CN105981806A - Fermented dairy product with addition of bovine coloctrum and preparation method thereof - Google Patents
Fermented dairy product with addition of bovine coloctrum and preparation method thereof Download PDFInfo
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- CN105981806A CN105981806A CN201510039064.7A CN201510039064A CN105981806A CN 105981806 A CN105981806 A CN 105981806A CN 201510039064 A CN201510039064 A CN 201510039064A CN 105981806 A CN105981806 A CN 105981806A
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Abstract
The invention provides a fermented dairy product with addition of bovine coloctrum and a preparation method thereof. The materials of the fermented dairy product include 5-20% of a sterilized bovine coloctrum solution, which is prepared by defatting the raw material, liquid bovine coloctrum, and then removing a part of casein and micro-filtering the bovine coloctrum. The fermented dairy product can be produced at room temperature or low temperature. The fermented dairy product avoids the degeneration and deactivation of immune globulin in the bovine coloctrum due to high-temperature sterilization during process. The content of the immune globulin is ensured well. The fermented dairy product is added with the sterilized and active bovine coloctrum which has active IgG, so that immune characteristic of the fermented dairy product is greatly improved.
Description
Technical field
The present invention relates to a kind of fermentation class milk product adding cattle colostrums and preparation method thereof, specifically utilize about one
Polypropylene filter is prepared sterile liquid cattle colostrums solution and adds the technology in fermentation class milk product to, belongs to milk and prepares
Field.
Background technology
Cattle colostrums is milk secreted in healthy cow farrows latter 72 hours, containing precious active immune globulin
And the nutrition such as abundant milk calcium matter, protein, various trace elements, it is described as " king of 21 century immunity ".
Compared with normal breast, its content content has dramatically different, and serum colostrum protein content is higher, and fat and sugar content is relatively low,
Iron content is 10～17 times of common milk, and vitamin D and A are respectively 3 times and 10 times of common milk.
Why cattle colostrums can cause everybody extensive concern, is because it natural containing the panimmunity factor, such as activity
Immunoglobulin, lactoferrin, lactoperoxidase, lysozyme etc., most important of which material is active immne ball
Albumen, its content is more than 10 times of normal Ruzhong.Cattle colostrums contains the immunoglobulin of five types, is respectively
IgA, IgD, IgE, IgM, IgG, wherein the content of IgG accounts for more than 80%, and characteristic is more steady than other four kinds
Fixed, it is topmost functional materials.Numerous studies and clinical trial prove that in cattle colostrums, IgG has immunomodulating merit
Can, can more efficiently prevent and improve disease, strengthen resistance against diseases, it is possible to suppression multiple pathogenic microorganisms.In addition cattle
Colostrum also has some other functions, such as enhancing development and raising IQ, health invigorating, raising exercise performance, disappears
Except tired, slow down aging, regulating intestinal canal flora etc..Cattle colostrums in 2000 by U.S. food science and technology association (IFT)
It is classified as 21 century non-medical herbs class natural health food most with prospects.
But, containing some sensitization sources in cattle colostrums, exist bigger potential particularly when be applied to infant food
Risk.Numerous studies show at present, and its sensitization source is primarily present in casein, if so by cheese egg in cattle colostrums
Its sensitization risk can be reduced significantly after white removing.On the other hand, owing in cattle colostrums, IgG is strong heat-sensitive materials,
When temperature is 40 DEG C of holding 30min, its inactivation rate can reach 95%, thus can not use the high temperature sterilize skill of routine
Art carries out sterilizing.These factors have impact on the use of cattle colostrums the most to a certain extent.It follows that eliminating at the beginning of cattle
Can guarantee that again the aseptic of cattle colostrums is particularly important on the premise of the potential sensitization source of breast, guarantee IgG activity.
For the solution containing heat-sensitive materials, membrane filtration is a kind of good degerming technique, particularly static filtering
(and flowing through filter), is not result in during membrane filtration heating up, without compromising on heat-sensitive materials, and micro-in solution simultaneously
Biological particle diameter is all higher than 0.2 μm, can reach complete bacteria-eliminating efficacy in the micro-filtration membrane through 0.2 μm.But at the beginning of cattle
The viscosity of breast solution is very big, and containing the material that more particle diameter is bigger, if directly being crossed film, membrane flux is very
Low.
Prior art use micro-filtrate membrane filtration technology cattle colostrums carries out the research report of sterilizing, wherein used
Micro-filtration membrane mostly is ceramic membrane.Such as, CN101263839A discloses with aperture 0.8～the ceramic mocromembrane pair of 1.4 microns
Milk containing cattle colostrums powder carries out filter sterilization.Document " the pollution resistance analysis of ceramic membrane filter cattle colostrums process "
(Wang Zhigao, Xing Weihong, Xu Nanping, " membrane science and technology ", in February, 2004, the 1st phase of volume 24) is investigated
The aperture impact on membrane filtration cattle colostrums process, analyzing aperture is 0.2 μm, 0.5 μm, 0.8 μm membrane filtration resistance
Being formed and on permeation flux and the impact of retention rate of proteins of power.Ceramic filtration membrane is with aluminium oxide, titanium oxide, oxidation
The material through special technology such as zirconium are prepared from, and its mechanical strength is big, and anti-pressure ability is strong, but, use ceramic filtration membrane
When carrying out microfiltration, operator scheme is usually cross-flow operation (i.e. dynamic filtration), has high tangential flow velocity, at high pressure
With cattle colostrums can be made under high speed conditions preferably to pass through, thus reached film effect.But meeting in filter process
Produce higher temperature, and cause IgG deactivation in cattle colostrums；Ceramic membrane cost is the highest simultaneously, simultaneously need to its
His auxiliary facility, makes the holistic cost when producing too high, is unfavorable for promoting.
