CN105973887A - Chip for rapidly detecting ischemia modified albumin in blood - Google Patents
Chip for rapidly detecting ischemia modified albumin in blood Download PDFInfo
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- CN105973887A CN105973887A CN201610579482.XA CN201610579482A CN105973887A CN 105973887 A CN105973887 A CN 105973887A CN 201610579482 A CN201610579482 A CN 201610579482A CN 105973887 A CN105973887 A CN 105973887A
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- modified albumin
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- 210000004369 blood Anatomy 0.000 title claims abstract description 30
- 239000008280 blood Substances 0.000 title claims abstract description 29
- 108010023015 ischemia-modified albumin Proteins 0.000 title claims abstract description 28
- 238000001514 detection method Methods 0.000 claims abstract description 92
- 239000010941 cobalt Substances 0.000 claims abstract description 54
- 229910017052 cobalt Inorganic materials 0.000 claims abstract description 54
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims abstract description 52
- 239000000126 substance Substances 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000000835 fiber Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- LNETULKMXZVUST-UHFFFAOYSA-N 1-naphthoic acid Chemical compound C1=CC=C2C(C(=O)O)=CC=CC2=C1 LNETULKMXZVUST-UHFFFAOYSA-N 0.000 claims description 4
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 4
- KTVIXTQDYHMGHF-UHFFFAOYSA-L cobalt(2+) sulfate Chemical compound [Co+2].[O-]S([O-])(=O)=O KTVIXTQDYHMGHF-UHFFFAOYSA-L 0.000 claims description 4
- 229940097267 cobaltous chloride Drugs 0.000 claims description 4
- VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 claims description 4
- 229910017604 nitric acid Inorganic materials 0.000 claims description 4
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000036541 health Effects 0.000 abstract description 5
- 230000002745 absorbent Effects 0.000 abstract 2
- 239000002250 absorbent Substances 0.000 abstract 2
- 239000000523 sample Substances 0.000 description 74
- 238000012360 testing method Methods 0.000 description 24
- 238000000034 method Methods 0.000 description 15
- 208000004476 Acute Coronary Syndrome Diseases 0.000 description 13
- 208000031225 myocardial ischemia Diseases 0.000 description 12
- 201000011244 Acrocallosal syndrome Diseases 0.000 description 11
- 208000019905 acrocephalosyndactyly Diseases 0.000 description 11
- 206010051895 acute chest syndrome Diseases 0.000 description 11
- 230000008859 change Effects 0.000 description 9
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 description 6
- 229910001429 cobalt ion Inorganic materials 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 5
- 102000001554 Hemoglobins Human genes 0.000 description 4
- 108010054147 Hemoglobins Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000004737 colorimetric analysis Methods 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 208000028867 ischemia Diseases 0.000 description 4
- 210000004165 myocardium Anatomy 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 108091006905 Human Serum Albumin Proteins 0.000 description 3
- 102000008100 Human Serum Albumin Human genes 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000013207 serial dilution Methods 0.000 description 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
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- 238000002360 preparation method Methods 0.000 description 2
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- 239000002994 raw material Substances 0.000 description 2
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- 238000007789 sealing Methods 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 239000012209 synthetic fiber Substances 0.000 description 2
- 229920002994 synthetic fiber Polymers 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 206010021703 Indifference Diseases 0.000 description 1
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- 229910052802 copper Inorganic materials 0.000 description 1
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- 210000004351 coronary vessel Anatomy 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
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- 238000013461 design Methods 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
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- 230000008588 hemolysis Effects 0.000 description 1
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- 230000000302 ischemic effect Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
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- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003950 pathogenic mechanism Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 235000021395 porridge Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- -1 sulfur threoses Chemical class 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- 229910001428 transition metal ion Inorganic materials 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a chip for rapidly detecting ischemia modified albumin in blood. The chip comprises a non-absorbent bottom plate and a non-absorbent cover plate, wherein a sample tank, a mixing tank and a detection tank are arranged on the bottom plate in sequence; micro-pipelines are arranged between the sample tank and the mixing tank and between the mixing tank and the detection tank; the sample tank is internally filled with a sample pad; the sample pad is coated with a cobalt standard product; the detection tank is internally filled with a detection pad; the detection pad is coated with a cobalt indicator; the cover plate is fixedly arranged above the bottom plate; and the cover plate is located above the sample tank and is provided with a sample feeding hole. The chip disclosed by the invention is simple in detection method, and a result is easy to judge; and the chip is suitable for being used in medical units including primary-level hospitals, emergency departments and the like or on bedsides of hospitalized patients, so that medical workers can rapidly judge dangers of the patients by layers and medical resources and health cost are saved.
