color card after color, and with sample cell color comparator, when both solid colours When, in 10 minutes, read the iron concentration corresponding to standard color card, draw iron in water sample Ion concentration.
Embodiment 2
In order to prove the creativeness of the present invention further, the present invention has done following contrast test, examines That examines reagent blank changes the impact on measurement result accuracy.As described in this law embodiment 1 In water, iron ion field quick detection kit is test group, not deduct reagent blank, employing The detection kit of standard color card direct color comparison is control group, by the detection examination in two group reagent boxes 4 DEG C of refrigerators are put in agent respectively and 37 water-baths store 7 days, and to same portion Atomic absorption Method measures the water sample that iron concentration is 0.06mg/L and is measured, and uses deionized water generation simultaneously Carry out reagent blank test for water sample, with 721 spectrophotometer record reagent blank values, test When cuvette optical path be 1cm, test wavelength is 560nm, and reference solution is deionized water, knot Fruit is shown in Table 2.
Table 2
From table 2 it can be seen that under identical experiment condition, with the gradually increasing of reagent blank Greatly, the measurement result of test group keeps constant, but the measurement result of control group substantially becomes big, table Bright method of the present invention can effectively remove the change of reagent blank to iron ion test result Impact.
Although an embodiment of the present invention has been shown and described, the ordinary skill for this area For personnel, it is possible to understand that without departing from the principles and spirit of the present invention can be to this A little embodiments carry out multiple change, modification, replacement and modification, and the scope of the present invention is by appended power Profit requires and equivalent limits.

Claims (3)

1. the kit for iron ion field quick detection in water, it is characterised in that: its composition For:
(1), liquid-type iron ion detection reagent: 60mmol/L pH3.5 Acetic acid-sodium acetate delays Rush liquid, containing 0.15-0.25g/L 2-(5-bromo-2-pyridine azo)-5-diethylamino phenol, 0.1-0.5ml / L Tween-80,2-10g/L anhydrous sodium sulfite, 50-100g/L vitamin C, 10-20g/L Thiocarbamide, 30-70ml/L ethylene glycol;
(2), iron ion standard color card: by deduction reagent blank, concentration be respectively 0.06mg/L, The iron ion standard of 0.12mg/L, 0.18mg/L, 0.24mg/L, 0.30mg/L, 0.36mg/L Reference colour composition produced by solution reaction.
2. the kit for iron ion field quick detection in water according to claim 1 Preparation method, it is characterised in that: the preparation method of liquid-type iron ion detection reagent is: weigh 0.15-0.25g 2-(5-Bromo-2-pyridylazo)-5-diethylamino phenol, 2-10g anhydrous sodium sulfite, 50-100g vitamin C, 10-20g thiocarbamide, add 800ml 60mmol/L pH3.5 acetic acid-vinegar Acid sodium buffer solution stirring and dissolving, adds 0.1-0.5ml Tween-80,30-70ml ethylene glycol, It is settled to 1000ml with 60mmol/L pH3.5 Acetic acid-sodium acetate buffer solution after stirring and dissolving.
3. the kit for iron ion field quick detection in water according to claim 1 Preparation method, it is characterised in that: the preparation method of iron ion standard color card is: precision weighs 0.1000g content is the metallic iron of 99.99%, is settled to after dissolving with the nitric acid of 10ml 50% 1000ml, obtains the iron ion storing solution that concentration is 100mg/L, by deionized water by iron Ion stock liquid be diluted to concentration be 0.06mg/L, 0.12mg/L, 0.18mg/L, 0.24mg/L, The iron ion standard liquid of 0.30mg/L, 0.36mg/L;Take 7 10ml glass colorimetric cylinders, Arrange the 1st colorimetric cylinder be blank tube, 2-7 root colorimetric cylinder be standard pipe, respectively in blank Pipe and standard pipe add 10ml deionized water and 10ml iron ion standard liquid, adds 0.2ml liquid-type iron ion detects reagent, 5-40 DEG C of reaction 5-15 minute;Bottom colorimetric cylinder Take color produced by each tube reaction to arsis, with photoshop 7.0 mapping software by each mark After quasi-pipe color deduction blank tube color, by color and the corresponding iron ion standard liquid of gained Concentration be printed upon on the water white transparency PVC plastic flitch that thickness is 1-2mm, obtain deduction examination Iron ion standard color card after agent is blank.
CN201510095223.5A 2015-03-04 2015-03-04 Detection kit for on-site quick detection of iron ions in water and preparing method Pending CN105987907A (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110470661A (en) * 2019-07-30 2019-11-19 北京倍肯恒业科技发展股份有限公司 The method and kit of thiocarbamide are remained in a kind of quick detection flour product

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110470661A (en) * 2019-07-30 2019-11-19 北京倍肯恒业科技发展股份有限公司 The method and kit of thiocarbamide are remained in a kind of quick detection flour product

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Application publication date: 20161005