CN 103039630A is disclosed at the beginning of a kind of aseptic cattle by membrane filtration technique preparation with innate immune activity
The method of breast milk surum, is wherein that cattle colostrums is removed by centrifugation fat, and uses isoelectric point method or curdled milk enzyme process precipitation
Casein, it is thus achieved that whey liqs first use the hollow-fibre membrane of aperture 0.4～1.2 μm or ceramic membrane to carry out pre-filtering,
Remove the fat of residual in milk surum, casein and major part thalline；The polytetrafluoroethyl-ne using aperture to be 0.1～0.4 μm again
Alkene film filter carries out filtration sterilization.Utilize the method, improve the treating capacity of feed liquid, can to a certain degree reduce IgG
Loss rate.But, in the method, pre-filtering is also to use dynamic filtration, as it was previously stated, tangential flow velocity can make cattle
Colostrum is preferably by filter membrane, but can heat up and cause IgG deactivation in cattle colostrums in filter process.It addition,
The method need to use the film of two kinds of unlike materials to filter cattle colostrums, and supporting filter apparatus is more, and hollow
Fibrous membrane, ceramic membrane, poly tetrafluoroethylene price are of a relatively high, and these all limit the actual application of this technology.
Additionally, document " impact that the pH value factor-1 (IGF-1) raw on Insulin-Like in cattle colostrums is isolated and purified " (Tian Xiao
Gorgeous, " food with biotechnology journal ", in July, 2005, the 4th phase of volume 24) research show, during ultrafiltration,
In the basic conditions, the permeability of IGF-1 strengthens.Think and be probably when pH value > 7 time, whey protein obtains
Clean negative charge, the electrostatic repulsion making molecule ask increases, and the cohesion that albumen is interrogated disappears, and transmitance has bigger
Increase.But, document filter membrane material the most used.
On the other hand, fermentation milk (being commonly called as " Yoghourt ") is with fresh milk as raw material, after pasteurize again
In milk, add probiotics (leaven), fermented after make.The sweat of fermentation milk makes sugar, egg in milk
White matter has about 20% to be hydrolyzed into as little molecule (such as galactose and lactic acid, little peptide chain and aminoacid etc.), milk
Middle fat content is usually 3%～5%.After fermented, the fatty acid in Ruzhong increases by 2 times than raw material milk, and these become
Changing and make Yoghourt more easy to digest and absorb, the utilization rate of various nutrients is improved.Fermentation milk is except remaining fresh milk
All outside nutritional labeling, lactic acid bacteria all right produces multivitamin necessary to human nutrition during the fermentation,
Such as VB1, VB2, VB6, VB12 etc..Sweat also creates some peculiar tastes in addition.Therefore, send out
Kefir milk becomes the food deeply liked by consumer in daily life.But the enhancing immunity that fermentation milk itself contains
Composition is little, if it is possible to is added to by cattle colostrums in fermentation milk and develops the fermentation class milk product made new advances, is greatly improved
The immunity of fermented dairy product.But, due to above-mentioned about without sensitization source and the sterile bovine colostrum of guarantee IgG activity
The shortcoming of production technology, limit its actual application in the fermentation class milk product adding cattle colostrums composition.Additionally,
How to ensure after being joined in fermented dairy product by cattle colostrums that its stability and good local flavor and mouthfeel are also to need to examine
The problem considered.
Summary of the invention
It is an object of the invention to develop a kind of fermentation class milk product adding cattle colostrums, this fermentation class milk product adds
Add the cattle colostrums component of aseptic and active IgG, substantially increase the immunological characteristic of fermentation class milk product.
Another object of the present invention is to provide the preparation method of the fermentation class milk product of described interpolation cattle colostrums.
For providing a kind of fermentation class milk product adding cattle colostrums, inventor is from preparing rich at the beginning of the aseptic cattle of IgG
Breast sets about studying, and finds in investigation, and polypropylene micro-filtration membrane is filter membrane conventional in a kind of food service industry, with food
Grade isotactic polypropylene is raw material, produces overall process without any additive；Physics, stable chemical performance, have well
The compatibility；Having the aperture of series, porosity is high, and pollutant holding capability is big, can recoil and high-temperature sterilization, inexpensive.Polypropylene
The microfiltration sterilization that filter membrane is widely used in food service industry, but prior art does not find polypropylene micro-filtration membrane
Report for cattle colostrums sterilization.Inventor analyzes its main cause and may is that, the polypropylene that market is extensively sold
Micro-filtration membrane mechanical strength is far short of what is expected compared to ceramic membrane, and particularly polypropylene micro-filtration membrane black brittleness is big, pressure scope
It is normally no higher than 3Bar, if using it for static filtering cattle colostrums, even if in advance cattle colostrums being carried out defat, taking off
Casein, at lower pressures, the emulsion after defat, de-casein is also difficult to pass through polypropylene micro-filtration membrane,
Membrane flux is the lowest.Inventor in further research by substantial amounts of grope test after find, de-by controlling
After fat, caseic content when emulsion being transferred under specific pH value in cattle colostrums emulsion, can significantly improve film and lead to
Amount, makes the cattle colostrums emulsion can be with at low pressures can be in static filtering mode smoothly by the polypropylene of 0.2 μm
Filter membrane.In gnotobasis, carry out aforesaid operations just can prepare aseptic cattle colostrums solution, immune globulin can be avoided
The deactivation that (predominantly IgG) causes because of high temperature in vain.Thus, the present invention successfully uses polypropylene filter pair
Cattle colostrums carries out microfiltration and has prepared sterile liquid cattle colostrums solution, it is possible to reduce the loss of IgG in the course of processing, and
It is prepared for adding the fermentation class milk product of cattle colostrums one of with gained sterile liquid cattle colostrums as raw material.
Specifically, on the one hand, the invention provides a kind of fermentation class milk product adding cattle colostrums, with this fermentation class
The gross weight meter of milk product, its dispensing includes the sterile bovine colostrum solution of 5%～20%, described sterile bovine colostrum solution
It is that raw material liquid cattle colostrums obtains through microfiltration after defat, removing part casein.
Cattle colostrums potential sensitization source can be eliminated as it was previously stated, a kind of and ensure obtaining of aseptic cattle colostrums active for IgG
Must be the present invention the fermentation class milk product and preparation method thereof adding cattle colostrums can conscientiously popularization and application key it
One.According to specific embodiments of the present invention, sterile bovine colostrum solution used in the present invention is to make in accordance with the following methods
For obtain:
By raw material liquid cattle colostrums defat；
It is albumen total content in emulsion that emulsion after defat removes casein content in part casein and control emulsion
10%～20%；
Remove the alkalescence salt regulation pH value of the emulsion after part casein to 4.5～5.5, pass sequentially through afterwards 0.8 μm,
0.45 μm, the polypropylene filter of 0.2 μm carry out microfiltration, and gained filtrate is sterile liquid cattle colostrums solution.