Description
Technical field
The invention belongs to vitro detection technical field, be specifically related to ischemia in a kind of quickly detection blood and repair
Adorn albuminous chip.
Background technology
Acute coronary syndrome (acute coronary syndrome, ACS) is the eighties in 20th century
Since the diagnosis new ideas of coronary atherosclerotic heart disease that propose.Hard with coronary artery medicated porridge sample
Change Plaque instability is basic pathology, physiological characteristics, with acute myocardial ischemia as common trait
Group disease group.ACS is the modal cardiovascular disease of the mankind.China increases ACS the most every year newly and suffers from
Person about more than 1,000,000, and ACS accounts for more than the 10% of total population disease death reason, and ACS has become
For endangering the common disease of people's health.Mistaken diagnosis or delay diagnosis cause not only to patient mental and health
Injury, returns society's public health resources and causes loss greatly and waste.
Myocardial ischemia is the pathomechanism causing ACS disease group.Morrow etc. point out: the preferably heart
Myocardial ischemia mark should possess following characteristics: (1) sensitivity and specificity are the highest, it is necessary to can examine
Thought-read myocardial ischemia, but do not increase during healthy individuals, inflammation and other organ injury；(2)
Should be able to detect in early days during myocardial ischemia, and be directly proportional to the scope of involvement of myocardium；(3) exist
Good stability in circulation, sustainable detection is to keep enough period；Blood in (4) 24 hours
Middle concentration returns to foundation level, in order to the characteristics such as detection recurrent ischemia.
Research finds, Ischemia modified albumin IMA (Ischemia modified albumin, IMA) is anti-
Reflect the mark of myocardial ischemia in early days.As the specific parameters of cardiac muscle ischemia in early days, IMA is real in clinic
Good directive function it is demonstrated by, its detection method in trampling--Cobalt binding test (albumin-cobalt
Binding, ACB) it is that U.S. FDA is the only approved for evaluating treating myocardial ischemia damage over nearly 20 years
Test.
The formation of IMA is relevant with histanoxia.Human serum albumin (human serum albumin,
HSA) its amino terminal N-Asp-Ala-HIS-LYS is peculiar by the mankind, just
It is often cobalt (Co under physiological conditions++), copper (Cu++), nickel (Ni++) etc. transition metal ions
Principal binding sites, but easily by biochemical factors, be degraded such as the impact of pH etc., make HAS lose
Go and Co++Isoionic binding ability, forms IMA.
By research, Bar-Or etc. find that IMA raises rapidly after several minutes after tissue ischemia, it becomes
Change the most notable increasing in 6-24 hour before other index of cardiac muscle (CK-MB, Mb, cTnI) etc. increases
High.As the biochemical indicator of the most clinical myocardial ischemia uniquely specified, IMA has:
(1) early stage sensitive reflecting myocardium ischemic conditions.IMA is several minutes after myocardial ischemia occurs
I.e. raise, hence it is evident that being better than other biochemical indicator (can after there is myocardial necrosis after general 4-6 hour
Raise)
(2) significant negative prediction effect, contributes to clinical workers and Emergency Patients is carried out danger
Layering, getting rid of low danger patient ACS may.
(3) IMA concentration can be as the order of severity of myocardial ischemia and the judge index of prognosis.
Therefore, IMA is for the early diagnosis of acute myocardial ischemia, and ACS gets rid of diagnosis and ACS danger
Dangerous layered queuing networks, is respectively provided with significance.In clinical practice, fast for doubtful ACS patient
Speed carries out IMA detection, and clinical medical personnel can be helped to make patient in time, examine accurately
Break and implement active and effective therapeutic scheme.
It has been reported that the method for detection IMA has colorimetry, a liquid chromatography, mass spectroscopy and
Magnetic resonance detection method etc..In addition to colorimetry, remaining is all not suitable for routine clinical analysis.At present,
Colorimetric determination IMA test is included in routine clinical detection project by U.S. FDA, and it is commonly used
Technological means is:
1) instrument detection, the most large-scale biochemical analyzer, such as the Hitachi of Roche Holding Ag, Kang Basi
Detect on the multiple automatic analyzers such as the Cobas MIRA PLUS of company；
2) colorimetry kit of parts is analyzed by spectrophotometer or microplate reader detection.
The most traditional colorimetry or currently advanced automatic analytical instrument detect or test kit
Detection, all needs to carry out sample separation/Instrumental Analysis detection at laboratory, all can not meet clinic
The demand that myocardial ischemia is promptly and accurately judged by emergency treatment or different medical unit.
Summary of the invention
The technical problem to be solved is for above-mentioned the deficiencies in the prior art, it is provided that a kind of
The quickly chip of Ischemia modified albumin IMA in detection blood.This chip is based on Cobalt binding test
Principle, uses micro-total analysis system technology, is separated by sample and process conformity to is analyzed in detection
In device so that IMA detection is more convenient；This chip detecting method is simple, result is prone to judge,
It is suitable in the medical institutions such as basic hospital, emergency treatment, or the inpatient head of a bed uses, and makes medical personnel
Patient risk's layering can be judged rapidly, save medical resources and health care cost.
For solving above-mentioned technical problem, the technical solution used in the present invention is: one quickly detects blood
The chip of middle Ischemia modified albumin IMA, it is characterised in that include the base plate not absorbed water and the lid not absorbed water
Plate, described base plate is disposed with sample cell, mixing pit and detection cell, described sample cell with mix
It is provided with microchannel between pond and between mixing pit and detection cell, in described sample cell, is filled with sample
Product pad, described sample pad is coated with cobalt standard substance, is filled with detecting pad in described detection cell, described
Being coated with cobalt indicator on detecting pad, described cover plate is fixedly installed on above base plate, on described cover plate and
It is positioned at the opened above of sample cell and has well.
The chip of Ischemia modified albumin IMA in above-mentioned a kind of quick detection blood, it is characterised in that institute
State and on cover plate, offer steam vent.
The chip of Ischemia modified albumin IMA in above-mentioned a kind of quick detection blood, it is characterised in that institute
The quantity stating steam vent is multiple, and multiple described steam vents are respectively arranged at above sample cell, mixing pit
Above top and detection cell.
The chip of Ischemia modified albumin IMA in above-mentioned a kind of quick detection blood, it is characterised in that institute
State and on base plate, be provided with the sample cell air discharge duct being connected with sample cell, the mixing being connected with mixing pit
Pond air discharge duct, and the detection cell air discharge duct being connected with detection cell.
The chip of Ischemia modified albumin IMA in above-mentioned a kind of quick detection blood, it is characterised in that institute
The quantity stating steam vent is multiple, multiple described steam vents are respectively arranged at above sample cell air discharge duct,
Above mixing pit air discharge duct and above detection cell air discharge duct.
The chip of Ischemia modified albumin IMA in above-mentioned a kind of quick detection blood, it is characterised in that institute
State sample cell air discharge duct and be positioned on sample cell the one end near mixing pit.