According to specific embodiments of the present invention, the sterile liquid cattle colostrums solution of the present invention is in preparation process, described
Microfiltration operator scheme is static filtering, i.e. and flow through filter, is not result in heating up, without compromising on heat during membrane filtration
Quick material.
According to specific embodiments of the present invention, the sterile liquid cattle colostrums solution of the present invention is in preparation process, described
Raw material liquid cattle colostrums is fresh cattle colostrums or restores the recovery emulsion obtained with cattle colostrums powder.Raw material liquid cattle colostrums
The usual content of middle IgG is 10～100mg/mL, is commonly 10～50mg/mL, with specific reference to cattle colostrums acquisition time
And batch and different.Preferably, the total solids content of raw material liquid cattle colostrums of the present invention is
13.0%～18.0%, fat content 3.0%～5.0%, total protein content 4.0%～6.0%, IgG content is
10～50mg/mL.
According to specific embodiments of the present invention, the sterile liquid cattle colostrums solution of the present invention is in preparation process, described
Skimming processes can be that the fat in cattle colostrums is all removed by the conventional degreasing process using art.
According to specific embodiments of the present invention, the sterile liquid cattle colostrums solution of the present invention is in preparation process, described
Remove the caseic process of part and include that the pH value of the emulsion after defat is transferred to 4.3～4.6 by the method using Acid precipitation,
By casein precipitate, sock filtration (preferably with 140～180 mesh filter bags) can be passed through afterwards and/or be centrifuged (such as
Centrifugal 20min～30min under the conditions of 3000rpm～4000rpm, low temperature such as 4～10 DEG C) mode that filters filters cheese egg
In vain.Owing to the isoelectric point, IP of the immunoglobulin IgG in cattle colostrums is meta-alkalescence, employing acid precipitation method will not when processing
Impacting its content, simultaneously compared with other caseic methods of precipitation, its cost is relatively low, and technique is relatively simple,
It is easy to actual production operation.Acid used in Acid precipitation method can be the one in lactic acid, acetic acid, citric acid or many
Several.In the present invention, do not pursue and casein is removed completely, but controlling casein content in filtrate is in emulsion
10wt%～20wt% of albumen total content.In addition to indicating especially, heretofore described percentage composition is all attached most importance to ratio
Amount percentage composition and part by weight.
According to specific embodiments of the present invention, the sterile liquid cattle colostrums solution of the present invention, in preparation process, regulates
One or more in sodium bicarbonate, potassium bicarbonate, sodium acetate, potassium acetate of alkalescence salt used during pH value,
Preferably sodium bicarbonate.When specifically applying, it is that the alkalescence saline solution using concentration 5%～15% preferably 5%～10% is adjusted
Joint pH value.The use of these alkalescence salt can reduce as far as possible IgG because of partial over-alkali produce precipitation or deactivation and
Cause IgG content to lose, and notable adverse effect will not be brought to crossing film effect.
According to specific embodiments of the present invention, the sterile liquid cattle colostrums solution of the present invention in preparation process, microfiltration
Time to control the mistake mould difference of each road film be 0.5～2Bar, i.e. the emulsion after regulation pH value is the pressure 0.5～2Bar
0.8 μm, 0.45 μm, the filter membrane of 0.2 μm is passed sequentially through under (both sides differential pressure of membrane).In filter process, each road film
Cross mould difference suitably to float in the range of 0.5～2Bar, but not can exceed that the pressure-bearing scope of polypropylene filter.
In the present invention, to micro-filtration used without particular/special requirement, such as, industrial conventional plate and frame can be used to filter
Device, it is possible to use other the microfiltration equipment that can realize pressure reduction static filtering operator scheme.
Should be understood that in the preparation method of sterile liquid cattle colostrums solution of the present invention, each step all should be at a lower temperature
Operation, to avoid in emulsion active substance because of the too high loss of temperature.Preferably, control whole during emulsion temperature not
Higher than 10 DEG C, more preferably 4～8 DEG C.
According to the method for the present invention preparation-obtained sterile liquid cattle colostrums solution, wherein, IgG content is
10～100mg/mL, usually 10～50mg/mL.Although the sterile liquid cattle colostrums solution of the present invention is in preparation process
In, the emulsion before membrane filtration remains a certain amount of casein, but tests sterile liquid obtained after proof filters
Cattle colostrums solution does not exist allergin.The technology of the present invention, passes through in static filtering mode making emulsion
While polypropylene filter, allergin that may be present in cattle colostrums removed, be substantially reduced its potential risk simultaneously,
And well ensure that the content of the functional component in cattle colostrums, particularly immunoglobulin IgG.
In the present invention, add described sterile liquid cattle colostrums solution to fermentation class milk product as one of raw material further
In, and prepare a kind of fermentation class milk product adding cattle colostrums.The fermentation class breast system adding cattle colostrums of the present invention
In product, in addition to the sterile liquid cattle colostrums solution added, other components can be according to the routine operation in art
Carry out.
According to specific embodiments of the present invention, the fermented dairy product of the present invention is with fresh milk or reconstituted milk for the most former
The milk of material (can be properly added stabilizer, sweeting agent etc.) is as fermentation substrate, through sterilization, inoculating starter strain
Ferment and make fermentation milk base material, then add what sterile bovine colostrum solution was prepared from.
Whether inactivate according to the fermentation milk base material after fermentation, the fermentation class milk product adding cattle colostrums of the present invention can be
Low temperature fermentation class milk product (active bacteria fermented dairy product, need cryopreservation) or normal temperature fermentation class milk product are (after fermentation
Through the fermented dairy product of inactivation, can preserve for a long time at room temperature).
In a preferred embodiment of the present invention, the fermentation class milk product of described interpolation cattle colostrums is normal temperature fermentation class breast
Goods, in terms of the gross weight of the class milk product that ferments, raw material composition (dispensing) of normal temperature fermentation class milk product includes: nothing
Bacterium cattle colostrums solution 5%～20%, stabilizer 1.5%～10%, leaven 100～300U, sweeting agent 0.05%～15%,
Milk surplus.
In another preferred embodiment of the present invention, the fermentation class milk product of described interpolation cattle colostrums is low temperature fermentation class
Milk product, in terms of the gross weight of the class milk product that ferments, the raw material composition of low temperature fermentation class milk product includes: at the beginning of aseptic cattle
Breast solution 5%～20%, stabilizer 3%～10%, leaven 100～300U, sweeting agent 0.05%～15%, more than milk
Amount.
It is appreciated that in the dispensing of the fermentation class milk product adding cattle colostrums of the present invention, sterile bovine colostrum solution is more
Specifically suitably can adjust according to the number of wherein IgG content.
According to specific embodiments of the present invention, sweet in the dispensing of the fermentation class milk product adding cattle colostrums of the present invention
Taste agent and stabilizer can select the conventional material in art.Preferably, in order to increase the fermentation adding cattle colostrums
The sweet taste of class milk product and mouthfeel, sweeting agent of the present invention is preferably white sugar, neotame, aspartame, cyclamate
With one or more in acesulfame potassium, the white sugar of preferably 5%～15%.In order to improve of the present invention adding further
Adding the stability of the normal-temperature yoghourt goods of cattle colostrums, described stabilizer is thickening agent and/or emulsifying agent, and thickening agent is preferred
For the one in carboxymethyl cellulose, really Konjac glucomannan, carrageenan, dextrin, xanthan gum, microcrystalline Cellulose, pectin or
Multiple；Emulsifying agent is preferably glyceryl monostearate, sucrose fatty acid ester, sodium stearoyl lactate, double glycerol stearate
One or more in ester and succinic acid monoglyceride；In order to improve the low temperature acid of interpolation cattle colostrums of the present invention further
The stability of milk product, described stabilizer is thickening agent, and thickening agent is preferably carboxymethyl cellulose, really Konjac glucomannan, card
Draw one or more in glue, dextrin, xanthan gum, gelatin, pectin.The concrete consumption of sweeting agent and stabilizer is permissible
With reference to the prior art of art or carry out simple experiment and determine.
According to specific embodiments of the present invention, milk used in dispensing of the present invention should meet wanting of convenient source breast
Ask, wherein albumen >=3.0 (g/100g), dry >=12.5 (g/100g), obtain as detecting instrument using 120 full milk analysers
To result be as the criterion, remainder should meet the requirement of GB 19301-2010.Specifically, described milk is full-cream cattle
Milk, skim milk or fat content are 2.0 (g/100g)～the milk of 3.0 (g/100g), can be Fresh Milks, or
With the formulated recombined milk of other components of milk powder, butter, hydrolyzed protein, whey powder or milk.
According to specific embodiments of the present invention, leaven used in dispensing of the present invention can also select art
Conventional starter culture.Preferably, described leaven include Lactobacillus bulgaricus (Lactobacillus bulgaricus),
Streptococcus thermophilus (Streptococcus thermophilus), bacillus acidophilus (Lactobacillus acidophilus),
Bacillus bifidus (Bifidobacterium), lactobacillus casei (Lactobacillus casei) and lactobacillus rhamnosus
The combination of one or more in (Lactobacillus GG) etc..
Additionally, the fermentation class milk product adding cattle colostrums of the present invention, it is possible to it is added as needed on appropriate essence etc.
The convenient source in fermented dairy product field.
On the other hand, present invention also offers the preparation method of the described fermentation class milk product adding cattle colostrums, the party
Method includes:
Can cryopreservation or normal with other raw materials preparation except sterile bovine colostrum solution in addition in fermentation class milk product dispensing
The fermentation milk base material that temperature preserves；
Utilize in the above-mentioned fermentation milk base material that sterile bovine colostrum solution is added to less than 25 DEG C by online addition manner, prepare
Add the fermentation class milk product of cattle colostrums.
In the method for the fermentation class milk product that cattle colostrums is added in the preparation of the present invention, if preparation normal temperature fermentation breast system
Product, it is necessary to the fermentation milk of (being preferably cooled to less than 25 DEG C, be more preferably cooled to less than 10 DEG C) after sterilization, cooling
Aseptic online interpolation cattle colostrums solution in base material, to ensure that IgG therein is retained in fermentation milk finished product to greatest extent；
If preparing viable bacteria fermentation milk product, it is also necessary to be after fermentation ends, after cooling (preferably cool to less than 25 DEG C,
More preferably it is cooled to less than 10 DEG C) the most aseptic interpolation cattle colostrums solution.
According to specific embodiments of the present invention, the preparation method of the fermentation class milk product adding cattle colostrums of the present invention is also
Sterile bovine colostrum solution is obtained including carrying out microfiltration again after raw material liquid cattle colostrums being carried out defat, removing part casein
Process.The more specifically preparation process of sterile bovine colostrum solution sees above description.
In the preparation method of the fermentation class milk product adding cattle colostrums of the present invention, the step mentioned the most in detail, such as have
The preparation process of the fermentation milk base material of body (include dispensing, sterilize, inoculate, fermentation etc.), online adding procedure etc. can
Carry out with the routine operation with reference to art.
In sum, the technology of the application present invention, reach the same of aseptic result making obtained liquid bovine colostrum solution
Time, it is possible to well preserving the active immne material IgG in cattle colostrums, the present invention provides a kind of interpolation at the beginning of cattle according to this
The room temperature of breast or low temperature fermentation class milk product, with nutritious daily food materials as raw material, in enhancing immunity, prevention
Substantial amounts of nutritional labeling is provided while disease, is eaten for a long time and body will not be produced any side effect, expanded at the beginning of cattle
The range of breast.
Detailed description of the invention
In order to the technical characteristic of the present invention, purpose and beneficial effect are more clearly understood from, in conjunction with instantiation
Technical scheme is carried out described further below, it should be understood that these examples be merely to illustrate the present invention rather than
Limit the scope of the present invention.