The chip of Ischemia modified albumin IMA in above-mentioned a kind of quick detection blood, it is characterised in that institute
Stating cobalt standard substance is cobaltous chloride or cobaltous sulfate, and described cobalt indicator is 1-nitric acid Asia naphthoic acid, two sulfur threoses
Alcohol or 1,4-Dithioerythritol.
The chip of Ischemia modified albumin IMA in above-mentioned a kind of quick detection blood, it is characterised in that institute
The package amount stating cobalt standard substance is 1 μ L～100 μ L, and being coated concentration is 0.1mmol/L～15mmol/L.
The chip of Ischemia modified albumin IMA in above-mentioned a kind of quick detection blood, it is characterised in that institute
The package amount stating cobalt indicator is 1 μ L～100 μ L, and being coated concentration is 0.1mmol/L～15mmol/L.
The chip of Ischemia modified albumin IMA in above-mentioned a kind of quick detection blood, it is characterised in that institute
The material stating sample pad and detecting pad is fiber.
The present invention compared with prior art has the advantage that
1, the chip of the present invention is based on Cobalt binding test principle, uses micro-total analysis system
Technology, separates sample and detection is analyzed in process conformity to device.
2, the present invention is according to the principle of Cobalt binding test, by arrange sample cell, mixing pit,
Detection cell and microchannel system so that in Cobalt binding test, each step reaction can depend in order
Secondary generation.
3, the mixing pit of chip of the present invention is between sample cell and detection cell, gives detection sample and cobalt
Ions binding provides more buffering mixing space so that flow into the reason of sample in detection cell by microchannel
Change character more homogeneous, reduce technical error.
4, the present invention the most on the cover board arranges steam vent, it is possible to make detected sample and detectable
Flowing smoothly, and regulates each step reaction time.
5, the chip detecting method of the present invention is simple, result is prone to judge, be suitable for basic hospital,
The medical institutions such as emergency treatment, or the use of the inpatient head of a bed, make medical personnel can divide patient risk
Layer judges rapidly, save medical resources and health care cost.
6, the chip of the present invention is easy to use, with low cost, easily spreads to different medical unit and makes
With, meet the market demand, it is possible to fill up domestic and international market blank.
Below in conjunction with the accompanying drawings and embodiment, technical scheme is described in further detail.
Accompanying drawing explanation
Fig. 1 is the structural representation of the base plate of the embodiment of the present invention 1.
Fig. 2 is that the base plate of the embodiment of the present invention 1 removes sample pad and the structural representation of detecting pad.
Fig. 3 is the structural representation of the cover plate of the embodiment of the present invention 1.
Fig. 4 is the structural representation of the base plate of the embodiment of the present invention 2.
Fig. 5 is that the base plate of the embodiment of the present invention 2 removes sample pad and the structural representation of detecting pad.
Fig. 6 is the structural representation of the cover plate of the embodiment of the present invention 2.
Description of reference numerals:
1 base plate；2 cover plates；3 sample cells；
4 mixing pits；5 detection cells；6 sample pad；
7 detecting pads；8 wells；9 microchannels；
10 steam vents；11 sample cell air discharge ducts；12 mixing pit air discharge ducts；
13 detection cell air discharge ducts.
Detailed description of the invention
Embodiment 1
As shown in Figure 1, Figure 2 and Figure 3, deficiency decorated white egg in the quickly detection blood of the present embodiment
White chip, including the base plate 1 not absorbed water and the cover plate 2 not absorbed water, described base plate 1 sets successively
Be equipped with sample cell 3, mixing pit 4 and detection cell 5, between described sample cell 3 and mixing pit 4 and
It is provided with microchannel 9 between mixing pit 4 and detection cell 5, in described sample cell 3, is filled with sample
Pad 6, described sample pad 6 is coated with cobalt standard substance, is filled with detecting pad 7 in described detection cell 5,
Being coated with cobalt indicator on described detecting pad 7, described cover plate 2 is fixedly installed on above base plate 1, institute
State on cover plate 2 and be positioned at the opened above of sample cell 3 and have well 8.Described base plate and cover plate can be adopted
With material, egative film and the cover plates such as transparent glass, quartz, plastics or silica gel can by heat pressure adhesive or
The modes such as chemolysis are fixed together.