The total solid preparing raw material liquid cattle colostrums used in the embodiment 1～4 of sterile liquid cattle colostrums solution contains
Amount is 15.0%, fat content 4.0%, total protein content 4.5%, and IgG content is 13.5mg/mL；Used
Microfiltration equipment is flat microstrainer, and effective membrane area (monofilm) of crossing is 69.36cm2.Each embodiment carries the most in detail
And step all can carry out according to the routine operation of art.
Embodiment 1
Present embodiments providing a kind of sterile liquid cattle colostrums solution, it prepares according to following steps:
1, defat: use conventional degreasing process all to be removed by the fat in fresh for raw material cattle colostrums.
2, precipitation casein: use the method for Acid precipitation the pH value of cattle colostrums to be transferred to 4.3, by casein precipitate.
3, filter: remove roughly casein with 140 mesh filter bags, obtain filtrate.This filtrate through cross mould difference 2Bar,
Static filtering pattern descended 0.45 μm and the test of 0.2 μm polypropylene filter, in initial 1 minute, by the breast of filter membrane
Liquid is less than 20g.
It is 4, centrifugal: filtrate above-mentioned steps 3 obtained is centrifugal 30min under the conditions of 4000rpm, removes precipitation,
Obtaining supernatant, in this supernatant, casein content is about the 10% of total protein content.
5, pH: the potassium bicarbonate with 8% is adjusted to be adjusted making its pH value reach 4.5 to step 4 gained supernatant.
6, the preparation of gnotobasis: the polypropylene filter of microfiltration equipment and 0.8 μm, 0.45 μm and 0.2 μm is put
Entering in super-clean bench, ultra-vioket radiation 1h, to reach aseptic result.
7, film is crossed: added by the supernatant mixing up pH value in step 5 in the microfiltration equipment of step 6 so that it is at 2Bar
Pass sequentially through 0.8 μm, 0.45 μm, the polypropylene filter of 0.2 μm under the pressure of left and right, afterwards solution is stored in nothing
In nectar envelope, obtain sterile liquid cattle colostrums solution, standby.
The film effect excessively of the present embodiment is as follows:
In the case of not changing filter membrane, prepare 1000g sterile liquid cattle colostrums solution and take about 17.3min.
Embodiment 2
Present embodiments providing a kind of sterile liquid cattle colostrums solution, it prepares according to following steps:
1, defat: use conventional degreasing process all to be removed by the fat in cattle colostrums.
2, precipitation casein: use the method for Acid precipitation the pH value of cattle colostrums to be transferred to 4.5, by casein precipitate.
3, filter: remove roughly casein with 180 mesh filter bags, obtain filtrate.This filtrate through cross mould difference 2Bar,
Static filtering pattern descended 0.45 μm polypropylene filter test, in initial 1 minute, by the emulsion of filter membrane less than 20g.
It is 4, centrifugal: by above-mentioned solution centrifugal 20min under the conditions of 3000rpm, to remove precipitation, obtain supernatant,
In this supernatant, casein content is the 20% of total protein content.
5, adjusting pH: the potassium acetate with 8% that the supernatant obtained by step 4 carries out acid adjustment makes its pH value reach 5.0.
6, the preparation of gnotobasis: the polypropylene filter crossing film device and 0.8 μm, 0.45 μm and 0.2 μm is put
Entering in super-clean bench, ultra-vioket radiation 1h, to reach aseptic result.
7, mistake film: the supernatant mixing up pH value in step 5 is added in the film device excessively of step 6 so that it is
0.8 μm, 0.45 μm, the polypropylene filter of 0.2 μm is passed sequentially through, afterwards by solution under the mistake mould difference of about 2Bar
It is stored in sterile sealing bag, obtains sterile liquid cattle colostrums solution.
The film effect excessively of the present embodiment is as follows:
In the case of not changing filter membrane, prepare 1000g sterile liquid cattle colostrums solution and take about 20.1min.
Embodiment 3
Present embodiments providing a kind of sterile liquid cattle colostrums solution, it prepares according to following steps:
1, defat: use conventional degreasing process all to be removed by the fat in cattle colostrums.
2, precipitation casein: use the method for Acid precipitation the pH value of cattle colostrums to be transferred to 4.3, by casein precipitate.
3, filter: remove roughly part casein with 140 mesh filter bags, obtain filtrate.
It is 4, centrifugal: by above-mentioned filtrate centrifugal 25min under the conditions of 4000rpm, to remove precipitation, obtain supernatant,
In this supernatant, casein content is about the 13% of total protein content.
5, pH is adjusted: the supernatant obtained by step 4 is adjusted by the sodium acetate with 8% so that it is pH value reaches
5.5。
6, the preparation of gnotobasis: film device and 0.8 μm, 0.45 μm and 0.2 μm polypropylene filter excessively are put into
In super-clean bench, ultra-vioket radiation 1h, to reach aseptic result.
7, film is crossed: added in film device by the supernatant mixing up pH value in step 5 so that it is at about 1.5Bar
Mistake mould difference under pass sequentially through 0.8 μm, 0.45 μm, the filter membrane of 0.2 μm, afterwards solution is stored in sterile sealing
In Dai, obtain sterile liquid cattle colostrums solution.
In the present embodiment, in the case of not changing filter membrane, prepare 1000g sterile liquid cattle colostrums solution and take about
24.3min。
Embodiment 4
Present embodiments providing a kind of sterile liquid cattle colostrums solution, it prepares according to following steps:
1, defat: use conventional degreasing process all to be removed by the fat in cattle colostrums.
2, precipitation casein: use the method for Acid precipitation the pH value of cattle colostrums to be transferred to 4.5, by casein precipitate.
3, filter: remove roughly casein with 140 mesh filter bags, obtain filtrate.
It is 4, centrifugal: by above-mentioned filtrate centrifugal 20min under the conditions of 4000rpm, to remove precipitation, obtain supernatant,
Casein content about 15% in this supernatant.