In the present embodiment, described cover plate 2 offers steam vent 10.
In the present embodiment, described base plate 1 is provided with the sample cell air discharge duct being connected with sample cell 3
11, the mixing pit air discharge duct 12 being connected with mixing pit 4, and the detection cell being connected with detection cell 5
Air discharge duct 13.
In the present embodiment, the quantity of described steam vent 10 is multiple (preferably 3～7), Duo Gesuo
State steam vent 10 be respectively arranged at above sample cell air discharge duct 11, above mixing pit air discharge duct 12 and
Above detection cell air discharge duct 13.
In the present embodiment, described sample cell air discharge duct 11 is positioned on sample cell 3 near mixing pit 4
One end.
In the present embodiment, described cobalt standard substance are cobaltous chloride or cobaltous sulfate, and described cobalt indicator is 1-
Nitric acid Asia naphthoic acid, dithiothreitol, DTT or 1,4-Dithioerythritol.
In the present embodiment, the package amount of described cobalt standard substance is 1 μ L～100 μ L, is coated concentration and is
0.1mmol/L～15mmol/L.
In the present embodiment, the package amount of described cobalt indicator is 1 μ L～100 μ L, is coated concentration and is
0.1mmol/L～15mmol/L.
In the present embodiment, the material of described sample pad 6 and detecting pad 7 is fiber, can use natural
Fiber or synthetic fibers.
In the present embodiment, the preferred package amount of cobalt standard substance and cobalt indicator and be coated concentration and all see below
Table.
The cobalt standard substance of table 1 embodiment 1 and the preferred package amount of cobalt indicator and be coated concentration
Embodiment 2
As shown in Figure 4, Figure 5 and Figure 6, deficiency decorated white egg in the quickly detection blood of the present embodiment
White chip, including the base plate 1 not absorbed water and the cover plate 2 not absorbed water, described base plate 1 sets successively
Be equipped with sample cell 3, mixing pit 4 and detection cell 5, between described sample cell 3 and mixing pit 4 and
It is provided with microchannel 9 between mixing pit 4 and detection cell 5, in described sample cell 3, is filled with sample
Pad 6, described sample pad 6 is coated with cobalt standard substance, is filled with detecting pad 7 in described detection cell 5,
Being coated with cobalt indicator on described detecting pad 7, described cover plate 2 is fixedly installed on above base plate 1, institute
State on cover plate 2 and be positioned at the opened above of sample cell 3 and have well 8.Described base plate and cover plate can be adopted
With material, egative film and the cover plates such as transparent glass, quartz, plastics or silica gel can by heat pressure adhesive or
The modes such as chemolysis are fixed together.
In the present embodiment, described cover plate 2 offers steam vent 10.
In the present embodiment, the quantity of described steam vent 10 is multiple (preferably 3～7), Duo Gesuo
State steam vent 10 to be respectively arranged at above sample cell 3, above mixing pit 4 and above detection cell 5.
In the present embodiment, described cobalt standard substance are cobaltous chloride or cobaltous sulfate, and described cobalt indicator is 1-
Nitric acid Asia naphthoic acid, dithiothreitol, DTT or 1,4-Dithioerythritol.
In the present embodiment, the package amount of described cobalt standard substance is 1 μ L～100 μ L, is coated concentration and is
0.1mmol/L～15mmol/L.
In the present embodiment, the package amount of described cobalt indicator is 1 μ L～100 μ L, is coated concentration and is
0.1mmol/L～15mmol/L.
In the present embodiment, the material of described sample pad 6 and detecting pad 7 is fiber, can use natural
Fiber or synthetic fibers.