5, acid adjustment: the potassium bicarbonate with 5% carries out acid adjustment to the supernatant obtained by step 4 so that it is pH value reaches
4.5。
6, the preparation of gnotobasis: film device and 0.8 μm, 0.45 μm and 0.2 μm polypropylene filter excessively are put into
In super-clean bench, ultra-vioket radiation 1h, to reach aseptic result.
7, film is crossed: added in film device by the supernatant mixing up pH value in step 5 so that it is in the mistake of about 2Bar
Pass sequentially through 0.8 μm, 0.45 μm, the filter membrane of 0.2 μm under mould difference, afterwards solution be stored in sterile sealing bag,
Obtain sterile liquid cattle colostrums solution.
In the present embodiment, in the case of not changing filter membrane, prepare 1000g sterile liquid cattle colostrums solution and take about
23.8min。
The investigation of IgG content in each embodiment product
After the various embodiments described above make sterile liquid cattle colostrums product, the content of IgG in product is detected,
(for ease of comparing, in table 1, detected value is to be converted into the sterile liquid cattle colostrums of each embodiment with former to the results are shown in Table 1
Numerical value after material liquid bovine colostrum volume is identical).
The mensuration of IgG content in the different embodiment of table 1
| IgG content (mg/mL) | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 |
| Theoretical value | 13.5 | 13.5 | 13.5 | 13.5 |
| Detected value | 13.03 | 12.83 | 12.93 | 12.97 |
| Loss amount | 3.48% | 4.96% | 4.22% | 3.93% |
As shown in Table 1, in various embodiments of the present invention, the loss amount of IgG is respectively 3.48%, 4.96%, 4.22%, 3.93%,
All < 5%, it is preferable that the method prepares sterile liquid cattle colostrums product effect to loss amount.
Under condition of different pH, solution passes through filter membrane effect expedition
Take five parts of 1000g raw materials, on the premise of other condition is identical by pH in step 6 for not being adjusted to 4.5,5.0,
5.5,6.0,6.5, investigate the film effect excessively by 0.2 μm filter membrane respectively, be specifically shown in Table 2.
The different pH value of table 2 must be investigated
| Scheme one | Scheme two | Scheme three | Scheme four | Scheme five | |
| Material liquid pH value | 4.5 | 5.0 | 5.5 | 6.0 | 6.5 |
| Use diaphragm number | 1 | 1 | 1 | 4 | 6 |
| Required time | 24.3min | 26.1min | 24.7min | 189min | 308min |
As shown in Table 2, under conditions of pH4.5～5.5, it is the shortest that the cattle colostrums solution of equal number crosses film required time,
And only need 1 filter membrane, and when the solution of 1000g pH6.0 crosses film, need to change 4 filter membranes, 1000g pH6.5
Solution need to change 6 filter membranes (being that every 1h changes a film in scheme four, scheme five) when crossing film.
The investigation of bacteria-eliminating efficacy
Sterile liquid cattle colostrums solution 1mL in the step 7 of Example 1～4 is inoculated into and total plate count is surveyed respectively
In test piece and mycete yeast test sheet, total plate count being tested sheet and is put in 37 DEG C of baking ovens cultivation 48h, mycete yeast is surveyed
Cultivation 120h in 25 DEG C of baking ovens is put in test piece, takes out afterwards and sees whether long bacterium.Specifically it is shown in Table 3.
The investigation of table 3 bacteria-eliminating efficacy
As shown in Table 3, the sterile liquid cattle colostrums solution in various embodiments of the present invention is equal asepsis growth in 6 months,
Bacteria-eliminating efficacy is good.
Anaphylaxis is investigated
By the sterile liquid cattle colostrums solution of embodiment 1～embodiment 4, with complete defat removing at the beginning of caseic cattle
Emulsion, only defat and not removed caseic cattle colostrums, as test sample, enter with 72 mices with a collection of health
Row experiment, is divided into five groups, often group 12, often organizes and carries out feeding with above-mentioned cattle colostrums solution respectively, respectively feeds one
Secondary, each 100g, feed 30 days, mice health condition of detection in every 3 days, to investigate the anaphylaxis of product,
The health condition content of mice is shown in Table 4.
Table 4 mice health condition is investigated
As shown in Table 4, during with the cattle colostrums solution feeding mice of various embodiments of the present invention, mice health condition is good,
There is no malaise symptoms, illustrate that the cattle colostrums after this PROCESS FOR TREATMENT does not contains sensitinogen, can eat for human body.
The normal-temperature yoghourt embodiment of interpolation cattle colostrums:
Embodiment 5 adds the normal-temperature yoghourt goods of cattle colostrums
Formula for a product (in terms of 1000kg product, raw materials used selection meets concerned countries standard)
The method preparing the normal-temperature yoghourt of above-mentioned interpolation cattle colostrums, comprises the following steps:
1, raw milk sterilization processing: it is two BB centrifuge tandem process combinations that required milk is taked bactofugation
Being centrifuged degerming, degerming temperature is 55～65 DEG C, and rotating speed is 4200～6300 revs/min, carries out raw milk
Sterilizing；
2, the raw milk after above-mentioned sterilization is heated to 50～55 DEG C, mixes under high velocity agitation with stabilizer, sweeting agent
Close 15～20 minutes, feed liquid is carried out full homogenizing (homogenizing temperature is 55～75 DEG C, pressure 180～200bar/36～50bar),
Sterilization treatment before fermentation, obtains the bed material that ferments；When containing raw materials such as essence, together can add with stabilizer,
Can also be added the most again；
3, above-mentioned fermentation bed material is cooled to 42～43 DEG C, put into strain, 42 DEG C insulation standing for fermentation, to Titrable acid
Degree reaches to terminate fermentation during 70 ° of T～90 ° of T, stirs breakdown of emulsion (stirring speed after being then rapidly cooled to 20 DEG C～30 DEG C
Degree is 65～100 revs/min, and mixing time is 25～30 minutes)；
4, (sterilization pressure is 500～650MPa, sterilization time the above-mentioned fermentation liquid being stirred to be carried out autoclaving
It is 15～45min), or (72～121 DEG C, sterilizing time is 4 seconds～10 to use board-like sterilization machine or tubular sterilization machine
Minute) carry out sterilization processing, it is cooled to less than 25 DEG C after sterilization；
5, add to the most aseptic for sterile liquid cattle colostrums solution in fermentation liquid；
6, sterile filling, obtain can room temperature preserve fermented dairy product；
7, vanning is dispatched from the factory: canned milk carries out coding, patch pipe；Dispatch from the factory after warm experimental examination is qualified.