In the present embodiment, the preferred package amount of cobalt standard substance and cobalt indicator and be coated concentration and all see below
Table.
The cobalt standard substance of table 2 embodiment 2 and the preferred package amount of cobalt indicator and be coated concentration
The mentality of designing of the chip of the present invention is not help complete independently IMA by any instrument
Detection, its principle be by the change of cobalt ion binding ability in assessment detection sample and cobalt standard substance from
And judge to detect whether IMA in sample raises.
The using method of chip of the present invention is as follows: add 25 μ L in sample pad 6 by sample well 8
Blood serum sample or 40 μ L without anticoagulant, without the whole blood sample of anticoagulant, then add in sample pad 6
Enter 30 μ L detectable, stand 5 minutes；50 μ L detectable it are added dropwise over again in sample pad 6,
Stand 5 minutes；Finally in 1 minute, in sample pad 6, it is added dropwise over 100 μ L detectable, quiet
Observe the color change of detecting pad 7 after putting 10 minutes, and compare with standard colorimetric plate, it is judged that test
IMA concentration in sample.Described detectable is the Tris-HCl of pH value 7.8.
Standard colorimetric plate is prepared by following methods:
As a example by the chip of the embodiment of the present invention 1, prepare respectively containing different cobalt standard substance and cobalt instruction
The chip of agent, detects the relation between quality and the volume of detected sample of cobalt in cobalt standard substance,
Go out volume (unit is μ L) and the quality (unit is ng) of cobalt in cobalt standard substance of detected sample
Preferred proportion be 1:(1～5)；40 μ L whole bloods or 25 μ L blood serum samples are applied to test, when
When the concentration of cobalt indicator is 1 μM～2000 μMs, in cobalt standard substance concentration of cobalt ions be 0～
When 2000 μMs, the intensity of cobalt indicator color change is directly proportional to the concentration of cobalt ion and easily distinguishes
Know；Preparation concentration of cobalt ions is the cobalt standard substance of five Concentraton gradient of 0～1000 μM, selection etc.
The sample pad of the cobalt standard substance envelope chip of amount variable concentrations, depends on according to cobalt standard concentration is ascending
Secondary it is labeled as SD1, SD2, SD3, SD4 and SD5, carries out for detected sample with 25 μ L serum
Detection, the result of gained is recorded as successively ,+, ++, +++ and ++++, show according to detecting pad
Color prepare standard colorimetric plate.
Result verification:
1, the IMA standard substance using 180U/mL are raw material, and normal saline is diluent, prepares dense
Degree be respectively 180U/mL, 145U/mL, 120U/mL, 90U/mL, 60U/mL, 45U/mL and
The IMA sample of 0U/mL.
According to the using method of the invention described above chip, the IMA sample of the variable concentrations of preparation is carried out
Detection, standard colorimetric plate prepared by result and said method contrasts, result such as following table.
The testing result of table 3 variable concentrations IMA sample
2, the IMA standard substance (HSD) using 180U/mL are raw material, use healthy pooled serum
(HS) it is that diluent carries out serial dilution to IMA standard substance, according to making of the invention described above chip
Detecting the IMA sample of healthy pooled serum dilution by method, result is prepared with said method
Standard colorimetric plate contrast, result such as following table.
The testing result of the IMA sample of the different dilution ratio of table 4
Tested by two above it can be seen that the present invention chip detection result and detected sample in
IMA concentration relevant.
Comparison and detection:
Choose commercially available IMA detection kit (Shanghai Wei Yin Bioisystech Co., Ltd), according to saying
The IMA level of bright book random detection 20 example suspected ACS patient, take simultaneously peripheral blood by
Chip detection patient's IMA level according to the using method present invention of the invention described above chip.Result
As follows:
Table 5 commercially available IMA detection kit and the testing result of chip of the present invention
(the cut-off value of commercially available IMA detection kit manufacturer suggestion is 67U/mL)
By above contrast test it can be seen that chip of the present invention can detect IMA in different sample
Concentration change, testing result product existing with market result is consistent.
Influence factor:
The impact of chip detection result of the present invention is analyzed by different affecting factors.
1, seralbumin concentration
The testing result of Ischemia modified albumin IMA is directly affected by seralbumin concentration.According to existing
Report, during the detection of Ischemia modified albumin IMA, when seralbumin concentration at 20g/L～
During 55g/L, the impact of IMA testing result is negligible by seralbumin concentration.The present invention
By serial dilution human serum albumin solution, obtaining concentration is 20g/L, 30g/L, 40g/L and 50g/L
Albumin solution, use the invention described above chip using method detect, result shows white egg
White diluted sample testing result indifference.
2, hemoglobin/haemolysis sample
IMA high level sample (180U/mL) is become 120U/mL, 60U/mL with normal saline dilution
Sample, and it is separately added into the hemoglobin of serial dilution, making hemoglobin concentration is 0～160g/L,
Apply chip detection of the present invention and observe testing result.Hemoglobin concentration is at 0～160g/L concentration model
Enclose interior on chip I MA testing result of the present invention without impact.
3, triglyceride (TG)
It is separately added into not in IMA high level sample (120U/mL) with low value sample (60U/mL)
Commensurability triglyceride, makes triglyceride concentration between 0～800mmol/L.Apply core of the present invention
Sheet detects and observes testing result change.Triglyceride concentration is when≤800mmol/L, to the present invention
Chip detection result is without impact.
Stability experiment:
After chip of the present invention is dried sealing, it is placed in 37 DEG C of calorstats 14 days, then takes out, balance
To room temperature；After the chip of same product batch number is dried sealing, 2 DEG C～8 DEG C preservations, take out before test,
Balance is to room temperature；Then the testing result of the chip of different store methods is compared.Result shows 37 DEG C of storages
Deposit 14 days to chip detection result of the present invention without impact.
The chip of the present invention not only can use range estimation to compare the height of IMA concentration in detection sample, also
Mobile phone photograph can be used to transfer data to data center carry out interpretation of result and compare.It addition, also can design
Chip reading machine carries out data analysis and converses IMA numerical value according to standard curve so that the present invention
The concrete digitized of testing result of chip.
The above, be only presently preferred embodiments of the present invention, and the present invention not does any restriction, all
It is any simple modification, change and equivalence knot above example made according to inventive technique essence
Structure changes, and all still falls within the protection domain of technical solution of the present invention.
Claims (10)
1. the chip of Ischemia modified albumin IMA in a quick detection blood, it is characterised in that include
The base plate (1) not absorbed water and the cover plate (2) not absorbed water, described base plate is disposed with on (1)
Sample cell (3), mixing pit (4) and detection cell (5), described sample cell (3) and mixing pit (4)
Between and mixing pit (4) and detection cell (5) between be provided with microchannel (9), described sample
Being filled with sample pad (6) in product pond (3), described sample pad is coated with cobalt standard substance on (6),
It is filled with detecting pad (7) in described detection cell (5), described detecting pad (7) is coated with cobalt and refers to
Show that agent, described cover plate (2) are fixedly installed on base plate (1) top, on described cover plate (2) and position
Opened above in sample cell (3) has well (8).
The core of Ischemia modified albumin IMA in a kind of quick detection blood the most according to claim 1
Sheet, it is characterised in that offer steam vent (10) on described cover plate (2).
The core of Ischemia modified albumin IMA in a kind of quick detection blood the most according to claim 2
Sheet, it is characterised in that the quantity of described steam vent (10) is multiple, multiple described steam vents (10)
It is respectively arranged at sample cell (3) top, mixing pit (4) top and detection cell (5) top.
The core of Ischemia modified albumin IMA in a kind of quick detection blood the most according to claim 2
Sheet, it is characterised in that be provided with the sample cell being connected with sample cell (3) on described base plate (1)
Air discharge duct (11), the mixing pit air discharge duct (12) being connected with mixing pit (4), and with detection
The detection cell air discharge duct (13) that pond (5) is connected.