Embodiment 6 adds the normal-temperature yoghourt goods of cattle colostrums
Formula for a product (in terms of 1000kg product, raw materials used selection meets concerned countries standard)
Preparation method is with embodiment 5.
Embodiment 7 adds the normal-temperature yoghourt goods of cattle colostrums
Formula for a product (in terms of 1000kg product, raw materials used selection meets concerned countries standard)
Preparation method is with embodiment 5.
Embodiment 8 adds the normal-temperature yoghourt goods of cattle colostrums
Formula for a product (in terms of 1000kg product, raw materials used selection meets concerned countries standard)
Preparation method is with embodiment 5.
The low-temperature yoghurt Articles Example of interpolation cattle colostrums:
Embodiment 9 adds the low-temperature yoghurt goods of cattle colostrums
Formula for a product (in terms of 1000kg product, raw materials used selection meets concerned countries standard)
The method preparing the low-temperature yoghurt of above-mentioned interpolation cattle colostrums, comprises the following steps:
1, batch mixing: after white sugar and stabilizer mix homogeneously, obtain compound powder；Then by described compound
Powder joins in a part of milk being preheated to 60 ± 1 DEG C, carries out shear-mixed, the shearing of described shear-mixed simultaneously
Speed is 1300rpm～1800rpm (most preferably 1500rpm), and the shear-mixed time is 20～35min, is cutting
Cut mixed process keeps temperature of charge at 60 ± 1 DEG C, feed liquid after obtaining mixing for the first time；
2, constant volume: remaining milk is added, carries out constant volume；
3, homogenizing: feed liquid feed liquid good for constant volume is carried out homogenizing under conditions of 40～85 DEG C, 15～20MPa；
4, sterilize and cool down: after described third time being mixed, feed liquid is at 90 DEG C of 10min that sterilize, and is subsequently cooled to 42 DEG C,
Feed liquid after being sterilized；
5, ferment and cool down: feed liquid being added after described sterilization leaven and carries out standing for fermentation, reach to titratable acidity
Terminate fermentation, breakdown of emulsion after being then rapidly cooled to 20 DEG C～30 DEG C during to 70 ° of T～90 ° of T, obtain Yoghourt base material；
6, cattle colostrums is added online: under aseptic condition, add sterile liquid cattle colostrums solution to above-mentioned acid online
In milk base material, obtain adding the fermentation emulsion of cattle colostrums；
7, fill and cold preservation after-ripening: the fermentation emulsion of described interpolation cattle colostrums is carried out sterile filling, warehouse-in, cold
Hide after-ripening, obtain the fermented dairy product of described interpolation cattle colostrums；
8, vanning is dispatched from the factory: canned milk carries out coding, patch pipe；Dispatch from the factory after warm experimental examination is qualified.
Embodiment 10 adds the low-temperature yoghurt goods of cattle colostrums
Formula for a product (in terms of 1000kg product, raw materials used selection meets concerned countries standard)
Preparation method is with embodiment 9.
Embodiment 11 adds the low-temperature yoghurt goods of cattle colostrums
Formula for a product (in terms of 1000kg product, raw materials used selection meets concerned countries standard)
Preparation method is with embodiment 9.
Embodiment 12 adds the low-temperature yoghurt goods of cattle colostrums
Formula for a product (in terms of 1000kg product, raw materials used selection meets concerned countries standard)
Preparation method is with embodiment 9.
Sense organ and flavor evaluation experiment
Respectively to add the product of the embodiment 5～12 of the normal-temperature yoghourt of cattle colostrums and low-temperature yoghurt and common room temperature
Yoghourt and General low temperature Yoghourt, as test sample, carry out sense organ and flavor evaluation experiment.Normal-temperature yoghourt is every one
Carrying out sample sense organ and local flavor contrast the moon, the time is six months.Low-temperature yoghurt preserves to enter every five days under the conditions of 8 DEG C
Row sample sense organ and local flavor contrast, one month time.Sense organ and flavor evaluation experimental result are as shown in table 5, table 6.
Table 5 normal-temperature yoghourt each embodiment different time sense organ and local flavor comparing result
Table 6 low-temperature yoghurt each embodiment different time sense organ and local flavor comparing result
From table 5, the experimental result of table 6, the normal-temperature yoghourt goods adding cattle colostrums of the present invention can be at room temperature
Under the conditions of preserve more than 6 months, low-temperature yoghurt goods can preserve more than 30 days under the conditions of 8 DEG C, occurs without any
Abnormal flavour and color change, do not produce brown stain, beneficially product promotion and sale.
Stabiliser system is on product stability impact experiment
Under respective storage condition, the sample stability of the embodiment 5～12 of room temperature and low-temperature yoghurt goods is entered respectively
Row is investigated, and the investigation time is room temperature 6 months, low temperature 30 days, the results are shown in Table 7, table 8.
The investigation of table 7 normal-temperature yoghourt goods each embodiment product stability
The investigation of table 8 low-temperature yoghurt each embodiment product stability
From table 7 and table 8, the stabilizer stability in the present invention is good, and normal-temperature yoghourt is deposited at normal temperatures
6 months stable systems, low-temperature yoghurt preserves 30 days stable systems under the conditions of 8 DEG C, can meet needs.
The mensuration of product I gG changes of contents
Respectively the content of IgG in embodiment 5～12 sample in room temperature and low-temperature yoghurt goods is investigated, often
The time investigated by temperature Yoghourt is 6 months, and it is 30 days that low-temperature yoghurt investigates the time, the results are shown in Table 9, table 10.