The core of Ischemia modified albumin IMA in a kind of quick detection blood the most according to claim 4
Sheet, it is characterised in that the quantity of described steam vent (10) is multiple, multiple described steam vents (10)
It is respectively arranged at sample cell air discharge duct (11) top, mixing pit air discharge duct (12) top and detection cell
Air discharge duct (13) top.
The core of Ischemia modified albumin IMA in a kind of quick detection blood the most according to claim 5
Sheet, it is characterised in that it is upper near mixing that described sample cell air discharge duct (11) is positioned at sample cell (3)
The one end in pond (4).
The core of Ischemia modified albumin IMA in a kind of quick detection blood the most according to claim 1
Sheet, it is characterised in that described cobalt standard substance are cobaltous chloride or cobaltous sulfate, described cobalt indicator is 1-
Nitric acid Asia naphthoic acid, dithiothreitol, DTT or 1,4-Dithioerythritol.
The core of Ischemia modified albumin IMA in a kind of quick detection blood the most according to claim 7
Sheet, it is characterised in that the package amount of described cobalt standard substance is 1 μ L～100 μ L, is coated concentration and is
0.1mmol/L～15mmol/L.
The core of Ischemia modified albumin IMA in a kind of quick detection blood the most according to claim 7
Sheet, it is characterised in that the package amount of described cobalt indicator is 1 μ L～100 μ L, is coated concentration and is
0.1mmol/L～15mmol/L.
The core of Ischemia modified albumin IMA in a kind of quick detection blood the most according to claim 1
Sheet, it is characterised in that the material of described sample pad (6) and detecting pad (7) is fiber.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070053796A1 (en) * | 2005-09-02 | 2007-03-08 | Jen-Jr Gau | Cartridge having variable volume reservoirs |
| CN101013137A (en) * | 2007-02-06 | 2007-08-08 | 贺坚慧 | Reagent casing for detecting blood-lacking modification albumin and method thereof |
| CN103760357A (en) * | 2013-11-04 | 2014-04-30 | 山东博科生物产业有限公司 | Detection kit for ischemia modified albumin |
| CN204594998U (en) * | 2015-04-29 | 2015-08-26 | 中国检验检疫科学研究院 | A kind of Coxiella burnetii antibody assay kit |
| CN105597846A (en) * | 2015-10-26 | 2016-05-25 | 深圳华迈兴微医疗科技有限公司 | Magnetic particle chemiluminescence microfluidic chip for quantitative detection of D-dimer |
| CN205982097U (en) * | 2016-07-21 | 2017-02-22 | 韩雅君 | Lack chip that albumin was decoratied to blood among quick detect blood |
-
2016
- 2016-07-21 CN CN201610579482.XA patent/CN105973887B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070053796A1 (en) * | 2005-09-02 | 2007-03-08 | Jen-Jr Gau | Cartridge having variable volume reservoirs |
| CN101013137A (en) * | 2007-02-06 | 2007-08-08 | 贺坚慧 | Reagent casing for detecting blood-lacking modification albumin and method thereof |
| CN103760357A (en) * | 2013-11-04 | 2014-04-30 | 山东博科生物产业有限公司 | Detection kit for ischemia modified albumin |
| CN204594998U (en) * | 2015-04-29 | 2015-08-26 | 中国检验检疫科学研究院 | A kind of Coxiella burnetii antibody assay kit |
| CN105597846A (en) * | 2015-10-26 | 2016-05-25 | 深圳华迈兴微医疗科技有限公司 | Magnetic particle chemiluminescence microfluidic chip for quantitative detection of D-dimer |
| CN205982097U (en) * | 2016-07-21 | 2017-02-22 | 韩雅君 | Lack chip that albumin was decoratied to blood among quick detect blood |
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| CN105973887B (en) | 2018-09-18 |
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