The investigation of IgG content (mg/mL) in table 9 normal-temperature yoghourt goods each embodiment product
| Time | Embodiment 5 | Embodiment 6 | Embodiment 7 | Embodiment 8 |
| One month | 0.565 | 1.195 | 1.975 | 2.441 |
| Two months | 0.557 | 1.184 | 1.954 | 2.430 |
| Three months | 0.542 | 1.177 | 1.922 | 2.411 |
| Four months | 0.531 | 1.162 | 1.903 | 2.389 |
| Five months | 0.524 | 1.141 | 1.891 | 2.367 |
| Six months | 0.519 | 1.129 | 1.884 | 2.351 |
The investigation of IgG content (mg/mL) in table 10 low-temperature yoghurt each embodiment product
| Time | Embodiment 9 | Embodiment 10 | Embodiment 11 | Embodiment 12 |
| Five days | 0.564 | 1.210 | 1.957 | 2.499 |
| Ten days | 0.559 | 1.199 | 1.951 | 2.494 |
| 15 days | 0.551 | 1.187 | 1.942 | 2.487 |
| 20 days | 0.545 | 1.181 | 1.935 | 2.481 |
| 25 days | 0.541 | 1.174 | 1.927 | 2.472 |
| 30 days | 0.536 | 1.169 | 1.920 | 2.465 |
From table 9, table 10, in each embodiment product, IgG content loss is seldom.
The investigation of product aseptic
The product provided embodiment 5～12 in room temperature and low-temperature yoghurt goods respectively carries out aseptic investigation, room temperature
Yoghourt probation is six months, and low-temperature yoghurt probation is 30 days.Concrete grammar is as follows: take 1mL respectively in fact
The sample executed in example is inoculated in total plate count test sheet and mycete yeast test sheet under aseptic condition, and bacterium colony is total
Number test sheet is put in 37 DEG C of baking ovens cultivation 48h, and mycete yeast test sheet puts into cultivation 120h in 25 DEG C of baking ovens, it
Rear taking-up sees whether long bacterium.The results are shown in Table 11, table 12.
Table 11 normal-temperature yoghourt goods each embodiment aseptic is investigated
Table 12 low-temperature yoghurt goods each embodiment aseptic is investigated
From table 11, table 12, aseptic interior at six months of the product in each embodiment of normal-temperature yoghourt in the present invention
Property good, low-temperature yoghurt aseptic in 30 days is good, illustrates that the product that the present invention prepares is aseptic, also says
The cattle colostrums prepared in the bright present invention is aseptic, meets safety need.
Claims (10)
1. add a fermentation class milk product for cattle colostrums, in terms of the gross weight of this fermentation class milk product, in its dispensing
Including the sterile bovine colostrum solution of 5%～20%, described sterile bovine colostrum solution be raw material liquid cattle colostrums through defat, remove
Obtain through microfiltration after removing part casein.
The fermentation class milk product of interpolation cattle colostrums the most according to claim 1, this fermentation class milk product is room temperature
Fermentation class milk product or low temperature fermentation class milk product；
Preferably, in terms of the gross weight of the class milk product that ferments, the raw material composition of normal temperature fermentation class milk product includes: aseptic
Cattle colostrums solution 5%～20%, stabilizer 1.5%～10%, leaven 100～300U, sweeting agent 0.05%～15%,
Milk surplus；
In terms of the gross weight of fermentation class milk product, the raw material composition of low temperature fermentation class milk product includes: sterile bovine colostrum is molten
Liquid 5%～20%, stabilizer 3%～10%, leaven 100～300U, sweeting agent 0.05%～15%, milk surplus.
The fermentation class milk product of interpolation cattle colostrums the most according to claim 1, wherein, described sterile bovine colostrum
In solution, IgG content is 10～100mg/mL, preferably 10～50mg/mL.
The fermentation class milk product of interpolation cattle colostrums the most according to claim 1, wherein, described sterile bovine colostrum
Solution prepares in accordance with the following methods:
By raw material liquid cattle colostrums defat；
It is albumen total content in emulsion that emulsion after defat removes casein content in part casein and control emulsion
10%～20%；
Remove the alkalescence salt regulation pH value of the emulsion after part casein to 4.5～5.5, pass sequentially through afterwards 0.8 μm,
0.45 μm, the polypropylene filter of 0.2 μm carry out microfiltration, and gained filtrate is sterile liquid cattle colostrums solution.
5. according to the fermentation class milk product adding cattle colostrums described in claim 1 or 4, wherein, described material liquid
State cattle colostrums is fresh cattle colostrums or restores the recovery emulsion obtained with cattle colostrums powder.
6. according to the fermentation class milk product adding cattle colostrums described in claim 1 or 4, wherein, described removing unit
The pH value of the emulsion after defat is transferred to 4.3～4.6, by cheese egg by the method using Acid precipitation to divide caseic process to include
White precipitation, afterwards with 140～180 mesh sock filtration or combine the 3000～4000rpm modes being centrifuged, removing unit
Divide casein.
The fermentation class milk product of interpolation cattle colostrums the most according to claim 4, wherein, sterile liquid cattle colostrums
Solution, in preparation process, regulates the alkalescence saline that alkalescence salt is mass concentration 5%～15% used during pH value
Solution, one or more in sodium bicarbonate, potassium bicarbonate, sodium acetate, potassium acetate of alkalescence salt therein,
Preferably sodium bicarbonate.
The fermentation class milk product of interpolation cattle colostrums the most according to claim 4, wherein, sterile liquid cattle colostrums
Solution in preparation process, regulation pH value after emulsion be pass sequentially through under the pressure of 0.5～2Bar 0.8 μm,
0.45 μm, the polypropylene filter of 0.2 μm；Described microfiltration operator scheme is static filtering.
9. the preparation method of the fermentation class milk product adding cattle colostrums according to any one of claim 1～8, the method
Including:
Can cryopreservation or normal with other raw materials preparation except sterile bovine colostrum solution in addition in fermentation class milk product dispensing
The fermentation milk base material that temperature preserves；
Utilize in the above-mentioned fermentation milk base material that sterile bovine colostrum solution is added to less than 25 DEG C by online addition manner, prepare
Add the fermentation class milk product of cattle colostrums.
Method the most according to claim 9, the method also includes raw material liquid cattle colostrums carrying out defat, removing
Carry out microfiltration after removing part casein again and obtain the process of sterile bovine colostrum solution.
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